Restriction map for
pF1KA1181
(Circular) (Six-base) MAPSORT of: pF1KA1181.seq Check: 9767 from: 1 to: 3983
pF1KA1181 3983 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AatII G_ACGT'C
Cuts at: 320 320
Size: 3983
AccI GT'mk_AC
Cuts at: 1021 1021
Size: 3983
Acc65I G'GTAC_C
Cuts at: 999 999
Size: 3983
AclI AA'CG_TT
Cuts at: 3823 3823
Size: 3983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 694 1808 2240 2987 3255 105
Size: 589 1114 432 747 268 833
Fragments arranged by size:
1114 833 747 589 432 268
AflIII A'CryG_T
Cuts at: 500 1335 2439 3416 500
Size: 835 1104 977 1067
Fragments arranged by size:
1104 1067 977 835
AgeI A'CCGG_T
Cuts at: 2371 2371
Size: 3983
AlwNI CAG_nnn'CTG
Cuts at: 852 940 1288 2855 852
Size: 88 348 1567 1980
Fragments arranged by size:
1980 1567 348 88
ApaLI G'TGCA_C
Cuts at: 2753 2753
Size: 3983
ApoI r'AATT_y
Cuts at: 987 3336 3592 987
Size: 2349 256 1378
Fragments arranged by size:
2349 1378 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3983
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
AvaI C'yCGr_G
Cuts at: 619 1003 619
Size: 384 3599
BamHI G'GATC_C
Cuts at: 1008 1008
Size: 3983
BanI G'GyrC_C
Cuts at: 147 999 1413 1661 1696 147
Size: 852 414 248 35 2434
Fragments arranged by size:
2434 852 414 248 35
BanII G_rGCy'C
Cuts at: 239 275 997 2027 239
Size: 36 722 1030 2195
Fragments arranged by size:
2195 1030 722 36
BbeI G_GCGC'C
Cuts at: 1417 1665 1417
Size: 248 3735
BbvCI CC'TCA_GC
Cuts at: 811 811
Size: 3983
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3949
BciVI GTATCCnnnnn_n'
Cuts at: 1881 2642 3983 1881
Size: 761 1341 1881
Fragments arranged by size:
1881 1341 761
BclI T'GATC_A
Cuts at: 1503 1503
Size: 3983
BfrBI ATG'CAT
Cuts at: 926 975 926
Size: 49 3934
BglI GCCn_nnn'nGGC
Cuts at: 773 943 773
Size: 170 3813
BglII A'GATC_T
Cuts at: 1498 3535 1498
Size: 2037 1946
BlpI GC'TnA_GC
Cuts at: 1095 1095
Size: 3983
Bme1580I G_kGCm'C
Cuts at: 152 686 1608 1701 2757 152
Size: 534 922 93 1056 1378
Fragments arranged by size:
1378 1056 922 534 93
BmrI ACTGGGnnnn_n'
Cuts at: 1195 1276 1611 3755 1195
Size: 81 335 2144 1423
Fragments arranged by size:
2144 1423 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 3983
Bpu10I CC'TnA_GC
Cuts at: 811 811
Size: 3983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 2038 2530 2828 1159
Size: 879 492 298 2314
Fragments arranged by size:
2314 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3687 3687
Size: 3983
BsaAI yAC'GTr
Cuts at: 501 1336 501
Size: 835 3148
BsaBI GATnn'nnATC
Cuts at: 6 1497 3534 6
Size: 1491 2037 455
Fragments arranged by size:
2037 1491 455
BsaHI Gr'CG_yC
Cuts at: 317 1414 1662 2364 3860 317
Size: 1097 248 702 1496 440
Fragments arranged by size:
1496 1097 702 440 248
BsaWI w'CCGG_w
Cuts at: 241 1172 1693 2371 2645 2792 3203 241
Size: 931 521 678 274 147 411 1021
Fragments arranged by size:
1021 931 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 550 580 550
Size: 30 3953
BseYI C'CCAG_C
Cuts at: 524 963 1408 2166 2743 3239 524
Size: 439 445 758 577 496 1268
Fragments arranged by size:
1268 758 577 496 445 439
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 331
Size: 3983
BsiEI CG_ry'CG
Cuts at: 104 1525 1571 2779 104
Size: 1421 46 1208 1308
Fragments arranged by size:
1421 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 239 275 997 1775 1965 2757 239
Size: 36 722 778 190 792 1465
Fragments arranged by size:
1465 792 778 722 190 36
BsmBI CGTCTCn'nnnn_
Cuts at: 834 834
Size: 3983
Bsp1286I G_dGCh'C
Cuts at: 152 239 275 686 997 1608 1701 1775 1965
Size: 87 36 411 311 611 93 74 190
Cuts at: 1965 2027 2757 152
Size: 62 730 1378
Fragments arranged by size:
1378 730 611 411 311 190 93 87
74 62 36
BspEI T'CCGG_A
Cuts at: 3203 3203
Size: 3983
BspHI T'CATG_A
Cuts at: 3159 3159
Size: 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1033 1247 1549 1930 1033
Size: 214 302 381 3086
Fragments arranged by size:
3086 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2275 2329 3813 3978 50
Size: 2225 54 1484 165 55
Fragments arranged by size:
2225 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1895 1895
Size: 3983
BsrFI r'CCGG_y
Cuts at: 907 1981 2162 2371 907
Size: 1074 181 209 2519
Fragments arranged by size:
2519 1074 209 181
BsrGI T'GTAC_A
Cuts at: 854 854
Size: 3983
BssSI C'ACGA_G
Cuts at: 2254 2612 2254
Size: 358 3625
BstAPI GCAn_nnn'nTGC
Cuts at: 1360 1360
Size: 3983
BstBI TT'CG_AA
Cuts at: 2344 2344
Size: 3983
BstXI CCAn_nnnn'nTGG
Cuts at: 651 651
Size: 3983
BstYI r'GATC_y
Cuts at: 1 425 956 1008 1475 1498 1833 2079 3091
Size: 424 531 52 467 23 335 246 1012
Cuts at: 3091 3177 3189 3535 1
Size: 86 12 346 449
Fragments arranged by size:
1012 531 467 449 424 346 335 246
86 52 23 12
BtgI C'CryG_G
Cuts at: 600 686 600
Size: 86 3897
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1933 2114 2277 1933
Size: 181 163 3639
Fragments arranged by size:
3639 181 163
BtsI GCAGTG_nn'
Cuts at: 348 1226 348
Size: 878 3105
DraI TTT'AAA
Cuts at: 982 982
Size: 3983
DraIII CAC_nnn'GTG
Cuts at: 399 514 399
Size: 115 3868
DrdI GACnn_nn'nnGTC
Cuts at: 1689 2547 1689
Size: 858 3125
EaeI y'GGCC_r
Cuts at: 740 1568 1742 2133 2160 3505 740
Size: 828 174 391 27 1345 1218
Fragments arranged by size:
1345 1218 828 391 174 27
EagI C'GGCC_G
Cuts at: 1568 1568
Size: 3983
EarI CTCTTCn'nnn_
Cuts at: 214 223 229 2006 2216 214
Size: 9 6 1777 210 1981
Fragments arranged by size:
1981 1777 210 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2191 2501 2647 3793 2191
Size: 310 146 1146 2381
Fragments arranged by size:
2381 1146 310 146
EcoICRI GAG'CTC
Cuts at: 237 273 995 237
Size: 36 722 3225
Fragments arranged by size:
3225 722 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 694 1808 2240 2987 3255 105
Size: 589 1114 432 747 268 833
Fragments arranged by size:
1114 833 747 589 432 268
EcoO109I rG'GnC_Cy
Cuts at: 156 483 893 1122 156
Size: 327 410 229 3017
Fragments arranged by size:
3017 410 327 229
EcoRI G'AATT_C
Cuts at: 987 987
Size: 3983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 585 2002 585
Size: 1417 2566
FspI TGC'GCA
Cuts at: 1764 1764
Size: 3983
HaeII r_GCGC'y
Cuts at: 1417 1665 2687 3666 1417
Size: 248 1022 979 1734
Fragments arranged by size:
1734 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1825 1855 1857 1887 1825
Size: 30 2 30 3921
Fragments arranged by size:
3921 30 30 2
HincII GTy'rAC
Cuts at: 1022 1022
Size: 3983
KasI G'GCGC_C
Cuts at: 1413 1661 1413
Size: 248 3735
KpnI G_GTAC'C
Cuts at: 1003 1003
Size: 3983
MluI A'CGCG_T
Cuts at: 3416 3416
Size: 3983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 668 2654 2838 668
Size: 1986 184 1813
Fragments arranged by size:
1986 1813 184
MscI TGG'CCA
Cuts at: 742 1744 742
Size: 1002 2981
MslI CAynn'nnrTG
Cuts at: 1972 2099 1972
Size: 127 3856
MspA1I CmG'CkG
Cuts at: 302 432 717 852 963 1094 1408 1768 2781
Size: 130 285 135 111 131 314 360 1013
Cuts at: 2781 3026 302
Size: 245 1259
Fragments arranged by size:
1259 1013 360 314 285 245 135 131
130 111
NaeI GCC'GGC
Cuts at: 909 2164 909
Size: 1255 2728
NarI GG'CG_CC
Cuts at: 1414 1662 1414
Size: 248 3735
NgoMIV G'CCGG_C
Cuts at: 907 2162 907
Size: 1255 2728
NsiI A_TGCA'T
Cuts at: 928 977 928
Size: 49 3934
NspI r_CATG'y
Cuts at: 553 979 1036 1536 2443 553
Size: 426 57 500 907 2093
Fragments arranged by size:
2093 907 500 426 57
PciI A'CATG_T
Cuts at: 2439 2439
Size: 3983
PfoI T'CCnGG_A
Cuts at: 914 914
Size: 3983
PmeI GTTT'AAAC
Cuts at: 982 982
Size: 3983
PmlI CAC'GTG
Cuts at: 501 501
Size: 3983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 451 483 3122 3154 451
Size: 32 2639 32 1280
Fragments arranged by size:
2639 1280 32 32
PpuMI rG'GwC_Cy
Cuts at: 156 483 893 156
Size: 327 410 3246
Fragments arranged by size:
3246 410 327
PshAI GACnn'nnGTC
Cuts at: 320 550 320
Size: 230 3753
PsiI TTA'TAA
Cuts at: 250 250
Size: 3983
PstI C_TGCA'G
Cuts at: 1030 1715 1030
Size: 685 3298
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3983
PvuII CAG'CTG
Cuts at: 963 1408 1768 963
Size: 445 360 3178
Fragments arranged by size:
3178 445 360
RsrII CG'GwC_CG
Cuts at: 2178 2178
Size: 3983
SacI G_AGCT'C
Cuts at: 239 275 997 239
Size: 36 722 3225
Fragments arranged by size:
3225 722 36
SalI G'TCGA_C
Cuts at: 1020 1020
Size: 3983
SapI GCTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
SbfI CC_TGCA'GG
Cuts at: 1030 1030
Size: 3983
ScaI AGT'ACT
Cuts at: 3500 3500
Size: 3983
SfcI C'TryA_G
Cuts at: 33 138 583 762 1026 1711 2704 2895 33
Size: 105 445 179 264 685 993 191 1121
Fragments arranged by size:
1121 993 685 445 264 191 179 105
SfoI GGC'GCC
Cuts at: 1415 1663 1415
Size: 248 3735
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
SmaI CCC'GGG
Cuts at: 1005 1005
Size: 3983
SmlI C'TyrA_G
Cuts at: 1138 2053 2545 2807 1138
Size: 915 492 262 2314
Fragments arranged by size:
2314 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3983
SphI G_CATG'C
Cuts at: 1036 1536 1036
Size: 500 3483
StuI AGG'CCT
Cuts at: 946 946
Size: 3983
StyI C'CwwG_G
Cuts at: 152 1117 1471 152
Size: 965 354 2664
Fragments arranged by size:
2664 965 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1566 1699 2158 2367 1566
Size: 133 459 209 3182
Fragments arranged by size:
3182 459 209 133
TatI w'GTAC_w
Cuts at: 731 830 854 3498 731
Size: 99 24 2644 1216
Fragments arranged by size:
2644 1216 99 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 550 582 3500 3532 550
Size: 32 2918 32 1001
Fragments arranged by size:
2918 1001 32 32
Tth111I GACn'n_nGTC
Cuts at: 1780 1780
Size: 3983
XbaI T'CTAG_A
Cuts at: 1014 1014
Size: 3983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1478 1478
Size: 3983
XmaI C'CCGG_G
Cuts at: 1003 1003
Size: 3983
ZraI GAC'GTC
Cuts at: 318 318
Size: 3983
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BmgBI BmtI BpmI BseRI
BsiWI BsmI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI PacI PasI PflMI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI