Restriction map for
pF1KA1319
(Circular) (Six-base) MAPSORT of: pF1KA1319.seq Check: 9585 from: 1 to: 6722
pF1KA1319 6722 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 149 3325 3656 3760 149
Size: 3176 331 104 3111
Fragments arranged by size:
3176 3111 331 104
Acc65I G'GTAC_C
Cuts at: 3738 3738
Size: 6722
AclI AA'CG_TT
Cuts at: 6562 6562
Size: 6722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 913 1525 1627 1784 2365 2824 3019 3217
Size: 293 612 102 157 581 459 195 198
Cuts at: 3217 3317 4547 4979 5726 5994 620
Size: 100 1230 432 747 268 1348
Fragments arranged by size:
1348 1230 747 612 581 459 432 293
268 198 195 157 102 100
AflII C'TTAA_G
Cuts at: 2029 2029
Size: 6722
AflIII A'CryG_T
Cuts at: 1467 1656 4074 5178 6155 1467
Size: 189 2418 1104 977 2034
Fragments arranged by size:
2418 2034 1104 977 189
AgeI A'CCGG_T
Cuts at: 5110 5110
Size: 6722
AhdI GACnn_n'nnGTC
Cuts at: 2357 2357
Size: 6722
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 589 1339 1720 589
Size: 750 381 5591
Fragments arranged by size:
5591 750 381
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2141 2173 3350 3382 2141
Size: 32 1177 32 5481
Fragments arranged by size:
5481 1177 32 32
AlwNI CAG_nnn'CTG
Cuts at: 538 876 1332 2184 2343 2415 2436 2562 2859
Size: 338 456 852 159 72 21 126 297
Cuts at: 2859 2871 2934 2943 3153 3601 3713 4027 5594
Size: 12 63 9 210 448 112 314 1567
Cuts at: 5594 538
Size: 1666
Fragments arranged by size:
1666 1567 852 456 448 338 314 297
210 159 126 112 72 63 21 12
9
ApaI G_GGCC'C
Cuts at: 145 1617 2574 2991 145
Size: 1472 957 417 3876
Fragments arranged by size:
3876 1472 957 417
ApaLI G'TGCA_C
Cuts at: 956 5492 956
Size: 4536 2186
ApoI r'AATT_y
Cuts at: 2308 3643 3726 6075 6331 2308
Size: 1335 83 2349 256 2699
Fragments arranged by size:
2699 2349 1335 256 83
AseI AT'TA_AT
Cuts at: 20 20
Size: 6722
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6722
AvaI C'yCGr_G
Cuts at: 137 686 1523 1958 2354 3007 3742 137
Size: 549 837 435 396 653 735 3117
Fragments arranged by size:
3117 837 735 653 549 435 396
BamHI G'GATC_C
Cuts at: 549 3747 549
Size: 3198 3524
BanI G'GyrC_C
Cuts at: 117 3738 4152 4400 4435 117
Size: 3621 414 248 35 2404
Fragments arranged by size:
3621 2404 414 248 35
BanII G_rGCy'C
Cuts at: 137 145 387 394 1379 1617 2574 2991 3125
Size: 8 242 7 985 238 957 417 134
Cuts at: 3125 3634 3736 4766 137
Size: 509 102 1030 2093
Fragments arranged by size:
2093 1030 985 957 509 417 242 238
134 102 8 7
BbeI G_GCGC'C
Cuts at: 4156 4404 4156
Size: 248 6474
BbsI GAAGACnn'nnnn_
Cuts at: 626 2821 3205 3316 3383 626
Size: 2195 384 111 67 3965
Fragments arranged by size:
3965 2195 384 111 67
BbvCI CC'TCA_GC
Cuts at: 1576 3112 1576
Size: 1536 5186
BciVI GTATCCnnnnn_n'
Cuts at: 4620 5381 6722 4620
Size: 761 1341 4620
Fragments arranged by size:
4620 1341 761
BclI T'GATC_A
Cuts at: 4242 4242
Size: 6722
BglI GCCn_nnn'nGGC
Cuts at: 529 2494 2806 2875 529
Size: 1965 312 69 4376
Fragments arranged by size:
4376 1965 312 69
BglII A'GATC_T
Cuts at: 4237 6274 4237
Size: 2037 4685
BlpI GC'TnA_GC
Cuts at: 131 2522 2621 2888 3415 3594 3628 3834 131
Size: 2391 99 267 527 179 34 206 3019
Fragments arranged by size:
3019 2391 527 267 206 179 99 34
Bme1580I G_kGCm'C
Cuts at: 145 208 847 960 1617 2499 2574 2991 4347
Size: 63 639 113 657 882 75 417 1356
Cuts at: 4347 4440 5496 145
Size: 93 1056 1371
Fragments arranged by size:
1371 1356 1056 882 657 639 417 113
93 75 63
BmrI ACTGGGnnnn_n'
Cuts at: 538 930 3934 4015 4350 6494 538
Size: 392 3004 81 335 2144 766
Fragments arranged by size:
3004 2144 766 392 335 81
BmtI G_CTAG'C
Cuts at: 586 586
Size: 6722
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1101 1650 2835 3588 5089 1101
Size: 549 1185 753 1501 2734
Fragments arranged by size:
2734 1501 1185 753 549
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 977 1150 1303 1315 1357 1363 1450 1756
Size: 379 173 153 12 42 6 87 306
Cuts at: 1756 1810 1991 2143 2161 2437 2458 2521 2596
Size: 54 181 152 18 276 21 63 75
Cuts at: 2596 2647 2707 2755 2843 2965 3175 3361 3526
Size: 51 60 48 88 122 210 186 165
Cuts at: 3526 3583 598
Size: 57 3737
Fragments arranged by size:
3737 379 306 276 210 186 181 173
165 153 152 122 88 87 75 63
60 57 54 51 48 42 21 18
12 6
Bpu10I CC'TnA_GC
Cuts at: 236 1481 1490 1576 3112 236
Size: 1245 9 86 1536 3846
Fragments arranged by size:
3846 1536 1245 86 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1981 3277 3898 4777 5269 5567 1981
Size: 1296 621 879 492 298 3136
Fragments arranged by size:
3136 1296 879 621 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1001 1426 1513 2281 3010 6426 1001
Size: 425 87 768 729 3416 1297
Fragments arranged by size:
3416 1297 768 729 425 87
BsaAI yAC'GTr
Cuts at: 4075 4075
Size: 6722
BsaBI GATnn'nnATC
Cuts at: 6 4236 6273 6
Size: 4230 2037 455
Fragments arranged by size:
4230 2037 455
BsaHI Gr'CG_yC
Cuts at: 228 4153 4401 5103 6599 228
Size: 3925 248 702 1496 351
Fragments arranged by size:
3925 1496 702 351 248
BsaWI w'CCGG_w
Cuts at: 2012 3911 4432 5110 5384 5531 5942 2012
Size: 1899 521 678 274 147 411 2792
Fragments arranged by size:
2792 1899 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 781 811 1579 1609 2141 2171 3154 3184 781
Size: 30 768 30 532 30 983 30 4319
Fragments arranged by size:
4319 983 768 532 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 487 955 966 1122 1228 1312 1356 1582 1639
Size: 468 11 156 106 84 44 226 57
Cuts at: 1639 1807 1819 1912 1924 2038 2455 2482 2530
Size: 168 12 93 12 114 417 27 48
Cuts at: 2530 2593 2644 3061 3529 487
Size: 63 51 417 468 3680
Fragments arranged by size:
3680 468 468 417 417 226 168 156
114 106 93 84 63 57 51 48
44 27 12 12 11
BseYI C'CCAG_C
Cuts at: 231 289 1358 2068 2247 2729 2777 2927 3500
Size: 58 1069 710 179 482 48 150 573
Cuts at: 3500 4147 4905 5482 5978 231
Size: 647 758 577 496 975
Fragments arranged by size:
1069 975 758 710 647 577 573 496
482 179 150 58 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 298 1335 215
Size: 83 1037 5602
Fragments arranged by size:
5602 1037 83
BsiEI CG_ry'CG
Cuts at: 104 4264 4310 5518 104
Size: 4160 46 1208 1308
Fragments arranged by size:
4160 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 311 387 394 960 1379 1997 2606 3125 3634
Size: 76 7 566 419 618 609 519 509
Cuts at: 3634 3736 4514 4704 5496 311
Size: 102 778 190 792 1537
Fragments arranged by size:
1537 792 778 618 609 566 519 509
419 190 102 76 7
BsmI GAATG_Cn'
Cuts at: 2457 2457
Size: 6722
BsmBI CGTCTCn'nnnn_
Cuts at: 2266 2266
Size: 6722
Bsp1286I G_dGCh'C
Cuts at: 137 145 208 311 387 394 847 960 1379
Size: 8 63 103 76 7 453 113 419
Cuts at: 1379 1617 1997 2499 2574 2606 2991 3125 3634
Size: 238 380 502 75 32 385 134 509
Cuts at: 3634 3736 4347 4440 4514 4704 4766 5496 137
Size: 102 611 93 74 190 62 730 1363
Fragments arranged by size:
1363 730 611 509 502 453 419 385
380 238 190 134 113 103 102 93
76 75 74 63 62 32 8 7
BspEI T'CCGG_A
Cuts at: 2012 5942 2012
Size: 3930 2792
BspHI T'CATG_A
Cuts at: 5898 5898
Size: 6722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3772 3986 4288 4669 3772
Size: 214 302 381 5825
Fragments arranged by size:
5825 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1287 1590 2892 3285 3345 3384 3593 5014
Size: 1237 303 1302 393 60 39 209 1421
Cuts at: 5014 5068 6552 6717 50
Size: 54 1484 165 55
Fragments arranged by size:
1484 1421 1302 1237 393 303 209 165
60 55 54 39
BsrDI GCAATG_nn'
Cuts at: 3671 4634 3671
Size: 963 5759
BsrFI r'CCGG_y
Cuts at: 2315 4720 4901 5110 2315
Size: 2405 181 209 3927
Fragments arranged by size:
3927 2405 209 181
BssSI C'ACGA_G
Cuts at: 4993 5351 4993
Size: 358 6364
BstAPI GCAn_nnn'nTGC
Cuts at: 1332 2712 2871 4099 1332
Size: 1380 159 1228 3955
Fragments arranged by size:
3955 1380 1228 159
BstBI TT'CG_AA
Cuts at: 850 5083 850
Size: 4233 2489
BstYI r'GATC_y
Cuts at: 1 549 1848 2849 3747 4214 4237 4572 4818
Size: 548 1299 1001 898 467 23 335 246
Cuts at: 4818 5830 5916 5928 6274 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1299 1012 1001 898 548 467 449 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 378 1072 2113 3421 3487 378
Size: 694 1041 1308 66 3613
Fragments arranged by size:
3613 1308 1041 694 66
BtgI C'CryG_G
Cuts at: 107 153 766 946 1178 3059 107
Size: 46 613 180 232 1881 3770
Fragments arranged by size:
3770 1881 613 232 180 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4672 4853 5016 4672
Size: 181 163 6378
Fragments arranged by size:
6378 181 163
BtsI GCAGTG_nn'
Cuts at: 620 1041 1736 2273 3679 3965 620
Size: 421 695 537 1406 286 3377
Fragments arranged by size:
3377 1406 695 537 421 286
DraI TTT'AAA
Cuts at: 3721 3721
Size: 6722
DrdI GACnn_nn'nnGTC
Cuts at: 157 4428 5286 157
Size: 4271 858 1593
Fragments arranged by size:
4271 1593 858
EaeI y'GGCC_r
Cuts at: 699 2225 3090 3209 4307 4481 4872 4899 6244
Size: 1526 865 119 1098 174 391 27 1345
Cuts at: 6244 699
Size: 1177
Fragments arranged by size:
1526 1345 1177 1098 865 391 174 119
27
EagI C'GGCC_G
Cuts at: 4307 4307
Size: 6722
EarI CTCTTCn'nnn_
Cuts at: 1232 1304 1532 1901 2195 2459 3293 3455 4745
Size: 72 228 369 294 264 834 162 1290
Cuts at: 4745 4955 1232
Size: 210 2999
Fragments arranged by size:
2999 1290 834 369 294 264 228 210
162 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2302 4930 5240 5386 6532 2302
Size: 2628 310 146 1146 2492
Fragments arranged by size:
2628 2492 1146 310 146
EcoICRI GAG'CTC
Cuts at: 385 392 1377 3123 3632 3734 385
Size: 7 985 1746 509 102 3373
Fragments arranged by size:
3373 1746 985 509 102 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 620 913 977 1150 1303 1315 1357 1363
Size: 22 293 64 173 153 12 42 6
Cuts at: 1363 1450 1525 1627 1756 1784 1810 1991 2143
Size: 87 75 102 129 28 26 181 152
Cuts at: 2143 2161 2365 2437 2458 2521 2596 2647 2707
Size: 18 204 72 21 63 75 51 60
Cuts at: 2707 2755 2824 2843 2965 3019 3175 3217 3317
Size: 48 69 19 122 54 156 42 100
Cuts at: 3317 3361 3526 3583 4547 4979 5726 5994 598
Size: 44 165 57 964 432 747 268 1326
Fragments arranged by size:
1326 964 747 432 293 268 204 181
173 165 156 153 152 129 122 102
100 87 75 75 72 69 64 63
60 57 54 51 48 44 42 42
28 26 22 21 19 18 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1075 1104 1621 2515 2545 2578 1075
Size: 29 517 894 30 33 5219
Fragments arranged by size:
5219 894 517 33 30 29
EcoO109I rG'GnC_Cy
Cuts at: 141 142 374 482 535 924 963 1477 1509
Size: 1 232 108 53 389 39 514 32
Cuts at: 1509 1613 1614 2445 2571 2868 2987 3150 3192
Size: 104 1 831 126 297 119 163 42
Cuts at: 3192 3861 141
Size: 669 3002
Fragments arranged by size:
3002 831 669 514 389 297 232 163
126 119 108 104 53 42 39 32
1 1
EcoRI G'AATT_C
Cuts at: 2308 3643 3726 2308
Size: 1335 83 5304
Fragments arranged by size:
5304 1335 83
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1413 1930 4741 1413
Size: 517 2811 3394
Fragments arranged by size:
3394 2811 517
FspI TGC'GCA
Cuts at: 4503 4503
Size: 6722
HaeII r_GCGC'y
Cuts at: 795 1395 2784 4156 4404 5426 6405 795
Size: 600 1389 1372 248 1022 979 1112
Fragments arranged by size:
1389 1372 1112 1022 979 600 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 192 224 781 813 1128 1160 2343 2375 3154
Size: 32 557 32 315 32 1183 32 779
Cuts at: 3154 3186 3543 3575 3652 3684 4564 4594 4596
Size: 32 357 32 77 32 880 30 2
Cuts at: 4596 4626 192
Size: 30 2288
Fragments arranged by size:
2288 1183 880 779 557 357 315 77
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 670 1598 3761 670
Size: 928 2163 3631
Fragments arranged by size:
3631 2163 928
HindIII A'AGCT_T
Cuts at: 3584 3584
Size: 6722
KasI G'GCGC_C
Cuts at: 4152 4400 4152
Size: 248 6474
KpnI G_GTAC'C
Cuts at: 3742 3742
Size: 6722
MluI A'CGCG_T
Cuts at: 6155 6155
Size: 6722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 673 2072 2660 5393 5577 673
Size: 1399 588 2733 184 1818
Fragments arranged by size:
2733 1818 1399 588 184
MscI TGG'CCA
Cuts at: 3211 4483 3211
Size: 1272 5450
MslI CAynn'nnrTG
Cuts at: 3395 4711 4838 3395
Size: 1316 127 5279
Fragments arranged by size:
5279 1316 127
MspA1I CmG'CkG
Cuts at: 948 1293 1575 1881 2103 2256 2343 2412 2436
Size: 345 282 306 222 153 87 69 24
Cuts at: 2436 2526 2595 2733 2997 3111 3598 3833 4147
Size: 90 69 138 264 114 487 235 314
Cuts at: 4147 4507 5520 5765 948
Size: 360 1013 245 1905
Fragments arranged by size:
1905 1013 487 360 345 314 306 282
264 245 235 222 153 138 114 90
87 69 69 24
NaeI GCC'GGC
Cuts at: 2317 4903 2317
Size: 2586 4136
NarI GG'CG_CC
Cuts at: 4153 4401 4153
Size: 248 6474
NcoI C'CATG_G
Cuts at: 107 153 107
Size: 46 6676
NdeI CA'TA_TG
Cuts at: 1046 1046
Size: 6722
NgoMIV G'CCGG_C
Cuts at: 2315 4901 2315
Size: 2586 4136
NheI G'CTAG_C
Cuts at: 582 582
Size: 6722
NspI r_CATG'y
Cuts at: 576 1471 1660 1707 3394 3775 4275 5182 576
Size: 895 189 47 1687 381 500 907 2116
Fragments arranged by size:
2116 1687 907 895 500 381 189 47
PciI A'CATG_T
Cuts at: 1467 1656 5178 1467
Size: 189 3522 3011
Fragments arranged by size:
3522 3011 189
PflMI CCAn_nnn'nTGG
Cuts at: 190 809 1443 1650 2064 2795 190
Size: 619 634 207 414 731 4117
Fragments arranged by size:
4117 731 634 619 414 207
PmeI GTTT'AAAC
Cuts at: 3721 3721
Size: 6722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 406 438 2141 2173 5861 5893 406
Size: 32 1703 32 3688 32 1235
Fragments arranged by size:
3688 1703 1235 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 924 963 1477 1509 3150 3192 924
Size: 39 514 32 1641 42 4454
Fragments arranged by size:
4454 1641 514 42 39 32
PsiI TTA'TAA
Cuts at: 2744 2744
Size: 6722
PspOMI G'GGCC_C
Cuts at: 141 1613 2570 2987 141
Size: 1472 957 417 3876
Fragments arranged by size:
3876 1472 957 417
PspXI vC'TCGA_Gb
Cuts at: 2354 2354
Size: 6722
PstI C_TGCA'G
Cuts at: 980 1328 1454 1781 1880 2042 2411 2717 2876
Size: 348 126 327 99 162 369 306 159
Cuts at: 2876 2939 3323 3769 4454 980
Size: 63 384 446 685 3248
Fragments arranged by size:
3248 685 446 384 369 348 327 306
162 159 126 99 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6722
PvuII CAG'CTG
Cuts at: 1293 1575 1881 2103 2343 2436 2733 2997 3111
Size: 282 306 222 240 93 297 264 114
Cuts at: 3111 3598 4147 4507 1293
Size: 487 549 360 3508
Fragments arranged by size:
3508 549 487 360 306 297 282 264
240 222 114 93
RsrII CG'GwC_CG
Cuts at: 4917 4917
Size: 6722
SacI G_AGCT'C
Cuts at: 387 394 1379 3125 3634 3736 387
Size: 7 985 1746 509 102 3373
Fragments arranged by size:
3373 1746 985 509 102 7
SacII CC_GC'GG
Cuts at: 949 949
Size: 6722
SalI G'TCGA_C
Cuts at: 3759 3759
Size: 6722
SapI GCTCTTCn'nnn_
Cuts at: 4745 4955 4745
Size: 210 6512
SbfI CC_TGCA'GG
Cuts at: 2876 3769 2876
Size: 893 5829
ScaI AGT'ACT
Cuts at: 6239 6239
Size: 6722
SexAI A'CCwGG_T
Cuts at: 3098 3098
Size: 6722
SfcI C'TryA_G
Cuts at: 33 976 1219 1324 1450 1777 1876 2038 2407
Size: 943 243 105 126 327 99 162 369
Cuts at: 2407 2713 2872 2935 3289 3319 3700 3765 4450
Size: 306 159 63 354 30 381 65 685
Cuts at: 4450 5443 5634 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 943 685 381 369 354 327
306 243 191 162 159 126 105 99
65 63 30
SfoI GGC'GCC
Cuts at: 4154 4402 4154
Size: 248 6474
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6722
SgrAI Cr'CCGG_yG
Cuts at: 2315 2315
Size: 6722
SmaI CCC'GGG
Cuts at: 139 688 1525 3744 139
Size: 549 837 2219 3117
Fragments arranged by size:
3117 2219 837 549
SmlI C'TyrA_G
Cuts at: 1996 2029 2354 3007 3256 3877 4792 5284 5546
Size: 33 325 653 249 621 915 492 262
Cuts at: 5546 1996
Size: 3172
Fragments arranged by size:
3172 915 653 621 492 325 262 249
33
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6722
SphI G_CATG'C
Cuts at: 576 1707 3775 4275 576
Size: 1131 2068 500 3023
Fragments arranged by size:
3023 2068 1131 500
StuI AGG'CCT
Cuts at: 523 523
Size: 6722
StyI C'CwwG_G
Cuts at: 107 153 369 2210 2789 3173 3856 4210 107
Size: 46 216 1841 579 384 683 354 2619
Fragments arranged by size:
2619 1841 683 579 384 354 216 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 135 2678 4305 4438 4897 5106 135
Size: 2543 1627 133 459 209 1751
Fragments arranged by size:
2543 1751 1627 459 209 133
TatI w'GTAC_w
Cuts at: 6237 6237
Size: 6722
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3142 3174 6239 6271 3142
Size: 32 3065 32 3593
Fragments arranged by size:
3593 3065 32 32
Tth111I GACn'n_nGTC
Cuts at: 922 3652 4519 922
Size: 2730 867 3125
Fragments arranged by size:
3125 2730 867
XbaI T'CTAG_A
Cuts at: 3753 3753
Size: 6722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2065 2217 2496 3336 4217 2065
Size: 152 279 840 881 4570
Fragments arranged by size:
4570 881 840 279 152
XhoI C'TCGA_G
Cuts at: 2354 3007 2354
Size: 653 6069
XmaI C'CCGG_G
Cuts at: 137 686 1523 3742 137
Size: 549 837 2219 3117
Fragments arranged by size:
3117 2219 837 549
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NspI PciI PflMI
PmeI PpiI PpuMI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AleI AscI AvrII BaeI BcgI
BfrBI BmgBI BsiWI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsrI SanDI SfiI SnaBI SrfI SspI SwaI XmnI
ZraI