Restriction map for
pF1KA1287
(Circular) (Six-base) MAPSORT of: pF1KA1287.seq Check: 9761 from: 1 to: 6725
pF1KA1287 6725 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 285 285
Size: 6725
AccI GT'mk_AC
Cuts at: 2650 3303 3763 2650
Size: 653 460 5612
Fragments arranged by size:
5612 653 460
Acc65I G'GTAC_C
Cuts at: 3741 3741
Size: 6725
AclI AA'CG_TT
Cuts at: 6565 6565
Size: 6725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 457 722 1340 1499 2599 2815 2975 4550
Size: 321 265 618 159 1100 216 160 1575
Cuts at: 4550 4982 5335 5997 136
Size: 432 353 662 864
Fragments arranged by size:
1575 1100 864 662 618 432 353 321
265 216 160 159
AfeI AGC'GCT
Cuts at: 3027 3027
Size: 6725
AflIII A'CryG_T
Cuts at: 714 2744 3550 4077 5877 6158 714
Size: 2030 806 527 1800 281 1281
Fragments arranged by size:
2030 1800 1281 806 527 281
AhdI GACnn_n'nnGTC
Cuts at: 1358 1358
Size: 6725
AleI CACnn'nnGTG
Cuts at: 2416 2416
Size: 6725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3518 3518
Size: 6725
AlwNI CAG_nnn'CTG
Cuts at: 2314 4030 5468 2314
Size: 1716 1438 3571
Fragments arranged by size:
3571 1716 1438
ApaLI G'TGCA_C
Cuts at: 272 1265 5563 272
Size: 993 4298 1434
Fragments arranged by size:
4298 1434 993
ApoI r'AATT_y
Cuts at: 583 1679 1908 2176 2511 2531 3729 6078 6334
Size: 1096 229 268 335 20 1198 2349 256
Cuts at: 6334 583
Size: 974
Fragments arranged by size:
2349 1198 1096 974 335 268 256 229
20
AseI AT'TA_AT
Cuts at: 20 20
Size: 6725
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6725
AvaI C'yCGr_G
Cuts at: 3745 3745
Size: 6725
BamHI G'GATC_C
Cuts at: 3622 3645 3750 3622
Size: 23 105 6597
Fragments arranged by size:
6597 105 23
BanI G'GyrC_C
Cuts at: 727 3741 4155 4403 4438 727
Size: 3014 414 248 35 3014
Fragments arranged by size:
3014 3014 414 248 35
BanII G_rGCy'C
Cuts at: 303 671 1214 3739 4769 303
Size: 368 543 2525 1030 2259
Fragments arranged by size:
2525 2259 1030 543 368
BbeI G_GCGC'C
Cuts at: 4159 4407 4159
Size: 248 6477
BbsI GAAGACnn'nnnn_
Cuts at: 142 590 1666 2113 3062 3547 142
Size: 448 1076 447 949 485 3320
Fragments arranged by size:
3320 1076 949 485 448 447
BciVI GTATCCnnnnn_n'
Cuts at: 4623 5679 6725 4623
Size: 1056 1046 4623
Fragments arranged by size:
4623 1056 1046
BclI T'GATC_A
Cuts at: 2697 4245 2697
Size: 1548 5177
BfrBI ATG'CAT
Cuts at: 2893 3313 2893
Size: 420 6305
BglI GCCn_nnn'nGGC
Cuts at: 2586 2586
Size: 6725
BglII A'GATC_T
Cuts at: 4240 6277 4240
Size: 2037 4688
BlpI GC'TnA_GC
Cuts at: 1331 3143 3389 3837 1331
Size: 1812 246 448 4219
Fragments arranged by size:
4219 1812 448 246
Bme1580I G_kGCm'C
Cuts at: 276 1269 4350 4443 5567 276
Size: 993 3081 93 1124 1434
Fragments arranged by size:
3081 1434 1124 993 93
BmgBI CAC'GTC
Cuts at: 3553 3553
Size: 6725
BmrI ACTGGGnnnn_n'
Cuts at: 2395 3937 4018 4353 6497 2395
Size: 1542 81 335 2144 2623
Fragments arranged by size:
2623 2144 1542 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1379 5092 1379
Size: 3713 3012
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 1162 356
Size: 806 5919
Bpu10I CC'TnA_GC
Cuts at: 302 421 2906 3236 302
Size: 119 2485 330 3791
Fragments arranged by size:
3791 2485 330 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 1668 2180 3280 3901 4780 5494 5792 877
Size: 791 512 1100 621 879 714 298 1810
Fragments arranged by size:
1810 1100 879 791 714 621 512 298
BsaI GGTCTCn'nnnn_
Cuts at: 1090 3435 3593 6429 1090
Size: 2345 158 2836 1386
Fragments arranged by size:
2836 2345 1386 158
BsaAI yAC'GTr
Cuts at: 4078 4078
Size: 6725
BsaBI GATnn'nnATC
Cuts at: 6 1297 2170 4239 6276 6
Size: 1291 873 2069 2037 455
Fragments arranged by size:
2069 2037 1291 873 455
BsaHI Gr'CG_yC
Cuts at: 1520 4156 4404 5106 6602 1520
Size: 2636 248 702 1496 1643
Fragments arranged by size:
2636 1643 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1046 2862 3914 4435 5113 5524 5671 5945 1046
Size: 1816 1052 521 678 411 147 274 1826
Fragments arranged by size:
1826 1816 1052 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1848 1878 2228 2258 2844 2874 3012 3042 1848
Size: 30 350 30 586 30 138 30 5531
Fragments arranged by size:
5531 586 350 138 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 452 2974 452
Size: 2522 4203
BseYI C'CCAG_C
Cuts at: 296 1378 4150 4908 5573 5981 296
Size: 1082 2772 758 665 408 1040
Fragments arranged by size:
2772 1082 1040 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2936 2936
Size: 6725
BsiEI CG_ry'CG
Cuts at: 104 3194 4267 4313 5543 104
Size: 3090 1073 46 1230 1286
Fragments arranged by size:
3090 1286 1230 1073 46
BsiHKAI G_wGCw'C
Cuts at: 276 671 1269 2063 2790 3739 4517 4707 5567
Size: 395 598 794 727 949 778 190 860
Cuts at: 5567 276
Size: 1434
Fragments arranged by size:
1434 949 860 794 778 727 598 395
190
BsmI GAATG_Cn'
Cuts at: 1154 2226 2339 1154
Size: 1072 113 5540
Fragments arranged by size:
5540 1072 113
BsmBI CGTCTCn'nnnn_
Cuts at: 1527 2248 1527
Size: 721 6004
Bsp1286I G_dGCh'C
Cuts at: 276 303 671 1214 1269 2063 2790 3739 4350
Size: 27 368 543 55 794 727 949 611
Cuts at: 4350 4443 4517 4707 4769 5567 276
Size: 93 74 190 62 798 1434
Fragments arranged by size:
1434 949 798 794 727 611 543 368
190 93 74 62 55 27
BspEI T'CCGG_A
Cuts at: 1046 2862 1046
Size: 1816 4909
BspHI T'CATG_A
Cuts at: 5157 5157
Size: 6725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 285 1683 3187 3775 3989 4291 4672 285
Size: 1398 1504 588 214 302 381 2338
Fragments arranged by size:
2338 1504 1398 588 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2905 5017 5071 6555 6720 50
Size: 2855 2112 54 1484 165 55
Fragments arranged by size:
2855 2112 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 188 3122 3137 4637 188
Size: 2934 15 1500 2276
Fragments arranged by size:
2934 2276 1500 15
BsrFI r'CCGG_y
Cuts at: 4723 4904 4723
Size: 181 6544
BsrGI T'GTAC_A
Cuts at: 142 1901 3119 3654 142
Size: 1759 1218 535 3213
Fragments arranged by size:
3213 1759 1218 535
BssSI C'ACGA_G
Cuts at: 4996 5704 4996
Size: 708 6017
BstAPI GCAn_nnn'nTGC
Cuts at: 271 1341 4102 271
Size: 1070 2761 2894
Fragments arranged by size:
2894 2761 1070
BstBI TT'CG_AA
Cuts at: 5086 5086
Size: 6725
BstXI CCAn_nnnn'nTGG
Cuts at: 3584 3584
Size: 6725
BstYI r'GATC_y
Cuts at: 1 2673 3129 3622 3645 3750 4217 4240 4575
Size: 2672 456 493 23 105 467 23 335
Cuts at: 4575 4821 5127 5139 5225 6277 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2672 1052 493 467 456 449 335 306
246 105 86 23 23 12
BstZ17I GTA'TAC
Cuts at: 2651 3304 2651
Size: 653 6072
Bsu36I CC'TnA_GG
Cuts at: 2689 2689
Size: 6725
BtgI C'CryG_G
Cuts at: 374 2524 374
Size: 2150 4575
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4675 4856 5019 4675
Size: 181 163 6381
Fragments arranged by size:
6381 181 163
BtsI GCAGTG_nn'
Cuts at: 450 3968 450
Size: 3518 3207
DraI TTT'AAA
Cuts at: 3724 3724
Size: 6725
DraIII CAC_nnn'GTG
Cuts at: 271 1818 3331 271
Size: 1547 1513 3665
Fragments arranged by size:
3665 1547 1513
DrdI GACnn_nn'nnGTC
Cuts at: 4431 5775 4431
Size: 1344 5381
EaeI y'GGCC_r
Cuts at: 752 2900 4310 4484 4875 4902 6247 752
Size: 2148 1410 174 391 27 1345 1230
Fragments arranged by size:
2148 1410 1345 1230 391 174 27
EagI C'GGCC_G
Cuts at: 4310 4310
Size: 6725
EarI CTCTTCn'nnn_
Cuts at: 1238 2078 2096 2951 4748 4958 1238
Size: 840 18 855 1797 210 3005
Fragments arranged by size:
3005 1797 855 840 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2008 4933 5675 5821 6535 2008
Size: 2925 742 146 714 2198
Fragments arranged by size:
2925 2198 742 714 146
EcoICRI GAG'CTC
Cuts at: 669 3737 669
Size: 3068 3657
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 356 457 722 1162 1340 1499 2599 2815
Size: 220 101 265 440 178 159 1100 216
Cuts at: 2815 2975 4550 4982 5335 5997 136
Size: 160 1575 432 353 662 864
Fragments arranged by size:
1575 1100 864 662 440 432 353 265
220 216 178 160 159 101
EcoNI CCTnn'n_nnAGG
Cuts at: 1650 1650
Size: 6725
EcoO109I rG'GnC_Cy
Cuts at: 789 888 1719 3864 789
Size: 99 831 2145 3650
Fragments arranged by size:
3650 2145 831 99
EcoRI G'AATT_C
Cuts at: 2511 2531 3729 2511
Size: 20 1198 5507
Fragments arranged by size:
5507 1198 20
EcoRV GAT'ATC
Cuts at: 2758 2758
Size: 6725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 844 1683 2107 2797 4744 844
Size: 839 424 690 1947 2825
Fragments arranged by size:
2825 1947 839 690 424
FspI TGC'GCA
Cuts at: 4506 4506
Size: 6725
HaeII r_GCGC'y
Cuts at: 3029 4159 4407 5637 6408 3029
Size: 1130 248 1230 771 3346
Fragments arranged by size:
3346 1230 1130 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 440 472 942 974 1344 1376 2658 2661 2690
Size: 32 470 32 370 32 1282 3 29
Cuts at: 2690 2693 3457 3489 4567 4597 4599 4629 440
Size: 3 764 32 1078 30 2 30 2536
Fragments arranged by size:
2536 1282 1078 764 470 370 32 32
32 32 30 30 29 3 3 2
HincII GTy'rAC
Cuts at: 351 2486 3371 3764 351
Size: 2135 885 393 3312
Fragments arranged by size:
3312 2135 885 393
HindIII A'AGCT_T
Cuts at: 704 974 3145 704
Size: 270 2171 4284
Fragments arranged by size:
4284 2171 270
HpaI GTT'AAC
Cuts at: 2486 3371 2486
Size: 885 5840
KasI G'GCGC_C
Cuts at: 4155 4403 4155
Size: 248 6477
KpnI G_GTAC'C
Cuts at: 3745 3745
Size: 6725
MfeI C'AATT_G
Cuts at: 2834 2834
Size: 6725
MluI A'CGCG_T
Cuts at: 6158 6158
Size: 6725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 599 1459 2826 5484 5668 599
Size: 860 1367 2658 184 1656
Fragments arranged by size:
2658 1656 1367 860 184
MscI TGG'CCA
Cuts at: 754 4486 754
Size: 3732 2993
MslI CAynn'nnrTG
Cuts at: 910 2268 2416 4714 4841 910
Size: 1358 148 2298 127 2794
Fragments arranged by size:
2794 2298 1358 148 127
MspA1I CmG'CkG
Cuts at: 1378 1458 1848 2395 2920 3836 4150 4510 5294
Size: 80 390 547 525 916 314 360 784
Cuts at: 5294 5539 1378
Size: 245 2564
Fragments arranged by size:
2564 916 784 547 525 390 360 314
245 80
NaeI GCC'GGC
Cuts at: 4906 4906
Size: 6725
NarI GG'CG_CC
Cuts at: 4156 4404 4156
Size: 248 6477
NdeI CA'TA_TG
Cuts at: 2472 2472
Size: 6725
NgoMIV G'CCGG_C
Cuts at: 4904 4904
Size: 6725
NsiI A_TGCA'T
Cuts at: 2895 3315 2895
Size: 420 6305
NspI r_CATG'y
Cuts at: 718 1252 2748 3778 4278 5881 718
Size: 534 1496 1030 500 1603 1562
Fragments arranged by size:
1603 1562 1496 1030 534 500
PciI A'CATG_T
Cuts at: 714 2744 5877 714
Size: 2030 3133 1562
Fragments arranged by size:
3133 2030 1562
PflMI CCAn_nnn'nTGG
Cuts at: 937 937
Size: 6725
PmeI GTTT'AAAC
Cuts at: 3724 3724
Size: 6725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5171 5203 5171
Size: 32 6693
PpuMI rG'GwC_Cy
Cuts at: 1719 1719
Size: 6725
PsiI TTA'TAA
Cuts at: 3614 3614
Size: 6725
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1961 1993 1961
Size: 32 6693
PstI C_TGCA'G
Cuts at: 642 1346 2207 3772 4457 642
Size: 704 861 1565 685 2910
Fragments arranged by size:
2910 1565 861 704 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6725
PvuII CAG'CTG
Cuts at: 1378 1458 1848 2395 2920 4150 4510 1378
Size: 80 390 547 525 1230 360 3593
Fragments arranged by size:
3593 1230 547 525 390 360 80
RsrII CG'GwC_CG
Cuts at: 4920 4920
Size: 6725
SacI G_AGCT'C
Cuts at: 671 3739 671
Size: 3068 3657
SalI G'TCGA_C
Cuts at: 3762 3762
Size: 6725
SanDI GG'GwC_CC
Cuts at: 1719 1719
Size: 6725
SapI GCTCTTCn'nnn_
Cuts at: 1238 2096 4748 4958 1238
Size: 858 2652 210 3005
Fragments arranged by size:
3005 2652 858 210
SbfI CC_TGCA'GG
Cuts at: 3772 3772
Size: 6725
ScaI AGT'ACT
Cuts at: 1512 6242 1512
Size: 4730 1995
SfcI C'TryA_G
Cuts at: 33 638 686 1342 2203 3768 4453 5421 5612
Size: 605 48 656 861 1565 685 968 191
Cuts at: 5612 33
Size: 1146
Fragments arranged by size:
1565 1146 968 861 685 656 605 191
48
SfoI GGC'GCC
Cuts at: 4157 4405 4157
Size: 248 6477
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6725
SmaI CCC'GGG
Cuts at: 3747 3747
Size: 6725
SmlI C'TyrA_G
Cuts at: 892 1647 2195 3259 3880 4795 5509 5771 892
Size: 755 548 1064 621 915 714 262 1846
Fragments arranged by size:
1846 1064 915 755 714 621 548 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6725
SphI G_CATG'C
Cuts at: 3778 4278 3778
Size: 500 6225
SspI AAT'ATT
Cuts at: 1986 1986
Size: 6725
StuI AGG'CCT
Cuts at: 935 2817 935
Size: 1882 4843
StyI C'CwwG_G
Cuts at: 936 1992 3859 4213 936
Size: 1056 1867 354 3448
Fragments arranged by size:
3448 1867 1056 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4308 4441 4900 5109 4308
Size: 133 459 209 5924
Fragments arranged by size:
5924 459 209 133
TatI w'GTAC_w
Cuts at: 142 645 1280 1438 1510 1562 1901 3119 3654
Size: 503 635 158 72 52 339 1218 535
Cuts at: 3654 6240 142
Size: 2586 627
Fragments arranged by size:
2586 1218 635 627 535 503 339 158
72 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3012 3044 6242 6274 3012
Size: 32 3198 32 3463
Fragments arranged by size:
3463 3198 32 32
Tth111I GACn'n_nGTC
Cuts at: 1600 4522 1600
Size: 2922 3803
XbaI T'CTAG_A
Cuts at: 3222 3756 3222
Size: 534 6191
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1999 4220 1999
Size: 2221 4504
XmaI C'CCGG_G
Cuts at: 3745 3745
Size: 6725
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PpuMI
PsiI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BmtI BsiWI BssHII BstEII ClaI CspCI
FseI FspAI NcoI NheI NotI NruI PacI PasI
PfoI PmlI PshAI PspOMI PspXI SacII SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI