Restriction map for
pF1KA1231
(Circular) (Six-base) MAPSORT of: pF1KA1231.seq Check: 1804 from: 1 to: 6296
pF1KA1231 6296 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1304 1708 3183 1304
Size: 404 1475 4417
Fragments arranged by size:
4417 1475 404
AccI GT'mk_AC
Cuts at: 1056 3334 1056
Size: 2278 4018
Acc65I G'GTAC_C
Cuts at: 3312 3312
Size: 6296
AclI AA'CG_TT
Cuts at: 6136 6136
Size: 6296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 832 1387 2245 4121 4553 5300 5568 383
Size: 449 555 858 1876 432 747 268 1111
Fragments arranged by size:
1876 1111 858 747 555 449 432 268
AflIII A'CryG_T
Cuts at: 1749 2945 3648 4752 5729 1749
Size: 1196 703 1104 977 2316
Fragments arranged by size:
2316 1196 1104 977 703
AgeI A'CCGG_T
Cuts at: 4684 4684
Size: 6296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3158 3289 3158
Size: 131 6165
AlwNI CAG_nnn'CTG
Cuts at: 171 741 810 1410 2901 3601 5168 171
Size: 570 69 600 1491 700 1567 1299
Fragments arranged by size:
1567 1491 1299 700 600 570 69
ApaLI G'TGCA_C
Cuts at: 1785 5066 1785
Size: 3281 3015
ApoI r'AATT_y
Cuts at: 857 1078 2618 2928 2967 3300 5649 5905 857
Size: 221 1540 310 39 333 2349 256 1248
Fragments arranged by size:
2349 1540 1248 333 310 256 221 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 6296
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6296
AvaI C'yCGr_G
Cuts at: 455 3316 455
Size: 2861 3435
BamHI G'GATC_C
Cuts at: 3321 3321
Size: 6296
BanI G'GyrC_C
Cuts at: 1323 2121 2329 3312 3726 3974 4009 1323
Size: 798 208 983 414 248 35 3610
Fragments arranged by size:
3610 983 798 414 248 208 35
BanII G_rGCy'C
Cuts at: 580 800 2249 2855 3310 4340 580
Size: 220 1449 606 455 1030 2536
Fragments arranged by size:
2536 1449 1030 606 455 220
BbeI G_GCGC'C
Cuts at: 3730 3978 3730
Size: 248 6048
BbsI GAAGACnn'nnnn_
Cuts at: 305 662 1250 1612 305
Size: 357 588 362 4989
Fragments arranged by size:
4989 588 362 357
BbvCI CC'TCA_GC
Cuts at: 1235 2666 2762 2829 1235
Size: 1431 96 67 4702
Fragments arranged by size:
4702 1431 96 67
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2523 2557 3157 3191 2523
Size: 34 600 34 5628
Fragments arranged by size:
5628 600 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4194 4955 6296 4194
Size: 761 1341 4194
Fragments arranged by size:
4194 1341 761
BclI T'GATC_A
Cuts at: 2474 3816 2474
Size: 1342 4954
BfrBI ATG'CAT
Cuts at: 1932 1932
Size: 6296
BglI GCCn_nnn'nGGC
Cuts at: 2839 2839
Size: 6296
BglII A'GATC_T
Cuts at: 1775 3811 5848 1775
Size: 2036 2037 2223
Fragments arranged by size:
2223 2037 2036
BlpI GC'TnA_GC
Cuts at: 2305 2843 2854 3408 2305
Size: 538 11 554 5193
Fragments arranged by size:
5193 554 538 11
Bme1580I G_kGCm'C
Cuts at: 1328 1789 2332 3921 4014 5070 1328
Size: 461 543 1589 93 1056 2554
Fragments arranged by size:
2554 1589 1056 543 461 93
BmgBI CAC'GTC
Cuts at: 2003 2003
Size: 6296
BmrI ACTGGGnnnn_n'
Cuts at: 336 537 1248 2951 3508 3589 3924 6068 336
Size: 201 711 1703 557 81 335 2144 564
Fragments arranged by size:
2144 1703 711 564 557 335 201 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 4663 2263
Size: 2400 3896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 751 2306 2537 2641 3038 259
Size: 492 1555 231 104 397 3517
Fragments arranged by size:
3517 1555 492 397 231 104
Bpu10I CC'TnA_GC
Cuts at: 1235 2666 2762 2829 1235
Size: 1431 96 67 4702
Fragments arranged by size:
4702 1431 96 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 3472 4351 4843 5141 592
Size: 2880 879 492 298 1747
Fragments arranged by size:
2880 1747 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6000 6000
Size: 6296
BsaAI yAC'GTr
Cuts at: 1752 2948 3649 1752
Size: 1196 701 4399
Fragments arranged by size:
4399 1196 701
BsaBI GATnn'nnATC
Cuts at: 6 3810 5847 6
Size: 3804 2037 455
Fragments arranged by size:
3804 2037 455
BsaHI Gr'CG_yC
Cuts at: 1068 2647 3727 3975 4677 6173 1068
Size: 1579 1080 248 702 1496 1191
Fragments arranged by size:
1579 1496 1191 1080 702 248
BsaWI w'CCGG_w
Cuts at: 3485 4006 4684 4958 5105 5516 3485
Size: 521 678 274 147 411 4265
Fragments arranged by size:
4265 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1814 1844 2510 2540 2997 3027 3149 3172 3179
Size: 30 666 30 457 30 122 23 7
Cuts at: 3179 3202 1814
Size: 23 4908
Fragments arranged by size:
4908 666 457 122 30 30 30 23
23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 391
Size: 6296
BseYI C'CCAG_C
Cuts at: 2318 2432 2487 2774 2834 3721 4479 5056 5552
Size: 114 55 287 60 887 758 577 496
Cuts at: 5552 2318
Size: 3062
Fragments arranged by size:
3062 887 758 577 496 287 114 60
55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1276 2812 2959 3161 1276
Size: 1536 147 202 4411
Fragments arranged by size:
4411 1536 202 147
BsiEI CG_ry'CG
Cuts at: 104 445 1063 1965 3838 3884 5092 104
Size: 341 618 902 1873 46 1208 1308
Fragments arranged by size:
1873 1308 1208 902 618 341 46
BsiHKAI G_wGCw'C
Cuts at: 452 485 1339 1789 2249 2311 3310 4088 4278
Size: 33 854 450 460 62 999 778 190
Cuts at: 4278 5070 452
Size: 792 1678
Fragments arranged by size:
1678 999 854 792 778 460 450 190
62 33
BsmI GAATG_Cn'
Cuts at: 1398 1845 2258 1398
Size: 447 413 5436
Fragments arranged by size:
5436 447 413
BsmBI CGTCTCn'nnnn_
Cuts at: 676 676
Size: 6296
Bsp1286I G_dGCh'C
Cuts at: 452 485 580 800 1328 1339 1789 2249 2311
Size: 33 95 220 528 11 450 460 62
Cuts at: 2311 2332 2855 3310 3921 4014 4088 4278 4340
Size: 21 523 455 611 93 74 190 62
Cuts at: 4340 5070 452
Size: 730 1678
Fragments arranged by size:
1678 730 611 528 523 460 455 450
220 190 95 93 74 62 62 33
21 11
BspEI T'CCGG_A
Cuts at: 5516 5516
Size: 6296
BspHI T'CATG_A
Cuts at: 861 978 5472 861
Size: 117 4494 1685
Fragments arranged by size:
4494 1685 117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1304 1708 2549 2960 3183 3346 3560 3862 4243
Size: 404 841 411 223 163 214 302 381
Cuts at: 4243 1304
Size: 3357
Fragments arranged by size:
3357 841 411 404 381 302 223 214
163
BsrBI CCG'CTC
Cuts at: 50 1976 4588 4642 6126 6291 50
Size: 1926 2612 54 1484 165 55
Fragments arranged by size:
2612 1926 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 148 2561 4208 148
Size: 2413 1647 2236
Fragments arranged by size:
2413 2236 1647
BsrFI r'CCGG_y
Cuts at: 440 444 4294 4475 4684 440
Size: 4 3850 181 209 2052
Fragments arranged by size:
3850 2052 209 181 4
BssSI C'ACGA_G
Cuts at: 4567 4925 4567
Size: 358 5938
BstAPI GCAn_nnn'nTGC
Cuts at: 835 1625 1929 2901 3673 835
Size: 790 304 972 772 3458
Fragments arranged by size:
3458 972 790 772 304
BstBI TT'CG_AA
Cuts at: 2409 4657 2409
Size: 2248 4048
BstXI CCAn_nnnn'nTGG
Cuts at: 2809 2809
Size: 6296
BstYI r'GATC_y
Cuts at: 1 315 1775 3321 3788 3811 4146 4392 5404
Size: 314 1460 1546 467 23 335 246 1012
Cuts at: 5404 5490 5502 5848 1
Size: 86 12 346 449
Fragments arranged by size:
1546 1460 1012 467 449 346 335 314
246 86 23 12
BtgI C'CryG_G
Cuts at: 554 1503 2976 554
Size: 949 1473 3874
Fragments arranged by size:
3874 1473 949
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2319 4246 4427 4590 2319
Size: 1927 181 163 4025
Fragments arranged by size:
4025 1927 181 163
BtsI GCAGTG_nn'
Cuts at: 1456 2361 3539 1456
Size: 905 1178 4213
Fragments arranged by size:
4213 1178 905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 347 382 347
Size: 35 6261
DraI TTT'AAA
Cuts at: 1440 3295 1440
Size: 1855 4441
DraIII CAC_nnn'GTG
Cuts at: 768 1793 3184 768
Size: 1025 1391 3880
Fragments arranged by size:
3880 1391 1025
DrdI GACnn_nn'nnGTC
Cuts at: 2279 2565 4002 4860 2279
Size: 286 1437 858 3715
Fragments arranged by size:
3715 1437 858 286
EaeI y'GGCC_r
Cuts at: 442 712 759 1060 1670 2702 2745 3066 3881
Size: 270 47 301 610 1032 43 321 815
Cuts at: 3881 4055 4446 4473 5818 442
Size: 174 391 27 1345 920
Fragments arranged by size:
1345 1032 920 815 610 391 321 301
270 174 47 43 27
EagI C'GGCC_G
Cuts at: 442 1060 3881 442
Size: 618 2821 2857
Fragments arranged by size:
2857 2821 618
EarI CTCTTCn'nnn_
Cuts at: 248 1307 1406 2086 2111 4319 4529 248
Size: 1059 99 680 25 2208 210 2015
Fragments arranged by size:
2208 2015 1059 680 210 99 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1075 4504 4814 4960 6106 1075
Size: 3429 310 146 1146 1265
Fragments arranged by size:
3429 1265 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2247 3308 2247
Size: 1061 5235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 383 751 832 1387 2245 2306 2537 2641
Size: 124 368 81 555 858 61 231 104
Cuts at: 2641 3038 4121 4553 5300 5568 259
Size: 397 1083 432 747 268 987
Fragments arranged by size:
1083 987 858 747 555 432 397 368
268 231 124 104 81 61
EcoO109I rG'GnC_Cy
Cuts at: 562 3435 562
Size: 2873 3423
EcoRI G'AATT_C
Cuts at: 2618 2928 2967 3300 2618
Size: 310 39 333 5614
Fragments arranged by size:
5614 333 310 39
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4315 4315
Size: 6296
FspI TGC'GCA
Cuts at: 1360 1756 4077 1360
Size: 396 2321 3579
Fragments arranged by size:
3579 2321 396
HaeII r_GCGC'y
Cuts at: 2513 3730 3978 5000 5979 2513
Size: 1217 248 1022 979 2830
Fragments arranged by size:
2830 1217 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1521 1553 1920 1952 2997 3029 3149 3181 4138
Size: 32 367 32 1045 32 120 32 957
Cuts at: 4138 4168 4170 4200 1521
Size: 30 2 30 3617
Fragments arranged by size:
3617 1045 957 367 120 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1057 2132 3335 1057
Size: 1075 1203 4018
Fragments arranged by size:
4018 1203 1075
HpaI GTT'AAC
Cuts at: 2132 2132
Size: 6296
KasI G'GCGC_C
Cuts at: 3726 3974 3726
Size: 248 6048
KpnI G_GTAC'C
Cuts at: 3316 3316
Size: 6296
MluI A'CGCG_T
Cuts at: 5729 5729
Size: 6296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 224 2295 2465 4967 5151 224
Size: 2071 170 2502 184 1369
Fragments arranged by size:
2502 2071 1369 184 170
MscI TGG'CCA
Cuts at: 761 1672 2747 3068 4057 761
Size: 911 1075 321 989 3000
Fragments arranged by size:
3000 1075 989 911 321
MslI CAynn'nnrTG
Cuts at: 1682 2632 2788 4285 4412 1682
Size: 950 156 1497 127 3566
Fragments arranged by size:
3566 1497 950 156 127
MspA1I CmG'CkG
Cuts at: 1239 2278 2322 2470 2491 2679 3407 3721 4081
Size: 1039 44 148 21 188 728 314 360
Cuts at: 4081 5094 5339 1239
Size: 1013 245 2196
Fragments arranged by size:
2196 1039 1013 728 360 314 245 188
148 44 21
NaeI GCC'GGC
Cuts at: 4477 4477
Size: 6296
NarI GG'CG_CC
Cuts at: 3727 3975 3727
Size: 248 6048
NcoI C'CATG_G
Cuts at: 1503 1503
Size: 6296
NgoMIV G'CCGG_C
Cuts at: 4475 4475
Size: 6296
NsiI A_TGCA'T
Cuts at: 1934 1934
Size: 6296
NspI r_CATG'y
Cuts at: 397 1359 1839 1923 2073 3349 3849 4756 397
Size: 962 480 84 150 1276 500 907 1937
Fragments arranged by size:
1937 1276 962 907 500 480 150 84
PciI A'CATG_T
Cuts at: 4752 4752
Size: 6296
PflMI CCAn_nnn'nTGG
Cuts at: 2963 2963
Size: 6296
PfoI T'CCnGG_A
Cuts at: 1466 2283 1466
Size: 817 5479
PmeI GTTT'AAAC
Cuts at: 3295 3295
Size: 6296
PmlI CAC'GTG
Cuts at: 1752 1752
Size: 6296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5435 5467 5435
Size: 32 6264
PsiI TTA'TAA
Cuts at: 943 2192 943
Size: 1249 5047
PstI C_TGCA'G
Cuts at: 221 1301 1391 2906 3343 4028 221
Size: 1080 90 1515 437 685 2489
Fragments arranged by size:
2489 1515 1080 685 437 90
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6296
PvuII CAG'CTG
Cuts at: 1239 2322 2470 2679 3721 4081 1239
Size: 1083 148 209 1042 360 3454
Fragments arranged by size:
3454 1083 1042 360 209 148
RsrII CG'GwC_CG
Cuts at: 1851 4491 1851
Size: 2640 3656
SacI G_AGCT'C
Cuts at: 2249 3310 2249
Size: 1061 5235
SalI G'TCGA_C
Cuts at: 1055 3333 1055
Size: 2278 4018
SapI GCTCTTCn'nnn_
Cuts at: 1406 4319 4529 1406
Size: 2913 210 3173
Fragments arranged by size:
3173 2913 210
SbfI CC_TGCA'GG
Cuts at: 3343 3343
Size: 6296
ScaI AGT'ACT
Cuts at: 5813 5813
Size: 6296
SexAI A'CCwGG_T
Cuts at: 164 740 164
Size: 576 5720
SfcI C'TryA_G
Cuts at: 33 217 1297 1387 2501 2902 3339 4024 5017
Size: 184 1080 90 1114 401 437 685 993
Cuts at: 5017 5208 33
Size: 191 1121
Fragments arranged by size:
1121 1114 1080 993 685 437 401 191
184 90
SfoI GGC'GCC
Cuts at: 3728 3976 3728
Size: 248 6048
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6296
SmaI CCC'GGG
Cuts at: 457 3318 457
Size: 2861 3435
SmlI C'TyrA_G
Cuts at: 607 3451 4366 4858 5120 607
Size: 2844 915 492 262 1783
Fragments arranged by size:
2844 1783 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6296
SphI G_CATG'C
Cuts at: 397 2073 3349 3849 397
Size: 1676 1276 500 2844
Fragments arranged by size:
2844 1676 1276 500
StuI AGG'CCT
Cuts at: 145 1462 1804 145
Size: 1317 342 4637
Fragments arranged by size:
4637 1317 342
StyI C'CwwG_G
Cuts at: 1503 1571 3430 3784 1503
Size: 68 1859 354 4015
Fragments arranged by size:
4015 1859 354 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 556 1036 1345 2114 2249 2332 2563 3879 4012
Size: 480 309 769 135 83 231 1316 133
Cuts at: 4012 4471 4680 556
Size: 459 209 2172
Fragments arranged by size:
2172 1316 769 480 459 309 231 209
135 133 83
TatI w'GTAC_w
Cuts at: 2375 2624 3230 5811 2375
Size: 249 606 2581 2860
Fragments arranged by size:
2860 2581 606 249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2510 2542 3172 3204 5813 5845 2510
Size: 32 630 32 2609 32 2961
Fragments arranged by size:
2961 2609 630 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4093 4093
Size: 6296
XbaI T'CTAG_A
Cuts at: 3327 3327
Size: 6296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 365 789 3791 365
Size: 424 3002 2870
Fragments arranged by size:
3002 2870 424
XmaI C'CCGG_G
Cuts at: 455 3316 455
Size: 2861 3435
XmnI GAAnn'nnTTC
Cuts at: 1426 1462 1720 2971 1426
Size: 36 258 1251 4751
Fragments arranged by size:
4751 1251 258 36
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PmlI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BmtI BsiWI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI EcoNI EcoRV FseI FspAI HindIII MfeI
NdeI NheI NotI NruI PacI PasI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI