Restriction map for
pF1KA1254
(Circular) (Six-base) MAPSORT of: pF1KA1254.seq Check: 8669 from: 1 to: 5384
pF1KA1254 5384 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 538 2422 538
Size: 1884 3500
Acc65I G'GTAC_C
Cuts at: 2400 2400
Size: 5384
AclI AA'CG_TT
Cuts at: 5224 5224
Size: 5384
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 698 995 3209 3641 4388 4656 653
Size: 45 297 2214 432 747 268 1381
Fragments arranged by size:
2214 1381 747 432 297 268 45
AflII C'TTAA_G
Cuts at: 1168 1168
Size: 5384
AflIII A'CryG_T
Cuts at: 2096 2736 3840 4817 2096
Size: 640 1104 977 2663
Fragments arranged by size:
2663 1104 977 640
AgeI A'CCGG_T
Cuts at: 3772 3772
Size: 5384
AleI CACnn'nnGTG
Cuts at: 2047 2047
Size: 5384
AlwNI CAG_nnn'CTG
Cuts at: 559 676 2689 4256 559
Size: 117 2013 1567 1687
Fragments arranged by size:
2013 1687 1567 117
ApaLI G'TGCA_C
Cuts at: 4154 4154
Size: 5384
ApoI r'AATT_y
Cuts at: 117 189 1599 1678 1912 1929 1968 2071 2388
Size: 72 1410 79 234 17 39 103 317
Cuts at: 2388 4737 4993 117
Size: 2349 256 508
Fragments arranged by size:
2349 1410 508 317 256 234 103 79
72 39 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 5384
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5384
AvaI C'yCGr_G
Cuts at: 2404 2404
Size: 5384
BamHI G'GATC_C
Cuts at: 2409 2409
Size: 5384
BanI G'GyrC_C
Cuts at: 1833 2400 2814 3062 3097 1833
Size: 567 414 248 35 4120
Fragments arranged by size:
4120 567 414 248 35
BanII G_rGCy'C
Cuts at: 221 520 2398 3428 221
Size: 299 1878 1030 2177
Fragments arranged by size:
2177 1878 1030 299
BbeI G_GCGC'C
Cuts at: 2818 3066 2818
Size: 248 5136
BbsI GAAGACnn'nnnn_
Cuts at: 389 509 661 686 389
Size: 120 152 25 5087
Fragments arranged by size:
5087 152 120 25
BciVI GTATCCnnnnn_n'
Cuts at: 1053 2106 3282 4043 5384 1053
Size: 1053 1176 761 1341 1053
Fragments arranged by size:
1341 1176 1053 1053 761
BclI T'GATC_A
Cuts at: 2139 2184 2904 2139
Size: 45 720 4619
Fragments arranged by size:
4619 720 45
BglI GCCn_nnn'nGGC
Cuts at: 296 296
Size: 5384
BglII A'GATC_T
Cuts at: 2899 4936 2899
Size: 2037 3347
BlpI GC'TnA_GC
Cuts at: 2496 2496
Size: 5384
Bme1580I G_kGCm'C
Cuts at: 3009 3102 4158 3009
Size: 93 1056 4235
Fragments arranged by size:
4235 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 614 891 2596 2677 3012 5156 614
Size: 277 1705 81 335 2144 842
Fragments arranged by size:
2144 1705 842 335 277 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3751 3751
Size: 5384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1069 1285 1291 1069
Size: 216 6 5162
Fragments arranged by size:
5162 216 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 2560 3439 3931 4229 454
Size: 2106 879 492 298 1609
Fragments arranged by size:
2106 1609 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 754 5088 754
Size: 4334 1050
BsaAI yAC'GTr
Cuts at: 2737 2737
Size: 5384
BsaBI GATnn'nnATC
Cuts at: 6 784 2898 4935 6
Size: 778 2114 2037 455
Fragments arranged by size:
2114 2037 778 455
BsaHI Gr'CG_yC
Cuts at: 639 2815 3063 3765 5261 639
Size: 2176 248 702 1496 762
Fragments arranged by size:
2176 1496 762 702 248
BsaWI w'CCGG_w
Cuts at: 2573 3094 3772 4046 4193 4604 2573
Size: 521 678 274 147 411 3353
Fragments arranged by size:
3353 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1216 1246 1216
Size: 30 5354
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 265
Size: 5384
BseYI C'CCAG_C
Cuts at: 2809 3567 4144 4640 2809
Size: 758 577 496 3553
Fragments arranged by size:
3553 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2926 2972 4180 104
Size: 2822 46 1208 1308
Fragments arranged by size:
2822 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2016 2398 3176 3366 4158 2016
Size: 382 778 190 792 3242
Fragments arranged by size:
3242 792 778 382 190
Bsp1286I G_dGCh'C
Cuts at: 221 520 2016 2398 3009 3102 3176 3366 3428
Size: 299 1496 382 611 93 74 190 62
Cuts at: 3428 4158 221
Size: 730 1447
Fragments arranged by size:
1496 1447 730 611 382 299 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4604 4604
Size: 5384
BspHI T'CATG_A
Cuts at: 162 4560 162
Size: 4398 986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2434 2648 2950 3331 2434
Size: 214 302 381 4487
Fragments arranged by size:
4487 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3676 3730 5214 5379 50
Size: 3626 54 1484 165 55
Fragments arranged by size:
3626 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 262 3296 262
Size: 3034 2350
BsrFI r'CCGG_y
Cuts at: 683 3382 3563 3772 683
Size: 2699 181 209 2295
Fragments arranged by size:
2699 2295 209 181
BssSI C'ACGA_G
Cuts at: 3655 4013 3655
Size: 358 5026
BstAPI GCAn_nnn'nTGC
Cuts at: 2761 2761
Size: 5384
BstBI TT'CG_AA
Cuts at: 1427 3745 1427
Size: 2318 3066
BstXI CCAn_nnnn'nTGG
Cuts at: 811 811
Size: 5384
BstYI r'GATC_y
Cuts at: 1 2409 2876 2899 3234 3480 4492 4578 4590
Size: 2408 467 23 335 246 1012 86 12
Cuts at: 4590 4936 1
Size: 346 449
Fragments arranged by size:
2408 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 539 539
Size: 5384
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3334 3515 3678 3334
Size: 181 163 5040
Fragments arranged by size:
5040 181 163
BtsI GCAGTG_nn'
Cuts at: 2627 2627
Size: 5384
DraI TTT'AAA
Cuts at: 1716 1908 2383 1716
Size: 192 475 4717
Fragments arranged by size:
4717 475 192
DrdI GACnn_nn'nnGTC
Cuts at: 3090 3948 3090
Size: 858 4526
EaeI y'GGCC_r
Cuts at: 801 2969 3143 3534 3561 4906 801
Size: 2168 174 391 27 1345 1279
Fragments arranged by size:
2168 1345 1279 391 174 27
EagI C'GGCC_G
Cuts at: 2969 2969
Size: 5384
EarI CTCTTCn'nnn_
Cuts at: 525 1079 1178 2200 3407 3617 525
Size: 554 99 1022 1207 210 2292
Fragments arranged by size:
2292 1207 1022 554 210 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3592 3902 4048 5194 3592
Size: 310 146 1146 3782
Fragments arranged by size:
3782 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2396 2396
Size: 5384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 698 995 1069 1285 1291 3209 3641 4388
Size: 45 297 74 216 6 1918 432 747
Cuts at: 4388 4656 653
Size: 268 1381
Fragments arranged by size:
1918 1381 747 432 297 268 216 74
45 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1051 1051
Size: 5384
EcoO109I rG'GnC_Cy
Cuts at: 1861 2523 1861
Size: 662 4722
EcoRI G'AATT_C
Cuts at: 189 1599 1912 2388 189
Size: 1410 313 476 3185
Fragments arranged by size:
3185 1410 476 313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 271 967 1008 1440 1752 2245 3403 271
Size: 696 41 432 312 493 1158 2252
Fragments arranged by size:
2252 1158 696 493 432 312 41
FspI TGC'GCA
Cuts at: 3165 3165
Size: 5384
HaeII r_GCGC'y
Cuts at: 2818 3066 4088 5067 2818
Size: 248 1022 979 3135
Fragments arranged by size:
3135 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 574 606 717 749 919 951 973 1005 3226
Size: 32 111 32 170 32 22 32 2221
Cuts at: 3226 3256 3258 3288 574
Size: 30 2 30 2670
Fragments arranged by size:
2670 2221 170 111 32 32 32 32
30 30 22 2
HincII GTy'rAC
Cuts at: 838 904 1235 2423 838
Size: 66 331 1188 3799
Fragments arranged by size:
3799 1188 331 66
KasI G'GCGC_C
Cuts at: 2814 3062 2814
Size: 248 5136
KpnI G_GTAC'C
Cuts at: 2404 2404
Size: 5384
MfeI C'AATT_G
Cuts at: 353 353
Size: 5384
MluI A'CGCG_T
Cuts at: 4817 4817
Size: 5384
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 127 943 2261 4055 4239 127
Size: 816 1318 1794 184 1272
Fragments arranged by size:
1794 1318 1272 816 184
MscI TGG'CCA
Cuts at: 803 3145 803
Size: 2342 3042
MslI CAynn'nnrTG
Cuts at: 809 863 2047 3373 3500 809
Size: 54 1184 1326 127 2693
Fragments arranged by size:
2693 1326 1184 127 54
MspA1I CmG'CkG
Cuts at: 212 1783 2495 2809 3169 4182 4427 212
Size: 1571 712 314 360 1013 245 1169
Fragments arranged by size:
1571 1169 1013 712 360 314 245
NaeI GCC'GGC
Cuts at: 3565 3565
Size: 5384
NarI GG'CG_CC
Cuts at: 2815 3063 2815
Size: 248 5136
NdeI CA'TA_TG
Cuts at: 2331 2331
Size: 5384
NgoMIV G'CCGG_C
Cuts at: 3563 3563
Size: 5384
NspI r_CATG'y
Cuts at: 2100 2437 2937 3844 2100
Size: 337 500 907 3640
Fragments arranged by size:
3640 907 500 337
PciI A'CATG_T
Cuts at: 2096 3840 2096
Size: 1744 3640
PmeI GTTT'AAAC
Cuts at: 2383 2383
Size: 5384
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4523 4555 4523
Size: 32 5352
PshAI GACnn'nnGTC
Cuts at: 2340 2340
Size: 5384
PsiI TTA'TAA
Cuts at: 932 932
Size: 5384
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 359 391 359
Size: 32 5352
PstI C_TGCA'G
Cuts at: 2431 3116 2431
Size: 685 4699
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5384
PvuII CAG'CTG
Cuts at: 212 1783 2809 3169 212
Size: 1571 1026 360 2427
Fragments arranged by size:
2427 1571 1026 360
RsrII CG'GwC_CG
Cuts at: 3579 3579
Size: 5384
SacI G_AGCT'C
Cuts at: 2398 2398
Size: 5384
SalI G'TCGA_C
Cuts at: 2421 2421
Size: 5384
SapI GCTCTTCn'nnn_
Cuts at: 525 2200 3407 3617 525
Size: 1675 1207 210 2292
Fragments arranged by size:
2292 1675 1207 210
SbfI CC_TGCA'GG
Cuts at: 2431 2431
Size: 5384
ScaI AGT'ACT
Cuts at: 1140 4901 1140
Size: 3761 1623
SfcI C'TryA_G
Cuts at: 33 1019 1838 2427 3112 4105 4296 33
Size: 986 819 589 685 993 191 1121
Fragments arranged by size:
1121 993 986 819 685 589 191
SfoI GGC'GCC
Cuts at: 2816 3064 2816
Size: 248 5136
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5384
SmaI CCC'GGG
Cuts at: 2406 2406
Size: 5384
SmlI C'TyrA_G
Cuts at: 433 1168 2539 3454 3946 4208 433
Size: 735 1371 915 492 262 1609
Fragments arranged by size:
1609 1371 915 735 492 262
SpeI A'CTAG_T
Cuts at: 67 1278 67
Size: 1211 4173
SphI G_CATG'C
Cuts at: 2437 2937 2437
Size: 500 4884
SspI AAT'ATT
Cuts at: 1118 1118
Size: 5384
StyI C'CwwG_G
Cuts at: 1607 2518 2872 1607
Size: 911 354 4119
Fragments arranged by size:
4119 911 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2967 3100 3559 3768 2967
Size: 133 459 209 4583
Fragments arranged by size:
4583 459 209 133
TatI w'GTAC_w
Cuts at: 1138 4899 1138
Size: 3761 1623
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2305 2337 4901 4933 2305
Size: 32 2564 32 2756
Fragments arranged by size:
2756 2564 32 32
Tth111I GACn'n_nGTC
Cuts at: 3181 3181
Size: 5384
XbaI T'CTAG_A
Cuts at: 2415 2415
Size: 5384
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1315 2879 1315
Size: 1564 3820
XmaI C'CCGG_G
Cuts at: 2404 2404
Size: 5384
XmnI GAAnn'nnTTC
Cuts at: 1678 2194 1678
Size: 516 4868
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII Bsu36I
BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI NcoI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI