Restriction map for
pF1KA1361
(Circular) (Six-base) MAPSORT of: pF1KA1361.seq Check: 5074 from: 1 to: 6116
pF1KA1361 6116 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 648 2345 648
Size: 1697 4419
AatII G_ACGT'C
Cuts at: 1926 1926
Size: 6116
AccI GT'mk_AC
Cuts at: 304 3154 304
Size: 2850 3266
Acc65I G'GTAC_C
Cuts at: 2967 3132 2967
Size: 165 5951
AclI AA'CG_TT
Cuts at: 5956 5956
Size: 6116
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 499 2933 3941 4373 5120 5388 157
Size: 342 2434 1008 432 747 268 885
Fragments arranged by size:
2434 1008 885 747 432 342 268
AflIII A'CryG_T
Cuts at: 748 3098 3468 4572 5549 748
Size: 2350 370 1104 977 1315
Fragments arranged by size:
2350 1315 1104 977 370
AgeI A'CCGG_T
Cuts at: 4504 4504
Size: 6116
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2081 2113 2081
Size: 32 6084
AlwNI CAG_nnn'CTG
Cuts at: 2532 3012 3028 3421 4988 2532
Size: 480 16 393 1567 3660
Fragments arranged by size:
3660 1567 480 393 16
ApaLI G'TGCA_C
Cuts at: 4886 4886
Size: 6116
ApoI r'AATT_y
Cuts at: 1711 3120 5469 5725 1711
Size: 1409 2349 256 2102
Fragments arranged by size:
2349 2102 1409 256
AseI AT'TA_AT
Cuts at: 20 2444 20
Size: 2424 3692
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6116
AvaI C'yCGr_G
Cuts at: 1762 2820 2972 3136 1762
Size: 1058 152 164 4742
Fragments arranged by size:
4742 1058 164 152
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1815 1848 1815
Size: 33 6083
BamHI G'GATC_C
Cuts at: 3141 3141
Size: 6116
BanI G'GyrC_C
Cuts at: 2967 3132 3546 3794 3829 2967
Size: 165 414 248 35 5254
Fragments arranged by size:
5254 414 248 165 35
BanII G_rGCy'C
Cuts at: 161 2165 3130 4160 161
Size: 2004 965 1030 2117
Fragments arranged by size:
2117 2004 1030 965
BbeI G_GCGC'C
Cuts at: 3550 3798 3550
Size: 248 5868
BbsI GAAGACnn'nnnn_
Cuts at: 1210 1210
Size: 6116
BbvCI CC'TCA_GC
Cuts at: 2576 2576
Size: 6116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1440 1474 90
Size: 34 1316 34 4732
Fragments arranged by size:
4732 1316 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2283 4014 4775 6116 2283
Size: 1731 761 1341 2283
Fragments arranged by size:
2283 1731 1341 761
BclI T'GATC_A
Cuts at: 2138 2433 3636 2138
Size: 295 1203 4618
Fragments arranged by size:
4618 1203 295
BglII A'GATC_T
Cuts at: 195 981 1602 3631 5668 195
Size: 786 621 2029 2037 643
Fragments arranged by size:
2037 2029 786 643 621
BlpI GC'TnA_GC
Cuts at: 2423 3228 2423
Size: 805 5311
Bme1580I G_kGCm'C
Cuts at: 2637 3741 3834 4890 2637
Size: 1104 93 1056 3863
Fragments arranged by size:
3863 1104 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2861 2880 3328 3409 3744 5888 2861
Size: 19 448 81 335 2144 3089
Fragments arranged by size:
3089 2144 448 335 81 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1494 4483 1494
Size: 2989 3127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 2113 2362 2554 877
Size: 1236 249 192 4439
Fragments arranged by size:
4439 1236 249 192
Bpu10I CC'TnA_GC
Cuts at: 2576 2576
Size: 6116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 2371 3292 4171 4663 4961 890
Size: 1481 921 879 492 298 2045
Fragments arranged by size:
2045 1481 921 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 740 1259 2625 5820 740
Size: 519 1366 3195 1036
Fragments arranged by size:
3195 1366 1036 519
BsaAI yAC'GTr
Cuts at: 397 3469 397
Size: 3072 3044
BsaBI GATnn'nnATC
Cuts at: 6 1580 3630 5667 6
Size: 1574 2050 2037 455
Fragments arranged by size:
2050 2037 1574 455
BsaHI Gr'CG_yC
Cuts at: 1923 1983 3547 3795 4497 5993 1923
Size: 60 1564 248 702 1496 2046
Fragments arranged by size:
2046 1564 1496 702 248 60
BsaWI w'CCGG_w
Cuts at: 2627 3305 3826 4504 4778 4925 5336 2627
Size: 678 521 678 274 147 411 3407
Fragments arranged by size:
3407 678 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1825 2029 1825
Size: 204 5912
BseYI C'CCAG_C
Cuts at: 1171 2930 3541 4299 4876 5372 1171
Size: 1759 611 758 577 496 1915
Fragments arranged by size:
1915 1759 758 611 577 496
BsiEI CG_ry'CG
Cuts at: 104 3658 3704 4912 104
Size: 3554 46 1208 1308
Fragments arranged by size:
3554 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 161 513 2165 2366 3130 3908 4098 4890 161
Size: 352 1652 201 764 778 190 792 1387
Fragments arranged by size:
1652 1387 792 778 764 352 201 190
BsmI GAATG_Cn'
Cuts at: 798 1954 2550 2803 798
Size: 1156 596 253 4111
Fragments arranged by size:
4111 1156 596 253
Bsp1286I G_dGCh'C
Cuts at: 161 513 2165 2366 2637 3130 3741 3834 3908
Size: 352 1652 201 271 493 611 93 74
Cuts at: 3908 4098 4160 4890 161
Size: 190 62 730 1387
Fragments arranged by size:
1652 1387 730 611 493 352 271 201
190 93 74 62
BspEI T'CCGG_A
Cuts at: 2627 5336 2627
Size: 2709 3407
BspHI T'CATG_A
Cuts at: 5292 5292
Size: 6116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 648 2302 2345 3166 3380 3682 4063 648
Size: 1654 43 821 214 302 381 2701
Fragments arranged by size:
2701 1654 821 381 302 214 43
BsrBI CCG'CTC
Cuts at: 50 4408 4462 5946 6111 50
Size: 4358 54 1484 165 55
Fragments arranged by size:
4358 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 803 4028 803
Size: 3225 2891
BsrFI r'CCGG_y
Cuts at: 4114 4295 4504 4114
Size: 181 209 5726
Fragments arranged by size:
5726 209 181
BssSI C'ACGA_G
Cuts at: 242 3063 4387 4745 242
Size: 2821 1324 358 1613
Fragments arranged by size:
2821 1613 1324 358
BstAPI GCAn_nnn'nTGC
Cuts at: 640 802 2058 3493 640
Size: 162 1256 1435 3263
Fragments arranged by size:
3263 1435 1256 162
BstBI TT'CG_AA
Cuts at: 1791 4477 1791
Size: 2686 3430
BstEII G'GTnAC_C
Cuts at: 2941 2941
Size: 6116
BstXI CCAn_nnnn'nTGG
Cuts at: 264 2903 264
Size: 2639 3477
BstYI r'GATC_y
Cuts at: 1 174 195 436 981 1338 1359 1602 3141
Size: 173 21 241 545 357 21 243 1539
Cuts at: 3141 3608 3631 3966 4212 5224 5310 5322 5668
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5668 1
Size: 449
Fragments arranged by size:
1539 1012 545 467 449 357 346 335
246 243 241 173 86 23 21 21
12
Bsu36I CC'TnA_GG
Cuts at: 2798 2798
Size: 6116
BtgI C'CryG_G
Cuts at: 213 629 695 2882 2935 213
Size: 416 66 2187 53 3394
Fragments arranged by size:
3394 2187 416 66 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1567 4066 4247 4410 1567
Size: 2499 181 163 3273
Fragments arranged by size:
3273 2499 181 163
BtsI GCAGTG_nn'
Cuts at: 236 3359 236
Size: 3123 2993
DraI TTT'AAA
Cuts at: 935 1304 3115 935
Size: 369 1811 3936
Fragments arranged by size:
3936 1811 369
DraIII CAC_nnn'GTG
Cuts at: 508 1275 508
Size: 767 5349
DrdI GACnn_nn'nnGTC
Cuts at: 3822 4680 3822
Size: 858 5258
EaeI y'GGCC_r
Cuts at: 210 269 1125 3701 3875 4266 4293 5638 210
Size: 59 856 2576 174 391 27 1345 688
Fragments arranged by size:
2576 1345 856 688 391 174 59 27
EagI C'GGCC_G
Cuts at: 3701 3701
Size: 6116
EarI CTCTTCn'nnn_
Cuts at: 166 193 518 1097 1325 1697 2187 2654 4139
Size: 27 325 579 228 372 490 467 1485
Cuts at: 4139 4349 166
Size: 210 1933
Fragments arranged by size:
1933 1485 579 490 467 372 325 228
210 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2089 3033 4324 4634 4780 5926 2089
Size: 944 1291 310 146 1146 2279
Fragments arranged by size:
2279 1291 1146 944 310 146
EcoICRI GAG'CTC
Cuts at: 159 2163 3128 159
Size: 2004 965 3147
Fragments arranged by size:
3147 2004 965
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 499 877 2113 2362 2554 2933 3941 4373
Size: 342 378 1236 249 192 379 1008 432
Cuts at: 4373 5120 5388 157
Size: 747 268 885
Fragments arranged by size:
1236 1008 885 747 432 379 378 342
268 249 192
EcoNI CCTnn'n_nnAGG
Cuts at: 1057 1057
Size: 6116
EcoO109I rG'GnC_Cy
Cuts at: 141 2001 2863 3255 141
Size: 1860 862 392 3002
Fragments arranged by size:
3002 1860 862 392
EcoRI G'AATT_C
Cuts at: 3120 3120
Size: 6116
EcoRV GAT'ATC
Cuts at: 561 561
Size: 6116
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1819 1930 4135 1819
Size: 111 2205 3800
Fragments arranged by size:
3800 2205 111
FspI TGC'GCA
Cuts at: 3897 3897
Size: 6116
HaeII r_GCGC'y
Cuts at: 963 3550 3798 4820 5799 963
Size: 2587 248 1022 979 1280
Fragments arranged by size:
2587 1280 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 604 636 718 750 1351 1383 3958 3988 3990
Size: 32 82 32 601 32 2575 30 2
Cuts at: 3990 4020 604
Size: 30 2700
Fragments arranged by size:
2700 2575 601 82 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1212 3155 1212
Size: 1943 4173
HindIII A'AGCT_T
Cuts at: 219 2735 2900 219
Size: 2516 165 3435
Fragments arranged by size:
3435 2516 165
HpaI GTT'AAC
Cuts at: 1212 1212
Size: 6116
KasI G'GCGC_C
Cuts at: 3546 3794 3546
Size: 248 5868
KpnI G_GTAC'C
Cuts at: 2971 3136 2971
Size: 165 5951
MluI A'CGCG_T
Cuts at: 5549 5549
Size: 6116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1131 2091 2690 4787 4971 1131
Size: 960 599 2097 184 2276
Fragments arranged by size:
2276 2097 960 599 184
MscI TGG'CCA
Cuts at: 212 271 3877 212
Size: 59 3606 2451
Fragments arranged by size:
3606 2451 59
MslI CAynn'nnrTG
Cuts at: 1276 4105 4232 1276
Size: 2829 127 3160
Fragments arranged by size:
3160 2829 127
MspA1I CmG'CkG
Cuts at: 2175 2520 3227 3541 3901 4914 5159 2175
Size: 345 707 314 360 1013 245 3132
Fragments arranged by size:
3132 1013 707 360 345 314 245
NaeI GCC'GGC
Cuts at: 4297 4297
Size: 6116
NarI GG'CG_CC
Cuts at: 3547 3795 3547
Size: 248 5868
NcoI C'CATG_G
Cuts at: 213 629 695 2882 2935 213
Size: 416 66 2187 53 3394
Fragments arranged by size:
3394 2187 416 66 53
NgoMIV G'CCGG_C
Cuts at: 4295 4295
Size: 6116
NruI TCG'CGA
Cuts at: 2603 2603
Size: 6116
NspI r_CATG'y
Cuts at: 752 1179 2056 2221 3102 3169 3669 4576 752
Size: 427 877 165 881 67 500 907 2292
Fragments arranged by size:
2292 907 881 877 500 427 165 67
PciI A'CATG_T
Cuts at: 748 3098 4572 748
Size: 2350 1474 2292
Fragments arranged by size:
2350 2292 1474
PfoI T'CCnGG_A
Cuts at: 1058 2859 1058
Size: 1801 4315
PmeI GTTT'AAAC
Cuts at: 3115 3115
Size: 6116
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1571 1603 5255 5287 1571
Size: 32 3652 32 2400
Fragments arranged by size:
3652 2400 32 32
PpuMI rG'GwC_Cy
Cuts at: 141 2001 2863 141
Size: 1860 862 3394
Fragments arranged by size:
3394 1860 862
PspXI vC'TCGA_Gb
Cuts at: 2972 2972
Size: 6116
PstI C_TGCA'G
Cuts at: 1031 1635 2525 3163 3848 1031
Size: 604 890 638 685 3299
Fragments arranged by size:
3299 890 685 638 604
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6116
PvuII CAG'CTG
Cuts at: 2175 2520 3541 3901 2175
Size: 345 1021 360 4390
Fragments arranged by size:
4390 1021 360 345
RsrII CG'GwC_CG
Cuts at: 4311 4311
Size: 6116
SacI G_AGCT'C
Cuts at: 161 2165 3130 161
Size: 2004 965 3147
Fragments arranged by size:
3147 2004 965
SalI G'TCGA_C
Cuts at: 3153 3153
Size: 6116
SapI GCTCTTCn'nnn_
Cuts at: 166 193 518 4139 4349 166
Size: 27 325 3621 210 1933
Fragments arranged by size:
3621 1933 325 210 27
SbfI CC_TGCA'GG
Cuts at: 3163 3163
Size: 6116
ScaI AGT'ACT
Cuts at: 5633 5633
Size: 6116
SfcI C'TryA_G
Cuts at: 33 841 1027 1631 2521 3159 3844 4837 5028
Size: 808 186 604 890 638 685 993 191
Cuts at: 5028 33
Size: 1121
Fragments arranged by size:
1121 993 890 808 685 638 604 191
186
SfoI GGC'GCC
Cuts at: 3548 3796 3548
Size: 248 5868
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6116
SmaI CCC'GGG
Cuts at: 2822 3138 2822
Size: 316 5800
SmlI C'TyrA_G
Cuts at: 905 1762 2386 2972 3271 4186 4678 4940 905
Size: 857 624 586 299 915 492 262 2081
Fragments arranged by size:
2081 915 857 624 586 492 299 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6116
SphI G_CATG'C
Cuts at: 2056 3169 3669 2056
Size: 1113 500 4503
Fragments arranged by size:
4503 1113 500
SspI AAT'ATT
Cuts at: 427 427
Size: 6116
StyI C'CwwG_G
Cuts at: 213 629 695 2882 2935 3250 3604 213
Size: 416 66 2187 53 315 354 2725
Fragments arranged by size:
2725 2187 416 354 315 66 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2875 3699 3832 4291 4500 2875
Size: 824 133 459 209 4491
Fragments arranged by size:
4491 824 459 209 133
TatI w'GTAC_w
Cuts at: 2315 5631 2315
Size: 3316 2800
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2482 2514 5633 5665 2482
Size: 32 3119 32 2933
Fragments arranged by size:
3119 2933 32 32
Tth111I GACn'n_nGTC
Cuts at: 2005 3913 2005
Size: 1908 4208
XbaI T'CTAG_A
Cuts at: 3147 3147
Size: 6116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 220 264 651 2867 2900 3611 220
Size: 44 387 2216 33 711 2725
Fragments arranged by size:
2725 2216 711 387 44 33
XhoI C'TCGA_G
Cuts at: 1762 2972 1762
Size: 1210 4906
XmaI C'CCGG_G
Cuts at: 2820 3136 2820
Size: 316 5800
XmnI GAAnn'nnTTC
Cuts at: 187 187
Size: 6116
ZraI GAC'GTC
Cuts at: 1924 1924
Size: 6116
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PfoI PmeI
PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI ApaI AscI AvrII
BfrBI BglI BmgBI BmtI BsaXI BsgI BsiWI BsmBI
BsrGI BssHII BstZ17I ClaI CspCI FseI FspAI MfeI
NdeI NheI NotI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI