Restriction map for
pF1KA1292
(Circular) (Six-base) MAPSORT of: pF1KA1292.seq Check: 9540 from: 1 to: 5219
pF1KA1292 5219 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 567 981 1929 567
Size: 414 948 3857
Fragments arranged by size:
3857 948 414
AatII G_ACGT'C
Cuts at: 1798 1843 1798
Size: 45 5174
AccI GT'mk_AC
Cuts at: 425 2257 425
Size: 1832 3387
Acc65I G'GTAC_C
Cuts at: 2235 2235
Size: 5219
AclI AA'CG_TT
Cuts at: 5059 5059
Size: 5219
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1476 2080 3044 3476 4223 4491 808
Size: 668 604 964 432 747 268 1536
Fragments arranged by size:
1536 964 747 668 604 432 268
AflIII A'CryG_T
Cuts at: 2571 3675 4652 2571
Size: 1104 977 3138
Fragments arranged by size:
3138 1104 977
AgeI A'CCGG_T
Cuts at: 3607 3607
Size: 5219
AhdI GACnn_n'nnGTC
Cuts at: 1101 1548 1101
Size: 447 4772
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 922 922
Size: 5219
AlwNI CAG_nnn'CTG
Cuts at: 284 1054 1269 1935 2524 4091 284
Size: 770 215 666 589 1567 1412
Fragments arranged by size:
1567 1412 770 666 589 215
ApaI G_GGCC'C
Cuts at: 1289 1510 1708 1760 1892 1981 2138 1289
Size: 221 198 52 132 89 157 4370
Fragments arranged by size:
4370 221 198 157 132 89 52
ApaLI G'TGCA_C
Cuts at: 389 1829 3989 389
Size: 1440 2160 1619
Fragments arranged by size:
2160 1619 1440
ApoI r'AATT_y
Cuts at: 2223 4572 4828 2223
Size: 2349 256 2614
Fragments arranged by size:
2614 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5219
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5219
AvaI C'yCGr_G
Cuts at: 140 554 617 1197 1230 1517 1709 1884 2094
Size: 414 63 580 33 287 192 175 210
Cuts at: 2094 2239 140
Size: 145 3120
Fragments arranged by size:
3120 580 414 287 210 192 175 145
63 33
BamHI G'GATC_C
Cuts at: 2244 2244
Size: 5219
BanI G'GyrC_C
Cuts at: 663 1017 1771 2235 2649 2897 2932 663
Size: 354 754 464 414 248 35 2950
Fragments arranged by size:
2950 754 464 414 354 248 35
BanII G_rGCy'C
Cuts at: 183 1289 1510 1526 1708 1760 1892 1981 2138
Size: 1106 221 16 182 52 132 89 157
Cuts at: 2138 2233 3263 183
Size: 95 1030 2139
Fragments arranged by size:
2139 1106 1030 221 182 157 132 95
89 52 16
BbeI G_GCGC'C
Cuts at: 667 1775 2653 2901 667
Size: 1108 878 248 2985
Fragments arranged by size:
2985 1108 878 248
BbsI GAAGACnn'nnnn_
Cuts at: 2068 2068
Size: 5219
BbvCI CC'TCA_GC
Cuts at: 443 2054 443
Size: 1611 3608
BciVI GTATCCnnnnn_n'
Cuts at: 223 1420 3117 3878 5219 223
Size: 1197 1697 761 1341 223
Fragments arranged by size:
1697 1341 1197 761 223
BclI T'GATC_A
Cuts at: 1863 2739 1863
Size: 876 4343
BglI GCCn_nnn'nGGC
Cuts at: 530 690 1241 530
Size: 160 551 4508
Fragments arranged by size:
4508 551 160
BglII A'GATC_T
Cuts at: 2204 2734 4771 2204
Size: 530 2037 2652
Fragments arranged by size:
2652 2037 530
BlpI GC'TnA_GC
Cuts at: 1177 2331 1177
Size: 1154 4065
Bme1580I G_kGCm'C
Cuts at: 393 942 1022 1289 1510 1708 1760 1833 1892
Size: 549 80 267 221 198 52 73 59
Cuts at: 1892 1981 2138 2844 2937 3993 393
Size: 89 157 706 93 1056 1619
Fragments arranged by size:
1619 1056 706 549 267 221 198 157
93 89 80 73 59 52
BmrI ACTGGGnnnn_n'
Cuts at: 593 2431 2512 2847 4991 593
Size: 1838 81 335 2144 821
Fragments arranged by size:
2144 1838 821 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3586 3586
Size: 5219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1719 1719
Size: 5219
Bpu10I CC'TnA_GC
Cuts at: 443 1216 2054 443
Size: 773 838 3608
Fragments arranged by size:
3608 838 773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 1207 2395 3274 3766 4064 745
Size: 462 1188 879 492 298 1900
Fragments arranged by size:
1900 1188 879 492 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 4923 4923
Size: 5219
BsaAI yAC'GTr
Cuts at: 242 429 645 675 2572 242
Size: 187 216 30 1897 2889
Fragments arranged by size:
2889 1897 216 187 30
BsaBI GATnn'nnATC
Cuts at: 6 2733 4770 6
Size: 2727 2037 455
Fragments arranged by size:
2727 2037 455
BsaHI Gr'CG_yC
Cuts at: 345 404 664 1649 1772 1795 1840 2650 2898
Size: 59 260 985 123 23 45 810 248
Cuts at: 2898 3600 5096 345
Size: 702 1496 468
Fragments arranged by size:
1496 985 810 702 468 260 248 123
59 45 23
BsaWI w'CCGG_w
Cuts at: 2408 2929 3607 3881 4028 4439 2408
Size: 521 678 274 147 411 3188
Fragments arranged by size:
3188 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1609 1839 1609
Size: 230 4989
BseYI C'CCAG_C
Cuts at: 450 1407 1503 1663 1753 1897 2109 2644 3402
Size: 957 96 160 90 144 212 535 758
Cuts at: 3402 3979 4475 450
Size: 577 496 1194
Fragments arranged by size:
1194 957 758 577 535 496 212 160
144 96 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 737 970 1907 2059 737
Size: 233 937 152 3897
Fragments arranged by size:
3897 937 233 152
BsiEI CG_ry'CG
Cuts at: 104 2761 2807 4015 104
Size: 2657 46 1208 1308
Fragments arranged by size:
2657 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 393 644 1833 2233 3011 3201 3993 393
Size: 251 1189 400 778 190 792 1619
Fragments arranged by size:
1619 1189 792 778 400 251 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1733 1733
Size: 5219
Bsp1286I G_dGCh'C
Cuts at: 183 393 644 942 1022 1289 1510 1526 1708
Size: 210 251 298 80 267 221 16 182
Cuts at: 1708 1760 1833 1892 1981 2138 2233 2844 2937
Size: 52 73 59 89 157 95 611 93
Cuts at: 2937 3011 3201 3263 3993 183
Size: 74 190 62 730 1409
Fragments arranged by size:
1409 730 611 298 267 251 221 210
190 182 157 95 93 89 80 74
73 62 59 52 16
BspEI T'CCGG_A
Cuts at: 4439 4439
Size: 5219
BspHI T'CATG_A
Cuts at: 4395 4395
Size: 5219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 567 914 981 1032 1451 1929 2124 2269 2483
Size: 347 67 51 419 478 195 145 214
Cuts at: 2483 2785 3166 567
Size: 302 381 2620
Fragments arranged by size:
2620 478 419 381 347 302 214 195
145 67 51
BsrBI CCG'CTC
Cuts at: 50 145 699 759 1176 3511 3565 5049 5214
Size: 95 554 60 417 2335 54 1484 165
Cuts at: 5214 50
Size: 55
Fragments arranged by size:
2335 1484 554 417 165 95 60 55
54
BsrDI GCAATG_nn'
Cuts at: 3131 3131
Size: 5219
BsrFI r'CCGG_y
Cuts at: 496 830 993 1235 2122 3217 3398 3607 496
Size: 334 163 242 887 1095 181 209 2108
Fragments arranged by size:
2108 1095 887 334 242 209 181 163
BsrGI T'GTAC_A
Cuts at: 188 980 188
Size: 792 4427
BssSI C'ACGA_G
Cuts at: 3490 3848 3490
Size: 358 4861
BstAPI GCAn_nnn'nTGC
Cuts at: 1269 2596 1269
Size: 1327 3892
BstBI TT'CG_AA
Cuts at: 3580 3580
Size: 5219
BstEII G'GTnAC_C
Cuts at: 1916 1916
Size: 5219
BstXI CCAn_nnnn'nTGG
Cuts at: 120 477 120
Size: 357 4862
BstYI r'GATC_y
Cuts at: 1 2204 2244 2711 2734 3069 3315 4327 4413
Size: 2203 40 467 23 335 246 1012 86
Cuts at: 4413 4425 4771 1
Size: 12 346 449
Fragments arranged by size:
2203 1012 467 449 346 335 246 86
40 23 12
Bsu36I CC'TnA_GG
Cuts at: 727 727
Size: 5219
BtgI C'CryG_G
Cuts at: 107 476 594 1004 1471 1834 107
Size: 369 118 410 467 363 3492
Fragments arranged by size:
3492 467 410 369 363 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3169 3350 3513 3169
Size: 181 163 4875
Fragments arranged by size:
4875 181 163
BtsI GCAGTG_nn'
Cuts at: 273 315 865 906 933 1324 1908 2462 273
Size: 42 550 41 27 391 584 554 3030
Fragments arranged by size:
3030 584 554 550 391 42 41 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 836 871 836
Size: 35 5184
DraI TTT'AAA
Cuts at: 2218 2218
Size: 5219
DraIII CAC_nnn'GTG
Cuts at: 388 399 388
Size: 11 5208
DrdI GACnn_nn'nnGTC
Cuts at: 1648 1800 2925 3783 1648
Size: 152 1125 858 3084
Fragments arranged by size:
3084 1125 858 152
EaeI y'GGCC_r
Cuts at: 110 494 1386 1421 2804 2978 3369 3396 4741
Size: 384 892 35 1383 174 391 27 1345
Cuts at: 4741 110
Size: 588
Fragments arranged by size:
1383 1345 892 588 391 384 174 35
27
EagI C'GGCC_G
Cuts at: 2804 2804
Size: 5219
EarI CTCTTCn'nnn_
Cuts at: 508 1639 3242 3452 508
Size: 1131 1603 210 2275
Fragments arranged by size:
2275 1603 1131 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3427 3737 3883 5029 3427
Size: 310 146 1146 3617
Fragments arranged by size:
3617 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2231 2231
Size: 5219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1476 1719 2080 3044 3476 4223 4491 808
Size: 668 243 361 964 432 747 268 1536
Fragments arranged by size:
1536 964 747 668 432 361 268 243
EcoNI CCTnn'n_nnAGG
Cuts at: 1681 1681
Size: 5219
EcoO109I rG'GnC_Cy
Cuts at: 1009 1051 1143 1285 1286 1507 1704 1705 1714
Size: 42 92 142 1 221 197 1 9
Cuts at: 1714 1888 1889 1932 1978 2113 2134 2135 2358
Size: 174 1 43 46 135 21 1 223
Cuts at: 2358 1009
Size: 3870
Fragments arranged by size:
3870 223 221 197 174 142 135 92
46 43 42 21 9 1 1 1
1
EcoRI G'AATT_C
Cuts at: 2223 2223
Size: 5219
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1038 3238 1038
Size: 2200 3019
FseI GG_CCGG'CC
Cuts at: 500 500
Size: 5219
FspI TGC'GCA
Cuts at: 3000 3000
Size: 5219
HaeII r_GCGC'y
Cuts at: 667 1184 1205 1608 1775 2653 2901 3923 4902
Size: 517 21 403 167 878 248 1022 979
Cuts at: 4902 667
Size: 984
Fragments arranged by size:
1022 984 979 878 517 403 248 167
21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1087 1119 1180 1212 1534 1566 3061 3091 3093
Size: 32 61 32 322 32 1495 30 2
Cuts at: 3093 3123 1087
Size: 30 3183
Fragments arranged by size:
3183 1495 322 61 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 330 2258 330
Size: 1928 3291
HindIII A'AGCT_T
Cuts at: 769 2077 769
Size: 1308 3911
KasI G'GCGC_C
Cuts at: 663 1771 2649 2897 663
Size: 1108 878 248 2985
Fragments arranged by size:
2985 1108 878 248
KpnI G_GTAC'C
Cuts at: 2239 2239
Size: 5219
MluI A'CGCG_T
Cuts at: 4652 4652
Size: 5219
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3890 4074 3890
Size: 184 5035
MscI TGG'CCA
Cuts at: 112 1388 2980 112
Size: 1276 1592 2351
Fragments arranged by size:
2351 1592 1276
MslI CAynn'nnrTG
Cuts at: 118 475 490 3208 3335 118
Size: 357 15 2718 127 2002
Fragments arranged by size:
2718 2002 357 127 15
MspA1I CmG'CkG
Cuts at: 186 596 1836 2058 2330 2644 3004 4017 4262
Size: 410 1240 222 272 314 360 1013 245
Cuts at: 4262 186
Size: 1143
Fragments arranged by size:
1240 1143 1013 410 360 314 272 245
222
NaeI GCC'GGC
Cuts at: 498 995 1237 3400 498
Size: 497 242 2163 2317
Fragments arranged by size:
2317 2163 497 242
NarI GG'CG_CC
Cuts at: 664 1772 2650 2898 664
Size: 1108 878 248 2985
Fragments arranged by size:
2985 1108 878 248
NcoI C'CATG_G
Cuts at: 107 476 107
Size: 369 4850
NgoMIV G'CCGG_C
Cuts at: 496 993 1235 3398 496
Size: 497 242 2163 2317
Fragments arranged by size:
2317 2163 497 242
NspI r_CATG'y
Cuts at: 1153 2272 2772 3679 1153
Size: 1119 500 907 2693
Fragments arranged by size:
2693 1119 907 500
PasI CC'CwG_GG
Cuts at: 323 1068 2131 323
Size: 745 1063 3411
Fragments arranged by size:
3411 1063 745
PciI A'CATG_T
Cuts at: 3675 3675
Size: 5219
PflMI CCAn_nnn'nTGG
Cuts at: 1414 1414
Size: 5219
PfoI T'CCnGG_A
Cuts at: 740 1552 1574 740
Size: 812 22 4385
Fragments arranged by size:
4385 812 22
PmeI GTTT'AAAC
Cuts at: 2218 2218
Size: 5219
PmlI CAC'GTG
Cuts at: 242 645 242
Size: 403 4816
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4358 4390 4358
Size: 32 5187
PpuMI rG'GwC_Cy
Cuts at: 1009 1714 2113 1009
Size: 705 399 4115
Fragments arranged by size:
4115 705 399
PspOMI G'GGCC_C
Cuts at: 1285 1506 1704 1756 1888 1977 2134 1285
Size: 221 198 52 132 89 157 4370
Fragments arranged by size:
4370 221 198 157 132 89 52
PspXI vC'TCGA_Gb
Cuts at: 1230 1230
Size: 5219
PstI C_TGCA'G
Cuts at: 280 911 1265 1682 1793 1859 2266 2951 280
Size: 631 354 417 111 66 407 685 2548
Fragments arranged by size:
2548 685 631 417 407 354 111 66
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5219
PvuII CAG'CTG
Cuts at: 2058 2644 3004 2058
Size: 586 360 4273
Fragments arranged by size:
4273 586 360
RsrII CG'GwC_CG
Cuts at: 3414 3414
Size: 5219
SacI G_AGCT'C
Cuts at: 2233 2233
Size: 5219
SacII CC_GC'GG
Cuts at: 597 1837 597
Size: 1240 3979
SalI G'TCGA_C
Cuts at: 2256 2256
Size: 5219
SanDI GG'GwC_CC
Cuts at: 1009 1009
Size: 5219
SapI GCTCTTCn'nnn_
Cuts at: 3242 3452 3242
Size: 210 5009
SbfI CC_TGCA'GG
Cuts at: 1859 2266 1859
Size: 407 4812
ScaI AGT'ACT
Cuts at: 4736 4736
Size: 5219
SfcI C'TryA_G
Cuts at: 33 276 907 1261 1671 1678 1789 1855 2262
Size: 243 631 354 410 7 111 66 407
Cuts at: 2262 2947 3940 4131 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 631 410 407 354 243
191 111 66 7
SfoI GGC'GCC
Cuts at: 665 1773 2651 2899 665
Size: 1108 878 248 2985
Fragments arranged by size:
2985 1108 878 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5219
SmaI CCC'GGG
Cuts at: 1519 1886 2096 2241 1519
Size: 367 210 145 4497
Fragments arranged by size:
4497 367 210 145
SmlI C'TyrA_G
Cuts at: 760 1222 1230 2374 3289 3781 4043 760
Size: 462 8 1144 915 492 262 1936
Fragments arranged by size:
1936 1144 915 492 462 262 8
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5219
SphI G_CATG'C
Cuts at: 1153 2272 2772 1153
Size: 1119 500 3600
Fragments arranged by size:
3600 1119 500
StuI AGG'CCT
Cuts at: 732 1228 732
Size: 496 4723
StyI C'CwwG_G
Cuts at: 107 476 815 1013 2353 2707 107
Size: 369 339 198 1340 354 2619
Fragments arranged by size:
2619 1340 369 354 339 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 761 1421 2176 2802 2935 3394 3603 761
Size: 660 755 626 133 459 209 2377
Fragments arranged by size:
2377 755 660 626 459 209 133
TatI w'GTAC_w
Cuts at: 188 980 4734 188
Size: 792 3754 673
Fragments arranged by size:
3754 792 673
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1951 1983 4736 4768 1951
Size: 32 2753 32 2402
Fragments arranged by size:
2753 2402 32 32
Tth111I GACn'n_nGTC
Cuts at: 1842 3016 1842
Size: 1174 4045
XbaI T'CTAG_A
Cuts at: 2250 2250
Size: 5219
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 474 822 1411 1560 2714 474
Size: 348 589 149 1154 2979
Fragments arranged by size:
2979 1154 589 348 149
XhoI C'TCGA_G
Cuts at: 1230 1230
Size: 5219
XmaI C'CCGG_G
Cuts at: 1517 1884 2094 2239 1517
Size: 367 210 145 4497
Fragments arranged by size:
4497 367 210 145
XmnI GAAnn'nnTTC
Cuts at: 610 610
Size: 5219
ZraI GAC'GTC
Cuts at: 1796 1841 1796
Size: 45 5174
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AleI AloI AscI AvrII BaeI BcgI
BfrBI BmgBI BmtI BsaXI BsiWI BsmI BssHII BstZ17I
ClaI EcoRV FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PshAI PsiI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI