Restriction map for
pF1KA1216
(Circular) (Six-base) MAPSORT of: pF1KA1216.seq Check: 8885 from: 1 to: 7217
pF1KA1216 7217 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AatII G_ACGT'C
Cuts at: 3643 3643
Size: 7217
AccI GT'mk_AC
Cuts at: 3851 4255 3851
Size: 404 6813
Acc65I G'GTAC_C
Cuts at: 4233 4233
Size: 7217
AclI AA'CG_TT
Cuts at: 7057 7057
Size: 7217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 222 704 726 1640 2168 2239 3229 3824
Size: 4 482 22 914 528 71 990 595
Cuts at: 3824 5042 5474 6221 6489 218
Size: 1218 432 747 268 946
Fragments arranged by size:
1218 990 946 914 747 595 528 482
432 268 71 22 4
AfeI AGC'GCT
Cuts at: 994 994
Size: 7217
AflIII A'CryG_T
Cuts at: 4569 5673 6650 4569
Size: 1104 977 5136
Fragments arranged by size:
5136 1104 977
AgeI A'CCGG_T
Cuts at: 5605 5605
Size: 7217
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 269 301 269
Size: 32 7185
AlwNI CAG_nnn'CTG
Cuts at: 588 661 1342 1977 2146 2218 2772 4522 6089
Size: 73 681 635 169 72 554 1750 1567
Cuts at: 6089 588
Size: 1716
Fragments arranged by size:
1750 1716 1567 681 635 554 169 73
72
ApaLI G'TGCA_C
Cuts at: 5987 5987
Size: 7217
ApoI r'AATT_y
Cuts at: 332 1557 2651 2826 3094 3832 4221 6570 6826
Size: 1225 1094 175 268 738 389 2349 256
Cuts at: 6826 332
Size: 723
Fragments arranged by size:
2349 1225 1094 738 723 389 268 256
175
AseI AT'TA_AT
Cuts at: 20 2516 2701 20
Size: 2496 185 4536
Fragments arranged by size:
4536 2496 185
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7217
AvaI C'yCGr_G
Cuts at: 4237 4237
Size: 7217
BamHI G'GATC_C
Cuts at: 837 2931 3981 4242 837
Size: 2094 1050 261 3812
Fragments arranged by size:
3812 2094 1050 261
BanI G'GyrC_C
Cuts at: 811 3755 4185 4233 4647 4895 4930 811
Size: 2944 430 48 414 248 35 3098
Fragments arranged by size:
3098 2944 430 414 248 48 35
BanII G_rGCy'C
Cuts at: 568 1590 2461 3443 4231 5261 568
Size: 1022 871 982 788 1030 2524
Fragments arranged by size:
2524 1030 1022 982 871 788
BbeI G_GCGC'C
Cuts at: 4651 4899 4651
Size: 248 6969
BbsI GAAGACnn'nnnn_
Cuts at: 778 2473 2980 3832 4084 778
Size: 1695 507 852 252 3911
Fragments arranged by size:
3911 1695 852 507 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2032 2066 3315 3349 2032
Size: 34 1249 34 5900
Fragments arranged by size:
5900 1249 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 183 295 689 729 1619 3057 5115 5876 7217
Size: 112 394 40 890 1438 2058 761 1341
Cuts at: 7217 183
Size: 183
Fragments arranged by size:
2058 1438 1341 890 761 394 183 112
40
BclI T'GATC_A
Cuts at: 3560 4737 3560
Size: 1177 6040
BfrBI ATG'CAT
Cuts at: 1546 1897 1944 2645 1546
Size: 351 47 701 6118
Fragments arranged by size:
6118 701 351 47
BglII A'GATC_T
Cuts at: 513 4732 6769 513
Size: 4219 2037 961
Fragments arranged by size:
4219 2037 961
BlpI GC'TnA_GC
Cuts at: 354 4329 354
Size: 3975 3242
Bme1580I G_kGCm'C
Cuts at: 4190 4842 4935 5991 4190
Size: 652 93 1056 5416
Fragments arranged by size:
5416 1056 652 93
BmrI ACTGGGnnnn_n'
Cuts at: 1330 1353 4429 4510 4845 6989 1330
Size: 23 3076 81 335 2144 1558
Fragments arranged by size:
3076 2144 1558 335 81 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1646 1990 2069 2291 5584 1646
Size: 344 79 222 3293 3279
Fragments arranged by size:
3293 3279 344 222 79
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1772 3079 3402 4148 1027
Size: 745 1307 323 746 4096
Fragments arranged by size:
4096 1307 746 745 323
Bpu10I CC'TnA_GC
Cuts at: 2302 2302
Size: 7217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1095 1942 4393 5272 5764 6062 448
Size: 647 847 2451 879 492 298 1603
Fragments arranged by size:
2451 1603 879 847 647 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 382 1048 3152 4166 6921 382
Size: 666 2104 1014 2755 678
Fragments arranged by size:
2755 2104 1014 678 666
BsaAI yAC'GTr
Cuts at: 4570 4570
Size: 7217
BsaBI GATnn'nnATC
Cuts at: 6 887 4731 6768 6
Size: 881 3844 2037 455
Fragments arranged by size:
3844 2037 881 455
BsaHI Gr'CG_yC
Cuts at: 3640 4648 4896 5598 7094 3640
Size: 1008 248 702 1496 3763
Fragments arranged by size:
3763 1496 1008 702 248
BsaWI w'CCGG_w
Cuts at: 1937 3042 4406 4927 5605 5879 6026 6437 1937
Size: 1105 1364 521 678 274 147 411 2717
Fragments arranged by size:
2717 1364 1105 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1000 1030 1577 1607 1901 1931 2380 2410 1000
Size: 30 547 30 294 30 449 30 5807
Fragments arranged by size:
5807 547 449 294 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1075 2267 2404 2407 2456 1075
Size: 1192 137 3 49 5836
Fragments arranged by size:
5836 1192 137 49 3
BseYI C'CCAG_C
Cuts at: 3405 3631 4642 5400 5977 6473 3405
Size: 226 1011 758 577 496 4149
Fragments arranged by size:
4149 1011 758 577 496 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3118 3118
Size: 7217
BsiEI CG_ry'CG
Cuts at: 104 3223 4759 4805 6013 104
Size: 3119 1536 46 1208 1308
Fragments arranged by size:
3119 1536 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 568 1590 2050 2135 2840 3443 4231 5009 5199
Size: 1022 460 85 705 603 788 778 190
Cuts at: 5199 5991 568
Size: 792 1794
Fragments arranged by size:
1794 1022 792 788 778 705 603 460
190 85
BsmI GAATG_Cn'
Cuts at: 2329 3273 3483 3552 2329
Size: 944 210 69 5994
Fragments arranged by size:
5994 944 210 69
BsmBI CGTCTCn'nnnn_
Cuts at: 106 106
Size: 7217
Bsp1286I G_dGCh'C
Cuts at: 568 1590 2050 2135 2461 2840 3443 4190 4231
Size: 1022 460 85 326 379 603 747 41
Cuts at: 4231 4842 4935 5009 5199 5261 5991 568
Size: 611 93 74 190 62 730 1794
Fragments arranged by size:
1794 1022 747 730 611 603 460 379
326 190 93 85 74 62 41
BspEI T'CCGG_A
Cuts at: 6437 6437
Size: 7217
BspHI T'CATG_A
Cuts at: 273 2264 3099 3600 6393 273
Size: 1991 835 501 2793 1097
Fragments arranged by size:
2793 1991 1097 835 501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4267 4481 4783 5164 4267
Size: 214 302 381 6320
Fragments arranged by size:
6320 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5509 5563 7047 7212 50
Size: 5459 54 1484 165 55
Fragments arranged by size:
5459 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 365 1385 1733 5129 365
Size: 1020 348 3396 2453
Fragments arranged by size:
3396 2453 1020 348
BsrFI r'CCGG_y
Cuts at: 5215 5396 5605 5215
Size: 181 209 6827
Fragments arranged by size:
6827 209 181
BsrGI T'GTAC_A
Cuts at: 576 2940 576
Size: 2364 4853
BssSI C'ACGA_G
Cuts at: 3272 5488 5846 3272
Size: 2216 358 4643
Fragments arranged by size:
4643 2216 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4594 4594
Size: 7217
BstBI TT'CG_AA
Cuts at: 134 5578 134
Size: 5444 1773
BstXI CCAn_nnnn'nTGG
Cuts at: 951 2562 2840 3057 3862 951
Size: 1611 278 217 805 4306
Fragments arranged by size:
4306 1611 805 278 217
BstYI r'GATC_y
Cuts at: 1 513 837 888 1023 1660 1904 2931 2945
Size: 512 324 51 135 637 244 1027 14
Cuts at: 2945 3981 4242 4709 4732 5067 5313 6325 6411
Size: 1036 261 467 23 335 246 1012 86
Cuts at: 6411 6423 6769 1
Size: 12 346 449
Fragments arranged by size:
1036 1027 1012 637 512 467 449 346
335 324 261 246 244 135 86 51
23 14 12
BstZ17I GTA'TAC
Cuts at: 3852 3852
Size: 7217
Bsu36I CC'TnA_GG
Cuts at: 2354 2354
Size: 7217
BtgI C'CryG_G
Cuts at: 107 1040 1580 107
Size: 933 540 5744
Fragments arranged by size:
5744 933 540
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5167 5348 5511 5167
Size: 181 163 6873
Fragments arranged by size:
6873 181 163
BtsI GCAGTG_nn'
Cuts at: 2048 3566 3686 4139 4460 2048
Size: 1518 120 453 321 4805
Fragments arranged by size:
4805 1518 453 321 120
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1078 1113 1078
Size: 35 7182
DraI TTT'AAA
Cuts at: 465 974 3971 4082 4216 465
Size: 509 2997 111 134 3466
Fragments arranged by size:
3466 2997 509 134 111
DrdI GACnn_nn'nnGTC
Cuts at: 4923 5781 4923
Size: 858 6359
EaeI y'GGCC_r
Cuts at: 2714 4802 4976 5367 5394 6739 2714
Size: 2088 174 391 27 1345 3192
Fragments arranged by size:
3192 2088 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4802 4802
Size: 7217
EarI CTCTTCn'nnn_
Cuts at: 131 158 854 1178 1306 5240 5450 131
Size: 27 696 324 128 3934 210 1898
Fragments arranged by size:
3934 1898 696 324 210 128 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3661 5425 5735 5881 7027 3661
Size: 1764 310 146 1146 3851
Fragments arranged by size:
3851 1764 1146 310 146
EcoICRI GAG'CTC
Cuts at: 566 1588 3441 4229 566
Size: 1022 1853 788 3554
Fragments arranged by size:
3554 1853 1022 788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 222 704 726 1027 1640 1772 2168 2239
Size: 4 482 22 301 613 132 396 71
Cuts at: 2239 3079 3229 3402 3824 4148 5042 5474 6221
Size: 840 150 173 422 324 894 432 747
Cuts at: 6221 6489 218
Size: 268 946
Fragments arranged by size:
946 894 840 747 613 482 432 422
396 324 301 268 173 150 132 71
22 4
EcoNI CCTnn'n_nnAGG
Cuts at: 863 863
Size: 7217
EcoO109I rG'GnC_Cy
Cuts at: 233 2558 2913 4356 233
Size: 2325 355 1443 3094
Fragments arranged by size:
3094 2325 1443 355
EcoRI G'AATT_C
Cuts at: 3832 4221 3832
Size: 389 6828
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 353 709 2269 3263 3527 3829 5236 353
Size: 356 1560 994 264 302 1407 2334
Fragments arranged by size:
2334 1560 1407 994 356 302 264
FspI TGC'GCA
Cuts at: 4998 4998
Size: 7217
HaeII r_GCGC'y
Cuts at: 996 4651 4899 5921 6900 996
Size: 3655 248 1022 979 1313
Fragments arranged by size:
3655 1313 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2690 2722 2937 2969 5059 5089 5091 5121 2690
Size: 32 215 32 2090 30 2 30 4786
Fragments arranged by size:
4786 2090 215 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1364 3951 4256 1364
Size: 2587 305 4325
Fragments arranged by size:
4325 2587 305
KasI G'GCGC_C
Cuts at: 4647 4895 4647
Size: 248 6969
KpnI G_GTAC'C
Cuts at: 4237 4237
Size: 7217
MfeI C'AATT_G
Cuts at: 3551 3551
Size: 7217
MluI A'CGCG_T
Cuts at: 6650 6650
Size: 7217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3843 5888 6072 3843
Size: 2045 184 4988
Fragments arranged by size:
4988 2045 184
MscI TGG'CCA
Cuts at: 2716 4978 2716
Size: 2262 4955
MslI CAynn'nnrTG
Cuts at: 184 1896 2036 3140 5206 5333 184
Size: 1712 140 1104 2066 127 2068
Fragments arranged by size:
2068 2066 1712 1104 140 127
MspA1I CmG'CkG
Cuts at: 3409 4129 4328 4642 5002 6015 6260 3409
Size: 720 199 314 360 1013 245 4366
Fragments arranged by size:
4366 1013 720 360 314 245 199
NaeI GCC'GGC
Cuts at: 5398 5398
Size: 7217
NarI GG'CG_CC
Cuts at: 4648 4896 4648
Size: 248 6969
NcoI C'CATG_G
Cuts at: 107 1040 1580 107
Size: 933 540 5744
Fragments arranged by size:
5744 933 540
NdeI CA'TA_TG
Cuts at: 879 1160 879
Size: 281 6936
NgoMIV G'CCGG_C
Cuts at: 5396 5396
Size: 7217
NsiI A_TGCA'T
Cuts at: 1548 1899 1946 2647 1548
Size: 351 47 701 6118
Fragments arranged by size:
6118 701 351 47
NspI r_CATG'y
Cuts at: 183 1897 3748 3939 4270 4770 5677 183
Size: 1714 1851 191 331 500 907 1723
Fragments arranged by size:
1851 1723 1714 907 500 331 191
PciI A'CATG_T
Cuts at: 5673 5673
Size: 7217
PflMI CCAn_nnn'nTGG
Cuts at: 1020 1020
Size: 7217
PfoI T'CCnGG_A
Cuts at: 1688 1688
Size: 7217
PmeI GTTT'AAAC
Cuts at: 4216 4216
Size: 7217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6356 6388 6356
Size: 32 7185
PsiI TTA'TAA
Cuts at: 2065 2065
Size: 7217
PstI C_TGCA'G
Cuts at: 125 1484 2004 4134 4264 4949 125
Size: 1359 520 2130 130 685 2393
Fragments arranged by size:
2393 2130 1359 685 520 130
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7217
PvuII CAG'CTG
Cuts at: 3409 4129 4642 5002 3409
Size: 720 513 360 5624
Fragments arranged by size:
5624 720 513 360
RsrII CG'GwC_CG
Cuts at: 5412 5412
Size: 7217
SacI G_AGCT'C
Cuts at: 568 1590 3443 4231 568
Size: 1022 1853 788 3554
Fragments arranged by size:
3554 1853 1022 788
SalI G'TCGA_C
Cuts at: 4254 4254
Size: 7217
SapI GCTCTTCn'nnn_
Cuts at: 1306 5240 5450 1306
Size: 3934 210 3073
Fragments arranged by size:
3934 3073 210
SbfI CC_TGCA'GG
Cuts at: 4264 4264
Size: 7217
ScaI AGT'ACT
Cuts at: 3809 6734 3809
Size: 2925 4292
SfcI C'TryA_G
Cuts at: 33 121 780 1435 1480 2000 2323 2529 3818
Size: 88 659 655 45 520 323 206 1289
Cuts at: 3818 4130 4260 4945 5938 6129 33
Size: 312 130 685 993 191 1121
Fragments arranged by size:
1289 1121 993 685 659 655 520 323
312 206 191 130 88 45
SfoI GGC'GCC
Cuts at: 4649 4897 4649
Size: 248 6969
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7217
SmaI CCC'GGG
Cuts at: 4239 4239
Size: 7217
SmlI C'TyrA_G
Cuts at: 427 1110 1921 4372 5287 5779 6041 427
Size: 683 811 2451 915 492 262 1603
Fragments arranged by size:
2451 1603 915 811 683 492 262
SpeI A'CTAG_T
Cuts at: 67 3330 67
Size: 3263 3954
SphI G_CATG'C
Cuts at: 4270 4770 4270
Size: 500 6717
StuI AGG'CCT
Cuts at: 869 869
Size: 7217
StyI C'CwwG_G
Cuts at: 107 1040 1580 1781 2177 2561 3286 3449 4351
Size: 933 540 201 396 384 725 163 902
Cuts at: 4351 4705 107
Size: 354 2619
Fragments arranged by size:
2619 933 902 725 540 396 384 354
201 163
SwaI ATTT'AAAT
Cuts at: 3971 3971
Size: 7217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 402 1072 3844 3979 4800 4933 5392 5601 402
Size: 670 2772 135 821 133 459 209 2018
Fragments arranged by size:
2772 2018 821 670 459 209 135 133
TatI w'GTAC_w
Cuts at: 576 2940 3807 6732 576
Size: 2364 867 2925 1061
Fragments arranged by size:
2925 2364 1061 867
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6734 6766 6734
Size: 32 7185
Tth111I GACn'n_nGTC
Cuts at: 5014 5014
Size: 7217
XbaI T'CTAG_A
Cuts at: 4248 4248
Size: 7217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1587 1779 3196 3377 3584 4712 1587
Size: 192 1417 181 207 1128 4092
Fragments arranged by size:
4092 1417 1128 207 192 181
XmaI C'CCGG_G
Cuts at: 4237 4237
Size: 7217
XmnI GAAnn'nnTTC
Cuts at: 1300 3809 1300
Size: 2509 4708
ZraI GAC'GTC
Cuts at: 3641 3641
Size: 7217
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI ApaI AscI AvrII
BaeI BbvCI BglI BmgBI BmtI BsiWI BssHII BstEII
ClaI DraIII EcoRV FseI FspAI HindIII HpaI NheI
NotI NruI PacI PasI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI XhoI