Restriction map for
pF1KA1275
(Circular) (Six-base) MAPSORT of: pF1KA1275.seq Check: 3615 from: 1 to: 6644
pF1KA1275 6644 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1981 2616 1981
Size: 635 6009
AatII G_ACGT'C
Cuts at: 2512 2512
Size: 6644
AccI GT'mk_AC
Cuts at: 647 1802 3145 3682 647
Size: 1155 1343 537 3609
Fragments arranged by size:
3609 1343 1155 537
Acc65I G'GTAC_C
Cuts at: 3660 3660
Size: 6644
AclI AA'CG_TT
Cuts at: 2984 6484 2984
Size: 3500 3144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 2044 2924 3335 4469 4901 5648 5916 430
Size: 1614 880 411 1134 432 747 268 1158
Fragments arranged by size:
1614 1158 1134 880 747 432 411 268
AfeI AGC'GCT
Cuts at: 2449 2449
Size: 6644
AflIII A'CryG_T
Cuts at: 3996 5100 6077 3996
Size: 1104 977 4563
Fragments arranged by size:
4563 1104 977
AgeI A'CCGG_T
Cuts at: 3504 5032 3504
Size: 1528 5116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 512 2186 512
Size: 1674 4970
AlwNI CAG_nnn'CTG
Cuts at: 268 930 2835 3949 5516 268
Size: 662 1905 1114 1567 1396
Fragments arranged by size:
1905 1567 1396 1114 662
ApaI G_GGCC'C
Cuts at: 2771 2771
Size: 6644
ApaLI G'TGCA_C
Cuts at: 1452 5414 1452
Size: 3962 2682
ApoI r'AATT_y
Cuts at: 1701 2104 2778 2908 3305 3648 5997 6253 1701
Size: 403 674 130 397 343 2349 256 2092
Fragments arranged by size:
2349 2092 674 403 397 343 256 130
AseI AT'TA_AT
Cuts at: 20 2308 20
Size: 2288 4356
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6644
AvaI C'yCGr_G
Cuts at: 3524 3664 3524
Size: 140 6504
BamHI G'GATC_C
Cuts at: 3669 3669
Size: 6644
BanI G'GyrC_C
Cuts at: 2792 3660 4074 4322 4357 2792
Size: 868 414 248 35 5079
Fragments arranged by size:
5079 868 414 248 35
BanII G_rGCy'C
Cuts at: 1370 1970 2463 2696 2771 3658 4688 1370
Size: 600 493 233 75 887 1030 3326
Fragments arranged by size:
3326 1030 887 600 493 233 75
BbeI G_GCGC'C
Cuts at: 4078 4326 4078
Size: 248 6396
BbsI GAAGACnn'nnnn_
Cuts at: 347 418 986 2077 3082 347
Size: 71 568 1091 1005 3909
Fragments arranged by size:
3909 1091 1005 568 71
BbvCI CC'TCA_GC
Cuts at: 931 931
Size: 6644
BciVI GTATCCnnnnn_n'
Cuts at: 2684 4542 5303 6644 2684
Size: 1858 761 1341 2684
Fragments arranged by size:
2684 1858 1341 761
BclI T'GATC_A
Cuts at: 2088 4164 2088
Size: 2076 4568
BglII A'GATC_T
Cuts at: 112 2653 4159 6196 112
Size: 2541 1506 2037 560
Fragments arranged by size:
2541 2037 1506 560
BlpI GC'TnA_GC
Cuts at: 3756 3756
Size: 6644
Bme1580I G_kGCm'C
Cuts at: 1456 2771 2795 4269 4362 5418 1456
Size: 1315 24 1474 93 1056 2682
Fragments arranged by size:
2682 1474 1315 1056 93 24
BmrI ACTGGGnnnn_n'
Cuts at: 1308 2465 3040 3528 3856 3937 4272 6416 1308
Size: 1157 575 488 328 81 335 2144 1536
Fragments arranged by size:
2144 1536 1157 575 488 335 328 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1055 5011 1055
Size: 3956 2688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 346 460 739 1495 2323 2537 2663 3065
Size: 32 114 279 756 828 214 126 402
Cuts at: 3065 3236 3618 314
Size: 171 382 3340
Fragments arranged by size:
3340 828 756 402 382 279 214 171
126 114 32
Bpu10I CC'TnA_GC
Cuts at: 931 931
Size: 6644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 982 1025 1090 3820 4699 5191 5489 589
Size: 393 43 65 2730 879 492 298 1744
Fragments arranged by size:
2730 1744 879 492 393 298 65 43
BsaI GGTCTCn'nnnn_
Cuts at: 3329 6348 3329
Size: 3019 3625
BsaAI yAC'GTr
Cuts at: 3997 3997
Size: 6644
BsaBI GATnn'nnATC
Cuts at: 6 4158 6195 6
Size: 4152 2037 455
Fragments arranged by size:
4152 2037 455
BsaHI Gr'CG_yC
Cuts at: 2509 4075 4323 5025 6521 2509
Size: 1566 248 702 1496 2632
Fragments arranged by size:
2632 1566 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3504 3833 4354 5032 5306 5453 5864 3504
Size: 329 521 678 274 147 411 4284
Fragments arranged by size:
4284 678 521 411 329 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 518 548 2731 2761 2792 2822 518
Size: 30 2183 30 31 30 4340
Fragments arranged by size:
4340 2183 31 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1150 1306 2167 3447 1150
Size: 156 861 1280 4347
Fragments arranged by size:
4347 1280 861 156
BseYI C'CCAG_C
Cuts at: 1457 3563 4069 4827 5404 5900 1457
Size: 2106 506 758 577 496 2201
Fragments arranged by size:
2201 2106 758 577 506 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 472
Size: 6644
BsiEI CG_ry'CG
Cuts at: 104 4186 4232 5440 104
Size: 4082 46 1208 1308
Fragments arranged by size:
4082 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1370 1456 1970 2463 2696 3490 3658 4436 4626
Size: 86 514 493 233 794 168 778 190
Cuts at: 4626 5418 1370
Size: 792 2596
Fragments arranged by size:
2596 794 792 778 514 493 233 190
168 86
BsmI GAATG_Cn'
Cuts at: 3584 3584
Size: 6644
BsmBI CGTCTCn'nnnn_
Cuts at: 2038 2038
Size: 6644
Bsp1286I G_dGCh'C
Cuts at: 1370 1456 1970 2463 2696 2771 2795 3490 3658
Size: 86 514 493 233 75 24 695 168
Cuts at: 3658 4269 4362 4436 4626 4688 5418 1370
Size: 611 93 74 190 62 730 2596
Fragments arranged by size:
2596 730 695 611 514 493 233 190
168 93 86 75 74 62 24
BspEI T'CCGG_A
Cuts at: 5864 5864
Size: 6644
BspHI T'CATG_A
Cuts at: 5820 5820
Size: 6644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 722 1511 1981 2616 3694 3908 4210 4591 722
Size: 789 470 635 1078 214 302 381 2775
Fragments arranged by size:
2775 1078 789 635 470 381 302 214
BsrBI CCG'CTC
Cuts at: 50 729 4936 4990 6474 6639 50
Size: 679 4207 54 1484 165 55
Fragments arranged by size:
4207 1484 679 165 55 54
BsrDI GCAATG_nn'
Cuts at: 189 4556 189
Size: 4367 2277
BsrFI r'CCGG_y
Cuts at: 593 3421 3504 4642 4823 5032 593
Size: 2828 83 1138 181 209 2205
Fragments arranged by size:
2828 2205 1138 209 181 83
BssSI C'ACGA_G
Cuts at: 2860 4915 5273 2860
Size: 2055 358 4231
Fragments arranged by size:
4231 2055 358
BstAPI GCAn_nnn'nTGC
Cuts at: 615 4021 615
Size: 3406 3238
BstBI TT'CG_AA
Cuts at: 1257 5005 1257
Size: 3748 2896
BstXI CCAn_nnnn'nTGG
Cuts at: 717 2433 717
Size: 1716 4928
BstYI r'GATC_y
Cuts at: 1 112 915 2653 3669 4136 4159 4494 4740
Size: 111 803 1738 1016 467 23 335 246
Cuts at: 4740 5752 5838 5850 6196 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1738 1016 1012 803 467 449 346 335
246 111 86 23 12
BstZ17I GTA'TAC
Cuts at: 648 1803 648
Size: 1155 5489
Bsu36I CC'TnA_GG
Cuts at: 261 431 2940 261
Size: 170 2509 3965
Fragments arranged by size:
3965 2509 170
BtgI C'CryG_G
Cuts at: 2645 2719 3200 2645
Size: 74 481 6089
Fragments arranged by size:
6089 481 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4594 4775 4938 4594
Size: 181 163 6300
Fragments arranged by size:
6300 181 163
BtsI GCAGTG_nn'
Cuts at: 1887 2870 3887 1887
Size: 983 1017 4644
Fragments arranged by size:
4644 1017 983
DraI TTT'AAA
Cuts at: 1469 1772 3643 1469
Size: 303 1871 4470
Fragments arranged by size:
4470 1871 303
DrdI GACnn_nn'nnGTC
Cuts at: 4350 5208 4350
Size: 858 5786
EaeI y'GGCC_r
Cuts at: 620 4229 4403 4794 4821 6166 620
Size: 3609 174 391 27 1345 1098
Fragments arranged by size:
3609 1345 1098 391 174 27
EagI C'GGCC_G
Cuts at: 4229 4229
Size: 6644
EarI CTCTTCn'nnn_
Cuts at: 217 938 1046 1139 1390 1667 1894 3087 4667
Size: 721 108 93 251 277 227 1193 1580
Cuts at: 4667 4877 217
Size: 210 1984
Fragments arranged by size:
1984 1580 1193 721 277 251 227 210
108 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4852 5162 5308 6454 4852
Size: 310 146 1146 5042
Fragments arranged by size:
5042 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1368 1968 2461 2694 3656 1368
Size: 600 493 233 962 4356
Fragments arranged by size:
4356 962 600 493 233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 346 430 460 739 1495 2044 2323 2537
Size: 32 84 30 279 756 549 279 214
Cuts at: 2537 2663 2924 3065 3236 3335 3618 4469 4901
Size: 126 261 141 171 99 283 851 432
Cuts at: 4901 5648 5916 314
Size: 747 268 1042
Fragments arranged by size:
1042 851 756 747 549 432 283 279
279 268 261 214 171 141 126 99
84 32 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2939 2939
Size: 6644
EcoO109I rG'GnC_Cy
Cuts at: 483 2767 2768 3783 483
Size: 2284 1 1015 3344
Fragments arranged by size:
3344 2284 1015 1
EcoRI G'AATT_C
Cuts at: 3648 3648
Size: 6644
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1615 2148 2344 4663 1615
Size: 533 196 2319 3596
Fragments arranged by size:
3596 2319 533 196
FspI TGC'GCA
Cuts at: 4425 4425
Size: 6644
HaeII r_GCGC'y
Cuts at: 2451 4078 4326 5348 6327 2451
Size: 1627 248 1022 979 2768
Fragments arranged by size:
2768 1627 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 518 550 2792 2824 3197 3229 3544 3576 4486
Size: 32 2242 32 373 32 315 32 910
Cuts at: 4486 4516 4518 4548 518
Size: 30 2 30 2614
Fragments arranged by size:
2614 2242 910 373 315 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1887 2513 3683 1887
Size: 626 1170 4848
Fragments arranged by size:
4848 1170 626
KasI G'GCGC_C
Cuts at: 4074 4322 4074
Size: 248 6396
KpnI G_GTAC'C
Cuts at: 3664 3664
Size: 6644
MfeI C'AATT_G
Cuts at: 2041 2041
Size: 6644
MluI A'CGCG_T
Cuts at: 6077 6077
Size: 6644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 375 2061 2222 2414 3271 3408 5315 5499 375
Size: 1686 161 192 857 137 1907 184 1520
Fragments arranged by size:
1907 1686 1520 857 192 184 161 137
MscI TGG'CCA
Cuts at: 4405 4405
Size: 6644
MslI CAynn'nnrTG
Cuts at: 494 856 3589 4633 4760 494
Size: 362 2733 1044 127 2378
Fragments arranged by size:
2733 2378 1044 362 127
MspA1I CmG'CkG
Cuts at: 612 2094 2540 2569 3163 3567 3755 4069 4429
Size: 1482 446 29 594 404 188 314 360
Cuts at: 4429 5442 5687 612
Size: 1013 245 1569
Fragments arranged by size:
1569 1482 1013 594 446 404 360 314
245 188 29
NaeI GCC'GGC
Cuts at: 4825 4825
Size: 6644
NarI GG'CG_CC
Cuts at: 4075 4323 4075
Size: 248 6396
NcoI C'CATG_G
Cuts at: 2719 3200 2719
Size: 481 6163
NgoMIV G'CCGG_C
Cuts at: 4823 4823
Size: 6644
NspI r_CATG'y
Cuts at: 408 603 3697 4197 5104 408
Size: 195 3094 500 907 1948
Fragments arranged by size:
3094 1948 907 500 195
PasI CC'CwG_GG
Cuts at: 3343 3343
Size: 6644
PciI A'CATG_T
Cuts at: 5100 5100
Size: 6644
PflMI CCAn_nnn'nTGG
Cuts at: 2043 3088 2043
Size: 1045 5599
PfoI T'CCnGG_A
Cuts at: 2591 2591
Size: 6644
PmeI GTTT'AAAC
Cuts at: 3643 3643
Size: 6644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5783 5815 5783
Size: 32 6612
PpuMI rG'GwC_Cy
Cuts at: 483 483
Size: 6644
PspOMI G'GGCC_C
Cuts at: 2767 2767
Size: 6644
PspXI vC'TCGA_Gb
Cuts at: 3524 3524
Size: 6644
PstI C_TGCA'G
Cuts at: 1166 1882 3691 4376 1166
Size: 716 1809 685 3434
Fragments arranged by size:
3434 1809 716 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6644
PvuII CAG'CTG
Cuts at: 612 2094 2569 3163 4069 4429 612
Size: 1482 475 594 906 360 2827
Fragments arranged by size:
2827 1482 906 594 475 360
RsrII CG'GwC_CG
Cuts at: 4839 4839
Size: 6644
SacI G_AGCT'C
Cuts at: 1370 1970 2463 2696 3658 1370
Size: 600 493 233 962 4356
Fragments arranged by size:
4356 962 600 493 233
SalI G'TCGA_C
Cuts at: 3681 3681
Size: 6644
SapI GCTCTTCn'nnn_
Cuts at: 4667 4877 4667
Size: 210 6434
SbfI CC_TGCA'GG
Cuts at: 3691 3691
Size: 6644
ScaI AGT'ACT
Cuts at: 6161 6161
Size: 6644
SfcI C'TryA_G
Cuts at: 33 1162 1390 1878 2451 3687 4372 5365 5556
Size: 1129 228 488 573 1236 685 993 191
Cuts at: 5556 33
Size: 1121
Fragments arranged by size:
1236 1129 1121 993 685 573 488 228
191
SfoI GGC'GCC
Cuts at: 4076 4324 4076
Size: 248 6396
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6644
SmaI CCC'GGG
Cuts at: 3666 3666
Size: 6644
SmlI C'TyrA_G
Cuts at: 568 997 1040 1105 3524 3799 4714 5206 5468
Size: 429 43 65 2419 275 915 492 262
Cuts at: 5468 568
Size: 1744
Fragments arranged by size:
2419 1744 915 492 429 275 262 65
43
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6644
SphI G_CATG'C
Cuts at: 603 3697 4197 603
Size: 3094 500 3050
Fragments arranged by size:
3094 3050 500
StyI C'CwwG_G
Cuts at: 123 2719 3200 3778 4132 123
Size: 2596 481 578 354 2635
Fragments arranged by size:
2635 2596 578 481 354
SwaI ATTT'AAAT
Cuts at: 1469 1469
Size: 6644
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1471 2810 3591 4227 4360 4819 5028 1471
Size: 1339 781 636 133 459 209 3087
Fragments arranged by size:
3087 1339 781 636 459 209 133
TatI w'GTAC_w
Cuts at: 909 2277 2355 6159 909
Size: 1368 78 3804 1394
Fragments arranged by size:
3804 1394 1368 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2916 2948 3339 3371 3570 3602 6161 6193 2916
Size: 32 391 32 199 32 2559 32 3367
Fragments arranged by size:
3367 2559 391 199 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3141 4441 3141
Size: 1300 5344
XbaI T'CTAG_A
Cuts at: 2664 3675 2664
Size: 1011 5633
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4139 4139
Size: 6644
XhoI C'TCGA_G
Cuts at: 3524 3524
Size: 6644
XmaI C'CCGG_G
Cuts at: 3664 3664
Size: 6644
XmnI GAAnn'nnTTC
Cuts at: 1181 1253 2587 2778 1181
Size: 72 1334 191 5047
Fragments arranged by size:
5047 1334 191 72
ZraI GAC'GTC
Cuts at: 2510 2510
Size: 6644
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AhdI AleI AloI AscI AvrII BaeI BcgI
BfrBI BglI BmgBI BmtI BsiWI BsrGI BssHII BstEII
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI