Restriction map for
pF1KA1250
(Circular) (Six-base) MAPSORT of: pF1KA1250.seq Check: 210 from: 1 to: 8426
pF1KA1250 8426 bp
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3433 3433
Size: 8426
AccI GT'mk_AC
Cuts at: 4315 5464 4315
Size: 1149 7277
Acc65I G'GTAC_C
Cuts at: 5442 5442
Size: 8426
AclI AA'CG_TT
Cuts at: 5322 8266 5322
Size: 2944 5482
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 2446 2666 3095 3892 3983 4074 4559 5117
Size: 392 220 429 797 91 91 485 558
Cuts at: 5117 6251 6683 7036 7698 2054
Size: 1134 432 353 662 2782
Fragments arranged by size:
2782 1134 797 662 558 485 432 429
392 353 220 91 91
AfeI AGC'GCT
Cuts at: 968 968
Size: 8426
AflII C'TTAA_G
Cuts at: 4633 4633
Size: 8426
AflIII A'CryG_T
Cuts at: 2068 5778 7578 7859 2068
Size: 3710 1800 281 2635
Fragments arranged by size:
3710 2635 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 602 602
Size: 8426
AlwNI CAG_nnn'CTG
Cuts at: 870 2469 5731 7169 870
Size: 1599 3262 1438 2127
Fragments arranged by size:
3262 2127 1599 1438
ApaI G_GGCC'C
Cuts at: 3437 3499 4309 3437
Size: 62 810 7554
Fragments arranged by size:
7554 810 62
ApaLI G'TGCA_C
Cuts at: 358 7264 358
Size: 6906 1520
ApoI r'AATT_y
Cuts at: 770 2265 2668 3186 3729 3891 4220 5199 5430
Size: 1495 403 518 543 162 329 979 231
Cuts at: 5430 7779 8035 770
Size: 2349 256 1161
Fragments arranged by size:
2349 1495 1161 979 543 518 403 329
256 231 162
AseI AT'TA_AT
Cuts at: 20 277 20
Size: 257 8169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8426
AvaI C'yCGr_G
Cuts at: 3230 3687 4309 5446 3230
Size: 457 622 1137 6210
Fragments arranged by size:
6210 1137 622 457
AvrII C'CTAG_G
Cuts at: 3373 3373
Size: 8426
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3325 3358 3325
Size: 33 8393
BamHI G'GATC_C
Cuts at: 4527 5451 4527
Size: 924 7502
BanI G'GyrC_C
Cuts at: 487 3378 3905 4129 5442 5856 6104 6139 487
Size: 2891 527 224 1313 414 248 35 2774
Fragments arranged by size:
2891 2774 1313 527 414 248 224 35
BanII G_rGCy'C
Cuts at: 3437 3499 4061 4309 4384 5363 5440 6470 3437
Size: 62 562 248 75 979 77 1030 5393
Fragments arranged by size:
5393 1030 979 562 248 77 75 62
BbeI G_GCGC'C
Cuts at: 3382 5860 6108 3382
Size: 2478 248 5700
Fragments arranged by size:
5700 2478 248
BbsI GAAGACnn'nnnn_
Cuts at: 1553 3197 3971 5057 1553
Size: 1644 774 1086 4922
Fragments arranged by size:
4922 1644 1086 774
BbvCI CC'TCA_GC
Cuts at: 1786 3500 3659 1786
Size: 1714 159 6553
Fragments arranged by size:
6553 1714 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1952 1986 1952
Size: 34 8392
BciVI GTATCCnnnnn_n'
Cuts at: 909 2717 6324 7380 8426 909
Size: 1808 3607 1056 1046 909
Fragments arranged by size:
3607 1808 1056 1046 909
BclI T'GATC_A
Cuts at: 5946 5946
Size: 8426
BglI GCCn_nnn'nGGC
Cuts at: 2371 5006 2371
Size: 2635 5791
BglII A'GATC_T
Cuts at: 4333 5941 7978 4333
Size: 1608 2037 4781
Fragments arranged by size:
4781 2037 1608
BlpI GC'TnA_GC
Cuts at: 4787 5538 4787
Size: 751 7675
Bme1580I G_kGCm'C
Cuts at: 362 1446 3437 3499 4134 4309 6051 6144 7268
Size: 1084 1991 62 635 175 1742 93 1124
Cuts at: 7268 362
Size: 1520
Fragments arranged by size:
1991 1742 1520 1124 1084 635 175 93
62
BmgBI CAC'GTC
Cuts at: 3422 3589 3422
Size: 167 8259
BmrI ACTGGGnnnn_n'
Cuts at: 3000 3211 3709 4287 5638 5719 6054 8198 3000
Size: 211 498 578 1351 81 335 2144 3228
Fragments arranged by size:
3228 2144 1351 578 498 335 211 81
BmtI G_CTAG'C
Cuts at: 3008 3008
Size: 8426
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 988 6793 988
Size: 5805 2621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4047 4757 5162 4047
Size: 710 405 7311
Fragments arranged by size:
7311 710 405
Bpu10I CC'TnA_GC
Cuts at: 1786 3500 3659 1786
Size: 1714 159 6553
Fragments arranged by size:
6553 1714 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1519 5219 5602 6481 7195 7493 1519
Size: 3700 383 879 714 298 2452
Fragments arranged by size:
3700 2452 879 714 383 298
BsaI GGTCTCn'nnnn_
Cuts at: 8130 8130
Size: 8426
BsaAI yAC'GTr
Cuts at: 3954 5779 3954
Size: 1825 6601
BsaBI GATnn'nnATC
Cuts at: 6 695 5940 7977 6
Size: 689 5245 2037 455
Fragments arranged by size:
5245 2037 689 455
BsaHI Gr'CG_yC
Cuts at: 3379 4072 5857 6105 6807 8303 3379
Size: 693 1785 248 702 1496 3502
Fragments arranged by size:
3502 1785 1496 702 693 248
BsaWI w'CCGG_w
Cuts at: 3038 5355 5615 6136 6814 7225 7372 7646 3038
Size: 2317 260 521 678 411 147 274 3818
Fragments arranged by size:
3818 2317 678 521 411 274 260 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 677 707 677
Size: 30 8396
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1816 3675 1816
Size: 1859 6567
BseYI C'CCAG_C
Cuts at: 3702 3999 4928 5146 5851 6609 7274 7682 3702
Size: 297 929 218 705 758 665 408 4446
Fragments arranged by size:
4446 929 758 705 665 408 297 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 707 883 2290 4264 4888 623
Size: 84 176 1407 1974 624 4161
Fragments arranged by size:
4161 1974 1407 624 176 84
BsiEI CG_ry'CG
Cuts at: 104 5968 6014 7244 104
Size: 5864 46 1230 1286
Fragments arranged by size:
5864 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 362 404 3415 4061 5178 5363 5389 5440 6218
Size: 42 3011 646 1117 185 26 51 778
Cuts at: 6218 6408 7268 362
Size: 190 860 1520
Fragments arranged by size:
3011 1520 1117 860 778 646 190 185
51 42 26
BsmI GAATG_Cn'
Cuts at: 5077 5414 5077
Size: 337 8089
BsmBI CGTCTCn'nnnn_
Cuts at: 2690 2690
Size: 8426
Bsp1286I G_dGCh'C
Cuts at: 362 404 1446 3415 3437 3499 4061 4134 4309
Size: 42 1042 1969 22 62 562 73 175
Cuts at: 4309 4384 5178 5363 5389 5440 6051 6144 6218
Size: 75 794 185 26 51 611 93 74
Cuts at: 6218 6408 6470 7268 362
Size: 190 62 798 1520
Fragments arranged by size:
1969 1520 1042 798 794 611 562 190
185 175 93 75 74 73 62 62
51 42 26 22
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 8426
BspHI T'CATG_A
Cuts at: 2330 6858 2330
Size: 4528 3898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3433 5476 5690 5992 6373 3433
Size: 2043 214 302 381 5486
Fragments arranged by size:
5486 2043 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3351 6718 6772 8256 8421 50
Size: 3301 3367 54 1484 165 55
Fragments arranged by size:
3367 3301 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2507 2664 4277 6338 2507
Size: 157 1613 2061 4595
Fragments arranged by size:
4595 2061 1613 157
BsrFI r'CCGG_y
Cuts at: 6424 6605 6424
Size: 181 8245
BsrGI T'GTAC_A
Cuts at: 512 512
Size: 8426
BssSI C'ACGA_G
Cuts at: 6697 7405 6697
Size: 708 7718
BstAPI GCAn_nnn'nTGC
Cuts at: 870 5803 870
Size: 4933 3493
BstBI TT'CG_AA
Cuts at: 4097 6787 4097
Size: 2690 5736
BstEII G'GTnAC_C
Cuts at: 2883 2883
Size: 8426
BstXI CCAn_nnnn'nTGG
Cuts at: 533 1491 533
Size: 958 7468
BstYI r'GATC_y
Cuts at: 1 867 3270 4333 4527 4873 5016 5349 5451
Size: 866 2403 1063 194 346 143 333 102
Cuts at: 5451 5918 5941 6276 6522 6828 6840 6926 7978
Size: 467 23 335 246 306 12 86 1052
Cuts at: 7978 1
Size: 449
Fragments arranged by size:
2403 1063 1052 866 467 449 346 335
333 306 246 194 143 102 86 23
12
BtgI C'CryG_G
Cuts at: 680 680
Size: 8426
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6376 6557 6720 6376
Size: 181 163 8082
Fragments arranged by size:
8082 181 163
BtsI GCAGTG_nn'
Cuts at: 743 1446 1694 3034 3999 4711 5669 743
Size: 703 248 1340 965 712 958 3500
Fragments arranged by size:
3500 1340 965 958 712 703 248
ClaI AT'CG_AT
Cuts at: 2426 4481 2426
Size: 2055 6371
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1841 1876 2396 2431 1841
Size: 35 520 35 7836
Fragments arranged by size:
7836 520 35 35
DraI TTT'AAA
Cuts at: 5425 5425
Size: 8426
DrdI GACnn_nn'nnGTC
Cuts at: 6132 7476 6132
Size: 1344 7082
EaeI y'GGCC_r
Cuts at: 219 677 2363 2595 3031 3837 6011 6185 6576
Size: 458 1686 232 436 806 2174 174 391
Cuts at: 6576 6603 7948 219
Size: 27 1345 697
Fragments arranged by size:
2174 1686 1345 806 697 458 436 391
232 174 27
EagI C'GGCC_G
Cuts at: 6011 6011
Size: 8426
EarI CTCTTCn'nnn_
Cuts at: 182 772 1348 1746 2138 2739 3062 3420 4094
Size: 590 576 398 392 601 323 358 674
Cuts at: 4094 4459 4618 5382 6449 6659 182
Size: 365 159 764 1067 210 1949
Fragments arranged by size:
1949 1067 764 674 601 590 576 398
392 365 358 323 210 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4933 6634 7376 7522 8236 4933
Size: 1701 742 146 714 5123
Fragments arranged by size:
5123 1701 742 714 146
EcoICRI GAG'CTC
Cuts at: 4059 5361 5438 4059
Size: 1302 77 7047
Fragments arranged by size:
7047 1302 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 2446 2666 3095 3892 3983 4047 4074 4559
Size: 392 220 429 797 91 64 27 485
Cuts at: 4559 4757 5117 5162 6251 6683 7036 7698 2054
Size: 198 360 45 1089 432 353 662 2782
Fragments arranged by size:
2782 1089 797 662 485 432 429 392
360 353 220 198 91 64 45 27
EcoO109I rG'GnC_Cy
Cuts at: 1782 3212 3367 3434 3496 4305 4306 5565 1782
Size: 1430 155 67 62 809 1 1259 4643
Fragments arranged by size:
4643 1430 1259 809 155 67 62 1
EcoRI G'AATT_C
Cuts at: 2265 3729 5430 2265
Size: 1464 1701 5261
Fragments arranged by size:
5261 1701 1464
EcoRV GAT'ATC
Cuts at: 122 1068 122
Size: 946 7480
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 196 6445 196
Size: 6249 2177
FspI TGC'GCA
Cuts at: 2611 6207 2611
Size: 3596 4830
HaeII r_GCGC'y
Cuts at: 935 970 3382 5860 6108 7338 8109 935
Size: 35 2412 2478 248 1230 771 1252
Fragments arranged by size:
2478 2412 1252 1230 771 248 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1569 1601 2832 2864 2958 2990 3305 3337 3483
Size: 32 1231 32 94 32 315 32 146
Cuts at: 3483 3515 4463 4474 4495 4506 4537 4569 4666
Size: 32 948 11 21 11 31 32 97
Cuts at: 4666 4698 4810 4842 6268 6298 6300 6330 1569
Size: 32 112 32 1426 30 2 30 3665
Fragments arranged by size:
3665 1426 1231 948 315 146 112 97
94 32 32 32 32 32 32 32
32 31 30 30 21 11 11 2
HincII GTy'rAC
Cuts at: 393 597 2154 5465 393
Size: 204 1557 3311 3354
Fragments arranged by size:
3354 3311 1557 204
HindIII A'AGCT_T
Cuts at: 1880 4228 4573 4630 1880
Size: 2348 345 57 5676
Fragments arranged by size:
5676 2348 345 57
HpaI GTT'AAC
Cuts at: 393 393
Size: 8426
KasI G'GCGC_C
Cuts at: 3378 5856 6104 3378
Size: 2478 248 5700
Fragments arranged by size:
5700 2478 248
KpnI G_GTAC'C
Cuts at: 5446 5446
Size: 8426
MluI A'CGCG_T
Cuts at: 7859 7859
Size: 8426
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1747 3160 7185 7369 1747
Size: 1413 4025 184 2804
Fragments arranged by size:
4025 2804 1413 184
MscI TGG'CCA
Cuts at: 221 679 2365 2597 3033 6187 221
Size: 458 1686 232 436 3154 2460
Fragments arranged by size:
3154 2460 1686 458 436 232
MslI CAynn'nnrTG
Cuts at: 1153 1489 3408 6415 6542 1153
Size: 336 1919 3007 127 3037
Fragments arranged by size:
3037 3007 1919 336 127
MspA1I CmG'CkG
Cuts at: 3485 4976 5537 5851 6211 6995 7240 3485
Size: 1491 561 314 360 784 245 4671
Fragments arranged by size:
4671 1491 784 561 360 314 245
NaeI GCC'GGC
Cuts at: 6607 6607
Size: 8426
NarI GG'CG_CC
Cuts at: 3379 5857 6105 3379
Size: 2478 248 5700
Fragments arranged by size:
5700 2478 248
NcoI C'CATG_G
Cuts at: 680 680
Size: 8426
NdeI CA'TA_TG
Cuts at: 888 888
Size: 8426
NgoMIV G'CCGG_C
Cuts at: 6605 6605
Size: 8426
NheI G'CTAG_C
Cuts at: 3004 3004
Size: 8426
NspI r_CATG'y
Cuts at: 358 443 1419 2072 2610 5117 5479 5979 7582
Size: 85 976 653 538 2507 362 500 1603
Cuts at: 7582 358
Size: 1202
Fragments arranged by size:
2507 1603 1202 976 653 538 500 362
85
PacI TTA_AT'TAA
Cuts at: 281 281
Size: 8426
PciI A'CATG_T
Cuts at: 2068 7578 2068
Size: 5510 2916
PflMI CCAn_nnn'nTGG
Cuts at: 1678 1678
Size: 8426
PmeI GTTT'AAAC
Cuts at: 5425 5425
Size: 8426
PmlI CAC'GTG
Cuts at: 3954 3954
Size: 8426
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2881 2913 5238 5270 6872 6904 2881
Size: 32 2325 32 1602 32 4403
Fragments arranged by size:
4403 2325 1602 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1782 3212 3367 1782
Size: 1430 155 6841
Fragments arranged by size:
6841 1430 155
PshAI GACnn'nnGTC
Cuts at: 1389 2909 1389
Size: 1520 6906
PsiI TTA'TAA
Cuts at: 1126 1331 1126
Size: 205 8221
PspOMI G'GGCC_C
Cuts at: 3433 3495 4305 3433
Size: 62 810 7554
Fragments arranged by size:
7554 810 62
PspXI vC'TCGA_Gb
Cuts at: 3687 3687
Size: 8426
PstI C_TGCA'G
Cuts at: 5473 6158 5473
Size: 685 7741
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8426
PvuII CAG'CTG
Cuts at: 5851 6211 5851
Size: 360 8066
RsrII CG'GwC_CG
Cuts at: 6621 6621
Size: 8426
SacI G_AGCT'C
Cuts at: 4061 5363 5440 4061
Size: 1302 77 7047
Fragments arranged by size:
7047 1302 77
SalI G'TCGA_C
Cuts at: 5463 5463
Size: 8426
SapI GCTCTTCn'nnn_
Cuts at: 182 3420 4094 6449 6659 182
Size: 3238 674 2355 210 1949
Fragments arranged by size:
3238 2355 1949 674 210
SbfI CC_TGCA'GG
Cuts at: 5473 5473
Size: 8426
ScaI AGT'ACT
Cuts at: 3926 7943 3926
Size: 4017 4409
SfcI C'TryA_G
Cuts at: 33 1196 1341 2201 2717 4266 5469 6154 7122
Size: 1163 145 860 516 1549 1203 685 968
Cuts at: 7122 7313 33
Size: 191 1146
Fragments arranged by size:
1549 1203 1163 1146 968 860 685 516
191 145
SfoI GGC'GCC
Cuts at: 3380 5858 6106 3380
Size: 2478 248 5700
Fragments arranged by size:
5700 2478 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8426
SmaI CCC'GGG
Cuts at: 4311 5448 4311
Size: 1137 7289
SmlI C'TyrA_G
Cuts at: 1534 3230 3687 4633 5234 5581 6496 7210 7472
Size: 1696 457 946 601 347 915 714 262
Cuts at: 7472 1534
Size: 2488
Fragments arranged by size:
2488 1696 946 915 714 601 457 347
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8426
SphI G_CATG'C
Cuts at: 5117 5479 5979 5117
Size: 362 500 7564
Fragments arranged by size:
7564 500 362
SspI AAT'ATT
Cuts at: 1335 1373 2940 3132 1335
Size: 38 1567 192 6629
Fragments arranged by size:
6629 1567 192 38
StuI AGG'CCT
Cuts at: 5250 5250
Size: 8426
StyI C'CwwG_G
Cuts at: 680 2763 3373 3550 5560 5914 680
Size: 2083 610 177 2010 354 3192
Fragments arranged by size:
3192 2083 2010 610 354 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1497 1685 3690 6009 6142 6601 6810 1497
Size: 188 2005 2319 133 459 209 3113
Fragments arranged by size:
3113 2319 2005 459 209 188 133
TatI w'GTAC_w
Cuts at: 512 3924 7941 512
Size: 3412 4017 997
Fragments arranged by size:
4017 3412 997
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 677 709 875 907 1673 1705 4407 4439 7943
Size: 32 166 32 766 32 2702 32 3504
Cuts at: 7943 7975 677
Size: 32 1128
Fragments arranged by size:
3504 2702 1128 766 166 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2455 2476 6223 2455
Size: 21 3747 4658
Fragments arranged by size:
4658 3747 21
XbaI T'CTAG_A
Cuts at: 5457 5457
Size: 8426
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1675 5921 1675
Size: 4246 4180
XhoI C'TCGA_G
Cuts at: 3230 3687 3230
Size: 457 7969
XmaI C'CCGG_G
Cuts at: 4309 5446 4309
Size: 1137 7289
XmnI GAAnn'nnTTC
Cuts at: 158 959 1525 1561 2748 3080 5414 158
Size: 801 566 36 1187 332 2334 3170
Fragments arranged by size:
3170 2334 1187 801 566 332 36
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NspI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AgeI AhdI AleI AloI AscI BfrBI BsiWI
BssHII BstZ17I Bsu36I DraIII EcoNI FseI FspAI MfeI
NotI NruI NsiI PasI PfoI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI ZraI