Restriction map for
pF1KA1248
(Circular) (Six-base) MAPSORT of: pF1KA1248.seq Check: 9205 from: 1 to: 4184
pF1KA1248 4184 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1222 1222
Size: 4184
Acc65I G'GTAC_C
Cuts at: 1200 1200
Size: 4184
AclI AA'CG_TT
Cuts at: 4024 4024
Size: 4184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2009 2441 3188 3456 2009
Size: 432 747 268 2737
Fragments arranged by size:
2737 747 432 268
AflIII A'CryG_T
Cuts at: 1536 2640 3617 1536
Size: 1104 977 2103
Fragments arranged by size:
2103 1104 977
AgeI A'CCGG_T
Cuts at: 1091 2572 1091
Size: 1481 2703
AlwNI CAG_nnn'CTG
Cuts at: 309 1489 3056 309
Size: 1180 1567 1437
Fragments arranged by size:
1567 1437 1180
ApaI G_GGCC'C
Cuts at: 1151 1151
Size: 4184
ApaLI G'TGCA_C
Cuts at: 2954 2954
Size: 4184
ApoI r'AATT_y
Cuts at: 471 693 1188 3537 3793 471
Size: 222 495 2349 256 862
Fragments arranged by size:
2349 862 495 256 222
AseI AT'TA_AT
Cuts at: 20 20
Size: 4184
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
AvaI C'yCGr_G
Cuts at: 1152 1204 1152
Size: 52 4132
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 697 730 697
Size: 33 4151
BamHI G'GATC_C
Cuts at: 1209 1209
Size: 4184
BanI G'GyrC_C
Cuts at: 232 1200 1614 1862 1897 232
Size: 968 414 248 35 2519
Fragments arranged by size:
2519 968 414 248 35
BanII G_rGCy'C
Cuts at: 393 686 1151 1198 2228 393
Size: 293 465 47 1030 2349
Fragments arranged by size:
2349 1030 465 293 47
BbeI G_GCGC'C
Cuts at: 1618 1866 1618
Size: 248 3936
BciVI GTATCCnnnnn_n'
Cuts at: 2082 2843 4184 2082
Size: 761 1341 2082
Fragments arranged by size:
2082 1341 761
BclI T'GATC_A
Cuts at: 1704 1704
Size: 4184
BglI GCCn_nnn'nGGC
Cuts at: 1155 1155
Size: 4184
BglII A'GATC_T
Cuts at: 1699 3736 1699
Size: 2037 2147
BlpI GC'TnA_GC
Cuts at: 1296 1296
Size: 4184
Bme1580I G_kGCm'C
Cuts at: 1151 1160 1809 1902 2958 1151
Size: 9 649 93 1056 2377
Fragments arranged by size:
2377 1056 649 93 9
BmrI ACTGGGnnnn_n'
Cuts at: 1396 1477 1812 3956 1396
Size: 81 335 2144 1624
Fragments arranged by size:
2144 1624 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 548 2551 548
Size: 2003 2181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 527
Size: 4184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1360 2239 2731 3029 907
Size: 453 879 492 298 2062
Fragments arranged by size:
2062 879 492 453 298
BsaI GGTCTCn'nnnn_
Cuts at: 782 845 1052 1176 3888 782
Size: 63 207 124 2712 1078
Fragments arranged by size:
2712 1078 207 124 63
BsaAI yAC'GTr
Cuts at: 1537 1537
Size: 4184
BsaBI GATnn'nnATC
Cuts at: 6 1698 3735 6
Size: 1692 2037 455
Fragments arranged by size:
2037 1692 455
BsaHI Gr'CG_yC
Cuts at: 166 1615 1863 2565 4061 166
Size: 1449 248 702 1496 289
Fragments arranged by size:
1496 1449 702 289 248
BsaWI w'CCGG_w
Cuts at: 645 937 1091 1373 1894 2572 2846 2993 3404
Size: 292 154 282 521 678 274 147 411
Cuts at: 3404 645
Size: 1425
Fragments arranged by size:
1425 678 521 411 292 282 274 154
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 380 410 482 512 380
Size: 30 72 30 4052
Fragments arranged by size:
4052 72 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 154
Size: 4184
BseYI C'CCAG_C
Cuts at: 252 950 1609 2367 2944 3440 252
Size: 698 659 758 577 496 996
Fragments arranged by size:
996 758 698 659 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 1091 148
Size: 943 3241
BsiEI CG_ry'CG
Cuts at: 104 1726 1772 2980 104
Size: 1622 46 1208 1308
Fragments arranged by size:
1622 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 686 1198 1976 2166 2958 686
Size: 512 778 190 792 1912
Fragments arranged by size:
1912 792 778 512 190
Bsp1286I G_dGCh'C
Cuts at: 393 686 1151 1160 1198 1809 1902 1976 2166
Size: 293 465 9 38 611 93 74 190
Cuts at: 2166 2228 2958 393
Size: 62 730 1619
Fragments arranged by size:
1619 730 611 465 293 190 93 74
62 38 9
BspEI T'CCGG_A
Cuts at: 645 937 3404 645
Size: 292 2467 1425
Fragments arranged by size:
2467 1425 292
BspHI T'CATG_A
Cuts at: 864 3360 864
Size: 2496 1688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1234 1448 1750 2131 1234
Size: 214 302 381 3287
Fragments arranged by size:
3287 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1100 2476 2530 4014 4179 50
Size: 1050 1376 54 1484 165 55
Fragments arranged by size:
1484 1376 1050 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2096 2096
Size: 4184
BsrFI r'CCGG_y
Cuts at: 1091 2182 2363 2572 1091
Size: 1091 181 209 2703
Fragments arranged by size:
2703 1091 209 181
BssSI C'ACGA_G
Cuts at: 2455 2813 2455
Size: 358 3826
BstAPI GCAn_nnn'nTGC
Cuts at: 126 1561 126
Size: 1435 2749
BstBI TT'CG_AA
Cuts at: 2545 2545
Size: 4184
BstYI r'GATC_y
Cuts at: 1 1209 1676 1699 2034 2280 3292 3378 3390
Size: 1208 467 23 335 246 1012 86 12
Cuts at: 3390 3736 1
Size: 346 449
Fragments arranged by size:
1208 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 170 823 170
Size: 653 3531
BtgI C'CryG_G
Cuts at: 107 1163 107
Size: 1056 3128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2134 2315 2478 2134
Size: 181 163 3840
Fragments arranged by size:
3840 181 163
BtsI GCAGTG_nn'
Cuts at: 881 1427 881
Size: 546 3638
DraI TTT'AAA
Cuts at: 1183 1183
Size: 4184
DraIII CAC_nnn'GTG
Cuts at: 192 276 192
Size: 84 4100
DrdI GACnn_nn'nnGTC
Cuts at: 1038 1890 2748 1038
Size: 852 858 2474
Fragments arranged by size:
2474 858 852
EaeI y'GGCC_r
Cuts at: 176 1769 1943 2334 2361 3706 176
Size: 1593 174 391 27 1345 654
Fragments arranged by size:
1593 1345 654 391 174 27
EagI C'GGCC_G
Cuts at: 1769 1769
Size: 4184
EarI CTCTTCn'nnn_
Cuts at: 377 642 674 2207 2417 377
Size: 265 32 1533 210 2144
Fragments arranged by size:
2144 1533 265 210 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2392 2702 2848 3994 127
Size: 2265 310 146 1146 317
Fragments arranged by size:
2265 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 684 1196 684
Size: 512 3672
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 2009 2441 3188 3456 527
Size: 1482 432 747 268 1255
Fragments arranged by size:
1482 1255 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1147 1148 1323 1147
Size: 1 175 4008
Fragments arranged by size:
4008 175 1
EcoRI G'AATT_C
Cuts at: 1188 1188
Size: 4184
EcoRV GAT'ATC
Cuts at: 412 816 412
Size: 404 3780
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 4184
FspI TGC'GCA
Cuts at: 1965 1965
Size: 4184
HaeII r_GCGC'y
Cuts at: 1618 1866 2888 3867 1618
Size: 248 1022 979 1935
Fragments arranged by size:
1935 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 500 532 800 832 2026 2056 2058 2088 500
Size: 32 268 32 1194 30 2 30 2596
Fragments arranged by size:
2596 1194 268 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1223 1223
Size: 4184
KasI G'GCGC_C
Cuts at: 1614 1862 1614
Size: 248 3936
KpnI G_GTAC'C
Cuts at: 1204 1204
Size: 4184
MluI A'CGCG_T
Cuts at: 3617 3617
Size: 4184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 480 577 2855 3039 480
Size: 97 2278 184 1625
Fragments arranged by size:
2278 1625 184 97
MscI TGG'CCA
Cuts at: 1945 1945
Size: 4184
MslI CAynn'nnrTG
Cuts at: 365 869 2173 2300 365
Size: 504 1304 127 2249
Fragments arranged by size:
2249 1304 504 127
MspA1I CmG'CkG
Cuts at: 954 1295 1609 1969 2982 3227 954
Size: 341 314 360 1013 245 1911
Fragments arranged by size:
1911 1013 360 341 314 245
NaeI GCC'GGC
Cuts at: 2365 2365
Size: 4184
NarI GG'CG_CC
Cuts at: 1615 1863 1615
Size: 248 3936
NcoI C'CATG_G
Cuts at: 107 1163 107
Size: 1056 3128
NgoMIV G'CCGG_C
Cuts at: 2363 2363
Size: 4184
NspI r_CATG'y
Cuts at: 330 736 1237 1737 2644 330
Size: 406 501 500 907 1870
Fragments arranged by size:
1870 907 501 500 406
PciI A'CATG_T
Cuts at: 2640 2640
Size: 4184
PflMI CCAn_nnn'nTGG
Cuts at: 276 596 276
Size: 320 3864
PmeI GTTT'AAAC
Cuts at: 1183 1183
Size: 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 4152
PspOMI G'GGCC_C
Cuts at: 1147 1147
Size: 4184
PstI C_TGCA'G
Cuts at: 122 464 1231 1916 122
Size: 342 767 685 2390
Fragments arranged by size:
2390 767 685 342
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4184
PvuII CAG'CTG
Cuts at: 954 1609 1969 954
Size: 655 360 3169
Fragments arranged by size:
3169 655 360
RsrII CG'GwC_CG
Cuts at: 2379 2379
Size: 4184
SacI G_AGCT'C
Cuts at: 686 1198 686
Size: 512 3672
SalI G'TCGA_C
Cuts at: 1221 1221
Size: 4184
SapI GCTCTTCn'nnn_
Cuts at: 674 2207 2417 674
Size: 1533 210 2441
Fragments arranged by size:
2441 1533 210
SbfI CC_TGCA'GG
Cuts at: 1231 1231
Size: 4184
ScaI AGT'ACT
Cuts at: 991 3701 991
Size: 2710 1474
SfcI C'TryA_G
Cuts at: 33 118 460 1227 1912 2905 3096 33
Size: 85 342 767 685 993 191 1121
Fragments arranged by size:
1121 993 767 685 342 191 85
SfoI GGC'GCC
Cuts at: 1616 1864 1616
Size: 248 3936
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
SmaI CCC'GGG
Cuts at: 1154 1206 1154
Size: 52 4132
SmlI C'TyrA_G
Cuts at: 922 1339 2254 2746 3008 922
Size: 417 915 492 262 2098
Fragments arranged by size:
2098 915 492 417 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4184
SphI G_CATG'C
Cuts at: 1237 1737 1237
Size: 500 3684
StuI AGG'CCT
Cuts at: 629 982 629
Size: 353 3831
StyI C'CwwG_G
Cuts at: 107 590 657 1163 1318 1672 107
Size: 483 67 506 155 354 2619
Fragments arranged by size:
2619 506 483 354 155 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 753 922 991 1767 1900 2359 2568 753
Size: 169 69 776 133 459 209 2369
Fragments arranged by size:
2369 776 459 209 169 133 69
TatI w'GTAC_w
Cuts at: 713 761 989 3699 713
Size: 48 228 2710 1198
Fragments arranged by size:
2710 1198 228 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 380 412 3701 3733 380
Size: 32 3289 32 831
Fragments arranged by size:
3289 831 32 32
Tth111I GACn'n_nGTC
Cuts at: 1981 1981
Size: 4184
XbaI T'CTAG_A
Cuts at: 1215 1215
Size: 4184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 597 1679 597
Size: 1082 3102
XmaI C'CCGG_G
Cuts at: 1152 1204 1152
Size: 52 4132
XmnI GAAnn'nnTTC
Cuts at: 338 1137 338
Size: 799 3385
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI