Restriction map for
pF1KA1282
(Circular) (Six-base) MAPSORT of: pF1KA1282.seq Check: 776 from: 1 to: 6362
pF1KA1282 6362 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 656 1832 2058 2769 656
Size: 1176 226 711 4249
Fragments arranged by size:
4249 1176 711 226
AccI GT'mk_AC
Cuts at: 236 3400 236
Size: 3164 3198
Acc65I G'GTAC_C
Cuts at: 3378 3378
Size: 6362
AclI AA'CG_TT
Cuts at: 6202 6202
Size: 6362
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 2166 4187 4619 4972 5634 1129
Size: 1037 2021 432 353 662 1857
Fragments arranged by size:
2021 1857 1037 662 432 353
AfeI AGC'GCT
Cuts at: 1049 1049
Size: 6362
AflIII A'CryG_T
Cuts at: 1085 1517 3714 5514 5795 1085
Size: 432 2197 1800 281 1652
Fragments arranged by size:
2197 1800 1652 432 281
AgeI A'CCGG_T
Cuts at: 1157 1157
Size: 6362
AleI CACnn'nnGTG
Cuts at: 958 1501 958
Size: 543 5819
AlwNI CAG_nnn'CTG
Cuts at: 896 1311 2124 2784 2919 3202 3667 5105 896
Size: 415 813 660 135 283 465 1438 2153
Fragments arranged by size:
2153 1438 813 660 465 415 283 135
ApaI G_GGCC'C
Cuts at: 532 1101 2323 2517 2714 3194 532
Size: 569 1222 194 197 480 3700
Fragments arranged by size:
3700 1222 569 480 197 194
ApaLI G'TGCA_C
Cuts at: 5200 5200
Size: 6362
ApoI r'AATT_y
Cuts at: 942 3366 5715 5971 942
Size: 2424 2349 256 1333
Fragments arranged by size:
2424 2349 1333 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6362
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6362
AvaI C'yCGr_G
Cuts at: 275 2164 2864 3071 3382 275
Size: 1889 700 207 311 3255
Fragments arranged by size:
3255 1889 700 311 207
AvrII C'CTAG_G
Cuts at: 2022 2022
Size: 6362
BamHI G'GATC_C
Cuts at: 3387 3387
Size: 6362
BanI G'GyrC_C
Cuts at: 132 563 644 1573 2005 2838 3024 3378 3792
Size: 431 81 929 432 833 186 354 414
Cuts at: 3792 4040 4075 132
Size: 248 35 2419
Fragments arranged by size:
2419 929 833 432 431 414 354 248
186 81 35
BanII G_rGCy'C
Cuts at: 224 341 422 532 869 1101 2207 2323 2354
Size: 117 81 110 337 232 1106 116 31
Cuts at: 2354 2517 2572 2714 3061 3113 3143 3194 3239
Size: 163 55 142 347 52 30 51 45
Cuts at: 3239 3376 4406 224
Size: 137 1030 2180
Fragments arranged by size:
2180 1106 1030 347 337 232 163 142
137 117 116 110 81 55 52 51
45 31 30
BbeI G_GCGC'C
Cuts at: 136 567 1577 2842 3796 4044 136
Size: 431 1010 1265 954 248 2454
Fragments arranged by size:
2454 1265 1010 954 431 248
BbsI GAAGACnn'nnnn_
Cuts at: 1117 2600 1117
Size: 1483 4879
BbvCI CC'TCA_GC
Cuts at: 281 2394 2486 3215 281
Size: 2113 92 729 3428
Fragments arranged by size:
3428 2113 729 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1995 2029 2134 2168 2794 2828 3336
Size: 34 1871 34 105 34 626 34 508
Cuts at: 3336 3370 90
Size: 34 3082
Fragments arranged by size:
3082 1871 626 508 105 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1705 4260 5316 6362 1705
Size: 2555 1056 1046 1705
Fragments arranged by size:
2555 1705 1056 1046
BclI T'GATC_A
Cuts at: 3882 3882
Size: 6362
BglI GCCn_nnn'nGGC
Cuts at: 112 214 572 1744 1957 2645 112
Size: 102 358 1172 213 688 3829
Fragments arranged by size:
3829 1172 688 358 213 102
BglII A'GATC_T
Cuts at: 1544 2562 3877 5914 1544
Size: 1018 1315 2037 1992
Fragments arranged by size:
2037 1992 1315 1018
BlpI GC'TnA_GC
Cuts at: 392 2206 3474 392
Size: 1814 1268 3280
Fragments arranged by size:
3280 1814 1268
Bme1580I G_kGCm'C
Cuts at: 532 582 647 774 836 1101 1887 2323 2517
Size: 50 65 127 62 265 786 436 194
Cuts at: 2517 2558 2714 2863 2890 3194 3987 4080 5204
Size: 41 156 149 27 304 793 93 1124
Cuts at: 5204 532
Size: 1690
Fragments arranged by size:
1690 1124 793 786 436 304 265 194
156 149 127 93 65 62 50 41
27
BmgBI CAC'GTC
Cuts at: 2091 2419 2091
Size: 328 6034
BmrI ACTGGGnnnn_n'
Cuts at: 863 1233 2942 3197 3574 3655 3990 6134 863
Size: 370 1709 255 377 81 335 2144 1091
Fragments arranged by size:
2144 1709 1091 377 370 335 255 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2221 4729 2221
Size: 2508 3854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 1382 1447 1744 2311 2340 3251 3307 1345
Size: 37 65 297 567 29 911 56 4400
Fragments arranged by size:
4400 911 567 297 65 56 37 29
Bpu10I CC'TnA_GC
Cuts at: 281 2260 2394 2486 3215 281
Size: 1979 134 92 729 3428
Fragments arranged by size:
3428 1979 729 134 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 1981 2997 3538 4417 5131 5429 1693
Size: 288 1016 541 879 714 298 2626
Fragments arranged by size:
2626 1016 879 714 541 298 288
BsaI GGTCTCn'nnnn_
Cuts at: 2334 3119 6066 2334
Size: 785 2947 2630
Fragments arranged by size:
2947 2630 785
BsaAI yAC'GTr
Cuts at: 3715 3715
Size: 6362
BsaBI GATnn'nnATC
Cuts at: 6 3876 5913 6
Size: 3870 2037 455
Fragments arranged by size:
3870 2037 455
BsaHI Gr'CG_yC
Cuts at: 133 564 1574 2839 3793 4041 4743 6239 133
Size: 431 1010 1265 954 248 702 1496 256
Fragments arranged by size:
1496 1265 1010 954 702 431 256 248
BsaWI w'CCGG_w
Cuts at: 407 737 1157 3551 4072 4750 5161 5308 5582
Size: 330 420 2394 521 678 411 147 274
Cuts at: 5582 407
Size: 1187
Fragments arranged by size:
2394 1187 678 521 420 411 330 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1453 1483 2239 2269 2842 2872 1453
Size: 30 756 30 573 30 4943
Fragments arranged by size:
4943 756 573 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2308 2376 3002 3152 2308
Size: 68 626 150 5518
Fragments arranged by size:
5518 626 150 68
BseYI C'CCAG_C
Cuts at: 198 889 1434 2224 2334 2340 2713 2889 2961
Size: 691 545 790 110 6 373 176 72
Cuts at: 2961 3045 3142 3787 4545 5210 5618 198
Size: 84 97 645 758 665 408 942
Fragments arranged by size:
942 790 758 691 665 645 545 408
373 176 110 97 84 72 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1542 1810 1899 2125 2364 2845 1542
Size: 268 89 226 239 481 5059
Fragments arranged by size:
5059 481 268 239 226 89
BsiEI CG_ry'CG
Cuts at: 104 3904 3950 5180 104
Size: 3800 46 1230 1286
Fragments arranged by size:
3800 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 341 377 629 856 1997 2015 2207 3061 3376
Size: 36 252 227 1141 18 192 854 315
Cuts at: 3376 4154 4344 5204 341
Size: 778 190 860 1499
Fragments arranged by size:
1499 1141 860 854 778 315 252 227
192 190 36 18
BsmBI CGTCTCn'nnnn_
Cuts at: 3056 3056
Size: 6362
Bsp1286I G_dGCh'C
Cuts at: 224 341 377 422 532 582 629 647 774
Size: 117 36 45 110 50 47 18 127
Cuts at: 774 836 856 869 1101 1887 1997 2015 2207
Size: 62 20 13 232 786 110 18 192
Cuts at: 2207 2323 2354 2517 2558 2572 2714 2863 2890
Size: 116 31 163 41 14 142 149 27
Cuts at: 2890 3061 3113 3143 3194 3239 3376 3987 4080
Size: 171 52 30 51 45 137 611 93
Cuts at: 4080 4154 4344 4406 5204 224
Size: 74 190 62 798 1382
Fragments arranged by size:
1382 798 786 611 232 192 190 171
163 149 142 137 127 117 116 110
110 93 74 62 62 52 51 50
47 45 45 41 36 31 30 27
20 18 18 14 13
BspEI T'CCGG_A
Cuts at: 737 737
Size: 6362
BspHI T'CATG_A
Cuts at: 4794 4794
Size: 6362
BspMI ACCTGCnnnn'nnnn_
Cuts at: 656 1070 1676 1832 2058 2769 3412 3626 3928
Size: 414 606 156 226 711 643 214 302
Cuts at: 3928 4309 656
Size: 381 2709
Fragments arranged by size:
2709 711 643 606 414 381 302 226
214 156
BsrBI CCG'CTC
Cuts at: 50 416 2264 2687 4654 4708 6192 6357 50
Size: 366 1848 423 1967 54 1484 165 55
Fragments arranged by size:
1967 1848 1484 423 366 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4274 4274
Size: 6362
BsrFI r'CCGG_y
Cuts at: 111 560 605 1157 4360 4541 111
Size: 449 45 552 3203 181 1932
Fragments arranged by size:
3203 1932 552 449 181 45
BssHII G'CGCG_C
Cuts at: 1803 1803
Size: 6362
BssSI C'ACGA_G
Cuts at: 4633 5341 4633
Size: 708 5654
BstAPI GCAn_nnn'nTGC
Cuts at: 1719 2124 2784 3739 1719
Size: 405 660 955 4342
Fragments arranged by size:
4342 955 660 405
BstBI TT'CG_AA
Cuts at: 4723 4723
Size: 6362
BstXI CCAn_nnnn'nTGG
Cuts at: 3316 3316
Size: 6362
BstYI r'GATC_y
Cuts at: 1 353 1544 2562 3387 3854 3877 4212 4458
Size: 352 1191 1018 825 467 23 335 246
Cuts at: 4458 4764 4776 4862 5914 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1191 1052 1018 825 467 449 352 335
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3120 3120
Size: 6362
BtgI C'CryG_G
Cuts at: 230 879 908 1147 1178 1869 1985 2324 2509
Size: 649 29 239 31 691 116 339 185
Cuts at: 2509 2545 2706 3309 230
Size: 36 161 603 3283
Fragments arranged by size:
3283 691 649 603 339 239 185 161
116 36 31 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 144 1026 1797 1961 4312 4493 4656 144
Size: 882 771 164 2351 181 163 1850
Fragments arranged by size:
2351 1850 882 771 181 164 163
BtsI GCAGTG_nn'
Cuts at: 1189 2125 3605 1189
Size: 936 1480 3946
Fragments arranged by size:
3946 1480 936
DraI TTT'AAA
Cuts at: 3361 3361
Size: 6362
DraIII CAC_nnn'GTG
Cuts at: 1590 1590
Size: 6362
DrdI GACnn_nn'nnGTC
Cuts at: 2769 4068 5412 2769
Size: 1299 1344 3719
Fragments arranged by size:
3719 1344 1299
EaeI y'GGCC_r
Cuts at: 113 558 573 905 1199 1251 1377 1610 1862
Size: 445 15 332 294 52 126 233 252
Cuts at: 1862 3947 4121 4512 4539 5884 113
Size: 2085 174 391 27 1345 591
Fragments arranged by size:
2085 1345 591 445 391 332 294 252
233 174 126 52 27 15
EagI C'GGCC_G
Cuts at: 3947 3947
Size: 6362
EarI CTCTTCn'nnn_
Cuts at: 229 406 487 925 4385 4595 229
Size: 177 81 438 3460 210 1996
Fragments arranged by size:
3460 1996 438 210 177 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 334 627 1512 4570 5312 5458 6172 334
Size: 293 885 3058 742 146 714 524
Fragments arranged by size:
3058 885 742 714 524 293 146
EcoICRI GAG'CTC
Cuts at: 339 2205 3059 3374 339
Size: 1866 854 315 3327
Fragments arranged by size:
3327 1866 854 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 1345 1382 1447 1744 2166 2311 2340 3251
Size: 216 37 65 297 422 145 29 911
Cuts at: 3251 3307 4187 4619 4972 5634 1129
Size: 56 880 432 353 662 1857
Fragments arranged by size:
1857 911 880 662 432 422 353 297
216 145 65 56 37 29
EcoNI CCTnn'n_nnAGG
Cuts at: 1828 2026 2441 3119 1828
Size: 198 415 678 5071
Fragments arranged by size:
5071 678 415 198
EcoO109I rG'GnC_Cy
Cuts at: 123 363 528 529 915 1097 1836 1959 2319
Size: 240 165 1 386 182 739 123 360
Cuts at: 2319 2320 2514 3007 3040 3255 3273 3501 123
Size: 1 194 493 33 215 18 228 2984
Fragments arranged by size:
2984 739 493 386 360 240 228 215
194 182 165 123 33 18 1 1
EcoRI G'AATT_C
Cuts at: 3366 3366
Size: 6362
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 765 4381 765
Size: 3616 2746
FspI TGC'GCA
Cuts at: 1453 4143 1453
Size: 2690 3672
HaeII r_GCGC'y
Cuts at: 136 567 1051 1178 1460 1577 2842 3796 4044
Size: 431 484 127 282 117 1265 954 248
Cuts at: 4044 5274 6045 136
Size: 1230 771 453
Fragments arranged by size:
1265 1230 954 771 484 453 431 282
248 127 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1147 1179 2239 2271 2338 2370 3118 3150 4204
Size: 32 1060 32 67 32 748 32 1054
Cuts at: 4204 4234 4236 4266 1147
Size: 30 2 30 3243
Fragments arranged by size:
3243 1060 1054 748 67 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1083 3401 1083
Size: 2318 4044
HindIII A'AGCT_T
Cuts at: 2746 2746
Size: 6362
KasI G'GCGC_C
Cuts at: 132 563 1573 2838 3792 4040 132
Size: 431 1010 1265 954 248 2454
Fragments arranged by size:
2454 1265 1010 954 431 248
KpnI G_GTAC'C
Cuts at: 3382 3382
Size: 6362
MluI A'CGCG_T
Cuts at: 5795 5795
Size: 6362
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5121 5305 5121
Size: 184 6178
MscI TGG'CCA
Cuts at: 1253 4123 1253
Size: 2870 3492
MslI CAynn'nnrTG
Cuts at: 958 1201 1501 1816 1990 4351 4478 958
Size: 243 300 315 174 2361 127 2842
Fragments arranged by size:
2842 2361 315 300 243 174 127
MspA1I CmG'CkG
Cuts at: 294 881 1149 1257 1360 1845 1871 2130 2344
Size: 587 268 108 103 485 26 259 214
Cuts at: 2344 2398 2965 3473 3787 4147 4931 5176 294
Size: 54 567 508 314 360 784 245 1480
Fragments arranged by size:
1480 784 587 567 508 485 360 314
268 259 245 214 108 103 54 26
NaeI GCC'GGC
Cuts at: 113 562 4543 113
Size: 449 3981 1932
Fragments arranged by size:
3981 1932 449
NarI GG'CG_CC
Cuts at: 133 564 1574 2839 3793 4041 133
Size: 431 1010 1265 954 248 2454
Fragments arranged by size:
2454 1265 1010 954 431 248
NcoI C'CATG_G
Cuts at: 1985 2545 2706 3309 1985
Size: 560 161 603 5038
Fragments arranged by size:
5038 603 560 161
NgoMIV G'CCGG_C
Cuts at: 111 560 4541 111
Size: 449 3981 1932
Fragments arranged by size:
3981 1932 449
NspI r_CATG'y
Cuts at: 1072 1629 1722 3415 3915 5518 1072
Size: 557 93 1693 500 1603 1916
Fragments arranged by size:
1916 1693 1603 557 500 93
PasI CC'CwG_GG
Cuts at: 2573 3035 3269 2573
Size: 462 234 5666
Fragments arranged by size:
5666 462 234
PciI A'CATG_T
Cuts at: 5514 5514
Size: 6362
PflMI CCAn_nnn'nTGG
Cuts at: 2546 3285 2546
Size: 739 5623
PfoI T'CCnGG_A
Cuts at: 149 437 149
Size: 288 6074
PmeI GTTT'AAAC
Cuts at: 3361 3361
Size: 6362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4808 4840 4808
Size: 32 6330
PpuMI rG'GwC_Cy
Cuts at: 915 1836 3040 3273 915
Size: 921 1204 233 4004
Fragments arranged by size:
4004 1204 921 233
PshAI GACnn'nnGTC
Cuts at: 2805 2805
Size: 6362
PspOMI G'GGCC_C
Cuts at: 528 1097 2319 2513 2710 3190 528
Size: 569 1222 194 197 480 3700
Fragments arranged by size:
3700 1222 569 480 197 194
PspXI vC'TCGA_Gb
Cuts at: 3071 3071
Size: 6362
PstI C_TGCA'G
Cuts at: 653 1307 1396 1784 1844 1958 2120 2129 2241
Size: 654 89 388 60 114 162 9 112
Cuts at: 2241 2789 2807 2915 2957 3409 4094 653
Size: 548 18 108 42 452 685 2921
Fragments arranged by size:
2921 685 654 548 452 388 162 114
112 108 89 60 42 18 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6362
PvuII CAG'CTG
Cuts at: 1360 1845 2130 2344 2398 2965 3787 4147 1360
Size: 485 285 214 54 567 822 360 3575
Fragments arranged by size:
3575 822 567 485 360 285 214 54
RsrII CG'GwC_CG
Cuts at: 4557 4557
Size: 6362
SacI G_AGCT'C
Cuts at: 341 2207 3061 3376 341
Size: 1866 854 315 3327
Fragments arranged by size:
3327 1866 854 315
SacII CC_GC'GG
Cuts at: 882 1150 1872 882
Size: 268 722 5372
Fragments arranged by size:
5372 722 268
SalI G'TCGA_C
Cuts at: 3399 3399
Size: 6362
SanDI GG'GwC_CC
Cuts at: 3040 3273 3040
Size: 233 6129
SapI GCTCTTCn'nnn_
Cuts at: 229 406 4385 4595 229
Size: 177 3979 210 1996
Fragments arranged by size:
3979 1996 210 177
SbfI CC_TGCA'GG
Cuts at: 1958 3409 1958
Size: 1451 4911
ScaI AGT'ACT
Cuts at: 1729 5879 1729
Size: 4150 2212
SexAI A'CCwGG_T
Cuts at: 1021 1340 1021
Size: 319 6043
SfcI C'TryA_G
Cuts at: 33 649 837 1303 1392 1780 1840 1954 2116
Size: 616 188 466 89 388 60 114 162
Cuts at: 2116 2125 2237 2785 2803 2911 2953 3405 4090
Size: 9 112 548 18 108 42 452 685
Cuts at: 4090 5058 5249 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 616 548 466 452 388
191 188 162 114 112 108 89 60
42 18 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2645 2645
Size: 6362
SfoI GGC'GCC
Cuts at: 134 565 1575 2840 3794 4042 134
Size: 431 1010 1265 954 248 2454
Fragments arranged by size:
2454 1265 1010 954 431 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6362
SmaI CCC'GGG
Cuts at: 277 3384 277
Size: 3107 3255
SmlI C'TyrA_G
Cuts at: 1708 1996 2976 3071 3517 4432 5146 5408 1708
Size: 288 980 95 446 915 714 262 2662
Fragments arranged by size:
2662 980 915 714 446 288 262 95
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6362
SphI G_CATG'C
Cuts at: 1722 3415 3915 1722
Size: 1693 500 4169
Fragments arranged by size:
4169 1693 500
StyI C'CwwG_G
Cuts at: 662 1941 1985 2022 2545 2706 3309 3496 3850
Size: 1279 44 37 523 161 603 187 354
Cuts at: 3850 662
Size: 3174
Fragments arranged by size:
3174 1279 603 523 354 187 161 44
37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2209 3945 4078 4537 4746 2209
Size: 1736 133 459 209 3825
Fragments arranged by size:
3825 1736 459 209 133
TatI w'GTAC_w
Cuts at: 719 1088 1169 1727 5877 719
Size: 369 81 558 4150 1204
Fragments arranged by size:
4150 1204 558 369 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2842 2874 5879 5911 2842
Size: 32 3005 32 3293
Fragments arranged by size:
3293 3005 32 32
Tth111I GACn'n_nGTC
Cuts at: 4159 4159
Size: 6362
XbaI T'CTAG_A
Cuts at: 3393 3393
Size: 6362
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 816 3857 816
Size: 3041 3321
XhoI C'TCGA_G
Cuts at: 3071 3071
Size: 6362
XmaI C'CCGG_G
Cuts at: 275 3382 275
Size: 3107 3255
XmnI GAAnn'nnTTC
Cuts at: 145 1546 145
Size: 1401 4961
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI BaeI BfrBI
BmtI BsiWI BsmI BsrGI BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PsiI PsrI SgrAI SnaBI
SrfI SspI StuI SwaI ZraI