(Circular) (Six-base) MAPSORT of: pF1KA1315.seq Check: 9361 from: 1 to: 9056 pF1KA1315 9056 bp With 182 enzymes: SgfI * June 20, 2008 10:21 .. AccI GT'mk_AC Cuts at: 1112 4342 6094 1112 Size: 3230 1752 4074 Fragments arranged by size: 4074 3230 1752 Acc65I G'GTAC_C Cuts at: 6072 6072 Size: 9056 AclI AA'CG_TT Cuts at: 1996 8896 1996 Size: 6900 2156 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 317 1535 1718 2357 2539 4265 4477 4535 4991 Size: 1218 183 639 182 1726 212 58 456 Cuts at: 4991 5741 6881 7313 7666 8328 317 Size: 750 1140 432 353 662 1045 Fragments arranged by size: 1726 1218 1140 1045 750 662 639 456 432 353 212 183 182 58 AfeI AGC'GCT Cuts at: 237 3862 237 Size: 3625 5431 AflII C'TTAA_G Cuts at: 172 439 903 3427 3544 4153 172 Size: 267 464 2524 117 609 5075 Fragments arranged by size: 5075 2524 609 464 267 117 AflIII A'CryG_T Cuts at: 2878 3066 3227 3249 6408 8208 8489 2878 Size: 188 161 22 3159 1800 281 3445 Fragments arranged by size: 3445 3159 1800 281 188 161 22 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 706 706 Size: 9056 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3835 3867 3835 Size: 32 9024 AlwNI CAG_nnn'CTG Cuts at: 1039 2032 2239 5182 6361 7799 1039 Size: 993 207 2943 1179 1438 2296 Fragments arranged by size: 2943 2296 1438 1179 993 207 ApaLI G'TGCA_C Cuts at: 7894 7894 Size: 9056 ApoI r'AATT_y Cuts at: 408 2461 2784 2886 2967 3271 3283 3813 4990 Size: 2053 323 102 81 304 12 530 1177 Cuts at: 4990 5203 5912 6060 8409 8665 408 Size: 213 709 148 2349 256 799 Fragments arranged by size: 2349 2053 1177 799 709 530 323 304 256 213 148 102 81 12 AseI AT'TA_AT Cuts at: 20 2216 3292 20 Size: 2196 1076 5784 Fragments arranged by size: 5784 2196 1076 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 9056 AvaI C'yCGr_G Cuts at: 6076 6076 Size: 9056 BamHI G'GATC_C Cuts at: 6081 6081 Size: 9056 BanI G'GyrC_C Cuts at: 6072 6486 6734 6769 6072 Size: 414 248 35 8359 Fragments arranged by size: 8359 414 248 35 BanII G_rGCy'C Cuts at: 2126 4957 6070 7100 2126 Size: 2831 1113 1030 4082 Fragments arranged by size: 4082 2831 1113 1030 BbeI G_GCGC'C Cuts at: 6490 6738 6490 Size: 248 8808 BbsI GAAGACnn'nnnn_ Cuts at: 1609 1886 2075 3713 4271 5390 1609 Size: 277 189 1638 558 1119 5275 Fragments arranged by size: 5275 1638 1119 558 277 189 BciVI GTATCCnnnnn_n' Cuts at: 2289 6954 8010 9056 2289 Size: 4665 1056 1046 2289 Fragments arranged by size: 4665 2289 1056 1046 BclI T'GATC_A Cuts at: 639 1212 4722 5232 6576 639 Size: 573 3510 510 1344 3119 Fragments arranged by size: 3510 3119 1344 573 510 BfrBI ATG'CAT Cuts at: 1470 2112 4570 4695 5468 1470 Size: 642 2458 125 773 5058 Fragments arranged by size: 5058 2458 773 642 125 BglII A'GATC_T Cuts at: 594 607 1258 3124 4422 5160 6571 8608 594 Size: 13 651 1866 1298 738 1411 2037 1042 Fragments arranged by size: 2037 1866 1411 1298 1042 738 651 13 BlpI GC'TnA_GC Cuts at: 6168 6168 Size: 9056 Bme1580I G_kGCm'C Cuts at: 6681 6774 7898 6681 Size: 93 1124 7839 Fragments arranged by size: 7839 1124 93 BmgBI CAC'GTC Cuts at: 3022 4747 3022 Size: 1725 7331 BmrI ACTGGGnnnn_n' Cuts at: 5504 6268 6349 6684 8828 5504 Size: 764 81 335 2144 5732 Fragments arranged by size: 5732 2144 764 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 837 7423 837 Size: 6586 2470 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1109 1272 2536 3762 4112 4618 4646 5270 1109 Size: 163 1264 1226 350 506 28 624 4895 Fragments arranged by size: 4895 1264 1226 624 506 350 163 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2723 4576 6232 7111 7825 8123 2723 Size: 1853 1656 879 714 298 3656 Fragments arranged by size: 3656 1853 1656 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 1075 2652 2694 6003 8760 1075 Size: 1577 42 3309 2757 1371 Fragments arranged by size: 3309 2757 1577 1371 42 BsaAI yAC'GTr Cuts at: 5157 6409 5157 Size: 1252 7804 BsaBI GATnn'nnATC Cuts at: 6 674 1407 2047 4469 5630 5984 6570 8607 Size: 668 733 640 2422 1161 354 586 2037 Cuts at: 8607 6 Size: 455 Fragments arranged by size: 2422 2037 1161 733 668 640 586 455 354 BsaHI Gr'CG_yC Cuts at: 6487 6735 7437 8933 6487 Size: 248 702 1496 6610 Fragments arranged by size: 6610 1496 702 248 BsaWI w'CCGG_w Cuts at: 6245 6766 7444 7855 8002 8276 6245 Size: 521 678 411 147 274 7025 Fragments arranged by size: 7025 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1791 1821 5490 5520 1791 Size: 30 3669 30 5327 Fragments arranged by size: 5327 3669 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 158 158 Size: 9056 BseYI C'CCAG_C Cuts at: 208 3941 6481 7239 7904 8312 208 Size: 3733 2540 758 665 408 952 Fragments arranged by size: 3733 2540 952 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 756 756 Size: 9056 BsiEI CG_ry'CG Cuts at: 104 6598 6644 7874 104 Size: 6494 46 1230 1286 Fragments arranged by size: 6494 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 3163 4533 4957 5274 6070 6848 7038 7898 3163 Size: 1370 424 317 796 778 190 860 4321 Fragments arranged by size: 4321 1370 860 796 778 424 317 190 BsmI GAATG_Cn' Cuts at: 3630 3630 Size: 9056 Bsp1286I G_dGCh'C Cuts at: 2126 3163 4533 4957 5274 6070 6681 6774 6848 Size: 1037 1370 424 317 796 611 93 74 Cuts at: 6848 7038 7100 7898 2126 Size: 190 62 798 3284 Fragments arranged by size: 3284 1370 1037 798 796 611 424 317 190 93 74 62 BspHI T'CATG_A Cuts at: 2613 4941 7488 2613 Size: 2328 2547 4181 Fragments arranged by size: 4181 2547 2328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 6106 6320 6622 7003 6106 Size: 214 302 381 8159 Fragments arranged by size: 8159 381 302 214 BsrBI CCG'CTC Cuts at: 50 7348 7402 8886 9051 50 Size: 7298 54 1484 165 55 Fragments arranged by size: 7298 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1730 4291 6968 1730 Size: 2561 2677 3818 Fragments arranged by size: 3818 2677 2561 BsrFI r'CCGG_y Cuts at: 4296 7054 7235 4296 Size: 2758 181 6117 Fragments arranged by size: 6117 2758 181 BsrGI T'GTAC_A Cuts at: 672 2863 4177 672 Size: 2191 1314 5551 Fragments arranged by size: 5551 2191 1314 BssSI C'ACGA_G Cuts at: 7327 8035 7327 Size: 708 8348 BstAPI GCAn_nnn'nTGC Cuts at: 4397 5824 6433 4397 Size: 1427 609 7020 Fragments arranged by size: 7020 1427 609 BstBI TT'CG_AA Cuts at: 546 1015 7417 546 Size: 469 6402 2185 Fragments arranged by size: 6402 2185 469 BstXI CCAn_nnnn'nTGG Cuts at: 5196 5196 Size: 9056 BstYI r'GATC_y Cuts at: 1 287 594 607 1258 3124 4422 5160 5172 Size: 286 307 13 651 1866 1298 738 12 Cuts at: 5172 5772 6081 6548 6571 6906 7152 7458 7470 Size: 600 309 467 23 335 246 306 12 Cuts at: 7470 7556 8608 1 Size: 86 1052 449 Fragments arranged by size: 1866 1298 1052 738 651 600 467 449 335 309 307 306 286 246 86 23 13 12 12 BtgI C'CryG_G Cuts at: 107 1976 107 Size: 1869 7187 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 4433 7006 7187 7350 4433 Size: 2573 181 163 6139 Fragments arranged by size: 6139 2573 181 163 BtsI GCAGTG_nn' Cuts at: 688 768 2033 4092 6299 688 Size: 80 1265 2059 2207 3445 Fragments arranged by size: 3445 2207 2059 1265 80 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1854 1889 1854 Size: 35 9021 DraI TTT'AAA Cuts at: 390 1023 1493 1640 4050 4484 4884 5261 5462 Size: 633 470 147 2410 434 400 377 201 Cuts at: 5462 5949 6055 390 Size: 487 106 3391 Fragments arranged by size: 3391 2410 633 487 470 434 400 377 201 147 106 DraIII CAC_nnn'GTG Cuts at: 3022 3022 Size: 9056 DrdI GACnn_nn'nnGTC Cuts at: 6762 8106 6762 Size: 1344 7712 EaeI y'GGCC_r Cuts at: 6641 6815 7206 7233 8578 6641 Size: 174 391 27 1345 7119 Fragments arranged by size: 7119 1345 391 174 27 EagI C'GGCC_G Cuts at: 6641 6641 Size: 9056 EarI CTCTTCn'nnn_ Cuts at: 278 834 1071 1328 1335 2024 2114 3210 3576 Size: 556 237 257 7 689 90 1096 366 Cuts at: 3576 3851 4181 4556 4770 7079 7289 278 Size: 275 330 375 214 2309 210 2045 Fragments arranged by size: 2309 2045 1096 689 556 375 366 330 275 257 237 214 210 90 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 7264 8006 8152 8866 7264 Size: 742 146 714 7454 Fragments arranged by size: 7454 742 714 146 EcoICRI GAG'CTC Cuts at: 4955 6068 4955 Size: 1113 7943 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 317 1109 1272 1535 1718 2357 2536 2539 3762 Size: 792 163 263 183 639 179 3 1223 Cuts at: 3762 4112 4265 4477 4535 4618 4646 4991 5270 Size: 350 153 212 58 83 28 345 279 Cuts at: 5270 5741 6881 7313 7666 8328 317 Size: 471 1140 432 353 662 1045 Fragments arranged by size: 1223 1140 1045 792 662 639 471 432 353 350 345 279 263 212 183 179 163 153 83 58 28 3 EcoO109I rG'GnC_Cy Cuts at: 1764 5754 6195 1764 Size: 3990 441 4625 Fragments arranged by size: 4625 3990 441 EcoRI G'AATT_C Cuts at: 2886 5203 6060 2886 Size: 2317 857 5882 Fragments arranged by size: 5882 2317 857 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 755 3570 3619 4649 7075 755 Size: 2815 49 1030 2426 2736 Fragments arranged by size: 2815 2736 2426 1030 49 FspI TGC'GCA Cuts at: 6837 6837 Size: 9056 HaeII r_GCGC'y Cuts at: 239 3864 6490 6738 7968 8739 239 Size: 3625 2626 248 1230 771 556 Fragments arranged by size: 3625 2626 1230 771 556 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 175 207 586 618 1876 1908 2488 2520 2611 Size: 32 379 32 1258 32 580 32 91 Cuts at: 2611 2643 4228 4260 4483 4515 4555 4587 5527 Size: 32 1585 32 223 32 40 32 940 Cuts at: 5527 5539 5559 5571 5757 5764 5789 5796 5864 Size: 12 20 12 186 7 25 7 68 Cuts at: 5864 5896 6898 6928 6930 6960 175 Size: 32 1002 30 2 30 2271 Fragments arranged by size: 2271 1585 1258 1002 940 580 379 223 186 91 68 40 32 32 32 32 32 32 32 32 32 30 30 25 20 12 12 7 7 2 HincII GTy'rAC Cuts at: 358 1994 2100 4092 4937 6095 358 Size: 1636 106 1992 845 1158 3319 Fragments arranged by size: 3319 1992 1636 1158 845 106 HindIII A'AGCT_T Cuts at: 3541 3573 3541 Size: 32 9024 HpaI GTT'AAC Cuts at: 4092 4092 Size: 9056 KasI G'GCGC_C Cuts at: 6486 6734 6486 Size: 248 8808 KpnI G_GTAC'C Cuts at: 6076 6076 Size: 9056 MfeI C'AATT_G Cuts at: 2408 2408 Size: 9056 MluI A'CGCG_T Cuts at: 8489 8489 Size: 9056 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 194 3692 4295 4934 5957 7815 7999 194 Size: 3498 603 639 1023 1858 184 1251 Fragments arranged by size: 3498 1858 1251 1023 639 603 184 MscI TGG'CCA Cuts at: 6817 6817 Size: 9056 MslI CAynn'nnrTG Cuts at: 620 2618 2768 3226 4307 4538 5027 7045 7172 Size: 1998 150 458 1081 231 489 2018 127 Cuts at: 7172 620 Size: 2504 Fragments arranged by size: 2504 2018 1998 1081 489 458 231 150 127 MspA1I CmG'CkG Cuts at: 1960 2989 4140 6167 6481 6841 7625 7870 1960 Size: 1029 1151 2027 314 360 784 245 3146 Fragments arranged by size: 3146 2027 1151 1029 784 360 314 245 NaeI GCC'GGC Cuts at: 7237 7237 Size: 9056 NarI GG'CG_CC Cuts at: 6487 6735 6487 Size: 248 8808 NcoI C'CATG_G Cuts at: 107 1976 107 Size: 1869 7187 NdeI CA'TA_TG Cuts at: 1920 1920 Size: 9056 NgoMIV G'CCGG_C Cuts at: 7235 7235 Size: 9056 NsiI A_TGCA'T Cuts at: 1472 2114 4572 4697 5470 1472 Size: 642 2458 125 773 5058 Fragments arranged by size: 5058 2458 773 642 125 NspI r_CATG'y Cuts at: 2882 3070 3231 3253 3949 4699 6109 6609 8212 Size: 188 161 22 696 750 1410 500 1603 Cuts at: 8212 2882 Size: 3726 Fragments arranged by size: 3726 1603 1410 750 696 500 188 161 22 PacI TTA_AT'TAA Cuts at: 3347 3347 Size: 9056 PasI CC'CwG_GG Cuts at: 5797 5797 Size: 9056 PciI A'CATG_T Cuts at: 2878 3066 3227 3249 8208 2878 Size: 188 161 22 4959 3726 Fragments arranged by size: 4959 3726 188 161 22 PflMI CCAn_nnn'nTGG Cuts at: 2583 3022 2583 Size: 439 8617 PfoI T'CCnGG_A Cuts at: 4639 4639 Size: 9056 PmeI GTTT'AAAC Cuts at: 6055 6055 Size: 9056 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7502 7534 7502 Size: 32 9024 PpuMI rG'GwC_Cy Cuts at: 1764 1764 Size: 9056 PsiI TTA'TAA Cuts at: 3722 3722 Size: 9056 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2699 2731 3461 3493 2699 Size: 32 730 32 8262 Fragments arranged by size: 8262 730 32 32 PstI C_TGCA'G Cuts at: 6103 6788 6103 Size: 685 8371 PvuI CG_AT'CG Cuts at: 104 104 Size: 9056 PvuII CAG'CTG Cuts at: 1960 2989 4140 6481 6841 1960 Size: 1029 1151 2341 360 4175 Fragments arranged by size: 4175 2341 1151 1029 360 RsrII CG'GwC_CG Cuts at: 7251 7251 Size: 9056 SacI G_AGCT'C Cuts at: 4957 6070 4957 Size: 1113 7943 SalI G'TCGA_C Cuts at: 6093 6093 Size: 9056 SapI GCTCTTCn'nnn_ Cuts at: 2024 2114 3851 4181 7079 7289 2024 Size: 90 1737 330 2898 210 3791 Fragments arranged by size: 3791 2898 1737 330 210 90 SbfI CC_TGCA'GG Cuts at: 6103 6103 Size: 9056 ScaI AGT'ACT Cuts at: 738 909 3033 8573 738 Size: 171 2124 5540 1221 Fragments arranged by size: 5540 2124 1221 171 SfcI C'TryA_G Cuts at: 33 1586 2705 5306 6099 6784 7752 7943 33 Size: 1553 1119 2601 793 685 968 191 1146 Fragments arranged by size: 2601 1553 1146 1119 968 793 685 191 SfoI GGC'GCC Cuts at: 6488 6736 6488 Size: 248 8808 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 9056 SmaI CCC'GGG Cuts at: 6078 6078 Size: 9056 SmlI C'TyrA_G Cuts at: 172 439 903 2738 3427 3544 4153 4555 6211 Size: 267 464 1835 689 117 609 402 1656 Cuts at: 6211 7126 7840 8102 172 Size: 915 714 262 1126 Fragments arranged by size: 1835 1656 1126 915 714 689 609 464 402 267 262 117 SnaBI TAC'GTA Cuts at: 5157 5157 Size: 9056 SpeI A'CTAG_T Cuts at: 67 67 Size: 9056 SphI G_CATG'C Cuts at: 3949 4699 6109 6609 3949 Size: 750 1410 500 6396 Fragments arranged by size: 6396 1410 750 500 SspI AAT'ATT Cuts at: 450 4046 5279 450 Size: 3596 1233 4227 Fragments arranged by size: 4227 3596 1233 StuI AGG'CCT Cuts at: 5457 5457 Size: 9056 StyI C'CwwG_G Cuts at: 107 931 1976 3866 6190 6544 107 Size: 824 1045 1890 2324 354 2619 Fragments arranged by size: 2619 2324 1890 1045 824 354 SwaI ATTT'AAAT Cuts at: 5261 5261 Size: 9056 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 4027 6639 6772 7231 7440 4027 Size: 2612 133 459 209 5643 Fragments arranged by size: 5643 2612 459 209 133 TatI w'GTAC_w Cuts at: 672 736 907 2863 3031 3873 4096 4177 8571 Size: 64 171 1956 168 842 223 81 4394 Cuts at: 8571 672 Size: 1157 Fragments arranged by size: 4394 1956 1157 842 223 171 168 81 64 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5384 5416 5490 5522 8573 8605 5384 Size: 32 74 32 3051 32 5835 Fragments arranged by size: 5835 3051 74 32 32 32 Tth111I GACn'n_nGTC Cuts at: 6853 6853 Size: 9056 XbaI T'CTAG_A Cuts at: 604 1329 2541 6087 604 Size: 725 1212 3546 3573 Fragments arranged by size: 3573 3546 1212 725 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6551 6551 Size: 9056 XmaI C'CCGG_G Cuts at: 6076 6076 Size: 9056 XmnI GAAnn'nnTTC Cuts at: 5141 5619 5141 Size: 478 8578 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflII AflIII AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PsiI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AgeI AhdI AleI ApaI AscI AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I BsiWI BsmBI BspEI BssHII BstEII BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI NheI NotI NruI PmlI PshAI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SrfI XhoI ZraI