Restriction map for
pF1KA1230
(Circular) (Six-base) MAPSORT of: pF1KA1230.seq Check: 3590 from: 1 to: 6146
pF1KA1230 6146 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 2472 3184 2472
Size: 712 5434
Acc65I G'GTAC_C
Cuts at: 3162 3162
Size: 6146
AclI AA'CG_TT
Cuts at: 3137 5986 3137
Size: 2849 3297
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 445 757 913 962 1537 1547 2258 2410
Size: 96 312 156 49 575 10 711 152
Cuts at: 2410 3056 3971 4403 4756 5418 349
Size: 646 915 432 353 662 1077
Fragments arranged by size:
1077 915 711 662 646 575 432 353
312 156 152 96 49 10
AflIII A'CryG_T
Cuts at: 128 1931 3059 3077 3498 5298 5579 128
Size: 1803 1128 18 421 1800 281 695
Fragments arranged by size:
1803 1800 1128 695 421 281 18
AgeI A'CCGG_T
Cuts at: 2978 2978
Size: 6146
AhdI GACnn_n'nnGTC
Cuts at: 2382 2382
Size: 6146
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2138 2138
Size: 6146
AlwNI CAG_nnn'CTG
Cuts at: 1104 1354 2442 2797 3451 4889 1104
Size: 250 1088 355 654 1438 2361
Fragments arranged by size:
2361 1438 1088 654 355 250
ApaI G_GGCC'C
Cuts at: 1982 1982
Size: 6146
ApaLI G'TGCA_C
Cuts at: 1727 4984 1727
Size: 3257 2889
ApoI r'AATT_y
Cuts at: 1945 2103 2841 3150 5499 5755 1945
Size: 158 738 309 2349 256 2336
Fragments arranged by size:
2349 2336 738 309 256 158
AseI AT'TA_AT
Cuts at: 20 20
Size: 6146
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6146
AvaI C'yCGr_G
Cuts at: 1997 3166 1997
Size: 1169 4977
BamHI G'GATC_C
Cuts at: 3171 3171
Size: 6146
BanI G'GyrC_C
Cuts at: 1441 3162 3576 3824 3859 1441
Size: 1721 414 248 35 3728
Fragments arranged by size:
3728 1721 414 248 35
BanII G_rGCy'C
Cuts at: 252 1578 1982 2095 2618 3160 4190 252
Size: 1326 404 113 523 542 1030 2208
Fragments arranged by size:
2208 1326 1030 542 523 404 113
BbeI G_GCGC'C
Cuts at: 3580 3828 3580
Size: 248 5898
BbsI GAAGACnn'nnnn_
Cuts at: 272 1564 2703 3134 272
Size: 1292 1139 431 3284
Fragments arranged by size:
3284 1292 1139 431
BbvCI CC'TCA_GC
Cuts at: 2735 2735
Size: 6146
BciVI GTATCCnnnnn_n'
Cuts at: 4044 5100 6146 4044
Size: 1056 1046 4044
Fragments arranged by size:
4044 1056 1046
BclI T'GATC_A
Cuts at: 3666 3666
Size: 6146
BglI GCCn_nnn'nGGC
Cuts at: 1623 1623
Size: 6146
BglII A'GATC_T
Cuts at: 1089 1672 1773 2160 3661 5698 1089
Size: 583 101 387 1501 2037 1537
Fragments arranged by size:
2037 1537 1501 583 387 101
BlpI GC'TnA_GC
Cuts at: 3258 3258
Size: 6146
Bme1580I G_kGCm'C
Cuts at: 1731 1982 3771 3864 4988 1731
Size: 251 1789 93 1124 2889
Fragments arranged by size:
2889 1789 1124 251 93
BmrI ACTGGGnnnn_n'
Cuts at: 1262 2283 2316 3101 3358 3439 3774 5918 1262
Size: 1021 33 785 257 81 335 2144 1490
Fragments arranged by size:
2144 1490 1021 785 335 257 81 33
BmtI G_CTAG'C
Cuts at: 2218 2218
Size: 6146
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4513 4513
Size: 6146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1235 1249 1829 2194 1235
Size: 14 580 365 5187
Fragments arranged by size:
5187 580 365 14
Bpu10I CC'TnA_GC
Cuts at: 494 1394 2735 494
Size: 900 1341 3905
Fragments arranged by size:
3905 1341 900
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 336 3322 4201 4915 5213 336
Size: 2986 879 714 298 1269
Fragments arranged by size:
2986 1269 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2000 5850 2000
Size: 3850 2296
BsaAI yAC'GTr
Cuts at: 3499 3499
Size: 6146
BsaBI GATnn'nnATC
Cuts at: 6 410 3660 5697 6
Size: 404 3250 2037 455
Fragments arranged by size:
3250 2037 455 404
BsaHI Gr'CG_yC
Cuts at: 3577 3825 4527 6023 3577
Size: 248 702 1496 3700
Fragments arranged by size:
3700 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2978 3335 3856 4534 4945 5092 5366 2978
Size: 357 521 678 411 147 274 3758
Fragments arranged by size:
3758 678 521 411 357 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 977 1007 1593 1623 977
Size: 30 586 30 5500
Fragments arranged by size:
5500 586 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1185 1970 1185
Size: 785 5361
BseYI C'CCAG_C
Cuts at: 3571 4329 4994 5402 3571
Size: 758 665 408 4315
Fragments arranged by size:
4315 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 238
Size: 6146
BsiEI CG_ry'CG
Cuts at: 104 3688 3734 4964 104
Size: 3584 46 1230 1286
Fragments arranged by size:
3584 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 252 539 1578 1731 1769 3160 3938 4128 4988
Size: 287 1039 153 38 1391 778 190 860
Cuts at: 4988 252
Size: 1410
Fragments arranged by size:
1410 1391 1039 860 778 287 190 153
38
BsmI GAATG_Cn'
Cuts at: 558 2187 2357 3028 558
Size: 1629 170 671 3676
Fragments arranged by size:
3676 1629 671 170
Bsp1286I G_dGCh'C
Cuts at: 252 539 1578 1731 1769 1982 2095 2618 3160
Size: 287 1039 153 38 213 113 523 542
Cuts at: 3160 3771 3864 3938 4128 4190 4988 252
Size: 611 93 74 190 62 798 1410
Fragments arranged by size:
1410 1039 798 611 542 523 287 213
190 153 113 93 74 62 38
BspHI T'CATG_A
Cuts at: 1874 4578 1874
Size: 2704 3442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 160 278 2049 3196 3410 3712 4093 160
Size: 118 1771 1147 214 302 381 2213
Fragments arranged by size:
2213 1771 1147 381 302 214 118
BsrBI CCG'CTC
Cuts at: 50 4438 4492 5976 6141 50
Size: 4388 54 1484 165 55
Fragments arranged by size:
4388 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4058 4058
Size: 6146
BsrFI r'CCGG_y
Cuts at: 2978 4144 4325 2978
Size: 1166 181 4799
Fragments arranged by size:
4799 1166 181
BssSI C'ACGA_G
Cuts at: 4417 5125 4417
Size: 708 5438
BstAPI GCAn_nnn'nTGC
Cuts at: 2578 3523 2578
Size: 945 5201
BstBI TT'CG_AA
Cuts at: 4507 4507
Size: 6146
BstXI CCAn_nnnn'nTGG
Cuts at: 1506 1506
Size: 6146
BstYI r'GATC_y
Cuts at: 1 326 618 1089 1261 1672 1773 2160 2233
Size: 325 292 471 172 411 101 387 73
Cuts at: 2233 3171 3638 3661 3996 4242 4548 4560 4646
Size: 938 467 23 335 246 306 12 86
Cuts at: 4646 5698 1
Size: 1052 449
Fragments arranged by size:
1052 938 471 467 449 411 387 335
325 306 292 246 172 101 86 73
23 12
Bsu36I CC'TnA_GG
Cuts at: 2443 2443
Size: 6146
BtgI C'CryG_G
Cuts at: 1637 1973 2561 2607 2762 1637
Size: 336 588 46 155 5021
Fragments arranged by size:
5021 588 336 155 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2487 4096 4277 4440 2487
Size: 1609 181 163 4193
Fragments arranged by size:
4193 1609 181 163
BtsI GCAGTG_nn'
Cuts at: 2604 3389 2604
Size: 785 5361
DraI TTT'AAA
Cuts at: 914 1698 2934 3084 3145 914
Size: 784 1236 150 61 3915
Fragments arranged by size:
3915 1236 784 150 61
DraIII CAC_nnn'GTG
Cuts at: 261 261
Size: 6146
DrdI GACnn_nn'nnGTC
Cuts at: 3852 5196 3852
Size: 1344 4802
EaeI y'GGCC_r
Cuts at: 812 1683 1795 2130 2482 2705 3731 3905 4296
Size: 871 112 335 352 223 1026 174 391
Cuts at: 4296 4323 5668 812
Size: 27 1345 1290
Fragments arranged by size:
1345 1290 1026 871 391 352 335 223
174 112 27
EagI C'GGCC_G
Cuts at: 3731 3731
Size: 6146
EarI CTCTTCn'nnn_
Cuts at: 527 1163 1295 4169 4379 527
Size: 636 132 2874 210 2294
Fragments arranged by size:
2874 2294 636 210 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4354 5096 5242 5956 4354
Size: 742 146 714 4544
Fragments arranged by size:
4544 742 714 146
EcoICRI GAG'CTC
Cuts at: 250 1576 3158 250
Size: 1326 1582 3238
Fragments arranged by size:
3238 1582 1326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 445 757 913 962 1235 1249 1537 1547
Size: 96 312 156 49 273 14 288 10
Cuts at: 1547 1829 2194 2258 2410 3056 3971 4403 4756
Size: 282 365 64 152 646 915 432 353
Cuts at: 4756 5418 349
Size: 662 1077
Fragments arranged by size:
1077 915 662 646 432 365 353 312
288 282 273 156 152 96 64 49
14 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2959 2959
Size: 6146
EcoO109I rG'GnC_Cy
Cuts at: 1601 1616 1978 1979 2439 3285 1601
Size: 15 362 1 460 846 4462
Fragments arranged by size:
4462 846 460 362 15 1
EcoRI G'AATT_C
Cuts at: 1945 2103 3150 1945
Size: 158 1047 4941
Fragments arranged by size:
4941 1047 158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4165 4165
Size: 6146
FspI TGC'GCA
Cuts at: 3927 3927
Size: 6146
HaeII r_GCGC'y
Cuts at: 3580 3828 5058 5829 3580
Size: 248 1230 771 3897
Fragments arranged by size:
3897 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 199 231 311 343 433 465 1593 1625 1803
Size: 32 80 32 90 32 1128 32 178
Cuts at: 1803 1821 1835 1853 1918 1950 2368 2400 2520
Size: 18 14 18 65 32 418 32 120
Cuts at: 2520 2552 3115 3147 3988 4018 4020 4050 199
Size: 32 563 32 841 30 2 30 2295
Fragments arranged by size:
2295 1128 841 563 418 178 120 90
80 65 32 32 32 32 32 32
32 32 30 30 18 18 14 2
HincII GTy'rAC
Cuts at: 476 1388 3185 476
Size: 912 1797 3437
Fragments arranged by size:
3437 1797 912
HindIII A'AGCT_T
Cuts at: 787 787
Size: 6146
HpaI GTT'AAC
Cuts at: 476 476
Size: 6146
KasI G'GCGC_C
Cuts at: 3576 3824 3576
Size: 248 5898
KpnI G_GTAC'C
Cuts at: 3166 3166
Size: 6146
MfeI C'AATT_G
Cuts at: 2067 2067
Size: 6146
MluI A'CGCG_T
Cuts at: 5579 5579
Size: 6146
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 125 596 2270 4905 5089 125
Size: 471 1674 2635 184 1182
Fragments arranged by size:
2635 1674 1182 471 184
MscI TGG'CCA
Cuts at: 814 2132 2707 3907 814
Size: 1318 575 1200 3053
Fragments arranged by size:
3053 1318 1200 575
MslI CAynn'nnrTG
Cuts at: 1636 4135 4262 1636
Size: 2499 127 3520
Fragments arranged by size:
3520 2499 127
MspA1I CmG'CkG
Cuts at: 661 867 1975 3257 3571 3931 4715 4960 661
Size: 206 1108 1282 314 360 784 245 1847
Fragments arranged by size:
1847 1282 1108 784 360 314 245 206
NaeI GCC'GGC
Cuts at: 4327 4327
Size: 6146
NarI GG'CG_CC
Cuts at: 3577 3825 3577
Size: 248 5898
NcoI C'CATG_G
Cuts at: 1637 2607 2762 1637
Size: 970 155 5021
Fragments arranged by size:
5021 970 155
NgoMIV G'CCGG_C
Cuts at: 4325 4325
Size: 6146
NheI G'CTAG_C
Cuts at: 2214 2214
Size: 6146
NspI r_CATG'y
Cuts at: 132 1935 3063 3081 3199 3699 5302 132
Size: 1803 1128 18 118 500 1603 976
Fragments arranged by size:
1803 1603 1128 976 500 118 18
PasI CC'CwG_GG
Cuts at: 1621 2229 1621
Size: 608 5538
PciI A'CATG_T
Cuts at: 128 1931 3059 3077 5298 128
Size: 1803 1128 18 2221 976
Fragments arranged by size:
2221 1803 1128 976 18
PflMI CCAn_nnn'nTGG
Cuts at: 2229 2229
Size: 6146
PmeI GTTT'AAAC
Cuts at: 3145 3145
Size: 6146
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4592 4624 4592
Size: 32 6114
PpuMI rG'GwC_Cy
Cuts at: 1601 2439 1601
Size: 838 5308
PspOMI G'GGCC_C
Cuts at: 1978 1978
Size: 6146
PstI C_TGCA'G
Cuts at: 141 575 1493 3193 3878 141
Size: 434 918 1700 685 2409
Fragments arranged by size:
2409 1700 918 685 434
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6146
PvuII CAG'CTG
Cuts at: 661 3571 3931 661
Size: 2910 360 2876
Fragments arranged by size:
2910 2876 360
RsrII CG'GwC_CG
Cuts at: 4341 4341
Size: 6146
SacI G_AGCT'C
Cuts at: 252 1578 3160 252
Size: 1326 1582 3238
Fragments arranged by size:
3238 1582 1326
SacII CC_GC'GG
Cuts at: 1976 1976
Size: 6146
SalI G'TCGA_C
Cuts at: 3183 3183
Size: 6146
SanDI GG'GwC_CC
Cuts at: 1601 1601
Size: 6146
SapI GCTCTTCn'nnn_
Cuts at: 527 1163 4169 4379 527
Size: 636 3006 210 2294
Fragments arranged by size:
3006 2294 636 210
SbfI CC_TGCA'GG
Cuts at: 3193 3193
Size: 6146
ScaI AGT'ACT
Cuts at: 5663 5663
Size: 6146
SexAI A'CCwGG_T
Cuts at: 482 2937 482
Size: 2455 3691
SfcI C'TryA_G
Cuts at: 33 137 571 662 1489 3189 3874 4842 5033
Size: 104 434 91 827 1700 685 968 191
Cuts at: 5033 33
Size: 1146
Fragments arranged by size:
1700 1146 968 827 685 434 191 104
91
SfoI GGC'GCC
Cuts at: 3578 3826 3578
Size: 248 5898
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6146
SmaI CCC'GGG
Cuts at: 3168 3168
Size: 6146
SmlI C'TyrA_G
Cuts at: 315 1997 3301 4216 4930 5192 315
Size: 1682 1304 915 714 262 1269
Fragments arranged by size:
1682 1304 1269 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6146
SphI G_CATG'C
Cuts at: 3199 3699 3199
Size: 500 5646
SspI AAT'ATT
Cuts at: 190 190
Size: 6146
StyI C'CwwG_G
Cuts at: 1146 1437 1637 2051 2607 2762 3280 3634 1146
Size: 291 200 414 556 155 518 354 3658
Fragments arranged by size:
3658 556 518 414 354 291 200 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1260 2691 3729 3862 4321 4530 1260
Size: 1431 1038 133 459 209 2876
Fragments arranged by size:
2876 1431 1038 459 209 133
TatI w'GTAC_w
Cuts at: 1020 2781 5661 1020
Size: 1761 2880 1505
Fragments arranged by size:
2880 1761 1505
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1278 1310 5663 5695 1278
Size: 32 4353 32 1729
Fragments arranged by size:
4353 1729 32 32
Tth111I GACn'n_nGTC
Cuts at: 3943 3943
Size: 6146
XbaI T'CTAG_A
Cuts at: 2163 2625 3177 2163
Size: 462 552 5132
Fragments arranged by size:
5132 552 462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 807 1153 1254 2042 3641 807
Size: 346 101 788 1599 3312
Fragments arranged by size:
3312 1599 788 346 101
XhoI C'TCGA_G
Cuts at: 1997 1997
Size: 6146
XmaI C'CCGG_G
Cuts at: 3166 3166
Size: 6146
XmnI GAAnn'nnTTC
Cuts at: 362 1091 1550 362
Size: 729 459 4958
Fragments arranged by size:
4958 729 459
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI
NdeI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspXI PsrI SfiI SgrAI SnaBI SrfI StuI
SwaI ZraI