Restriction map for
pF1KA1342
(Circular) (Six-base) MAPSORT of: pF1KA1342.seq Check: 8519 from: 1 to: 4388
pF1KA1342 4388 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1426 1426
Size: 4388
Acc65I G'GTAC_C
Cuts at: 1404 1404
Size: 4388
AclI AA'CG_TT
Cuts at: 4228 4228
Size: 4388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 530 929 947 2213 2645 3392 3660 153
Size: 377 399 18 1266 432 747 268 881
Fragments arranged by size:
1266 881 747 432 399 377 268 18
AflII C'TTAA_G
Cuts at: 277 277
Size: 4388
AflIII A'CryG_T
Cuts at: 1740 2844 3821 1740
Size: 1104 977 2307
Fragments arranged by size:
2307 1104 977
AgeI A'CCGG_T
Cuts at: 2776 2776
Size: 4388
AleI CACnn'nnGTG
Cuts at: 157 157
Size: 4388
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 231 231
Size: 4388
AlwNI CAG_nnn'CTG
Cuts at: 1693 3260 1693
Size: 1567 2821
ApaLI G'TGCA_C
Cuts at: 3158 3158
Size: 4388
ApoI r'AATT_y
Cuts at: 136 375 1225 1392 3741 3997 136
Size: 239 850 167 2349 256 527
Fragments arranged by size:
2349 850 527 256 239 167
AseI AT'TA_AT
Cuts at: 20 20
Size: 4388
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
AvaI C'yCGr_G
Cuts at: 891 1408 891
Size: 517 3871
BamHI G'GATC_C
Cuts at: 753 1413 753
Size: 660 3728
BanI G'GyrC_C
Cuts at: 1404 1818 2066 2101 1404
Size: 414 248 35 3691
Fragments arranged by size:
3691 414 248 35
BanII G_rGCy'C
Cuts at: 1402 2432 1402
Size: 1030 3358
BbeI G_GCGC'C
Cuts at: 1822 2070 1822
Size: 248 4140
BbsI GAAGACnn'nnnn_
Cuts at: 180 953 1138 180
Size: 773 185 3430
Fragments arranged by size:
3430 773 185
BciVI GTATCCnnnnn_n'
Cuts at: 782 2286 3047 4388 782
Size: 1504 761 1341 782
Fragments arranged by size:
1504 1341 782 761
BclI T'GATC_A
Cuts at: 1908 1908
Size: 4388
BglII A'GATC_T
Cuts at: 405 1328 1903 3940 405
Size: 923 575 2037 853
Fragments arranged by size:
2037 923 853 575
BlpI GC'TnA_GC
Cuts at: 1500 1500
Size: 4388
Bme1580I G_kGCm'C
Cuts at: 2013 2106 3162 2013
Size: 93 1056 3239
Fragments arranged by size:
3239 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1600 1681 2016 4160 1600
Size: 81 335 2144 1828
Fragments arranged by size:
2144 1828 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 4388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 481
Size: 4388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1564 2443 2935 3233 1564
Size: 879 492 298 2719
Fragments arranged by size:
2719 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 763 4092 763
Size: 3329 1059
BsaAI yAC'GTr
Cuts at: 1368 1741 1368
Size: 373 4015
BsaBI GATnn'nnATC
Cuts at: 6 1902 3939 6
Size: 1896 2037 455
Fragments arranged by size:
2037 1896 455
BsaHI Gr'CG_yC
Cuts at: 1819 2067 2769 4265 1819
Size: 248 702 1496 1942
Fragments arranged by size:
1942 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1066 1577 2098 2776 3050 3197 3608 1066
Size: 511 521 678 274 147 411 1846
Fragments arranged by size:
1846 678 521 511 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 242 242
Size: 4388
BseYI C'CCAG_C
Cuts at: 567 1813 2571 3148 3644 567
Size: 1246 758 577 496 1311
Fragments arranged by size:
1311 1246 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1310 1310
Size: 4388
BsiEI CG_ry'CG
Cuts at: 104 1930 1976 3184 104
Size: 1826 46 1208 1308
Fragments arranged by size:
1826 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1319 1373 1402 2180 2370 3162 1319
Size: 54 29 778 190 792 2545
Fragments arranged by size:
2545 792 778 190 54 29
BsmI GAATG_Cn'
Cuts at: 471 471
Size: 4388
Bsp1286I G_dGCh'C
Cuts at: 1319 1373 1402 2013 2106 2180 2370 2432 3162
Size: 54 29 611 93 74 190 62 730
Cuts at: 3162 1319
Size: 2545
Fragments arranged by size:
2545 730 611 190 93 74 62 54
29
BspEI T'CCGG_A
Cuts at: 1066 3608 1066
Size: 2542 1846
BspHI T'CATG_A
Cuts at: 3564 3564
Size: 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1438 1652 1954 2335 1438
Size: 214 302 381 3491
Fragments arranged by size:
3491 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2680 2734 4218 4383 50
Size: 2630 54 1484 165 55
Fragments arranged by size:
2630 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 379 2300 379
Size: 1921 2467
BsrFI r'CCGG_y
Cuts at: 2386 2567 2776 2386
Size: 181 209 3998
Fragments arranged by size:
3998 209 181
BssSI C'ACGA_G
Cuts at: 2659 3017 2659
Size: 358 4030
BstAPI GCAn_nnn'nTGC
Cuts at: 1765 1765
Size: 4388
BstBI TT'CG_AA
Cuts at: 2749 2749
Size: 4388
BstXI CCAn_nnnn'nTGG
Cuts at: 159 159
Size: 4388
BstYI r'GATC_y
Cuts at: 1 164 212 390 405 753 1077 1328 1413
Size: 163 48 178 15 348 324 251 85
Cuts at: 1413 1880 1903 2238 2484 3496 3582 3594 3940
Size: 467 23 335 246 1012 86 12 346
Cuts at: 3940 1
Size: 449
Fragments arranged by size:
1012 467 449 348 346 335 324 251
246 178 163 86 85 48 23 15
12
BtgI C'CryG_G
Cuts at: 107 639 653 107
Size: 532 14 3842
Fragments arranged by size:
3842 532 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2338 2519 2682 2338
Size: 181 163 4044
Fragments arranged by size:
4044 181 163
BtsI GCAGTG_nn'
Cuts at: 1169 1631 1169
Size: 462 3926
DraI TTT'AAA
Cuts at: 524 1387 524
Size: 863 3525
DrdI GACnn_nn'nnGTC
Cuts at: 2094 2952 2094
Size: 858 3530
EaeI y'GGCC_r
Cuts at: 1973 2147 2538 2565 3910 1973
Size: 174 391 27 1345 2451
Fragments arranged by size:
2451 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1973 1973
Size: 4388
EarI CTCTTCn'nnn_
Cuts at: 545 583 2411 2621 545
Size: 38 1828 210 2312
Fragments arranged by size:
2312 1828 210 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2596 2906 3052 4198 2596
Size: 310 146 1146 2786
Fragments arranged by size:
2786 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1400 1400
Size: 4388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 481 530 929 947 2213 2645 3392 3660
Size: 328 49 399 18 1266 432 747 268
Cuts at: 3660 153
Size: 881
Fragments arranged by size:
1266 881 747 432 399 328 268 49
18
EcoO109I rG'GnC_Cy
Cuts at: 1203 1253 1527 1203
Size: 50 274 4064
Fragments arranged by size:
4064 274 50
EcoRI G'AATT_C
Cuts at: 375 1392 375
Size: 1017 3371
EcoRV GAT'ATC
Cuts at: 864 864
Size: 4388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4388
FspI TGC'GCA
Cuts at: 2169 2169
Size: 4388
HaeII r_GCGC'y
Cuts at: 1822 2070 3092 4071 1822
Size: 248 1022 979 2139
Fragments arranged by size:
2139 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 204 236 649 681 848 880 923 955 2230
Size: 32 413 32 167 32 43 32 1275
Cuts at: 2230 2260 2262 2292 204
Size: 30 2 30 2300
Fragments arranged by size:
2300 1275 413 167 43 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1427 1427
Size: 4388
KasI G'GCGC_C
Cuts at: 1818 2066 1818
Size: 248 4140
KpnI G_GTAC'C
Cuts at: 1408 1408
Size: 4388
MluI A'CGCG_T
Cuts at: 3821 3821
Size: 4388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3059 3243 3059
Size: 184 4204
MscI TGG'CCA
Cuts at: 2149 2149
Size: 4388
MslI CAynn'nnrTG
Cuts at: 157 2377 2504 157
Size: 2220 127 2041
Fragments arranged by size:
2220 2041 127
MspA1I CmG'CkG
Cuts at: 364 1499 1813 2173 3186 3431 364
Size: 1135 314 360 1013 245 1321
Fragments arranged by size:
1321 1135 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2569 2569
Size: 4388
NarI GG'CG_CC
Cuts at: 1819 2067 1819
Size: 248 4140
NcoI C'CATG_G
Cuts at: 107 653 107
Size: 546 3842
NgoMIV G'CCGG_C
Cuts at: 2567 2567
Size: 4388
NspI r_CATG'y
Cuts at: 274 1441 1941 2848 274
Size: 1167 500 907 1814
Fragments arranged by size:
1814 1167 907 500
PciI A'CATG_T
Cuts at: 2844 2844
Size: 4388
PflMI CCAn_nnn'nTGG
Cuts at: 813 813
Size: 4388
PmeI GTTT'AAAC
Cuts at: 1387 1387
Size: 4388
PmlI CAC'GTG
Cuts at: 1368 1368
Size: 4388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 4356
PpuMI rG'GwC_Cy
Cuts at: 1253 1253
Size: 4388
PstI C_TGCA'G
Cuts at: 1435 2120 1435
Size: 685 3703
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4388
PvuII CAG'CTG
Cuts at: 364 1813 2173 364
Size: 1449 360 2579
Fragments arranged by size:
2579 1449 360
RsrII CG'GwC_CG
Cuts at: 2583 2583
Size: 4388
SacI G_AGCT'C
Cuts at: 1402 1402
Size: 4388
SalI G'TCGA_C
Cuts at: 1425 1425
Size: 4388
SanDI GG'GwC_CC
Cuts at: 1253 1253
Size: 4388
SapI GCTCTTCn'nnn_
Cuts at: 2411 2621 2411
Size: 210 4178
SbfI CC_TGCA'GG
Cuts at: 1435 1435
Size: 4388
ScaI AGT'ACT
Cuts at: 3905 3905
Size: 4388
SfcI C'TryA_G
Cuts at: 33 1431 2116 3109 3300 33
Size: 1398 685 993 191 1121
Fragments arranged by size:
1398 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1820 2068 1820
Size: 248 4140
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
SmaI CCC'GGG
Cuts at: 1410 1410
Size: 4388
SmlI C'TyrA_G
Cuts at: 277 1543 2458 2950 3212 277
Size: 1266 915 492 262 1453
Fragments arranged by size:
1453 1266 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4388
SphI G_CATG'C
Cuts at: 1441 1941 1441
Size: 500 3888
StyI C'CwwG_G
Cuts at: 107 653 749 1522 1876 107
Size: 546 96 773 354 2619
Fragments arranged by size:
2619 773 546 354 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 814 1276 1971 2104 2563 2772 814
Size: 462 695 133 459 209 2430
Fragments arranged by size:
2430 695 462 459 209 133
TatI w'GTAC_w
Cuts at: 3903 3903
Size: 4388
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 156 188 3905 3937 156
Size: 32 3717 32 607
Fragments arranged by size:
3717 607 32 32
Tth111I GACn'n_nGTC
Cuts at: 2185 2185
Size: 4388
XbaI T'CTAG_A
Cuts at: 1419 1419
Size: 4388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1883 1883
Size: 4388
XmaI C'CCGG_G
Cuts at: 1408 1408
Size: 4388
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI