Restriction map for
pF1KA1386
(Circular) (Six-base) MAPSORT of: pF1KA1386.seq Check: 1600 from: 1 to: 6380
pF1KA1386 6380 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 810 2454 3418 810
Size: 1644 964 3772
Fragments arranged by size:
3772 1644 964
Acc65I G'GTAC_C
Cuts at: 3396 3396
Size: 6380
AclI AA'CG_TT
Cuts at: 6220 6220
Size: 6380
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 755 835 893 2036 2201 2620 4205 4637
Size: 342 80 58 1143 165 419 1585 432
Cuts at: 4637 5384 5652 413
Size: 747 268 1141
Fragments arranged by size:
1585 1143 1141 747 432 419 342 268
165 80 58
AflIII A'CryG_T
Cuts at: 1824 3732 4836 5813 1824
Size: 1908 1104 977 2391
Fragments arranged by size:
2391 1908 1104 977
AgeI A'CCGG_T
Cuts at: 4768 4768
Size: 6380
AleI CACnn'nnGTG
Cuts at: 2545 2545
Size: 6380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 578 1269 1726 578
Size: 691 457 5232
Fragments arranged by size:
5232 691 457
AlwNI CAG_nnn'CTG
Cuts at: 2476 2643 3685 5252 2476
Size: 167 1042 1567 3604
Fragments arranged by size:
3604 1567 1042 167
ApaLI G'TGCA_C
Cuts at: 337 5150 337
Size: 4813 1567
ApoI r'AATT_y
Cuts at: 127 1208 2141 2275 2401 3384 5733 5989 127
Size: 1081 933 134 126 983 2349 256 518
Fragments arranged by size:
2349 1081 983 933 518 256 134 126
AseI AT'TA_AT
Cuts at: 20 20
Size: 6380
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6380
AvaI C'yCGr_G
Cuts at: 727 2294 2825 3400 727
Size: 1567 531 575 3707
Fragments arranged by size:
3707 1567 575 531
BamHI G'GATC_C
Cuts at: 723 3405 723
Size: 2682 3698
BanI G'GyrC_C
Cuts at: 3396 3810 4058 4093 3396
Size: 414 248 35 5683
Fragments arranged by size:
5683 414 248 35
BanII G_rGCy'C
Cuts at: 236 402 891 1467 2004 2923 3394 4424 236
Size: 166 489 576 537 919 471 1030 2192
Fragments arranged by size:
2192 1030 919 576 537 489 471 166
BbeI G_GCGC'C
Cuts at: 3814 4062 3814
Size: 248 6132
BbsI GAAGACnn'nnnn_
Cuts at: 1145 2189 2666 1145
Size: 1044 477 4859
Fragments arranged by size:
4859 1044 477
BbvCI CC'TCA_GC
Cuts at: 191 191
Size: 6380
BciVI GTATCCnnnnn_n'
Cuts at: 4278 5039 6380 4278
Size: 761 1341 4278
Fragments arranged by size:
4278 1341 761
BclI T'GATC_A
Cuts at: 3900 3900
Size: 6380
BfrBI ATG'CAT
Cuts at: 357 357
Size: 6380
BglII A'GATC_T
Cuts at: 3895 5932 3895
Size: 2037 4343
BlpI GC'TnA_GC
Cuts at: 3492 3492
Size: 6380
Bme1580I G_kGCm'C
Cuts at: 341 4005 4098 5154 341
Size: 3664 93 1056 1567
Fragments arranged by size:
3664 1567 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 906 3592 3673 4008 6152 906
Size: 2686 81 335 2144 1134
Fragments arranged by size:
2686 2144 1134 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2018 2758 4747 2018
Size: 740 1989 3651
Fragments arranged by size:
3651 1989 740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1364 1805 2453 3059 1364
Size: 441 648 606 4685
Fragments arranged by size:
4685 648 606 441
Bpu10I CC'TnA_GC
Cuts at: 191 2917 191
Size: 2726 3654
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2071 3251 3327 3556 4435 4927 5225 2071
Size: 1180 76 229 879 492 298 3226
Fragments arranged by size:
3226 1180 879 492 298 229 76
BsaI GGTCTCn'nnnn_
Cuts at: 6084 6084
Size: 6380
BsaAI yAC'GTr
Cuts at: 1827 3733 1827
Size: 1906 4474
BsaBI GATnn'nnATC
Cuts at: 6 317 2379 3894 5931 6
Size: 311 2062 1515 2037 455
Fragments arranged by size:
2062 2037 1515 455 311
BsaHI Gr'CG_yC
Cuts at: 3811 4059 4761 6257 3811
Size: 248 702 1496 3934
Fragments arranged by size:
3934 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3569 4090 4768 5042 5189 5600 3569
Size: 521 678 274 147 411 4349
Fragments arranged by size:
4349 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 626 848 1846 2430 2453 3317 626
Size: 222 998 584 23 864 3689
Fragments arranged by size:
3689 998 864 584 222 23
BseYI C'CCAG_C
Cuts at: 1502 3104 3805 4563 5140 5636 1502
Size: 1602 701 758 577 496 2246
Fragments arranged by size:
2246 1602 758 701 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1140 2560 3116 1140
Size: 1420 556 4404
Fragments arranged by size:
4404 1420 556
BsiEI CG_ry'CG
Cuts at: 104 3922 3968 5176 104
Size: 3818 46 1208 1308
Fragments arranged by size:
3818 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 341 891 1467 2004 2066 2923 3394 4172 4362
Size: 550 576 537 62 857 471 778 190
Cuts at: 4362 5154 341
Size: 792 1567
Fragments arranged by size:
1567 857 792 778 576 550 537 471
190 62
BsmI GAATG_Cn'
Cuts at: 2081 2081
Size: 6380
Bsp1286I G_dGCh'C
Cuts at: 236 341 402 891 1467 2004 2066 2923 3394
Size: 105 61 489 576 537 62 857 471
Cuts at: 3394 4005 4098 4172 4362 4424 5154 236
Size: 611 93 74 190 62 730 1462
Fragments arranged by size:
1462 857 730 611 576 537 489 471
190 105 93 74 62 62 61
BspEI T'CCGG_A
Cuts at: 5600 5600
Size: 6380
BspHI T'CATG_A
Cuts at: 891 2873 3039 5556 891
Size: 1982 166 2517 1715
Fragments arranged by size:
2517 1982 1715 166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3430 3644 3946 4327 3430
Size: 214 302 381 5483
Fragments arranged by size:
5483 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4672 4726 6210 6375 50
Size: 4622 54 1484 165 55
Fragments arranged by size:
4622 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1453 4292 1453
Size: 2839 3541
BsrFI r'CCGG_y
Cuts at: 4378 4559 4768 4378
Size: 181 209 5990
Fragments arranged by size:
5990 209 181
BsrGI T'GTAC_A
Cuts at: 2256 2256
Size: 6380
BssSI C'ACGA_G
Cuts at: 4651 5009 4651
Size: 358 6022
BstAPI GCAn_nnn'nTGC
Cuts at: 3757 3757
Size: 6380
BstBI TT'CG_AA
Cuts at: 4741 4741
Size: 6380
BstYI r'GATC_y
Cuts at: 1 723 801 1432 3405 3872 3895 4230 4476
Size: 722 78 631 1973 467 23 335 246
Cuts at: 4476 5488 5574 5586 5932 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1973 1012 722 631 467 449 346 335
246 86 78 23 12
Bsu36I CC'TnA_GG
Cuts at: 570 797 2760 570
Size: 227 1963 4190
Fragments arranged by size:
4190 1963 227
BtgI C'CryG_G
Cuts at: 107 2546 107
Size: 2439 3941
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4330 4511 4674 4330
Size: 181 163 6036
Fragments arranged by size:
6036 181 163
BtsI GCAGTG_nn'
Cuts at: 1720 2695 3623 1720
Size: 975 928 4477
Fragments arranged by size:
4477 975 928
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2025 2060 2025
Size: 35 6345
DraI TTT'AAA
Cuts at: 1923 3379 1923
Size: 1456 4924
DraIII CAC_nnn'GTG
Cuts at: 988 988
Size: 6380
DrdI GACnn_nn'nnGTC
Cuts at: 2432 4086 4944 2432
Size: 1654 858 3868
Fragments arranged by size:
3868 1654 858
EaeI y'GGCC_r
Cuts at: 2985 3965 4139 4530 4557 5902 2985
Size: 980 174 391 27 1345 3463
Fragments arranged by size:
3463 1345 980 391 174 27
EagI C'GGCC_G
Cuts at: 3965 3965
Size: 6380
EarI CTCTTCn'nnn_
Cuts at: 438 1050 1501 1751 2260 2589 2639 4403 4613
Size: 612 451 250 509 329 50 1764 210
Cuts at: 4613 438
Size: 2205
Fragments arranged by size:
2205 1764 612 509 451 329 250 210
50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4588 4898 5044 6190 4588
Size: 310 146 1146 4778
Fragments arranged by size:
4778 1146 310 146
EcoICRI GAG'CTC
Cuts at: 889 1465 2002 2921 3392 889
Size: 576 537 919 471 3877
Fragments arranged by size:
3877 919 576 537 471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 755 835 893 1364 1805 2036 2201 2453
Size: 342 80 58 471 441 231 165 252
Cuts at: 2453 2620 3059 4205 4637 5384 5652 413
Size: 167 439 1146 432 747 268 1141
Fragments arranged by size:
1146 1141 747 471 441 439 432 342
268 252 231 167 165 80 58
EcoNI CCTnn'n_nnAGG
Cuts at: 1379 1379
Size: 6380
EcoO109I rG'GnC_Cy
Cuts at: 373 2991 3519 373
Size: 2618 528 3234
Fragments arranged by size:
3234 2618 528
EcoRI G'AATT_C
Cuts at: 2275 3384 2275
Size: 1109 5271
EcoRV GAT'ATC
Cuts at: 389 1278 389
Size: 889 5491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 223 418 841 898 1752 4399 223
Size: 195 423 57 854 2647 2204
Fragments arranged by size:
2647 2204 854 423 195 57
FspI TGC'GCA
Cuts at: 4161 4161
Size: 6380
HaeII r_GCGC'y
Cuts at: 3814 4062 5084 6063 3814
Size: 248 1022 979 4131
Fragments arranged by size:
4131 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1822 1854 2962 2994 4222 4252 4254 4284 1822
Size: 32 1108 32 1228 30 2 30 3918
Fragments arranged by size:
3918 1228 1108 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3419 3419
Size: 6380
HindIII A'AGCT_T
Cuts at: 875 920 875
Size: 45 6335
KasI G'GCGC_C
Cuts at: 3810 4058 3810
Size: 248 6132
KpnI G_GTAC'C
Cuts at: 3400 3400
Size: 6380
MfeI C'AATT_G
Cuts at: 1629 1990 1629
Size: 361 6019
MluI A'CGCG_T
Cuts at: 5813 5813
Size: 6380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 956 1284 5051 5235 956
Size: 328 3767 184 2101
Fragments arranged by size:
3767 2101 328 184
MscI TGG'CCA
Cuts at: 2987 4141 2987
Size: 1154 5226
MslI CAynn'nnrTG
Cuts at: 1114 2545 4369 4496 1114
Size: 1431 1824 127 2998
Fragments arranged by size:
2998 1824 1431 127
MspA1I CmG'CkG
Cuts at: 2983 3491 3805 4165 5178 5423 2983
Size: 508 314 360 1013 245 3940
Fragments arranged by size:
3940 1013 508 360 314 245
NaeI GCC'GGC
Cuts at: 4561 4561
Size: 6380
NarI GG'CG_CC
Cuts at: 3811 4059 3811
Size: 248 6132
NcoI C'CATG_G
Cuts at: 107 107
Size: 6380
NdeI CA'TA_TG
Cuts at: 780 1248 2103 780
Size: 468 855 5057
Fragments arranged by size:
5057 855 468
NgoMIV G'CCGG_C
Cuts at: 4559 4559
Size: 6380
NsiI A_TGCA'T
Cuts at: 359 359
Size: 6380
NspI r_CATG'y
Cuts at: 849 1713 2252 3433 3933 4840 849
Size: 864 539 1181 500 907 2389
Fragments arranged by size:
2389 1181 907 864 539 500
PciI A'CATG_T
Cuts at: 4836 4836
Size: 6380
PflMI CCAn_nnn'nTGG
Cuts at: 988 1367 988
Size: 379 6001
PmeI GTTT'AAAC
Cuts at: 3379 3379
Size: 6380
PmlI CAC'GTG
Cuts at: 1827 1827
Size: 6380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2429 2461 5519 5551 2429
Size: 32 3058 32 3258
Fragments arranged by size:
3258 3058 32 32
PpuMI rG'GwC_Cy
Cuts at: 373 2991 373
Size: 2618 3762
PstI C_TGCA'G
Cuts at: 461 843 2313 3427 4112 461
Size: 382 1470 1114 685 2729
Fragments arranged by size:
2729 1470 1114 685 382
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6380
PvuII CAG'CTG
Cuts at: 2983 3805 4165 2983
Size: 822 360 5198
Fragments arranged by size:
5198 822 360
RsrII CG'GwC_CG
Cuts at: 4575 4575
Size: 6380
SacI G_AGCT'C
Cuts at: 891 1467 2004 2923 3394 891
Size: 576 537 919 471 3877
Fragments arranged by size:
3877 919 576 537 471
SalI G'TCGA_C
Cuts at: 3417 3417
Size: 6380
SapI GCTCTTCn'nnn_
Cuts at: 438 1050 2589 4403 4613 438
Size: 612 1539 1814 210 2205
Fragments arranged by size:
2205 1814 1539 612 210
SbfI CC_TGCA'GG
Cuts at: 3427 3427
Size: 6380
ScaI AGT'ACT
Cuts at: 5897 5897
Size: 6380
SfcI C'TryA_G
Cuts at: 33 457 839 1321 2309 3083 3423 4108 5101
Size: 424 382 482 988 774 340 685 993
Cuts at: 5101 5292 33
Size: 191 1121
Fragments arranged by size:
1121 993 988 774 685 482 424 382
340 191
SfoI GGC'GCC
Cuts at: 3812 4060 3812
Size: 248 6132
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6380
SmaI CCC'GGG
Cuts at: 3402 3402
Size: 6380
SmlI C'TyrA_G
Cuts at: 2086 3266 3342 3535 4450 4942 5204 2086
Size: 1180 76 193 915 492 262 3262
Fragments arranged by size:
3262 1180 915 492 262 193 76
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6380
SphI G_CATG'C
Cuts at: 2252 3433 3933 2252
Size: 1181 500 4699
Fragments arranged by size:
4699 1181 500
SspI AAT'ATT
Cuts at: 290 2368 3243 290
Size: 2078 875 3427
Fragments arranged by size:
3427 2078 875
StuI AGG'CCT
Cuts at: 795 1339 2208 2535 795
Size: 544 869 327 4640
Fragments arranged by size:
4640 869 544 327
StyI C'CwwG_G
Cuts at: 107 1030 1512 1841 2329 2651 3514 3868 107
Size: 923 482 329 488 322 863 354 2619
Fragments arranged by size:
2619 923 863 488 482 354 329 322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3963 4096 4555 4764 3963
Size: 133 459 209 5579
Fragments arranged by size:
5579 459 209 133
TatI w'GTAC_w
Cuts at: 1801 2256 3363 5895 1801
Size: 455 1107 2532 2286
Fragments arranged by size:
2532 2286 1107 455
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1433 1465 2569 2601 5897 5929 1433
Size: 32 1104 32 3296 32 1884
Fragments arranged by size:
3296 1884 1104 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2429 2800 4177 2429
Size: 371 1377 4632
Fragments arranged by size:
4632 1377 371
XbaI T'CTAG_A
Cuts at: 3411 3411
Size: 6380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1980 3875 1980
Size: 1895 4485
XmaI C'CCGG_G
Cuts at: 3400 3400
Size: 6380
XmnI GAAnn'nnTTC
Cuts at: 1817 3242 1817
Size: 1425 4955
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I ClaI FseI FspAI
HpaI NheI NotI NruI PacI PasI PfoI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI