Restriction map for
pF1KA1339
(Circular) (Six-base) MAPSORT of: pF1KA1339.seq Check: 8256 from: 1 to: 5039
pF1KA1339 5039 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 221 1119 1312 1691 221
Size: 898 193 379 3569
Fragments arranged by size:
3569 898 379 193
AatII G_ACGT'C
Cuts at: 1041 1041
Size: 5039
AccI GT'mk_AC
Cuts at: 1015 2077 1015
Size: 1062 3977
Acc65I G'GTAC_C
Cuts at: 2055 2055
Size: 5039
AclI AA'CG_TT
Cuts at: 4879 4879
Size: 5039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 926 1079 1482 1707 2000 2864 3296 3649 4311
Size: 153 403 225 293 864 432 353 662
Cuts at: 4311 926
Size: 1654
Fragments arranged by size:
1654 864 662 432 403 353 293 225
153
AfeI AGC'GCT
Cuts at: 1411 1411
Size: 5039
AflIII A'CryG_T
Cuts at: 860 1172 2391 4191 4472 860
Size: 312 1219 1800 281 1427
Fragments arranged by size:
1800 1427 1219 312 281
AleI CACnn'nnGTG
Cuts at: 1148 1148
Size: 5039
AlwNI CAG_nnn'CTG
Cuts at: 1048 2344 3782 1048
Size: 1296 1438 2305
Fragments arranged by size:
2305 1438 1296
ApaI G_GGCC'C
Cuts at: 837 837
Size: 5039
ApaLI G'TGCA_C
Cuts at: 3877 3877
Size: 5039
ApoI r'AATT_y
Cuts at: 2043 4392 4648 2043
Size: 2349 256 2434
Fragments arranged by size:
2434 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5039
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5039
AvaI C'yCGr_G
Cuts at: 2059 2059
Size: 5039
BamHI G'GATC_C
Cuts at: 485 2064 485
Size: 1579 3460
BanI G'GyrC_C
Cuts at: 528 2055 2469 2717 2752 528
Size: 1527 414 248 35 2815
Fragments arranged by size:
2815 1527 414 248 35
BanII G_rGCy'C
Cuts at: 837 1452 2053 3083 837
Size: 615 601 1030 2793
Fragments arranged by size:
2793 1030 615 601
BbeI G_GCGC'C
Cuts at: 2473 2721 2473
Size: 248 4791
BbvCI CC'TCA_GC
Cuts at: 113 1261 113
Size: 1148 3891
BciVI GTATCCnnnnn_n'
Cuts at: 2937 3993 5039 2937
Size: 1056 1046 2937
Fragments arranged by size:
2937 1056 1046
BclI T'GATC_A
Cuts at: 2559 2559
Size: 5039
BglI GCCn_nnn'nGGC
Cuts at: 259 259
Size: 5039
BglII A'GATC_T
Cuts at: 2554 4591 2554
Size: 2037 3002
BlpI GC'TnA_GC
Cuts at: 2151 2151
Size: 5039
Bme1580I G_kGCm'C
Cuts at: 837 934 1227 1234 1482 2664 2757 3881 837
Size: 97 293 7 248 1182 93 1124 1995
Fragments arranged by size:
1995 1182 1124 293 248 97 93 7
BmgBI CAC'GTC
Cuts at: 861 861
Size: 5039
BmrI ACTGGGnnnn_n'
Cuts at: 755 1221 1476 1955 2251 2332 2667 4811 755
Size: 466 255 479 296 81 335 2144 983
Fragments arranged by size:
2144 983 479 466 335 296 255 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3406 3406
Size: 5039
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 466 485 554 1100 403
Size: 63 19 69 546 4342
Fragments arranged by size:
4342 546 69 63 19
Bpu10I CC'TnA_GC
Cuts at: 113 1235 1261 113
Size: 1122 26 3891
Fragments arranged by size:
3891 1122 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1837 2215 3094 3808 4106 1837
Size: 378 879 714 298 2770
Fragments arranged by size:
2770 879 714 378 298
BsaI GGTCTCn'nnnn_
Cuts at: 1984 4743 1984
Size: 2759 2280
BsaAI yAC'GTr
Cuts at: 2392 2392
Size: 5039
BsaBI GATnn'nnATC
Cuts at: 6 2553 4590 6
Size: 2547 2037 455
Fragments arranged by size:
2547 2037 455
BsaHI Gr'CG_yC
Cuts at: 1038 2470 2718 3420 4916 1038
Size: 1432 248 702 1496 1161
Fragments arranged by size:
1496 1432 1161 702 248
BsaWI w'CCGG_w
Cuts at: 840 1442 2228 2749 3427 3838 3985 4259 840
Size: 602 786 521 678 411 147 274 1620
Fragments arranged by size:
1620 786 678 602 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 376 406 1798 1828 2012 2018 2042 2048 376
Size: 30 1392 30 184 6 24 6 3367
Fragments arranged by size:
3367 1392 184 30 30 24 6 6
BseYI C'CCAG_C
Cuts at: 192 402 2464 3222 3887 4295 192
Size: 210 2062 758 665 408 936
Fragments arranged by size:
2062 936 758 665 408 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1629 1687 277
Size: 1352 58 3629
Fragments arranged by size:
3629 1352 58
BsiEI CG_ry'CG
Cuts at: 104 2581 2627 3857 104
Size: 2477 46 1230 1286
Fragments arranged by size:
2477 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1211 1452 1682 2053 2831 3021 3881 1211
Size: 241 230 371 778 190 860 2369
Fragments arranged by size:
2369 860 778 371 241 230 190
Bsp1286I G_dGCh'C
Cuts at: 837 934 1211 1227 1234 1452 1482 1682 2053
Size: 97 277 16 7 218 30 200 371
Cuts at: 2053 2664 2757 2831 3021 3083 3881 837
Size: 611 93 74 190 62 798 1995
Fragments arranged by size:
1995 798 611 371 277 218 200 190
97 93 74 62 30 16 7
BspHI T'CATG_A
Cuts at: 620 3471 620
Size: 2851 2188
BspMI ACCTGCnnnn'nnnn_
Cuts at: 221 470 1061 1119 1312 1691 2089 2303 2605
Size: 249 591 58 193 379 398 214 302
Cuts at: 2605 2986 221
Size: 381 2274
Fragments arranged by size:
2274 591 398 381 379 302 249 214
193 58
BsrBI CCG'CTC
Cuts at: 50 1716 1800 3331 3385 4869 5034 50
Size: 1666 84 1531 54 1484 165 55
Fragments arranged by size:
1666 1531 1484 165 84 55 54
BsrDI GCAATG_nn'
Cuts at: 2951 2951
Size: 5039
BsrFI r'CCGG_y
Cuts at: 290 1694 3037 3218 290
Size: 1404 1343 181 2111
Fragments arranged by size:
2111 1404 1343 181
BsrGI T'GTAC_A
Cuts at: 863 863
Size: 5039
BssSI C'ACGA_G
Cuts at: 3310 4018 3310
Size: 708 4331
BstAPI GCAn_nnn'nTGC
Cuts at: 979 1652 2416 979
Size: 673 764 3602
Fragments arranged by size:
3602 764 673
BstBI TT'CG_AA
Cuts at: 3400 3400
Size: 5039
BstXI CCAn_nnnn'nTGG
Cuts at: 1150 2005 1150
Size: 855 4184
BstYI r'GATC_y
Cuts at: 1 281 485 919 2064 2531 2554 2889 3135
Size: 280 204 434 1145 467 23 335 246
Cuts at: 3135 3441 3453 3539 4591 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1145 1052 467 449 434 335 306 280
246 204 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 824 935 824
Size: 111 4928
BtgI C'CryG_G
Cuts at: 107 650 107
Size: 543 4496
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2989 3170 3333 2989
Size: 181 163 4695
Fragments arranged by size:
4695 181 163
BtsI GCAGTG_nn'
Cuts at: 2282 2282
Size: 5039
DraI TTT'AAA
Cuts at: 2038 2038
Size: 5039
DraIII CAC_nnn'GTG
Cuts at: 1315 1315
Size: 5039
DrdI GACnn_nn'nnGTC
Cuts at: 2745 4089 2745
Size: 1344 3695
EaeI y'GGCC_r
Cuts at: 248 1587 2624 2798 3189 3216 4561 248
Size: 1339 1037 174 391 27 1345 726
Fragments arranged by size:
1345 1339 1037 726 391 174 27
EagI C'GGCC_G
Cuts at: 2624 2624
Size: 5039
EarI CTCTTCn'nnn_
Cuts at: 185 761 812 881 960 3062 3272 185
Size: 576 51 69 79 2102 210 1952
Fragments arranged by size:
2102 1952 576 210 79 69 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1270 1594 3247 3989 4135 4849 1270
Size: 324 1653 742 146 714 1460
Fragments arranged by size:
1653 1460 742 714 324 146
EcoICRI GAG'CTC
Cuts at: 1450 2051 1450
Size: 601 4438
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 466 485 554 926 1079 1100 1482 1707
Size: 63 19 69 372 153 21 382 225
Cuts at: 1707 2000 2864 3296 3649 4311 403
Size: 293 864 432 353 662 1131
Fragments arranged by size:
1131 864 662 432 382 372 353 293
225 153 69 63 21 19
EcoNI CCTnn'n_nnAGG
Cuts at: 415 415
Size: 5039
EcoO109I rG'GnC_Cy
Cuts at: 833 834 1466 1933 2178 833
Size: 1 632 467 245 3694
Fragments arranged by size:
3694 632 467 245 1
EcoRI G'AATT_C
Cuts at: 2043 2043
Size: 5039
EcoRV GAT'ATC
Cuts at: 519 519
Size: 5039
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 5039
FspI TGC'GCA
Cuts at: 469 1672 2820 469
Size: 1203 1148 2688
Fragments arranged by size:
2688 1203 1148
HaeII r_GCGC'y
Cuts at: 1413 1551 2473 2721 3951 4722 1413
Size: 138 922 248 1230 771 1730
Fragments arranged by size:
1730 1230 922 771 248 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 535 567 667 699 781 813 1071 1103 1314
Size: 32 100 32 82 32 258 32 211
Cuts at: 1314 1346 2881 2911 2913 2943 535
Size: 32 1535 30 2 30 2631
Fragments arranged by size:
2631 1535 258 211 100 82 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1974 2078 1974
Size: 104 4935
KasI G'GCGC_C
Cuts at: 2469 2717 2469
Size: 248 4791
KpnI G_GTAC'C
Cuts at: 2059 2059
Size: 5039
MluI A'CGCG_T
Cuts at: 4472 4472
Size: 5039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 295 3798 3982 295
Size: 3503 184 1352
Fragments arranged by size:
3503 1352 184
MscI TGG'CCA
Cuts at: 2800 2800
Size: 5039
MslI CAynn'nnrTG
Cuts at: 138 649 1148 3028 3155 138
Size: 511 499 1880 127 2022
Fragments arranged by size:
2022 1880 511 499 127
MspA1I CmG'CkG
Cuts at: 347 381 1475 1530 2150 2464 2824 3608 3853
Size: 34 1094 55 620 314 360 784 245
Cuts at: 3853 347
Size: 1533
Fragments arranged by size:
1533 1094 784 620 360 314 245 55
34
NaeI GCC'GGC
Cuts at: 292 3220 292
Size: 2928 2111
NarI GG'CG_CC
Cuts at: 2470 2718 2470
Size: 248 4791
NcoI C'CATG_G
Cuts at: 107 650 107
Size: 543 4496
NgoMIV G'CCGG_C
Cuts at: 290 3218 290
Size: 2928 2111
NspI r_CATG'y
Cuts at: 1176 2092 2592 4195 1176
Size: 916 500 1603 2020
Fragments arranged by size:
2020 1603 916 500
PciI A'CATG_T
Cuts at: 1172 4191 1172
Size: 3019 2020
PflMI CCAn_nnn'nTGG
Cuts at: 2000 2000
Size: 5039
PmeI GTTT'AAAC
Cuts at: 2038 2038
Size: 5039
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1117 1149 3485 3517 1117
Size: 32 2336 32 2639
Fragments arranged by size:
2639 2336 32 32
PpuMI rG'GwC_Cy
Cuts at: 1933 1933
Size: 5039
PspOMI G'GGCC_C
Cuts at: 833 833
Size: 5039
PstI C_TGCA'G
Cuts at: 170 218 419 437 1657 2086 2771 170
Size: 48 201 18 1220 429 685 2438
Fragments arranged by size:
2438 1220 685 429 201 48 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5039
PvuII CAG'CTG
Cuts at: 347 381 2464 2824 347
Size: 34 2083 360 2562
Fragments arranged by size:
2562 2083 360 34
RsrII CG'GwC_CG
Cuts at: 3234 3234
Size: 5039
SacI G_AGCT'C
Cuts at: 1452 2053 1452
Size: 601 4438
SalI G'TCGA_C
Cuts at: 2076 2076
Size: 5039
SapI GCTCTTCn'nnn_
Cuts at: 761 812 881 3062 3272 761
Size: 51 69 2181 210 2528
Fragments arranged by size:
2528 2181 210 69 51
SbfI CC_TGCA'GG
Cuts at: 2086 2086
Size: 5039
ScaI AGT'ACT
Cuts at: 4556 4556
Size: 5039
SexAI A'CCwGG_T
Cuts at: 1929 1929
Size: 5039
SfcI C'TryA_G
Cuts at: 33 166 214 263 415 433 1653 2082 2767
Size: 133 48 49 152 18 1220 429 685
Cuts at: 2767 3735 3926 33
Size: 968 191 1146
Fragments arranged by size:
1220 1146 968 685 429 191 152 133
49 48 18
SfoI GGC'GCC
Cuts at: 2471 2719 2471
Size: 248 4791
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5039
SgrAI Cr'CCGG_yG
Cuts at: 1694 1694
Size: 5039
SmaI CCC'GGG
Cuts at: 2061 2061
Size: 5039
SmlI C'TyrA_G
Cuts at: 1852 2194 3109 3823 4085 1852
Size: 342 915 714 262 2806
Fragments arranged by size:
2806 915 714 342 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5039
SphI G_CATG'C
Cuts at: 2092 2592 2092
Size: 500 4539
StuI AGG'CCT
Cuts at: 911 911
Size: 5039
StyI C'CwwG_G
Cuts at: 107 650 848 2173 2527 107
Size: 543 198 1325 354 2619
Fragments arranged by size:
2619 1325 543 354 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1349 1935 1957 2622 2755 3214 3423 1349
Size: 586 22 665 133 459 209 2965
Fragments arranged by size:
2965 665 586 459 209 133 22
TatI w'GTAC_w
Cuts at: 863 4554 863
Size: 3691 1348
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 136 168 376 408 1365 1397 4556 4588 136
Size: 32 208 32 957 32 3159 32 587
Fragments arranged by size:
3159 957 587 208 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2836 2836
Size: 5039
XbaI T'CTAG_A
Cuts at: 1986 2070 1986
Size: 84 4955
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2534 2534
Size: 5039
XmaI C'CCGG_G
Cuts at: 2059 2059
Size: 5039
ZraI GAC'GTC
Cuts at: 1039 1039
Size: 5039
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AlfI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BseRI BsiWI BsmI BsmBI
BspEI BssHII BstEII BstZ17I ClaI CspCI FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SnaBI SrfI SspI SwaI XhoI
XmnI