Restriction map for
pF1KA1336
(Circular) (Six-base) MAPSORT of: pF1KA1336.seq Check: 8609 from: 1 to: 6656
pF1KA1336 6656 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 576 1698 3148 3694 576
Size: 1122 1450 546 3538
Fragments arranged by size:
3538 1450 1122 546
Acc65I G'GTAC_C
Cuts at: 743 3672 743
Size: 2929 3727
AclI AA'CG_TT
Cuts at: 1333 6496 1333
Size: 5163 1493
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 2212 2237 2363 3274 4481 4913 5660 5928
Size: 1623 25 126 911 1207 432 747 268
Cuts at: 5928 589
Size: 1317
Fragments arranged by size:
1623 1317 1207 911 747 432 268 126
25
AfeI AGC'GCT
Cuts at: 2305 2305
Size: 6656
AflIII A'CryG_T
Cuts at: 3003 4008 5112 6089 3003
Size: 1005 1104 977 3570
Fragments arranged by size:
3570 1104 1005 977
AgeI A'CCGG_T
Cuts at: 5044 5044
Size: 6656
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 172 204 172
Size: 32 6624
AlwNI CAG_nnn'CTG
Cuts at: 2235 2466 2737 3623 3961 5528 2235
Size: 231 271 886 338 1567 3363
Fragments arranged by size:
3363 1567 886 338 271 231
ApaLI G'TGCA_C
Cuts at: 5426 5426
Size: 6656
ApoI r'AATT_y
Cuts at: 131 669 734 1541 3660 6009 6265 131
Size: 538 65 807 2119 2349 256 522
Fragments arranged by size:
2349 2119 807 538 522 256 65
AseI AT'TA_AT
Cuts at: 20 1502 20
Size: 1482 5174
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6656
AvaI C'yCGr_G
Cuts at: 1829 2392 3676 1829
Size: 563 1284 4809
Fragments arranged by size:
4809 1284 563
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2768 2801 2768
Size: 33 6623
BamHI G'GATC_C
Cuts at: 1374 1620 1977 2067 3681 1374
Size: 246 357 90 1614 4349
Fragments arranged by size:
4349 1614 357 246 90
BanI G'GyrC_C
Cuts at: 743 1678 2204 3672 4086 4334 4369 743
Size: 935 526 1468 414 248 35 3030
Fragments arranged by size:
3030 1468 935 526 414 248 35
BanII G_rGCy'C
Cuts at: 3670 4700 3670
Size: 1030 5626
BbeI G_GCGC'C
Cuts at: 2208 4090 4338 2208
Size: 1882 248 4526
Fragments arranged by size:
4526 1882 248
BbsI GAAGACnn'nnnn_
Cuts at: 1176 1954 3262 3290 3431 1176
Size: 778 1308 28 141 4401
Fragments arranged by size:
4401 1308 778 141 28
BciVI GTATCCnnnnn_n'
Cuts at: 177 1185 4554 5315 6656 177
Size: 1008 3369 761 1341 177
Fragments arranged by size:
3369 1341 1008 761 177
BclI T'GATC_A
Cuts at: 434 4176 434
Size: 3742 2914
BfrBI ATG'CAT
Cuts at: 1147 2164 2869 3175 1147
Size: 1017 705 306 4628
Fragments arranged by size:
4628 1017 705 306
BglII A'GATC_T
Cuts at: 494 3305 4171 6208 494
Size: 2811 866 2037 942
Fragments arranged by size:
2811 2037 942 866
BlpI GC'TnA_GC
Cuts at: 2601 3768 2601
Size: 1167 5489
Bme1580I G_kGCm'C
Cuts at: 4281 4374 5430 4281
Size: 93 1056 5507
Fragments arranged by size:
5507 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 275 3868 3949 4284 6428 275
Size: 3593 81 335 2144 503
Fragments arranged by size:
3593 2144 503 335 81
BmtI G_CTAG'C
Cuts at: 3322 3322
Size: 6656
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3327 5023 3327
Size: 1696 4960
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 734 2443 734
Size: 1709 4947
Bpu10I CC'TnA_GC
Cuts at: 121 3599 121
Size: 3478 3178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4711 5203 5501 3832
Size: 879 492 298 4987
Fragments arranged by size:
4987 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1237 1523 2120 3385 6360 1237
Size: 286 597 1265 2975 1533
Fragments arranged by size:
2975 1533 1265 597 286
BsaAI yAC'GTr
Cuts at: 765 873 3004 4009 765
Size: 108 2131 1005 3412
Fragments arranged by size:
3412 2131 1005 108
BsaBI GATnn'nnATC
Cuts at: 6 545 1289 4170 6207 6
Size: 539 744 2881 2037 455
Fragments arranged by size:
2881 2037 744 539 455
BsaHI Gr'CG_yC
Cuts at: 2205 4087 4335 5037 6533 2205
Size: 1882 248 702 1496 2328
Fragments arranged by size:
2328 1882 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3845 4366 5044 5318 5465 5876 3845
Size: 521 678 274 147 411 4625
Fragments arranged by size:
4625 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2447 2477 2447
Size: 30 6626
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 442 1318 442
Size: 876 5780
BseYI C'CCAG_C
Cuts at: 3071 4081 4839 5416 5912 3071
Size: 1010 758 577 496 3815
Fragments arranged by size:
3815 1010 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 1013 3233 3591 985
Size: 28 2220 358 4050
Fragments arranged by size:
4050 2220 358 28
BsiEI CG_ry'CG
Cuts at: 104 4198 4244 5452 104
Size: 4094 46 1208 1308
Fragments arranged by size:
4094 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2136 3670 4448 4638 5430 2136
Size: 1534 778 190 792 3362
Fragments arranged by size:
3362 1534 792 778 190
BsmI GAATG_Cn'
Cuts at: 483 2565 3072 483
Size: 2082 507 4067
Fragments arranged by size:
4067 2082 507
BsmBI CGTCTCn'nnnn_
Cuts at: 232 232
Size: 6656
Bsp1286I G_dGCh'C
Cuts at: 2136 3670 4281 4374 4448 4638 4700 5430 2136
Size: 1534 611 93 74 190 62 730 3362
Fragments arranged by size:
3362 1534 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5876 5876
Size: 6656
BspHI T'CATG_A
Cuts at: 5832 5832
Size: 6656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2354 2788 3706 3920 4222 4603 2354
Size: 434 918 214 302 381 4407
Fragments arranged by size:
4407 918 434 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4948 5002 6486 6651 50
Size: 4898 54 1484 165 55
Fragments arranged by size:
4898 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 725 1418 1943 4568 725
Size: 693 525 2625 2813
Fragments arranged by size:
2813 2625 693 525
BsrFI r'CCGG_y
Cuts at: 4654 4835 5044 4654
Size: 181 209 6266
Fragments arranged by size:
6266 209 181
BsrGI T'GTAC_A
Cuts at: 2565 2577 2565
Size: 12 6644
BssSI C'ACGA_G
Cuts at: 4927 5285 4927
Size: 358 6298
BstAPI GCAn_nnn'nTGC
Cuts at: 2737 3578 4033 2737
Size: 841 455 5360
Fragments arranged by size:
5360 841 455
BstBI TT'CG_AA
Cuts at: 5017 5017
Size: 6656
BstYI r'GATC_y
Cuts at: 1 494 1374 1620 1977 2067 2238 2409 2476
Size: 493 880 246 357 90 171 171 67
Cuts at: 2476 2848 3305 3681 4148 4171 4506 4752 5764
Size: 372 457 376 467 23 335 246 1012
Cuts at: 5764 5850 5862 6208 1
Size: 86 12 346 449
Fragments arranged by size:
1012 880 493 467 457 449 376 372
357 346 335 246 246 171 171 90
86 67 23 12
BstZ17I GTA'TAC
Cuts at: 577 577
Size: 6656
Bsu36I CC'TnA_GG
Cuts at: 1298 1982 1298
Size: 684 5972
BtgI C'CryG_G
Cuts at: 747 747
Size: 6656
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4606 4787 4950 4606
Size: 181 163 6312
Fragments arranged by size:
6312 181 163
BtsI GCAGTG_nn'
Cuts at: 166 2246 2738 3245 3579 3899 166
Size: 2080 492 507 334 320 2923
Fragments arranged by size:
2923 2080 507 492 334 320
DraI TTT'AAA
Cuts at: 3655 3655
Size: 6656
DrdI GACnn_nn'nnGTC
Cuts at: 613 1163 4362 5220 613
Size: 550 3199 858 2049
Fragments arranged by size:
3199 2049 858 550
EaeI y'GGCC_r
Cuts at: 4241 4415 4806 4833 6178 4241
Size: 174 391 27 1345 4719
Fragments arranged by size:
4719 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4241 4241
Size: 6656
EarI CTCTTCn'nnn_
Cuts at: 1881 2231 2366 2969 3269 4679 4889 1881
Size: 350 135 603 300 1410 210 3648
Fragments arranged by size:
3648 1410 603 350 300 210 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4864 5174 5320 6466 4864
Size: 310 146 1146 5054
Fragments arranged by size:
5054 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3668 3668
Size: 6656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 734 2212 2237 2363 2443 3274 4481 4913
Size: 145 1478 25 126 80 831 1207 432
Cuts at: 4913 5660 5928 589
Size: 747 268 1317
Fragments arranged by size:
1478 1317 1207 831 747 432 268 145
126 80 25
EcoNI CCTnn'n_nnAGG
Cuts at: 3598 3598
Size: 6656
EcoO109I rG'GnC_Cy
Cuts at: 267 3795 267
Size: 3528 3128
EcoRI G'AATT_C
Cuts at: 734 3660 734
Size: 2926 3730
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 637 2242 3014 4675 637
Size: 1605 772 1661 2618
Fragments arranged by size:
2618 1661 1605 772
FspI TGC'GCA
Cuts at: 3330 4437 3330
Size: 1107 5549
HaeII r_GCGC'y
Cuts at: 2208 2307 4090 4338 5360 6339 2208
Size: 99 1783 248 1022 979 2525
Fragments arranged by size:
2525 1783 1022 979 248 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 426 458 479 511 1650 1680 1682 1712 2098
Size: 32 21 32 1139 30 2 30 386
Cuts at: 2098 2100 2130 2132 3297 3329 4498 4528 4530
Size: 2 30 2 1165 32 1169 30 2
Cuts at: 4530 4560 426
Size: 30 2522
Fragments arranged by size:
2522 1169 1165 1139 386 32 32 32
30 30 30 30 30 21 2 2
2 2
HincII GTy'rAC
Cuts at: 365 2758 3695 365
Size: 2393 937 3326
Fragments arranged by size:
3326 2393 937
HindIII A'AGCT_T
Cuts at: 3365 3365
Size: 6656
KasI G'GCGC_C
Cuts at: 2204 4086 4334 2204
Size: 1882 248 4526
Fragments arranged by size:
4526 1882 248
KpnI G_GTAC'C
Cuts at: 747 3676 747
Size: 2929 3727
MluI A'CGCG_T
Cuts at: 6089 6089
Size: 6656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1842 2130 2694 5327 5511 1842
Size: 288 564 2633 184 2987
Fragments arranged by size:
2987 2633 564 288 184
MscI TGG'CCA
Cuts at: 4417 4417
Size: 6656
MslI CAynn'nnrTG
Cuts at: 833 865 1553 4645 4772 833
Size: 32 688 3092 127 2717
Fragments arranged by size:
3092 2717 688 127 32
MspA1I CmG'CkG
Cuts at: 3767 4081 4441 5454 5699 3767
Size: 314 360 1013 245 4724
Fragments arranged by size:
4724 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4837 4837
Size: 6656
NarI GG'CG_CC
Cuts at: 2205 4087 4335 2205
Size: 1882 248 4526
Fragments arranged by size:
4526 1882 248
NcoI C'CATG_G
Cuts at: 747 747
Size: 6656
NgoMIV G'CCGG_C
Cuts at: 4835 4835
Size: 6656
NheI G'CTAG_C
Cuts at: 3318 3318
Size: 6656
NsiI A_TGCA'T
Cuts at: 1149 2166 2871 3177 1149
Size: 1017 705 306 4628
Fragments arranged by size:
4628 1017 705 306
NspI r_CATG'y
Cuts at: 200 870 3335 3527 3709 4209 5116 200
Size: 670 2465 192 182 500 907 1740
Fragments arranged by size:
2465 1740 907 670 500 192 182
PciI A'CATG_T
Cuts at: 5112 5112
Size: 6656
PfoI T'CCnGG_A
Cuts at: 169 1011 2501 169
Size: 842 1490 4324
Fragments arranged by size:
4324 1490 842
PmeI GTTT'AAAC
Cuts at: 3655 3655
Size: 6656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5795 5827 5795
Size: 32 6624
PsiI TTA'TAA
Cuts at: 1112 1112
Size: 6656
PspXI vC'TCGA_Gb
Cuts at: 1829 1829
Size: 6656
PstI C_TGCA'G
Cuts at: 161 2253 3703 4388 161
Size: 2092 1450 685 2429
Fragments arranged by size:
2429 2092 1450 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6656
PvuII CAG'CTG
Cuts at: 4081 4441 4081
Size: 360 6296
RsrII CG'GwC_CG
Cuts at: 4851 4851
Size: 6656
SacI G_AGCT'C
Cuts at: 3670 3670
Size: 6656
SalI G'TCGA_C
Cuts at: 3693 3693
Size: 6656
SapI GCTCTTCn'nnn_
Cuts at: 2231 3269 4679 4889 2231
Size: 1038 1410 210 3998
Fragments arranged by size:
3998 1410 1038 210
SbfI CC_TGCA'GG
Cuts at: 3703 3703
Size: 6656
ScaI AGT'ACT
Cuts at: 2456 6173 2456
Size: 3717 2939
SfcI C'TryA_G
Cuts at: 33 157 1136 2249 3615 3699 4384 5377 5568
Size: 124 979 1113 1366 84 685 993 191
Cuts at: 5568 33
Size: 1121
Fragments arranged by size:
1366 1121 1113 993 979 685 191 124
84
SfoI GGC'GCC
Cuts at: 2206 4088 4336 2206
Size: 1882 248 4526
Fragments arranged by size:
4526 1882 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6656
SmaI CCC'GGG
Cuts at: 3678 3678
Size: 6656
SmlI C'TyrA_G
Cuts at: 1829 2392 3811 4726 5218 5480 1829
Size: 563 1419 915 492 262 3005
Fragments arranged by size:
3005 1419 915 563 492 262
SnaBI TAC'GTA
Cuts at: 873 873
Size: 6656
SpeI A'CTAG_T
Cuts at: 67 1341 67
Size: 1274 5382
SphI G_CATG'C
Cuts at: 200 3335 3709 4209 200
Size: 3135 374 500 2647
Fragments arranged by size:
3135 2647 500 374
SspI AAT'ATT
Cuts at: 1174 1726 2572 1174
Size: 552 846 5258
Fragments arranged by size:
5258 846 552
StyI C'CwwG_G
Cuts at: 747 1370 2283 3116 3790 4144 747
Size: 623 913 833 674 354 3259
Fragments arranged by size:
3259 913 833 674 623 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2102 4239 4372 4831 5040 2102
Size: 2137 133 459 209 3718
Fragments arranged by size:
3718 2137 459 209 133
TatI w'GTAC_w
Cuts at: 1338 2454 2565 2577 3021 6171 1338
Size: 1116 111 12 444 3150 1823
Fragments arranged by size:
3150 1823 1116 444 111 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1605 1637 1891 1923 3558 3590 6173 6205 1605
Size: 32 254 32 1635 32 2583 32 2056
Fragments arranged by size:
2583 2056 1635 254 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4453 4453
Size: 6656
XbaI T'CTAG_A
Cuts at: 3687 3687
Size: 6656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3114 4151 3114
Size: 1037 5619
XhoI C'TCGA_G
Cuts at: 1829 2392 1829
Size: 563 6093
XmaI C'CCGG_G
Cuts at: 3676 3676
Size: 6656
XmnI GAAnn'nnTTC
Cuts at: 1991 3096 3140 1991
Size: 1105 44 5507
Fragments arranged by size:
5507 1105 44
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PciI PfoI PmeI
PpiI PsiI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BbvCI BcgI BglI BmgBI BsiWI BssHII BstEII
BstXI ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NotI NruI PacI PasI PflMI PmlI
PpuMI PshAI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI StuI SwaI ZraI