Restriction map for
pF1KA1207
(Circular) (Six-base) MAPSORT of: pF1KA1207.seq Check: 316 from: 1 to: 9296
pF1KA1207 9296 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AatII G_ACGT'C
Cuts at: 2754 2754
Size: 9296
AccI GT'mk_AC
Cuts at: 276 3515 6334 276
Size: 3239 2819 3238
Fragments arranged by size:
3239 3238 2819
Acc65I G'GTAC_C
Cuts at: 6312 6312
Size: 9296
AclI AA'CG_TT
Cuts at: 1600 9136 1600
Size: 7536 1760
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 1372 1388 2203 2423 2581 3338 5297 5371
Size: 981 16 815 220 158 757 1959 74
Cuts at: 5371 7121 7553 7906 8568 391
Size: 1750 432 353 662 1119
Fragments arranged by size:
1959 1750 1119 981 815 757 662 432
353 220 158 74 16
AflII C'TTAA_G
Cuts at: 2631 4044 4144 2631
Size: 1413 100 7783
Fragments arranged by size:
7783 1413 100
AflIII A'CryG_T
Cuts at: 2219 6648 8448 8729 2219
Size: 4429 1800 281 2786
Fragments arranged by size:
4429 2786 1800 281
AleI CACnn'nnGTG
Cuts at: 779 2344 3466 779
Size: 1565 1122 6609
Fragments arranged by size:
6609 1565 1122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 418 418
Size: 9296
AlwNI CAG_nnn'CTG
Cuts at: 3332 5316 6601 8039 3332
Size: 1984 1285 1438 4589
Fragments arranged by size:
4589 1984 1438 1285
ApaI G_GGCC'C
Cuts at: 521 598 605 2013 5274 521
Size: 77 7 1408 3261 4543
Fragments arranged by size:
4543 3261 1408 77 7
ApaLI G'TGCA_C
Cuts at: 4281 8134 4281
Size: 3853 5443
ApoI r'AATT_y
Cuts at: 154 943 2150 2477 2797 3286 3781 4474 4576
Size: 789 1207 327 320 489 495 693 102
Cuts at: 4576 4964 5233 5634 5734 6300 8649 8905 154
Size: 388 269 401 100 566 2349 256 545
Fragments arranged by size:
2349 1207 789 693 566 545 495 489
401 388 327 320 269 256 102 100
AseI AT'TA_AT
Cuts at: 20 346 2389 20
Size: 326 2043 6927
Fragments arranged by size:
6927 2043 326
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9296
AvaI C'yCGr_G
Cuts at: 2932 4994 5266 6316 2932
Size: 2062 272 1050 5912
Fragments arranged by size:
5912 2062 1050 272
BamHI G'GATC_C
Cuts at: 168 1293 6321 168
Size: 1125 5028 3143
Fragments arranged by size:
5028 3143 1125
BanI G'GyrC_C
Cuts at: 2595 3394 3857 3966 5980 6170 6312 6726 6974
Size: 799 463 109 2014 190 142 414 248
Cuts at: 6974 7009 2595
Size: 35 4882
Fragments arranged by size:
4882 2014 799 463 414 248 190 142
109 35
BanII G_rGCy'C
Cuts at: 521 598 605 1775 2013 4675 4802 5274 6310
Size: 77 7 1170 238 2662 127 472 1036
Cuts at: 6310 7340 521
Size: 1030 2477
Fragments arranged by size:
2662 2477 1170 1036 1030 472 238 127
77 7
BbeI G_GCGC'C
Cuts at: 3970 5984 6174 6730 6978 3970
Size: 2014 190 556 248 6288
Fragments arranged by size:
6288 2014 556 248 190
BbsI GAAGACnn'nnnn_
Cuts at: 575 1753 2213 4156 4412 4877 575
Size: 1178 460 1943 256 465 4994
Fragments arranged by size:
4994 1943 1178 465 460 256
BbvCI CC'TCA_GC
Cuts at: 1208 1208
Size: 9296
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 9262
BciVI GTATCCnnnnn_n'
Cuts at: 2132 2576 7194 8250 9296 2132
Size: 444 4618 1056 1046 2132
Fragments arranged by size:
4618 2132 1056 1046 444
BclI T'GATC_A
Cuts at: 3111 4133 4229 5483 5756 6816 3111
Size: 1022 96 1254 273 1060 5591
Fragments arranged by size:
5591 1254 1060 1022 273 96
BfrBI ATG'CAT
Cuts at: 2521 3043 2521
Size: 522 8774
BglI GCCn_nnn'nGGC
Cuts at: 609 3403 609
Size: 2794 6502
BglII A'GATC_T
Cuts at: 5614 6811 8848 5614
Size: 1197 2037 6062
Fragments arranged by size:
6062 2037 1197
BlpI GC'TnA_GC
Cuts at: 635 6408 635
Size: 5773 3523
Bme1580I G_kGCm'C
Cuts at: 521 598 605 2013 3399 4285 5274 6921 7014
Size: 77 7 1408 1386 886 989 1647 93
Cuts at: 7014 8138 521
Size: 1124 1679
Fragments arranged by size:
1679 1647 1408 1386 1124 989 886 93
77 7
BmgBI CAC'GTC
Cuts at: 777 777
Size: 9296
BmrI ACTGGGnnnn_n'
Cuts at: 458 1449 1553 2055 3856 4082 4861 4900 6508
Size: 991 104 502 1801 226 779 39 1608
Cuts at: 6508 6589 6924 9068 458
Size: 81 335 2144 686
Fragments arranged by size:
2144 1801 1608 991 779 686 502 335
226 104 81 39
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7663 7663
Size: 9296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3002 3354 3460 4786 5114 5736 3002
Size: 352 106 1326 328 622 6562
Fragments arranged by size:
6562 1326 622 352 328 106
Bpu10I CC'TnA_GC
Cuts at: 511 1208 5324 511
Size: 697 4116 4483
Fragments arranged by size:
4483 4116 697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 288 1762 1789 2773 4525 4931 6472 7351 8065
Size: 1474 27 984 1752 406 1541 879 714
Cuts at: 8065 8363 288
Size: 298 1221
Fragments arranged by size:
1752 1541 1474 1221 984 879 714 406
298 27
BsaI GGTCTCn'nnnn_
Cuts at: 2297 5115 9000 2297
Size: 2818 3885 2593
Fragments arranged by size:
3885 2818 2593
BsaAI yAC'GTr
Cuts at: 3662 5331 6649 3662
Size: 1669 1318 6309
Fragments arranged by size:
6309 1669 1318
BsaBI GATnn'nnATC
Cuts at: 6 6810 8847 6
Size: 6804 2037 455
Fragments arranged by size:
6804 2037 455
BsaHI Gr'CG_yC
Cuts at: 2751 3967 5981 6171 6727 6975 7677 9173 2751
Size: 1216 2014 190 556 248 702 1496 2874
Fragments arranged by size:
2874 2014 1496 1216 702 556 248 190
BsaWI w'CCGG_w
Cuts at: 1081 1172 5880 6485 7006 7684 8095 8242 8516
Size: 91 4708 605 521 678 411 147 274
Cuts at: 8516 1081
Size: 1861
Fragments arranged by size:
4708 1861 678 605 521 411 274 147
91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2635 2665 3000 3030 3090 3120 4103 4133 4195
Size: 30 335 30 60 30 983 30 62
Cuts at: 4195 4225 2635
Size: 30 7706
Fragments arranged by size:
7706 983 335 62 60 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 554 1085 3351 4124 5092 6229 554
Size: 531 2266 773 968 1137 3621
Fragments arranged by size:
3621 2266 1137 968 773 531
BseYI C'CCAG_C
Cuts at: 520 2819 2998 3083 3257 4705 5995 6721 7479
Size: 2299 179 85 174 1448 1290 726 758
Cuts at: 7479 8144 8552 520
Size: 665 408 1264
Fragments arranged by size:
2299 1448 1290 1264 758 726 665 408
179 174 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3389 5239 3389
Size: 1850 7446
BsiEI CG_ry'CG
Cuts at: 104 2954 6838 6884 8114 104
Size: 2850 3884 46 1230 1286
Fragments arranged by size:
3884 2850 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 4285 4802 5330 6239 6310 7088 7278 8138 4285
Size: 517 528 909 71 778 190 860 5443
Fragments arranged by size:
5443 909 860 778 528 517 190 71
BsmI GAATG_Cn'
Cuts at: 2523 4768 2523
Size: 2245 7051
BsmBI CGTCTCn'nnnn_
Cuts at: 1098 1098
Size: 9296
Bsp1286I G_dGCh'C
Cuts at: 521 598 605 1775 2013 3399 4285 4675 4802
Size: 77 7 1170 238 1386 886 390 127
Cuts at: 4802 5274 5330 6239 6310 6921 7014 7088 7278
Size: 472 56 909 71 611 93 74 190
Cuts at: 7278 7340 8138 521
Size: 62 798 1679
Fragments arranged by size:
1679 1386 1170 909 886 798 611 472
390 238 190 127 93 77 74 71
62 56 7
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 9296
BspHI T'CATG_A
Cuts at: 992 4181 7728 992
Size: 3189 3547 2560
Fragments arranged by size:
3547 3189 2560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1799 2872 5483 5855 6346 6560 6862 7243 1799
Size: 1073 2611 372 491 214 302 381 3852
Fragments arranged by size:
3852 2611 1073 491 381 372 302 214
BsrBI CCG'CTC
Cuts at: 50 515 3067 5283 7588 7642 9126 9291 50
Size: 465 2552 2216 2305 54 1484 165 55
Fragments arranged by size:
2552 2305 2216 1484 465 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1163 1275 5583 7208 1163
Size: 112 4308 1625 3251
Fragments arranged by size:
4308 3251 1625 112
BsrFI r'CCGG_y
Cuts at: 671 2949 7294 7475 671
Size: 2278 4345 181 2492
Fragments arranged by size:
4345 2492 2278 181
BsrGI T'GTAC_A
Cuts at: 1333 3977 4211 1333
Size: 2644 234 6418
Fragments arranged by size:
6418 2644 234
BssSI C'ACGA_G
Cuts at: 1288 2211 7567 8275 1288
Size: 923 5356 708 2309
Fragments arranged by size:
5356 2309 923 708
BstAPI GCAn_nnn'nTGC
Cuts at: 6673 6673
Size: 9296
BstBI TT'CG_AA
Cuts at: 5739 7657 5739
Size: 1918 7378
BstEII G'GTnAC_C
Cuts at: 385 1179 385
Size: 794 8502
BstXI CCAn_nnnn'nTGG
Cuts at: 465 2819 5418 465
Size: 2354 2599 4343
Fragments arranged by size:
4343 2599 2354
BstYI r'GATC_y
Cuts at: 1 168 397 707 1293 1715 2270 3588 4644
Size: 167 229 310 586 422 555 1318 1056
Cuts at: 4644 4682 5614 6321 6788 6811 7146 7392 7698
Size: 38 932 707 467 23 335 246 306
Cuts at: 7698 7710 7796 8848 1
Size: 12 86 1052 449
Fragments arranged by size:
1318 1056 1052 932 707 586 555 467
449 422 335 310 306 246 229 167
86 38 23 12
BstZ17I GTA'TAC
Cuts at: 277 277
Size: 9296
Bsu36I CC'TnA_GG
Cuts at: 4488 5302 4488
Size: 814 8482
BtgI C'CryG_G
Cuts at: 3230 3230
Size: 9296
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2047 7246 7427 7590 2047
Size: 5199 181 163 3753
Fragments arranged by size:
5199 3753 181 163
BtsI GCAGTG_nn'
Cuts at: 4016 5061 6539 4016
Size: 1045 1478 6773
Fragments arranged by size:
6773 1478 1045
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1722 1757 1722
Size: 35 9261
DraI TTT'AAA
Cuts at: 3384 6295 3384
Size: 2911 6385
DraIII CAC_nnn'GTG
Cuts at: 3367 3367
Size: 9296
DrdI GACnn_nn'nnGTC
Cuts at: 586 5554 7002 8346 586
Size: 4968 1448 1344 1536
Fragments arranged by size:
4968 1536 1448 1344
EaeI y'GGCC_r
Cuts at: 783 981 2951 4275 5956 6230 6881 7055 7446
Size: 198 1970 1324 1681 274 651 174 391
Cuts at: 7446 7473 8818 783
Size: 27 1345 1261
Fragments arranged by size:
1970 1681 1345 1324 1261 651 391 274
198 174 27
EagI C'GGCC_G
Cuts at: 2951 6881 2951
Size: 3930 5366
EarI CTCTTCn'nnn_
Cuts at: 2399 2452 3427 4183 5273 5297 6229 7319 7529
Size: 53 975 756 1090 24 932 1090 210
Cuts at: 7529 2399
Size: 4166
Fragments arranged by size:
4166 1090 1090 975 932 756 210 53
24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 473 690 1836 3310 4275 7504 8246 8392 9106
Size: 217 1146 1474 965 3229 742 146 714
Cuts at: 9106 473
Size: 663
Fragments arranged by size:
3229 1474 1146 965 742 714 663 217
146
EcoICRI GAG'CTC
Cuts at: 4800 6308 4800
Size: 1508 7788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 1372 1388 2203 2423 2581 3002 3338 3354
Size: 981 16 815 220 158 421 336 16
Cuts at: 3354 3460 4786 5114 5297 5371 5736 7121 7553
Size: 106 1326 328 183 74 365 1385 432
Cuts at: 7553 7906 8568 391
Size: 353 662 1119
Fragments arranged by size:
1385 1326 1119 981 815 662 432 421
365 353 336 328 220 183 158 106
74 16 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2813 4322 2813
Size: 1509 7787
EcoO109I rG'GnC_Cy
Cuts at: 375 594 595 4584 5057 5270 5271 5431 6435
Size: 219 1 3989 473 213 1 160 1004
Cuts at: 6435 375
Size: 3236
Fragments arranged by size:
3989 3236 1004 473 219 213 160 1
1
EcoRI G'AATT_C
Cuts at: 2477 6300 2477
Size: 3823 5473
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 655 3415 3597 4378 4692 5302 6114 7315 655
Size: 2760 182 781 314 610 812 1201 2636
Fragments arranged by size:
2760 2636 1201 812 781 610 314 182
FspI TGC'GCA
Cuts at: 7077 7077
Size: 9296
HaeII r_GCGC'y
Cuts at: 3970 4296 5984 6174 6730 6978 8208 8979 3970
Size: 326 1688 190 556 248 1230 771 4287
Fragments arranged by size:
4287 1688 1230 771 556 326 248 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 277 309 1095 1127 1222 1254 1516 1548 1700
Size: 32 786 32 95 32 262 32 152
Cuts at: 1700 1732 2335 2367 2506 2538 2603 2635 2832
Size: 32 603 32 139 32 65 32 197
Cuts at: 2832 2864 3000 3031 3032 3063 3503 3535 4674
Size: 32 136 31 1 31 440 32 1139
Cuts at: 4674 4706 5923 5955 7138 7168 7170 7200 277
Size: 32 1217 32 1183 30 2 30 2373
Fragments arranged by size:
2373 1217 1183 1139 786 603 440 262
197 152 139 136 95 65 32 32
32 32 32 32 32 32 32 32
32 32 31 31 30 30 2 1
HincII GTy'rAC
Cuts at: 855 3008 6335 855
Size: 2153 3327 3816
Fragments arranged by size:
3816 3327 2153
HindIII A'AGCT_T
Cuts at: 1765 2859 1765
Size: 1094 8202
KasI G'GCGC_C
Cuts at: 3966 5980 6170 6726 6974 3966
Size: 2014 190 556 248 6288
Fragments arranged by size:
6288 2014 556 248 190
KpnI G_GTAC'C
Cuts at: 6316 6316
Size: 9296
MfeI C'AATT_G
Cuts at: 329 329
Size: 9296
MluI A'CGCG_T
Cuts at: 8729 8729
Size: 9296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 557 608 939 1449 1621 2312 3962 4610 4638
Size: 51 331 510 172 691 1650 648 28
Cuts at: 4638 5849 8055 8239 557
Size: 1211 2206 184 1614
Fragments arranged by size:
2206 1650 1614 1211 691 648 510 331
184 172 51 28
MscI TGG'CCA
Cuts at: 785 983 5958 7057 785
Size: 198 4975 1099 3024
Fragments arranged by size:
4975 3024 1099 198
MslI CAynn'nnrTG
Cuts at: 503 779 2344 2440 3466 4186 4761 7285 7412
Size: 276 1565 96 1026 720 575 2524 127
Cuts at: 7412 503
Size: 2387
Fragments arranged by size:
2524 2387 1565 1026 720 575 276 127
96
MspA1I CmG'CkG
Cuts at: 2166 2397 3373 5133 6407 6721 7081 7865 8110
Size: 231 976 1760 1274 314 360 784 245
Cuts at: 8110 2166
Size: 3352
Fragments arranged by size:
3352 1760 1274 976 784 360 314 245
231
NaeI GCC'GGC
Cuts at: 673 2951 7477 673
Size: 2278 4526 2492
Fragments arranged by size:
4526 2492 2278
NarI GG'CG_CC
Cuts at: 3967 5981 6171 6727 6975 3967
Size: 2014 190 556 248 6288
Fragments arranged by size:
6288 2014 556 248 190
NcoI C'CATG_G
Cuts at: 3230 3230
Size: 9296
NdeI CA'TA_TG
Cuts at: 3594 3594
Size: 9296
NgoMIV G'CCGG_C
Cuts at: 671 2949 7475 671
Size: 2278 4526 2492
Fragments arranged by size:
4526 2492 2278
NsiI A_TGCA'T
Cuts at: 2523 3045 2523
Size: 522 8774
NspI r_CATG'y
Cuts at: 1068 2424 4218 6349 6849 8452 1068
Size: 1356 1794 2131 500 1603 1912
Fragments arranged by size:
2131 1912 1794 1603 1356 500
PasI CC'CwG_GG
Cuts at: 598 3990 5376 598
Size: 3392 1386 4518
Fragments arranged by size:
4518 3392 1386
PciI A'CATG_T
Cuts at: 8448 8448
Size: 9296
PflMI CCAn_nnn'nTGG
Cuts at: 1603 3128 4912 1603
Size: 1525 1784 5987
Fragments arranged by size:
5987 1784 1525
PmeI GTTT'AAAC
Cuts at: 6295 6295
Size: 9296
PmlI CAC'GTG
Cuts at: 3662 5331 3662
Size: 1669 7627
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7742 7774 7742
Size: 32 9264
PpuMI rG'GwC_Cy
Cuts at: 375 5057 375
Size: 4682 4614
PshAI GACnn'nnGTC
Cuts at: 5825 5825
Size: 9296
PsiI TTA'TAA
Cuts at: 905 905
Size: 9296
PspOMI G'GGCC_C
Cuts at: 517 594 601 2009 5270 517
Size: 77 7 1408 3261 4543
Fragments arranged by size:
4543 3261 1408 77 7
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1577 1609 1577
Size: 32 9264
PstI C_TGCA'G
Cuts at: 3909 6343 7028 3909
Size: 2434 685 6177
Fragments arranged by size:
6177 2434 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9296
PvuII CAG'CTG
Cuts at: 2166 2397 3373 5133 6721 7081 2166
Size: 231 976 1760 1588 360 4381
Fragments arranged by size:
4381 1760 1588 976 360 231
RsrII CG'GwC_CG
Cuts at: 7491 7491
Size: 9296
SacI G_AGCT'C
Cuts at: 4802 6310 4802
Size: 1508 7788
SalI G'TCGA_C
Cuts at: 6333 6333
Size: 9296
SanDI GG'GwC_CC
Cuts at: 5057 5057
Size: 9296
SapI GCTCTTCn'nnn_
Cuts at: 2399 3427 5273 5297 6229 7319 7529 2399
Size: 1028 1846 24 932 1090 210 4166
Fragments arranged by size:
4166 1846 1090 1028 932 210 24
SbfI CC_TGCA'GG
Cuts at: 6343 6343
Size: 9296
ScaI AGT'ACT
Cuts at: 5083 8813 5083
Size: 3730 5566
SfcI C'TryA_G
Cuts at: 33 362 1704 3721 3905 5220 6339 7024 7992
Size: 329 1342 2017 184 1315 1119 685 968
Cuts at: 7992 8183 33
Size: 191 1146
Fragments arranged by size:
2017 1342 1315 1146 1119 968 685 329
191 184
SfiI GGCCn_nnn'nGGCC
Cuts at: 609 609
Size: 9296
SfoI GGC'GCC
Cuts at: 3968 5982 6172 6728 6976 3968
Size: 2014 190 556 248 6288
Fragments arranged by size:
6288 2014 556 248 190
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9296
SmaI CCC'GGG
Cuts at: 2934 4996 6318 2934
Size: 2062 1322 5912
Fragments arranged by size:
5912 2062 1322
SmlI C'TyrA_G
Cuts at: 267 1741 1768 2631 2788 4044 4144 4540 4946
Size: 1474 27 863 157 1256 100 396 406
Cuts at: 4946 6451 7366 8080 8342 267
Size: 1505 915 714 262 1221
Fragments arranged by size:
1505 1474 1256 1221 915 863 714 406
396 262 157 100 27
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9296
SphI G_CATG'C
Cuts at: 2424 6349 6849 2424
Size: 3925 500 4871
Fragments arranged by size:
4871 3925 500
SspI AAT'ATT
Cuts at: 1259 1259
Size: 9296
StuI AGG'CCT
Cuts at: 759 4002 759
Size: 3243 6053
StyI C'CwwG_G
Cuts at: 300 605 2005 3230 3399 4199 5498 6430 6784
Size: 305 1400 1225 169 800 1299 932 354
Cuts at: 6784 300
Size: 2812
Fragments arranged by size:
2812 1400 1299 1225 932 800 354 305
169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1167 3012 3747 3858 3875 4170 4889 5385 5763
Size: 1845 735 111 17 295 719 496 378
Cuts at: 5763 6879 7012 7471 7680 1167
Size: 1116 133 459 209 2783
Fragments arranged by size:
2783 1845 1116 735 719 496 459 378
295 209 133 111 17
TatI w'GTAC_w
Cuts at: 1333 1643 1826 1961 2498 3977 4211 5081 8811
Size: 310 183 135 537 1479 234 870 3730
Cuts at: 8811 1333
Size: 1818
Fragments arranged by size:
3730 1818 1479 870 537 310 234 183
135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 686 718 2821 2853 3090 3122 4103 4135 5159
Size: 32 2103 32 237 32 981 32 1024
Cuts at: 5159 5191 5823 5855 8813 8845 686
Size: 32 632 32 2958 32 1137
Fragments arranged by size:
2958 2103 1137 1024 981 632 237 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 624 3511 4339 4750 7093 624
Size: 2887 828 411 2343 2827
Fragments arranged by size:
2887 2827 2343 828 411
XbaI T'CTAG_A
Cuts at: 6327 6327
Size: 9296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 462 3026 3558 3741 4095 4252 4293 4419 4816
Size: 2564 532 183 354 157 41 126 397
Cuts at: 4816 6791 462
Size: 1975 2967
Fragments arranged by size:
2967 2564 1975 532 397 354 183 157
126 41
XmaI C'CCGG_G
Cuts at: 2932 4994 6316 2932
Size: 2062 1322 5912
Fragments arranged by size:
5912 2062 1322
XmnI GAAnn'nnTTC
Cuts at: 2801 4060 4189 2801
Size: 1259 129 7908
Fragments arranged by size:
7908 1259 129
ZraI GAC'GTC
Cuts at: 2752 2752
Size: 9296
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AgeI AhdI AloI AscI AvrII BaeI
BmtI BsiWI BssHII ClaI EcoRV FseI FspAI HpaI
NheI NotI NruI PacI PfoI PspXI SacII SexAI
SgrAI SnaBI SrfI SwaI XhoI