Restriction map for
pF1KA1273
(Circular) (Six-base) MAPSORT of: pF1KA1273.seq Check: 6068 from: 1 to: 4919
pF1KA1273 4919 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AatII G_ACGT'C
Cuts at: 835 835
Size: 4919
AccI GT'mk_AC
Cuts at: 752 897 1957 752
Size: 145 1060 3714
Fragments arranged by size:
3714 1060 145
Acc65I G'GTAC_C
Cuts at: 1935 1935
Size: 4919
AclI AA'CG_TT
Cuts at: 4759 4759
Size: 4919
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 628 1501 1705 2744 3176 3923 4191 424
Size: 204 873 204 1039 432 747 268 1152
Fragments arranged by size:
1152 1039 873 747 432 268 204 204
AfeI AGC'GCT
Cuts at: 388 388
Size: 4919
AflII C'TTAA_G
Cuts at: 1450 1450
Size: 4919
AflIII A'CryG_T
Cuts at: 2271 3375 4352 2271
Size: 1104 977 2838
Fragments arranged by size:
2838 1104 977
AgeI A'CCGG_T
Cuts at: 3307 3307
Size: 4919
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 862 862
Size: 4919
AlwNI CAG_nnn'CTG
Cuts at: 2224 3791 2224
Size: 1567 3352
ApaI G_GGCC'C
Cuts at: 360 360
Size: 4919
ApaLI G'TGCA_C
Cuts at: 3689 3689
Size: 4919
ApoI r'AATT_y
Cuts at: 432 1306 1923 4272 4528 432
Size: 874 617 2349 256 823
Fragments arranged by size:
2349 874 823 617 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4919
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4919
AvaI C'yCGr_G
Cuts at: 548 1939 548
Size: 1391 3528
BamHI G'GATC_C
Cuts at: 1944 1944
Size: 4919
BanI G'GyrC_C
Cuts at: 658 863 1033 1475 1901 1935 2349 2597 2632
Size: 205 170 442 426 34 414 248 35
Cuts at: 2632 658
Size: 2945
Fragments arranged by size:
2945 442 426 414 248 205 170 35
34
BanII G_rGCy'C
Cuts at: 360 1933 2963 360
Size: 1573 1030 2316
Fragments arranged by size:
2316 1573 1030
BbeI G_GCGC'C
Cuts at: 1479 2353 2601 1479
Size: 874 248 3797
Fragments arranged by size:
3797 874 248
BbsI GAAGACnn'nnnn_
Cuts at: 325 1048 325
Size: 723 4196
BbvCI CC'TCA_GC
Cuts at: 305 305
Size: 4919
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4885
BciVI GTATCCnnnnn_n'
Cuts at: 2817 3578 4919 2817
Size: 761 1341 2817
Fragments arranged by size:
2817 1341 761
BclI T'GATC_A
Cuts at: 2439 2439
Size: 4919
BglI GCCn_nnn'nGGC
Cuts at: 721 1181 1793 721
Size: 460 612 3847
Fragments arranged by size:
3847 612 460
BglII A'GATC_T
Cuts at: 2434 4471 2434
Size: 2037 2882
BlpI GC'TnA_GC
Cuts at: 1562 2031 1562
Size: 469 4450
Bme1580I G_kGCm'C
Cuts at: 153 360 1843 1904 2544 2637 3693 153
Size: 207 1483 61 640 93 1056 1379
Fragments arranged by size:
1483 1379 1056 640 207 93 61
BmgBI CAC'GTC
Cuts at: 909 1110 1374 909
Size: 201 264 4454
Fragments arranged by size:
4454 264 201
BmrI ACTGGGnnnn_n'
Cuts at: 354 1170 1485 1841 2131 2212 2547 4691 354
Size: 816 315 356 290 81 335 2144 582
Fragments arranged by size:
2144 816 582 356 335 315 290 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3286 3286
Size: 4919
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 934 871
Size: 63 4856
Bpu10I CC'TnA_GC
Cuts at: 215 305 325 1337 215
Size: 90 20 1012 3797
Fragments arranged by size:
3797 1012 90 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 2095 2974 3466 3764 262
Size: 1833 879 492 298 1417
Fragments arranged by size:
1833 1417 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 328 532 1735 4623 328
Size: 204 1203 2888 624
Fragments arranged by size:
2888 1203 624 204
BsaAI yAC'GTr
Cuts at: 2272 2272
Size: 4919
BsaBI GATnn'nnATC
Cuts at: 6 2433 4470 6
Size: 2427 2037 455
Fragments arranged by size:
2427 2037 455
BsaHI Gr'CG_yC
Cuts at: 613 832 1211 1476 1634 2350 2598 3300 4796
Size: 219 379 265 158 716 248 702 1496
Cuts at: 4796 613
Size: 736
Fragments arranged by size:
1496 736 716 702 379 265 248 219
158
BsaWI w'CCGG_w
Cuts at: 1726 2108 2629 3307 3581 3728 4139 1726
Size: 382 521 678 274 147 411 2506
Fragments arranged by size:
2506 678 521 411 382 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 181 211 1528 1558 181
Size: 30 1317 30 3542
Fragments arranged by size:
3542 1317 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 322 327 346 442 463 880 1252 1420 322
Size: 5 19 96 21 417 372 168 3821
Fragments arranged by size:
3821 417 372 168 96 21 19 5
BseYI C'CCAG_C
Cuts at: 724 804 934 2344 3102 3679 4175 724
Size: 80 130 1410 758 577 496 1468
Fragments arranged by size:
1468 1410 758 577 496 130 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 199 478 1054 1785 118
Size: 81 279 576 731 3252
Fragments arranged by size:
3252 731 576 279 81
BsiEI CG_ry'CG
Cuts at: 104 1881 1885 2461 2507 3715 104
Size: 1777 4 576 46 1208 1308
Fragments arranged by size:
1777 1308 1208 576 46 4
BsiHKAI G_wGCw'C
Cuts at: 246 623 1516 1718 1933 2711 2901 3693 246
Size: 377 893 202 215 778 190 792 1472
Fragments arranged by size:
1472 893 792 778 377 215 202 190
BsmI GAATG_Cn'
Cuts at: 582 771 582
Size: 189 4730
BsmBI CGTCTCn'nnnn_
Cuts at: 823 823
Size: 4919
Bsp1286I G_dGCh'C
Cuts at: 153 246 360 623 1516 1718 1843 1904 1933
Size: 93 114 263 893 202 125 61 29
Cuts at: 1933 2544 2637 2711 2901 2963 3693 153
Size: 611 93 74 190 62 730 1379
Fragments arranged by size:
1379 893 730 611 263 202 190 125
114 93 93 74 62 61 29
BspEI T'CCGG_A
Cuts at: 1726 4139 1726
Size: 2413 2506
BspHI T'CATG_A
Cuts at: 1094 4095 1094
Size: 3001 1918
BspMI ACCTGCnnnn'nnnn_
Cuts at: 137 1400 1969 2183 2485 2866 137
Size: 1263 569 214 302 381 2190
Fragments arranged by size:
2190 1263 569 381 302 214
BsrBI CCG'CTC
Cuts at: 50 192 495 570 1410 3211 3265 4749 4914
Size: 142 303 75 840 1801 54 1484 165
Cuts at: 4914 50
Size: 55
Fragments arranged by size:
1801 1484 840 303 165 142 75 55
54
BsrDI GCAATG_nn'
Cuts at: 2831 2831
Size: 4919
BsrFI r'CCGG_y
Cuts at: 881 1175 1455 1792 2917 3098 3307 881
Size: 294 280 337 1125 181 209 2493
Fragments arranged by size:
2493 1125 337 294 280 209 181
BsrGI T'GTAC_A
Cuts at: 1001 1001
Size: 4919
BssHII G'CGCG_C
Cuts at: 553 555 553
Size: 2 4917
BssSI C'ACGA_G
Cuts at: 3190 3548 3190
Size: 358 4561
BstAPI GCAn_nnn'nTGC
Cuts at: 1014 2296 1014
Size: 1282 3637
BstBI TT'CG_AA
Cuts at: 3280 3280
Size: 4919
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4919
BstYI r'GATC_y
Cuts at: 1 290 599 1944 2411 2434 2769 3015 4027
Size: 289 309 1345 467 23 335 246 1012
Cuts at: 4027 4113 4125 4471 1
Size: 86 12 346 449
Fragments arranged by size:
1345 1012 467 449 346 335 309 289
246 86 23 12
BtgI C'CryG_G
Cuts at: 266 488 716 1118 1586 1766 266
Size: 222 228 402 468 180 3419
Fragments arranged by size:
3419 468 402 228 222 180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 945 2869 3050 3213 945
Size: 1924 181 163 2651
Fragments arranged by size:
2651 1924 181 163
BtsI GCAGTG_nn'
Cuts at: 210 2162 210
Size: 1952 2967
DraI TTT'AAA
Cuts at: 1918 1918
Size: 4919
DrdI GACnn_nn'nnGTC
Cuts at: 1378 2625 3483 1378
Size: 1247 858 2814
Fragments arranged by size:
2814 1247 858
EaeI y'GGCC_r
Cuts at: 783 1322 1457 1568 1763 1882 2504 2678 3069
Size: 539 135 111 195 119 622 174 391
Cuts at: 3069 3096 4441 783
Size: 27 1345 1261
Fragments arranged by size:
1345 1261 622 539 391 195 174 135
119 111 27
EagI C'GGCC_G
Cuts at: 1882 2504 1882
Size: 622 4297
EarI CTCTTCn'nnn_
Cuts at: 1198 2942 3152 1198
Size: 1744 210 2965
Fragments arranged by size:
2965 1744 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 592 1705 3127 3437 3583 4729 592
Size: 1113 1422 310 146 1146 782
Fragments arranged by size:
1422 1146 1113 782 310 146
EcoICRI GAG'CTC
Cuts at: 1931 1931
Size: 4919
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 628 871 934 1501 1705 2744 3176 3923
Size: 204 243 63 567 204 1039 432 747
Cuts at: 3923 4191 424
Size: 268 1152
Fragments arranged by size:
1152 1039 747 567 432 268 243 204
204 63
EcoNI CCTnn'n_nnAGG
Cuts at: 328 1618 328
Size: 1290 3629
EcoO109I rG'GnC_Cy
Cuts at: 356 996 1251 1611 1696 1846 2058 356
Size: 640 255 360 85 150 212 3217
Fragments arranged by size:
3217 640 360 255 212 150 85
EcoRI G'AATT_C
Cuts at: 1923 1923
Size: 4919
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2938 2938
Size: 4919
FspI TGC'GCA
Cuts at: 2700 2700
Size: 4919
HaeII r_GCGC'y
Cuts at: 390 859 1419 1479 2353 2601 3623 4602 390
Size: 469 560 60 874 248 1022 979 707
Fragments arranged by size:
1022 979 874 707 560 469 248 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 181 213 1528 1560 2761 2791 2793 2823 181
Size: 32 1315 32 1201 30 2 30 2277
Fragments arranged by size:
2277 1315 1201 32 32 30 30 2
HincII GTy'rAC
Cuts at: 898 1958 898
Size: 1060 3859
KasI G'GCGC_C
Cuts at: 1475 2349 2597 1475
Size: 874 248 3797
Fragments arranged by size:
3797 874 248
KpnI G_GTAC'C
Cuts at: 1939 1939
Size: 4919
MluI A'CGCG_T
Cuts at: 4352 4352
Size: 4919
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3590 3774 3590
Size: 184 4735
MscI TGG'CCA
Cuts at: 1324 1765 2680 1324
Size: 441 915 3563
Fragments arranged by size:
3563 915 441
MslI CAynn'nnrTG
Cuts at: 518 844 1372 2908 3035 518
Size: 326 528 1536 127 2402
Fragments arranged by size:
2402 1536 528 326 127
MspA1I CmG'CkG
Cuts at: 114 229 1566 2030 2344 2704 3717 3962 114
Size: 115 1337 464 314 360 1013 245 1071
Fragments arranged by size:
1337 1071 1013 464 360 314 245 115
NaeI GCC'GGC
Cuts at: 883 1177 1457 1794 3100 883
Size: 294 280 337 1306 2702
Fragments arranged by size:
2702 1306 337 294 280
NarI GG'CG_CC
Cuts at: 1476 2350 2598 1476
Size: 874 248 3797
Fragments arranged by size:
3797 874 248
NcoI C'CATG_G
Cuts at: 1118 1118
Size: 4919
NdeI CA'TA_TG
Cuts at: 1593 1593
Size: 4919
NgoMIV G'CCGG_C
Cuts at: 881 1175 1455 1792 3098 881
Size: 294 280 337 1306 2702
Fragments arranged by size:
2702 1306 337 294 280
NspI r_CATG'y
Cuts at: 1545 1972 2472 3379 1545
Size: 427 500 907 3085
Fragments arranged by size:
3085 907 500 427
PasI CC'CwG_GG
Cuts at: 1843 1897 1843
Size: 54 4865
PciI A'CATG_T
Cuts at: 3375 3375
Size: 4919
PfoI T'CCnGG_A
Cuts at: 1819 1819
Size: 4919
PmeI GTTT'AAAC
Cuts at: 1918 1918
Size: 4919
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 4058
Size: 32 4887
PpuMI rG'GwC_Cy
Cuts at: 1251 1611 1251
Size: 360 4559
PshAI GACnn'nnGTC
Cuts at: 775 775
Size: 4919
PspOMI G'GGCC_C
Cuts at: 356 356
Size: 4919
PstI C_TGCA'G
Cuts at: 251 1966 2651 251
Size: 1715 685 2519
Fragments arranged by size:
2519 1715 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4919
PvuII CAG'CTG
Cuts at: 114 229 1566 2344 2704 114
Size: 115 1337 778 360 2329
Fragments arranged by size:
2329 1337 778 360 115
RsrII CG'GwC_CG
Cuts at: 3114 3114
Size: 4919
SacI G_AGCT'C
Cuts at: 1933 1933
Size: 4919
SalI G'TCGA_C
Cuts at: 896 1956 896
Size: 1060 3859
SapI GCTCTTCn'nnn_
Cuts at: 1198 2942 3152 1198
Size: 1744 210 2965
Fragments arranged by size:
2965 1744 210
SbfI CC_TGCA'GG
Cuts at: 1966 1966
Size: 4919
ScaI AGT'ACT
Cuts at: 4436 4436
Size: 4919
SfcI C'TryA_G
Cuts at: 33 247 1962 2647 3640 3831 33
Size: 214 1715 685 993 191 1121
Fragments arranged by size:
1715 1121 993 685 214 191
SfoI GGC'GCC
Cuts at: 1477 2351 2599 1477
Size: 874 248 3797
Fragments arranged by size:
3797 874 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4919
SmaI CCC'GGG
Cuts at: 550 1941 550
Size: 1391 3528
SmlI C'TyrA_G
Cuts at: 277 1450 2074 2989 3481 3743 277
Size: 1173 624 915 492 262 1453
Fragments arranged by size:
1453 1173 915 624 492 262
SpeI A'CTAG_T
Cuts at: 67 141 67
Size: 74 4845
SphI G_CATG'C
Cuts at: 1972 2472 1972
Size: 500 4419
SrfI GCCC'GGGC
Cuts at: 550 550
Size: 4919
StyI C'CwwG_G
Cuts at: 560 1118 1601 1734 2053 2407 560
Size: 558 483 133 319 354 3072
Fragments arranged by size:
3072 558 483 354 319 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 556 2502 2635 3094 3303 556
Size: 1946 133 459 209 2172
Fragments arranged by size:
2172 1946 459 209 133
TatI w'GTAC_w
Cuts at: 1001 4434 1001
Size: 3433 1486
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1708 1740 1854 1886 4436 4468 1708
Size: 32 114 32 2550 32 2159
Fragments arranged by size:
2550 2159 114 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1609 2716 1609
Size: 1107 3812
XbaI T'CTAG_A
Cuts at: 1950 1950
Size: 4919
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2414 2414
Size: 4919
XmaI C'CCGG_G
Cuts at: 548 1939 548
Size: 1391 3528
XmnI GAAnn'nnTTC
Cuts at: 1273 1273
Size: 4919
ZraI GAC'GTC
Cuts at: 833 833
Size: 4919
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AhdI AleI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PflMI PmlI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SspI StuI
SwaI XhoI