Restriction map for
pF1KA1376
(Circular) (Six-base) MAPSORT of: pF1KA1376.seq Check: 7529 from: 1 to: 4355
pF1KA1376 4355 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1393 1393
Size: 4355
Acc65I G'GTAC_C
Cuts at: 1036 1371 1036
Size: 335 4020
AclI AA'CG_TT
Cuts at: 4195 4195
Size: 4355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 1157 2180 2612 3359 3627 356
Size: 801 1023 432 747 268 1084
Fragments arranged by size:
1084 1023 801 747 432 268
AflII C'TTAA_G
Cuts at: 684 684
Size: 4355
AflIII A'CryG_T
Cuts at: 1707 2811 3788 1707
Size: 1104 977 2274
Fragments arranged by size:
2274 1104 977
AgeI A'CCGG_T
Cuts at: 2743 2743
Size: 4355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 458 458
Size: 4355
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 745 777 745
Size: 32 4323
AlwNI CAG_nnn'CTG
Cuts at: 1660 3227 1660
Size: 1567 2788
ApaLI G'TGCA_C
Cuts at: 3125 3125
Size: 4355
ApoI r'AATT_y
Cuts at: 210 251 571 914 1359 3708 3964 210
Size: 41 320 343 445 2349 256 601
Fragments arranged by size:
2349 601 445 343 320 256 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 4355
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
AvaI C'yCGr_G
Cuts at: 1375 1375
Size: 4355
BamHI G'GATC_C
Cuts at: 1380 1380
Size: 4355
BanI G'GyrC_C
Cuts at: 774 1036 1371 1785 2033 2068 774
Size: 262 335 414 248 35 3061
Fragments arranged by size:
3061 414 335 262 248 35
BanII G_rGCy'C
Cuts at: 1230 1369 2399 1230
Size: 139 1030 3186
Fragments arranged by size:
3186 1030 139
BbeI G_GCGC'C
Cuts at: 1789 2037 1789
Size: 248 4107
BciVI GTATCCnnnnn_n'
Cuts at: 183 2253 3014 4355 183
Size: 2070 761 1341 183
Fragments arranged by size:
2070 1341 761 183
BclI T'GATC_A
Cuts at: 1311 1875 1311
Size: 564 3791
BfrBI ATG'CAT
Cuts at: 445 445
Size: 4355
BglI GCCn_nnn'nGGC
Cuts at: 500 500
Size: 4355
BglII A'GATC_T
Cuts at: 1870 3907 1870
Size: 2037 2318
BlpI GC'TnA_GC
Cuts at: 1467 1467
Size: 4355
Bme1580I G_kGCm'C
Cuts at: 380 1980 2073 3129 380
Size: 1600 93 1056 1606
Fragments arranged by size:
1606 1600 1056 93
BmgBI CAC'GTC
Cuts at: 893 893
Size: 4355
BmrI ACTGGGnnnn_n'
Cuts at: 233 1567 1648 1983 4127 233
Size: 1334 81 335 2144 461
Fragments arranged by size:
2144 1334 461 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 774 2722 774
Size: 1948 2407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1016 1016
Size: 4355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1056 1531 2410 2902 3200 1056
Size: 475 879 492 298 2211
Fragments arranged by size:
2211 879 492 475 298
BsaI GGTCTCn'nnnn_
Cuts at: 4059 4059
Size: 4355
BsaAI yAC'GTr
Cuts at: 1708 1708
Size: 4355
BsaBI GATnn'nnATC
Cuts at: 6 1304 1869 3906 6
Size: 1298 565 2037 455
Fragments arranged by size:
2037 1298 565 455
BsaHI Gr'CG_yC
Cuts at: 1786 2034 2736 4232 1786
Size: 248 702 1496 1909
Fragments arranged by size:
1909 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1544 2065 2743 3017 3164 3575 1544
Size: 521 678 274 147 411 2324
Fragments arranged by size:
2324 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 289 319 289
Size: 30 4325
BseYI C'CCAG_C
Cuts at: 114 1147 1780 2538 3115 3611 114
Size: 1033 633 758 577 496 858
Fragments arranged by size:
1033 858 758 633 577 496
BsiEI CG_ry'CG
Cuts at: 104 1897 1943 3151 104
Size: 1793 46 1208 1308
Fragments arranged by size:
1793 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1369 2147 2337 3129 1369
Size: 778 190 792 2595
Fragments arranged by size:
2595 792 778 190
BsmI GAATG_Cn'
Cuts at: 445 445
Size: 4355
BsmBI CGTCTCn'nnnn_
Cuts at: 201 883 201
Size: 682 3673
Bsp1286I G_dGCh'C
Cuts at: 380 1230 1369 1980 2073 2147 2337 2399 3129
Size: 850 139 611 93 74 190 62 730
Cuts at: 3129 380
Size: 1606
Fragments arranged by size:
1606 850 730 611 190 139 93 74
62
BspEI T'CCGG_A
Cuts at: 3575 3575
Size: 4355
BspHI T'CATG_A
Cuts at: 3531 3531
Size: 4355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1405 1619 1921 2302 1405
Size: 214 302 381 3458
Fragments arranged by size:
3458 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2647 2701 4185 4350 50
Size: 2597 54 1484 165 55
Fragments arranged by size:
2597 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 171 2267 171
Size: 2096 2259
BsrFI r'CCGG_y
Cuts at: 301 2353 2534 2743 301
Size: 2052 181 209 1913
Fragments arranged by size:
2052 1913 209 181
BssSI C'ACGA_G
Cuts at: 1346 2626 2984 1346
Size: 1280 358 2717
Fragments arranged by size:
2717 1280 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1732 1732
Size: 4355
BstBI TT'CG_AA
Cuts at: 488 2716 488
Size: 2228 2127
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4355
BstYI r'GATC_y
Cuts at: 1 1380 1847 1870 2205 2451 3463 3549 3561
Size: 1379 467 23 335 246 1012 86 12
Cuts at: 3561 3907 1
Size: 346 449
Fragments arranged by size:
1379 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 669 669
Size: 4355
BtgI C'CryG_G
Cuts at: 107 107
Size: 4355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2305 2486 2649 2305
Size: 181 163 4011
Fragments arranged by size:
4011 181 163
BtsI GCAGTG_nn'
Cuts at: 510 1598 510
Size: 1088 3267
DraI TTT'AAA
Cuts at: 1354 1354
Size: 4355
DrdI GACnn_nn'nnGTC
Cuts at: 166 2061 2919 166
Size: 1895 858 1602
Fragments arranged by size:
1895 1602 858
EaeI y'GGCC_r
Cuts at: 1940 2114 2505 2532 3877 1940
Size: 174 391 27 1345 2418
Fragments arranged by size:
2418 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1940 1940
Size: 4355
EarI CTCTTCn'nnn_
Cuts at: 121 765 2378 2588 121
Size: 644 1613 210 1888
Fragments arranged by size:
1888 1613 644 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1197 2563 2873 3019 4165 1197
Size: 1366 310 146 1146 1387
Fragments arranged by size:
1387 1366 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1367 1367
Size: 4355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 1016 1157 2180 2612 3359 3627 356
Size: 660 141 1023 432 747 268 1084
Fragments arranged by size:
1084 1023 747 660 432 268 141
EcoO109I rG'GnC_Cy
Cuts at: 1494 1494
Size: 4355
EcoRI G'AATT_C
Cuts at: 1359 1359
Size: 4355
EcoRV GAT'ATC
Cuts at: 597 597
Size: 4355
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4355
FspI TGC'GCA
Cuts at: 2136 2136
Size: 4355
HaeII r_GCGC'y
Cuts at: 1789 2037 3059 4038 1789
Size: 248 1022 979 2106
Fragments arranged by size:
2106 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 181 213 1318 1350 2197 2227 2229 2259 181
Size: 32 1105 32 847 30 2 30 2277
Fragments arranged by size:
2277 1105 847 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1394 1394
Size: 4355
HindIII A'AGCT_T
Cuts at: 141 141
Size: 4355
KasI G'GCGC_C
Cuts at: 1785 2033 1785
Size: 248 4107
KpnI G_GTAC'C
Cuts at: 1040 1375 1040
Size: 335 4020
MluI A'CGCG_T
Cuts at: 3788 3788
Size: 4355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3026 3210 3026
Size: 184 4171
MscI TGG'CCA
Cuts at: 2116 2116
Size: 4355
MslI CAynn'nnrTG
Cuts at: 440 503 1099 2344 2471 440
Size: 63 596 1245 127 2324
Fragments arranged by size:
2324 1245 596 127 63
MspA1I CmG'CkG
Cuts at: 1466 1780 2140 3153 3398 1466
Size: 314 360 1013 245 2423
Fragments arranged by size:
2423 1013 360 314 245
NaeI GCC'GGC
Cuts at: 303 2536 303
Size: 2233 2122
NarI GG'CG_CC
Cuts at: 1786 2034 1786
Size: 248 4107
NcoI C'CATG_G
Cuts at: 107 107
Size: 4355
NgoMIV G'CCGG_C
Cuts at: 301 2534 301
Size: 2233 2122
NsiI A_TGCA'T
Cuts at: 447 447
Size: 4355
NspI r_CATG'y
Cuts at: 271 1408 1908 2815 271
Size: 1137 500 907 1811
Fragments arranged by size:
1811 1137 907 500
PciI A'CATG_T
Cuts at: 2811 2811
Size: 4355
PfoI T'CCnGG_A
Cuts at: 993 1256 993
Size: 263 4092
PmeI GTTT'AAAC
Cuts at: 1354 1354
Size: 4355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 4323
PstI C_TGCA'G
Cuts at: 1402 2087 1402
Size: 685 3670
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4355
PvuII CAG'CTG
Cuts at: 1780 2140 1780
Size: 360 3995
RsrII CG'GwC_CG
Cuts at: 2550 2550
Size: 4355
SacI G_AGCT'C
Cuts at: 1369 1369
Size: 4355
SalI G'TCGA_C
Cuts at: 1392 1392
Size: 4355
SapI GCTCTTCn'nnn_
Cuts at: 765 2378 2588 765
Size: 1613 210 2532
Fragments arranged by size:
2532 1613 210
SbfI CC_TGCA'GG
Cuts at: 1402 1402
Size: 4355
ScaI AGT'ACT
Cuts at: 3872 3872
Size: 4355
SfcI C'TryA_G
Cuts at: 33 945 1398 2083 3076 3267 33
Size: 912 453 685 993 191 1121
Fragments arranged by size:
1121 993 912 685 453 191
SfoI GGC'GCC
Cuts at: 1787 2035 1787
Size: 248 4107
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
SmaI CCC'GGG
Cuts at: 1377 1377
Size: 4355
SmlI C'TyrA_G
Cuts at: 684 1071 1510 2425 2917 3179 684
Size: 387 439 915 492 262 1860
Fragments arranged by size:
1860 915 492 439 387 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4355
SphI G_CATG'C
Cuts at: 1408 1908 1408
Size: 500 3855
SspI AAT'ATT
Cuts at: 967 967
Size: 4355
StuI AGG'CCT
Cuts at: 540 805 540
Size: 265 4090
StyI C'CwwG_G
Cuts at: 107 541 1489 1843 107
Size: 434 948 354 2619
Fragments arranged by size:
2619 948 434 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 507 1161 1938 2071 2530 2739 507
Size: 654 777 133 459 209 2123
Fragments arranged by size:
2123 777 654 459 209 133
TatI w'GTAC_w
Cuts at: 3870 3870
Size: 4355
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3872 3904 3872
Size: 32 4323
Tth111I GACn'n_nGTC
Cuts at: 2152 2152
Size: 4355
XbaI T'CTAG_A
Cuts at: 292 1386 292
Size: 1094 3261
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1850 1850
Size: 4355
XmaI C'CCGG_G
Cuts at: 1375 1375
Size: 4355
XmnI GAAnn'nnTTC
Cuts at: 728 728
Size: 4355
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BmtI Bpu10I BseRI BsgI
BsiWI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoNI FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI