Restriction map for
pF1KA1332
(Circular) (Six-base) MAPSORT of: pF1KA1332.seq Check: 2463 from: 1 to: 5264
pF1KA1332 5264 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2302 2302
Size: 5264
Acc65I G'GTAC_C
Cuts at: 2280 2280
Size: 5264
AclI AA'CG_TT
Cuts at: 5104 5104
Size: 5264
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 1152 1352 1541 3089 3521 3874 4536 590
Size: 562 200 189 1548 432 353 662 1318
Fragments arranged by size:
1548 1318 662 562 432 353 200 189
AfeI AGC'GCT
Cuts at: 463 463
Size: 5264
AflIII A'CryG_T
Cuts at: 2616 4416 4697 2616
Size: 1800 281 3183
Fragments arranged by size:
3183 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2053 2053
Size: 5264
AlwNI CAG_nnn'CTG
Cuts at: 1924 2569 4007 1924
Size: 645 1438 3181
Fragments arranged by size:
3181 1438 645
ApaI G_GGCC'C
Cuts at: 1116 1116
Size: 5264
ApaLI G'TGCA_C
Cuts at: 4102 4102
Size: 5264
ApoI r'AATT_y
Cuts at: 2268 4617 4873 2268
Size: 2349 256 2659
Fragments arranged by size:
2659 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5264
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5264
AvaI C'yCGr_G
Cuts at: 953 1265 1376 2284 953
Size: 312 111 908 3933
Fragments arranged by size:
3933 908 312 111
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 325 358 325
Size: 33 5231
BamHI G'GATC_C
Cuts at: 590 862 1600 2289 590
Size: 272 738 689 3565
Fragments arranged by size:
3565 738 689 272
BanI G'GyrC_C
Cuts at: 1127 1768 2280 2694 2942 2977 1127
Size: 641 512 414 248 35 3414
Fragments arranged by size:
3414 641 512 414 248 35
BanII G_rGCy'C
Cuts at: 203 1116 1194 1353 2278 3308 203
Size: 913 78 159 925 1030 2159
Fragments arranged by size:
2159 1030 925 913 159 78
BbeI G_GCGC'C
Cuts at: 2698 2946 2698
Size: 248 5016
BbsI GAAGACnn'nnnn_
Cuts at: 1158 1847 1158
Size: 689 4575
BbvCI CC'TCA_GC
Cuts at: 221 221
Size: 5264
BciVI GTATCCnnnnn_n'
Cuts at: 1519 3162 4218 5264 1519
Size: 1643 1056 1046 1519
Fragments arranged by size:
1643 1519 1056 1046
BclI T'GATC_A
Cuts at: 2784 2784
Size: 5264
BfrBI ATG'CAT
Cuts at: 1059 1059
Size: 5264
BglI GCCn_nnn'nGGC
Cuts at: 1970 1970
Size: 5264
BglII A'GATC_T
Cuts at: 1587 1608 2779 4816 1587
Size: 21 1171 2037 2035
Fragments arranged by size:
2037 2035 1171 21
BlpI GC'TnA_GC
Cuts at: 2376 2376
Size: 5264
Bme1580I G_kGCm'C
Cuts at: 1116 1746 1773 1907 2889 2982 4106 1116
Size: 630 27 134 982 93 1124 2274
Fragments arranged by size:
2274 1124 982 630 134 93 27
BmrI ACTGGGnnnn_n'
Cuts at: 202 935 1199 2476 2557 2892 5036 202
Size: 733 264 1277 81 335 2144 430
Fragments arranged by size:
2144 1277 733 430 335 264 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1213 1293 1457 1712 3631 1213
Size: 80 164 255 1919 2846
Fragments arranged by size:
2846 1919 255 164 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 653 1229 1271 1427 1844 582
Size: 71 576 42 156 417 4002
Fragments arranged by size:
4002 576 417 156 71 42
Bpu10I CC'TnA_GC
Cuts at: 221 221
Size: 5264
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1072 2440 3319 4033 4331 922
Size: 150 1368 879 714 298 1855
Fragments arranged by size:
1855 1368 879 714 298 150
BsaI GGTCTCn'nnnn_
Cuts at: 1335 4968 1335
Size: 3633 1631
BsaAI yAC'GTr
Cuts at: 1734 2049 2617 1734
Size: 315 568 4381
Fragments arranged by size:
4381 568 315
BsaBI GATnn'nnATC
Cuts at: 6 494 1599 2778 4815 6
Size: 488 1105 1179 2037 455
Fragments arranged by size:
2037 1179 1105 488 455
BsaHI Gr'CG_yC
Cuts at: 2695 2943 3645 5141 2695
Size: 248 702 1496 2818
Fragments arranged by size:
2818 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2453 2974 3652 4063 4210 4484 2453
Size: 521 678 411 147 274 3233
Fragments arranged by size:
3233 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1866 1886 1896 1916 1866
Size: 20 10 20 5214
Fragments arranged by size:
5214 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 108 211 238 804 1232 1418 1835 108
Size: 103 27 566 428 186 417 3537
Fragments arranged by size:
3537 566 428 417 186 103 27
BseYI C'CCAG_C
Cuts at: 1328 1395 2689 3447 4112 4520 1328
Size: 67 1294 758 665 408 2072
Fragments arranged by size:
2072 1294 758 665 408 67
BsiEI CG_ry'CG
Cuts at: 104 1326 2806 2852 4082 104
Size: 1222 1480 46 1230 1286
Fragments arranged by size:
1480 1286 1230 1222 46
BsiHKAI G_wGCw'C
Cuts at: 2278 3056 3246 4106 2278
Size: 778 190 860 3436
Fragments arranged by size:
3436 860 778 190
BsmI GAATG_Cn'
Cuts at: 1059 1059
Size: 5264
BsmBI CGTCTCn'nnnn_
Cuts at: 1400 1764 1400
Size: 364 4900
Bsp1286I G_dGCh'C
Cuts at: 203 1116 1194 1353 1746 1773 1907 2278 2889
Size: 913 78 159 393 27 134 371 611
Cuts at: 2889 2982 3056 3246 3308 4106 203
Size: 93 74 190 62 798 1361
Fragments arranged by size:
1361 913 798 611 393 371 190 159
134 93 78 74 62 27
BspHI T'CATG_A
Cuts at: 386 3696 386
Size: 3310 1954
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2314 2528 2830 3211 2314
Size: 214 302 381 4367
Fragments arranged by size:
4367 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1277 3556 3610 5094 5259 50
Size: 1227 2279 54 1484 165 55
Fragments arranged by size:
2279 1484 1227 165 55 54
BsrDI GCAATG_nn'
Cuts at: 546 885 1955 3176 546
Size: 339 1070 1221 2634
Fragments arranged by size:
2634 1221 1070 339
BsrFI r'CCGG_y
Cuts at: 3262 3443 3262
Size: 181 5083
BsrGI T'GTAC_A
Cuts at: 650 1485 650
Size: 835 4429
BssSI C'ACGA_G
Cuts at: 3535 4243 3535
Size: 708 4556
BstAPI GCAn_nnn'nTGC
Cuts at: 2641 2641
Size: 5264
BstBI TT'CG_AA
Cuts at: 3625 3625
Size: 5264
BstXI CCAn_nnnn'nTGG
Cuts at: 1124 1936 1124
Size: 812 4452
BstYI r'GATC_y
Cuts at: 1 590 862 1587 1600 1608 1620 2289 2756
Size: 589 272 725 13 8 12 669 467
Cuts at: 2756 2779 3114 3360 3666 3678 3764 4816 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 725 669 589 467 449 335 306
272 246 86 23 13 12 12 8
BtgI C'CryG_G
Cuts at: 107 786 800 1649 2147 107
Size: 679 14 849 498 3224
Fragments arranged by size:
3224 849 679 498 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1816 3214 3395 3558 1816
Size: 1398 181 163 3522
Fragments arranged by size:
3522 1398 181 163
BtsI GCAGTG_nn'
Cuts at: 913 1896 2507 913
Size: 983 611 3670
Fragments arranged by size:
3670 983 611
DraI TTT'AAA
Cuts at: 2263 2263
Size: 5264
DrdI GACnn_nn'nnGTC
Cuts at: 2970 4314 2970
Size: 1344 3920
EaeI y'GGCC_r
Cuts at: 110 688 807 960 1323 1736 2849 3023 3414
Size: 578 119 153 363 413 1113 174 391
Cuts at: 3414 3441 4786 110
Size: 27 1345 588
Fragments arranged by size:
1345 1113 588 578 413 391 363 174
153 119 27
EagI C'GGCC_G
Cuts at: 1323 2849 1323
Size: 1526 3738
EarI CTCTTCn'nnn_
Cuts at: 257 1097 1473 3287 3497 257
Size: 840 376 1814 210 2024
Fragments arranged by size:
2024 1814 840 376 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3472 4214 4360 5074 3472
Size: 742 146 714 3662
Fragments arranged by size:
3662 742 714 146
EcoICRI GAG'CTC
Cuts at: 2276 2276
Size: 5264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 590 653 1152 1229 1271 1352 1427 1541
Size: 8 63 499 77 42 81 75 114
Cuts at: 1541 1844 3089 3521 3874 4536 582
Size: 303 1245 432 353 662 1310
Fragments arranged by size:
1310 1245 662 499 432 353 303 114
81 77 75 63 42 8
EcoNI CCTnn'n_nnAGG
Cuts at: 1916 1928 1916
Size: 12 5252
EcoO109I rG'GnC_Cy
Cuts at: 611 891 1112 1113 1816 1921 2403 611
Size: 280 221 1 703 105 482 3472
Fragments arranged by size:
3472 703 482 280 221 105 1
EcoRI G'AATT_C
Cuts at: 2268 2268
Size: 5264
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1405 3283 1405
Size: 1878 3386
FspI TGC'GCA
Cuts at: 3045 3045
Size: 5264
HaeII r_GCGC'y
Cuts at: 465 2698 2946 4176 4947 465
Size: 2233 248 1230 771 782
Fragments arranged by size:
2233 1230 782 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 171 203 3106 3136 3138 3168 171
Size: 32 2903 30 2 30 2267
Fragments arranged by size:
2903 2267 32 30 30 2
HincII GTy'rAC
Cuts at: 1320 2303 1320
Size: 983 4281
HpaI GTT'AAC
Cuts at: 1320 1320
Size: 5264
KasI G'GCGC_C
Cuts at: 2694 2942 2694
Size: 248 5016
KpnI G_GTAC'C
Cuts at: 2284 2284
Size: 5264
MluI A'CGCG_T
Cuts at: 4697 4697
Size: 5264
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 230 618 1759 2100 4023 4207 230
Size: 388 1141 341 1923 184 1287
Fragments arranged by size:
1923 1287 1141 388 341 184
MscI TGG'CCA
Cuts at: 112 809 962 1738 3025 112
Size: 697 153 776 1287 2351
Fragments arranged by size:
2351 1287 776 697 153
MslI CAynn'nnrTG
Cuts at: 148 2041 3253 3380 148
Size: 1893 1212 127 2032
Fragments arranged by size:
2032 1893 1212 127
MspA1I CmG'CkG
Cuts at: 536 1328 1456 2375 2689 3049 3833 4078 536
Size: 792 128 919 314 360 784 245 1722
Fragments arranged by size:
1722 919 792 784 360 314 245 128
NaeI GCC'GGC
Cuts at: 3445 3445
Size: 5264
NarI GG'CG_CC
Cuts at: 2695 2943 2695
Size: 248 5016
NcoI C'CATG_G
Cuts at: 107 786 800 1649 107
Size: 679 14 849 3722
Fragments arranged by size:
3722 849 679 14
NgoMIV G'CCGG_C
Cuts at: 3443 3443
Size: 5264
NsiI A_TGCA'T
Cuts at: 1061 1061
Size: 5264
NspI r_CATG'y
Cuts at: 482 1059 2317 2817 4420 482
Size: 577 1258 500 1603 1326
Fragments arranged by size:
1603 1326 1258 577 500
PasI CC'CwG_GG
Cuts at: 1346 1918 1346
Size: 572 4692
PciI A'CATG_T
Cuts at: 4416 4416
Size: 5264
PfoI T'CCnGG_A
Cuts at: 398 441 1443 398
Size: 43 1002 4219
Fragments arranged by size:
4219 1002 43
PmeI GTTT'AAAC
Cuts at: 2263 2263
Size: 5264
PmlI CAC'GTG
Cuts at: 1734 1734
Size: 5264
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 5232
PpuMI rG'GwC_Cy
Cuts at: 611 891 611
Size: 280 4984
PspOMI G'GGCC_C
Cuts at: 1112 1112
Size: 5264
PspXI vC'TCGA_Gb
Cuts at: 953 953
Size: 5264
PstI C_TGCA'G
Cuts at: 1833 2311 2996 1833
Size: 478 685 4101
Fragments arranged by size:
4101 685 478
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5264
PvuII CAG'CTG
Cuts at: 536 1456 2689 3049 536
Size: 920 1233 360 2751
Fragments arranged by size:
2751 1233 920 360
RsrII CG'GwC_CG
Cuts at: 3459 3459
Size: 5264
SacI G_AGCT'C
Cuts at: 2278 2278
Size: 5264
SalI G'TCGA_C
Cuts at: 2301 2301
Size: 5264
SapI GCTCTTCn'nnn_
Cuts at: 1097 1473 3287 3497 1097
Size: 376 1814 210 2864
Fragments arranged by size:
2864 1814 376 210
SbfI CC_TGCA'GG
Cuts at: 2311 2311
Size: 5264
ScaI AGT'ACT
Cuts at: 1506 2215 4781 1506
Size: 709 2566 1989
Fragments arranged by size:
2566 1989 709
SfcI C'TryA_G
Cuts at: 33 1829 2307 2992 3960 4151 33
Size: 1796 478 685 968 191 1146
Fragments arranged by size:
1796 1146 968 685 478 191
SfoI GGC'GCC
Cuts at: 2696 2944 2696
Size: 248 5016
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5264
SmaI CCC'GGG
Cuts at: 1378 2286 1378
Size: 908 4356
SmlI C'TyrA_G
Cuts at: 901 953 1087 2419 3334 4048 4310 901
Size: 52 134 1332 915 714 262 1855
Fragments arranged by size:
1855 1332 915 714 262 134 52
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5264
SphI G_CATG'C
Cuts at: 482 2317 2817 482
Size: 1835 500 2929
Fragments arranged by size:
2929 1835 500
StyI C'CwwG_G
Cuts at: 107 786 800 1069 1649 1929 2069 2398 2752
Size: 679 14 269 580 280 140 329 354
Cuts at: 2752 107
Size: 2619
Fragments arranged by size:
2619 679 580 354 329 280 269 140
14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 221 1450 2847 2980 3439 3648 221
Size: 1229 1397 133 459 209 1837
Fragments arranged by size:
1837 1397 1229 459 209 133
TatI w'GTAC_w
Cuts at: 650 1485 1504 2213 2237 4779 650
Size: 835 19 709 24 2542 1135
Fragments arranged by size:
2542 1135 835 709 24 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1142 1174 1886 1918 4781 4813 1142
Size: 32 712 32 2863 32 1593
Fragments arranged by size:
2863 1593 712 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3061 3061
Size: 5264
XbaI T'CTAG_A
Cuts at: 2295 2295
Size: 5264
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1180 1656 2759 1180
Size: 476 1103 3685
Fragments arranged by size:
3685 1103 476
XhoI C'TCGA_G
Cuts at: 953 953
Size: 5264
XmaI C'CCGG_G
Cuts at: 1376 2284 1376
Size: 908 4356
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AloI AscI
AvrII BcgI BmgBI BmtI BsgI BsiWI BspEI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII MfeI NdeI NheI NotI NruI PacI
PflMI PshAI PsiI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI