Restriction map for
pF1KA1352
(Circular) (Six-base) MAPSORT of: pF1KA1352.seq Check: 7070 from: 1 to: 6641
pF1KA1352 6641 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1862 1862
Size: 6641
AccI GT'mk_AC
Cuts at: 704 817 3679 704
Size: 113 2862 3666
Fragments arranged by size:
3666 2862 113
Acc65I G'GTAC_C
Cuts at: 332 3657 332
Size: 3325 3316
AclI AA'CG_TT
Cuts at: 6481 6481
Size: 6641
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 3073 4466 4898 5645 5913 653
Size: 2420 1393 432 747 268 1381
Fragments arranged by size:
2420 1393 1381 747 432 268
AflIII A'CryG_T
Cuts at: 296 3993 5097 6074 296
Size: 3697 1104 977 863
Fragments arranged by size:
3697 1104 977 863
AgeI A'CCGG_T
Cuts at: 5029 5029
Size: 6641
AleI CACnn'nnGTG
Cuts at: 1267 2703 1267
Size: 1436 5205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1870 1870
Size: 6641
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1550 1582 2636 2668 1550
Size: 32 1054 32 5523
Fragments arranged by size:
5523 1054 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1875 2776 3946 5513 1875
Size: 901 1170 1567 3003
Fragments arranged by size:
3003 1567 1170 901
ApaLI G'TGCA_C
Cuts at: 5411 5411
Size: 6641
ApoI r'AATT_y
Cuts at: 625 802 1175 1457 1785 2095 2305 3145 3572
Size: 177 373 282 328 310 210 840 427
Cuts at: 3572 3645 5994 6250 625
Size: 73 2349 256 1016
Fragments arranged by size:
2349 1016 840 427 373 328 310 282
256 210 177 73
AseI AT'TA_AT
Cuts at: 20 1162 20
Size: 1142 5499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6641
AvaI C'yCGr_G
Cuts at: 3661 3661
Size: 6641
BamHI G'GATC_C
Cuts at: 3666 3666
Size: 6641
BanI G'GyrC_C
Cuts at: 332 1143 1392 3657 4071 4319 4354 332
Size: 811 249 2265 414 248 35 2619
Fragments arranged by size:
2619 2265 811 414 249 248 35
BanII G_rGCy'C
Cuts at: 3655 4685 3655
Size: 1030 5611
BbeI G_GCGC'C
Cuts at: 4075 4323 4075
Size: 248 6393
BbsI GAAGACnn'nnnn_
Cuts at: 1588 2884 3263 3526 1588
Size: 1296 379 263 4703
Fragments arranged by size:
4703 1296 379 263
BciVI GTATCCnnnnn_n'
Cuts at: 174 2114 4539 5300 6641 174
Size: 1940 2425 761 1341 174
Fragments arranged by size:
2425 1940 1341 761 174
BclI T'GATC_A
Cuts at: 4161 4161
Size: 6641
BfrBI ATG'CAT
Cuts at: 208 208
Size: 6641
BglII A'GATC_T
Cuts at: 4156 6193 4156
Size: 2037 4604
BlpI GC'TnA_GC
Cuts at: 3753 3753
Size: 6641
Bme1580I G_kGCm'C
Cuts at: 4266 4359 5415 4266
Size: 93 1056 5492
Fragments arranged by size:
5492 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2457 3853 3934 4269 6413 2457
Size: 1396 81 335 2144 2685
Fragments arranged by size:
2685 2144 1396 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5008 5008
Size: 6641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 893 1631 3127 893
Size: 738 1496 4407
Fragments arranged by size:
4407 1496 738
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1812 2830 3220 3817 4696 5188 5486 943
Size: 869 1018 390 597 879 492 298 2098
Fragments arranged by size:
2098 1018 879 869 597 492 390 298
BsaI GGTCTCn'nnnn_
Cuts at: 991 1768 6345 991
Size: 777 4577 1287
Fragments arranged by size:
4577 1287 777
BsaAI yAC'GTr
Cuts at: 2974 3994 2974
Size: 1020 5621
BsaBI GATnn'nnATC
Cuts at: 6 737 4155 6192 6
Size: 731 3418 2037 455
Fragments arranged by size:
3418 2037 731 455
BsaHI Gr'CG_yC
Cuts at: 279 711 4072 4320 5022 6518 279
Size: 432 3361 248 702 1496 402
Fragments arranged by size:
3361 1496 702 432 402 248
BsaWI w'CCGG_w
Cuts at: 2654 3830 4351 5029 5303 5450 5861 2654
Size: 1176 521 678 274 147 411 3434
Fragments arranged by size:
3434 1176 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 866 896 2222 2252 866
Size: 30 1326 30 5255
Fragments arranged by size:
5255 1326 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1786 2227 2671 2790 1786
Size: 441 444 119 5637
Fragments arranged by size:
5637 444 441 119
BseYI C'CCAG_C
Cuts at: 1974 3329 4066 4824 5401 5897 1974
Size: 1355 737 758 577 496 2718
Fragments arranged by size:
2718 1355 758 737 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 1181 361
Size: 820 5821
BsiEI CG_ry'CG
Cuts at: 104 4183 4229 5437 104
Size: 4079 46 1208 1308
Fragments arranged by size:
4079 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2714 3655 4433 4623 5415 2714
Size: 941 778 190 792 3940
Fragments arranged by size:
3940 941 792 778 190
BsmI GAATG_Cn'
Cuts at: 395 2327 2517 2757 395
Size: 1932 190 240 4279
Fragments arranged by size:
4279 1932 240 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1051 2127 1051
Size: 1076 5565
Bsp1286I G_dGCh'C
Cuts at: 2714 3655 4266 4359 4433 4623 4685 5415 2714
Size: 941 611 93 74 190 62 730 3940
Fragments arranged by size:
3940 941 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5861 5861
Size: 6641
BspHI T'CATG_A
Cuts at: 858 3560 5817 858
Size: 2702 2257 1682
Fragments arranged by size:
2702 2257 1682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1862 2383 2761 3691 3905 4207 4588 1862
Size: 521 378 930 214 302 381 3915
Fragments arranged by size:
3915 930 521 381 378 302 214
BsrBI CCG'CTC
Cuts at: 50 4933 4987 6471 6636 50
Size: 4883 54 1484 165 55
Fragments arranged by size:
4883 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2910 3020 4553 2910
Size: 110 1533 4998
Fragments arranged by size:
4998 1533 110
BsrFI r'CCGG_y
Cuts at: 1389 4639 4820 5029 1389
Size: 3250 181 209 3001
Fragments arranged by size:
3250 3001 209 181
BsrGI T'GTAC_A
Cuts at: 2408 2408
Size: 6641
BssSI C'ACGA_G
Cuts at: 4912 5270 4912
Size: 358 6283
BstAPI GCAn_nnn'nTGC
Cuts at: 4018 4018
Size: 6641
BstBI TT'CG_AA
Cuts at: 5002 5002
Size: 6641
BstXI CCAn_nnnn'nTGG
Cuts at: 2373 2925 2373
Size: 552 6089
BstYI r'GATC_y
Cuts at: 1 562 2133 3666 4133 4156 4491 4737 5749
Size: 561 1571 1533 467 23 335 246 1012
Cuts at: 5749 5835 5847 6193 1
Size: 86 12 346 449
Fragments arranged by size:
1571 1533 1012 561 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 818 818
Size: 6641
Bsu36I CC'TnA_GG
Cuts at: 890 1800 890
Size: 910 5731
BtgI C'CryG_G
Cuts at: 107 2381 107
Size: 2274 4367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4591 4772 4935 4591
Size: 181 163 6297
Fragments arranged by size:
6297 181 163
BtsI GCAGTG_nn'
Cuts at: 1876 3884 1876
Size: 2008 4633
DraI TTT'AAA
Cuts at: 683 1554 2559 3640 683
Size: 871 1005 1081 3684
Fragments arranged by size:
3684 1081 1005 871
DrdI GACnn_nn'nnGTC
Cuts at: 3016 4347 5205 3016
Size: 1331 858 4452
Fragments arranged by size:
4452 1331 858
EaeI y'GGCC_r
Cuts at: 2185 3342 4226 4400 4791 4818 6163 2185
Size: 1157 884 174 391 27 1345 2663
Fragments arranged by size:
2663 1345 1157 884 391 174 27
EagI C'GGCC_G
Cuts at: 4226 4226
Size: 6641
EarI CTCTTCn'nnn_
Cuts at: 464 470 608 1709 4664 4874 464
Size: 6 138 1101 2955 210 2231
Fragments arranged by size:
2955 2231 1101 210 138 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 4849 5159 5305 6451 127
Size: 4722 310 146 1146 317
Fragments arranged by size:
4722 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 3653 3653
Size: 6641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 893 1631 3073 3127 4466 4898 5645 5913
Size: 240 738 1442 54 1339 432 747 268
Cuts at: 5913 653
Size: 1381
Fragments arranged by size:
1442 1381 1339 747 738 432 268 240
54
EcoNI CCTnn'n_nnAGG
Cuts at: 1936 2768 3502 1936
Size: 832 734 5075
Fragments arranged by size:
5075 832 734
EcoO109I rG'GnC_Cy
Cuts at: 2773 3118 3478 3780 2773
Size: 345 360 302 5634
Fragments arranged by size:
5634 360 345 302
EcoRI G'AATT_C
Cuts at: 2095 3645 2095
Size: 1550 5091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1301 1356 4660 1301
Size: 55 3304 3282
Fragments arranged by size:
3304 3282 55
FspI TGC'GCA
Cuts at: 4422 4422
Size: 6641
HaeII r_GCGC'y
Cuts at: 4075 4323 5345 6324 4075
Size: 248 1022 979 4392
Fragments arranged by size:
4392 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2222 2242 2254 2274 2828 2860 3105 3137 3479
Size: 20 12 20 554 32 245 32 342
Cuts at: 3479 3511 4483 4513 4515 4545 2222
Size: 32 972 30 2 30 4318
Fragments arranged by size:
4318 972 554 342 245 32 32 32
30 30 20 20 12 2
HincII GTy'rAC
Cuts at: 3680 3680
Size: 6641
HindIII A'AGCT_T
Cuts at: 2564 2564
Size: 6641
KasI G'GCGC_C
Cuts at: 4071 4319 4071
Size: 248 6393
KpnI G_GTAC'C
Cuts at: 336 3661 336
Size: 3325 3316
MluI A'CGCG_T
Cuts at: 6074 6074
Size: 6641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 192 864 1313 5312 5496 192
Size: 672 449 3999 184 1337
Fragments arranged by size:
3999 1337 672 449 184
MscI TGG'CCA
Cuts at: 3344 4402 3344
Size: 1058 5583
MslI CAynn'nnrTG
Cuts at: 1267 1540 1740 2703 2705 2923 4630 4757 1267
Size: 273 200 963 2 218 1707 127 3151
Fragments arranged by size:
3151 1707 963 273 218 200 127 2
MspA1I CmG'CkG
Cuts at: 1974 2215 3752 4066 4426 5439 5684 1974
Size: 241 1537 314 360 1013 245 2931
Fragments arranged by size:
2931 1537 1013 360 314 245 241
NaeI GCC'GGC
Cuts at: 4822 4822
Size: 6641
NarI GG'CG_CC
Cuts at: 4072 4320 4072
Size: 248 6393
NcoI C'CATG_G
Cuts at: 107 2381 107
Size: 2274 4367
NgoMIV G'CCGG_C
Cuts at: 4820 4820
Size: 6641
NsiI A_TGCA'T
Cuts at: 210 210
Size: 6641
NspI r_CATG'y
Cuts at: 407 1822 3541 3694 4194 5101 407
Size: 1415 1719 153 500 907 1947
Fragments arranged by size:
1947 1719 1415 907 500 153
PciI A'CATG_T
Cuts at: 5097 5097
Size: 6641
PflMI CCAn_nnn'nTGG
Cuts at: 1417 3340 1417
Size: 1923 4718
PmeI GTTT'AAAC
Cuts at: 3640 3640
Size: 6641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5780 5812 5780
Size: 32 6609
PpuMI rG'GwC_Cy
Cuts at: 2773 2773
Size: 6641
PshAI GACnn'nnGTC
Cuts at: 710 3263 710
Size: 2553 4088
PsiI TTA'TAA
Cuts at: 2168 2524 2168
Size: 356 6285
PstI C_TGCA'G
Cuts at: 2900 3688 4373 2900
Size: 788 685 5168
Fragments arranged by size:
5168 788 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6641
PvuII CAG'CTG
Cuts at: 2215 4066 4426 2215
Size: 1851 360 4430
Fragments arranged by size:
4430 1851 360
RsrII CG'GwC_CG
Cuts at: 4836 4836
Size: 6641
SacI G_AGCT'C
Cuts at: 3655 3655
Size: 6641
SalI G'TCGA_C
Cuts at: 3678 3678
Size: 6641
SapI GCTCTTCn'nnn_
Cuts at: 4664 4874 4664
Size: 210 6431
SbfI CC_TGCA'GG
Cuts at: 3688 3688
Size: 6641
ScaI AGT'ACT
Cuts at: 6158 6158
Size: 6641
SexAI A'CCwGG_T
Cuts at: 3300 3300
Size: 6641
SfcI C'TryA_G
Cuts at: 33 326 2210 2354 2896 3684 4369 5362 5553
Size: 293 1884 144 542 788 685 993 191
Cuts at: 5553 33
Size: 1121
Fragments arranged by size:
1884 1121 993 788 685 542 293 191
144
SfoI GGC'GCC
Cuts at: 4073 4321 4073
Size: 248 6393
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6641
SmaI CCC'GGG
Cuts at: 3663 3663
Size: 6641
SmlI C'TyrA_G
Cuts at: 922 1827 2845 3199 3796 4711 5203 5465 922
Size: 905 1018 354 597 915 492 262 2098
Fragments arranged by size:
2098 1018 915 905 597 492 354 262
SnaBI TAC'GTA
Cuts at: 2974 2974
Size: 6641
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6641
SphI G_CATG'C
Cuts at: 3541 3694 4194 3541
Size: 153 500 5988
Fragments arranged by size:
5988 500 153
SspI AAT'ATT
Cuts at: 966 966
Size: 6641
StyI C'CwwG_G
Cuts at: 107 597 1858 2000 2381 2944 3775 4129 107
Size: 490 1261 142 381 563 831 354 2619
Fragments arranged by size:
2619 1261 831 563 490 381 354 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2415 4224 4357 4816 5025 2415
Size: 1809 133 459 209 4031
Fragments arranged by size:
4031 1809 459 209 133
TatI w'GTAC_w
Cuts at: 382 1043 2408 3509 6156 382
Size: 661 1365 1101 2647 867
Fragments arranged by size:
2647 1365 1101 867 661
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1855 1887 6158 6190 1855
Size: 32 4271 32 2306
Fragments arranged by size:
4271 2306 32 32
Tth111I GACn'n_nGTC
Cuts at: 1372 4438 1372
Size: 3066 3575
XbaI T'CTAG_A
Cuts at: 3672 3672
Size: 6641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1905 3337 4136 1905
Size: 1432 799 4410
Fragments arranged by size:
4410 1432 799
XmaI C'CCGG_G
Cuts at: 3661 3661
Size: 6641
XmnI GAAnn'nnTTC
Cuts at: 1425 1425
Size: 6641
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BaeI
BbvCI BcgI BglI BmgBI BmtI Bpu10I BsiWI BssHII
BstEII ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI PacI PasI PfoI
PmlI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SrfI StuI SwaI XhoI ZraI