Restriction map for
pF1KA1261
(Circular) (Six-base) MAPSORT of: pF1KA1261.seq Check: 4584 from: 1 to: 5432
pF1KA1261 5432 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 453 453
Size: 5432
AccI GT'mk_AC
Cuts at: 2470 2470
Size: 5432
Acc65I G'GTAC_C
Cuts at: 2448 2448
Size: 5432
AclI AA'CG_TT
Cuts at: 5272 5272
Size: 5432
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 548 707 830 3257 3689 4042 4704 274
Size: 274 159 123 2427 432 353 662 1002
Fragments arranged by size:
2427 1002 662 432 353 274 159 123
AflIII A'CryG_T
Cuts at: 1608 1610 2784 4584 4865 1608
Size: 2 1174 1800 281 2175
Fragments arranged by size:
2175 1800 1174 281 2
AleI CACnn'nnGTG
Cuts at: 1397 1397
Size: 5432
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 182 182
Size: 5432
AlwNI CAG_nnn'CTG
Cuts at: 1327 2042 2737 4175 1327
Size: 715 695 1438 2584
Fragments arranged by size:
2584 1438 715 695
ApaLI G'TGCA_C
Cuts at: 392 4270 392
Size: 3878 1554
ApoI r'AATT_y
Cuts at: 181 2436 4785 5041 181
Size: 2255 2349 256 572
Fragments arranged by size:
2349 2255 572 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5432
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5432
AvaI C'yCGr_G
Cuts at: 905 2452 905
Size: 1547 3885
BamHI G'GATC_C
Cuts at: 891 2457 891
Size: 1566 3866
BanI G'GyrC_C
Cuts at: 153 1999 2448 2862 3110 3145 153
Size: 1846 449 414 248 35 2440
Fragments arranged by size:
2440 1846 449 414 248 35
BanII G_rGCy'C
Cuts at: 572 721 916 1295 2446 3476 572
Size: 149 195 379 1151 1030 2528
Fragments arranged by size:
2528 1151 1030 379 195 149
BbeI G_GCGC'C
Cuts at: 2866 3114 2866
Size: 248 5184
BbsI GAAGACnn'nnnn_
Cuts at: 292 292
Size: 5432
BciVI GTATCCnnnnn_n'
Cuts at: 738 3330 4386 5432 738
Size: 2592 1056 1046 738
Fragments arranged by size:
2592 1056 1046 738
BclI T'GATC_A
Cuts at: 687 2952 687
Size: 2265 3167
BglI GCCn_nnn'nGGC
Cuts at: 1661 1661
Size: 5432
BglII A'GATC_T
Cuts at: 2093 2947 4984 2093
Size: 854 2037 2541
Fragments arranged by size:
2541 2037 854
BlpI GC'TnA_GC
Cuts at: 2344 2544 2344
Size: 200 5232
Bme1580I G_kGCm'C
Cuts at: 396 1241 3057 3150 4274 396
Size: 845 1816 93 1124 1554
Fragments arranged by size:
1816 1554 1124 845 93
BmrI ACTGGGnnnn_n'
Cuts at: 2644 2725 3060 5204 2644
Size: 81 335 2144 2872
Fragments arranged by size:
2872 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3799 3799
Size: 5432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 556 609 2141 2310 490
Size: 66 53 1532 169 3612
Fragments arranged by size:
3612 1532 169 66 53
Bpu10I CC'TnA_GC
Cuts at: 121 121
Size: 5432
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1164 2212 2608 3487 4201 4499 946
Size: 218 1048 396 879 714 298 1879
Fragments arranged by size:
1879 1048 879 714 396 298 218
BsaI GGTCTCn'nnnn_
Cuts at: 541 5136 541
Size: 4595 837
BsaAI yAC'GTr
Cuts at: 1611 2785 1611
Size: 1174 4258
BsaBI GATnn'nnATC
Cuts at: 6 2946 4983 6
Size: 2940 2037 455
Fragments arranged by size:
2940 2037 455
BsaHI Gr'CG_yC
Cuts at: 1508 2863 3111 3813 5309 1508
Size: 1355 248 702 1496 1631
Fragments arranged by size:
1631 1496 1355 702 248
BsaWI w'CCGG_w
Cuts at: 2621 3142 3820 4231 4378 4652 2621
Size: 521 678 411 147 274 3401
Fragments arranged by size:
3401 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 521 551 691 721 754 784 1071 1101 1288
Size: 30 140 30 33 30 287 30 187
Cuts at: 1288 1318 1561 1591 2071 2101 521
Size: 30 243 30 480 30 3852
Fragments arranged by size:
3852 480 287 243 187 140 33 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 774 1008 774
Size: 234 5198
BseYI C'CCAG_C
Cuts at: 512 915 1288 1682 2857 3615 4280 4688 512
Size: 403 373 394 1175 758 665 408 1256
Fragments arranged by size:
1256 1175 758 665 408 403 394 373
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 1909 460
Size: 1449 3983
BsiEI CG_ry'CG
Cuts at: 104 2974 3020 4250 104
Size: 2870 46 1230 1286
Fragments arranged by size:
2870 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 396 425 633 721 2446 3224 3414 4274 396
Size: 29 208 88 1725 778 190 860 1554
Fragments arranged by size:
1725 1554 860 778 208 190 88 29
BsmI GAATG_Cn'
Cuts at: 387 1430 2289 387
Size: 1043 859 3530
Fragments arranged by size:
3530 1043 859
BsmBI CGTCTCn'nnnn_
Cuts at: 1071 1071
Size: 5432
Bsp1286I G_dGCh'C
Cuts at: 396 425 572 633 721 916 1241 1295 2446
Size: 29 147 61 88 195 325 54 1151
Cuts at: 2446 3057 3150 3224 3414 3476 4274 396
Size: 611 93 74 190 62 798 1554
Fragments arranged by size:
1554 1151 798 611 325 195 190 147
93 88 74 62 61 54 29
BspHI T'CATG_A
Cuts at: 2205 3864 2205
Size: 1659 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 453 2057 2482 2696 2998 3379 453
Size: 1604 425 214 302 381 2506
Fragments arranged by size:
2506 1604 425 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1279 3724 3778 5262 5427 50
Size: 1229 2445 54 1484 165 55
Fragments arranged by size:
2445 1484 1229 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2160 3344 2160
Size: 1184 4248
BsrFI r'CCGG_y
Cuts at: 3430 3611 3430
Size: 181 5251
BsrGI T'GTAC_A
Cuts at: 1613 1613
Size: 5432
BssSI C'ACGA_G
Cuts at: 1602 3703 4411 1602
Size: 2101 708 2623
Fragments arranged by size:
2623 2101 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1327 2809 1327
Size: 1482 3950
BstBI TT'CG_AA
Cuts at: 3793 3793
Size: 5432
BstEII G'GTnAC_C
Cuts at: 465 1584 465
Size: 1119 4313
BstXI CCAn_nnnn'nTGG
Cuts at: 1966 1966
Size: 5432
BstYI r'GATC_y
Cuts at: 1 891 1920 2093 2457 2924 2947 3282 3528
Size: 890 1029 173 364 467 23 335 246
Cuts at: 3528 3834 3846 3932 4984 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 1029 890 467 449 364 335 306
246 173 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1186 1186
Size: 5432
BtgI C'CryG_G
Cuts at: 470 1563 2360 470
Size: 1093 797 3542
Fragments arranged by size:
3542 1093 797
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 857 1893 3382 3563 3726 857
Size: 1036 1489 181 163 2563
Fragments arranged by size:
2563 1489 1036 181 163
BtsI GCAGTG_nn'
Cuts at: 609 2141 2675 609
Size: 1532 534 3366
Fragments arranged by size:
3366 1532 534
DraI TTT'AAA
Cuts at: 180 2431 180
Size: 2251 3181
DrdI GACnn_nn'nnGTC
Cuts at: 2032 3138 4482 2032
Size: 1106 1344 2982
Fragments arranged by size:
2982 1344 1106
EaeI y'GGCC_r
Cuts at: 1856 3017 3191 3582 3609 4954 1856
Size: 1161 174 391 27 1345 2334
Fragments arranged by size:
2334 1345 1161 391 174 27
EagI C'GGCC_G
Cuts at: 3017 3017
Size: 5432
EarI CTCTTCn'nnn_
Cuts at: 212 726 3455 3665 212
Size: 514 2729 210 1979
Fragments arranged by size:
2729 1979 514 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3640 4382 4528 5242 3640
Size: 742 146 714 3830
Fragments arranged by size:
3830 742 714 146
EcoICRI GAG'CTC
Cuts at: 719 2444 719
Size: 1725 3707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 490 548 556 609 707 830 2141 2310
Size: 216 58 8 53 98 123 1311 169
Cuts at: 2310 3257 3689 4042 4704 274
Size: 947 432 353 662 1002
Fragments arranged by size:
1311 1002 947 662 432 353 216 169
123 98 58 53 8
EcoNI CCTnn'n_nnAGG
Cuts at: 1941 2053 1941
Size: 112 5320
EcoO109I rG'GnC_Cy
Cuts at: 1190 1788 2039 2046 2571 1190
Size: 598 251 7 525 4051
Fragments arranged by size:
4051 598 525 251 7
EcoRI G'AATT_C
Cuts at: 2436 2436
Size: 5432
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1899 3451 1899
Size: 1552 3880
FspI TGC'GCA
Cuts at: 3213 3213
Size: 5432
HaeII r_GCGC'y
Cuts at: 2866 3114 4344 5115 2866
Size: 248 1230 771 3183
Fragments arranged by size:
3183 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 521 553 691 695 723 727 769 801 880
Size: 32 138 4 28 4 42 32 79
Cuts at: 880 912 1511 1543 1813 1845 2071 2085 2103
Size: 32 599 32 270 32 226 14 18
Cuts at: 2103 2117 3274 3304 3306 3336 521
Size: 14 1157 30 2 30 2617
Fragments arranged by size:
2617 1157 599 270 226 138 79 42
32 32 32 32 32 30 30 28
18 14 14 4 4 2
HincII GTy'rAC
Cuts at: 876 1167 2471 876
Size: 291 1304 3837
Fragments arranged by size:
3837 1304 291
KasI G'GCGC_C
Cuts at: 2862 3110 2862
Size: 248 5184
KpnI G_GTAC'C
Cuts at: 2452 2452
Size: 5432
MluI A'CGCG_T
Cuts at: 4865 4865
Size: 5432
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1243 1737 1825 4191 4375 1243
Size: 494 88 2366 184 2300
Fragments arranged by size:
2366 2300 494 184 88
MscI TGG'CCA
Cuts at: 1858 3193 1858
Size: 1335 4097
MslI CAynn'nnrTG
Cuts at: 530 683 1397 1399 1912 1964 2204 2359 3421
Size: 153 714 2 513 52 240 155 1062
Cuts at: 3421 3548 530
Size: 127 2414
Fragments arranged by size:
2414 1062 714 513 240 155 153 127
52 2
MspA1I CmG'CkG
Cuts at: 300 1324 1339 1976 2067 2543 2857 3217 4001
Size: 1024 15 637 91 476 314 360 784
Cuts at: 4001 4246 300
Size: 245 1486
Fragments arranged by size:
1486 1024 784 637 476 360 314 245
91 15
NaeI GCC'GGC
Cuts at: 3613 3613
Size: 5432
NarI GG'CG_CC
Cuts at: 2863 3111 2863
Size: 248 5184
NcoI C'CATG_G
Cuts at: 470 470
Size: 5432
NgoMIV G'CCGG_C
Cuts at: 3611 3611
Size: 5432
NruI TCG'CGA
Cuts at: 116 116
Size: 5432
NspI r_CATG'y
Cuts at: 1398 1540 2173 2485 2985 4588 1398
Size: 142 633 312 500 1603 2242
Fragments arranged by size:
2242 1603 633 500 312 142
PciI A'CATG_T
Cuts at: 4584 4584
Size: 5432
PflMI CCAn_nnn'nTGG
Cuts at: 1938 2009 1938
Size: 71 5361
PfoI T'CCnGG_A
Cuts at: 1128 1434 1128
Size: 306 5126
PmeI GTTT'AAAC
Cuts at: 2431 2431
Size: 5432
PmlI CAC'GTG
Cuts at: 1611 1611
Size: 5432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 3878
Size: 32 5400
PpuMI rG'GwC_Cy
Cuts at: 1190 1788 2039 2046 1190
Size: 598 251 7 4576
Fragments arranged by size:
4576 598 251 7
PspXI vC'TCGA_Gb
Cuts at: 905 905
Size: 5432
PstI C_TGCA'G
Cuts at: 1726 1897 2072 2479 3164 1726
Size: 171 175 407 685 3994
Fragments arranged by size:
3994 685 407 175 171
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5432
PvuII CAG'CTG
Cuts at: 1324 1976 2067 2857 3217 1324
Size: 652 91 790 360 3539
Fragments arranged by size:
3539 790 652 360 91
RsrII CG'GwC_CG
Cuts at: 3627 3627
Size: 5432
SacI G_AGCT'C
Cuts at: 721 2446 721
Size: 1725 3707
SalI G'TCGA_C
Cuts at: 2469 2469
Size: 5432
SapI GCTCTTCn'nnn_
Cuts at: 726 3455 3665 726
Size: 2729 210 2493
Fragments arranged by size:
2729 2493 210
SbfI CC_TGCA'GG
Cuts at: 2479 2479
Size: 5432
ScaI AGT'ACT
Cuts at: 1011 1773 4949 1011
Size: 762 3176 1494
Fragments arranged by size:
3176 1494 762
SfcI C'TryA_G
Cuts at: 33 1722 1893 2068 2475 3160 4128 4319 33
Size: 1689 171 175 407 685 968 191 1146
Fragments arranged by size:
1689 1146 968 685 407 191 175 171
SfoI GGC'GCC
Cuts at: 2864 3112 2864
Size: 248 5184
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5432
SmaI CCC'GGG
Cuts at: 2454 2454
Size: 5432
SmlI C'TyrA_G
Cuts at: 905 961 1179 2227 2587 3502 4216 4478 905
Size: 56 218 1048 360 915 714 262 1859
Fragments arranged by size:
1859 1048 915 714 360 262 218 56
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5432
SphI G_CATG'C
Cuts at: 1540 2485 2985 1540
Size: 945 500 3987
Fragments arranged by size:
3987 945 500
StuI AGG'CCT
Cuts at: 1140 1140
Size: 5432
StyI C'CwwG_G
Cuts at: 470 1874 1937 2566 2920 470
Size: 1404 63 629 354 2982
Fragments arranged by size:
2982 1404 629 354 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 606 1673 3015 3148 3607 3816 606
Size: 1067 1342 133 459 209 2222
Fragments arranged by size:
2222 1342 1067 459 209 133
TatI w'GTAC_w
Cuts at: 1009 1613 1771 4947 1009
Size: 604 158 3176 1494
Fragments arranged by size:
3176 1494 604 158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 754 786 1288 1320 1561 1593 4949 4981 754
Size: 32 502 32 241 32 3356 32 1205
Fragments arranged by size:
3356 1205 502 241 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 533 3229 533
Size: 2696 2736
XbaI T'CTAG_A
Cuts at: 2463 2463
Size: 5432
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1788 2123 2927 1788
Size: 335 804 4293
Fragments arranged by size:
4293 804 335
XhoI C'TCGA_G
Cuts at: 905 905
Size: 5432
XmaI C'CCGG_G
Cuts at: 2452 2452
Size: 5432
XmnI GAAnn'nnTTC
Cuts at: 221 221
Size: 5432
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BspEI BssHII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PasI PshAI PsiI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI