Restriction map for
pF1KA1270
(Circular) (Six-base) MAPSORT of: pF1KA1270.seq Check: 3533 from: 1 to: 6068
pF1KA1270 6068 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2024 2024
Size: 6068
AccI GT'mk_AC
Cuts at: 1723 3106 1723
Size: 1383 4685
Acc65I G'GTAC_C
Cuts at: 1233 3084 1233
Size: 1851 4217
AclI AA'CG_TT
Cuts at: 5908 5908
Size: 6068
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 1430 1808 3893 4325 4678 5340 1376
Size: 54 378 2085 432 353 662 2104
Fragments arranged by size:
2104 2085 662 432 378 353 54
AfeI AGC'GCT
Cuts at: 1768 1768
Size: 6068
AflIII A'CryG_T
Cuts at: 408 3420 5220 5501 408
Size: 3012 1800 281 975
Fragments arranged by size:
3012 1800 975 281
AleI CACnn'nnGTG
Cuts at: 2839 2839
Size: 6068
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2684 2857 2684
Size: 173 5895
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1852 1884 1852
Size: 32 6036
AlwNI CAG_nnn'CTG
Cuts at: 882 1106 2043 2266 2493 2727 2773 3373 4811
Size: 224 937 223 227 234 46 600 1438
Cuts at: 4811 882
Size: 2139
Fragments arranged by size:
2139 1438 937 600 234 227 224 223
46
ApaI G_GGCC'C
Cuts at: 767 1887 2050 767
Size: 1120 163 4785
Fragments arranged by size:
4785 1120 163
ApaLI G'TGCA_C
Cuts at: 4906 4906
Size: 6068
ApoI r'AATT_y
Cuts at: 3072 5421 5677 3072
Size: 2349 256 3463
Fragments arranged by size:
3463 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6068
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6068
AvaI C'yCGr_G
Cuts at: 1880 2474 3088 1880
Size: 594 614 4860
Fragments arranged by size:
4860 614 594
AvrII C'CTAG_G
Cuts at: 1221 1221
Size: 6068
BamHI G'GATC_C
Cuts at: 378 590 1163 3093 378
Size: 212 573 1930 3353
Fragments arranged by size:
3353 1930 573 212
BanI G'GyrC_C
Cuts at: 282 370 690 1011 1206 1233 1693 2202 2289
Size: 88 320 321 195 27 460 509 87
Cuts at: 2289 2902 2995 3084 3498 3746 3781 282
Size: 613 93 89 414 248 35 2569
Fragments arranged by size:
2569 613 509 460 414 321 320 248
195 93 89 88 87 35 27
BanII G_rGCy'C
Cuts at: 209 767 810 1887 2050 2071 2977 3082 4112
Size: 558 43 1077 163 21 906 105 1030
Cuts at: 4112 209
Size: 2165
Fragments arranged by size:
2165 1077 1030 906 558 163 105 43
21
BbeI G_GCGC'C
Cuts at: 3502 3750 3502
Size: 248 5820
BbsI GAAGACnn'nnnn_
Cuts at: 831 1116 831
Size: 285 5783
BbvCI CC'TCA_GC
Cuts at: 181 3052 181
Size: 2871 3197
BciVI GTATCCnnnnn_n'
Cuts at: 741 3966 5022 6068 741
Size: 3225 1056 1046 741
Fragments arranged by size:
3225 1056 1046 741
BclI T'GATC_A
Cuts at: 3588 3588
Size: 6068
BfrBI ATG'CAT
Cuts at: 2305 2305
Size: 6068
BglI GCCn_nnn'nGGC
Cuts at: 171 235 891 1333 2470 2622 171
Size: 64 656 442 1137 152 3617
Fragments arranged by size:
3617 1137 656 442 152 64
BglII A'GATC_T
Cuts at: 1196 3583 5620 1196
Size: 2387 2037 1644
Fragments arranged by size:
2387 2037 1644
BlpI GC'TnA_GC
Cuts at: 679 1559 3180 679
Size: 880 1621 3567
Fragments arranged by size:
3567 1621 880
Bme1580I G_kGCm'C
Cuts at: 287 373 767 1014 1551 1631 1887 2031 2050
Size: 86 394 247 537 80 256 144 19
Cuts at: 2050 2207 2294 2431 2937 3693 3786 4910 287
Size: 157 87 137 506 756 93 1124 1445
Fragments arranged by size:
1445 1124 756 537 506 394 256 247
157 144 137 93 87 86 80 19
BmgBI CAC'GTC
Cuts at: 409 1863 2837 409
Size: 1454 974 3640
Fragments arranged by size:
3640 1454 974
BmrI ACTGGGnnnn_n'
Cuts at: 311 1264 1867 2034 2387 2462 2613 3280 3361
Size: 953 603 167 353 75 151 667 81
Cuts at: 3361 3696 5840 311
Size: 335 2144 539
Fragments arranged by size:
2144 953 667 603 539 353 335 167
151 81 75
BmtI G_CTAG'C
Cuts at: 701 701
Size: 6068
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2757 2802 4435 2757
Size: 45 1633 4390
Fragments arranged by size:
4390 1633 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 679 1363 1498 1534 2752 461
Size: 218 684 135 36 1218 3777
Fragments arranged by size:
3777 1218 684 218 135 36
Bpu10I CC'TnA_GC
Cuts at: 181 684 2084 3052 181
Size: 503 1400 968 3197
Fragments arranged by size:
3197 1400 968 503
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3244 4123 4837 5135 3244
Size: 879 714 298 4177
Fragments arranged by size:
4177 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 470 655 1433 5772 470
Size: 185 778 4339 766
Fragments arranged by size:
4339 778 766 185
BsaAI yAC'GTr
Cuts at: 2374 3421 2374
Size: 1047 5021
BsaBI GATnn'nnATC
Cuts at: 6 1265 3582 5619 6
Size: 1259 2317 2037 455
Fragments arranged by size:
2317 2037 1259 455
BsaHI Gr'CG_yC
Cuts at: 1121 1980 3499 3747 4449 5945 1121
Size: 859 1519 248 702 1496 1244
Fragments arranged by size:
1519 1496 1244 859 702 248
BsaWI w'CCGG_w
Cuts at: 2998 3257 3778 4456 4867 5014 5288 2998
Size: 259 521 678 411 147 274 3778
Fragments arranged by size:
3778 678 521 411 274 259 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1645 1675 2302 2332 1645
Size: 30 627 30 5381
Fragments arranged by size:
5381 627 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 559 1495 1546 1768 2212 559
Size: 936 51 222 444 4415
Fragments arranged by size:
4415 936 444 222 51
BseYI C'CCAG_C
Cuts at: 812 887 1538 1672 2313 2465 2830 2858 3493
Size: 75 651 134 641 152 365 28 635
Cuts at: 3493 4251 4916 5324 812
Size: 758 665 408 1556
Fragments arranged by size:
1556 758 665 651 641 635 408 365
152 134 75 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1975 2179 2967 1975
Size: 204 788 5076
Fragments arranged by size:
5076 788 204
BsiEI CG_ry'CG
Cuts at: 104 193 230 3610 3656 4886 104
Size: 89 37 3380 46 1230 1286
Fragments arranged by size:
3380 1286 1230 89 46 37
BsiHKAI G_wGCw'C
Cuts at: 2846 3082 3860 4050 4910 2846
Size: 236 778 190 860 4004
Fragments arranged by size:
4004 860 778 236 190
Bsp1286I G_dGCh'C
Cuts at: 209 287 373 767 810 1014 1551 1631 1887
Size: 78 86 394 43 204 537 80 256
Cuts at: 1887 2031 2050 2071 2207 2294 2431 2846 2937
Size: 144 19 21 136 87 137 415 91
Cuts at: 2937 2977 3082 3693 3786 3860 4050 4112 4910
Size: 40 105 611 93 74 190 62 798
Cuts at: 4910 209
Size: 1367
Fragments arranged by size:
1367 798 611 537 415 394 256 204
190 144 137 136 105 93 91 87
86 80 78 74 62 43 40 21
19
BspHI T'CATG_A
Cuts at: 4500 4500
Size: 6068
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2024 3118 3332 3634 4015 2024
Size: 1094 214 302 381 4077
Fragments arranged by size:
4077 1094 381 302 214
BsrBI CCG'CTC
Cuts at: 50 195 1347 1518 2523 2736 4360 4414 5898
Size: 145 1152 171 1005 213 1624 54 1484
Cuts at: 5898 6063 50
Size: 165 55
Fragments arranged by size:
1624 1484 1152 1005 213 171 165 145
55 54
BsrDI GCAATG_nn'
Cuts at: 3980 3980
Size: 6068
BsrFI r'CCGG_y
Cuts at: 275 4066 4247 275
Size: 3791 181 2096
Fragments arranged by size:
3791 2096 181
BsrGI T'GTAC_A
Cuts at: 2189 2189
Size: 6068
BssSI C'ACGA_G
Cuts at: 4339 5047 4339
Size: 708 5360
BstAPI GCAn_nnn'nTGC
Cuts at: 882 3445 882
Size: 2563 3505
BstBI TT'CG_AA
Cuts at: 156 4429 156
Size: 4273 1795
BstEII G'GTnAC_C
Cuts at: 628 628
Size: 6068
BstXI CCAn_nnnn'nTGG
Cuts at: 1603 1771 1894 2230 1603
Size: 168 123 336 5441
Fragments arranged by size:
5441 336 168 123
BstYI r'GATC_y
Cuts at: 1 378 590 611 776 1163 1196 3093 3560
Size: 377 212 21 165 387 33 1897 467
Cuts at: 3560 3583 3918 4164 4470 4482 4568 5620 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1897 1052 467 449 387 377 335 306
246 212 165 86 33 23 21 12
BstZ17I GTA'TAC
Cuts at: 1724 1724
Size: 6068
Bsu36I CC'TnA_GG
Cuts at: 596 596
Size: 6068
BtgI C'CryG_G
Cuts at: 107 267 306 374 1190 1751 1830 2168 2294
Size: 160 39 68 816 561 79 338 126
Cuts at: 2294 2864 107
Size: 570 3311
Fragments arranged by size:
3311 816 570 561 338 160 126 79
68 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4018 4199 4362 4018
Size: 181 163 5724
Fragments arranged by size:
5724 181 163
BtsI GCAGTG_nn'
Cuts at: 1748 2150 2216 2329 2675 2948 3311 1748
Size: 402 66 113 346 273 363 4505
Fragments arranged by size:
4505 402 363 346 273 113 66
DraI TTT'AAA
Cuts at: 3067 3067
Size: 6068
DraIII CAC_nnn'GTG
Cuts at: 1102 1435 1102
Size: 333 5735
DrdI GACnn_nn'nnGTC
Cuts at: 3774 5118 3774
Size: 1344 4724
EaeI y'GGCC_r
Cuts at: 190 227 270 413 2006 2444 2513 2642 3653
Size: 37 43 143 1593 438 69 129 1011
Cuts at: 3653 3827 4218 4245 5590 190
Size: 174 391 27 1345 668
Fragments arranged by size:
1593 1345 1011 668 438 391 174 143
129 69 43 37 27
EagI C'GGCC_G
Cuts at: 190 227 3653 190
Size: 37 3426 2605
Fragments arranged by size:
3426 2605 37
EarI CTCTTCn'nnn_
Cuts at: 4091 4301 4091
Size: 210 5858
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1371 2566 4276 5018 5164 5878 1371
Size: 1195 1710 742 146 714 1561
Fragments arranged by size:
1710 1561 1195 742 714 146
EcoICRI GAG'CTC
Cuts at: 3080 3080
Size: 6068
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 679 1363 1376 1430 1498 1534 1808 2752
Size: 218 684 13 54 68 36 274 944
Cuts at: 2752 3893 4325 4678 5340 461
Size: 1141 432 353 662 1189
Fragments arranged by size:
1189 1141 944 684 662 432 353 274
218 68 54 36 13
EcoNI CCTnn'n_nnAGG
Cuts at: 1912 2497 1912
Size: 585 5483
EcoO109I rG'GnC_Cy
Cuts at: 177 764 1141 1379 1387 2047 2226 2391 2749
Size: 587 377 238 8 660 179 165 358
Cuts at: 2749 2826 3207 177
Size: 77 381 3038
Fragments arranged by size:
3038 660 587 381 377 358 238 179
165 77 8
EcoRI G'AATT_C
Cuts at: 3072 3072
Size: 6068
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 378 4087 378
Size: 3709 2359
FspI TGC'GCA
Cuts at: 3849 3849
Size: 6068
HaeII r_GCGC'y
Cuts at: 1620 1770 2215 3502 3750 4980 5751 1620
Size: 150 445 1287 248 1230 771 1937
Fragments arranged by size:
1937 1287 1230 771 445 248 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1125 1155 1157 1187 1350 1382 1889 1921 2302
Size: 30 2 30 163 32 507 32 381
Cuts at: 2302 2334 2755 2787 3910 3940 3942 3972 1125
Size: 32 421 32 1123 30 2 30 3221
Fragments arranged by size:
3221 1123 507 421 381 163 32 32
32 32 30 30 30 30 2 2
HincII GTy'rAC
Cuts at: 3107 3107
Size: 6068
KasI G'GCGC_C
Cuts at: 3498 3746 3498
Size: 248 5820
KpnI G_GTAC'C
Cuts at: 1237 3088 1237
Size: 1851 4217
MfeI C'AATT_G
Cuts at: 513 513
Size: 6068
MluI A'CGCG_T
Cuts at: 5501 5501
Size: 6068
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 429 1141 4827 5011 429
Size: 712 3686 184 1486
Fragments arranged by size:
3686 1486 712 184
MscI TGG'CCA
Cuts at: 272 415 2644 3829 272
Size: 143 2229 1185 2511
Fragments arranged by size:
2511 2229 1185 143
MslI CAynn'nnrTG
Cuts at: 907 1967 2839 4057 4184 907
Size: 1060 872 1218 127 2791
Fragments arranged by size:
2791 1218 1060 872 127
MspA1I CmG'CkG
Cuts at: 127 308 816 891 981 1149 1507 1676 1959
Size: 181 508 75 90 168 358 169 283
Cuts at: 1959 2091 2628 2664 2814 3179 3493 3853 4637
Size: 132 537 36 150 365 314 360 784
Cuts at: 4637 4882 127
Size: 245 1313
Fragments arranged by size:
1313 784 537 508 365 360 358 314
283 245 181 169 168 150 132 90
75 36
NaeI GCC'GGC
Cuts at: 4249 4249
Size: 6068
NarI GG'CG_CC
Cuts at: 3499 3747 3499
Size: 248 5820
NcoI C'CATG_G
Cuts at: 107 267 1190 2864 107
Size: 160 923 1674 3311
Fragments arranged by size:
3311 1674 923 160
NgoMIV G'CCGG_C
Cuts at: 4247 4247
Size: 6068
NheI G'CTAG_C
Cuts at: 697 697
Size: 6068
NruI TCG'CGA
Cuts at: 2979 2979
Size: 6068
NsiI A_TGCA'T
Cuts at: 2307 2307
Size: 6068
NspI r_CATG'y
Cuts at: 898 1966 3121 3621 5224 898
Size: 1068 1155 500 1603 1742
Fragments arranged by size:
1742 1603 1155 1068 500
PasI CC'CwG_GG
Cuts at: 1129 1383 2043 2898 1129
Size: 254 660 855 4299
Fragments arranged by size:
4299 855 660 254
PciI A'CATG_T
Cuts at: 5220 5220
Size: 6068
PflMI CCAn_nnn'nTGG
Cuts at: 279 2307 2493 2865 279
Size: 2028 186 372 3482
Fragments arranged by size:
3482 2028 372 186
PmeI GTTT'AAAC
Cuts at: 3067 3067
Size: 6068
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4514 4546 4514
Size: 32 6036
PpuMI rG'GwC_Cy
Cuts at: 1141 1379 2226 2391 1141
Size: 238 847 165 4818
Fragments arranged by size:
4818 847 238 165
PshAI GACnn'nnGTC
Cuts at: 580 580
Size: 6068
PspOMI G'GGCC_C
Cuts at: 763 1883 2046 763
Size: 1120 163 4785
Fragments arranged by size:
4785 1120 163
PstI C_TGCA'G
Cuts at: 132 216 878 1009 1616 2336 2663 3115 3800
Size: 84 662 131 607 720 327 452 685
Cuts at: 3800 132
Size: 2400
Fragments arranged by size:
2400 720 685 662 607 452 327 131
84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6068
PvuII CAG'CTG
Cuts at: 127 816 1959 2091 2814 3493 3853 127
Size: 689 1143 132 723 679 360 2342
Fragments arranged by size:
2342 1143 723 689 679 360 132
RsrII CG'GwC_CG
Cuts at: 4263 4263
Size: 6068
SacI G_AGCT'C
Cuts at: 3082 3082
Size: 6068
SacII CC_GC'GG
Cuts at: 309 309
Size: 6068
SalI G'TCGA_C
Cuts at: 3105 3105
Size: 6068
SapI GCTCTTCn'nnn_
Cuts at: 4091 4301 4091
Size: 210 5858
SbfI CC_TGCA'GG
Cuts at: 3115 3115
Size: 6068
ScaI AGT'ACT
Cuts at: 5585 5585
Size: 6068
SexAI A'CCwGG_T
Cuts at: 833 1304 1838 1949 2393 833
Size: 471 534 111 444 4508
Fragments arranged by size:
4508 534 471 444 111
SfcI C'TryA_G
Cuts at: 33 128 212 874 1005 1238 1612 2332 2384
Size: 95 84 662 131 233 374 720 52
Cuts at: 2384 2659 3111 3796 4764 4955 33
Size: 275 452 685 968 191 1146
Fragments arranged by size:
1146 968 720 685 662 452 374 275
233 191 131 95 84 52
SfiI GGCCn_nnn'nGGCC
Cuts at: 235 2470 235
Size: 2235 3833
SfoI GGC'GCC
Cuts at: 3500 3748 3500
Size: 248 5820
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6068
SmaI CCC'GGG
Cuts at: 1882 3090 1882
Size: 1208 4860
SmlI C'TyrA_G
Cuts at: 3223 4138 4852 5114 3223
Size: 915 714 262 4177
Fragments arranged by size:
4177 915 714 262
SnaBI TAC'GTA
Cuts at: 2374 2374
Size: 6068
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6068
SphI G_CATG'C
Cuts at: 3121 3621 3121
Size: 500 5568
SrfI GCCC'GGGC
Cuts at: 1882 1882
Size: 6068
SspI AAT'ATT
Cuts at: 535 535
Size: 6068
StuI AGG'CCT
Cuts at: 223 1975 2929 223
Size: 1752 954 3362
Fragments arranged by size:
3362 1752 954
StyI C'CwwG_G
Cuts at: 107 218 267 635 1190 1221 1454 1620 2423
Size: 111 49 368 555 31 233 166 803
Cuts at: 2423 2570 2864 2991 3202 3556 107
Size: 147 294 127 211 354 2619
Fragments arranged by size:
2619 803 555 368 354 294 233 211
166 147 127 111 49 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2026 2125 3651 3784 4243 4452 2026
Size: 99 1526 133 459 209 3642
Fragments arranged by size:
3642 1526 459 209 133 99
TatI w'GTAC_w
Cuts at: 769 1652 2189 5583 769
Size: 883 537 3394 1254
Fragments arranged by size:
3394 1254 883 537
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 363 395 1202 1234 5585 5617 363
Size: 32 807 32 4351 32 814
Fragments arranged by size:
4351 814 807 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2767 3865 2767
Size: 1098 4970
XbaI T'CTAG_A
Cuts at: 3099 3099
Size: 6068
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 642 3563 642
Size: 2921 3147
XmaI C'CCGG_G
Cuts at: 1880 3088 1880
Size: 1208 4860
XmnI GAAnn'nnTTC
Cuts at: 349 349
Size: 6068
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NruI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AscI BaeI BcgI BsiWI
BsmI BsmBI BspEI BssHII ClaI CspCI EcoRV FseI
FspAI HindIII HpaI NdeI NotI PacI PfoI PmlI
PsiI PspXI PsrI SanDI SgrAI SwaI XhoI ZraI