Restriction map for
pF1KA1255
(Circular) (Six-base) MAPSORT of: pF1KA1255.seq Check: 2294 from: 1 to: 6746
pF1KA1255 6746 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2820 2820
Size: 6746
AatII G_ACGT'C
Cuts at: 3627 3627
Size: 6746
AccI GT'mk_AC
Cuts at: 3784 3784
Size: 6746
Acc65I G'GTAC_C
Cuts at: 3762 3762
Size: 6746
AclI AA'CG_TT
Cuts at: 6586 6586
Size: 6746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 1962 2392 4571 5003 5356 6018 463
Size: 1499 430 2179 432 353 662 1191
Fragments arranged by size:
2179 1499 1191 662 432 430 353
AfeI AGC'GCT
Cuts at: 331 852 331
Size: 521 6225
AflIII A'CryG_T
Cuts at: 1347 2273 4098 5898 6179 1347
Size: 926 1825 1800 281 1914
Fragments arranged by size:
1914 1825 1800 926 281
AhdI GACnn_n'nnGTC
Cuts at: 2088 2088
Size: 6746
AleI CACnn'nnGTG
Cuts at: 470 470
Size: 6746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 488 1319 3388 3391 488
Size: 831 2069 3 3843
Fragments arranged by size:
3843 2069 831 3
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 616 648 616
Size: 32 6714
AlwNI CAG_nnn'CTG
Cuts at: 731 1392 2058 2477 3084 3504 4051 5489 731
Size: 661 666 419 607 420 547 1438 1988
Fragments arranged by size:
1988 1438 666 661 607 547 420 419
ApaI G_GGCC'C
Cuts at: 189 189
Size: 6746
ApaLI G'TGCA_C
Cuts at: 3216 3570 5584 3216
Size: 354 2014 4378
Fragments arranged by size:
4378 2014 354
ApoI r'AATT_y
Cuts at: 2850 2925 3750 6099 6355 2850
Size: 75 825 2349 256 3241
Fragments arranged by size:
3241 2349 825 256 75
AseI AT'TA_AT
Cuts at: 20 20
Size: 6746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6746
AvaI C'yCGr_G
Cuts at: 189 1994 2261 3442 3766 189
Size: 1805 267 1181 324 3169
Fragments arranged by size:
3169 1805 1181 324 267
BamHI G'GATC_C
Cuts at: 3771 3771
Size: 6746
BanI G'GyrC_C
Cuts at: 224 1195 1303 1675 1774 2402 2506 2611 3762
Size: 971 108 372 99 628 104 105 1151
Cuts at: 3762 4176 4424 4459 224
Size: 414 248 35 2511
Fragments arranged by size:
2511 1151 971 628 414 372 248 108
105 104 99 35
BanII G_rGCy'C
Cuts at: 189 1763 2065 2450 3438 3760 4790 189
Size: 1574 302 385 988 322 1030 2145
Fragments arranged by size:
2145 1574 1030 988 385 322 302
BbeI G_GCGC'C
Cuts at: 1778 2510 4180 4428 1778
Size: 732 1670 248 4096
Fragments arranged by size:
4096 1670 732 248
BbsI GAAGACnn'nnnn_
Cuts at: 521 927 932 1469 2590 3619 521
Size: 406 5 537 1121 1029 3648
Fragments arranged by size:
3648 1121 1029 537 406 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 564 598 90
Size: 34 440 34 6238
Fragments arranged by size:
6238 440 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3347 4644 5700 6746 3347
Size: 1297 1056 1046 3347
Fragments arranged by size:
3347 1297 1056 1046
BclI T'GATC_A
Cuts at: 872 2903 4266 872
Size: 2031 1363 3352
Fragments arranged by size:
3352 2031 1363
BfrBI ATG'CAT
Cuts at: 3326 3326
Size: 6746
BglI GCCn_nnn'nGGC
Cuts at: 149 193 406 149
Size: 44 213 6489
Fragments arranged by size:
6489 213 44
BglII A'GATC_T
Cuts at: 1002 1014 1134 4261 6298 1002
Size: 12 120 3127 2037 1450
Fragments arranged by size:
3127 2037 1450 120 12
BlpI GC'TnA_GC
Cuts at: 2825 3858 2825
Size: 1033 5713
Bme1580I G_kGCm'C
Cuts at: 189 1200 1680 3220 3574 4371 4464 5588 189
Size: 1011 480 1540 354 797 93 1124 1347
Fragments arranged by size:
1540 1347 1124 1011 797 480 354 93
BmgBI CAC'GTC
Cuts at: 930 2484 930
Size: 1554 5192
BmrI ACTGGGnnnn_n'
Cuts at: 1201 3958 4039 4374 6518 1201
Size: 2757 81 335 2144 1429
Fragments arranged by size:
2757 2144 1429 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1153 5113 1153
Size: 3960 2786
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 531 631 1057 1122 1652 1942 2087 2179 2194
Size: 100 426 65 530 290 145 92 15
Cuts at: 2194 2353 2623 531
Size: 159 270 4654
Fragments arranged by size:
4654 530 426 290 270 159 145 100
92 65 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3922 4801 5515 5813 3922
Size: 879 714 298 4855
Fragments arranged by size:
4855 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1991 6450 1991
Size: 4459 2287
BsaAI yAC'GTr
Cuts at: 2658 4099 2658
Size: 1441 5305
BsaBI GATnn'nnATC
Cuts at: 6 560 4260 6297 6
Size: 554 3700 2037 455
Fragments arranged by size:
3700 2037 554 455
BsaHI Gr'CG_yC
Cuts at: 233 1775 1867 2507 3227 3624 4177 4425 5127
Size: 1542 92 640 720 397 553 248 702
Cuts at: 5127 6623 233
Size: 1496 356
Fragments arranged by size:
1542 1496 720 702 640 553 397 356
248 92
BsaWI w'CCGG_w
Cuts at: 1899 1971 3425 3935 4456 5134 5545 5692 5966
Size: 72 1454 510 521 678 411 147 274
Cuts at: 5966 1899
Size: 2679
Fragments arranged by size:
2679 1454 678 521 510 411 274 147
72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3011 3041 3526 3556 3011
Size: 30 485 30 6201
Fragments arranged by size:
6201 485 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 670 772 1145 2279 670
Size: 102 373 1134 5137
Fragments arranged by size:
5137 1134 373 102
BseYI C'CCAG_C
Cuts at: 962 2010 2051 2058 2381 3074 4171 4929 5594
Size: 1048 41 7 323 693 1097 758 665
Cuts at: 5594 6002 962
Size: 408 1706
Fragments arranged by size:
1706 1097 1048 758 693 665 408 323
41 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 812 1198 1480 1858 2095 2404 2947 3247
Size: 617 386 282 378 237 309 543 300
Cuts at: 3247 195
Size: 3694
Fragments arranged by size:
3694 617 543 386 378 309 300 282
237
BsiEI CG_ry'CG
Cuts at: 104 132 2045 3183 3430 4288 4334 5564 104
Size: 28 1913 1138 247 858 46 1230 1286
Fragments arranged by size:
1913 1286 1230 1138 858 247 46 28
BsiHKAI G_wGCw'C
Cuts at: 477 1763 2165 3220 3574 3760 4538 4728 5588
Size: 1286 402 1055 354 186 778 190 860
Cuts at: 5588 477
Size: 1635
Fragments arranged by size:
1635 1286 1055 860 778 402 354 190
186
BsmI GAATG_Cn'
Cuts at: 771 3297 3328 771
Size: 2526 31 4189
Fragments arranged by size:
4189 2526 31
Bsp1286I G_dGCh'C
Cuts at: 189 477 1200 1680 1763 2065 2165 2450 3220
Size: 288 723 480 83 302 100 285 770
Cuts at: 3220 3438 3574 3760 4371 4464 4538 4728 4790
Size: 218 136 186 611 93 74 190 62
Cuts at: 4790 5588 189
Size: 798 1347
Fragments arranged by size:
1347 798 770 723 611 480 302 288
285 218 190 186 136 100 93 83
74 62
BspEI T'CCGG_A
Cuts at: 1899 1971 1899
Size: 72 6674
BspHI T'CATG_A
Cuts at: 5178 5178
Size: 6746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 143 1796 2820 3092 3796 4010 4312 4693 143
Size: 1653 1024 272 704 214 302 381 2196
Fragments arranged by size:
2196 1653 1024 704 381 302 272 214
BsrBI CCG'CTC
Cuts at: 50 5038 5092 6576 6741 50
Size: 4988 54 1484 165 55
Fragments arranged by size:
4988 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4658 4658
Size: 6746
BsrFI r'CCGG_y
Cuts at: 131 163 218 3182 4744 4925 131
Size: 32 55 2964 1562 181 1952
Fragments arranged by size:
2964 1952 1562 181 55 32
BsrGI T'GTAC_A
Cuts at: 1229 1229
Size: 6746
BssSI C'ACGA_G
Cuts at: 1429 5017 5725 1429
Size: 3588 708 2450
Fragments arranged by size:
3588 2450 708
BstAPI GCAn_nnn'nTGC
Cuts at: 273 2632 3084 4123 273
Size: 2359 452 1039 2896
Fragments arranged by size:
2896 2359 1039 452
BstBI TT'CG_AA
Cuts at: 1502 5107 1502
Size: 3605 3141
BstXI CCAn_nnnn'nTGG
Cuts at: 915 970 1056 2658 915
Size: 55 86 1602 5003
Fragments arranged by size:
5003 1602 86 55
BstYI r'GATC_y
Cuts at: 1 593 1002 1014 1134 3771 4238 4261 4596
Size: 592 409 12 120 2637 467 23 335
Cuts at: 4596 4842 5148 5160 5246 6298 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2637 1052 592 467 449 409 335 306
246 120 86 23 12 12
Bsu36I CC'TnA_GG
Cuts at: 566 682 823 566
Size: 116 141 6489
Fragments arranged by size:
6489 141 116
BtgI C'CryG_G
Cuts at: 118 180 1514 3134 3541 118
Size: 62 1334 1620 407 3323
Fragments arranged by size:
3323 1620 1334 407 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2325 3031 4696 4877 5040 2325
Size: 706 1665 181 163 4031
Fragments arranged by size:
4031 1665 706 181 163
BtsI GCAGTG_nn'
Cuts at: 507 1210 1402 1463 1870 2478 3259 3572 3615
Size: 703 192 61 407 608 781 313 43
Cuts at: 3615 3989 507
Size: 374 3264
Fragments arranged by size:
3264 781 703 608 407 374 313 192
61 43
DraI TTT'AAA
Cuts at: 3745 3745
Size: 6746
DraIII CAC_nnn'GTG
Cuts at: 1195 1221 1727 1195
Size: 26 506 6214
Fragments arranged by size:
6214 506 26
DrdI GACnn_nn'nnGTC
Cuts at: 4452 5796 4452
Size: 1344 5402
EaeI y'GGCC_r
Cuts at: 129 251 398 1361 1742 2117 2216 2660 3180
Size: 122 147 963 381 375 99 444 520
Cuts at: 3180 3299 4331 4505 4896 4923 6268 129
Size: 119 1032 174 391 27 1345 607
Fragments arranged by size:
1345 1032 963 607 520 444 391 381
375 174 147 122 119 99 27
EagI C'GGCC_G
Cuts at: 129 3180 4331 129
Size: 3051 1151 2544
Fragments arranged by size:
3051 2544 1151
EarI CTCTTCn'nnn_
Cuts at: 1946 2155 2522 2576 3164 3319 4769 4979 1946
Size: 209 367 54 588 155 1450 210 3713
Fragments arranged by size:
3713 1450 588 367 210 209 155 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 331 1861 4954 5696 5842 6556 331
Size: 1530 3093 742 146 714 521
Fragments arranged by size:
3093 1530 742 714 521 146
EcoICRI GAG'CTC
Cuts at: 1761 3758 1761
Size: 1997 4749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 531 631 1057 1122 1652 1942 1962 2087
Size: 68 100 426 65 530 290 20 125
Cuts at: 2087 2179 2194 2353 2392 2623 4571 5003 5356
Size: 92 15 159 39 231 1948 432 353
Cuts at: 5356 6018 463
Size: 662 1191
Fragments arranged by size:
1948 1191 662 530 432 426 353 290
231 159 125 100 92 68 65 39
20 15
EcoNI CCTnn'n_nnAGG
Cuts at: 826 1294 826
Size: 468 6278
EcoO109I rG'GnC_Cy
Cuts at: 155 185 186 1169 1287 2392 3081 3885 155
Size: 30 1 983 118 1105 689 804 3016
Fragments arranged by size:
3016 1105 983 804 689 118 30 1
EcoRI G'AATT_C
Cuts at: 2925 3750 2925
Size: 825 5921
EcoRV GAT'ATC
Cuts at: 2715 2715
Size: 6746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4765 4765
Size: 6746
FspI TGC'GCA
Cuts at: 350 4527 350
Size: 4177 2569
HaeII r_GCGC'y
Cuts at: 333 854 1778 2510 4180 4428 5658 6429 333
Size: 521 924 732 1670 248 1230 771 650
Fragments arranged by size:
1670 1230 924 771 732 650 521 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1246 1278 1813 1845 1894 1926 2060 2074 2092
Size: 32 535 32 49 32 134 14 18
Cuts at: 2092 2106 2329 2361 3455 3487 3511 3543 4588
Size: 14 223 32 1094 32 24 32 1045
Cuts at: 4588 4618 4620 4650 1246
Size: 30 2 30 3342
Fragments arranged by size:
3342 1094 1045 535 223 134 49 32
32 32 32 32 32 30 30 24
18 14 14 2
HincII GTy'rAC
Cuts at: 1179 1686 2954 3468 3785 1179
Size: 507 1268 514 317 4140
Fragments arranged by size:
4140 1268 514 507 317
KasI G'GCGC_C
Cuts at: 1774 2506 4176 4424 1774
Size: 732 1670 248 4096
Fragments arranged by size:
4096 1670 732 248
KpnI G_GTAC'C
Cuts at: 3766 3766
Size: 6746
MluI A'CGCG_T
Cuts at: 6179 6179
Size: 6746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 239 524 2072 3534 5505 5689 239
Size: 285 1548 1462 1971 184 1296
Fragments arranged by size:
1971 1548 1462 1296 285 184
MscI TGG'CCA
Cuts at: 253 400 1744 2119 2218 2662 4507 253
Size: 147 1344 375 99 444 1845 2492
Fragments arranged by size:
2492 1845 1344 444 375 147 99
MslI CAynn'nnrTG
Cuts at: 470 778 913 1901 2278 2366 2656 2723 2918
Size: 308 135 988 377 88 290 67 195
Cuts at: 2918 4735 4862 470
Size: 1817 127 2354
Fragments arranged by size:
2354 1817 988 377 308 290 195 135
127 88 67
MspA1I CmG'CkG
Cuts at: 120 182 509 1389 1623 1630 2055 2127 2214
Size: 62 327 880 234 7 425 72 87
Cuts at: 2214 2307 2694 3298 3857 4171 4531 5315 5560
Size: 93 387 604 559 314 360 784 245
Cuts at: 5560 120
Size: 1306
Fragments arranged by size:
1306 880 784 604 559 425 387 360
327 314 245 234 93 87 72 62
7
NaeI GCC'GGC
Cuts at: 133 220 3184 4927 133
Size: 87 2964 1743 1952
Fragments arranged by size:
2964 1952 1743 87
NarI GG'CG_CC
Cuts at: 1775 2507 4177 4425 1775
Size: 732 1670 248 4096
Fragments arranged by size:
4096 1670 732 248
NdeI CA'TA_TG
Cuts at: 2254 2254
Size: 6746
NgoMIV G'CCGG_C
Cuts at: 131 218 3182 4925 131
Size: 87 2964 1743 1952
Fragments arranged by size:
2964 1952 1743 87
NsiI A_TGCA'T
Cuts at: 3328 3328
Size: 6746
NspI r_CATG'y
Cuts at: 1320 1351 2037 2277 2381 3330 3799 4299 5902
Size: 31 686 240 104 949 469 500 1603
Cuts at: 5902 1320
Size: 2164
Fragments arranged by size:
2164 1603 949 686 500 469 240 104
31
PasI CC'CwG_GG
Cuts at: 970 970
Size: 6746
PciI A'CATG_T
Cuts at: 1347 2273 5898 1347
Size: 926 3625 2195
Fragments arranged by size:
3625 2195 926
PflMI CCAn_nnn'nTGG
Cuts at: 2058 2381 2058
Size: 323 6423
PfoI T'CCnGG_A
Cuts at: 1919 2388 2936 1919
Size: 469 548 5729
Fragments arranged by size:
5729 548 469
PmeI GTTT'AAAC
Cuts at: 3745 3745
Size: 6746
PmlI CAC'GTG
Cuts at: 2658 2658
Size: 6746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 598 630 5192 5224 598
Size: 32 4562 32 2120
Fragments arranged by size:
4562 2120 32 32
PpuMI rG'GwC_Cy
Cuts at: 155 2392 3081 155
Size: 2237 689 3820
Fragments arranged by size:
3820 2237 689
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6746
PspOMI G'GGCC_C
Cuts at: 185 185
Size: 6746
PstI C_TGCA'G
Cuts at: 305 1298 1397 1769 1793 1865 3793 4478 305
Size: 993 99 372 24 72 1928 685 2573
Fragments arranged by size:
2573 1928 993 685 372 99 72 24
PvuI CG_AT'CG
Cuts at: 104 2045 104
Size: 1941 4805
PvuII CAG'CTG
Cuts at: 509 1389 1630 2055 2214 2694 4171 4531 509
Size: 880 241 425 159 480 1477 360 2724
Fragments arranged by size:
2724 1477 880 480 425 360 241 159
RsrII CG'GwC_CG
Cuts at: 4941 4941
Size: 6746
SacI G_AGCT'C
Cuts at: 1763 3760 1763
Size: 1997 4749
SacII CC_GC'GG
Cuts at: 121 183 121
Size: 62 6684
SalI G'TCGA_C
Cuts at: 3783 3783
Size: 6746
SapI GCTCTTCn'nnn_
Cuts at: 1946 3164 4769 4979 1946
Size: 1218 1605 210 3713
Fragments arranged by size:
3713 1605 1218 210
SbfI CC_TGCA'GG
Cuts at: 3793 3793
Size: 6746
ScaI AGT'ACT
Cuts at: 6263 6263
Size: 6746
SexAI A'CCwGG_T
Cuts at: 2394 3482 2394
Size: 1088 5658
SfcI C'TryA_G
Cuts at: 33 301 673 1294 1393 1618 1765 1789 1861
Size: 268 372 621 99 225 147 24 72
Cuts at: 1861 3789 4474 5442 5633 33
Size: 1928 685 968 191 1146
Fragments arranged by size:
1928 1146 968 685 621 372 268 225
191 147 99 72 24
SfoI GGC'GCC
Cuts at: 1776 2508 4178 4426 1776
Size: 732 1670 248 4096
Fragments arranged by size:
4096 1670 732 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6746
SgrAI Cr'CCGG_yG
Cuts at: 163 163
Size: 6746
SmaI CCC'GGG
Cuts at: 3768 3768
Size: 6746
SmlI C'TyrA_G
Cuts at: 3901 4816 5530 5792 3901
Size: 915 714 262 4855
Fragments arranged by size:
4855 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6746
SphI G_CATG'C
Cuts at: 2037 3330 3799 4299 2037
Size: 1293 469 500 4484
Fragments arranged by size:
4484 1293 500 469
StyI C'CwwG_G
Cuts at: 3530 3650 3880 4234 3530
Size: 120 230 354 6042
Fragments arranged by size:
6042 354 230 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1183 2390 3718 4329 4462 4921 5130 1183
Size: 1207 1328 611 133 459 209 2799
Fragments arranged by size:
2799 1328 1207 611 459 209 133
TatI w'GTAC_w
Cuts at: 431 1229 2589 3717 6261 431
Size: 798 1360 1128 2544 916
Fragments arranged by size:
2544 1360 1128 916 798
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 233 265 3011 3043 3526 3558 6263 6295 233
Size: 32 2746 32 483 32 2705 32 684
Fragments arranged by size:
2746 2705 684 483 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 931 1852 4543 931
Size: 921 2691 3134
Fragments arranged by size:
3134 2691 921
XbaI T'CTAG_A
Cuts at: 3777 3777
Size: 6746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 540 4241 540
Size: 3701 3045
XmaI C'CCGG_G
Cuts at: 3766 3766
Size: 6746
XmnI GAAnn'nnTTC
Cuts at: 1498 1498
Size: 6746
ZraI GAC'GTC
Cuts at: 3625 3625
Size: 6746
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AscI AvrII BaeI BbvCI BmtI Bpu10I
BsiWI BsmBI BssHII BstEII BstZ17I ClaI CspCI FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
PacI PshAI PspXI PsrI SanDI SfiI SnaBI SrfI
SspI StuI SwaI XhoI