Restriction map for
pF1KA1331
(Circular) (Six-base) MAPSORT of: pF1KA1331.seq Check: 248 from: 1 to: 4880
pF1KA1331 4880 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1918 1918
Size: 4880
Acc65I G'GTAC_C
Cuts at: 1896 1896
Size: 4880
AclI AA'CG_TT
Cuts at: 4720 4720
Size: 4880
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 374 698 1618 2705 3137 3884 4152 208
Size: 166 324 920 1087 432 747 268 936
Fragments arranged by size:
1087 936 920 747 432 324 268 166
AflIII A'CryG_T
Cuts at: 2232 3336 4313 2232
Size: 1104 977 2799
Fragments arranged by size:
2799 1104 977
AgeI A'CCGG_T
Cuts at: 158 3268 158
Size: 3110 1770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 559 559
Size: 4880
AlwNI CAG_nnn'CTG
Cuts at: 436 1619 1872 2185 3752 436
Size: 1183 253 313 1567 1564
Fragments arranged by size:
1567 1564 1183 313 253
ApaI G_GGCC'C
Cuts at: 183 183
Size: 4880
ApaLI G'TGCA_C
Cuts at: 3650 3650
Size: 4880
ApoI r'AATT_y
Cuts at: 1884 4233 4489 1884
Size: 2349 256 2275
Fragments arranged by size:
2349 2275 256
AseI AT'TA_AT
Cuts at: 20 1355 20
Size: 1335 3545
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
AvaI C'yCGr_G
Cuts at: 176 521 1301 1900 176
Size: 345 780 599 3156
Fragments arranged by size:
3156 780 599 345
AvrII C'CTAG_G
Cuts at: 1245 1245
Size: 4880
BamHI G'GATC_C
Cuts at: 439 1905 439
Size: 1466 3414
BanI G'GyrC_C
Cuts at: 136 165 1518 1896 2310 2558 2593 136
Size: 29 1353 378 414 248 35 2423
Fragments arranged by size:
2423 1353 414 378 248 35 29
BanII G_rGCy'C
Cuts at: 183 1894 2924 183
Size: 1711 1030 2139
Fragments arranged by size:
2139 1711 1030
BbeI G_GCGC'C
Cuts at: 2314 2562 2314
Size: 248 4632
BbsI GAAGACnn'nnnn_
Cuts at: 594 594
Size: 4880
BciVI GTATCCnnnnn_n'
Cuts at: 1042 2778 3539 4880 1042
Size: 1736 761 1341 1042
Fragments arranged by size:
1736 1341 1042 761
BclI T'GATC_A
Cuts at: 2400 2400
Size: 4880
BglII A'GATC_T
Cuts at: 2395 4432 2395
Size: 2037 2843
BlpI GC'TnA_GC
Cuts at: 1992 1992
Size: 4880
Bme1580I G_kGCm'C
Cuts at: 170 183 2505 2598 3654 170
Size: 13 2322 93 1056 1396
Fragments arranged by size:
2322 1396 1056 93 13
BmrI ACTGGGnnnn_n'
Cuts at: 472 2092 2173 2508 4652 472
Size: 1620 81 335 2144 700
Fragments arranged by size:
2144 1620 700 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3247 3247
Size: 4880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1078
Size: 4880
Bpu10I CC'TnA_GC
Cuts at: 1167 1167
Size: 4880
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2056 2935 3427 3725 2056
Size: 879 492 298 3211
Fragments arranged by size:
3211 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4584 4584
Size: 4880
BsaAI yAC'GTr
Cuts at: 2233 2233
Size: 4880
BsaBI GATnn'nnATC
Cuts at: 6 1679 2394 4431 6
Size: 1673 715 2037 455
Fragments arranged by size:
2037 1673 715 455
BsaHI Gr'CG_yC
Cuts at: 702 2311 2559 3261 4757 702
Size: 1609 248 702 1496 825
Fragments arranged by size:
1609 1496 825 702 248
BsaWI w'CCGG_w
Cuts at: 158 1832 2069 2590 3268 3542 3689 4100 158
Size: 1674 237 521 678 274 147 411 938
Fragments arranged by size:
1674 938 678 521 411 274 237 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1734 1764 1734
Size: 30 4850
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 736 1319 736
Size: 583 4297
BseYI C'CCAG_C
Cuts at: 869 2305 3063 3640 4136 869
Size: 1436 758 577 496 1613
Fragments arranged by size:
1613 1436 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 159 163 2422 2468 3676 104
Size: 55 4 2259 46 1208 1308
Fragments arranged by size:
2259 1308 1208 55 46 4
BsiHKAI G_wGCw'C
Cuts at: 1894 2672 2862 3654 1894
Size: 778 190 792 3120
Fragments arranged by size:
3120 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 170 183 1894 2505 2598 2672 2862 2924 3654
Size: 13 1711 611 93 74 190 62 730
Cuts at: 3654 170
Size: 1396
Fragments arranged by size:
1711 1396 730 611 190 93 74 62
13
BspEI T'CCGG_A
Cuts at: 1832 4100 1832
Size: 2268 2612
BspHI T'CATG_A
Cuts at: 4056 4056
Size: 4880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1930 2144 2446 2827 1930
Size: 214 302 381 3983
Fragments arranged by size:
3983 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3172 3226 4710 4875 50
Size: 3122 54 1484 165 55
Fragments arranged by size:
3122 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1119 2792 1119
Size: 1673 3207
BsrFI r'CCGG_y
Cuts at: 158 2878 3059 3268 158
Size: 2720 181 209 1770
Fragments arranged by size:
2720 1770 209 181
BsrGI T'GTAC_A
Cuts at: 110 110
Size: 4880
BssSI C'ACGA_G
Cuts at: 1717 3151 3509 1717
Size: 1434 358 3088
Fragments arranged by size:
3088 1434 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1821 2257 1821
Size: 436 4444
BstBI TT'CG_AA
Cuts at: 406 3241 406
Size: 2835 2045
BstEII G'GTnAC_C
Cuts at: 1456 1456
Size: 4880
BstYI r'GATC_y
Cuts at: 1 439 1616 1905 2372 2395 2730 2976 3988
Size: 438 1177 289 467 23 335 246 1012
Cuts at: 3988 4074 4086 4432 1
Size: 86 12 346 449
Fragments arranged by size:
1177 1012 467 449 438 346 335 289
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 647 647
Size: 4880
BtgI C'CryG_G
Cuts at: 347 1251 347
Size: 904 3976
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2830 3011 3174 2830
Size: 181 163 4536
Fragments arranged by size:
4536 181 163
BtsI GCAGTG_nn'
Cuts at: 1110 1472 2123 1110
Size: 362 651 3867
Fragments arranged by size:
3867 651 362
DraI TTT'AAA
Cuts at: 1879 1879
Size: 4880
DraIII CAC_nnn'GTG
Cuts at: 282 282
Size: 4880
DrdI GACnn_nn'nnGTC
Cuts at: 2586 3444 2586
Size: 858 4022
EaeI y'GGCC_r
Cuts at: 597 2465 2639 3030 3057 4402 597
Size: 1868 174 391 27 1345 1075
Fragments arranged by size:
1868 1345 1075 391 174 27
EagI C'GGCC_G
Cuts at: 2465 2465
Size: 4880
EarI CTCTTCn'nnn_
Cuts at: 184 417 674 872 1834 2903 3113 184
Size: 233 257 198 962 1069 210 1951
Fragments arranged by size:
1951 1069 962 257 233 210 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1084 3088 3398 3544 4690 1084
Size: 2004 310 146 1146 1274
Fragments arranged by size:
2004 1274 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1892 1892
Size: 4880
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 374 698 1078 1618 2705 3137 3884 4152
Size: 166 324 380 540 1087 432 747 268
Cuts at: 4152 208
Size: 936
Fragments arranged by size:
1087 936 747 540 432 380 324 268
166
EcoO109I rG'GnC_Cy
Cuts at: 180 946 2019 180
Size: 766 1073 3041
Fragments arranged by size:
3041 1073 766
EcoRI G'AATT_C
Cuts at: 1884 1884
Size: 4880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1298 2899 1298
Size: 1601 3279
FspI TGC'GCA
Cuts at: 2661 2661
Size: 4880
HaeII r_GCGC'y
Cuts at: 201 2314 2562 3584 4563 201
Size: 2113 248 1022 979 518
Fragments arranged by size:
2113 1022 979 518 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2722 2752 2754 2784 2722
Size: 30 2 30 4818
Fragments arranged by size:
4818 30 30 2
HincII GTy'rAC
Cuts at: 1919 1919
Size: 4880
KasI G'GCGC_C
Cuts at: 2310 2558 2310
Size: 248 4632
KpnI G_GTAC'C
Cuts at: 1900 1900
Size: 4880
MluI A'CGCG_T
Cuts at: 4313 4313
Size: 4880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 996 3551 3735 996
Size: 2555 184 2141
Fragments arranged by size:
2555 2141 184
MscI TGG'CCA
Cuts at: 2641 2641
Size: 4880
MslI CAynn'nnrTG
Cuts at: 2869 2996 2869
Size: 127 4753
MspA1I CmG'CkG
Cuts at: 333 1734 1991 2305 2665 3678 3923 333
Size: 1401 257 314 360 1013 245 1290
Fragments arranged by size:
1401 1290 1013 360 314 257 245
NaeI GCC'GGC
Cuts at: 3061 3061
Size: 4880
NarI GG'CG_CC
Cuts at: 2311 2559 2311
Size: 248 4632
NcoI C'CATG_G
Cuts at: 347 1251 347
Size: 904 3976
NgoMIV G'CCGG_C
Cuts at: 3059 3059
Size: 4880
NspI r_CATG'y
Cuts at: 456 1933 2433 3340 456
Size: 1477 500 907 1996
Fragments arranged by size:
1996 1477 907 500
PciI A'CATG_T
Cuts at: 3336 3336
Size: 4880
PflMI CCAn_nnn'nTGG
Cuts at: 869 869
Size: 4880
PfoI T'CCnGG_A
Cuts at: 442 442
Size: 4880
PmeI GTTT'AAAC
Cuts at: 1879 1879
Size: 4880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 4019
Size: 32 4848
PpuMI rG'GwC_Cy
Cuts at: 946 946
Size: 4880
PspOMI G'GGCC_C
Cuts at: 179 179
Size: 4880
PspXI vC'TCGA_Gb
Cuts at: 1301 1301
Size: 4880
PstI C_TGCA'G
Cuts at: 1927 2612 1927
Size: 685 4195
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4880
PvuII CAG'CTG
Cuts at: 333 1734 2305 2665 333
Size: 1401 571 360 2548
Fragments arranged by size:
2548 1401 571 360
RsrII CG'GwC_CG
Cuts at: 3075 3075
Size: 4880
SacI G_AGCT'C
Cuts at: 1894 1894
Size: 4880
SalI G'TCGA_C
Cuts at: 1917 1917
Size: 4880
SapI GCTCTTCn'nnn_
Cuts at: 872 2903 3113 872
Size: 2031 210 2639
Fragments arranged by size:
2639 2031 210
SbfI CC_TGCA'GG
Cuts at: 1927 1927
Size: 4880
ScaI AGT'ACT
Cuts at: 1425 4397 1425
Size: 2972 1908
SexAI A'CCwGG_T
Cuts at: 591 591
Size: 4880
SfcI C'TryA_G
Cuts at: 33 221 1923 2608 3601 3792 33
Size: 188 1702 685 993 191 1121
Fragments arranged by size:
1702 1121 993 685 191 188
SfoI GGC'GCC
Cuts at: 2312 2560 2312
Size: 248 4632
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
SmaI CCC'GGG
Cuts at: 178 1902 178
Size: 1724 3156
SmlI C'TyrA_G
Cuts at: 1301 2035 2950 3442 3704 1301
Size: 734 915 492 262 2477
Fragments arranged by size:
2477 915 734 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4880
SphI G_CATG'C
Cuts at: 1933 2433 1933
Size: 500 4380
SrfI GCCC'GGGC
Cuts at: 178 178
Size: 4880
StyI C'CwwG_G
Cuts at: 347 1245 1251 2014 2368 347
Size: 898 6 763 354 2859
Fragments arranged by size:
2859 898 763 354 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2463 2596 3055 3264 2463
Size: 133 459 209 4079
Fragments arranged by size:
4079 459 209 133
TatI w'GTAC_w
Cuts at: 110 712 1423 4395 110
Size: 602 711 2972 595
Fragments arranged by size:
2972 711 602 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 524 556 1734 1766 4397 4429 524
Size: 32 1178 32 2631 32 975
Fragments arranged by size:
2631 1178 975 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2677 2677
Size: 4880
XbaI T'CTAG_A
Cuts at: 1911 1911
Size: 4880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 345 2375 345
Size: 2030 2850
XhoI C'TCGA_G
Cuts at: 1301 1301
Size: 4880
XmaI C'CCGG_G
Cuts at: 176 1900 176
Size: 1724 3156
XmnI GAAnn'nnTTC
Cuts at: 1511 1856 1511
Size: 345 4535
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BssHII BstXI BstZ17I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PsrI SacII SanDI SfiI SgrAI SnaBI SspI
StuI SwaI ZraI