Restriction map for
pF1KA1321
(Circular) (Six-base) MAPSORT of: pF1KA1321.seq Check: 6947 from: 1 to: 5198
pF1KA1321 5198 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 500 2236 500
Size: 1736 3462
Acc65I G'GTAC_C
Cuts at: 2214 2214
Size: 5198
AclI AA'CG_TT
Cuts at: 5038 5038
Size: 5198
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 1064 1472 3023 3455 4202 4470 532
Size: 532 408 1551 432 747 268 1260
Fragments arranged by size:
1551 1260 747 532 432 408 268
AfeI AGC'GCT
Cuts at: 1322 1322
Size: 5198
AflIII A'CryG_T
Cuts at: 2550 3654 4631 2550
Size: 1104 977 3117
Fragments arranged by size:
3117 1104 977
AgeI A'CCGG_T
Cuts at: 3586 3586
Size: 5198
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1764 1796 1764
Size: 32 5166
AlwNI CAG_nnn'CTG
Cuts at: 1375 1410 1495 1774 2503 4070 1375
Size: 35 85 279 729 1567 2503
Fragments arranged by size:
2503 1567 729 279 85 35
ApaLI G'TGCA_C
Cuts at: 3968 3968
Size: 5198
ApoI r'AATT_y
Cuts at: 564 2127 2202 4551 4807 564
Size: 1563 75 2349 256 955
Fragments arranged by size:
2349 1563 955 256 75
AseI AT'TA_AT
Cuts at: 20 20
Size: 5198
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5198
AvaI C'yCGr_G
Cuts at: 2218 2218
Size: 5198
BamHI G'GATC_C
Cuts at: 2223 2223
Size: 5198
BanI G'GyrC_C
Cuts at: 2214 2628 2876 2911 2214
Size: 414 248 35 4501
Fragments arranged by size:
4501 414 248 35
BanII G_rGCy'C
Cuts at: 1652 2212 3242 1652
Size: 560 1030 3608
Fragments arranged by size:
3608 1030 560
BbeI G_GCGC'C
Cuts at: 2632 2880 2632
Size: 248 4950
BbvCI CC'TCA_GC
Cuts at: 137 176 137
Size: 39 5159
BciVI GTATCCnnnnn_n'
Cuts at: 3096 3857 5198 3096
Size: 761 1341 3096
Fragments arranged by size:
3096 1341 761
BclI T'GATC_A
Cuts at: 2718 2718
Size: 5198
BglII A'GATC_T
Cuts at: 2713 4750 2713
Size: 2037 3161
BlpI GC'TnA_GC
Cuts at: 2310 2310
Size: 5198
Bme1580I G_kGCm'C
Cuts at: 2823 2916 3972 2823
Size: 93 1056 4049
Fragments arranged by size:
4049 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 968 1123 1646 1685 1886 2410 2491 2826 4970
Size: 155 523 39 201 524 81 335 2144
Cuts at: 4970 968
Size: 1196
Fragments arranged by size:
2144 1196 524 523 335 201 155 81
39
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3565 3565
Size: 5198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 431 644 986 1379 1453 1828 334
Size: 97 213 342 393 74 375 3704
Fragments arranged by size:
3704 393 375 342 213 97 74
Bpu10I CC'TnA_GC
Cuts at: 137 176 137
Size: 39 5159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1811 2374 3253 3745 4043 1811
Size: 563 879 492 298 2966
Fragments arranged by size:
2966 879 563 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 844 4902 844
Size: 4058 1140
BsaAI yAC'GTr
Cuts at: 2551 2551
Size: 5198
BsaBI GATnn'nnATC
Cuts at: 6 2712 4749 6
Size: 2706 2037 455
Fragments arranged by size:
2706 2037 455
BsaHI Gr'CG_yC
Cuts at: 2629 2877 3579 5075 2629
Size: 248 702 1496 2752
Fragments arranged by size:
2752 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2387 2908 3586 3860 4007 4418 2387
Size: 521 678 274 147 411 3167
Fragments arranged by size:
3167 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 332 362 332
Size: 30 5168
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 127
Size: 5198
BseYI C'CCAG_C
Cuts at: 311 2063 2623 3381 3958 4454 311
Size: 1752 560 758 577 496 1055
Fragments arranged by size:
1752 1055 758 577 560 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 847
Size: 5198
BsiEI CG_ry'CG
Cuts at: 104 2740 2786 3994 104
Size: 2636 46 1208 1308
Fragments arranged by size:
2636 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1561 2212 2990 3180 3972 1561
Size: 651 778 190 792 2787
Fragments arranged by size:
2787 792 778 651 190
BsmI GAATG_Cn'
Cuts at: 1841 1841
Size: 5198
Bsp1286I G_dGCh'C
Cuts at: 1561 1652 2212 2823 2916 2990 3180 3242 3972
Size: 91 560 611 93 74 190 62 730
Cuts at: 3972 1561
Size: 2787
Fragments arranged by size:
2787 730 611 560 190 93 91 74
62
BspEI T'CCGG_A
Cuts at: 4418 4418
Size: 5198
BspHI T'CATG_A
Cuts at: 4374 4374
Size: 5198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 287 996 1916 2248 2462 2764 3145 287
Size: 709 920 332 214 302 381 2340
Fragments arranged by size:
2340 920 709 381 332 302 214
BsrBI CCG'CTC
Cuts at: 50 755 3490 3544 5028 5193 50
Size: 705 2735 54 1484 165 55
Fragments arranged by size:
2735 1484 705 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3110 3110
Size: 5198
BsrFI r'CCGG_y
Cuts at: 3196 3377 3586 3196
Size: 181 209 4808
Fragments arranged by size:
4808 209 181
BssSI C'ACGA_G
Cuts at: 1104 3469 3827 1104
Size: 2365 358 2475
Fragments arranged by size:
2475 2365 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1548 2575 1548
Size: 1027 4171
BstBI TT'CG_AA
Cuts at: 934 3559 934
Size: 2625 2573
BstEII G'GTnAC_C
Cuts at: 1903 1903
Size: 5198
BstYI r'GATC_y
Cuts at: 1 745 1572 2142 2223 2690 2713 3048 3294
Size: 744 827 570 81 467 23 335 246
Cuts at: 3294 4306 4392 4404 4750 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 827 744 570 467 449 346 335
246 86 81 23 12
Bsu36I CC'TnA_GG
Cuts at: 1767 2080 1767
Size: 313 4885
BtgI C'CryG_G
Cuts at: 107 2174 107
Size: 2067 3131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3148 3329 3492 3148
Size: 181 163 4854
Fragments arranged by size:
4854 181 163
BtsI GCAGTG_nn'
Cuts at: 1158 1408 2441 1158
Size: 250 1033 3915
Fragments arranged by size:
3915 1033 250
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 656 691 656
Size: 35 5163
DraI TTT'AAA
Cuts at: 431 1119 2197 431
Size: 688 1078 3432
Fragments arranged by size:
3432 1078 688
DraIII CAC_nnn'GTG
Cuts at: 1750 1750
Size: 5198
DrdI GACnn_nn'nnGTC
Cuts at: 2904 3762 2904
Size: 858 4340
EaeI y'GGCC_r
Cuts at: 123 2783 2957 3348 3375 4720 123
Size: 2660 174 391 27 1345 601
Fragments arranged by size:
2660 1345 601 391 174 27
EagI C'GGCC_G
Cuts at: 2783 2783
Size: 5198
EarI CTCTTCn'nnn_
Cuts at: 1230 1452 3221 3431 1230
Size: 222 1769 210 2997
Fragments arranged by size:
2997 1769 222 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3406 3716 3862 5008 3406
Size: 310 146 1146 3596
Fragments arranged by size:
3596 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2210 2210
Size: 5198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 431 532 644 986 1064 1379 1453 1472
Size: 97 101 112 342 78 315 74 19
Cuts at: 1472 1828 3023 3455 4202 4470 334
Size: 356 1195 432 747 268 1062
Fragments arranged by size:
1195 1062 747 432 356 342 315 268
112 101 97 78 74 19
EcoO109I rG'GnC_Cy
Cuts at: 474 1308 2337 474
Size: 834 1029 3335
Fragments arranged by size:
3335 1029 834
EcoRI G'AATT_C
Cuts at: 2202 2202
Size: 5198
EcoRV GAT'ATC
Cuts at: 1611 1611
Size: 5198
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3217 3217
Size: 5198
FspI TGC'GCA
Cuts at: 2979 2979
Size: 5198
HaeII r_GCGC'y
Cuts at: 1324 2632 2880 3902 4881 1324
Size: 1308 248 1022 979 1641
Fragments arranged by size:
1641 1308 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 619 651 1564 1596 1809 1841 1959 1991 3040
Size: 32 913 32 213 32 118 32 1049
Cuts at: 3040 3070 3072 3102 619
Size: 30 2 30 2715
Fragments arranged by size:
2715 1049 913 213 118 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2237 2237
Size: 5198
KasI G'GCGC_C
Cuts at: 2628 2876 2628
Size: 248 4950
KpnI G_GTAC'C
Cuts at: 2218 2218
Size: 5198
MluI A'CGCG_T
Cuts at: 4631 4631
Size: 5198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3869 4053 3869
Size: 184 5014
MscI TGG'CCA
Cuts at: 2959 2959
Size: 5198
MslI CAynn'nnrTG
Cuts at: 1901 3187 3314 1901
Size: 1286 127 3785
Fragments arranged by size:
3785 1286 127
MspA1I CmG'CkG
Cuts at: 324 1084 1495 2309 2623 2983 3996 4241 324
Size: 760 411 814 314 360 1013 245 1281
Fragments arranged by size:
1281 1013 814 760 411 360 314 245
NaeI GCC'GGC
Cuts at: 3379 3379
Size: 5198
NarI GG'CG_CC
Cuts at: 2629 2877 2629
Size: 248 4950
NcoI C'CATG_G
Cuts at: 107 2174 107
Size: 2067 3131
NgoMIV G'CCGG_C
Cuts at: 3377 3377
Size: 5198
NspI r_CATG'y
Cuts at: 1078 2251 2751 3658 1078
Size: 1173 500 907 2618
Fragments arranged by size:
2618 1173 907 500
PciI A'CATG_T
Cuts at: 3654 3654
Size: 5198
PfoI T'CCnGG_A
Cuts at: 1469 1469
Size: 5198
PmeI GTTT'AAAC
Cuts at: 2197 2197
Size: 5198
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4337 4369 4337
Size: 32 5166
PpuMI rG'GwC_Cy
Cuts at: 474 1308 474
Size: 834 4364
PshAI GACnn'nnGTC
Cuts at: 856 1478 856
Size: 622 4576
PsiI TTA'TAA
Cuts at: 1642 1642
Size: 5198
PstI C_TGCA'G
Cuts at: 284 1913 2245 2930 284
Size: 1629 332 685 2552
Fragments arranged by size:
2552 1629 685 332
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5198
PvuII CAG'CTG
Cuts at: 1084 1495 2623 2983 1084
Size: 411 1128 360 3299
Fragments arranged by size:
3299 1128 411 360
RsrII CG'GwC_CG
Cuts at: 3393 3393
Size: 5198
SacI G_AGCT'C
Cuts at: 2212 2212
Size: 5198
SalI G'TCGA_C
Cuts at: 2235 2235
Size: 5198
SapI GCTCTTCn'nnn_
Cuts at: 3221 3431 3221
Size: 210 4988
SbfI CC_TGCA'GG
Cuts at: 2245 2245
Size: 5198
ScaI AGT'ACT
Cuts at: 4715 4715
Size: 5198
SexAI A'CCwGG_T
Cuts at: 1029 1029
Size: 5198
SfcI C'TryA_G
Cuts at: 33 280 638 900 1909 2241 2926 3919 4110
Size: 247 358 262 1009 332 685 993 191
Cuts at: 4110 33
Size: 1121
Fragments arranged by size:
1121 1009 993 685 358 332 262 247
191
SfoI GGC'GCC
Cuts at: 2630 2878 2630
Size: 248 4950
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5198
SmaI CCC'GGG
Cuts at: 2220 2220
Size: 5198
SmlI C'TyrA_G
Cuts at: 1826 2353 3268 3760 4022 1826
Size: 527 915 492 262 3002
Fragments arranged by size:
3002 915 527 492 262
SpeI A'CTAG_T
Cuts at: 67 1819 1861 67
Size: 1752 42 3404
Fragments arranged by size:
3404 1752 42
SphI G_CATG'C
Cuts at: 1078 2251 2751 1078
Size: 1173 500 3525
Fragments arranged by size:
3525 1173 500
StuI AGG'CCT
Cuts at: 2045 2045
Size: 5198
StyI C'CwwG_G
Cuts at: 107 305 800 1793 1883 2174 2332 2686 107
Size: 198 495 993 90 291 158 354 2619
Fragments arranged by size:
2619 993 495 354 291 198 158 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 985 2781 2914 3373 3582 985
Size: 1796 133 459 209 2601
Fragments arranged by size:
2601 1796 459 209 133
TatI w'GTAC_w
Cuts at: 4713 4713
Size: 5198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1848 1880 4715 4747 1848
Size: 32 2835 32 2299
Fragments arranged by size:
2835 2299 32 32
Tth111I GACn'n_nGTC
Cuts at: 2995 2995
Size: 5198
XbaI T'CTAG_A
Cuts at: 2229 2229
Size: 5198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2693 2693
Size: 5198
XmaI C'CCGG_G
Cuts at: 2218 2218
Size: 5198
XmnI GAAnn'nnTTC
Cuts at: 1760 1760
Size: 5198
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmBI BsrGI BssHII BstXI BstZ17I ClaI EcoNI
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI