Restriction map for
pF1KA1313
(Circular) (Six-base) MAPSORT of: pF1KA1313.seq Check: 3039 from: 1 to: 6416
pF1KA1313 6416 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1584 1584
Size: 6416
AatII G_ACGT'C
Cuts at: 1775 1775
Size: 6416
AccI GT'mk_AC
Cuts at: 929 1832 3454 929
Size: 903 1622 3891
Fragments arranged by size:
3891 1622 903
Acc65I G'GTAC_C
Cuts at: 2331 3432 2331
Size: 1101 5315
AclI AA'CG_TT
Cuts at: 2366 6256 2366
Size: 3890 2526
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 2698 3314 3373 3415 4241 4673 5026 5688
Size: 2432 616 59 42 826 432 353 662
Cuts at: 5688 266
Size: 994
Fragments arranged by size:
2432 994 826 662 616 432 353 59
42
AfeI AGC'GCT
Cuts at: 564 564
Size: 6416
AflIII A'CryG_T
Cuts at: 1037 1254 3768 5568 5849 1037
Size: 217 2514 1800 281 1604
Fragments arranged by size:
2514 1800 1604 281 217
AleI CACnn'nnGTG
Cuts at: 2779 2779
Size: 6416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2229 2229
Size: 6416
AlwNI CAG_nnn'CTG
Cuts at: 2427 2876 3721 5159 2427
Size: 449 845 1438 3684
Fragments arranged by size:
3684 1438 845 449
ApaI G_GGCC'C
Cuts at: 1076 2540 1076
Size: 1464 4952
ApaLI G'TGCA_C
Cuts at: 5254 5254
Size: 6416
ApoI r'AATT_y
Cuts at: 1696 3420 5769 6025 1696
Size: 1724 2349 256 2087
Fragments arranged by size:
2349 2087 1724 256
AseI AT'TA_AT
Cuts at: 20 176 20
Size: 156 6260
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6416
AvaI C'yCGr_G
Cuts at: 415 1197 2336 3436 415
Size: 782 1139 1100 3395
Fragments arranged by size:
3395 1139 1100 782
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2741 2774 2741
Size: 33 6383
BamHI G'GATC_C
Cuts at: 3441 3441
Size: 6416
BanI G'GyrC_C
Cuts at: 766 910 1513 2331 3432 3846 4094 4129 766
Size: 144 603 818 1101 414 248 35 3053
Fragments arranged by size:
3053 1101 818 603 414 248 144 35
BanII G_rGCy'C
Cuts at: 397 611 971 1076 2008 2128 2167 2540 2628
Size: 214 360 105 932 120 39 373 88
Cuts at: 2628 3187 3305 3430 4460 397
Size: 559 118 125 1030 2353
Fragments arranged by size:
2353 1030 932 559 373 360 214 125
120 118 105 88 39
BbeI G_GCGC'C
Cuts at: 1517 3850 4098 1517
Size: 2333 248 3835
Fragments arranged by size:
3835 2333 248
BbsI GAAGACnn'nnnn_
Cuts at: 1605 1892 1605
Size: 287 6129
BbvCI CC'TCA_GC
Cuts at: 3047 3047
Size: 6416
BciVI GTATCCnnnnn_n'
Cuts at: 4314 5370 6416 4314
Size: 1056 1046 4314
Fragments arranged by size:
4314 1056 1046
BclI T'GATC_A
Cuts at: 2808 2814 3936 2808
Size: 6 1122 5288
Fragments arranged by size:
5288 1122 6
BfrBI ATG'CAT
Cuts at: 3333 3333
Size: 6416
BglI GCCn_nnn'nGGC
Cuts at: 232 232
Size: 6416
BglII A'GATC_T
Cuts at: 518 3931 5968 518
Size: 3413 2037 966
Fragments arranged by size:
3413 2037 966
BlpI GC'TnA_GC
Cuts at: 3299 3528 3299
Size: 229 6187
Bme1580I G_kGCm'C
Cuts at: 769 913 1037 1076 1286 1400 2540 4041 4134
Size: 144 124 39 210 114 1140 1501 93
Cuts at: 4134 5258 769
Size: 1124 1927
Fragments arranged by size:
1927 1501 1140 1124 210 144 124 114
93 39
BmgBI CAC'GTC
Cuts at: 1053 1255 1053
Size: 202 6214
BmrI ACTGGGnnnn_n'
Cuts at: 482 3628 3709 4044 6188 482
Size: 3146 81 335 2144 710
Fragments arranged by size:
3146 2144 710 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1329 4783 1329
Size: 3454 2962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 655 3268 535
Size: 120 2613 3683
Fragments arranged by size:
3683 2613 120
Bpu10I CC'TnA_GC
Cuts at: 1061 2918 3047 1061
Size: 1857 129 4430
Fragments arranged by size:
4430 1857 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 3163 3592 4471 5185 5483 166
Size: 2997 429 879 714 298 1099
Fragments arranged by size:
2997 1099 879 714 429 298
BsaI GGTCTCn'nnnn_
Cuts at: 1561 6120 1561
Size: 4559 1857
BsaAI yAC'GTr
Cuts at: 1038 1206 2613 3769 1038
Size: 168 1407 1156 3685
Fragments arranged by size:
3685 1407 1156 168
BsaBI GATnn'nnATC
Cuts at: 6 2813 3930 5967 6
Size: 2807 1117 2037 455
Fragments arranged by size:
2807 2037 1117 455
BsaHI Gr'CG_yC
Cuts at: 1514 1772 3847 4095 4797 6293 1514
Size: 258 2075 248 702 1496 1637
Fragments arranged by size:
2075 1637 1496 702 258 248
BsaWI w'CCGG_w
Cuts at: 2237 3605 4126 4804 5215 5362 5636 2237
Size: 1368 521 678 411 147 274 3017
Fragments arranged by size:
3017 1368 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 2362 2382 2710 3064 214
Size: 2148 20 328 354 3566
Fragments arranged by size:
3566 2148 354 328 20
BseYI C'CCAG_C
Cuts at: 334 1272 2540 3234 3841 4599 5264 5672 334
Size: 938 1268 694 607 758 665 408 1078
Fragments arranged by size:
1268 1078 938 758 694 665 607 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1075 1279 1513 616
Size: 459 204 234 5519
Fragments arranged by size:
5519 459 234 204
BsiEI CG_ry'CG
Cuts at: 104 957 1941 3958 4004 5234 104
Size: 853 984 2017 46 1230 1286
Fragments arranged by size:
2017 1286 1230 984 853 46
BsiHKAI G_wGCw'C
Cuts at: 611 971 1433 2008 2458 3187 3430 4208 4398
Size: 360 462 575 450 729 243 778 190
Cuts at: 4398 5258 611
Size: 860 1769
Fragments arranged by size:
1769 860 778 729 575 462 450 360
243 190
BsmI GAATG_Cn'
Cuts at: 2851 2851
Size: 6416
BsmBI CGTCTCn'nnnn_
Cuts at: 697 697
Size: 6416
Bsp1286I G_dGCh'C
Cuts at: 397 611 769 913 971 1037 1076 1286 1400
Size: 214 158 144 58 66 39 210 114
Cuts at: 1400 1433 2008 2128 2167 2458 2540 2628 3187
Size: 33 575 120 39 291 82 88 559
Cuts at: 3187 3305 3430 4041 4134 4208 4398 4460 5258
Size: 118 125 611 93 74 190 62 798
Cuts at: 5258 397
Size: 1555
Fragments arranged by size:
1555 798 611 575 559 291 214 210
190 158 144 125 120 118 114 93
88 82 74 66 62 58 39 39
33
BspEI T'CCGG_A
Cuts at: 2237 2237
Size: 6416
BspHI T'CATG_A
Cuts at: 2811 4848 2811
Size: 2037 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1157 1584 3466 3680 3982 4363 1157
Size: 427 1882 214 302 381 3210
Fragments arranged by size:
3210 1882 427 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2447 4708 4762 6246 6411 50
Size: 2397 2261 54 1484 165 55
Fragments arranged by size:
2397 2261 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1284 2168 2832 3141 3218 4328 1284
Size: 884 664 309 77 1110 3372
Fragments arranged by size:
3372 1110 884 664 309 77
BsrFI r'CCGG_y
Cuts at: 126 1137 4414 4595 126
Size: 1011 3277 181 1947
Fragments arranged by size:
3277 1947 1011 181
BssHII G'CGCG_C
Cuts at: 207 207
Size: 6416
BssSI C'ACGA_G
Cuts at: 676 1675 4687 5395 676
Size: 999 3012 708 1697
Fragments arranged by size:
3012 1697 999 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2427 3793 2427
Size: 1366 5050
BstBI TT'CG_AA
Cuts at: 1694 4777 1694
Size: 3083 3333
BstEII G'GTnAC_C
Cuts at: 351 789 1101 351
Size: 438 312 5666
Fragments arranged by size:
5666 438 312
BstXI CCAn_nnnn'nTGG
Cuts at: 435 1323 1969 3225 435
Size: 888 646 1256 3626
Fragments arranged by size:
3626 1256 888 646
BstYI r'GATC_y
Cuts at: 1 518 2234 2794 3441 3908 3931 4266 4512
Size: 517 1716 560 647 467 23 335 246
Cuts at: 4512 4818 4830 4916 5968 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1716 1052 647 560 517 467 449 335
306 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1941 2046 2789 3306 107
Size: 1834 105 743 517 3217
Fragments arranged by size:
3217 1834 743 517 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2922 4366 4547 4710 2922
Size: 1444 181 163 4628
Fragments arranged by size:
4628 1444 181 163
BtsI GCAGTG_nn'
Cuts at: 731 1092 1267 1572 1732 1820 3659 731
Size: 361 175 305 160 88 1839 3488
Fragments arranged by size:
3488 1839 361 305 175 160 88
DraI TTT'AAA
Cuts at: 3415 3415
Size: 6416
DraIII CAC_nnn'GTG
Cuts at: 321 321
Size: 6416
DrdI GACnn_nn'nnGTC
Cuts at: 1609 4122 5466 1609
Size: 2513 1344 2559
Fragments arranged by size:
2559 2513 1344
EaeI y'GGCC_r
Cuts at: 143 233 918 1709 1908 2859 4001 4175 4566
Size: 90 685 791 199 951 1142 174 391
Cuts at: 4566 4593 5938 143
Size: 27 1345 621
Fragments arranged by size:
1345 1142 951 791 685 621 391 199
174 90 27
EagI C'GGCC_G
Cuts at: 4001 4001
Size: 6416
EarI CTCTTCn'nnn_
Cuts at: 191 1022 2385 4439 4649 191
Size: 831 1363 2054 210 1958
Fragments arranged by size:
2054 1958 1363 831 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 871 1124 2314 4624 5366 5512 6226 871
Size: 253 1190 2310 742 146 714 1061
Fragments arranged by size:
2310 1190 1061 742 714 253 146
EcoICRI GAG'CTC
Cuts at: 609 969 2006 3185 3428 609
Size: 360 1037 1179 243 3597
Fragments arranged by size:
3597 1179 1037 360 243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 535 655 2698 3268 3314 3373 3415 4241
Size: 269 120 2043 570 46 59 42 826
Cuts at: 4241 4673 5026 5688 266
Size: 432 353 662 994
Fragments arranged by size:
2043 994 826 662 570 432 353 269
120 59 46 42
EcoO109I rG'GnC_Cy
Cuts at: 468 1072 2536 2537 3555 468
Size: 604 1464 1 1018 3329
Fragments arranged by size:
3329 1464 1018 604 1
EcoRI G'AATT_C
Cuts at: 1696 3420 1696
Size: 1724 4692
EcoRV GAT'ATC
Cuts at: 3402 3402
Size: 6416
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4435 4435
Size: 6416
FspI TGC'GCA
Cuts at: 4197 4197
Size: 6416
HaeII r_GCGC'y
Cuts at: 566 1016 1193 1517 3850 4098 5328 6099 566
Size: 450 177 324 2333 248 1230 771 883
Fragments arranged by size:
2333 1230 883 771 450 324 248 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 409 441 562 594 694 726 1222 1254 2793
Size: 32 121 32 100 32 496 32 1539
Cuts at: 2793 2825 4258 4288 4290 4320 409
Size: 32 1433 30 2 30 2505
Fragments arranged by size:
2505 1539 1433 496 121 100 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 951 1161 1776 2760 3111 3261 3270 3455 951
Size: 210 615 984 351 150 9 185 3912
Fragments arranged by size:
3912 984 615 351 210 185 150 9
HindIII A'AGCT_T
Cuts at: 585 585
Size: 6416
KasI G'GCGC_C
Cuts at: 1513 3846 4094 1513
Size: 2333 248 3835
Fragments arranged by size:
3835 2333 248
KpnI G_GTAC'C
Cuts at: 2335 3436 2335
Size: 1101 5315
MluI A'CGCG_T
Cuts at: 5849 5849
Size: 6416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 326 2630 5175 5359 326
Size: 2304 2545 184 1383
Fragments arranged by size:
2545 2304 1383 184
MscI TGG'CCA
Cuts at: 145 235 1711 2861 4177 145
Size: 90 1476 1150 1316 2384
Fragments arranged by size:
2384 1476 1316 1150 90
MslI CAynn'nnrTG
Cuts at: 2779 4405 4532 2779
Size: 1626 127 4663
Fragments arranged by size:
4663 1626 127
MspA1I CmG'CkG
Cuts at: 555 1943 2733 3527 3841 4201 4985 5230 555
Size: 1388 790 794 314 360 784 245 1741
Fragments arranged by size:
1741 1388 794 790 784 360 314 245
NaeI GCC'GGC
Cuts at: 4597 4597
Size: 6416
NarI GG'CG_CC
Cuts at: 1514 3847 4095 1514
Size: 2333 248 3835
Fragments arranged by size:
3835 2333 248
NcoI C'CATG_G
Cuts at: 107 2789 107
Size: 2682 3734
NgoMIV G'CCGG_C
Cuts at: 4595 4595
Size: 6416
NruI TCG'CGA
Cuts at: 1235 1541 1235
Size: 306 6110
NsiI A_TGCA'T
Cuts at: 3335 3335
Size: 6416
NspI r_CATG'y
Cuts at: 1608 2926 3469 3969 5572 1608
Size: 1318 543 500 1603 2452
Fragments arranged by size:
2452 1603 1318 543 500
PasI CC'CwG_GG
Cuts at: 2160 2433 2160
Size: 273 6143
PciI A'CATG_T
Cuts at: 5568 5568
Size: 6416
PflMI CCAn_nnn'nTGG
Cuts at: 152 152
Size: 6416
PfoI T'CCnGG_A
Cuts at: 3122 3122
Size: 6416
PmeI GTTT'AAAC
Cuts at: 3415 3415
Size: 6416
PmlI CAC'GTG
Cuts at: 1038 1038
Size: 6416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4862 4894 4862
Size: 32 6384
PpuMI rG'GwC_Cy
Cuts at: 468 468
Size: 6416
PshAI GACnn'nnGTC
Cuts at: 1553 1873 1553
Size: 320 6096
PspOMI G'GGCC_C
Cuts at: 1072 2536 1072
Size: 1464 4952
PspXI vC'TCGA_Gb
Cuts at: 415 415
Size: 6416
PstI C_TGCA'G
Cuts at: 1298 1409 2254 3100 3463 4148 1298
Size: 111 845 846 363 685 3566
Fragments arranged by size:
3566 846 845 685 363 111
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6416
PvuII CAG'CTG
Cuts at: 3841 4201 3841
Size: 360 6056
RsrII CG'GwC_CG
Cuts at: 4611 4611
Size: 6416
SacI G_AGCT'C
Cuts at: 611 971 2008 3187 3430 611
Size: 360 1037 1179 243 3597
Fragments arranged by size:
3597 1179 1037 360 243
SacII CC_GC'GG
Cuts at: 1944 1944
Size: 6416
SalI G'TCGA_C
Cuts at: 3453 3453
Size: 6416
SapI GCTCTTCn'nnn_
Cuts at: 2385 4439 4649 2385
Size: 2054 210 4152
Fragments arranged by size:
4152 2054 210
SbfI CC_TGCA'GG
Cuts at: 3463 3463
Size: 6416
ScaI AGT'ACT
Cuts at: 187 2266 5933 187
Size: 2079 3667 670
Fragments arranged by size:
3667 2079 670
SexAI A'CCwGG_T
Cuts at: 1481 1862 1961 1481
Size: 381 99 5936
Fragments arranged by size:
5936 381 99
SfcI C'TryA_G
Cuts at: 33 717 1294 1405 2250 3096 3459 4144 5112
Size: 684 577 111 845 846 363 685 968
Cuts at: 5112 5303 33
Size: 191 1146
Fragments arranged by size:
1146 968 846 845 685 684 577 363
191 111
SfoI GGC'GCC
Cuts at: 1515 3848 4096 1515
Size: 2333 248 3835
Fragments arranged by size:
3835 2333 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6416
SmaI CCC'GGG
Cuts at: 1199 2338 3438 1199
Size: 1139 1100 4177
Fragments arranged by size:
4177 1139 1100
SmlI C'TyrA_G
Cuts at: 181 415 3178 3571 4486 5200 5462 181
Size: 234 2763 393 915 714 262 1135
Fragments arranged by size:
2763 1135 915 714 393 262 234
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6416
SphI G_CATG'C
Cuts at: 3469 3969 3469
Size: 500 5916
StuI AGG'CCT
Cuts at: 1244 3057 1244
Size: 1813 4603
StyI C'CwwG_G
Cuts at: 107 1214 1685 1712 2789 3104 3550 3904 107
Size: 1107 471 27 1077 315 446 354 2619
Fragments arranged by size:
2619 1107 1077 471 446 354 315 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 808 1945 3999 4132 4591 4800 808
Size: 1137 2054 133 459 209 2424
Fragments arranged by size:
2424 2054 1137 459 209 133
TatI w'GTAC_w
Cuts at: 185 2264 5931 185
Size: 2079 3667 670
Fragments arranged by size:
3667 2079 670
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 863 895 5933 5965 863
Size: 32 5038 32 1314
Fragments arranged by size:
5038 1314 32 32
Tth111I GACn'n_nGTC
Cuts at: 708 1963 4213 708
Size: 1255 2250 2911
Fragments arranged by size:
2911 2250 1255
XbaI T'CTAG_A
Cuts at: 3447 3447
Size: 6416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 921 1323 3911 921
Size: 402 2588 3426
Fragments arranged by size:
3426 2588 402
XhoI C'TCGA_G
Cuts at: 415 415
Size: 6416
XmaI C'CCGG_G
Cuts at: 1197 2336 3436 1197
Size: 1139 1100 4177
Fragments arranged by size:
4177 1139 1100
XmnI GAAnn'nnTTC
Cuts at: 2828 2828
Size: 6416
ZraI GAC'GTC
Cuts at: 1773 1773
Size: 6416
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AloI AscI AvrII BcgI BmtI
BsaXI BsiWI BsrGI BstZ17I Bsu36I ClaI CspCI EcoNI
FseI FspAI HpaI MfeI NdeI NheI NotI PacI
PsiI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI