Restriction map for
pF1KA1182
(Circular) (Six-base) MAPSORT of: pF1KA1182.seq Check: 562 from: 1 to: 4853
pF1KA1182 4853 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 473 984 1079 1505 1646 473
Size: 511 95 426 141 3680
Fragments arranged by size:
3680 511 426 141 95
AccI GT'mk_AC
Cuts at: 1891 1891
Size: 4853
Acc65I G'GTAC_C
Cuts at: 1869 1869
Size: 4853
AclI AA'CG_TT
Cuts at: 4693 4693
Size: 4853
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 223 511 621 622 2678 3110 3463 4125
Size: 48 288 110 1 2056 432 353 662
Cuts at: 4125 175
Size: 903
Fragments arranged by size:
2056 903 662 432 353 288 110 48
1
AfeI AGC'GCT
Cuts at: 630 630
Size: 4853
AflIII A'CryG_T
Cuts at: 2205 4005 4286 2205
Size: 1800 281 2772
Fragments arranged by size:
2772 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 129 717 771 999 1080 2158 3596 129
Size: 588 54 228 81 1078 1438 1386
Fragments arranged by size:
1438 1386 1078 588 228 81 54
ApaLI G'TGCA_C
Cuts at: 802 1606 3691 802
Size: 804 2085 1964
Fragments arranged by size:
2085 1964 804
ApoI r'AATT_y
Cuts at: 1531 1857 4206 4462 1531
Size: 326 2349 256 1922
Fragments arranged by size:
2349 1922 326 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4853
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4853
AvaI C'yCGr_G
Cuts at: 1444 1873 1444
Size: 429 4424
BamHI G'GATC_C
Cuts at: 1878 1878
Size: 4853
BanI G'GyrC_C
Cuts at: 692 899 1067 1869 2283 2531 2566 692
Size: 207 168 802 414 248 35 2979
Fragments arranged by size:
2979 802 414 248 207 168 35
BanII G_rGCy'C
Cuts at: 650 1196 1214 1867 2897 650
Size: 546 18 653 1030 2606
Fragments arranged by size:
2606 1030 653 546 18
BbeI G_GCGC'C
Cuts at: 696 2287 2535 696
Size: 1591 248 3014
Fragments arranged by size:
3014 1591 248
BbsI GAAGACnn'nnnn_
Cuts at: 395 1176 395
Size: 781 4072
BbvCI CC'TCA_GC
Cuts at: 365 1292 365
Size: 927 3926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1516 1545 1550 1579 1516
Size: 29 5 29 4790
Fragments arranged by size:
4790 29 29 5
BciVI GTATCCnnnnn_n'
Cuts at: 2751 3807 4853 2751
Size: 1056 1046 2751
Fragments arranged by size:
2751 1056 1046
BclI T'GATC_A
Cuts at: 2373 2373
Size: 4853
BglII A'GATC_T
Cuts at: 551 2368 4405 551
Size: 1817 2037 999
Fragments arranged by size:
2037 1817 999
BlpI GC'TnA_GC
Cuts at: 824 1965 824
Size: 1141 3712
Bme1580I G_kGCm'C
Cuts at: 806 1610 1832 2478 2571 3695 806
Size: 804 222 646 93 1124 1964
Fragments arranged by size:
1964 1124 804 646 222 93
BmgBI CAC'GTC
Cuts at: 855 855
Size: 4853
BmrI ACTGGGnnnn_n'
Cuts at: 1300 2065 2146 2481 4625 1300
Size: 765 81 335 2144 1528
Fragments arranged by size:
2144 1528 765 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 428 1746 3220 428
Size: 1318 1474 2061
Fragments arranged by size:
2061 1474 1318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 349 418 637 1687 1708 1828 304
Size: 45 69 219 1050 21 120 3329
Fragments arranged by size:
3329 1050 219 120 69 45 21
Bpu10I CC'TnA_GC
Cuts at: 365 1292 365
Size: 927 3926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 1561 2029 2908 3622 3920 172
Size: 1389 468 879 714 298 1105
Fragments arranged by size:
1389 1105 879 714 468 298
BsaI GGTCTCn'nnnn_
Cuts at: 154 1450 4557 154
Size: 1296 3107 450
Fragments arranged by size:
3107 1296 450
BsaAI yAC'GTr
Cuts at: 2206 2206
Size: 4853
BsaBI GATnn'nnATC
Cuts at: 6 2367 4404 6
Size: 2361 2037 455
Fragments arranged by size:
2361 2037 455
BsaHI Gr'CG_yC
Cuts at: 274 693 908 974 1798 2284 2532 3234 4730
Size: 419 215 66 824 486 248 702 1496
Cuts at: 4730 274
Size: 397
Fragments arranged by size:
1496 824 702 486 419 397 248 215
66
BsaWI w'CCGG_w
Cuts at: 477 1766 2042 2563 3241 3652 3799 4073 477
Size: 1289 276 521 678 411 147 274 1257
Fragments arranged by size:
1289 1257 678 521 411 276 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 322 352 1572 1602 1700 1730 322
Size: 30 1220 30 98 30 3445
Fragments arranged by size:
3445 1220 98 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1309 1420 1618 1309
Size: 111 198 4544
Fragments arranged by size:
4544 198 111
BseYI C'CCAG_C
Cuts at: 999 1146 1733 1774 1822 2278 3036 3701 4109
Size: 147 587 41 48 456 758 665 408
Cuts at: 4109 999
Size: 1743
Fragments arranged by size:
1743 758 665 587 456 408 147 48
41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 250 643 730 795 826 852 1381 1624
Size: 91 393 87 65 31 26 529 243
Cuts at: 1624 159
Size: 3388
Fragments arranged by size:
3388 529 393 243 91 87 65 31
26
BsiEI CG_ry'CG
Cuts at: 104 2395 2441 3671 104
Size: 2291 46 1230 1286
Fragments arranged by size:
2291 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 650 806 1060 1214 1610 1867 2645 2835 3695
Size: 156 254 154 396 257 778 190 860
Cuts at: 3695 650
Size: 1808
Fragments arranged by size:
1808 860 778 396 257 254 190 156
154
BsmBI CGTCTCn'nnnn_
Cuts at: 1789 1789
Size: 4853
Bsp1286I G_dGCh'C
Cuts at: 650 806 1060 1196 1214 1610 1832 1867 2478
Size: 156 254 136 18 396 222 35 611
Cuts at: 2478 2571 2645 2835 2897 3695 650
Size: 93 74 190 62 798 1808
Fragments arranged by size:
1808 798 611 396 254 222 190 156
136 93 74 62 35 18
BspEI T'CCGG_A
Cuts at: 477 477
Size: 4853
BspHI T'CATG_A
Cuts at: 455 3285 455
Size: 2830 2023
BspMI ACCTGCnnnn'nnnn_
Cuts at: 272 473 984 1079 1505 1646 1903 2117 2419
Size: 201 511 95 426 141 257 214 302
Cuts at: 2419 2800 272
Size: 381 2325
Fragments arranged by size:
2325 511 426 381 302 257 214 201
141 95
BsrBI CCG'CTC
Cuts at: 50 186 924 1706 1845 3145 3199 4683 4848
Size: 136 738 782 139 1300 54 1484 165
Cuts at: 4848 50
Size: 55
Fragments arranged by size:
1484 1300 782 738 165 139 136 55
54
BsrDI GCAATG_nn'
Cuts at: 2765 2765
Size: 4853
BsrFI r'CCGG_y
Cuts at: 1610 1800 2851 3032 1610
Size: 190 1051 181 3431
Fragments arranged by size:
3431 1051 190 181
BssSI C'ACGA_G
Cuts at: 3124 3832 3124
Size: 708 4145
BstAPI GCAn_nnn'nTGC
Cuts at: 771 1370 1620 1789 2230 771
Size: 599 250 169 441 3394
Fragments arranged by size:
3394 599 441 250 169
BstBI TT'CG_AA
Cuts at: 3214 3214
Size: 4853
BstYI r'GATC_y
Cuts at: 1 551 1878 2345 2368 2703 2949 3255 3267
Size: 550 1327 467 23 335 246 306 12
Cuts at: 3267 3353 4405 1
Size: 86 1052 449
Fragments arranged by size:
1327 1052 550 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 277 440 533 872 277
Size: 163 93 339 4258
Fragments arranged by size:
4258 339 163 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 330 2803 2984 3147 330
Size: 2473 181 163 2036
Fragments arranged by size:
2473 2036 181 163
BtsI GCAGTG_nn'
Cuts at: 1096 1721 2096 1096
Size: 625 375 3853
Fragments arranged by size:
3853 625 375
DraI TTT'AAA
Cuts at: 1852 1852
Size: 4853
DraIII CAC_nnn'GTG
Cuts at: 899 899
Size: 4853
DrdI GACnn_nn'nnGTC
Cuts at: 1180 2559 3903 1180
Size: 1379 1344 2130
Fragments arranged by size:
2130 1379 1344
EaeI y'GGCC_r
Cuts at: 137 1086 1523 2438 2612 3003 3030 4375 137
Size: 949 437 915 174 391 27 1345 615
Fragments arranged by size:
1345 949 915 615 437 391 174 27
EagI C'GGCC_G
Cuts at: 2438 2438
Size: 4853
EarI CTCTTCn'nnn_
Cuts at: 242 397 580 640 787 1567 2876 3086 242
Size: 155 183 60 147 780 1309 210 2009
Fragments arranged by size:
2009 1309 780 210 183 155 147 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1423 3061 3803 3949 4663 1423
Size: 1638 742 146 714 1613
Fragments arranged by size:
1638 1613 742 714 146
EcoICRI GAG'CTC
Cuts at: 648 1212 1865 648
Size: 564 653 3636
Fragments arranged by size:
3636 653 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 223 304 349 418 511 621 622 637
Size: 48 81 45 69 93 110 1 15
Cuts at: 637 1687 1708 1828 2678 3110 3463 4125 175
Size: 1050 21 120 850 432 353 662 903
Fragments arranged by size:
1050 903 850 662 432 353 120 110
93 81 69 48 45 21 15 1
EcoNI CCTnn'n_nnAGG
Cuts at: 469 619 1590 469
Size: 150 971 3732
Fragments arranged by size:
3732 971 150
EcoO109I rG'GnC_Cy
Cuts at: 126 150 570 1005 1683 1992 126
Size: 24 420 435 678 309 2987
Fragments arranged by size:
2987 678 435 420 309 24
EcoRI G'AATT_C
Cuts at: 1857 1857
Size: 4853
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 4853
FspI TGC'GCA
Cuts at: 775 790 2634 775
Size: 15 1844 2994
Fragments arranged by size:
2994 1844 15
HaeII r_GCGC'y
Cuts at: 632 696 1147 2287 2535 3765 4536 632
Size: 64 451 1140 248 1230 771 949
Fragments arranged by size:
1230 1140 949 771 451 248 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 322 354 2695 2725 2727 2757 322
Size: 32 2341 30 2 30 2418
Fragments arranged by size:
2418 2341 32 30 30 2
HincII GTy'rAC
Cuts at: 1892 1892
Size: 4853
HindIII A'AGCT_T
Cuts at: 1335 1335
Size: 4853
KasI G'GCGC_C
Cuts at: 692 2283 2531 692
Size: 1591 248 3014
Fragments arranged by size:
3014 1591 248
KpnI G_GTAC'C
Cuts at: 1873 1873
Size: 4853
MluI A'CGCG_T
Cuts at: 4286 4286
Size: 4853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3612 3796 3612
Size: 184 4669
MscI TGG'CCA
Cuts at: 139 2614 139
Size: 2475 2378
MslI CAynn'nnrTG
Cuts at: 2842 2969 2842
Size: 127 4726
MspA1I CmG'CkG
Cuts at: 279 714 1758 1964 2278 2638 3422 3667 279
Size: 435 1044 206 314 360 784 245 1465
Fragments arranged by size:
1465 1044 784 435 360 314 245 206
NaeI GCC'GGC
Cuts at: 1802 3034 1802
Size: 1232 3621
NarI GG'CG_CC
Cuts at: 693 2284 2532 693
Size: 1591 248 3014
Fragments arranged by size:
3014 1591 248
NgoMIV G'CCGG_C
Cuts at: 1800 3032 1800
Size: 1232 3621
NspI r_CATG'y
Cuts at: 1906 2406 4009 1906
Size: 500 1603 2750
Fragments arranged by size:
2750 1603 500
PasI CC'CwG_GG
Cuts at: 1103 1646 1103
Size: 543 4310
PciI A'CATG_T
Cuts at: 4005 4005
Size: 4853
PfoI T'CCnGG_A
Cuts at: 614 614
Size: 4853
PmeI GTTT'AAAC
Cuts at: 1852 1852
Size: 4853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 4821
PpuMI rG'GwC_Cy
Cuts at: 126 150 1005 126
Size: 24 855 3974
Fragments arranged by size:
3974 855 24
PstI C_TGCA'G
Cuts at: 125 437 767 959 1076 1355 1555 1900 2585
Size: 312 330 192 117 279 200 345 685
Cuts at: 2585 125
Size: 2393
Fragments arranged by size:
2393 685 345 330 312 279 200 192
117
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4853
PvuII CAG'CTG
Cuts at: 1758 2278 2638 1758
Size: 520 360 3973
Fragments arranged by size:
3973 520 360
RsrII CG'GwC_CG
Cuts at: 3048 3048
Size: 4853
SacI G_AGCT'C
Cuts at: 650 1214 1867 650
Size: 564 653 3636
Fragments arranged by size:
3636 653 564
SacII CC_GC'GG
Cuts at: 280 280
Size: 4853
SalI G'TCGA_C
Cuts at: 1890 1890
Size: 4853
SapI GCTCTTCn'nnn_
Cuts at: 397 787 2876 3086 397
Size: 390 2089 210 2164
Fragments arranged by size:
2164 2089 390 210
SbfI CC_TGCA'GG
Cuts at: 1076 1900 1076
Size: 824 4029
ScaI AGT'ACT
Cuts at: 4370 4370
Size: 4853
SexAI A'CCwGG_T
Cuts at: 895 1219 895
Size: 324 4529
SfcI C'TryA_G
Cuts at: 33 121 433 763 955 1072 1351 1551 1694
Size: 88 312 330 192 117 279 200 143
Cuts at: 1694 1896 2581 3549 3740 33
Size: 202 685 968 191 1146
Fragments arranged by size:
1146 968 685 330 312 279 202 200
192 191 143 117 88
SfoI GGC'GCC
Cuts at: 694 2285 2533 694
Size: 1591 248 3014
Fragments arranged by size:
3014 1591 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4853
SmaI CCC'GGG
Cuts at: 1446 1875 1446
Size: 429 4424
SmlI C'TyrA_G
Cuts at: 187 1540 2008 2923 3637 3899 187
Size: 1353 468 915 714 262 1141
Fragments arranged by size:
1353 1141 915 714 468 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4853
SphI G_CATG'C
Cuts at: 1906 2406 1906
Size: 500 4353
SrfI GCCC'GGGC
Cuts at: 1446 1446
Size: 4853
StyI C'CwwG_G
Cuts at: 470 1591 1987 2341 470
Size: 1121 396 354 2982
Fragments arranged by size:
2982 1121 396 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 847 2436 2569 3028 3237 847
Size: 1589 133 459 209 2463
Fragments arranged by size:
2463 1589 459 209 133
TatI w'GTAC_w
Cuts at: 608 640 1385 4368 608
Size: 32 745 2983 1093
Fragments arranged by size:
2983 1093 745 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1572 1604 1700 1732 4370 4402 1572
Size: 32 96 32 2638 32 2023
Fragments arranged by size:
2638 2023 96 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2650 2650
Size: 4853
XbaI T'CTAG_A
Cuts at: 1884 1884
Size: 4853
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 214 1044 2348 214
Size: 830 1304 2719
Fragments arranged by size:
2719 1304 830
XmaI C'CCGG_G
Cuts at: 1444 1873 1444
Size: 429 4424
XmnI GAAnn'nnTTC
Cuts at: 392 392
Size: 4853
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BglI BmtI BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SanDI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI ZraI