Restriction map for
pF1KA1190
(Circular) (Six-base) MAPSORT of: pF1KA1190.seq Check: 1866 from: 1 to: 5354
pF1KA1190 5354 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1762 2291 1762
Size: 529 4825
AccI GT'mk_AC
Cuts at: 2392 2392
Size: 5354
Acc65I G'GTAC_C
Cuts at: 1273 1454 2370 1273
Size: 181 916 4257
Fragments arranged by size:
4257 916 181
AclI AA'CG_TT
Cuts at: 2348 5194 2348
Size: 2846 2508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1307 3179 3611 3964 4626 1307
Size: 1872 432 353 662 2035
Fragments arranged by size:
2035 1872 662 432 353
AfeI AGC'GCT
Cuts at: 1519 1858 2110 1519
Size: 339 252 4763
Fragments arranged by size:
4763 339 252
AflIII A'CryG_T
Cuts at: 845 1588 2096 2706 4506 4787 845
Size: 743 508 610 1800 281 1412
Fragments arranged by size:
1800 1412 743 610 508 281
AleI CACnn'nnGTG
Cuts at: 203 203
Size: 5354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1742 1742
Size: 5354
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1816 1848 1816
Size: 32 5322
AlwNI CAG_nnn'CTG
Cuts at: 625 643 840 1369 2265 2659 4097 625
Size: 18 197 529 896 394 1438 1882
Fragments arranged by size:
1882 1438 896 529 394 197 18
ApaI G_GGCC'C
Cuts at: 586 716 586
Size: 130 5224
ApaLI G'TGCA_C
Cuts at: 1642 1801 4192 1642
Size: 159 2391 2804
Fragments arranged by size:
2804 2391 159
ApoI r'AATT_y
Cuts at: 2358 4707 4963 2358
Size: 2349 256 2749
Fragments arranged by size:
2749 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5354
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
AvaI C'yCGr_G
Cuts at: 1250 1352 2247 2374 1250
Size: 102 895 127 4230
Fragments arranged by size:
4230 895 127 102
BamHI G'GATC_C
Cuts at: 2379 2379
Size: 5354
BanI G'GyrC_C
Cuts at: 171 294 778 874 1265 1273 1454 2054 2370
Size: 123 484 96 391 8 181 600 316
Cuts at: 2370 2784 3032 3067 171
Size: 414 248 35 2458
Fragments arranged by size:
2458 600 484 414 391 316 248 181
123 96 35 8
BanII G_rGCy'C
Cuts at: 586 716 1141 2368 3398 586
Size: 130 425 1227 1030 2542
Fragments arranged by size:
2542 1227 1030 425 130
BbeI G_GCGC'C
Cuts at: 1269 2788 3036 1269
Size: 1519 248 3587
Fragments arranged by size:
3587 1519 248
BbsI GAAGACnn'nnnn_
Cuts at: 1984 1984
Size: 5354
BciVI GTATCCnnnnn_n'
Cuts at: 3252 4308 5354 3252
Size: 1056 1046 3252
Fragments arranged by size:
3252 1056 1046
BclI T'GATC_A
Cuts at: 2874 2874
Size: 5354
BglI GCCn_nnn'nGGC
Cuts at: 487 487
Size: 5354
BglII A'GATC_T
Cuts at: 566 1496 1676 2012 2869 4906 566
Size: 930 180 336 857 2037 1014
Fragments arranged by size:
2037 1014 930 857 336 180
BlpI GC'TnA_GC
Cuts at: 376 2466 376
Size: 2090 3264
Bme1580I G_kGCm'C
Cuts at: 176 469 586 716 1073 1432 1646 1805 2979
Size: 293 117 130 357 359 214 159 1174
Cuts at: 2979 3072 4196 176
Size: 93 1124 1334
Fragments arranged by size:
1334 1174 1124 359 357 293 214 159
130 117 93
BmgBI CAC'GTC
Cuts at: 156 2097 156
Size: 1941 3413
BmrI ACTGGGnnnn_n'
Cuts at: 2566 2647 2982 5126 2566
Size: 81 335 2144 2794
Fragments arranged by size:
2794 2144 335 81
BmtI G_CTAG'C
Cuts at: 214 214
Size: 5354
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3721 3721
Size: 5354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 488 757 1352 1417 1480 1549 304
Size: 184 269 595 65 63 69 4109
Fragments arranged by size:
4109 595 269 184 69 65 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1780 2116 2530 3409 4123 4421 1780
Size: 336 414 879 714 298 2713
Fragments arranged by size:
2713 879 714 414 336 298
BsaI GGTCTCn'nnnn_
Cuts at: 1753 5058 1753
Size: 3305 2049
BsaAI yAC'GTr
Cuts at: 846 1710 2707 846
Size: 864 997 3493
Fragments arranged by size:
3493 997 864
BsaBI GATnn'nnATC
Cuts at: 6 2868 4905 6
Size: 2862 2037 455
Fragments arranged by size:
2862 2037 455
BsaHI Gr'CG_yC
Cuts at: 437 1266 2186 2785 3033 3735 5231 437
Size: 829 920 599 248 702 1496 560
Fragments arranged by size:
1496 920 829 702 599 560 248
BsaWI w'CCGG_w
Cuts at: 2543 3064 3742 4153 4300 4574 2543
Size: 521 678 411 147 274 3323
Fragments arranged by size:
3323 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1816 1846 1816
Size: 30 5324
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 694 715 955 958 961 973 976 979 982
Size: 21 240 3 3 12 3 3 3
Cuts at: 982 1024 1027 1030 1033 1072 1075 1108 1111
Size: 42 3 3 3 39 3 33 3
Cuts at: 1111 1120 1159 1162 1165 1174 1414 694
Size: 9 39 3 3 9 240 4634
Fragments arranged by size:
4634 240 240 42 39 39 33 21
12 9 9 3 3 3 3 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 176 482 662 717 888 1171 1294 1377 2216
Size: 306 180 55 171 283 123 83 839
Cuts at: 2216 2779 3537 4202 4610 176
Size: 563 758 665 408 920
Fragments arranged by size:
920 839 758 665 563 408 306 283
180 171 123 83 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 483 826 1531 483
Size: 343 705 4306
Fragments arranged by size:
4306 705 343
BsiEI CG_ry'CG
Cuts at: 104 2896 2942 4172 104
Size: 2792 46 1230 1286
Fragments arranged by size:
2792 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 377 1141 1646 1805 1973 2368 3146 3336 4196
Size: 764 505 159 168 395 778 190 860
Cuts at: 4196 377
Size: 1535
Fragments arranged by size:
1535 860 778 764 505 395 190 168
159
Bsp1286I G_dGCh'C
Cuts at: 176 377 469 586 716 1073 1141 1432 1646
Size: 201 92 117 130 357 68 291 214
Cuts at: 1646 1805 1973 2368 2979 3072 3146 3336 3398
Size: 159 168 395 611 93 74 190 62
Cuts at: 3398 4196 176
Size: 798 1334
Fragments arranged by size:
1334 798 611 395 357 291 214 201
190 168 159 130 117 93 92 74
68 62
BspHI T'CATG_A
Cuts at: 3786 3786
Size: 5354
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1762 1771 2291 2404 2618 2920 3301 1762
Size: 9 520 113 214 302 381 3815
Fragments arranged by size:
3815 520 381 302 214 113 9
BsrBI CCG'CTC
Cuts at: 50 443 2301 3646 3700 5184 5349 50
Size: 393 1858 1345 54 1484 165 55
Fragments arranged by size:
1858 1484 1345 393 165 55 54
BsrDI GCAATG_nn'
Cuts at: 388 1857 3266 388
Size: 1469 1409 2476
Fragments arranged by size:
2476 1469 1409
BsrFI r'CCGG_y
Cuts at: 486 706 2036 2057 2176 3352 3533 486
Size: 220 1330 21 119 1176 181 2307
Fragments arranged by size:
2307 1330 1176 220 181 119 21
BssSI C'ACGA_G
Cuts at: 367 1433 3625 4333 367
Size: 1066 2192 708 1388
Fragments arranged by size:
2192 1388 1066 708
BstAPI GCAn_nnn'nTGC
Cuts at: 625 1809 2235 2731 625
Size: 1184 426 496 3248
Fragments arranged by size:
3248 1184 496 426
BstBI TT'CG_AA
Cuts at: 3715 3715
Size: 5354
BstXI CCAn_nnnn'nTGG
Cuts at: 345 345
Size: 5354
BstYI r'GATC_y
Cuts at: 1 314 566 1496 1676 2012 2379 2846 2869
Size: 313 252 930 180 336 367 467 23
Cuts at: 2869 3204 3450 3756 3768 3854 4906 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 930 467 449 367 336 335 313
306 252 246 180 86 23 12
BtgI C'CryG_G
Cuts at: 107 851 896 1189 1700 107
Size: 744 45 293 511 3761
Fragments arranged by size:
3761 744 511 293 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 393 3304 3485 3648 393
Size: 2911 181 163 2099
Fragments arranged by size:
2911 2099 181 163
BtsI GCAGTG_nn'
Cuts at: 838 1005 1807 2233 2312 2597 838
Size: 167 802 426 79 285 3595
Fragments arranged by size:
3595 802 426 285 167 79
DraI TTT'AAA
Cuts at: 2353 2353
Size: 5354
DraIII CAC_nnn'GTG
Cuts at: 301 372 301
Size: 71 5283
DrdI GACnn_nn'nnGTC
Cuts at: 3060 4404 3060
Size: 1344 4010
EaeI y'GGCC_r
Cuts at: 356 1238 1905 2103 2174 2939 3113 3504 3531
Size: 882 667 198 71 765 174 391 27
Cuts at: 3531 4876 356
Size: 1345 834
Fragments arranged by size:
1345 882 834 765 667 391 198 174
71 27
EagI C'GGCC_G
Cuts at: 2939 2939
Size: 5354
EarI CTCTTCn'nnn_
Cuts at: 139 247 743 755 971 977 1019 1040 1049
Size: 108 496 12 216 6 42 21 9
Cuts at: 1049 1079 1085 1097 1306 2308 3377 3587 139
Size: 30 6 12 209 1002 1069 210 1906
Fragments arranged by size:
1906 1069 1002 496 216 210 209 108
42 30 21 12 12 9 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3562 4304 4450 5164 3562
Size: 742 146 714 3752
Fragments arranged by size:
3752 742 714 146
EcoICRI GAG'CTC
Cuts at: 1139 2366 1139
Size: 1227 4127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 488 757 1307 1352 1417 1480 1549 3179
Size: 184 269 550 45 65 63 69 1630
Cuts at: 3179 3611 3964 4626 304
Size: 432 353 662 1032
Fragments arranged by size:
1630 1032 662 550 432 353 269 184
69 65 63 45
EcoO109I rG'GnC_Cy
Cuts at: 582 583 713 1114 2493 582
Size: 1 130 401 1379 3443
Fragments arranged by size:
3443 1379 401 130 1
EcoRI G'AATT_C
Cuts at: 2358 2358
Size: 5354
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1977 3373 1977
Size: 1396 3958
FspI TGC'GCA
Cuts at: 3135 3135
Size: 5354
HaeII r_GCGC'y
Cuts at: 132 383 1269 1521 1860 2112 2788 3036 4266
Size: 251 886 252 339 252 676 248 1230
Cuts at: 4266 5037 132
Size: 771 449
Fragments arranged by size:
1230 886 771 676 449 339 252 252
251 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 427 459 580 612 3196 3226 3228 3258 427
Size: 32 121 32 2584 30 2 30 2523
Fragments arranged by size:
2584 2523 121 32 32 30 30 2
HincII GTy'rAC
Cuts at: 351 2393 351
Size: 2042 3312
HindIII A'AGCT_T
Cuts at: 1598 1609 1598
Size: 11 5343
KasI G'GCGC_C
Cuts at: 1265 2784 3032 1265
Size: 1519 248 3587
Fragments arranged by size:
3587 1519 248
KpnI G_GTAC'C
Cuts at: 1277 1458 2374 1277
Size: 181 916 4257
Fragments arranged by size:
4257 916 181
MluI A'CGCG_T
Cuts at: 4787 4787
Size: 5354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 734 2147 4113 4297 734
Size: 1413 1966 184 1791
Fragments arranged by size:
1966 1791 1413 184
MscI TGG'CCA
Cuts at: 1240 1907 2105 3115 1240
Size: 667 198 1010 3479
Fragments arranged by size:
3479 1010 667 198
MslI CAynn'nnrTG
Cuts at: 203 1472 1474 3343 3470 203
Size: 1269 2 1869 127 2087
Fragments arranged by size:
2087 1869 1269 127 2
MspA1I CmG'CkG
Cuts at: 261 497 508 1451 1878 2194 2465 2779 3139
Size: 236 11 943 427 316 271 314 360
Cuts at: 3139 3923 4168 261
Size: 784 245 1447
Fragments arranged by size:
1447 943 784 427 360 316 314 271
245 236 11
NaeI GCC'GGC
Cuts at: 488 2038 2178 3535 488
Size: 1550 140 1357 2307
Fragments arranged by size:
2307 1550 1357 140
NarI GG'CG_CC
Cuts at: 1266 2785 3033 1266
Size: 1519 248 3587
Fragments arranged by size:
3587 1519 248
NcoI C'CATG_G
Cuts at: 107 1700 107
Size: 1593 3761
NgoMIV G'CCGG_C
Cuts at: 486 2036 2176 3533 486
Size: 1550 140 1357 2307
Fragments arranged by size:
2307 1550 1357 140
NheI G'CTAG_C
Cuts at: 210 210
Size: 5354
NspI r_CATG'y
Cuts at: 1494 1592 1746 1791 2130 2407 2907 4510 1494
Size: 98 154 45 339 277 500 1603 2338
Fragments arranged by size:
2338 1603 500 339 277 154 98 45
PciI A'CATG_T
Cuts at: 1588 4506 1588
Size: 2918 2436
PflMI CCAn_nnn'nTGG
Cuts at: 1330 2172 1330
Size: 842 4512
PmeI GTTT'AAAC
Cuts at: 2353 2353
Size: 5354
PmlI CAC'GTG
Cuts at: 846 1710 846
Size: 864 4490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1648 1675 1680 1707 1816 1848 3800 3832 1648
Size: 27 5 27 109 32 1952 32 3170
Fragments arranged by size:
3170 1952 109 32 32 27 27 5
PspOMI G'GGCC_C
Cuts at: 582 712 582
Size: 130 5224
PstI C_TGCA'G
Cuts at: 266 311 398 926 1814 2401 3086 266
Size: 45 87 528 888 587 685 2534
Fragments arranged by size:
2534 888 685 587 528 87 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5354
PvuII CAG'CTG
Cuts at: 261 497 508 1878 2779 3139 261
Size: 236 11 1370 901 360 2476
Fragments arranged by size:
2476 1370 901 360 236 11
RsrII CG'GwC_CG
Cuts at: 3549 3549
Size: 5354
SacI G_AGCT'C
Cuts at: 1141 2368 1141
Size: 1227 4127
SalI G'TCGA_C
Cuts at: 2391 2391
Size: 5354
SapI GCTCTTCn'nnn_
Cuts at: 755 2308 3377 3587 755
Size: 1553 1069 210 2522
Fragments arranged by size:
2522 1553 1069 210
SbfI CC_TGCA'GG
Cuts at: 2401 2401
Size: 5354
ScaI AGT'ACT
Cuts at: 1960 4871 1960
Size: 2911 2443
SexAI A'CCwGG_T
Cuts at: 297 456 297
Size: 159 5195
SfcI C'TryA_G
Cuts at: 33 219 262 307 394 783 922 1810 2397
Size: 186 43 45 87 389 139 888 587
Cuts at: 2397 3082 4050 4241 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 888 685 587 389 191 186
139 87 45 43
SfoI GGC'GCC
Cuts at: 1267 2786 3034 1267
Size: 1519 248 3587
Fragments arranged by size:
3587 1519 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
SgrAI Cr'CCGG_yG
Cuts at: 2057 2057
Size: 5354
SmaI CCC'GGG
Cuts at: 1252 2249 2376 1252
Size: 997 127 4230
Fragments arranged by size:
4230 997 127
SmlI C'TyrA_G
Cuts at: 1795 2131 2509 3424 4138 4400 1795
Size: 336 378 915 714 262 2749
Fragments arranged by size:
2749 915 714 378 336 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5354
SphI G_CATG'C
Cuts at: 1494 2407 2907 1494
Size: 913 500 3941
Fragments arranged by size:
3941 913 500
StuI AGG'CCT
Cuts at: 557 2204 557
Size: 1647 3707
StyI C'CwwG_G
Cuts at: 107 1385 1700 1736 2488 2842 107
Size: 1278 315 36 752 354 2619
Fragments arranged by size:
2619 1278 752 354 315 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 262 2937 3070 3529 3738 262
Size: 2675 133 459 209 1878
Fragments arranged by size:
2675 1878 459 209 133
TatI w'GTAC_w
Cuts at: 1865 1958 4869 1865
Size: 93 2911 2350
Fragments arranged by size:
2911 2350 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 615 647 858 890 4871 4903 615
Size: 32 211 32 3981 32 1066
Fragments arranged by size:
3981 1066 211 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2185 3151 2185
Size: 966 4388
XbaI T'CTAG_A
Cuts at: 2385 2385
Size: 5354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 900 1374 1392 2849 900
Size: 474 18 1457 3405
Fragments arranged by size:
3405 1457 474 18
XmaI C'CCGG_G
Cuts at: 1250 2247 2374 1250
Size: 997 127 4230
Fragments arranged by size:
4230 997 127
XmnI GAAnn'nnTTC
Cuts at: 1663 1690 1831 2152 1663
Size: 27 141 321 4865
Fragments arranged by size:
4865 321 141 27
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PflMI PmeI PmlI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AscI AvrII BaeI BbvCI
BcgI BfrBI Bpu10I BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NotI NruI NsiI
PacI PasI PfoI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SnaBI SrfI SspI SwaI XhoI
ZraI