Restriction map for
pF1KA1343
(Circular) (Six-base) MAPSORT of: pF1KA1343.seq Check: 6491 from: 1 to: 4598
pF1KA1343 4598 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1636 1636
Size: 4598
Acc65I G'GTAC_C
Cuts at: 1614 1614
Size: 4598
AclI AA'CG_TT
Cuts at: 4438 4438
Size: 4598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2855 3208 3870 2423
Size: 432 353 662 3151
Fragments arranged by size:
3151 662 432 353
AflIII A'CryG_T
Cuts at: 1950 3750 4031 1950
Size: 1800 281 2517
Fragments arranged by size:
2517 1800 281
AgeI A'CCGG_T
Cuts at: 1515 1515
Size: 4598
AhdI GACnn_n'nnGTC
Cuts at: 1280 1280
Size: 4598
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 656 688 656
Size: 32 4566
AlwNI CAG_nnn'CTG
Cuts at: 1903 3341 1903
Size: 1438 3160
ApaLI G'TGCA_C
Cuts at: 899 3436 899
Size: 2537 2061
ApoI r'AATT_y
Cuts at: 145 934 1562 1602 3951 4207 145
Size: 789 628 40 2349 256 536
Fragments arranged by size:
2349 789 628 536 256 40
AseI AT'TA_AT
Cuts at: 20 965 20
Size: 945 3653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
AvaI C'yCGr_G
Cuts at: 1618 1618
Size: 4598
BamHI G'GATC_C
Cuts at: 137 1623 137
Size: 1486 3112
BanI G'GyrC_C
Cuts at: 1614 2028 2276 2311 1614
Size: 414 248 35 3901
Fragments arranged by size:
3901 414 248 35
BanII G_rGCy'C
Cuts at: 1612 2642 1612
Size: 1030 3568
BbeI G_GCGC'C
Cuts at: 2032 2280 2032
Size: 248 4350
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1405 1439 1405
Size: 34 4564
BciVI GTATCCnnnnn_n'
Cuts at: 229 2496 3552 4598 229
Size: 2267 1056 1046 229
Fragments arranged by size:
2267 1056 1046 229
BclI T'GATC_A
Cuts at: 2118 2118
Size: 4598
BglII A'GATC_T
Cuts at: 2113 4150 2113
Size: 2037 2561
BlpI GC'TnA_GC
Cuts at: 884 1710 884
Size: 826 3772
Bme1580I G_kGCm'C
Cuts at: 903 2223 2316 3440 903
Size: 1320 93 1124 2061
Fragments arranged by size:
2061 1320 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1288 1810 1891 2226 4370 1288
Size: 522 81 335 2144 1516
Fragments arranged by size:
2144 1516 522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 873 2965 873
Size: 2092 2506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 1396
Size: 4598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1774 2653 3367 3665 1774
Size: 879 714 298 2707
Fragments arranged by size:
2707 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 209 4302 209
Size: 4093 505
BsaAI yAC'GTr
Cuts at: 1951 1951
Size: 4598
BsaBI GATnn'nnATC
Cuts at: 6 626 2112 4149 6
Size: 620 1486 2037 455
Fragments arranged by size:
2037 1486 620 455
BsaHI Gr'CG_yC
Cuts at: 1104 2029 2277 2979 4475 1104
Size: 925 248 702 1496 1227
Fragments arranged by size:
1496 1227 925 702 248
BsaWI w'CCGG_w
Cuts at: 363 1515 1787 2308 2986 3397 3544 3818 363
Size: 1152 272 521 678 411 147 274 1143
Fragments arranged by size:
1152 1143 678 521 411 274 272 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1000 1135 1207 1259 1261 1394 1000
Size: 135 72 52 2 133 4204
Fragments arranged by size:
4204 135 133 72 52 2
BseYI C'CCAG_C
Cuts at: 2023 2781 3446 3854 2023
Size: 758 665 408 2767
Fragments arranged by size:
2767 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1573 1573
Size: 4598
BsiEI CG_ry'CG
Cuts at: 104 2140 2186 3416 104
Size: 2036 46 1230 1286
Fragments arranged by size:
2036 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 903 1234 1612 2390 2580 3440 903
Size: 331 378 778 190 860 2061
Fragments arranged by size:
2061 860 778 378 331 190
BsmI GAATG_Cn'
Cuts at: 885 885
Size: 4598
Bsp1286I G_dGCh'C
Cuts at: 903 1234 1612 2223 2316 2390 2580 2642 3440
Size: 331 378 611 93 74 190 62 798
Cuts at: 3440 903
Size: 2061
Fragments arranged by size:
2061 798 611 378 331 190 93 74
62
BspEI T'CCGG_A
Cuts at: 363 363
Size: 4598
BspHI T'CATG_A
Cuts at: 3030 3030
Size: 4598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1648 1862 2164 2545 1648
Size: 214 302 381 3701
Fragments arranged by size:
3701 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2890 2944 4428 4593 50
Size: 2840 54 1484 165 55
Fragments arranged by size:
2840 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 245 2510 245
Size: 2265 2333
BsrFI r'CCGG_y
Cuts at: 1394 1515 2596 2777 1394
Size: 121 1081 181 3215
Fragments arranged by size:
3215 1081 181 121
BssSI C'ACGA_G
Cuts at: 2869 3577 2869
Size: 708 3890
BstAPI GCAn_nnn'nTGC
Cuts at: 1975 1975
Size: 4598
BstBI TT'CG_AA
Cuts at: 2959 2959
Size: 4598
BstYI r'GATC_y
Cuts at: 1 137 1623 2090 2113 2448 2694 3000 3012
Size: 136 1486 467 23 335 246 306 12
Cuts at: 3012 3098 4150 1
Size: 86 1052 449
Fragments arranged by size:
1486 1052 467 449 335 306 246 136
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 761 832 761
Size: 71 4527
BtgI C'CryG_G
Cuts at: 1526 1526
Size: 4598
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 709 2548 2729 2892 709
Size: 1839 181 163 2415
Fragments arranged by size:
2415 1839 181 163
BtsI GCAGTG_nn'
Cuts at: 1007 1369 1585 1841 1007
Size: 362 216 256 3764
Fragments arranged by size:
3764 362 256 216
DraI TTT'AAA
Cuts at: 1451 1597 1451
Size: 146 4452
DrdI GACnn_nn'nnGTC
Cuts at: 2304 3648 2304
Size: 1344 3254
EaeI y'GGCC_r
Cuts at: 1071 1441 2183 2357 2748 2775 4120 1071
Size: 370 742 174 391 27 1345 1549
Fragments arranged by size:
1549 1345 742 391 370 174 27
EagI C'GGCC_G
Cuts at: 2183 2183
Size: 4598
EarI CTCTTCn'nnn_
Cuts at: 696 1222 1238 1394 2621 2831 696
Size: 526 16 156 1227 210 2463
Fragments arranged by size:
2463 1227 526 210 156 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2806 3548 3694 4408 2806
Size: 742 146 714 2996
Fragments arranged by size:
2996 742 714 146
EcoICRI GAG'CTC
Cuts at: 1610 1610
Size: 4598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 2423 2855 3208 3870 1396
Size: 1027 432 353 662 2124
Fragments arranged by size:
2124 1027 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1737 1737
Size: 4598
EcoRI G'AATT_C
Cuts at: 934 1562 1602 934
Size: 628 40 3930
Fragments arranged by size:
3930 628 40
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1104 2617 1104
Size: 1513 3085
FspI TGC'GCA
Cuts at: 2379 2379
Size: 4598
HaeII r_GCGC'y
Cuts at: 2032 2280 3510 4281 2032
Size: 248 1230 771 2349
Fragments arranged by size:
2349 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1266 1298 1564 1596 2440 2470 2472 2502 1266
Size: 32 266 32 844 30 2 30 3362
Fragments arranged by size:
3362 844 266 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1540 1637 1540
Size: 97 4501
KasI G'GCGC_C
Cuts at: 2028 2276 2028
Size: 248 4350
KpnI G_GTAC'C
Cuts at: 1618 1618
Size: 4598
MfeI C'AATT_G
Cuts at: 251 464 251
Size: 213 4385
MluI A'CGCG_T
Cuts at: 4031 4031
Size: 4598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 96 955 1336 1390 3357 3541 96
Size: 859 381 54 1967 184 1153
Fragments arranged by size:
1967 1153 859 381 184 54
MscI TGG'CCA
Cuts at: 1073 2359 1073
Size: 1286 3312
MslI CAynn'nnrTG
Cuts at: 2587 2714 2587
Size: 127 4471
MspA1I CmG'CkG
Cuts at: 1709 2023 2383 3167 3412 1709
Size: 314 360 784 245 2895
Fragments arranged by size:
2895 784 360 314 245
NaeI GCC'GGC
Cuts at: 2779 2779
Size: 4598
NarI GG'CG_CC
Cuts at: 2029 2277 2029
Size: 248 4350
NgoMIV G'CCGG_C
Cuts at: 2777 2777
Size: 4598
NspI r_CATG'y
Cuts at: 300 771 1651 2151 3754 300
Size: 471 880 500 1603 1144
Fragments arranged by size:
1603 1144 880 500 471
PciI A'CATG_T
Cuts at: 3750 3750
Size: 4598
PflMI CCAn_nnn'nTGG
Cuts at: 607 607
Size: 4598
PmeI GTTT'AAAC
Cuts at: 1597 1597
Size: 4598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 4566
PstI C_TGCA'G
Cuts at: 1645 2330 1645
Size: 685 3913
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4598
PvuII CAG'CTG
Cuts at: 2023 2383 2023
Size: 360 4238
RsrII CG'GwC_CG
Cuts at: 2793 2793
Size: 4598
SacI G_AGCT'C
Cuts at: 1612 1612
Size: 4598
SalI G'TCGA_C
Cuts at: 1635 1635
Size: 4598
SapI GCTCTTCn'nnn_
Cuts at: 1222 1394 2621 2831 1222
Size: 172 1227 210 2989
Fragments arranged by size:
2989 1227 210 172
SbfI CC_TGCA'GG
Cuts at: 1645 1645
Size: 4598
ScaI AGT'ACT
Cuts at: 4115 4115
Size: 4598
SfcI C'TryA_G
Cuts at: 33 804 1098 1641 2326 3294 3485 33
Size: 771 294 543 685 968 191 1146
Fragments arranged by size:
1146 968 771 685 543 294 191
SfoI GGC'GCC
Cuts at: 2030 2278 2030
Size: 248 4350
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
SmaI CCC'GGG
Cuts at: 1620 1620
Size: 4598
SmlI C'TyrA_G
Cuts at: 1753 2668 3382 3644 1753
Size: 915 714 262 2707
Fragments arranged by size:
2707 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4598
SphI G_CATG'C
Cuts at: 1651 2151 1651
Size: 500 4098
SspI AAT'ATT
Cuts at: 484 484
Size: 4598
StyI C'CwwG_G
Cuts at: 1732 2086 1732
Size: 354 4244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2181 2314 2773 2982 2181
Size: 133 459 209 3797
Fragments arranged by size:
3797 459 209 133
TatI w'GTAC_w
Cuts at: 167 4113 167
Size: 3946 652
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 345 377 603 635 4115 4147 345
Size: 32 226 32 3480 32 796
Fragments arranged by size:
3480 796 226 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2395 2395
Size: 4598
XbaI T'CTAG_A
Cuts at: 1629 1629
Size: 4598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1165 2093 1165
Size: 928 3670
XmaI C'CCGG_G
Cuts at: 1618 1618
Size: 4598
XmnI GAAnn'nnTTC
Cuts at: 1084 1084
Size: 4598
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI