Restriction map for
pF1KA1372
(Circular) (Six-base) MAPSORT of: pF1KA1372.seq Check: 5533 from: 1 to: 5360
pF1KA1372 5360 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 344 2308 344
Size: 1964 3396
AatII G_ACGT'C
Cuts at: 1781 1781
Size: 5360
AccI GT'mk_AC
Cuts at: 2398 2398
Size: 5360
Acc65I G'GTAC_C
Cuts at: 1970 2376 1970
Size: 406 4954
AclI AA'CG_TT
Cuts at: 5200 5200
Size: 5360
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 652 678 895 3185 3617 4364 4632 190
Size: 462 26 217 2290 432 747 268 918
Fragments arranged by size:
2290 918 747 462 432 268 217 26
AfeI AGC'GCT
Cuts at: 1114 1114
Size: 5360
AflIII A'CryG_T
Cuts at: 1555 2712 3816 4793 1555
Size: 1157 1104 977 2122
Fragments arranged by size:
2122 1157 1104 977
AgeI A'CCGG_T
Cuts at: 3748 3748
Size: 5360
AhdI GACnn_n'nnGTC
Cuts at: 120 120
Size: 5360
AleI CACnn'nnGTG
Cuts at: 1990 1990
Size: 5360
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 799 831 799
Size: 32 5328
AlwNI CAG_nnn'CTG
Cuts at: 537 738 2665 4232 537
Size: 201 1927 1567 1665
Fragments arranged by size:
1927 1665 1567 201
ApaI G_GGCC'C
Cuts at: 2093 2093
Size: 5360
ApaLI G'TGCA_C
Cuts at: 1193 1855 4130 1193
Size: 662 2275 2423
Fragments arranged by size:
2423 2275 662
ApoI r'AATT_y
Cuts at: 2364 4713 4969 2364
Size: 2349 256 2755
Fragments arranged by size:
2755 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5360
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
AvaI C'yCGr_G
Cuts at: 393 453 964 1064 1169 1612 1957 1988 2092
Size: 60 511 100 105 443 345 31 104
Cuts at: 2092 2380 393
Size: 288 3373
Fragments arranged by size:
3373 511 443 345 288 105 104 100
60 31
BamHI G'GATC_C
Cuts at: 2385 2385
Size: 5360
BanI G'GyrC_C
Cuts at: 847 1757 1970 2376 2790 3038 3073 847
Size: 910 213 406 414 248 35 3134
Fragments arranged by size:
3134 910 414 406 248 213 35
BanII G_rGCy'C
Cuts at: 974 1138 1573 1958 2093 2110 2132 2374 3404
Size: 164 435 385 135 17 22 242 1030
Cuts at: 3404 974
Size: 2930
Fragments arranged by size:
2930 1030 435 385 242 164 135 22
17
BbeI G_GCGC'C
Cuts at: 1761 2794 3042 1761
Size: 1033 248 4079
Fragments arranged by size:
4079 1033 248
BbsI GAAGACnn'nnnn_
Cuts at: 1163 1505 1862 2279 2343 1163
Size: 342 357 417 64 4180
Fragments arranged by size:
4180 417 357 342 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 189 223 1636 1670 189
Size: 34 1413 34 3879
Fragments arranged by size:
3879 1413 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3258 4019 5360 3258
Size: 761 1341 3258
Fragments arranged by size:
3258 1341 761
BclI T'GATC_A
Cuts at: 2880 2880
Size: 5360
BglI GCCn_nnn'nGGC
Cuts at: 1133 1133
Size: 5360
BglII A'GATC_T
Cuts at: 2875 4912 2875
Size: 2037 3323
BlpI GC'TnA_GC
Cuts at: 1481 1690 2472 1481
Size: 209 782 4369
Fragments arranged by size:
4369 782 209
Bme1580I G_kGCm'C
Cuts at: 919 1197 1859 2093 2985 3078 4134 919
Size: 278 662 234 892 93 1056 2145
Fragments arranged by size:
2145 1056 892 662 278 234 93
BmrI ACTGGGnnnn_n'
Cuts at: 126 2262 2572 2653 2988 5132 126
Size: 2136 310 81 335 2144 354
Fragments arranged by size:
2144 2136 354 335 310 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 744 2124 3727 744
Size: 1380 1603 2377
Fragments arranged by size:
2377 1603 1380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 1883 2282 430
Size: 1453 399 3508
Fragments arranged by size:
3508 1453 399
Bpu10I CC'TnA_GC
Cuts at: 1109 1109
Size: 5360
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 922 2536 3415 3907 4205 274
Size: 648 1614 879 492 298 1429
Fragments arranged by size:
1614 1429 879 648 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 631 5064 631
Size: 4433 927
BsaAI yAC'GTr
Cuts at: 2713 2713
Size: 5360
BsaBI GATnn'nnATC
Cuts at: 6 2874 4911 6
Size: 2868 2037 455
Fragments arranged by size:
2868 2037 455
BsaHI Gr'CG_yC
Cuts at: 685 829 1758 1778 2791 3039 3741 5237 685
Size: 144 929 20 1013 248 702 1496 808
Fragments arranged by size:
1496 1013 929 808 702 248 144 20
BsaWI w'CCGG_w
Cuts at: 446 2120 2549 3070 3748 4022 4169 4580 446
Size: 1674 429 521 678 274 147 411 1226
Fragments arranged by size:
1674 1226 678 521 429 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 799 829 1045 1075 2014 2044 799
Size: 30 216 30 939 30 4115
Fragments arranged by size:
4115 939 216 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 331 919 982 1050 1084 1094 1369 1399 1402
Size: 588 63 68 34 10 275 30 3
Cuts at: 1402 1441 1444 331
Size: 39 3 4247
Fragments arranged by size:
4247 588 275 68 63 39 34 30
10 3 3
BseYI C'CCAG_C
Cuts at: 537 851 2785 3543 4120 4616 537
Size: 314 1934 758 577 496 1281
Fragments arranged by size:
1934 1281 758 577 496 314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 2122 2216 880
Size: 1242 94 4024
Fragments arranged by size:
4024 1242 94
BsiEI CG_ry'CG
Cuts at: 104 2902 2948 4156 104
Size: 2798 46 1208 1308
Fragments arranged by size:
2798 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 290 710 1197 1859 2374 3152 3342 4134 290
Size: 420 487 662 515 778 190 792 1516
Fragments arranged by size:
1516 792 778 662 515 487 420 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1062 1785 1062
Size: 723 4637
Bsp1286I G_dGCh'C
Cuts at: 290 710 919 974 1138 1197 1573 1859 1958
Size: 420 209 55 164 59 376 286 99
Cuts at: 1958 2093 2110 2132 2374 2985 3078 3152 3342
Size: 135 17 22 242 611 93 74 190
Cuts at: 3342 3404 4134 290
Size: 62 730 1516
Fragments arranged by size:
1516 730 611 420 376 286 242 209
190 164 135 99 93 74 62 59
55 22 17
BspEI T'CCGG_A
Cuts at: 2120 4580 2120
Size: 2460 2900
BspHI T'CATG_A
Cuts at: 4536 4536
Size: 5360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 344 890 1751 2308 2410 2624 2926 3307 344
Size: 546 861 557 102 214 302 381 2397
Fragments arranged by size:
2397 861 557 546 381 302 214 102
BsrBI CCG'CTC
Cuts at: 50 1513 1551 1577 3652 3706 5190 5355 50
Size: 1463 38 26 2075 54 1484 165 55
Fragments arranged by size:
2075 1484 1463 165 55 54 38 26
BsrDI GCAATG_nn'
Cuts at: 158 2331 3272 158
Size: 2173 941 2246
Fragments arranged by size:
2246 2173 941
BsrFI r'CCGG_y
Cuts at: 2069 3358 3539 3748 2069
Size: 1289 181 209 3681
Fragments arranged by size:
3681 1289 209 181
BsrGI T'GTAC_A
Cuts at: 1271 1271
Size: 5360
BssSI C'ACGA_G
Cuts at: 3631 3989 3631
Size: 358 5002
BstAPI GCAn_nnn'nTGC
Cuts at: 647 2737 647
Size: 2090 3270
BstBI TT'CG_AA
Cuts at: 2009 3721 2009
Size: 1712 3648
BstXI CCAn_nnnn'nTGG
Cuts at: 408 408
Size: 5360
BstYI r'GATC_y
Cuts at: 1 465 923 1031 1766 2385 2852 2875 3210
Size: 464 458 108 735 619 467 23 335
Cuts at: 3210 3456 4468 4554 4566 4912 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 735 619 467 464 458 449 346
335 246 108 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2042 2042
Size: 5360
BtgI C'CryG_G
Cuts at: 359 1821 359
Size: 1462 3898
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3310 3491 3654 3310
Size: 181 163 5016
Fragments arranged by size:
5016 181 163
BtsI GCAGTG_nn'
Cuts at: 163 432 760 1236 1720 1851 2603 163
Size: 269 328 476 484 131 752 2920
Fragments arranged by size:
2920 752 484 476 328 269 131
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 977 1012 977
Size: 35 5325
DraI TTT'AAA
Cuts at: 2359 2359
Size: 5360
DrdI GACnn_nn'nnGTC
Cuts at: 3066 3924 3066
Size: 858 4502
EaeI y'GGCC_r
Cuts at: 599 1818 1824 2945 3119 3510 3537 4882 599
Size: 1219 6 1121 174 391 27 1345 1077
Fragments arranged by size:
1345 1219 1121 1077 391 174 27 6
EagI C'GGCC_G
Cuts at: 2945 2945
Size: 5360
EarI CTCTTCn'nnn_
Cuts at: 559 896 1433 1442 2007 2115 3383 3593 559
Size: 337 537 9 565 108 1268 210 2326
Fragments arranged by size:
2326 1268 565 537 337 210 108 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 436 950 1789 3568 3878 4024 5170 436
Size: 514 839 1779 310 146 1146 626
Fragments arranged by size:
1779 1146 839 626 514 310 146
EcoICRI GAG'CTC
Cuts at: 2372 2372
Size: 5360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 430 652 678 895 1883 2282 3185 3617
Size: 240 222 26 217 988 399 903 432
Cuts at: 3617 4364 4632 190
Size: 747 268 918
Fragments arranged by size:
988 918 903 747 432 399 268 240
222 217 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2140 2140
Size: 5360
EcoO109I rG'GnC_Cy
Cuts at: 174 498 1216 1517 2089 2499 174
Size: 324 718 301 572 410 3035
Fragments arranged by size:
3035 718 572 410 324 301
EcoRI G'AATT_C
Cuts at: 2364 2364
Size: 5360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3379 3379
Size: 5360
FspI TGC'GCA
Cuts at: 1747 3141 1747
Size: 1394 3966
HaeII r_GCGC'y
Cuts at: 1116 1761 1913 2794 3042 4064 5043 1116
Size: 645 152 881 248 1022 979 1433
Fragments arranged by size:
1433 1022 979 881 645 248 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 138 376 408 1011 1023 1043 1055 1765
Size: 32 238 32 603 12 20 12 710
Cuts at: 1765 1797 2278 2310 3202 3232 3234 3264 106
Size: 32 481 32 892 30 2 30 2202
Fragments arranged by size:
2202 892 710 603 481 238 32 32
32 32 30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 657 1596 2399 657
Size: 939 803 3618
Fragments arranged by size:
3618 939 803
HpaI GTT'AAC
Cuts at: 1596 1596
Size: 5360
KasI G'GCGC_C
Cuts at: 1757 2790 3038 1757
Size: 1033 248 4079
Fragments arranged by size:
4079 1033 248
KpnI G_GTAC'C
Cuts at: 1974 2380 1974
Size: 406 4954
MluI A'CGCG_T
Cuts at: 4793 4793
Size: 5360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4031 4215 4031
Size: 184 5176
MscI TGG'CCA
Cuts at: 601 1826 3121 601
Size: 1225 1295 2840
Fragments arranged by size:
2840 1295 1225
MslI CAynn'nnrTG
Cuts at: 1526 1990 3349 3476 1526
Size: 464 1359 127 3410
Fragments arranged by size:
3410 1359 464 127
MspA1I CmG'CkG
Cuts at: 404 417 534 786 2076 2471 2785 3145 4158
Size: 13 117 252 1290 395 314 360 1013
Cuts at: 4158 4403 404
Size: 245 1361
Fragments arranged by size:
1361 1290 1013 395 360 314 252 245
117 13
NaeI GCC'GGC
Cuts at: 3541 3541
Size: 5360
NarI GG'CG_CC
Cuts at: 1758 2791 3039 1758
Size: 1033 248 4079
Fragments arranged by size:
4079 1033 248
NcoI C'CATG_G
Cuts at: 359 359
Size: 5360
NgoMIV G'CCGG_C
Cuts at: 3539 3539
Size: 5360
NspI r_CATG'y
Cuts at: 300 650 1559 2413 2913 3820 300
Size: 350 909 854 500 907 1840
Fragments arranged by size:
1840 909 907 854 500 350
PasI CC'CwG_GG
Cuts at: 132 2081 132
Size: 1949 3411
PciI A'CATG_T
Cuts at: 1555 3816 1555
Size: 2261 3099
PflMI CCAn_nnn'nTGG
Cuts at: 438 1813 2346 438
Size: 1375 533 3452
Fragments arranged by size:
3452 1375 533
PfoI T'CCnGG_A
Cuts at: 461 823 1583 1769 461
Size: 362 760 186 4052
Fragments arranged by size:
4052 760 362 186
PmeI GTTT'AAAC
Cuts at: 2359 2359
Size: 5360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 799 831 4499 4531 799
Size: 32 3668 32 1628
Fragments arranged by size:
3668 1628 32 32
PpuMI rG'GwC_Cy
Cuts at: 498 1216 1517 498
Size: 718 301 4341
Fragments arranged by size:
4341 718 301
PspOMI G'GGCC_C
Cuts at: 2089 2089
Size: 5360
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1954 1986 1954
Size: 32 5328
PstI C_TGCA'G
Cuts at: 755 1667 1846 2319 2407 3092 755
Size: 912 179 473 88 685 3023
Fragments arranged by size:
3023 912 685 473 179 88
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5360
PvuII CAG'CTG
Cuts at: 404 417 534 2785 3145 404
Size: 13 117 2251 360 2619
Fragments arranged by size:
2619 2251 360 117 13
RsrII CG'GwC_CG
Cuts at: 3555 3555
Size: 5360
SacI G_AGCT'C
Cuts at: 2374 2374
Size: 5360
SalI G'TCGA_C
Cuts at: 2397 2397
Size: 5360
SanDI GG'GwC_CC
Cuts at: 1517 1517
Size: 5360
SapI GCTCTTCn'nnn_
Cuts at: 559 2115 3383 3593 559
Size: 1556 1268 210 2326
Fragments arranged by size:
2326 1556 1268 210
SbfI CC_TGCA'GG
Cuts at: 1667 2319 2407 1667
Size: 652 88 4620
Fragments arranged by size:
4620 652 88
ScaI AGT'ACT
Cuts at: 4877 4877
Size: 5360
SexAI A'CCwGG_T
Cuts at: 1589 1589
Size: 5360
SfcI C'TryA_G
Cuts at: 33 751 1663 1842 2185 2315 2403 3088 4081
Size: 718 912 179 343 130 88 685 993
Cuts at: 4081 4272 33
Size: 191 1121
Fragments arranged by size:
1121 993 912 718 685 343 191 179
130 88
SfoI GGC'GCC
Cuts at: 1759 2792 3040 1759
Size: 1033 248 4079
Fragments arranged by size:
4079 1033 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
SmaI CCC'GGG
Cuts at: 455 1066 1171 2382 455
Size: 611 105 1211 3433
Fragments arranged by size:
3433 1211 611 105
SmlI C'TyrA_G
Cuts at: 289 937 2515 3430 3922 4184 289
Size: 648 1578 915 492 262 1465
Fragments arranged by size:
1578 1465 915 648 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5360
SphI G_CATG'C
Cuts at: 2413 2913 2413
Size: 500 4860
SspI AAT'ATT
Cuts at: 144 144
Size: 5360
StuI AGG'CCT
Cuts at: 673 1075 1283 1831 673
Size: 402 208 548 4202
Fragments arranged by size:
4202 548 402 208
StyI C'CwwG_G
Cuts at: 359 1292 1695 2494 2848 359
Size: 933 403 799 354 2871
Fragments arranged by size:
2871 933 799 403 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 108 865 2943 3076 3535 3744 108
Size: 757 2078 133 459 209 1724
Fragments arranged by size:
2078 1724 757 459 209 133
TatI w'GTAC_w
Cuts at: 293 1271 4875 293
Size: 978 3604 778
Fragments arranged by size:
3604 978 778
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4877 4909 4877
Size: 32 5328
Tth111I GACn'n_nGTC
Cuts at: 3157 3157
Size: 5360
XbaI T'CTAG_A
Cuts at: 2113 2391 2113
Size: 278 5082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2855 2855
Size: 5360
XmaI C'CCGG_G
Cuts at: 453 1064 1169 2380 453
Size: 611 105 1211 3433
Fragments arranged by size:
3433 1211 611 105
XmnI GAAnn'nnTTC
Cuts at: 238 238
Size: 5360
ZraI GAC'GTC
Cuts at: 1779 1779
Size: 5360
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AlfI AscI AvrII BaeI BbvCI BfrBI BmgBI
BmtI BsiWI BsmI BssHII BstEII BstZ17I ClaI DraIII
EcoRV FseI FspAI HindIII MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI SacII
SfiI SgrAI SnaBI SrfI SwaI XhoI