Restriction map for
pF1KA1346
(Circular) (Six-base) MAPSORT of: pF1KA1346.seq Check: 9661 from: 1 to: 6014
pF1KA1346 6014 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 3052 3052
Size: 6014
Acc65I G'GTAC_C
Cuts at: 3030 3030
Size: 6014
AclI AA'CG_TT
Cuts at: 5854 5854
Size: 6014
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3839 4271 5018 5286 3839
Size: 432 747 268 4567
Fragments arranged by size:
4567 747 432 268
AflIII A'CryG_T
Cuts at: 1204 1322 1621 3366 4470 5447 1204
Size: 118 299 1745 1104 977 1771
Fragments arranged by size:
1771 1745 1104 977 299 118
AgeI A'CCGG_T
Cuts at: 4402 4402
Size: 6014
AleI CACnn'nnGTG
Cuts at: 932 932
Size: 6014
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1930 1930
Size: 6014
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1369 1401 1369
Size: 32 5982
AlwNI CAG_nnn'CTG
Cuts at: 360 530 588 841 1259 1928 3319 4886 360
Size: 170 58 253 418 669 1391 1567 1488
Fragments arranged by size:
1567 1488 1391 669 418 253 170 58
ApaI G_GGCC'C
Cuts at: 203 203
Size: 6014
ApaLI G'TGCA_C
Cuts at: 4784 4784
Size: 6014
ApoI r'AATT_y
Cuts at: 922 2537 3018 5367 5623 922
Size: 1615 481 2349 256 1313
Fragments arranged by size:
2349 1615 1313 481 256
AseI AT'TA_AT
Cuts at: 20 2759 20
Size: 2739 3275
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6014
AvaI C'yCGr_G
Cuts at: 124 256 312 3034 124
Size: 132 56 2722 3104
Fragments arranged by size:
3104 2722 132 56
BamHI G'GATC_C
Cuts at: 2386 3039 2386
Size: 653 5361
BanI G'GyrC_C
Cuts at: 260 291 396 487 547 642 1543 1645 1699
Size: 31 105 91 60 95 901 102 54
Cuts at: 1699 3030 3444 3692 3727 260
Size: 1331 414 248 35 2547
Fragments arranged by size:
2547 1331 901 414 248 105 102 95
91 60 54 35 31
BanII G_rGCy'C
Cuts at: 180 203 722 2567 3028 4058 180
Size: 23 519 1845 461 1030 2136
Fragments arranged by size:
2136 1845 1030 519 461 23
BbeI G_GCGC'C
Cuts at: 264 400 551 3448 3696 264
Size: 136 151 2897 248 2582
Fragments arranged by size:
2897 2582 248 151 136
BbsI GAAGACnn'nnnn_
Cuts at: 755 2742 755
Size: 1987 4027
BbvCI CC'TCA_GC
Cuts at: 523 523
Size: 6014
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 893 927 90
Size: 34 769 34 5177
Fragments arranged by size:
5177 769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3912 4673 6014 3912
Size: 761 1341 3912
Fragments arranged by size:
3912 1341 761
BclI T'GATC_A
Cuts at: 3534 3534
Size: 6014
BglII A'GATC_T
Cuts at: 1031 3529 5566 1031
Size: 2498 2037 1479
Fragments arranged by size:
2498 2037 1479
BlpI GC'TnA_GC
Cuts at: 3126 3126
Size: 6014
Bme1580I G_kGCm'C
Cuts at: 125 203 3639 3732 4788 125
Size: 78 3436 93 1056 1351
Fragments arranged by size:
3436 1351 1056 93 78
BmrI ACTGGGnnnn_n'
Cuts at: 740 1127 1867 2832 3226 3307 3642 5786 740
Size: 387 740 965 394 81 335 2144 968
Fragments arranged by size:
2144 968 965 740 394 387 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4381 4381
Size: 6014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 388 397 2366 322
Size: 66 9 1969 3970
Fragments arranged by size:
3970 1969 66 9
Bpu10I CC'TnA_GC
Cuts at: 523 523
Size: 6014
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1957 2523 3190 4069 4561 4859 1957
Size: 566 667 879 492 298 3112
Fragments arranged by size:
3112 879 667 566 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 191 736 1442 5718 191
Size: 545 706 4276 487
Fragments arranged by size:
4276 706 545 487
BsaAI yAC'GTr
Cuts at: 698 1323 3367 698
Size: 625 2044 3345
Fragments arranged by size:
3345 2044 625
BsaBI GATnn'nnATC
Cuts at: 6 3528 5565 6
Size: 3522 2037 455
Fragments arranged by size:
3522 2037 455
BsaHI Gr'CG_yC
Cuts at: 261 397 445 548 1399 2060 3445 3693 4395
Size: 136 48 103 851 661 1385 248 702
Cuts at: 4395 5891 261
Size: 1496 384
Fragments arranged by size:
1496 1385 851 702 661 384 248 136
103 48
BsaWI w'CCGG_w
Cuts at: 453 3203 3724 4402 4676 4823 5234 453
Size: 2750 521 678 274 147 411 1233
Fragments arranged by size:
2750 1233 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2500 2530 2500
Size: 30 5984
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 286 295 1853 2508 286
Size: 9 1558 655 3792
Fragments arranged by size:
3792 1558 655 9
BseYI C'CCAG_C
Cuts at: 147 505 561 994 1790 2847 3439 4197 4774
Size: 358 56 433 796 1057 592 758 577
Cuts at: 4774 5270 147
Size: 496 891
Fragments arranged by size:
1057 891 796 758 592 577 496 433
358 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 2840 325
Size: 2515 3499
BsiEI CG_ry'CG
Cuts at: 104 727 3556 3602 4810 104
Size: 623 2829 46 1208 1308
Fragments arranged by size:
2829 1308 1208 623 46
BsiHKAI G_wGCw'C
Cuts at: 1154 3028 3806 3996 4788 1154
Size: 1874 778 190 792 2380
Fragments arranged by size:
2380 1874 792 778 190
BsiWI C'GTAC_G
Cuts at: 1550 1550
Size: 6014
BsmI GAATG_Cn'
Cuts at: 998 2752 3007 998
Size: 1754 255 4005
Fragments arranged by size:
4005 1754 255
BsmBI CGTCTCn'nnnn_
Cuts at: 436 2067 436
Size: 1631 4383
Bsp1286I G_dGCh'C
Cuts at: 125 180 203 722 1154 2567 3028 3639 3732
Size: 55 23 519 432 1413 461 611 93
Cuts at: 3732 3806 3996 4058 4788 125
Size: 74 190 62 730 1351
Fragments arranged by size:
1413 1351 730 611 519 461 432 190
93 74 62 55 23
BspEI T'CCGG_A
Cuts at: 453 5234 453
Size: 4781 1233
BspHI T'CATG_A
Cuts at: 2322 2940 5190 2322
Size: 618 2250 3146
Fragments arranged by size:
3146 2250 618
BspMI ACCTGCnnnn'nnnn_
Cuts at: 566 3064 3278 3580 3961 566
Size: 2498 214 302 381 2619
Fragments arranged by size:
2619 2498 381 302 214
BsrBI CCG'CTC
Cuts at: 50 174 4306 4360 5844 6009 50
Size: 124 4132 54 1484 165 55
Fragments arranged by size:
4132 1484 165 124 55 54
BsrDI GCAATG_nn'
Cuts at: 2601 3926 2601
Size: 1325 4689
BsrFI r'CCGG_y
Cuts at: 639 1562 1642 4012 4193 4402 639
Size: 923 80 2370 181 209 2251
Fragments arranged by size:
2370 2251 923 209 181 80
BsrGI T'GTAC_A
Cuts at: 983 2486 983
Size: 1503 4511
BssHII G'CGCG_C
Cuts at: 306 306
Size: 6014
BssSI C'ACGA_G
Cuts at: 4285 4643 4285
Size: 358 5656
BstAPI GCAn_nnn'nTGC
Cuts at: 1259 3391 1259
Size: 2132 3882
BstBI TT'CG_AA
Cuts at: 4375 4375
Size: 6014
BstXI CCAn_nnnn'nTGG
Cuts at: 900 934 900
Size: 34 5980
BstYI r'GATC_y
Cuts at: 1 363 1031 1924 2269 2386 3039 3506 3529
Size: 362 668 893 345 117 653 467 23
Cuts at: 3529 3864 4110 5122 5208 5220 5566 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 893 668 653 467 449 362 346
345 335 246 117 86 23 12
BtgI C'CryG_G
Cuts at: 227 927 1687 227
Size: 700 760 4554
Fragments arranged by size:
4554 760 700
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3964 4145 4308 3964
Size: 181 163 5670
Fragments arranged by size:
5670 181 163
BtsI GCAGTG_nn'
Cuts at: 471 811 939 1360 1452 1573 1599 3012 3257
Size: 340 128 421 92 121 26 1413 245
Cuts at: 3257 471
Size: 3228
Fragments arranged by size:
3228 1413 421 340 245 128 121 92
26
DraI TTT'AAA
Cuts at: 2960 3013 2960
Size: 53 5961
DraIII CAC_nnn'GTG
Cuts at: 1135 1634 1135
Size: 499 5515
DrdI GACnn_nn'nnGTC
Cuts at: 1873 3720 4578 1873
Size: 1847 858 3309
Fragments arranged by size:
3309 1847 858
EaeI y'GGCC_r
Cuts at: 239 724 3599 3773 4164 4191 5536 239
Size: 485 2875 174 391 27 1345 717
Fragments arranged by size:
2875 1345 717 485 391 174 27
EagI C'GGCC_G
Cuts at: 724 3599 724
Size: 2875 3139
EarI CTCTTCn'nnn_
Cuts at: 2684 4037 4247 2684
Size: 1353 210 4451
Fragments arranged by size:
4451 1353 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 325 684 1708 4222 4532 4678 5824 325
Size: 359 1024 2514 310 146 1146 515
Fragments arranged by size:
2514 1146 1024 515 359 310 146
EcoICRI GAG'CTC
Cuts at: 3026 3026
Size: 6014
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 388 397 2366 3839 4271 5018 5286 322
Size: 66 9 1969 1473 432 747 268 1050
Fragments arranged by size:
1969 1473 1050 747 432 268 66 9
EcoNI CCTnn'n_nnAGG
Cuts at: 532 532
Size: 6014
EcoO109I rG'GnC_Cy
Cuts at: 784 804 1196 1804 3153 784
Size: 20 392 608 1349 3645
Fragments arranged by size:
3645 1349 608 392 20
EcoRI G'AATT_C
Cuts at: 922 2537 3018 922
Size: 1615 481 3918
Fragments arranged by size:
3918 1615 481
EcoRV GAT'ATC
Cuts at: 2320 2328 2320
Size: 8 6006
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4033 4033
Size: 6014
FspI TGC'GCA
Cuts at: 3795 3795
Size: 6014
HaeII r_GCGC'y
Cuts at: 235 264 400 551 609 3448 3696 4718 5697
Size: 29 136 151 58 2839 248 1022 979
Cuts at: 5697 235
Size: 552
Fragments arranged by size:
2839 1022 979 552 248 151 136 58
29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 493 525 2155 2187 2845 2877 2937 2969 3856
Size: 32 1630 32 658 32 60 32 887
Cuts at: 3856 3886 3888 3918 493
Size: 30 2 30 2589
Fragments arranged by size:
2589 1630 887 658 60 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3053 3053
Size: 6014
HindIII A'AGCT_T
Cuts at: 2009 2901 2009
Size: 892 5122
KasI G'GCGC_C
Cuts at: 260 396 547 3444 3692 260
Size: 136 151 2897 248 2582
Fragments arranged by size:
2897 2582 248 151 136
KpnI G_GTAC'C
Cuts at: 3034 3034
Size: 6014
MluI A'CGCG_T
Cuts at: 5447 5447
Size: 6014
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 227 1212 1436 2365 4685 4869 227
Size: 985 224 929 2320 184 1372
Fragments arranged by size:
2320 1372 985 929 224 184
MscI TGG'CCA
Cuts at: 3775 3775
Size: 6014
MslI CAynn'nnrTG
Cuts at: 881 898 932 1343 2362 4003 4130 881
Size: 17 34 411 1019 1641 127 2765
Fragments arranged by size:
2765 1641 1019 411 127 34 17
MspA1I CmG'CkG
Cuts at: 229 360 588 1706 2373 2513 2847 3125 3439
Size: 131 228 1118 667 140 334 278 314
Cuts at: 3439 3799 4812 5057 229
Size: 360 1013 245 1186
Fragments arranged by size:
1186 1118 1013 667 360 334 314 278
245 228 140 131
NaeI GCC'GGC
Cuts at: 641 4195 641
Size: 3554 2460
NarI GG'CG_CC
Cuts at: 261 397 548 3445 3693 261
Size: 136 151 2897 248 2582
Fragments arranged by size:
2897 2582 248 151 136
NgoMIV G'CCGG_C
Cuts at: 639 4193 639
Size: 3554 2460
NspI r_CATG'y
Cuts at: 1208 1335 1625 3067 3567 4474 1208
Size: 127 290 1442 500 907 2748
Fragments arranged by size:
2748 1442 907 500 290 127
PasI CC'CwG_GG
Cuts at: 625 625
Size: 6014
PciI A'CATG_T
Cuts at: 1204 1621 4470 1204
Size: 417 2849 2748
Fragments arranged by size:
2849 2748 417
PflMI CCAn_nnn'nTGG
Cuts at: 360 420 915 1395 1885 360
Size: 60 495 480 490 4489
Fragments arranged by size:
4489 495 490 480 60
PfoI T'CCnGG_A
Cuts at: 2389 2389
Size: 6014
PmeI GTTT'AAAC
Cuts at: 3013 3013
Size: 6014
PmlI CAC'GTG
Cuts at: 698 1323 698
Size: 625 5389
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5153 5185 5153
Size: 32 5982
PpuMI rG'GwC_Cy
Cuts at: 804 1196 804
Size: 392 5622
PspOMI G'GGCC_C
Cuts at: 199 199
Size: 6014
PstI C_TGCA'G
Cuts at: 3061 3746 3061
Size: 685 5329
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6014
PvuII CAG'CTG
Cuts at: 360 1706 2847 3439 3799 360
Size: 1346 1141 592 360 2575
Fragments arranged by size:
2575 1346 1141 592 360
RsrII CG'GwC_CG
Cuts at: 4209 4209
Size: 6014
SacI G_AGCT'C
Cuts at: 3028 3028
Size: 6014
SacII CC_GC'GG
Cuts at: 230 230
Size: 6014
SalI G'TCGA_C
Cuts at: 3051 3051
Size: 6014
SanDI GG'GwC_CC
Cuts at: 1196 1196
Size: 6014
SapI GCTCTTCn'nnn_
Cuts at: 4037 4247 4037
Size: 210 5804
SbfI CC_TGCA'GG
Cuts at: 3061 3061
Size: 6014
ScaI AGT'ACT
Cuts at: 1897 5531 1897
Size: 3634 2380
SfcI C'TryA_G
Cuts at: 33 649 1920 3057 3742 4735 4926 33
Size: 616 1271 1137 685 993 191 1121
Fragments arranged by size:
1271 1137 1121 993 685 616 191
SfoI GGC'GCC
Cuts at: 262 398 549 3446 3694 262
Size: 136 151 2897 248 2582
Fragments arranged by size:
2897 2582 248 151 136
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6014
SmaI CCC'GGG
Cuts at: 314 3036 314
Size: 2722 3292
SmlI C'TyrA_G
Cuts at: 1936 2502 3169 4084 4576 4838 1936
Size: 566 667 915 492 262 3112
Fragments arranged by size:
3112 915 667 566 492 262
SpeI A'CTAG_T
Cuts at: 67 2302 67
Size: 2235 3779
SphI G_CATG'C
Cuts at: 3067 3567 3067
Size: 500 5514
StyI C'CwwG_G
Cuts at: 1432 1807 2132 3148 3502 1432
Size: 375 325 1016 354 3944
Fragments arranged by size:
3944 1016 375 354 325
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 760 1146 3597 3730 4189 4398 760
Size: 386 2451 133 459 209 2376
Fragments arranged by size:
2451 2376 459 386 209 133
TatI w'GTAC_w
Cuts at: 983 1847 1895 2486 5529 983
Size: 864 48 591 3043 1468
Fragments arranged by size:
3043 1468 864 591 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 638 670 1884 1916 5531 5563 638
Size: 32 1214 32 3615 32 1089
Fragments arranged by size:
3615 1214 1089 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3811 3811
Size: 6014
XbaI T'CTAG_A
Cuts at: 3045 3045
Size: 6014
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1579 3509 1579
Size: 1930 4084
XmaI C'CCGG_G
Cuts at: 312 3034 312
Size: 2722 3292
XmnI GAAnn'nnTTC
Cuts at: 2042 2537 2042
Size: 495 5519
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AscI AvrII BaeI
BfrBI BglI BmgBI BmtI BstEII BstZ17I Bsu36I ClaI
CspCI FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspXI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI