Restriction map for
pF1KA1198
(Circular) (Six-base) MAPSORT of: pF1KA1198.seq Check: 6882 from: 1 to: 4700
pF1KA1198 4700 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1620 1683 1738 1620
Size: 63 55 4582
Fragments arranged by size:
4582 63 55
Acc65I G'GTAC_C
Cuts at: 1716 1716
Size: 4700
AclI AA'CG_TT
Cuts at: 4540 4540
Size: 4700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1035 1306 1464 1623 2525 2957 3704 3972 1035
Size: 271 158 159 902 432 747 268 1763
Fragments arranged by size:
1763 902 747 432 271 268 159 158
AflIII A'CryG_T
Cuts at: 1161 1195 2052 3156 4133 1161
Size: 34 857 1104 977 1728
Fragments arranged by size:
1728 1104 977 857 34
AgeI A'CCGG_T
Cuts at: 3088 3088
Size: 4700
AleI CACnn'nnGTG
Cuts at: 1516 1516
Size: 4700
AlwNI CAG_nnn'CTG
Cuts at: 2005 3572 2005
Size: 1567 3133
ApaLI G'TGCA_C
Cuts at: 1243 1663 3470 1243
Size: 420 1807 2473
Fragments arranged by size:
2473 1807 420
ApoI r'AATT_y
Cuts at: 117 749 833 1566 1589 1704 4053 4309 117
Size: 632 84 733 23 115 2349 256 508
Fragments arranged by size:
2349 733 632 508 256 115 84 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4700
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
AvaI C'yCGr_G
Cuts at: 151 1720 151
Size: 1569 3131
BamHI G'GATC_C
Cuts at: 330 1725 330
Size: 1395 3305
BanI G'GyrC_C
Cuts at: 905 1716 2130 2378 2413 905
Size: 811 414 248 35 3192
Fragments arranged by size:
3192 811 414 248 35
BanII G_rGCy'C
Cuts at: 1714 2744 1714
Size: 1030 3670
BbeI G_GCGC'C
Cuts at: 909 2134 2382 909
Size: 1225 248 3227
Fragments arranged by size:
3227 1225 248
BbsI GAAGACnn'nnnn_
Cuts at: 591 1461 591
Size: 870 3830
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 780 814 90
Size: 34 656 34 3976
Fragments arranged by size:
3976 656 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2598 3359 4700 2598
Size: 761 1341 2598
Fragments arranged by size:
2598 1341 761
BclI T'GATC_A
Cuts at: 2220 2220
Size: 4700
BfrBI ATG'CAT
Cuts at: 606 606
Size: 4700
BglII A'GATC_T
Cuts at: 634 2215 4252 634
Size: 1581 2037 1082
Fragments arranged by size:
2037 1581 1082
BlpI GC'TnA_GC
Cuts at: 1812 1812
Size: 4700
Bme1580I G_kGCm'C
Cuts at: 1247 1667 2325 2418 3474 1247
Size: 420 658 93 1056 2473
Fragments arranged by size:
2473 1056 658 420 93
BmrI ACTGGGnnnn_n'
Cuts at: 1912 1993 2328 4472 1912
Size: 81 335 2144 2140
Fragments arranged by size:
2144 2140 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 4700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 331 711 779 863 947 1031 1115 1535
Size: 126 380 68 84 84 84 84 420
Cuts at: 1535 1619 205
Size: 84 3286
Fragments arranged by size:
3286 420 380 126 84 84 84 84
84 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 1131 1876 2755 3247 3545 304
Size: 827 745 879 492 298 1459
Fragments arranged by size:
1459 879 827 745 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 618 4404 618
Size: 3786 914
BsaAI yAC'GTr
Cuts at: 1064 1164 1662 2053 1064
Size: 100 498 391 3711
Fragments arranged by size:
3711 498 391 100
BsaBI GATnn'nnATC
Cuts at: 6 2214 4251 6
Size: 2208 2037 455
Fragments arranged by size:
2208 2037 455
BsaHI Gr'CG_yC
Cuts at: 906 2131 2379 3081 4577 906
Size: 1225 248 702 1496 1029
Fragments arranged by size:
1496 1225 1029 702 248
BsaWI w'CCGG_w
Cuts at: 1177 1889 2410 3088 3362 3509 3920 1177
Size: 712 521 678 274 147 411 1957
Fragments arranged by size:
1957 712 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 262 292 262
Size: 30 4670
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 328 169
Size: 159 4541
BseYI C'CCAG_C
Cuts at: 2125 2883 3460 3956 2125
Size: 758 577 496 2869
Fragments arranged by size:
2869 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2242 2288 3496 104
Size: 2138 46 1208 1308
Fragments arranged by size:
2138 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1247 1667 1714 2492 2682 3474 1247
Size: 420 47 778 190 792 2473
Fragments arranged by size:
2473 792 778 420 190 47
BsmI GAATG_Cn'
Cuts at: 880 1453 880
Size: 573 4127
Bsp1286I G_dGCh'C
Cuts at: 1247 1667 1714 2325 2418 2492 2682 2744 3474
Size: 420 47 611 93 74 190 62 730
Cuts at: 3474 1247
Size: 2473
Fragments arranged by size:
2473 730 611 420 190 93 74 62
47
BspEI T'CCGG_A
Cuts at: 3920 3920
Size: 4700
BspHI T'CATG_A
Cuts at: 1077 3876 1077
Size: 2799 1901
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1750 1964 2266 2647 1750
Size: 214 302 381 3803
Fragments arranged by size:
3803 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2992 3046 4530 4695 50
Size: 2942 54 1484 165 55
Fragments arranged by size:
2942 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1042 2612 1042
Size: 1570 3130
BsrFI r'CCGG_y
Cuts at: 2698 2879 3088 2698
Size: 181 209 4310
Fragments arranged by size:
4310 209 181
BssSI C'ACGA_G
Cuts at: 2971 3329 2971
Size: 358 4342
BstAPI GCAn_nnn'nTGC
Cuts at: 2077 2077
Size: 4700
BstBI TT'CG_AA
Cuts at: 735 819 1155 1407 1491 3061 735
Size: 84 336 252 84 1570 2374
Fragments arranged by size:
2374 1570 336 252 84 84
BstYI r'GATC_y
Cuts at: 1 330 634 1725 2192 2215 2550 2796 3808
Size: 329 304 1091 467 23 335 246 1012
Cuts at: 3808 3894 3906 4252 1
Size: 86 12 346 449
Fragments arranged by size:
1091 1012 467 449 346 335 329 304
246 86 23 12
BtgI C'CryG_G
Cuts at: 243 243
Size: 4700
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2650 2831 2994 2650
Size: 181 163 4356
Fragments arranged by size:
4356 181 163
BtsI GCAGTG_nn'
Cuts at: 588 893 1546 1943 588
Size: 305 653 397 3345
Fragments arranged by size:
3345 653 397 305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 684 719 1440 1475 684
Size: 35 721 35 3909
Fragments arranged by size:
3909 721 35 35
DraI TTT'AAA
Cuts at: 1565 1699 1565
Size: 134 4566
DraIII CAC_nnn'GTG
Cuts at: 481 481
Size: 4700
DrdI GACnn_nn'nnGTC
Cuts at: 2406 3264 2406
Size: 858 3842
EaeI y'GGCC_r
Cuts at: 336 2285 2459 2850 2877 4222 336
Size: 1949 174 391 27 1345 814
Fragments arranged by size:
1949 1345 814 391 174 27
EagI C'GGCC_G
Cuts at: 2285 2285
Size: 4700
EarI CTCTTCn'nnn_
Cuts at: 905 2723 2933 905
Size: 1818 210 2672
Fragments arranged by size:
2672 1818 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2908 3218 3364 4510 2908
Size: 310 146 1146 3098
Fragments arranged by size:
3098 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1712 1712
Size: 4700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 331 711 779 863 947 1031 1035 1115
Size: 126 380 68 84 84 84 4 80
Cuts at: 1115 1306 1464 1535 1619 1623 2525 2957 3704
Size: 191 158 71 84 4 902 432 747
Cuts at: 3704 3972 205
Size: 268 933
Fragments arranged by size:
933 902 747 432 380 268 191 158
126 84 84 84 84 80 71 68
4 4
EcoO109I rG'GnC_Cy
Cuts at: 1839 1839
Size: 4700
EcoRI G'AATT_C
Cuts at: 117 749 833 1589 1704 117
Size: 632 84 756 115 3113
Fragments arranged by size:
3113 756 632 115 84
EcoRV GAT'ATC
Cuts at: 889 889
Size: 4700
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1405 2719 1405
Size: 1314 3386
FspI TGC'GCA
Cuts at: 112 2481 112
Size: 2369 2331
HaeII r_GCGC'y
Cuts at: 909 2134 2382 3404 4383 909
Size: 1225 248 1022 979 1226
Fragments arranged by size:
1226 1225 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 239 262 271 294 516 548 2542 2572 2574
Size: 23 9 23 222 32 1994 30 2
Cuts at: 2574 2604 239
Size: 30 2335
Fragments arranged by size:
2335 1994 222 32 30 30 23 23
9 2
HincII GTy'rAC
Cuts at: 1739 1739
Size: 4700
HindIII A'AGCT_T
Cuts at: 1550 1550
Size: 4700
KasI G'GCGC_C
Cuts at: 905 2130 2378 905
Size: 1225 248 3227
Fragments arranged by size:
3227 1225 248
KpnI G_GTAC'C
Cuts at: 1720 1720
Size: 4700
MluI A'CGCG_T
Cuts at: 4133 4133
Size: 4700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3371 3555 3371
Size: 184 4516
MscI TGG'CCA
Cuts at: 338 2461 338
Size: 2123 2577
MslI CAynn'nnrTG
Cuts at: 671 690 1516 1530 2689 2816 671
Size: 19 826 14 1159 127 2555
Fragments arranged by size:
2555 1159 826 127 19 14
MspA1I CmG'CkG
Cuts at: 1811 2125 2485 3498 3743 1811
Size: 314 360 1013 245 2768
Fragments arranged by size:
2768 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2881 2881
Size: 4700
NarI GG'CG_CC
Cuts at: 906 2131 2379 906
Size: 1225 248 3227
Fragments arranged by size:
3227 1225 248
NcoI C'CATG_G
Cuts at: 243 243
Size: 4700
NgoMIV G'CCGG_C
Cuts at: 2879 2879
Size: 4700
NsiI A_TGCA'T
Cuts at: 608 608
Size: 4700
NspI r_CATG'y
Cuts at: 1199 1753 2253 3160 1199
Size: 554 500 907 2739
Fragments arranged by size:
2739 907 554 500
PciI A'CATG_T
Cuts at: 1195 3156 1195
Size: 1961 2739
PmeI GTTT'AAAC
Cuts at: 1699 1699
Size: 4700
PmlI CAC'GTG
Cuts at: 1164 1662 1164
Size: 498 4202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 3839
Size: 32 4668
PspXI vC'TCGA_Gb
Cuts at: 151 151
Size: 4700
PstI C_TGCA'G
Cuts at: 583 1747 2432 583
Size: 1164 685 2851
Fragments arranged by size:
2851 1164 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4700
PvuII CAG'CTG
Cuts at: 2125 2485 2125
Size: 360 4340
RsrII CG'GwC_CG
Cuts at: 2895 2895
Size: 4700
SacI G_AGCT'C
Cuts at: 1714 1714
Size: 4700
SalI G'TCGA_C
Cuts at: 1737 1737
Size: 4700
SapI GCTCTTCn'nnn_
Cuts at: 905 2723 2933 905
Size: 1818 210 2672
Fragments arranged by size:
2672 1818 210
SbfI CC_TGCA'GG
Cuts at: 1747 1747
Size: 4700
ScaI AGT'ACT
Cuts at: 4217 4217
Size: 4700
SfcI C'TryA_G
Cuts at: 33 351 579 1743 2428 3421 3612 33
Size: 318 228 1164 685 993 191 1121
Fragments arranged by size:
1164 1121 993 685 318 228 191
SfoI GGC'GCC
Cuts at: 907 2132 2380 907
Size: 1225 248 3227
Fragments arranged by size:
3227 1225 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
SmaI CCC'GGG
Cuts at: 1722 1722
Size: 4700
SmlI C'TyrA_G
Cuts at: 151 283 1146 1855 2770 3262 3524 151
Size: 132 863 709 915 492 262 1327
Fragments arranged by size:
1327 915 863 709 492 262 132
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4700
SphI G_CATG'C
Cuts at: 1753 2253 1753
Size: 500 4200
StyI C'CwwG_G
Cuts at: 243 1834 2188 243
Size: 1591 354 2755
Fragments arranged by size:
2755 1591 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2283 2416 2875 3084 2283
Size: 133 459 209 3899
Fragments arranged by size:
3899 459 209 133
TatI w'GTAC_w
Cuts at: 1578 4215 1578
Size: 2637 2063
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4217 4249 4217
Size: 32 4668
Tth111I GACn'n_nGTC
Cuts at: 2497 2497
Size: 4700
XbaI T'CTAG_A
Cuts at: 1731 1731
Size: 4700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2195 2195
Size: 4700
XhoI C'TCGA_G
Cuts at: 151 151
Size: 4700
XmaI C'CCGG_G
Cuts at: 1720 1720
Size: 4700
XmnI GAAnn'nnTTC
Cuts at: 712 749 880 1048 1337 712
Size: 37 131 168 289 4075
Fragments arranged by size:
4075 289 168 131 37
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PmeI
PmlI PpiI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI EcoNI FseI FspAI HpaI MfeI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PpuMI
PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI ZraI