Restriction map for
pF1KA1237
(Circular) (Six-base) MAPSORT of: pF1KA1237.seq Check: 4209 from: 1 to: 7559
pF1KA1237 7559 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 746 3434 746
Size: 2688 4871
AccI GT'mk_AC
Cuts at: 2521 4597 2521
Size: 2076 5483
Acc65I G'GTAC_C
Cuts at: 3572 4575 3572
Size: 1003 6556
AclI AA'CG_TT
Cuts at: 1692 3706 7399 1692
Size: 2014 3693 1852
Fragments arranged by size:
3693 2014 1852
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1541 1562 2408 2971 3305 3341 5384 5816 6169
Size: 21 846 563 334 36 2043 432 353
Cuts at: 6169 6831 1541
Size: 662 2269
Fragments arranged by size:
2269 2043 846 662 563 432 353 334
36 21
AflII C'TTAA_G
Cuts at: 2743 2743
Size: 7559
AflIII A'CryG_T
Cuts at: 4911 6711 6992 4911
Size: 1800 281 5478
Fragments arranged by size:
5478 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1154 1154
Size: 7559
AleI CACnn'nnGTG
Cuts at: 4162 4162
Size: 7559
AlwNI CAG_nnn'CTG
Cuts at: 4864 6302 4864
Size: 1438 6121
ApaI G_GGCC'C
Cuts at: 335 363 408 335
Size: 28 45 7486
Fragments arranged by size:
7486 45 28
ApaLI G'TGCA_C
Cuts at: 853 1988 6397 853
Size: 1135 4409 2015
Fragments arranged by size:
4409 2015 1135
ApoI r'AATT_y
Cuts at: 945 1294 1321 1668 4563 6912 7168 945
Size: 349 27 347 2895 2349 256 1336
Fragments arranged by size:
2895 2349 1336 349 347 256 27
AseI AT'TA_AT
Cuts at: 20 2330 20
Size: 2310 5249
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7559
AvaI C'yCGr_G
Cuts at: 193 317 1128 4579 193
Size: 124 811 3451 3173
Fragments arranged by size:
3451 3173 811 124
AvrII C'CTAG_G
Cuts at: 4200 4200
Size: 7559
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3152 3185 3152
Size: 33 7526
BamHI G'GATC_C
Cuts at: 3544 4584 3544
Size: 1040 6519
BanI G'GyrC_C
Cuts at: 243 373 1773 1786 1921 2983 3446 3572 4575
Size: 130 1400 13 135 1062 463 126 1003
Cuts at: 4575 4989 5237 5272 243
Size: 414 248 35 2530
Fragments arranged by size:
2530 1400 1062 1003 463 414 248 135
130 126 35 13
BanII G_rGCy'C
Cuts at: 335 363 408 889 1072 2161 4190 4573 5603
Size: 28 45 481 183 1089 2029 383 1030
Cuts at: 5603 335
Size: 2291
Fragments arranged by size:
2291 2029 1089 1030 481 383 183 45
28
BbeI G_GCGC'C
Cuts at: 247 377 4993 5241 247
Size: 130 4616 248 2565
Fragments arranged by size:
4616 2565 248 130
BbsI GAAGACnn'nnnn_
Cuts at: 724 1631 1712 2540 2959 3293 3385 724
Size: 907 81 828 419 334 92 4898
Fragments arranged by size:
4898 907 828 419 334 92 81
BbvCI CC'TCA_GC
Cuts at: 3884 3884
Size: 7559
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1119 1153 1232 1266 1119
Size: 34 79 34 7412
Fragments arranged by size:
7412 79 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2583 4018 5457 6513 7559 2583
Size: 1435 1439 1056 1046 2583
Fragments arranged by size:
2583 1439 1435 1056 1046
BclI T'GATC_A
Cuts at: 5079 5079
Size: 7559
BfrBI ATG'CAT
Cuts at: 1098 2533 4353 1098
Size: 1435 1820 4304
Fragments arranged by size:
4304 1820 1435
BglI GCCn_nnn'nGGC
Cuts at: 3459 4115 3459
Size: 656 6903
BglII A'GATC_T
Cuts at: 960 5074 7111 960
Size: 4114 2037 1408
Fragments arranged by size:
4114 2037 1408
BlpI GC'TnA_GC
Cuts at: 235 2267 4671 235
Size: 2032 2404 3123
Fragments arranged by size:
3123 2404 2032
Bme1580I G_kGCm'C
Cuts at: 335 363 408 857 1778 1992 3451 5184 5277
Size: 28 45 449 921 214 1459 1733 93
Cuts at: 5277 6401 335
Size: 1124 1493
Fragments arranged by size:
1733 1493 1459 1124 921 449 214 93
45 28
BmrI ACTGGGnnnn_n'
Cuts at: 1283 1563 4771 4852 5187 7331 1283
Size: 280 3208 81 335 2144 1511
Fragments arranged by size:
3208 2144 1511 335 280 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1941 2223 4204 5926 1941
Size: 282 1981 1722 3574
Fragments arranged by size:
3574 1981 1722 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 298 563 700 1048 1182 1616 1968 4366
Size: 12 265 137 348 134 434 352 2398
Cuts at: 4366 286
Size: 3479
Fragments arranged by size:
3479 2398 434 352 348 265 137 134
12
Bpu10I CC'TnA_GC
Cuts at: 365 3884 365
Size: 3519 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4735 5614 6328 6626 4735
Size: 879 714 298 5668
Fragments arranged by size:
5668 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1968 4132 7263 1968
Size: 2164 3131 2264
Fragments arranged by size:
3131 2264 2164
BsaAI yAC'GTr
Cuts at: 1684 1935 4912 1684
Size: 251 2977 4331
Fragments arranged by size:
4331 2977 251
BsaBI GATnn'nnATC
Cuts at: 6 5073 7110 6
Size: 5067 2037 455
Fragments arranged by size:
5067 2037 455
BsaHI Gr'CG_yC
Cuts at: 244 374 4990 5238 5940 7436 244
Size: 130 4616 248 702 1496 367
Fragments arranged by size:
4616 1496 702 367 248 130
BsaWI w'CCGG_w
Cuts at: 4748 5269 5947 6358 6505 6779 4748
Size: 521 678 411 147 274 5528
Fragments arranged by size:
5528 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2374 2404 2832 2862 2374
Size: 30 428 30 7071
Fragments arranged by size:
7071 428 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 992 1062 2376 2379 2382 2385 2394 2397 2400
Size: 70 1314 3 3 3 9 3 3
Cuts at: 2400 4369 992
Size: 1969 4182
Fragments arranged by size:
4182 1969 1314 70 9 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 349 2002 3225 4984 5742 6407 6815 349
Size: 1653 1223 1759 758 665 408 1093
Fragments arranged by size:
1759 1653 1223 1093 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1460 1915 3966 1460
Size: 455 2051 5053
Fragments arranged by size:
5053 2051 455
BsiEI CG_ry'CG
Cuts at: 104 402 5101 5147 6377 104
Size: 298 4699 46 1230 1286
Fragments arranged by size:
4699 1286 1230 298 46
BsiHKAI G_wGCw'C
Cuts at: 857 1072 1867 1992 2161 2758 4573 5351 5541
Size: 215 795 125 169 597 1815 778 190
Cuts at: 5541 6401 857
Size: 860 2015
Fragments arranged by size:
2015 1815 860 795 778 597 215 190
169 125
BsmI GAATG_Cn'
Cuts at: 1557 3412 4482 1557
Size: 1855 1070 4634
Fragments arranged by size:
4634 1855 1070
Bsp1286I G_dGCh'C
Cuts at: 335 363 408 857 889 1072 1778 1867 1992
Size: 28 45 449 32 183 706 89 125
Cuts at: 1992 2161 2758 3451 4190 4573 5184 5277 5351
Size: 169 597 693 739 383 611 93 74
Cuts at: 5351 5541 5603 6401 335
Size: 190 62 798 1493
Fragments arranged by size:
1493 798 739 706 693 611 597 449
383 190 183 169 125 93 89 74
62 45 32 28
BspHI T'CATG_A
Cuts at: 2708 5991 2708
Size: 3283 4276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 746 3434 4105 4609 4823 5125 5506 746
Size: 2688 671 504 214 302 381 2799
Fragments arranged by size:
2799 2688 671 504 381 302 214
BsrBI CCG'CTC
Cuts at: 50 144 259 629 3928 5851 5905 7389 7554
Size: 94 115 370 3299 1923 54 1484 165
Cuts at: 7554 50
Size: 55
Fragments arranged by size:
3299 1923 1484 370 165 115 94 55
54
BsrDI GCAATG_nn'
Cuts at: 5471 5471
Size: 7559
BsrFI r'CCGG_y
Cuts at: 183 5557 5738 183
Size: 5374 181 2004
Fragments arranged by size:
5374 2004 181
BsrGI T'GTAC_A
Cuts at: 1587 1587
Size: 7559
BssHII G'CGCG_C
Cuts at: 120 230 120
Size: 110 7449
BssSI C'ACGA_G
Cuts at: 5830 6538 5830
Size: 708 6851
BstAPI GCAn_nnn'nTGC
Cuts at: 3938 4106 4936 3938
Size: 168 830 6561
Fragments arranged by size:
6561 830 168
BstBI TT'CG_AA
Cuts at: 5920 5920
Size: 7559
BstXI CCAn_nnnn'nTGG
Cuts at: 3780 3780
Size: 7559
BstYI r'GATC_y
Cuts at: 1 960 3544 3904 3917 4584 5051 5074 5409
Size: 959 2584 360 13 667 467 23 335
Cuts at: 5409 5655 5961 5973 6059 7111 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2584 1052 959 667 467 449 360 335
306 246 86 23 13 12
Bsu36I CC'TnA_GG
Cuts at: 951 951
Size: 7559
BtgI C'CryG_G
Cuts at: 107 327 2542 3278 3429 4175 107
Size: 220 2215 736 151 746 3491
Fragments arranged by size:
3491 2215 746 736 220 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 841 4191 5509 5690 5853 841
Size: 3350 1318 181 163 2547
Fragments arranged by size:
3350 2547 1318 181 163
BtsI GCAGTG_nn'
Cuts at: 1250 1472 1828 3438 3483 3790 4802 1250
Size: 222 356 1610 45 307 1012 4007
Fragments arranged by size:
4007 1610 1012 356 307 222 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3183 3218 3183
Size: 35 7524
DraI TTT'AAA
Cuts at: 3627 4558 3627
Size: 931 6628
DraIII CAC_nnn'GTG
Cuts at: 1684 1735 1684
Size: 51 7508
DrdI GACnn_nn'nnGTC
Cuts at: 5265 6609 5265
Size: 1344 6215
EaeI y'GGCC_r
Cuts at: 133 399 445 1898 1976 5144 5318 5709 5736
Size: 266 46 1453 78 3168 174 391 27
Cuts at: 5736 7081 133
Size: 1345 611
Fragments arranged by size:
3168 1453 1345 611 391 266 174 78
46 27
EagI C'GGCC_G
Cuts at: 399 5144 399
Size: 4745 2814
EarI CTCTTCn'nnn_
Cuts at: 550 639 934 2193 2403 2418 3360 3943 4536
Size: 89 295 1259 210 15 942 583 593
Cuts at: 4536 5582 5792 550
Size: 1046 210 2317
Fragments arranged by size:
2317 1259 1046 942 593 583 295 210
210 89 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1734 3930 5767 6509 6655 7369 1734
Size: 2196 1837 742 146 714 1924
Fragments arranged by size:
2196 1924 1837 742 714 146
EcoICRI GAG'CTC
Cuts at: 1070 2159 4571 1070
Size: 1089 2412 4058
Fragments arranged by size:
4058 2412 1089
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 298 563 700 1048 1182 1541 1562 1616
Size: 12 265 137 348 134 359 21 54
Cuts at: 1616 1968 2408 2971 3305 3341 4366 5384 5816
Size: 352 440 563 334 36 1025 1018 432
Cuts at: 5816 6169 6831 286
Size: 353 662 1014
Fragments arranged by size:
1025 1018 1014 662 563 440 432 359
353 352 348 334 265 137 134 54
36 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 368 368
Size: 7559
EcoO109I rG'GnC_Cy
Cuts at: 204 359 360 391 405 1162 2428 2498 4698
Size: 155 1 31 14 757 1266 70 2200
Cuts at: 4698 204
Size: 3065
Fragments arranged by size:
3065 2200 1266 757 155 70 31 14
1
EcoRI G'AATT_C
Cuts at: 1294 1668 4563 1294
Size: 374 2895 4290
Fragments arranged by size:
4290 2895 374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1547 5578 1547
Size: 4031 3528
FspI TGC'GCA
Cuts at: 5340 5340
Size: 7559
HaeII r_GCGC'y
Cuts at: 247 285 327 377 4993 5241 6471 7242 247
Size: 38 42 50 4616 248 1230 771 564
Fragments arranged by size:
4616 1230 771 564 248 50 42 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 947 979 1003 1035 1140 1172 1420 1452 1627
Size: 32 24 32 105 32 248 32 175
Cuts at: 1627 1659 2374 2406 3484 3516 3536 3568 5401
Size: 32 715 32 1078 32 20 32 1833
Cuts at: 5401 5431 5433 5463 947
Size: 30 2 30 3043
Fragments arranged by size:
3043 1833 1078 715 248 175 105 32
32 32 32 32 32 32 32 30
30 24 20 2
HincII GTy'rAC
Cuts at: 1565 3369 3864 4598 1565
Size: 1804 495 734 4526
Fragments arranged by size:
4526 1804 734 495
HindIII A'AGCT_T
Cuts at: 1042 1662 3199 1042
Size: 620 1537 5402
Fragments arranged by size:
5402 1537 620
KasI G'GCGC_C
Cuts at: 243 373 4989 5237 243
Size: 130 4616 248 2565
Fragments arranged by size:
4616 2565 248 130
KpnI G_GTAC'C
Cuts at: 3576 4579 3576
Size: 1003 6556
MluI A'CGCG_T
Cuts at: 6992 6992
Size: 7559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2327 2953 3560 3797 6318 6502 2327
Size: 626 607 237 2521 184 3384
Fragments arranged by size:
3384 2521 626 607 237 184
MscI TGG'CCA
Cuts at: 447 5320 447
Size: 4873 2686
MslI CAynn'nnrTG
Cuts at: 1858 2332 2713 3523 4162 4326 4358 5548 5675
Size: 474 381 810 639 164 32 1190 127
Cuts at: 5675 1858
Size: 3742
Fragments arranged by size:
3742 1190 810 639 474 381 164 127
32
MspA1I CmG'CkG
Cuts at: 165 276 329 387 1897 3040 3374 4177 4670
Size: 111 53 58 1510 1143 334 803 493
Cuts at: 4670 4984 5344 6128 6373 165
Size: 314 360 784 245 1351
Fragments arranged by size:
1510 1351 1143 803 784 493 360 334
314 245 111 58 53
NaeI GCC'GGC
Cuts at: 185 5740 185
Size: 5555 2004
NarI GG'CG_CC
Cuts at: 244 374 4990 5238 244
Size: 130 4616 248 2565
Fragments arranged by size:
4616 2565 248 130
NcoI C'CATG_G
Cuts at: 107 2542 107
Size: 2435 5124
NdeI CA'TA_TG
Cuts at: 4293 4293
Size: 7559
NgoMIV G'CCGG_C
Cuts at: 183 5738 183
Size: 5555 2004
NsiI A_TGCA'T
Cuts at: 1100 2535 4355 1100
Size: 1435 1820 4304
Fragments arranged by size:
4304 1820 1435
NspI r_CATG'y
Cuts at: 1102 4067 4612 5112 6715 1102
Size: 2965 545 500 1603 1946
Fragments arranged by size:
2965 1946 1603 545 500
PasI CC'CwG_GG
Cuts at: 2654 2654
Size: 7559
PciI A'CATG_T
Cuts at: 6711 6711
Size: 7559
PflMI CCAn_nnn'nTGG
Cuts at: 1366 1797 2543 3900 1366
Size: 431 746 1357 5025
Fragments arranged by size:
5025 1357 746 431
PmeI GTTT'AAAC
Cuts at: 4558 4558
Size: 7559
PmlI CAC'GTG
Cuts at: 1684 1684
Size: 7559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6005 6037 6005
Size: 32 7527
PpuMI rG'GwC_Cy
Cuts at: 204 391 1162 204
Size: 187 771 6601
Fragments arranged by size:
6601 771 187
PshAI GACnn'nnGTC
Cuts at: 1332 1332
Size: 7559
PspOMI G'GGCC_C
Cuts at: 331 359 404 331
Size: 28 45 7486
Fragments arranged by size:
7486 45 28
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2096 2128 2096
Size: 32 7527
PstI C_TGCA'G
Cuts at: 275 1245 1512 1896 2739 3445 4606 5291 275
Size: 970 267 384 843 706 1161 685 2543
Fragments arranged by size:
2543 1161 970 843 706 685 384 267
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7559
PvuII CAG'CTG
Cuts at: 276 3040 3374 4984 5344 276
Size: 2764 334 1610 360 2491
Fragments arranged by size:
2764 2491 1610 360 334
RsrII CG'GwC_CG
Cuts at: 5754 5754
Size: 7559
SacI G_AGCT'C
Cuts at: 1072 2161 4573 1072
Size: 1089 2412 4058
Fragments arranged by size:
4058 2412 1089
SacII CC_GC'GG
Cuts at: 330 4178 330
Size: 3848 3711
SalI G'TCGA_C
Cuts at: 4596 4596
Size: 7559
SanDI GG'GwC_CC
Cuts at: 204 391 204
Size: 187 7372
SapI GCTCTTCn'nnn_
Cuts at: 550 639 5582 5792 550
Size: 89 4943 210 2317
Fragments arranged by size:
4943 2317 210 89
SbfI CC_TGCA'GG
Cuts at: 4606 4606
Size: 7559
ScaI AGT'ACT
Cuts at: 7076 7076
Size: 7559
SfcI C'TryA_G
Cuts at: 33 271 354 981 1241 1508 1892 2735 3020
Size: 238 83 627 260 267 384 843 285
Cuts at: 3020 3035 3441 3749 4602 5287 6255 6446 33
Size: 15 406 308 853 685 968 191 1146
Fragments arranged by size:
1146 968 853 843 685 627 406 384
308 285 267 260 238 191 83 15
SfoI GGC'GCC
Cuts at: 245 375 4991 5239 245
Size: 130 4616 248 2565
Fragments arranged by size:
4616 2565 248 130
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7559
SgrAI Cr'CCGG_yG
Cuts at: 183 183
Size: 7559
SmaI CCC'GGG
Cuts at: 195 319 4581 195
Size: 124 4262 3173
Fragments arranged by size:
4262 3173 124
SmlI C'TyrA_G
Cuts at: 2743 4714 5629 6343 6605 2743
Size: 1971 915 714 262 3697
Fragments arranged by size:
3697 1971 915 714 262
SpeI A'CTAG_T
Cuts at: 67 3075 67
Size: 3008 4551
SphI G_CATG'C
Cuts at: 4612 5112 4612
Size: 500 7059
StyI C'CwwG_G
Cuts at: 107 1190 1700 1796 2542 4119 4200 4693 5047
Size: 1083 510 96 746 1577 81 493 354
Cuts at: 5047 107
Size: 2619
Fragments arranged by size:
2619 1577 1083 746 510 493 354 96
81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1467 1804 2337 5142 5275 5734 5943 1467
Size: 337 533 2805 133 459 209 3083
Fragments arranged by size:
3083 2805 533 459 337 209 133
TatI w'GTAC_w
Cuts at: 1033 1587 3218 4397 7074 1033
Size: 554 1631 1179 2677 1518
Fragments arranged by size:
2677 1631 1518 1179 554
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 727 759 7076 7108 727
Size: 32 6317 32 1178
Fragments arranged by size:
6317 1178 32 32
Tth111I GACn'n_nGTC
Cuts at: 1434 5356 1434
Size: 3922 3637
XbaI T'CTAG_A
Cuts at: 4590 4590
Size: 7559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 965 5054 965
Size: 4089 3470
XmaI C'CCGG_G
Cuts at: 193 317 4579 193
Size: 124 4262 3173
Fragments arranged by size:
4262 3173 124
XmnI GAAnn'nnTTC
Cuts at: 731 731
Size: 7559
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AgeI AlfI AloI AscI BmgBI BmtI
BsiWI BsmBI BspEI BstEII BstZ17I ClaI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI PacI PfoI
PsiI PspXI SexAI SfiI SnaBI SrfI SspI StuI
SwaI XhoI ZraI