Restriction map for
pF1KA1378
(Circular) (Six-base) MAPSORT of: pF1KA1378.seq Check: 1705 from: 1 to: 4973
pF1KA1378 4973 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2011 2011
Size: 4973
Acc65I G'GTAC_C
Cuts at: 1989 1989
Size: 4973
AclI AA'CG_TT
Cuts at: 4813 4813
Size: 4973
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 431 719 2798 3230 3977 4245 98
Size: 333 288 2079 432 747 268 826
Fragments arranged by size:
2079 826 747 432 333 288 268
AflIII A'CryG_T
Cuts at: 1179 2325 3429 4406 1179
Size: 1146 1104 977 1746
Fragments arranged by size:
1746 1146 1104 977
AgeI A'CCGG_T
Cuts at: 3361 3361
Size: 4973
AlwNI CAG_nnn'CTG
Cuts at: 1012 2278 3845 1012
Size: 1266 1567 2140
Fragments arranged by size:
2140 1567 1266
ApaLI G'TGCA_C
Cuts at: 3743 3743
Size: 4973
ApoI r'AATT_y
Cuts at: 1922 1977 4326 4582 1922
Size: 55 2349 256 2313
Fragments arranged by size:
2349 2313 256 55
AseI AT'TA_AT
Cuts at: 20 20
Size: 4973
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4973
AvaI C'yCGr_G
Cuts at: 1993 1993
Size: 4973
BamHI G'GATC_C
Cuts at: 1942 1998 1942
Size: 56 4917
BanI G'GyrC_C
Cuts at: 1437 1989 2403 2651 2686 1437
Size: 552 414 248 35 3724
Fragments arranged by size:
3724 552 414 248 35
BanII G_rGCy'C
Cuts at: 1987 3017 1987
Size: 1030 3943
BbeI G_GCGC'C
Cuts at: 2407 2655 2407
Size: 248 4725
BbsI GAAGACnn'nnnn_
Cuts at: 470 722 470
Size: 252 4721
BbvCI CC'TCA_GC
Cuts at: 833 833
Size: 4973
BciVI GTATCCnnnnn_n'
Cuts at: 2871 3632 4973 2871
Size: 761 1341 2871
Fragments arranged by size:
2871 1341 761
BclI T'GATC_A
Cuts at: 1419 2493 1419
Size: 1074 3899
BfrBI ATG'CAT
Cuts at: 1804 1804
Size: 4973
BglII A'GATC_T
Cuts at: 2488 4525 2488
Size: 2037 2936
BlpI GC'TnA_GC
Cuts at: 2085 2085
Size: 4973
Bme1580I G_kGCm'C
Cuts at: 2598 2691 3747 2598
Size: 93 1056 3824
Fragments arranged by size:
3824 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2185 2266 2601 4745 2185
Size: 81 335 2144 2413
Fragments arranged by size:
2413 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3340 3340
Size: 4973
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 747 1904 747
Size: 1157 3816
Bpu10I CC'TnA_GC
Cuts at: 833 833
Size: 4973
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 1014 2149 3028 3520 3818 304
Size: 710 1135 879 492 298 1459
Fragments arranged by size:
1459 1135 879 710 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4677 4677
Size: 4973
BsaAI yAC'GTr
Cuts at: 1638 2326 1638
Size: 688 4285
BsaBI GATnn'nnATC
Cuts at: 6 1553 2487 4524 6
Size: 1547 934 2037 455
Fragments arranged by size:
2037 1547 934 455
BsaHI Gr'CG_yC
Cuts at: 2404 2652 3354 4850 2404
Size: 248 702 1496 2527
Fragments arranged by size:
2527 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2162 2683 3361 3635 3782 4193 2162
Size: 521 678 274 147 411 2942
Fragments arranged by size:
2942 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1877 1883 1907 1913 1877
Size: 6 24 6 4937
Fragments arranged by size:
4937 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1475 1655 1475
Size: 180 4793
BseYI C'CCAG_C
Cuts at: 2398 3156 3733 4229 2398
Size: 758 577 496 3142
Fragments arranged by size:
3142 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2515 2561 3769 104
Size: 2411 46 1208 1308
Fragments arranged by size:
2411 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1987 2765 2955 3747 1987
Size: 778 190 792 3213
Fragments arranged by size:
3213 792 778 190
BsmI GAATG_Cn'
Cuts at: 1123 1123
Size: 4973
Bsp1286I G_dGCh'C
Cuts at: 1987 2598 2691 2765 2955 3017 3747 1987
Size: 611 93 74 190 62 730 3213
Fragments arranged by size:
3213 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4193 4193
Size: 4973
BspHI T'CATG_A
Cuts at: 4149 4149
Size: 4973
BspMI ACCTGCnnnn'nnnn_
Cuts at: 540 783 2023 2237 2539 2920 540
Size: 243 1240 214 302 381 2593
Fragments arranged by size:
2593 1240 381 302 243 214
BsrBI CCG'CTC
Cuts at: 50 1683 3265 3319 4803 4968 50
Size: 1633 1582 54 1484 165 55
Fragments arranged by size:
1633 1582 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1515 1611 1806 2885 1515
Size: 96 195 1079 3603
Fragments arranged by size:
3603 1079 195 96
BsrFI r'CCGG_y
Cuts at: 891 2971 3152 3361 891
Size: 2080 181 209 2503
Fragments arranged by size:
2503 2080 209 181
BssSI C'ACGA_G
Cuts at: 3244 3602 3244
Size: 358 4615
BstAPI GCAn_nnn'nTGC
Cuts at: 2350 2350
Size: 4973
BstBI TT'CG_AA
Cuts at: 3334 3334
Size: 4973
BstEII G'GTnAC_C
Cuts at: 1096 1096
Size: 4973
BstXI CCAn_nnnn'nTGG
Cuts at: 615 852 1953 615
Size: 237 1101 3635
Fragments arranged by size:
3635 1101 237
BstYI r'GATC_y
Cuts at: 1 196 1090 1861 1942 1998 2465 2488 2823
Size: 195 894 771 81 56 467 23 335
Cuts at: 2823 3069 4081 4167 4179 4525 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 894 771 467 449 346 335 246
195 86 81 56 23 12
Bsu36I CC'TnA_GG
Cuts at: 1338 1338
Size: 4973
BtgI C'CryG_G
Cuts at: 107 1455 107
Size: 1348 3625
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2923 3104 3267 2923
Size: 181 163 4629
Fragments arranged by size:
4629 181 163
BtsI GCAGTG_nn'
Cuts at: 1872 2216 1872
Size: 344 4629
DraI TTT'AAA
Cuts at: 1972 1972
Size: 4973
DrdI GACnn_nn'nnGTC
Cuts at: 2679 3537 2679
Size: 858 4115
EaeI y'GGCC_r
Cuts at: 2558 2732 3123 3150 4495 2558
Size: 174 391 27 1345 3036
Fragments arranged by size:
3036 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2558 2558
Size: 4973
EarI CTCTTCn'nnn_
Cuts at: 281 1590 2996 3206 281
Size: 1309 1406 210 2048
Fragments arranged by size:
2048 1406 1309 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 685 3181 3491 3637 4783 685
Size: 2496 310 146 1146 875
Fragments arranged by size:
2496 1146 875 310 146
EcoICRI GAG'CTC
Cuts at: 1985 1985
Size: 4973
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 431 719 747 1904 2798 3230 3977 4245
Size: 333 288 28 1157 894 432 747 268
Cuts at: 4245 98
Size: 826
Fragments arranged by size:
1157 894 826 747 432 333 288 268
28
EcoO109I rG'GnC_Cy
Cuts at: 2112 2112
Size: 4973
EcoRI G'AATT_C
Cuts at: 1977 1977
Size: 4973
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2992 2992
Size: 4973
FspI TGC'GCA
Cuts at: 2754 2754
Size: 4973
HaeII r_GCGC'y
Cuts at: 2407 2655 3677 4656 2407
Size: 248 1022 979 2724
Fragments arranged by size:
2724 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1251 1283 1399 1431 1533 1565 1736 1768 2815
Size: 32 116 32 102 32 171 32 1047
Cuts at: 2815 2845 2847 2877 1251
Size: 30 2 30 3347
Fragments arranged by size:
3347 1047 171 116 102 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 510 1959 2012 510
Size: 1449 53 3471
Fragments arranged by size:
3471 1449 53
HindIII A'AGCT_T
Cuts at: 251 751 1618 251
Size: 500 867 3606
Fragments arranged by size:
3606 867 500
KasI G'GCGC_C
Cuts at: 2403 2651 2403
Size: 248 4725
KpnI G_GTAC'C
Cuts at: 1993 1993
Size: 4973
MfeI C'AATT_G
Cuts at: 609 1358 609
Size: 749 4224
MluI A'CGCG_T
Cuts at: 4406 4406
Size: 4973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 833 1839 3644 3828 833
Size: 1006 1805 184 1978
Fragments arranged by size:
1978 1805 1006 184
MscI TGG'CCA
Cuts at: 2734 2734
Size: 4973
MslI CAynn'nnrTG
Cuts at: 1184 1493 2962 3089 1184
Size: 309 1469 127 3068
Fragments arranged by size:
3068 1469 309 127
MspA1I CmG'CkG
Cuts at: 2084 2398 2758 3771 4016 2084
Size: 314 360 1013 245 3041
Fragments arranged by size:
3041 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3154 3154
Size: 4973
NarI GG'CG_CC
Cuts at: 2404 2652 2404
Size: 248 4725
NcoI C'CATG_G
Cuts at: 107 107
Size: 4973
NgoMIV G'CCGG_C
Cuts at: 3152 3152
Size: 4973
NsiI A_TGCA'T
Cuts at: 1806 1806
Size: 4973
NspI r_CATG'y
Cuts at: 1183 2026 2526 3433 1183
Size: 843 500 907 2723
Fragments arranged by size:
2723 907 843 500
PciI A'CATG_T
Cuts at: 1179 3429 1179
Size: 2250 2723
PflMI CCAn_nnn'nTGG
Cuts at: 815 815
Size: 4973
PmeI GTTT'AAAC
Cuts at: 1972 1972
Size: 4973
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 184 216 4112 4144 184
Size: 32 3896 32 1013
Fragments arranged by size:
3896 1013 32 32
PstI C_TGCA'G
Cuts at: 551 1879 2020 2705 551
Size: 1328 141 685 2819
Fragments arranged by size:
2819 1328 685 141
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4973
PvuII CAG'CTG
Cuts at: 2398 2758 2398
Size: 360 4613
RsrII CG'GwC_CG
Cuts at: 3168 3168
Size: 4973
SacI G_AGCT'C
Cuts at: 1987 1987
Size: 4973
SalI G'TCGA_C
Cuts at: 2010 2010
Size: 4973
SapI GCTCTTCn'nnn_
Cuts at: 1590 2996 3206 1590
Size: 1406 210 3357
Fragments arranged by size:
3357 1406 210
SbfI CC_TGCA'GG
Cuts at: 2020 2020
Size: 4973
ScaI AGT'ACT
Cuts at: 4490 4490
Size: 4973
SfcI C'TryA_G
Cuts at: 33 547 1875 2016 2701 3694 3885 33
Size: 514 1328 141 685 993 191 1121
Fragments arranged by size:
1328 1121 993 685 514 191 141
SfoI GGC'GCC
Cuts at: 2405 2653 2405
Size: 248 4725
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4973
SmaI CCC'GGG
Cuts at: 1995 1995
Size: 4973
SmlI C'TyrA_G
Cuts at: 319 993 2128 3043 3535 3797 319
Size: 674 1135 915 492 262 1495
Fragments arranged by size:
1495 1135 915 674 492 262
SnaBI TAC'GTA
Cuts at: 1638 1638
Size: 4973
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4973
SphI G_CATG'C
Cuts at: 2026 2526 2026
Size: 500 4473
SspI AAT'ATT
Cuts at: 777 777
Size: 4973
StyI C'CwwG_G
Cuts at: 107 1042 1328 2107 2461 107
Size: 935 286 779 354 2619
Fragments arranged by size:
2619 935 779 354 286
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 898 1174 2556 2689 3148 3357 898
Size: 276 1382 133 459 209 2514
Fragments arranged by size:
2514 1382 459 276 209 133
TatI w'GTAC_w
Cuts at: 1429 4488 1429
Size: 3059 1914
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1883 1915 4490 4522 1883
Size: 32 2575 32 2334
Fragments arranged by size:
2575 2334 32 32
Tth111I GACn'n_nGTC
Cuts at: 514 2770 514
Size: 2256 2717
XbaI T'CTAG_A
Cuts at: 2004 2004
Size: 4973
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1743 2468 1743
Size: 725 4248
XmaI C'CCGG_G
Cuts at: 1993 1993
Size: 4973
XmnI GAAnn'nnTTC
Cuts at: 1521 1521
Size: 4973
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BglI BmgBI BmtI
BsgI BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI NdeI NheI
NotI NruI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI StuI SwaI XhoI ZraI