Restriction map for
pF1KA1286
(Circular) (Six-base) MAPSORT of: pF1KA1286.seq Check: 4105 from: 1 to: 6728
pF1KA1286 6728 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2243 3636 2243
Size: 1393 5335
AccI GT'mk_AC
Cuts at: 236 671 3766 236
Size: 435 3095 3198
Fragments arranged by size:
3198 3095 435
Acc65I G'GTAC_C
Cuts at: 2857 3744 2857
Size: 887 5841
AclI AA'CG_TT
Cuts at: 6568 6568
Size: 6728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 563 1019 1366 1774 2362 3349 3484 4553
Size: 249 456 347 408 588 987 135 1069
Cuts at: 4553 4985 5338 6000 314
Size: 432 353 662 1042
Fragments arranged by size:
1069 1042 987 662 588 456 432 408
353 347 249 135
AflIII A'CryG_T
Cuts at: 1159 4080 5880 6161 1159
Size: 2921 1800 281 1726
Fragments arranged by size:
2921 1800 1726 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2251 3658 2251
Size: 1407 5321
AlwNI CAG_nnn'CTG
Cuts at: 2451 3047 3493 4033 5471 2451
Size: 596 446 540 1438 3708
Fragments arranged by size:
3708 1438 596 540 446
ApaI G_GGCC'C
Cuts at: 720 972 2319 2397 2525 3695 720
Size: 252 1347 78 128 1170 3753
Fragments arranged by size:
3753 1347 1170 252 128 78
ApaLI G'TGCA_C
Cuts at: 1163 5566 1163
Size: 4403 2325
ApoI r'AATT_y
Cuts at: 1990 3732 6081 6337 1990
Size: 1742 2349 256 2381
Fragments arranged by size:
2381 2349 1742 256
AseI AT'TA_AT
Cuts at: 20 2693 20
Size: 2673 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6728
AvaI C'yCGr_G
Cuts at: 191 2218 2239 3236 3407 3748 191
Size: 2027 21 997 171 341 3171
Fragments arranged by size:
3171 2027 997 341 171 21
BamHI G'GATC_C
Cuts at: 992 1932 3753 992
Size: 940 1821 3967
Fragments arranged by size:
3967 1821 940
BanI G'GyrC_C
Cuts at: 937 1222 2857 3744 4158 4406 4441 937
Size: 285 1635 887 414 248 35 3224
Fragments arranged by size:
3224 1635 887 414 285 248 35
BanII G_rGCy'C
Cuts at: 720 972 1181 1199 2319 2397 2525 2627 2675
Size: 252 209 18 1120 78 128 102 48
Cuts at: 2675 2753 3695 3742 4772 720
Size: 78 942 47 1030 2676
Fragments arranged by size:
2676 1120 1030 942 252 209 128 102
78 78 48 47 18
BbeI G_GCGC'C
Cuts at: 941 4162 4410 941
Size: 3221 248 3259
Fragments arranged by size:
3259 3221 248
BbsI GAAGACnn'nnnn_
Cuts at: 551 746 1735 2264 2288 2579 3320 3590 551
Size: 195 989 529 24 291 741 270 3689
Fragments arranged by size:
3689 989 741 529 291 270 195 24
BciVI GTATCCnnnnn_n'
Cuts at: 2508 4626 5682 6728 2508
Size: 2118 1056 1046 2508
Fragments arranged by size:
2508 2118 1056 1046
BclI T'GATC_A
Cuts at: 3386 4248 3386
Size: 862 5866
BfrBI ATG'CAT
Cuts at: 427 427
Size: 6728
BglI GCCn_nnn'nGGC
Cuts at: 1220 2520 2631 1220
Size: 1300 111 5317
Fragments arranged by size:
5317 1300 111
BglII A'GATC_T
Cuts at: 1565 4243 6280 1565
Size: 2678 2037 2013
Fragments arranged by size:
2678 2037 2013
BlpI GC'TnA_GC
Cuts at: 1706 3840 1706
Size: 2134 4594
Bme1580I G_kGCm'C
Cuts at: 635 720 965 972 1167 1442 2230 2319 2397
Size: 85 245 7 195 275 788 89 78
Cuts at: 2397 2525 3245 3695 4353 4446 5570 635
Size: 128 720 450 658 93 1124 1793
Fragments arranged by size:
1793 1124 788 720 658 450 275 245
195 128 93 89 85 78 7
BmgBI CAC'GTC
Cuts at: 2286 3459 2286
Size: 1173 5555
BmrI ACTGGGnnnn_n'
Cuts at: 975 2639 3940 4021 4356 6500 975
Size: 1664 1301 81 335 2144 1203
Fragments arranged by size:
2144 1664 1301 1203 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 134 1281 1846 2246 3036 3520 5095 134
Size: 1147 565 400 790 484 1575 1767
Fragments arranged by size:
1767 1575 1147 790 565 484 400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1894 2056 2374 2566 2641 2971 2978 3515 1894
Size: 162 318 192 75 330 7 537 5107
Fragments arranged by size:
5107 537 330 318 192 162 75 7
Bpu10I CC'TnA_GC
Cuts at: 3285 3676 3285
Size: 391 6337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 700 1391 1414 1986 3586 3904 4783 5497
Size: 540 691 23 572 1600 318 879 714
Cuts at: 5497 5795 160
Size: 298 1093
Fragments arranged by size:
1600 1093 879 714 691 572 540 318
298 23
BsaI GGTCTCn'nnnn_
Cuts at: 190 1582 3163 3317 6432 190
Size: 1392 1581 154 3115 486
Fragments arranged by size:
3115 1581 1392 486 154
BsaAI yAC'GTr
Cuts at: 4081 4081
Size: 6728
BsaBI GATnn'nnATC
Cuts at: 6 4242 6279 6
Size: 4236 2037 455
Fragments arranged by size:
4236 2037 455
BsaHI Gr'CG_yC
Cuts at: 249 842 938 3199 4159 4407 5109 6605 249
Size: 593 96 2261 960 248 702 1496 372
Fragments arranged by size:
2261 1496 960 702 593 372 248 96
BsaWI w'CCGG_w
Cuts at: 3917 4438 5116 5527 5674 5948 3917
Size: 521 678 411 147 274 4697
Fragments arranged by size:
4697 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1495 1525 1741 1771 2257 2287 3036 3066 3331
Size: 30 216 30 486 30 749 30 265
Cuts at: 3331 3361 1495
Size: 30 4862
Fragments arranged by size:
4862 749 486 265 216 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 595 1378 1762 2077 2086 2578 2597 595
Size: 783 384 315 9 492 19 4726
Fragments arranged by size:
4726 783 492 384 315 19 9
BseYI C'CCAG_C
Cuts at: 218 457 1174 3077 3468 4153 4911 5576 5984
Size: 239 717 1903 391 685 758 665 408
Cuts at: 5984 218
Size: 962
Fragments arranged by size:
1903 962 758 717 685 665 408 391
239
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1130 1757 1922 2356 2621 3406 3664 1130
Size: 627 165 434 265 785 258 4194
Fragments arranged by size:
4194 785 627 434 265 258 165
BsiEI CG_ry'CG
Cuts at: 104 2855 4270 4316 5546 104
Size: 2751 1415 46 1230 1286
Fragments arranged by size:
2751 1415 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 184 1167 2753 3742 4520 4710 5570 184
Size: 983 1586 989 778 190 860 1342
Fragments arranged by size:
1586 1342 989 983 860 778 190
BsmI GAATG_Cn'
Cuts at: 144 331 1147 144
Size: 187 816 5725
Fragments arranged by size:
5725 816 187
Bsp1286I G_dGCh'C
Cuts at: 184 635 720 965 972 1167 1181 1199 1442
Size: 451 85 245 7 195 14 18 243
Cuts at: 1442 2230 2319 2397 2525 2627 2675 2753 3245
Size: 788 89 78 128 102 48 78 492
Cuts at: 3245 3695 3742 4353 4446 4520 4710 4772 5570
Size: 450 47 611 93 74 190 62 798
Cuts at: 5570 184
Size: 1342
Fragments arranged by size:
1342 798 788 611 492 451 450 245
243 195 190 128 102 93 89 85
78 78 74 62 48 47 18 14
7
BspHI T'CATG_A
Cuts at: 1184 5160 1184
Size: 3976 2752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1087 1833 1925 2243 2705 3636 3778 3992 4294
Size: 746 92 318 462 931 142 214 302
Cuts at: 4294 4675 1087
Size: 381 3140
Fragments arranged by size:
3140 931 746 462 381 318 302 214
142 92
BsrBI CCG'CTC
Cuts at: 50 1788 5020 5074 6558 6723 50
Size: 1738 3232 54 1484 165 55
Fragments arranged by size:
3232 1738 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 792 4640 792
Size: 3848 2880
BsrFI r'CCGG_y
Cuts at: 149 674 2854 4726 4907 149
Size: 525 2180 1872 181 1970
Fragments arranged by size:
2180 1970 1872 525 181
BsrGI T'GTAC_A
Cuts at: 822 822
Size: 6728
BssSI C'ACGA_G
Cuts at: 4999 5707 4999
Size: 708 6020
BstAPI GCAn_nnn'nTGC
Cuts at: 920 2556 2799 4105 920
Size: 1636 243 1306 3543
Fragments arranged by size:
3543 1636 1306 243
BstBI TT'CG_AA
Cuts at: 5089 5089
Size: 6728
BstXI CCAn_nnnn'nTGG
Cuts at: 1872 2007 2736 3720 1872
Size: 135 729 984 4880
Fragments arranged by size:
4880 984 729 135
BstYI r'GATC_y
Cuts at: 1 992 1565 1932 3753 4220 4243 4578 4824
Size: 991 573 367 1821 467 23 335 246
Cuts at: 4824 5130 5142 5228 6280 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1821 1052 991 573 467 449 367 335
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 857 857
Size: 6728
BtgI C'CryG_G
Cuts at: 1865 2967 3216 3318 3590 1865
Size: 1102 249 102 272 5003
Fragments arranged by size:
5003 1102 272 249 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1346 3679 4678 4859 5022 1346
Size: 2333 999 181 163 3052
Fragments arranged by size:
3052 2333 999 181 163
BtsI GCAGTG_nn'
Cuts at: 717 1283 1745 2767 3195 3550 3971 717
Size: 566 462 1022 428 355 421 3474
Fragments arranged by size:
3474 1022 566 462 428 421 355
ClaI AT'CG_AT
Cuts at: 734 3392 734
Size: 2658 4070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 915 950 915
Size: 35 6693
DraI TTT'AAA
Cuts at: 3727 3727
Size: 6728
DraIII CAC_nnn'GTG
Cuts at: 1276 2869 1276
Size: 1593 5135
DrdI GACnn_nn'nnGTC
Cuts at: 4434 5778 4434
Size: 1344 5384
EaeI y'GGCC_r
Cuts at: 1280 2852 3031 4313 4487 4878 4905 6250 1280
Size: 1572 179 1282 174 391 27 1345 1758
Fragments arranged by size:
1758 1572 1345 1282 391 179 174 27
EagI C'GGCC_G
Cuts at: 2852 4313 2852
Size: 1461 5267
EarI CTCTTCn'nnn_
Cuts at: 802 1186 1367 1373 1388 1813 2902 3005 3083
Size: 384 181 6 15 425 1089 103 78
Cuts at: 3083 3511 3523 4751 4961 802
Size: 428 12 1228 210 2569
Fragments arranged by size:
2569 1228 1089 428 425 384 210 181
103 78 15 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2043 2137 2401 2600 4936 5678 5824 6538 2043
Size: 94 264 199 2336 742 146 714 2233
Fragments arranged by size:
2336 2233 742 714 264 199 146 94
EcoICRI GAG'CTC
Cuts at: 2751 3740 2751
Size: 989 5739
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 563 1019 1366 1774 1894 2056 2362 2374
Size: 249 456 347 408 120 162 306 12
Cuts at: 2374 2566 2641 2971 2978 3349 3484 3515 4553
Size: 192 75 330 7 371 135 31 1038
Cuts at: 4553 4985 5338 6000 314
Size: 432 353 662 1042
Fragments arranged by size:
1042 1038 662 456 432 408 371 353
347 330 306 249 192 162 135 120
75 31 12 7
EcoNI CCTnn'n_nnAGG
Cuts at: 1551 1587 1921 1551
Size: 36 334 6358
Fragments arranged by size:
6358 334 36
EcoO109I rG'GnC_Cy
Cuts at: 585 716 2157 2393 2521 2522 2946 3071 3414
Size: 131 1441 236 128 1 424 125 343
Cuts at: 3414 3691 3692 3867 585
Size: 277 1 175 3446
Fragments arranged by size:
3446 1441 424 343 277 236 175 131
128 125 1 1
EcoRI G'AATT_C
Cuts at: 1990 3732 1990
Size: 1742 4986
EcoRV GAT'ATC
Cuts at: 318 318
Size: 6728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4747 4747
Size: 6728
FspI TGC'GCA
Cuts at: 1240 4509 1240
Size: 3269 3459
HaeII r_GCGC'y
Cuts at: 941 1674 2378 3018 4162 4410 5640 6411 941
Size: 733 704 640 1144 248 1230 771 1258
Fragments arranged by size:
1258 1230 1144 771 733 704 640 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 673 705 1495 1527 1829 1861 2129 2156 2161
Size: 32 790 32 302 32 268 27 5
Cuts at: 2161 2188 2644 2676 2816 2848 3378 3410 4570
Size: 27 456 32 140 32 530 32 1160
Cuts at: 4570 4600 4602 4632 673
Size: 30 2 30 2769
Fragments arranged by size:
2769 1160 790 530 456 302 268 140
32 32 32 32 32 32 30 30
27 27 5 2
HincII GTy'rAC
Cuts at: 1962 3767 1962
Size: 1805 4923
HindIII A'AGCT_T
Cuts at: 3182 3182
Size: 6728
KasI G'GCGC_C
Cuts at: 937 4158 4406 937
Size: 3221 248 3259
Fragments arranged by size:
3259 3221 248
KpnI G_GTAC'C
Cuts at: 2861 3748 2861
Size: 887 5841
MluI A'CGCG_T
Cuts at: 6161 6161
Size: 6728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 103 5487 5671 103
Size: 5384 184 1160
Fragments arranged by size:
5384 1160 184
MscI TGG'CCA
Cuts at: 4489 4489
Size: 6728
MslI CAynn'nnrTG
Cuts at: 4717 4844 4717
Size: 127 6601
MspA1I CmG'CkG
Cuts at: 222 504 878 1067 1170 1611 2143 2340 2539
Size: 282 374 189 103 441 532 197 199
Cuts at: 2539 3320 3453 3839 4153 4513 5297 5542 222
Size: 781 133 386 314 360 784 245 1408
Fragments arranged by size:
1408 784 781 532 441 386 374 360
314 282 245 199 197 189 133 103
NaeI GCC'GGC
Cuts at: 151 4909 151
Size: 4758 1970
NarI GG'CG_CC
Cuts at: 938 4159 4407 938
Size: 3221 248 3259
Fragments arranged by size:
3259 3221 248
NcoI C'CATG_G
Cuts at: 1865 2967 1865
Size: 1102 5626
NdeI CA'TA_TG
Cuts at: 207 207
Size: 6728
NgoMIV G'CCGG_C
Cuts at: 149 4907 149
Size: 4758 1970
NsiI A_TGCA'T
Cuts at: 429 429
Size: 6728
NspI r_CATG'y
Cuts at: 427 1163 3781 4281 5884 427
Size: 736 2618 500 1603 1271
Fragments arranged by size:
2618 1603 1271 736 500
PasI CC'CwG_GG
Cuts at: 2526 2526
Size: 6728
PciI A'CATG_T
Cuts at: 1159 5880 1159
Size: 4721 2007
PflMI CCAn_nnn'nTGG
Cuts at: 2869 2973 2869
Size: 104 6624
PfoI T'CCnGG_A
Cuts at: 2948 3022 3248 2948
Size: 74 226 6428
Fragments arranged by size:
6428 226 74
PmeI GTTT'AAAC
Cuts at: 3727 3727
Size: 6728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3253 3285 5174 5206 3253
Size: 32 1889 32 4775
Fragments arranged by size:
4775 1889 32 32
PpuMI rG'GwC_Cy
Cuts at: 585 2157 2946 3071 585
Size: 1572 789 125 4242
Fragments arranged by size:
4242 1572 789 125
PspOMI G'GGCC_C
Cuts at: 716 968 2315 2393 2521 3691 716
Size: 252 1347 78 128 1170 3753
Fragments arranged by size:
3753 1347 1170 252 128 78
PstI C_TGCA'G
Cuts at: 651 890 1691 1925 2774 3775 4460 651
Size: 239 801 234 849 1001 685 2919
Fragments arranged by size:
2919 1001 849 801 685 239 234
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6728
PvuII CAG'CTG
Cuts at: 2143 2340 2539 4153 4513 2143
Size: 197 199 1614 360 4358
Fragments arranged by size:
4358 1614 360 199 197
RsrII CG'GwC_CG
Cuts at: 4923 4923
Size: 6728
SacI G_AGCT'C
Cuts at: 2753 3742 2753
Size: 989 5739
SacII CC_GC'GG
Cuts at: 3321 3321
Size: 6728
SalI G'TCGA_C
Cuts at: 3765 3765
Size: 6728
SapI GCTCTTCn'nnn_
Cuts at: 1186 1388 3005 3511 4751 4961 1186
Size: 202 1617 506 1240 210 2953
Fragments arranged by size:
2953 1617 1240 506 210 202
SbfI CC_TGCA'GG
Cuts at: 3775 3775
Size: 6728
ScaI AGT'ACT
Cuts at: 6245 6245
Size: 6728
SexAI A'CCwGG_T
Cuts at: 1272 2366 3719 1272
Size: 1094 1353 4281
Fragments arranged by size:
4281 1353 1094
SfcI C'TryA_G
Cuts at: 33 168 647 886 1569 1687 1921 2770 2926
Size: 135 479 239 683 118 234 849 156
Cuts at: 2926 3048 3771 4456 5424 5615 33
Size: 122 723 685 968 191 1146
Fragments arranged by size:
1146 968 849 723 685 683 479 239
234 191 156 135 122 118
SfoI GGC'GCC
Cuts at: 939 4160 4408 939
Size: 3221 248 3259
Fragments arranged by size:
3259 3221 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6728
SmaI CCC'GGG
Cuts at: 193 3409 3750 193
Size: 3216 341 3171
Fragments arranged by size:
3216 3171 341
SmlI C'TyrA_G
Cuts at: 175 679 1406 1429 1965 3236 3601 3883 4798
Size: 504 727 23 536 1271 365 282 915
Cuts at: 4798 5512 5774 175
Size: 714 262 1129
Fragments arranged by size:
1271 1129 915 727 714 536 504 365
282 262 23
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6728
SphI G_CATG'C
Cuts at: 427 3781 4281 427
Size: 3354 500 2874
Fragments arranged by size:
3354 2874 500
StuI AGG'CCT
Cuts at: 2554 2554
Size: 6728
StyI C'CwwG_G
Cuts at: 392 1865 2057 2967 3034 3335 3553 3572 3862
Size: 1473 192 910 67 301 218 19 290
Cuts at: 3862 4216 392
Size: 354 2904
Fragments arranged by size:
2904 1473 910 354 301 290 218 192
67 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 201 2262 3565 4311 4444 4903 5112 201
Size: 2061 1303 746 133 459 209 1817
Fragments arranged by size:
2061 1817 1303 746 459 209 133
TatI w'GTAC_w
Cuts at: 822 6243 822
Size: 5421 1307
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3036 3068 3331 3363 6245 6277 3036
Size: 32 263 32 2882 32 3487
Fragments arranged by size:
3487 2882 263 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2944 3562 4525 2944
Size: 618 963 5147
Fragments arranged by size:
5147 963 618
XbaI T'CTAG_A
Cuts at: 3759 3759
Size: 6728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 465 835 4223 465
Size: 370 3388 2970
Fragments arranged by size:
3388 2970 370
XhoI C'TCGA_G
Cuts at: 3236 3236
Size: 6728
XmaI C'CCGG_G
Cuts at: 191 3407 3748 191
Size: 3216 341 3171
Fragments arranged by size:
3216 3171 341
XmnI GAAnn'nnTTC
Cuts at: 3505 3505
Size: 6728
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BmtI BsiWI BsmBI BspEI
BssHII BstEII BstZ17I FseI FspAI HpaI MfeI NheI
NotI NruI PacI PmlI PshAI PsiI PspXI PsrI
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI