Restriction map for
pF1KA1204
(Circular) (Six-base) MAPSORT of: pF1KA1204.seq Check: 5884 from: 1 to: 7445
pF1KA1204 7445 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 702 2463 4193 702
Size: 1761 1730 3954
Fragments arranged by size:
3954 1761 1730
AccI GT'mk_AC
Cuts at: 4483 4483
Size: 7445
Acc65I G'GTAC_C
Cuts at: 4461 4461
Size: 7445
AclI AA'CG_TT
Cuts at: 7285 7285
Size: 7445
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 497 567 1423 1820 2158 2593 2747 2893 3052
Size: 70 856 397 338 435 154 146 159
Cuts at: 3052 3111 5270 5702 6449 6717 497
Size: 59 2159 432 747 268 1225
Fragments arranged by size:
2159 1225 856 747 435 432 397 338
268 159 154 146 70 59
AflIII A'CryG_T
Cuts at: 4797 5901 6878 4797
Size: 1104 977 5364
Fragments arranged by size:
5364 1104 977
AgeI A'CCGG_T
Cuts at: 1216 5833 1216
Size: 4617 2828
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 191 223 191
Size: 32 7413
AlwNI CAG_nnn'CTG
Cuts at: 135 1168 1756 1798 2085 2340 3029 3600 3646
Size: 1033 588 42 287 255 689 571 46
Cuts at: 3646 3753 3792 4750 6317 135
Size: 107 39 958 1567 1263
Fragments arranged by size:
1567 1263 1033 958 689 588 571 287
255 107 46 42 39
ApaI G_GGCC'C
Cuts at: 2183 3398 2183
Size: 1215 6230
ApaLI G'TGCA_C
Cuts at: 764 6215 764
Size: 5451 1994
ApoI r'AATT_y
Cuts at: 235 448 1194 2649 3054 4449 6798 7054 235
Size: 213 746 1455 405 1395 2349 256 626
Fragments arranged by size:
2349 1455 1395 746 626 405 256 213
AseI AT'TA_AT
Cuts at: 20 20
Size: 7445
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7445
AvaI C'yCGr_G
Cuts at: 195 1321 2164 3007 4465 195
Size: 1126 843 843 1458 3175
Fragments arranged by size:
3175 1458 1126 843 843
BamHI G'GATC_C
Cuts at: 1425 2268 4470 1425
Size: 843 2202 4400
Fragments arranged by size:
4400 2202 843
BanI G'GyrC_C
Cuts at: 630 949 1258 1849 3078 4461 4875 5123 5158
Size: 319 309 591 1229 1383 414 248 35
Cuts at: 5158 630
Size: 2917
Fragments arranged by size:
2917 1383 1229 591 414 319 309 248
35
BanII G_rGCy'C
Cuts at: 385 440 1532 1574 1894 1996 2114 2183 3398
Size: 55 1092 42 320 102 118 69 1215
Cuts at: 3398 3769 4459 5489 385
Size: 371 690 1030 2341
Fragments arranged by size:
2341 1215 1092 1030 690 371 320 118
102 69 55 42
BbeI G_GCGC'C
Cuts at: 634 1853 4879 5127 634
Size: 1219 3026 248 2952
Fragments arranged by size:
3026 2952 1219 248
BbsI GAAGACnn'nnnn_
Cuts at: 1494 2146 2534 4140 1494
Size: 652 388 1606 4799
Fragments arranged by size:
4799 1606 652 388
BbvCI CC'TCA_GC
Cuts at: 1328 4391 1328
Size: 3063 4382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1558 1592 1635 1669 1558
Size: 34 43 34 7334
Fragments arranged by size:
7334 43 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5343 6104 7445 5343
Size: 761 1341 5343
Fragments arranged by size:
5343 1341 761
BclI T'GATC_A
Cuts at: 4965 4965
Size: 7445
BglI GCCn_nnn'nGGC
Cuts at: 2362 2362
Size: 7445
BglII A'GATC_T
Cuts at: 339 2553 2594 4960 6997 339
Size: 2214 41 2366 2037 787
Fragments arranged by size:
2366 2214 2037 787 41
BlpI GC'TnA_GC
Cuts at: 125 4557 125
Size: 4432 3013
Bme1580I G_kGCm'C
Cuts at: 768 952 2183 3263 3398 5070 5163 6219 768
Size: 184 1231 1080 135 1672 93 1056 1994
Fragments arranged by size:
1994 1672 1231 1080 1056 184 135 93
BmrI ACTGGGnnnn_n'
Cuts at: 2835 3703 4657 4738 5073 7217 2835
Size: 868 954 81 335 2144 3063
Fragments arranged by size:
3063 2144 954 868 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 339 2130 2736 2984 3033 5812 339
Size: 1791 606 248 49 2779 1972
Fragments arranged by size:
2779 1972 1791 606 248 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 799 984 1351 1879 2219 2272 2477 2500
Size: 453 185 367 528 340 53 205 23
Cuts at: 2500 2833 3006 3371 3591 3814 3959 346
Size: 333 173 365 220 223 145 3832
Fragments arranged by size:
3832 528 453 367 365 340 333 223
220 205 185 173 145 53 23
Bpu10I CC'TnA_GC
Cuts at: 307 1328 1376 2330 3768 4391 307
Size: 1021 48 954 1438 623 3361
Fragments arranged by size:
3361 1438 1021 954 623 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 877 991 1798 1908 4621 5500 5992 6290
Size: 26 114 807 110 2713 879 492 298
Cuts at: 6290 851
Size: 2006
Fragments arranged by size:
2713 2006 879 807 492 298 114 110
26
BsaI GGTCTCn'nnnn_
Cuts at: 779 3513 3515 4357 7149 779
Size: 2734 2 842 2792 1075
Fragments arranged by size:
2792 2734 1075 842 2
BsaAI yAC'GTr
Cuts at: 4798 4798
Size: 7445
BsaBI GATnn'nnATC
Cuts at: 6 4959 6996 6
Size: 4953 2037 455
Fragments arranged by size:
4953 2037 455
BsaHI Gr'CG_yC
Cuts at: 631 1850 4876 5124 5826 7322 631
Size: 1219 3026 248 702 1496 754
Fragments arranged by size:
3026 1496 1219 754 702 248
BsaWI w'CCGG_w
Cuts at: 1216 1392 1428 1929 4634 5155 5833 6107 6254
Size: 176 36 501 2705 521 678 274 147
Cuts at: 6254 6665 1216
Size: 411 1996
Fragments arranged by size:
2705 1996 678 521 501 411 274 176
147 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 448 478 1642 1672 3957 3985 3987 4015 448
Size: 30 1164 30 2285 28 2 28 3878
Fragments arranged by size:
3878 2285 1164 30 30 28 28 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 631 1876 1960 2119 2122 2357 2432 2450 2465
Size: 1245 84 159 3 235 75 18 15
Cuts at: 2465 2497 2632 2746 3009 3152 3813 3855 631
Size: 32 135 114 263 143 661 42 4221
Fragments arranged by size:
4221 1245 661 263 235 159 143 135
114 84 75 42 32 18 15 3
BseYI C'CCAG_C
Cuts at: 1731 2801 2947 3201 3325 3814 4870 5628 6205
Size: 1070 146 254 124 489 1056 758 577
Cuts at: 6205 6701 1731
Size: 496 2475
Fragments arranged by size:
2475 1070 1056 758 577 496 489 254
146 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 1653 2326 3352 3926 251
Size: 1402 673 1026 574 3770
Fragments arranged by size:
3770 1402 1026 673 574
BsiEI CG_ry'CG
Cuts at: 104 4987 5033 6241 104
Size: 4883 46 1208 1308
Fragments arranged by size:
4883 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 440 768 1894 2114 2287 3359 3385 3769 4459
Size: 328 1126 220 173 1072 26 384 690
Cuts at: 4459 5237 5427 6219 440
Size: 778 190 792 1666
Fragments arranged by size:
1666 1126 1072 792 778 690 384 328
220 190 173 26
BsmI GAATG_Cn'
Cuts at: 1825 2664 3506 1825
Size: 839 842 5764
Fragments arranged by size:
5764 842 839
BsmBI CGTCTCn'nnnn_
Cuts at: 2724 2724
Size: 7445
Bsp1286I G_dGCh'C
Cuts at: 385 440 768 952 1532 1574 1894 1996 2114
Size: 55 328 184 580 42 320 102 118
Cuts at: 2114 2183 2287 3263 3359 3385 3398 3769 4459
Size: 69 104 976 96 26 13 371 690
Cuts at: 4459 5070 5163 5237 5427 5489 6219 385
Size: 611 93 74 190 62 730 1611
Fragments arranged by size:
1611 976 730 690 611 580 371 328
320 190 184 118 104 102 96 93
74 69 62 55 42 26 13
BspEI T'CCGG_A
Cuts at: 1428 1929 6665 1428
Size: 501 4736 2208
Fragments arranged by size:
4736 2208 501
BspHI T'CATG_A
Cuts at: 803 6621 803
Size: 5818 1627
BspMI ACCTGCnnnn'nnnn_
Cuts at: 702 1762 2463 3495 3852 4004 4193 4495 4709
Size: 1060 701 1032 357 152 189 302 214
Cuts at: 4709 5011 5392 702
Size: 302 381 2755
Fragments arranged by size:
2755 1060 1032 701 381 357 302 302
214 189 152
BsrBI CCG'CTC
Cuts at: 50 1593 1703 5737 5791 7275 7440 50
Size: 1543 110 4034 54 1484 165 55
Fragments arranged by size:
4034 1543 1484 165 110 55 54
BsrDI GCAATG_nn'
Cuts at: 468 681 2795 3449 4030 5357 468
Size: 213 2114 654 581 1327 2556
Fragments arranged by size:
2556 2114 1327 654 581 213
BsrFI r'CCGG_y
Cuts at: 794 1216 1255 1261 5443 5624 5833 794
Size: 422 39 6 4182 181 209 2406
Fragments arranged by size:
4182 2406 422 209 181 39 6
BssSI C'ACGA_G
Cuts at: 5716 6074 5716
Size: 358 7087
BstAPI GCAn_nnn'nTGC
Cuts at: 1631 2340 4822 1631
Size: 709 2482 4254
Fragments arranged by size:
4254 2482 709
BstBI TT'CG_AA
Cuts at: 1477 5806 1477
Size: 4329 3116
BstEII G'GTnAC_C
Cuts at: 2914 2914
Size: 7445
BstXI CCAn_nnnn'nTGG
Cuts at: 846 2250 846
Size: 1404 6041
BstYI r'GATC_y
Cuts at: 1 339 1425 2268 2553 2594 3740 3903 4470
Size: 338 1086 843 285 41 1146 163 567
Cuts at: 4470 4937 4960 5295 5541 6553 6639 6651 6997
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6997 1
Size: 449
Fragments arranged by size:
1146 1086 1012 843 567 467 449 346
338 335 285 246 163 86 41 23
12
Bsu36I CC'TnA_GG
Cuts at: 643 3118 643
Size: 2475 4970
BtgI C'CryG_G
Cuts at: 944 2984 944
Size: 2040 5405
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5395 5576 5739 5395
Size: 181 163 7101
Fragments arranged by size:
7101 181 163
BtsI GCAGTG_nn'
Cuts at: 467 1356 3938 4688 467
Size: 889 2582 750 3224
Fragments arranged by size:
3224 2582 889 750
DraI TTT'AAA
Cuts at: 1904 4444 1904
Size: 2540 4905
DraIII CAC_nnn'GTG
Cuts at: 378 1631 3389 378
Size: 1253 1758 4434
Fragments arranged by size:
4434 1758 1253
DrdI GACnn_nn'nnGTC
Cuts at: 3529 5151 6009 3529
Size: 1622 858 4965
Fragments arranged by size:
4965 1622 858
EaeI y'GGCC_r
Cuts at: 881 1711 2048 4350 5030 5204 5595 5622 6967
Size: 830 337 2302 680 174 391 27 1345
Cuts at: 6967 881
Size: 1359
Fragments arranged by size:
2302 1359 1345 830 680 391 337 174
27
EagI C'GGCC_G
Cuts at: 5030 5030
Size: 7445
EarI CTCTTCn'nnn_
Cuts at: 217 802 961 1522 1670 1715 1912 2723 2923
Size: 585 159 561 148 45 197 811 200
Cuts at: 2923 3071 3466 5468 5678 217
Size: 148 395 2002 210 1984
Fragments arranged by size:
2002 1984 811 585 561 395 210 200
197 159 148 148 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5653 5963 6109 7255 5653
Size: 310 146 1146 5843
Fragments arranged by size:
5843 1146 310 146
EcoICRI GAG'CTC
Cuts at: 438 1892 2112 3767 4457 438
Size: 1454 220 1655 690 3426
Fragments arranged by size:
3426 1655 1454 690 220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 497 567 799 984 1351 1423 1820 1879
Size: 151 70 232 185 367 72 397 59
Cuts at: 1879 2158 2219 2272 2477 2500 2593 2747 2833
Size: 279 61 53 205 23 93 154 86
Cuts at: 2833 2893 3006 3052 3111 3371 3591 3814 3959
Size: 60 113 46 59 260 220 223 145
Cuts at: 3959 5270 5702 6449 6717 346
Size: 1311 432 747 268 1074
Fragments arranged by size:
1311 1074 747 432 397 367 279 268
260 232 223 220 205 185 154 151
145 113 93 86 72 70 61 60
59 59 53 46 23
EcoNI CCTnn'n_nnAGG
Cuts at: 479 1172 1837 2608 3229 479
Size: 693 665 771 621 4695
Fragments arranged by size:
4695 771 693 665 621
EcoO109I rG'GnC_Cy
Cuts at: 539 2179 2180 2337 2904 3122 3395 3588 3597
Size: 1640 1 157 567 218 273 193 9
Cuts at: 3597 3811 3961 4218 4584 539
Size: 214 150 257 366 3400
Fragments arranged by size:
3400 1640 567 366 273 257 218 214
193 157 150 9 1
EcoRI G'AATT_C
Cuts at: 3054 4449 3054
Size: 1395 6050
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 417 2035 5464 417
Size: 1618 3429 2398
Fragments arranged by size:
3429 2398 1618
FspI TGC'GCA
Cuts at: 5226 5226
Size: 7445
HaeII r_GCGC'y
Cuts at: 634 1376 1558 1853 4879 5127 6149 7128 634
Size: 742 182 295 3026 248 1022 979 951
Fragments arranged by size:
3026 1022 979 951 742 295 248 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 168 200 448 480 924 956 1045 1077 1091
Size: 32 248 32 444 32 89 32 14
Cuts at: 1091 1123 1338 1370 1642 1674 1911 1943 1957
Size: 32 215 32 272 32 237 32 14
Cuts at: 1957 1989 2101 2133 2253 2285 2380 2412 2538
Size: 32 112 32 120 32 95 32 126
Cuts at: 2538 2570 3847 3879 4078 4110 5287 5317 5319
Size: 32 1277 32 199 32 1177 30 2
Cuts at: 5319 5349 168
Size: 30 2264
Fragments arranged by size:
2264 1277 1177 444 272 248 237 215
199 126 120 112 95 89 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 30 30 14
14 2
HincII GTy'rAC
Cuts at: 2489 4484 2489
Size: 1995 5450
KasI G'GCGC_C
Cuts at: 630 1849 4875 5123 630
Size: 1219 3026 248 2952
Fragments arranged by size:
3026 2952 1219 248
KpnI G_GTAC'C
Cuts at: 4465 4465
Size: 7445
MluI A'CGCG_T
Cuts at: 6878 6878
Size: 7445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1155 2494 2903 6116 6300 1155
Size: 1339 409 3213 184 2300
Fragments arranged by size:
3213 2300 1339 409 184
MscI TGG'CCA
Cuts at: 883 1713 2050 4352 5206 883
Size: 830 337 2302 854 3122
Fragments arranged by size:
3122 2302 854 830 337
MslI CAynn'nnrTG
Cuts at: 1904 3538 5434 5561 1904
Size: 1634 1896 127 3788
Fragments arranged by size:
3788 1896 1634 127
MspA1I CmG'CkG
Cuts at: 1401 1731 2571 3029 3661 4376 4395 4556 4870
Size: 330 840 458 632 715 19 161 314
Cuts at: 4870 5230 6243 6488 1401
Size: 360 1013 245 2358
Fragments arranged by size:
2358 1013 840 715 632 458 360 330
314 245 161 19
NaeI GCC'GGC
Cuts at: 5626 5626
Size: 7445
NarI GG'CG_CC
Cuts at: 631 1850 4876 5124 631
Size: 1219 3026 248 2952
Fragments arranged by size:
3026 2952 1219 248
NcoI C'CATG_G
Cuts at: 944 944
Size: 7445
NgoMIV G'CCGG_C
Cuts at: 5624 5624
Size: 7445
NspI r_CATG'y
Cuts at: 603 3954 4041 4498 4998 5905 603
Size: 3351 87 457 500 907 2143
Fragments arranged by size:
3351 2143 907 500 457 87
PasI CC'CwG_GG
Cuts at: 1997 2828 1997
Size: 831 6614
PciI A'CATG_T
Cuts at: 5901 5901
Size: 7445
PflMI CCAn_nnn'nTGG
Cuts at: 378 714 2031 3600 378
Size: 336 1317 1569 4223
Fragments arranged by size:
4223 1569 1317 336
PfoI T'CCnGG_A
Cuts at: 362 512 2536 3906 362
Size: 150 2024 1370 3901
Fragments arranged by size:
3901 2024 1370 150
PmeI GTTT'AAAC
Cuts at: 4444 4444
Size: 7445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6584 6616 6584
Size: 32 7413
PpuMI rG'GwC_Cy
Cuts at: 539 3122 3588 3597 3811 3961 4218 539
Size: 2583 466 9 214 150 257 3766
Fragments arranged by size:
3766 2583 466 257 214 150 9
PspOMI G'GGCC_C
Cuts at: 2179 3394 2179
Size: 1215 6230
PstI C_TGCA'G
Cuts at: 1658 1752 1988 4492 5177 1658
Size: 94 236 2504 685 3926
Fragments arranged by size:
3926 2504 685 236 94
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7445
PvuII CAG'CTG
Cuts at: 1401 2571 3029 3661 4376 4870 5230 1401
Size: 1170 458 632 715 494 360 3616
Fragments arranged by size:
3616 1170 715 632 494 458 360
RsrII CG'GwC_CG
Cuts at: 5640 5640
Size: 7445
SacI G_AGCT'C
Cuts at: 440 1894 2114 3769 4459 440
Size: 1454 220 1655 690 3426
Fragments arranged by size:
3426 1655 1454 690 220
SalI G'TCGA_C
Cuts at: 4482 4482
Size: 7445
SanDI GG'GwC_CC
Cuts at: 3811 3811
Size: 7445
SapI GCTCTTCn'nnn_
Cuts at: 217 802 5468 5678 217
Size: 585 4666 210 1984
Fragments arranged by size:
4666 1984 585 210
SbfI CC_TGCA'GG
Cuts at: 1988 4492 1988
Size: 2504 4941
ScaI AGT'ACT
Cuts at: 6962 6962
Size: 7445
SfcI C'TryA_G
Cuts at: 33 534 1654 1748 1984 4488 5173 6166 6357
Size: 501 1120 94 236 2504 685 993 191
Cuts at: 6357 33
Size: 1121
Fragments arranged by size:
2504 1121 1120 993 685 501 236 191
94
SfoI GGC'GCC
Cuts at: 632 1851 4877 5125 632
Size: 1219 3026 248 2952
Fragments arranged by size:
3026 2952 1219 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7445
SgrAI Cr'CCGG_yG
Cuts at: 1216 1216
Size: 7445
SmaI CCC'GGG
Cuts at: 1323 4467 1323
Size: 3144 4301
SmlI C'TyrA_G
Cuts at: 856 866 970 1777 1887 2164 3007 4600 5515
Size: 10 104 807 110 277 843 1593 915
Cuts at: 5515 6007 6269 856
Size: 492 262 2032
Fragments arranged by size:
2032 1593 915 843 807 492 277 262
110 104 10
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7445
SphI G_CATG'C
Cuts at: 4498 4998 4498
Size: 500 6945
SspI AAT'ATT
Cuts at: 1028 1028
Size: 7445
StuI AGG'CCT
Cuts at: 1832 3417 1832
Size: 1585 5860
StyI C'CwwG_G
Cuts at: 542 944 1187 1787 4214 4579 4933 542
Size: 402 243 600 2427 365 354 3054
Fragments arranged by size:
3054 2427 600 402 365 354 243
SwaI ATTT'AAAT
Cuts at: 1904 1904
Size: 7445
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2360 2835 3096 3339 3751 4021 4261 5028 5161
Size: 475 261 243 412 270 240 767 133
Cuts at: 5161 5620 5829 2360
Size: 459 209 3976
Fragments arranged by size:
3976 767 475 459 412 270 261 243
240 209 133
TatI w'GTAC_w
Cuts at: 2751 3731 6960 2751
Size: 980 3229 3236
Fragments arranged by size:
3236 3229 980
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 876 908 2667 2699 2827 2859 6962 6994 876
Size: 32 1759 32 128 32 4103 32 1327
Fragments arranged by size:
4103 1759 1327 128 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5242 5242
Size: 7445
XbaI T'CTAG_A
Cuts at: 2970 4476 2970
Size: 1506 5939
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3533 4940 3533
Size: 1407 6038
XhoI C'TCGA_G
Cuts at: 2164 3007 2164
Size: 843 6602
XmaI C'CCGG_G
Cuts at: 1321 4465 1321
Size: 3144 4301
XmnI GAAnn'nnTTC
Cuts at: 1408 2378 1408
Size: 970 6475
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SnaBI SrfI
ZraI