Restriction map for
pF1KA1189
(Circular) (Six-base) MAPSORT of: pF1KA1189.seq Check: 1020 from: 1 to: 3965
pF1KA1189 3965 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1003 1003
Size: 3965
Acc65I G'GTAC_C
Cuts at: 981 981
Size: 3965
AclI AA'CG_TT
Cuts at: 3805 3805
Size: 3965
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1790 2222 2575 3237 1790
Size: 432 353 662 2518
Fragments arranged by size:
2518 662 432 353
AflIII A'CryG_T
Cuts at: 614 1317 3117 3398 614
Size: 703 1800 281 1181
Fragments arranged by size:
1800 1181 703 281
AlwNI CAG_nnn'CTG
Cuts at: 1270 2708 1270
Size: 1438 2527
ApaLI G'TGCA_C
Cuts at: 260 2803 260
Size: 2543 1422
ApoI r'AATT_y
Cuts at: 721 937 969 3318 3574 721
Size: 216 32 2349 256 1112
Fragments arranged by size:
2349 1112 256 216 32
AseI AT'TA_AT
Cuts at: 20 20
Size: 3965
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
AvaI C'yCGr_G
Cuts at: 985 985
Size: 3965
BamHI G'GATC_C
Cuts at: 990 990
Size: 3965
BanI G'GyrC_C
Cuts at: 981 1395 1643 1678 981
Size: 414 248 35 3268
Fragments arranged by size:
3268 414 248 35
BanII G_rGCy'C
Cuts at: 979 2009 979
Size: 1030 2935
BbeI G_GCGC'C
Cuts at: 1399 1647 1399
Size: 248 3717
BbsI GAAGACnn'nnnn_
Cuts at: 498 498
Size: 3965
BciVI GTATCCnnnnn_n'
Cuts at: 1863 2919 3965 1863
Size: 1056 1046 1863
Fragments arranged by size:
1863 1056 1046
BclI T'GATC_A
Cuts at: 1485 1485
Size: 3965
BfrBI ATG'CAT
Cuts at: 954 954
Size: 3965
BglII A'GATC_T
Cuts at: 393 1480 3517 393
Size: 1087 2037 841
Fragments arranged by size:
2037 1087 841
BlpI GC'TnA_GC
Cuts at: 1077 1077
Size: 3965
Bme1580I G_kGCm'C
Cuts at: 264 1590 1683 2807 264
Size: 1326 93 1124 1422
Fragments arranged by size:
1422 1326 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 242 1177 1258 1593 3737 242
Size: 935 81 335 2144 470
Fragments arranged by size:
2144 935 470 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 3965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 843 843
Size: 3965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 2020 2734 3032 1141
Size: 879 714 298 2074
Fragments arranged by size:
2074 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3669 3669
Size: 3965
BsaAI yAC'GTr
Cuts at: 1318 1318
Size: 3965
BsaBI GATnn'nnATC
Cuts at: 6 1479 3516 6
Size: 1473 2037 455
Fragments arranged by size:
2037 1473 455
BsaHI Gr'CG_yC
Cuts at: 1396 1644 2346 3842 1396
Size: 248 702 1496 1519
Fragments arranged by size:
1519 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1154 1675 2353 2764 2911 3185 1154
Size: 521 678 411 147 274 1934
Fragments arranged by size:
1934 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 652 652
Size: 3965
BseYI C'CCAG_C
Cuts at: 585 1390 2148 2813 3221 585
Size: 805 758 665 408 1329
Fragments arranged by size:
1329 805 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1507 1553 2783 104
Size: 1403 46 1230 1286
Fragments arranged by size:
1403 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 264 979 1757 1947 2807 264
Size: 715 778 190 860 1422
Fragments arranged by size:
1422 860 778 715 190
Bsp1286I G_dGCh'C
Cuts at: 264 979 1590 1683 1757 1947 2009 2807 264
Size: 715 611 93 74 190 62 798 1422
Fragments arranged by size:
1422 798 715 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2397 2397
Size: 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1015 1229 1531 1912 1015
Size: 214 302 381 3068
Fragments arranged by size:
3068 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2257 2311 3795 3960 50
Size: 2207 54 1484 165 55
Fragments arranged by size:
2207 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1877 1877
Size: 3965
BsrFI r'CCGG_y
Cuts at: 1963 2144 1963
Size: 181 3784
BssSI C'ACGA_G
Cuts at: 2236 2944 2236
Size: 708 3257
BstAPI GCAn_nnn'nTGC
Cuts at: 1342 1342
Size: 3965
BstBI TT'CG_AA
Cuts at: 594 2326 594
Size: 1732 2233
BstEII G'GTnAC_C
Cuts at: 559 559
Size: 3965
BstYI r'GATC_y
Cuts at: 1 393 990 1457 1480 1815 2061 2367 2379
Size: 392 597 467 23 335 246 306 12
Cuts at: 2379 2465 3517 1
Size: 86 1052 449
Fragments arranged by size:
1052 597 467 449 392 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 837 837
Size: 3965
BtgI C'CryG_G
Cuts at: 326 326
Size: 3965
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1915 2096 2259 1915
Size: 181 163 3621
Fragments arranged by size:
3621 181 163
BtsI GCAGTG_nn'
Cuts at: 801 1208 801
Size: 407 3558
DraI TTT'AAA
Cuts at: 756 964 756
Size: 208 3757
DrdI GACnn_nn'nnGTC
Cuts at: 1671 3015 1671
Size: 1344 2621
EaeI y'GGCC_r
Cuts at: 1550 1724 2115 2142 3487 1550
Size: 174 391 27 1345 2028
Fragments arranged by size:
2028 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1550 1550
Size: 3965
EarI CTCTTCn'nnn_
Cuts at: 353 734 841 1988 2198 353
Size: 381 107 1147 210 2120
Fragments arranged by size:
2120 1147 381 210 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2173 2915 3061 3775 2173
Size: 742 146 714 2363
Fragments arranged by size:
2363 742 714 146
EcoICRI GAG'CTC
Cuts at: 977 977
Size: 3965
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 843 1790 2222 2575 3237 843
Size: 947 432 353 662 1571
Fragments arranged by size:
1571 947 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1104 1104
Size: 3965
EcoRI G'AATT_C
Cuts at: 937 969 937
Size: 32 3933
EcoRV GAT'ATC
Cuts at: 855 855
Size: 3965
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1984 1984
Size: 3965
FspI TGC'GCA
Cuts at: 1746 1746
Size: 3965
HaeII r_GCGC'y
Cuts at: 1399 1647 2877 3648 1399
Size: 248 1230 771 1716
Fragments arranged by size:
1716 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1807 1837 1839 1869 1807
Size: 30 2 30 3903
Fragments arranged by size:
3903 30 30 2
HincII GTy'rAC
Cuts at: 175 1004 175
Size: 829 3136
KasI G'GCGC_C
Cuts at: 1395 1643 1395
Size: 248 3717
KpnI G_GTAC'C
Cuts at: 985 985
Size: 3965
MluI A'CGCG_T
Cuts at: 3398 3398
Size: 3965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2724 2908 2724
Size: 184 3781
MscI TGG'CCA
Cuts at: 1726 1726
Size: 3965
MslI CAynn'nnrTG
Cuts at: 1954 2081 1954
Size: 127 3838
MspA1I CmG'CkG
Cuts at: 571 1076 1390 1750 2534 2779 571
Size: 505 314 360 784 245 1757
Fragments arranged by size:
1757 784 505 360 314 245
NaeI GCC'GGC
Cuts at: 2146 2146
Size: 3965
NarI GG'CG_CC
Cuts at: 1396 1644 1396
Size: 248 3717
NcoI C'CATG_G
Cuts at: 326 326
Size: 3965
NgoMIV G'CCGG_C
Cuts at: 2144 2144
Size: 3965
NsiI A_TGCA'T
Cuts at: 956 956
Size: 3965
NspI r_CATG'y
Cuts at: 312 618 1018 1518 3121 312
Size: 306 400 500 1603 1156
Fragments arranged by size:
1603 1156 500 400 306
PciI A'CATG_T
Cuts at: 614 3117 614
Size: 2503 1462
PmeI GTTT'AAAC
Cuts at: 964 964
Size: 3965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2411 2443 2411
Size: 32 3933
PstI C_TGCA'G
Cuts at: 1012 1697 1012
Size: 685 3280
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3965
PvuII CAG'CTG
Cuts at: 571 1390 1750 571
Size: 819 360 2786
Fragments arranged by size:
2786 819 360
RsrII CG'GwC_CG
Cuts at: 2160 2160
Size: 3965
SacI G_AGCT'C
Cuts at: 979 979
Size: 3965
SalI G'TCGA_C
Cuts at: 1002 1002
Size: 3965
SapI GCTCTTCn'nnn_
Cuts at: 1988 2198 1988
Size: 210 3755
SbfI CC_TGCA'GG
Cuts at: 1012 1012
Size: 3965
ScaI AGT'ACT
Cuts at: 3482 3482
Size: 3965
SfcI C'TryA_G
Cuts at: 33 234 1008 1693 2661 2852 33
Size: 201 774 685 968 191 1146
Fragments arranged by size:
1146 968 774 685 201 191
SfoI GGC'GCC
Cuts at: 1397 1645 1397
Size: 248 3717
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
SmaI CCC'GGG
Cuts at: 987 987
Size: 3965
SmlI C'TyrA_G
Cuts at: 1120 2035 2749 3011 1120
Size: 915 714 262 2074
Fragments arranged by size:
2074 915 714 262
SpeI A'CTAG_T
Cuts at: 67 412 67
Size: 345 3620
SphI G_CATG'C
Cuts at: 1018 1518 1018
Size: 500 3465
StyI C'CwwG_G
Cuts at: 326 1099 1453 326
Size: 773 354 2838
Fragments arranged by size:
2838 773 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1548 1681 2140 2349 1548
Size: 133 459 209 3164
Fragments arranged by size:
3164 459 209 133
TatI w'GTAC_w
Cuts at: 3480 3480
Size: 3965
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 834 866 3482 3514 834
Size: 32 2616 32 1285
Fragments arranged by size:
2616 1285 32 32
Tth111I GACn'n_nGTC
Cuts at: 1762 1762
Size: 3965
XbaI T'CTAG_A
Cuts at: 996 996
Size: 3965
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1460 1460
Size: 3965
XmaI C'CCGG_G
Cuts at: 985 985
Size: 3965
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstXI BstZ17I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI