Restriction map for
pF1KA1351
(Circular) (Six-base) MAPSORT of: pF1KA1351.seq Check: 4600 from: 1 to: 6785
pF1KA1351 6785 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1023 3823 1023
Size: 2800 3985
Acc65I G'GTAC_C
Cuts at: 3801 3801
Size: 6785
AclI AA'CG_TT
Cuts at: 6625 6625
Size: 6785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1167 2450 4610 5042 5789 6057 835
Size: 332 1283 2160 432 747 268 1563
Fragments arranged by size:
2160 1563 1283 747 432 332 268
AflIII A'CryG_T
Cuts at: 884 1050 4137 5241 6218 884
Size: 166 3087 1104 977 1451
Fragments arranged by size:
3087 1451 1104 977 166
AgeI A'CCGG_T
Cuts at: 5173 5173
Size: 6785
AleI CACnn'nnGTG
Cuts at: 1652 1652
Size: 6785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3360 3360
Size: 6785
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1284 1316 1284
Size: 32 6753
AlwNI CAG_nnn'CTG
Cuts at: 697 1566 3120 3156 4090 5657 697
Size: 869 1554 36 934 1567 1825
Fragments arranged by size:
1825 1567 1554 934 869 36
ApaI G_GGCC'C
Cuts at: 162 162
Size: 6785
ApaLI G'TGCA_C
Cuts at: 1456 5555 1456
Size: 4099 2686
ApoI r'AATT_y
Cuts at: 794 826 1284 1343 2298 2304 2781 2824 3040
Size: 32 458 59 955 6 477 43 216
Cuts at: 3040 3789 6138 6394 794
Size: 749 2349 256 1185
Fragments arranged by size:
2349 1185 955 749 477 458 256 216
59 43 32 6
AseI AT'TA_AT
Cuts at: 20 2404 20
Size: 2384 4401
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6785
AvaI C'yCGr_G
Cuts at: 902 2009 2132 2972 3805 902
Size: 1107 123 840 833 3882
Fragments arranged by size:
3882 1107 840 833 123
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2261 2294 2261
Size: 33 6752
BamHI G'GATC_C
Cuts at: 3810 3810
Size: 6785
BanI G'GyrC_C
Cuts at: 553 766 2633 2843 3801 4215 4463 4498 553
Size: 213 1867 210 958 414 248 35 2840
Fragments arranged by size:
2840 1867 958 414 248 213 210 35
BanII G_rGCy'C
Cuts at: 162 3799 4829 162
Size: 3637 1030 2118
Fragments arranged by size:
3637 2118 1030
BbeI G_GCGC'C
Cuts at: 4219 4467 4219
Size: 248 6537
BciVI GTATCCnnnnn_n'
Cuts at: 2340 2433 4683 5444 6785 2340
Size: 93 2250 761 1341 2340
Fragments arranged by size:
2340 2250 1341 761 93
BclI T'GATC_A
Cuts at: 2075 3090 4305 2075
Size: 1015 1215 4555
Fragments arranged by size:
4555 1215 1015
BglII A'GATC_T
Cuts at: 1060 4300 6337 1060
Size: 3240 2037 1508
Fragments arranged by size:
3240 2037 1508
BlpI GC'TnA_GC
Cuts at: 3897 3897
Size: 6785
Bme1580I G_kGCm'C
Cuts at: 162 1460 2638 4410 4503 5559 162
Size: 1298 1178 1772 93 1056 1388
Fragments arranged by size:
1772 1388 1298 1178 1056 93
BmgBI CAC'GTC
Cuts at: 3012 3012
Size: 6785
BmrI ACTGGGnnnn_n'
Cuts at: 201 3997 4078 4413 6557 201
Size: 3796 81 335 2144 429
Fragments arranged by size:
3796 2144 429 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3715 5152 3715
Size: 1437 5348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1903 1903
Size: 6785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 946 1246 1447 3574 3769 3961 4840 5332
Size: 155 300 201 2127 195 192 879 492
Cuts at: 5332 5630 791
Size: 298 1946
Fragments arranged by size:
2127 1946 879 492 300 298 201 195
192 155
BsaI GGTCTCn'nnnn_
Cuts at: 6489 6489
Size: 6785
BsaAI yAC'GTr
Cuts at: 1051 4138 1051
Size: 3087 3698
BsaBI GATnn'nnATC
Cuts at: 6 913 1106 4299 6336 6
Size: 907 193 3193 2037 455
Fragments arranged by size:
3193 2037 907 455 193
BsaHI Gr'CG_yC
Cuts at: 3341 4216 4464 5166 6662 3341
Size: 875 248 702 1496 3464
Fragments arranged by size:
3464 1496 875 702 248
BsaWI w'CCGG_w
Cuts at: 3974 4495 5173 5447 5594 6005 3974
Size: 521 678 274 147 411 4754
Fragments arranged by size:
4754 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2417 2447 2937 2967 2417
Size: 30 490 30 6235
Fragments arranged by size:
6235 490 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2755 2960 3430 3490 2755
Size: 205 470 60 6050
Fragments arranged by size:
6050 470 205 60
BseYI C'CCAG_C
Cuts at: 735 1417 4210 4968 5545 6041 735
Size: 682 2793 758 577 496 1479
Fragments arranged by size:
2793 1479 758 682 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2869 2899 3169 3191 2869
Size: 30 270 22 6463
Fragments arranged by size:
6463 270 30 22
BsiEI CG_ry'CG
Cuts at: 104 4327 4373 5581 104
Size: 4223 46 1208 1308
Fragments arranged by size:
4223 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 536 1460 2214 3017 3253 3799 4577 4767 5559
Size: 924 754 803 236 546 778 190 792
Cuts at: 5559 536
Size: 1762
Fragments arranged by size:
1762 924 803 792 778 754 546 236
190
BsmI GAATG_Cn'
Cuts at: 546 1218 546
Size: 672 6113
BsmBI CGTCTCn'nnnn_
Cuts at: 3526 3526
Size: 6785
Bsp1286I G_dGCh'C
Cuts at: 162 536 1460 2214 2638 3017 3253 3799 4410
Size: 374 924 754 424 379 236 546 611
Cuts at: 4410 4503 4577 4767 4829 5559 162
Size: 93 74 190 62 730 1388
Fragments arranged by size:
1388 924 754 730 611 546 424 379
374 236 190 93 74 62
BspEI T'CCGG_A
Cuts at: 6005 6005
Size: 6785
BspHI T'CATG_A
Cuts at: 1247 5961 1247
Size: 4714 2071
BspMI ACCTGCnnnn'nnnn_
Cuts at: 872 2257 2921 3362 3835 4049 4351 4732 872
Size: 1385 664 441 473 214 302 381 2925
Fragments arranged by size:
2925 1385 664 473 441 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5077 5131 6615 6780 50
Size: 5027 54 1484 165 55
Fragments arranged by size:
5027 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1778 1809 2417 4697 1778
Size: 31 608 2280 3866
Fragments arranged by size:
3866 2280 608 31
BsrFI r'CCGG_y
Cuts at: 4783 4964 5173 4783
Size: 181 209 6395
Fragments arranged by size:
6395 209 181
BsrGI T'GTAC_A
Cuts at: 2414 3106 2414
Size: 692 6093
BssHII G'CGCG_C
Cuts at: 142 142
Size: 6785
BssSI C'ACGA_G
Cuts at: 5056 5414 5056
Size: 358 6427
BstAPI GCAn_nnn'nTGC
Cuts at: 3655 4162 3655
Size: 507 6278
BstBI TT'CG_AA
Cuts at: 1056 5146 1056
Size: 4090 2695
BstEII G'GTnAC_C
Cuts at: 1961 1961
Size: 6785
BstXI CCAn_nnnn'nTGG
Cuts at: 340 3261 340
Size: 2921 3864
BstYI r'GATC_y
Cuts at: 1 431 1060 1101 1107 3810 4277 4300 4635
Size: 430 629 41 6 2703 467 23 335
Cuts at: 4635 4881 5893 5979 5991 6337 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2703 1012 629 467 449 430 346 335
246 86 41 23 12 6
Bsu36I CC'TnA_GG
Cuts at: 690 3413 690
Size: 2723 4062
BtgI C'CryG_G
Cuts at: 2018 2018
Size: 6785
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2238 4735 4916 5079 2238
Size: 2497 181 163 3944
Fragments arranged by size:
3944 2497 181 163
BtsI GCAGTG_nn'
Cuts at: 1564 3635 4028 1564
Size: 2071 393 4321
Fragments arranged by size:
4321 2071 393
DraI TTT'AAA
Cuts at: 2303 3784 2303
Size: 1481 5304
DrdI GACnn_nn'nnGTC
Cuts at: 4491 5349 4491
Size: 858 5927
EaeI y'GGCC_r
Cuts at: 752 2163 4370 4544 4935 4962 6307 752
Size: 1411 2207 174 391 27 1345 1230
Fragments arranged by size:
2207 1411 1345 1230 391 174 27
EagI C'GGCC_G
Cuts at: 4370 4370
Size: 6785
EarI CTCTTCn'nnn_
Cuts at: 272 565 1975 3770 4808 5018 272
Size: 293 1410 1795 1038 210 2039
Fragments arranged by size:
2039 1795 1410 1038 293 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4993 5303 5449 6595 4993
Size: 310 146 1146 5183
Fragments arranged by size:
5183 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3797 3797
Size: 6785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1167 1903 2450 4610 5042 5789 6057 835
Size: 332 736 547 2160 432 747 268 1563
Fragments arranged by size:
2160 1563 747 736 547 432 332 268
EcoNI CCTnn'n_nnAGG
Cuts at: 689 2387 689
Size: 1698 5087
EcoO109I rG'GnC_Cy
Cuts at: 158 159 694 2657 3238 3924 158
Size: 1 535 1963 581 686 3019
Fragments arranged by size:
3019 1963 686 581 535 1
EcoRI G'AATT_C
Cuts at: 826 1343 2781 2824 3789 826
Size: 517 1438 43 965 3822
Fragments arranged by size:
3822 1438 965 517 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4804 4804
Size: 6785
FspI TGC'GCA
Cuts at: 4566 4566
Size: 6785
HaeII r_GCGC'y
Cuts at: 4219 4467 5489 6468 4219
Size: 248 1022 979 4536
Fragments arranged by size:
4536 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3736 3768 4627 4657 4659 4689 3736
Size: 32 859 30 2 30 5832
Fragments arranged by size:
5832 859 32 30 30 2
HincII GTy'rAC
Cuts at: 1535 2618 2733 3083 3447 3824 1535
Size: 1083 115 350 364 377 4496
Fragments arranged by size:
4496 1083 377 364 350 115
HindIII A'AGCT_T
Cuts at: 3581 3581
Size: 6785
HpaI GTT'AAC
Cuts at: 2618 2618
Size: 6785
KasI G'GCGC_C
Cuts at: 4215 4463 4215
Size: 248 6537
KpnI G_GTAC'C
Cuts at: 3805 3805
Size: 6785
MfeI C'AATT_G
Cuts at: 1412 1412
Size: 6785
MluI A'CGCG_T
Cuts at: 6218 6218
Size: 6785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1789 3140 5456 5640 1789
Size: 1351 2316 184 2934
Fragments arranged by size:
2934 2316 1351 184
MscI TGG'CCA
Cuts at: 754 2165 4546 754
Size: 1411 2381 2993
Fragments arranged by size:
2993 2381 1411
MslI CAynn'nnrTG
Cuts at: 1031 1552 1652 1822 4774 4901 1031
Size: 521 100 170 2952 127 2915
Fragments arranged by size:
2952 2915 521 170 127 100
MspA1I CmG'CkG
Cuts at: 3724 3896 4210 4570 5583 5828 3724
Size: 172 314 360 1013 245 4681
Fragments arranged by size:
4681 1013 360 314 245 172
NaeI GCC'GGC
Cuts at: 4966 4966
Size: 6785
NarI GG'CG_CC
Cuts at: 4216 4464 4216
Size: 248 6537
NcoI C'CATG_G
Cuts at: 2018 2018
Size: 6785
NgoMIV G'CCGG_C
Cuts at: 4964 4964
Size: 6785
NspI r_CATG'y
Cuts at: 445 888 3838 4338 5245 445
Size: 443 2950 500 907 1985
Fragments arranged by size:
2950 1985 907 500 443
PasI CC'CwG_GG
Cuts at: 697 697
Size: 6785
PciI A'CATG_T
Cuts at: 884 5241 884
Size: 4357 2428
PflMI CCAn_nnn'nTGG
Cuts at: 563 2227 563
Size: 1664 5121
PfoI T'CCnGG_A
Cuts at: 455 455
Size: 6785
PmeI GTTT'AAAC
Cuts at: 3784 3784
Size: 6785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5924 5956 5924
Size: 32 6753
PshAI GACnn'nnGTC
Cuts at: 2035 2035
Size: 6785
PsiI TTA'TAA
Cuts at: 1067 1067
Size: 6785
PspOMI G'GGCC_C
Cuts at: 158 158
Size: 6785
PstI C_TGCA'G
Cuts at: 1272 2090 3339 3359 3832 4517 1272
Size: 818 1249 20 473 685 3540
Fragments arranged by size:
3540 1249 818 685 473 20
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6785
PvuII CAG'CTG
Cuts at: 3724 4210 4570 3724
Size: 486 360 5939
Fragments arranged by size:
5939 486 360
RsrII CG'GwC_CG
Cuts at: 4980 4980
Size: 6785
SacI G_AGCT'C
Cuts at: 3799 3799
Size: 6785
SalI G'TCGA_C
Cuts at: 3822 3822
Size: 6785
SapI GCTCTTCn'nnn_
Cuts at: 565 1975 4808 5018 565
Size: 1410 2833 210 2332
Fragments arranged by size:
2833 2332 1410 210
SbfI CC_TGCA'GG
Cuts at: 3832 3832
Size: 6785
ScaI AGT'ACT
Cuts at: 6302 6302
Size: 6785
SfcI C'TryA_G
Cuts at: 33 1268 1349 1360 2086 3064 3335 3355 3828
Size: 1235 81 11 726 978 271 20 473
Cuts at: 3828 4513 5506 5697 33
Size: 685 993 191 1121
Fragments arranged by size:
1235 1121 993 978 726 685 473 271
191 81 20 11
SfoI GGC'GCC
Cuts at: 4217 4465 4217
Size: 248 6537
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6785
SmaI CCC'GGG
Cuts at: 2134 3807 2134
Size: 1673 5112
SmlI C'TyrA_G
Cuts at: 806 902 925 1261 1462 2009 2972 3589 3748
Size: 96 23 336 201 547 963 617 159
Cuts at: 3748 3940 4855 5347 5609 806
Size: 192 915 492 262 1982
Fragments arranged by size:
1982 963 915 617 547 492 336 262
201 192 159 96 23
SpeI A'CTAG_T
Cuts at: 67 1687 67
Size: 1620 5165
SphI G_CATG'C
Cuts at: 445 3838 4338 445
Size: 3393 500 2892
Fragments arranged by size:
3393 2892 500
StyI C'CwwG_G
Cuts at: 2018 2653 2695 3758 3919 4273 2018
Size: 635 42 1063 161 354 4530
Fragments arranged by size:
4530 1063 635 354 161 42
SwaI ATTT'AAAT
Cuts at: 2303 2303
Size: 6785
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 934 2349 2543 4368 4501 4960 5169 934
Size: 1415 194 1825 133 459 209 2550
Fragments arranged by size:
2550 1825 1415 459 209 194 133
TatI w'GTAC_w
Cuts at: 2414 3106 6300 2414
Size: 692 3194 2899
Fragments arranged by size:
3194 2899 692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 451 483 957 989 2417 2449 2908 2937 2940
Size: 32 474 32 1428 32 459 29 3
Cuts at: 2940 2969 6302 6334 451
Size: 29 3333 32 902
Fragments arranged by size:
3333 1428 902 474 459 32 32 32
32 29 29 3
Tth111I GACn'n_nGTC
Cuts at: 4582 4582
Size: 6785
XbaI T'CTAG_A
Cuts at: 3816 3816
Size: 6785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1615 2698 3261 3291 4280 1615
Size: 1083 563 30 989 4120
Fragments arranged by size:
4120 1083 989 563 30
XhoI C'TCGA_G
Cuts at: 902 2009 2972 902
Size: 1107 963 4715
Fragments arranged by size:
4715 1107 963
XmaI C'CCGG_G
Cuts at: 2132 3805 2132
Size: 1673 5112
XmnI GAAnn'nnTTC
Cuts at: 2677 2677
Size: 6785
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AscI AvrII BbsI
BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI NdeI NheI
NotI NruI NsiI PacI PmlI PpuMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI ZraI