Restriction map for
pF1KA1296
(Circular) (Six-base) MAPSORT of: pF1KA1296.seq Check: 6433 from: 1 to: 5456
pF1KA1296 5456 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AatII G_ACGT'C
Cuts at: 1548 1548
Size: 5456
AccI GT'mk_AC
Cuts at: 2494 2494
Size: 5456
Acc65I G'GTAC_C
Cuts at: 2472 2472
Size: 5456
AclI AA'CG_TT
Cuts at: 5296 5296
Size: 5456
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 1022 1141 1271 1411 1795 1995 3281 3713
Size: 609 119 130 140 384 200 1286 432
Cuts at: 3713 4066 4728 413
Size: 353 662 1141
Fragments arranged by size:
1286 1141 662 609 432 384 353 200
140 130 119
AfeI AGC'GCT
Cuts at: 1675 1675
Size: 5456
AflII C'TTAA_G
Cuts at: 855 855
Size: 5456
AflIII A'CryG_T
Cuts at: 2808 4608 4889 2808
Size: 1800 281 3375
Fragments arranged by size:
3375 1800 281
AleI CACnn'nnGTG
Cuts at: 2113 2113
Size: 5456
AlwNI CAG_nnn'CTG
Cuts at: 727 1434 1773 2761 4199 727
Size: 707 339 988 1438 1984
Fragments arranged by size:
1984 1438 988 707 339
ApaLI G'TGCA_C
Cuts at: 4294 4294
Size: 5456
ApoI r'AATT_y
Cuts at: 1280 1388 1531 1750 2460 4809 5065 1280
Size: 108 143 219 710 2349 256 1671
Fragments arranged by size:
2349 1671 710 256 219 143 108
AseI AT'TA_AT
Cuts at: 20 20
Size: 5456
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5456
AvaI C'yCGr_G
Cuts at: 2076 2476 2076
Size: 400 5056
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1982 2015 2428 2461 1982
Size: 33 413 33 4977
Fragments arranged by size:
4977 413 33 33
BamHI G'GATC_C
Cuts at: 285 2072 2481 285
Size: 1787 409 3260
Fragments arranged by size:
3260 1787 409
BanI G'GyrC_C
Cuts at: 156 2472 2886 3134 3169 156
Size: 2316 414 248 35 2443
Fragments arranged by size:
2443 2316 414 248 35
BanII G_rGCy'C
Cuts at: 376 1196 1258 1353 1445 2470 3500 376
Size: 820 62 95 92 1025 1030 2332
Fragments arranged by size:
2332 1030 1025 820 95 92 62
BbeI G_GCGC'C
Cuts at: 2890 3138 2890
Size: 248 5208
BbsI GAAGACnn'nnnn_
Cuts at: 247 329 1010 1220 1548 247
Size: 82 681 210 328 4155
Fragments arranged by size:
4155 681 328 210 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 670 704 1869 1903 670
Size: 34 1165 34 4223
Fragments arranged by size:
4223 1165 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3354 4410 5456 3354
Size: 1056 1046 3354
Fragments arranged by size:
3354 1056 1046
BclI T'GATC_A
Cuts at: 1827 2123 2976 1827
Size: 296 853 4307
Fragments arranged by size:
4307 853 296
BglI GCCn_nnn'nGGC
Cuts at: 1634 1634
Size: 5456
BglII A'GATC_T
Cuts at: 972 2971 5008 972
Size: 1999 2037 1420
Fragments arranged by size:
2037 1999 1420
BlpI GC'TnA_GC
Cuts at: 1257 2568 1257
Size: 1311 4145
Bme1580I G_kGCm'C
Cuts at: 3081 3174 4298 3081
Size: 93 1124 4239
Fragments arranged by size:
4239 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 314 1155 2668 2749 3084 5228 314
Size: 841 1513 81 335 2144 542
Fragments arranged by size:
2144 1513 841 542 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 774 3823 774
Size: 3049 2407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 433
Size: 5456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1429 2204 2632 3511 4225 4523 1429
Size: 775 428 879 714 298 2362
Fragments arranged by size:
2362 879 775 714 428 298
BsaI GGTCTCn'nnnn_
Cuts at: 1728 5160 1728
Size: 3432 2024
BsaAI yAC'GTr
Cuts at: 2115 2809 2115
Size: 694 4762
BsaBI GATnn'nnATC
Cuts at: 6 971 2970 5007 6
Size: 965 1999 2037 455
Fragments arranged by size:
2037 1999 965 455
BsaHI Gr'CG_yC
Cuts at: 1545 1626 1637 2887 3135 3837 5333 1545
Size: 81 11 1250 248 702 1496 1668
Fragments arranged by size:
1668 1496 1250 702 248 81 11
BsaWI w'CCGG_w
Cuts at: 893 1275 2645 3166 3844 4255 4402 4676 893
Size: 382 1370 521 678 411 147 274 1673
Fragments arranged by size:
1673 1370 678 521 411 382 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 999 1029 1082 1112 1790 1820 2330 2360 999
Size: 30 53 30 678 30 510 30 4095
Fragments arranged by size:
4095 678 510 53 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1678 2166 2169 1678
Size: 488 3 4965
Fragments arranged by size:
4965 488 3
BseYI C'CCAG_C
Cuts at: 479 1101 2225 2881 3639 4304 4712 479
Size: 622 1124 656 758 665 408 1223
Fragments arranged by size:
1223 1124 758 665 656 622 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 1208 1960 285
Size: 923 752 3781
Fragments arranged by size:
3781 923 752
BsiEI CG_ry'CG
Cuts at: 104 1860 2998 3044 4274 104
Size: 1756 1138 46 1230 1286
Fragments arranged by size:
1756 1286 1230 1138 46
BsiHKAI G_wGCw'C
Cuts at: 677 847 1258 2470 3248 3438 4298 677
Size: 170 411 1212 778 190 860 1835
Fragments arranged by size:
1835 1212 860 778 411 190 170
BsmBI CGTCTCn'nnnn_
Cuts at: 1575 1575
Size: 5456
Bsp1286I G_dGCh'C
Cuts at: 376 677 847 1196 1258 1353 1445 2470 3081
Size: 301 170 349 62 95 92 1025 611
Cuts at: 3081 3174 3248 3438 3500 4298 376
Size: 93 74 190 62 798 1534
Fragments arranged by size:
1534 1025 798 611 349 301 190 170
95 93 92 74 62 62
BspEI T'CCGG_A
Cuts at: 893 1275 893
Size: 382 5074
BspHI T'CATG_A
Cuts at: 1548 3888 1548
Size: 2340 3116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1746 1932 2034 2174 2506 2720 3022 3403 1746
Size: 186 102 140 332 214 302 381 3799
Fragments arranged by size:
3799 381 332 302 214 186 140 102
BsrBI CCG'CTC
Cuts at: 50 316 1109 3748 3802 5286 5451 50
Size: 266 793 2639 54 1484 165 55
Fragments arranged by size:
2639 1484 793 266 165 55 54
BsrDI GCAATG_nn'
Cuts at: 174 3368 174
Size: 3194 2262
BsrFI r'CCGG_y
Cuts at: 3454 3635 3454
Size: 181 5275
BssSI C'ACGA_G
Cuts at: 3727 4435 3727
Size: 708 4748
BstAPI GCAn_nnn'nTGC
Cuts at: 2833 2833
Size: 5456
BstBI TT'CG_AA
Cuts at: 1539 3817 1539
Size: 2278 3178
BstYI r'GATC_y
Cuts at: 1 285 723 941 972 1359 2072 2481 2948
Size: 284 438 218 31 387 713 409 467
Cuts at: 2948 2971 3306 3552 3858 3870 3956 5008 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 713 467 449 438 409 387 335
306 284 246 218 86 31 23 12
Bsu36I CC'TnA_GG
Cuts at: 258 377 258
Size: 119 5337
BtgI C'CryG_G
Cuts at: 869 1523 1854 869
Size: 654 331 4471
Fragments arranged by size:
4471 654 331
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 875 2348 3406 3587 3750 875
Size: 1473 1058 181 163 2581
Fragments arranged by size:
2581 1473 1058 181 163
BtsI GCAGTG_nn'
Cuts at: 1334 2032 2198 2199 2699 1334
Size: 698 166 1 500 4091
Fragments arranged by size:
4091 698 500 166 1
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 636 671 636
Size: 35 5421
DraI TTT'AAA
Cuts at: 1761 2455 1761
Size: 694 4762
DrdI GACnn_nn'nnGTC
Cuts at: 3162 4506 3162
Size: 1344 4112
EaeI y'GGCC_r
Cuts at: 1085 1857 3041 3215 3606 3633 4978 1085
Size: 772 1184 174 391 27 1345 1563
Fragments arranged by size:
1563 1345 1184 772 391 174 27
EagI C'GGCC_G
Cuts at: 1857 3041 1857
Size: 1184 4272
EarI CTCTTCn'nnn_
Cuts at: 492 735 835 1116 1327 1430 1598 2229 3479
Size: 243 100 281 211 103 168 631 1250
Cuts at: 3479 3689 492
Size: 210 2259
Fragments arranged by size:
2259 1250 631 281 243 211 210 168
103 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 624 3664 4406 4552 5266 624
Size: 3040 742 146 714 814
Fragments arranged by size:
3040 814 742 714 146
EcoICRI GAG'CTC
Cuts at: 1256 2468 1256
Size: 1212 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 433 1022 1141 1271 1411 1795 1995 3281
Size: 20 589 119 130 140 384 200 1286
Cuts at: 3281 3713 4066 4728 413
Size: 432 353 662 1141
Fragments arranged by size:
1286 1141 662 589 432 384 353 200
140 130 119 20
EcoO109I rG'GnC_Cy
Cuts at: 1431 1683 2595 1431
Size: 252 912 4292
Fragments arranged by size:
4292 912 252
EcoRI G'AATT_C
Cuts at: 2460 2460
Size: 5456
EcoRV GAT'ATC
Cuts at: 2343 2343
Size: 5456
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1582 1995 3475 1582
Size: 413 1480 3563
Fragments arranged by size:
3563 1480 413
FspI TGC'GCA
Cuts at: 3237 3237
Size: 5456
HaeII r_GCGC'y
Cuts at: 1677 2890 3138 4368 5139 1677
Size: 1213 248 1230 771 1994
Fragments arranged by size:
1994 1230 1213 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 645 677 715 747 964 996 997 1029 1468
Size: 32 38 32 217 32 1 32 439
Cuts at: 1468 1500 1638 1670 2081 2113 3298 3328 3330
Size: 32 138 32 411 32 1185 30 2
Cuts at: 3330 3360 645
Size: 30 2741
Fragments arranged by size:
2741 1185 439 411 217 138 38 32
32 32 32 32 32 32 30 30
2 1
HincII GTy'rAC
Cuts at: 1931 2495 1931
Size: 564 4892
KasI G'GCGC_C
Cuts at: 2886 3134 2886
Size: 248 5208
KpnI G_GTAC'C
Cuts at: 2476 2476
Size: 5456
MluI A'CGCG_T
Cuts at: 4889 4889
Size: 5456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1340 1669 2303 4215 4399 1340
Size: 329 634 1912 184 2397
Fragments arranged by size:
2397 1912 634 329 184
MscI TGG'CCA
Cuts at: 3217 3217
Size: 5456
MslI CAynn'nnrTG
Cuts at: 235 2113 3445 3572 235
Size: 1878 1332 127 2119
Fragments arranged by size:
2119 1878 1332 127
MspA1I CmG'CkG
Cuts at: 1507 2567 2881 3241 4025 4270 1507
Size: 1060 314 360 784 245 2693
Fragments arranged by size:
2693 1060 784 360 314 245
NaeI GCC'GGC
Cuts at: 3637 3637
Size: 5456
NarI GG'CG_CC
Cuts at: 2887 3135 2887
Size: 248 5208
NcoI C'CATG_G
Cuts at: 869 869
Size: 5456
NgoMIV G'CCGG_C
Cuts at: 3635 3635
Size: 5456
NspI r_CATG'y
Cuts at: 2509 3009 4612 2509
Size: 500 1603 3353
Fragments arranged by size:
3353 1603 500
PciI A'CATG_T
Cuts at: 4608 4608
Size: 5456
PfoI T'CCnGG_A
Cuts at: 1296 1296
Size: 5456
PmeI GTTT'AAAC
Cuts at: 2455 2455
Size: 5456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 3902
Size: 32 5424
PpuMI rG'GwC_Cy
Cuts at: 1683 1683
Size: 5456
PshAI GACnn'nnGTC
Cuts at: 2252 2252
Size: 5456
PsiI TTA'TAA
Cuts at: 1394 1817 1394
Size: 423 5033
PstI C_TGCA'G
Cuts at: 1793 2503 3188 1793
Size: 710 685 4061
Fragments arranged by size:
4061 710 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5456
PvuII CAG'CTG
Cuts at: 1507 2881 3241 1507
Size: 1374 360 3722
Fragments arranged by size:
3722 1374 360
RsrII CG'GwC_CG
Cuts at: 3651 3651
Size: 5456
SacI G_AGCT'C
Cuts at: 1258 2470 1258
Size: 1212 4244
SalI G'TCGA_C
Cuts at: 2493 2493
Size: 5456
SapI GCTCTTCn'nnn_
Cuts at: 835 1116 3479 3689 835
Size: 281 2363 210 2602
Fragments arranged by size:
2602 2363 281 210
SbfI CC_TGCA'GG
Cuts at: 2503 2503
Size: 5456
ScaI AGT'ACT
Cuts at: 4973 4973
Size: 5456
SexAI A'CCwGG_T
Cuts at: 405 405
Size: 5456
SfcI C'TryA_G
Cuts at: 33 275 342 507 1789 2499 3184 4152 4343
Size: 242 67 165 1282 710 685 968 191
Cuts at: 4343 33
Size: 1146
Fragments arranged by size:
1282 1146 968 710 685 242 191 165
67
SfoI GGC'GCC
Cuts at: 2888 3136 2888
Size: 248 5208
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5456
SmaI CCC'GGG
Cuts at: 2078 2478 2078
Size: 400 5056
SmlI C'TyrA_G
Cuts at: 855 1444 2219 2611 3526 4240 4502 855
Size: 589 775 392 915 714 262 1809
Fragments arranged by size:
1809 915 775 714 589 392 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5456
SphI G_CATG'C
Cuts at: 2509 3009 2509
Size: 500 4956
StuI AGG'CCT
Cuts at: 263 263
Size: 5456
StyI C'CwwG_G
Cuts at: 500 869 2590 2944 500
Size: 369 1721 354 3012
Fragments arranged by size:
3012 1721 369 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3039 3172 3631 3840 3039
Size: 133 459 209 4655
Fragments arranged by size:
4655 459 209 133
TatI w'GTAC_w
Cuts at: 977 4971 977
Size: 3994 1462
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 999 1031 1082 1114 4973 5005 999
Size: 32 51 32 3859 32 1450
Fragments arranged by size:
3859 1450 51 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3253 3253
Size: 5456
XbaI T'CTAG_A
Cuts at: 2487 2487
Size: 5456
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 876 2112 2951 876
Size: 1236 839 3381
Fragments arranged by size:
3381 1236 839
XmaI C'CCGG_G
Cuts at: 2076 2476 2076
Size: 400 5056
XmnI GAAnn'nnTTC
Cuts at: 597 1535 597
Size: 938 4518
ZraI GAC'GTC
Cuts at: 1546 1546
Size: 5456
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AgeI AhdI AlfI AloI ApaI AscI AvrII
BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I ClaI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI