Restriction map for
pF1KA1395
(Circular) (Six-base) MAPSORT of: pF1KA1395.seq Check: 1223 from: 1 to: 9254
pF1KA1395 9254 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 168 2012 2951 3165 4688 5033 168
Size: 1844 939 214 1523 345 4389
Fragments arranged by size:
4389 1844 1523 939 345 214
AatII G_ACGT'C
Cuts at: 3957 5501 3957
Size: 1544 7710
AccI GT'mk_AC
Cuts at: 1928 5225 6292 1928
Size: 3297 1067 4890
Fragments arranged by size:
4890 3297 1067
Acc65I G'GTAC_C
Cuts at: 3730 4087 6270 3730
Size: 357 2183 6714
Fragments arranged by size:
6714 2183 357
AclI AA'CG_TT
Cuts at: 9094 9094
Size: 9254
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 1129 1770 3132 3503 4378 4650 5893 7079
Size: 857 641 1362 371 875 272 1243 1186
Cuts at: 7079 7511 7864 8526 272
Size: 432 353 662 1000
Fragments arranged by size:
1362 1243 1186 1000 875 857 662 641
432 371 353 272
AfeI AGC'GCT
Cuts at: 1329 3919 1329
Size: 2590 6664
AflIII A'CryG_T
Cuts at: 1763 6606 8406 8687 1763
Size: 4843 1800 281 2330
Fragments arranged by size:
4843 2330 1800 281
AgeI A'CCGG_T
Cuts at: 1920 1920
Size: 9254
AhdI GACnn_n'nnGTC
Cuts at: 594 3120 5100 594
Size: 2526 1980 4748
Fragments arranged by size:
4748 2526 1980
AleI CACnn'nnGTG
Cuts at: 1921 2245 2296 5390 5578 1921
Size: 324 51 3094 188 5597
Fragments arranged by size:
5597 3094 324 188 51
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3668 3668
Size: 9254
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1000 1032 4444 4476 4756 4788 5044 5076 5332
Size: 32 3412 32 280 32 256 32 256
Cuts at: 5332 5364 1000
Size: 32 4890
Fragments arranged by size:
4890 3412 280 256 256 32 32 32
32 32
AlwNI CAG_nnn'CTG
Cuts at: 221 307 495 1863 2137 2376 4767 6156 6171
Size: 86 188 1368 274 239 2391 1389 15
Cuts at: 6171 6559 7997 221
Size: 388 1438 1478
Fragments arranged by size:
2391 1478 1438 1389 1368 388 274 239
188 86 15
ApaI G_GGCC'C
Cuts at: 320 1406 2667 3165 3664 4434 5927 320
Size: 1086 1261 498 499 770 1493 3647
Fragments arranged by size:
3647 1493 1261 1086 770 499 498
ApaLI G'TGCA_C
Cuts at: 1303 2257 2299 3523 4969 5284 8092 1303
Size: 954 42 1224 1446 315 2808 2465
Fragments arranged by size:
2808 2465 1446 1224 954 315 42
ApoI r'AATT_y
Cuts at: 337 1897 2284 3235 6258 8607 8863 337
Size: 1560 387 951 3023 2349 256 728
Fragments arranged by size:
3023 2349 1560 951 728 387 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9254
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9254
AvaI C'yCGr_G
Cuts at: 185 313 374 397 633 1945 3788 4331 4865
Size: 128 61 23 236 1312 1843 543 534
Cuts at: 4865 5008 5707 6274 185
Size: 143 699 567 3165
Fragments arranged by size:
3165 1843 1312 699 567 543 534 236
143 128 61 23
BamHI G'GATC_C
Cuts at: 401 2081 6279 401
Size: 1680 4198 3376
Fragments arranged by size:
4198 3376 1680
BanI G'GyrC_C
Cuts at: 667 816 1045 1547 2102 3730 4087 5380 6186
Size: 149 229 502 555 1628 357 1293 806
Cuts at: 6186 6270 6684 6932 6967 667
Size: 84 414 248 35 2954
Fragments arranged by size:
2954 1628 1293 806 555 502 414 357
248 229 149 84 35
BanII G_rGCy'C
Cuts at: 266 314 320 398 640 935 1041 1406 1804
Size: 48 6 78 242 295 106 365 398
Cuts at: 1804 2240 2648 2667 3028 3165 3352 3443 3664
Size: 436 408 19 361 137 187 91 221
Cuts at: 3664 4101 4138 4434 4882 5204 5609 5917 5927
Size: 437 37 296 448 322 405 308 10
Cuts at: 5927 6268 7298 266
Size: 341 1030 2222
Fragments arranged by size:
2222 1030 448 437 436 408 405 398
365 361 341 322 308 296 295 242
221 187 137 106 91 78 48 37
19 10 6
BbeI G_GCGC'C
Cuts at: 1551 2106 5384 6688 6936 1551
Size: 555 3278 1304 248 3869
Fragments arranged by size:
3869 3278 1304 555 248
BbsI GAAGACnn'nnnn_
Cuts at: 653 3138 5848 5990 653
Size: 2485 2710 142 3917
Fragments arranged by size:
3917 2710 2485 142
BbvCI CC'TCA_GC
Cuts at: 3656 4715 3656
Size: 1059 8195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1269 1303 1711 1745 2995 3029 1269
Size: 34 408 34 1250 34 7494
Fragments arranged by size:
7494 1250 408 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3621 7152 8208 9254 3621
Size: 3531 1056 1046 3621
Fragments arranged by size:
3621 3531 1056 1046
BclI T'GATC_A
Cuts at: 6774 6774
Size: 9254
BglI GCCn_nnn'nGGC
Cuts at: 1297 1799 2636 2695 3097 4987 5685 1297
Size: 502 837 59 402 1890 698 4866
Fragments arranged by size:
4866 1890 837 698 502 402 59
BglII A'GATC_T
Cuts at: 980 5450 6769 8806 980
Size: 4470 1319 2037 1428
Fragments arranged by size:
4470 2037 1428 1319
BlpI GC'TnA_GC
Cuts at: 308 1499 2350 5734 6366 308
Size: 1191 851 3384 632 3196
Fragments arranged by size:
3384 3196 1191 851 632
Bme1580I G_kGCm'C
Cuts at: 320 1307 1406 2050 2261 2303 2667 3165 3527
Size: 987 99 644 211 42 364 498 362
Cuts at: 3527 3664 3840 4389 4398 4434 4973 5288 5927
Size: 137 176 549 9 36 539 315 639
Cuts at: 5927 6879 6972 8096 320
Size: 952 93 1124 1478
Fragments arranged by size:
1478 1124 987 952 644 639 549 539
498 364 362 315 211 176 137 99
93 42 36 9
BmgBI CAC'GTC
Cuts at: 2304 2304
Size: 9254
BmrI ACTGGGnnnn_n'
Cuts at: 474 2125 4953 6466 6547 6882 9026 474
Size: 1651 2828 1513 81 335 2144 702
Fragments arranged by size:
2828 2144 1651 1513 702 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7621 7621
Size: 9254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 605 1726 2077 2329 2584 2654 2875 3058
Size: 410 1121 351 252 255 70 221 183
Cuts at: 3058 3175 3290 3345 4315 5485 5968 6136 195
Size: 117 115 55 970 1170 483 168 3313
Fragments arranged by size:
3313 1170 1121 970 483 410 351 255
252 221 183 168 117 115 70 55
Bpu10I CC'TnA_GC
Cuts at: 1646 3656 4715 1646
Size: 2010 1059 6185
Fragments arranged by size:
6185 2010 1059
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 1579 2320 5593 6430 7309 8023 8321 895
Size: 684 741 3273 837 879 714 298 1828
Fragments arranged by size:
3273 1828 879 837 741 714 684 298
BsaI GGTCTCn'nnnn_
Cuts at: 8958 8958
Size: 9254
BsaAI yAC'GTr
Cuts at: 1764 2442 3270 5576 6607 1764
Size: 678 828 2306 1031 4411
Fragments arranged by size:
4411 2306 1031 828 678
BsaBI GATnn'nnATC
Cuts at: 6 4840 6768 8805 6
Size: 4834 1928 2037 455
Fragments arranged by size:
4834 2037 1928 455
BsaHI Gr'CG_yC
Cuts at: 1433 1548 2103 3954 5381 5498 5806 6685 6933
Size: 115 555 1851 1427 117 308 879 248
Cuts at: 6933 7635 9131 1433
Size: 702 1496 1556
Fragments arranged by size:
1851 1556 1496 1427 879 702 555 308
248 117 115
BsaWI w'CCGG_w
Cuts at: 1920 2555 4884 6443 6964 7642 8053 8200 8474
Size: 635 2329 1559 521 678 411 147 274
Cuts at: 8474 1920
Size: 2700
Fragments arranged by size:
2700 2329 1559 678 635 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1000 1030 4444 4474 5332 5362 5401 5431 1000
Size: 30 3414 30 858 30 39 30 4823
Fragments arranged by size:
4823 3414 858 39 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1972 2326 2851 4146 4480 4732 5089 5128 5482
Size: 354 525 1295 334 252 357 39 354
Cuts at: 5482 5806 1972
Size: 324 5420
Fragments arranged by size:
5420 1295 525 357 354 354 334 324
252 39
BseYI C'CCAG_C
Cuts at: 342 1330 1589 1852 2155 2201 2647 3436 3920
Size: 988 259 263 303 46 446 789 484
Cuts at: 3920 3944 3948 4935 5148 5336 6164 6176 6679
Size: 24 4 987 213 188 828 12 503
Cuts at: 6679 7437 8102 8510 342
Size: 758 665 408 1086
Fragments arranged by size:
1086 988 987 828 789 758 665 503
484 446 408 303 263 259 213 188
46 24 12 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 1242 1849 2701 2923 3273 4327 4765 5649
Size: 721 607 852 222 350 1054 438 884
Cuts at: 5649 521
Size: 4126
Fragments arranged by size:
4126 1054 884 852 721 607 438 350
222
BsiEI CG_ry'CG
Cuts at: 104 1247 1301 2044 2246 4979 6796 6842 8072
Size: 1143 54 743 202 2733 1817 46 1230
Cuts at: 8072 104
Size: 1286
Fragments arranged by size:
2733 1817 1286 1230 1143 743 202 54
46
BsiHKAI G_wGCw'C
Cuts at: 875 1307 2171 2240 2261 2303 3083 3266 3352
Size: 432 864 69 21 42 780 183 86
Cuts at: 3352 3527 4101 4679 4973 5288 5609 6268 7046
Size: 175 574 578 294 315 321 659 778
Cuts at: 7046 7236 8096 875
Size: 190 860 2033
Fragments arranged by size:
2033 864 860 780 778 659 578 574
432 321 315 294 190 183 175 86
69 42 21
BsiWI C'GTAC_G
Cuts at: 2874 2874
Size: 9254
BsmI GAATG_Cn'
Cuts at: 385 3490 385
Size: 3105 6149
BsmBI CGTCTCn'nnnn_
Cuts at: 581 5788 581
Size: 5207 4047
Bsp1286I G_dGCh'C
Cuts at: 266 314 320 398 640 875 935 1041 1307
Size: 48 6 78 242 235 60 106 266
Cuts at: 1307 1406 1804 2050 2171 2240 2261 2303 2648
Size: 99 398 246 121 69 21 42 345
Cuts at: 2648 2667 3028 3083 3165 3266 3352 3443 3527
Size: 19 361 55 82 101 86 91 84
Cuts at: 3527 3664 3840 4101 4138 4389 4398 4434 4679
Size: 137 176 261 37 251 9 36 245
Cuts at: 4679 4882 4973 5204 5288 5609 5917 5927 6268
Size: 203 91 231 84 321 308 10 341
Cuts at: 6268 6879 6972 7046 7236 7298 8096 266
Size: 611 93 74 190 62 798 1424
Fragments arranged by size:
1424 798 611 398 361 345 341 321
308 266 261 251 246 245 242 235
231 203 190 176 137 121 106 101
99 93 91 91 86 84 84 82
78 74 69 62 60 55 48 42
37 36 21 19 10 9 6
BspHI T'CATG_A
Cuts at: 7686 7686
Size: 9254
BspMI ACCTGCnnnn'nnnn_
Cuts at: 168 1763 2012 2951 3165 3611 4574 4611 4688
Size: 1595 249 939 214 446 963 37 77
Cuts at: 4688 4746 4787 5033 6304 6518 6820 7201 168
Size: 58 41 246 1271 214 302 381 2221
Fragments arranged by size:
2221 1595 1271 963 939 446 381 302
249 246 214 214 77 58 41 37
BsrBI CCG'CTC
Cuts at: 50 732 1076 7546 7600 9084 9249 50
Size: 682 344 6470 54 1484 165 55
Fragments arranged by size:
6470 1484 682 344 165 55 54
BsrDI GCAATG_nn'
Cuts at: 709 768 2534 3722 3758 7166 709
Size: 59 1766 1188 36 3408 2797
Fragments arranged by size:
3408 2797 1766 1188 59 36
BsrFI r'CCGG_y
Cuts at: 293 755 785 1370 1685 1866 1920 2113 3903
Size: 462 30 585 315 181 54 193 1790
Cuts at: 3903 5179 5459 7252 7433 293
Size: 1276 280 1793 181 2114
Fragments arranged by size:
2114 1793 1790 1276 585 462 315 280
193 181 181 54 30
BsrGI T'GTAC_A
Cuts at: 4370 4853 5417 4370
Size: 483 564 8207
Fragments arranged by size:
8207 564 483
BssHII G'CGCG_C
Cuts at: 5688 5688
Size: 9254
BssSI C'ACGA_G
Cuts at: 2455 2833 3259 3584 4234 4984 7525 8233 2455
Size: 378 426 325 650 750 2541 708 3476
Fragments arranged by size:
3476 2541 750 708 650 426 378 325
BstAPI GCAn_nnn'nTGC
Cuts at: 221 2376 2946 3251 3531 3835 6128 6156 6631
Size: 2155 570 305 280 304 2293 28 475
Cuts at: 6631 221
Size: 2844
Fragments arranged by size:
2844 2293 2155 570 475 305 304 280
28
BstBI TT'CG_AA
Cuts at: 3398 7615 3398
Size: 4217 5037
BstEII G'GTnAC_C
Cuts at: 3384 4623 5392 5619 3384
Size: 1239 769 227 7019
Fragments arranged by size:
7019 1239 769 227
BstYI r'GATC_y
Cuts at: 1 401 887 944 980 2081 5450 5975 6279
Size: 400 486 57 36 1101 3369 525 304
Cuts at: 6279 6746 6769 7104 7350 7656 7668 7754 8806
Size: 467 23 335 246 306 12 86 1052
Cuts at: 8806 1
Size: 449
Fragments arranged by size:
3369 1101 1052 525 486 467 449 400
335 306 304 246 86 57 36 23
12
Bsu36I CC'TnA_GG
Cuts at: 322 605 688 2107 3476 4498 6205 322
Size: 283 83 1419 1369 1022 1707 3371
Fragments arranged by size:
3371 1707 1419 1369 1022 283 83
BtgI C'CryG_G
Cuts at: 107 389 440 836 2066 2511 2583 2700 2774
Size: 282 51 396 1230 445 72 117 74
Cuts at: 2774 3637 3752 5030 5192 5279 107
Size: 863 115 1278 162 87 4082
Fragments arranged by size:
4082 1278 1230 863 445 396 282 162
117 115 87 74 72 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7204 7385 7548 7204
Size: 181 163 8910
Fragments arranged by size:
8910 181 163
BtsI GCAGTG_nn'
Cuts at: 201 669 1899 2048 2086 2395 2589 2857 2874
Size: 468 1230 149 38 309 194 268 17
Cuts at: 2874 3011 3607 4358 4389 6497 201
Size: 137 596 751 31 2108 2958
Fragments arranged by size:
2958 2108 1230 751 596 468 309 268
194 149 137 38 31 17
ClaI AT'CG_AT
Cuts at: 5431 5431
Size: 9254
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4259 4294 4259
Size: 35 9219
DraI TTT'AAA
Cuts at: 6253 6253
Size: 9254
DraIII CAC_nnn'GTG
Cuts at: 2442 3270 2442
Size: 828 8426
DrdI GACnn_nn'nnGTC
Cuts at: 6960 8304 6960
Size: 1344 7910
EaeI y'GGCC_r
Cuts at: 158 295 1244 1298 1310 1372 2041 2243 2405
Size: 137 949 54 12 62 669 202 162
Cuts at: 2405 2490 2816 2952 3074 3179 3540 3590 3749
Size: 85 326 136 122 105 361 50 159
Cuts at: 3749 3755 4418 4593 4916 4976 5276 5819 6839
Size: 6 663 175 323 60 300 543 1020
Cuts at: 6839 7013 7404 7431 8776 158
Size: 174 391 27 1345 636
Fragments arranged by size:
1345 1020 949 669 663 636 543 391
361 326 323 300 202 175 174 162
159 137 136 122 105 85 62 60
54 50 27 12 6
EagI C'GGCC_G
Cuts at: 1244 1298 2041 2243 4976 6839 1244
Size: 54 743 202 2733 1863 3659
Fragments arranged by size:
3659 2733 1863 743 202 54
EarI CTCTTCn'nnn_
Cuts at: 1528 2920 4165 4405 4667 6001 7277 7487 1528
Size: 1392 1245 240 262 1334 1276 210 3295
Fragments arranged by size:
3295 1392 1334 1276 1245 262 240 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4182 5070 7462 8204 8350 9064 4182
Size: 888 2392 742 146 714 4372
Fragments arranged by size:
4372 2392 888 742 714 146
EcoICRI GAG'CTC
Cuts at: 2238 3350 4099 5607 6266 2238
Size: 1112 749 1508 659 5226
Fragments arranged by size:
5226 1508 1112 749 659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 272 605 1129 1726 1770 2077 2329 2584
Size: 77 333 524 597 44 307 252 255
Cuts at: 2584 2654 2875 3058 3132 3175 3290 3345 3503
Size: 70 221 183 74 43 115 55 158
Cuts at: 3503 4315 4378 4650 5485 5893 5968 6136 7079
Size: 812 63 272 835 408 75 168 943
Cuts at: 7079 7511 7864 8526 195
Size: 432 353 662 923
Fragments arranged by size:
943 923 835 812 662 597 524 432
408 353 333 307 272 255 252 221
183 168 158 115 77 75 74 70
63 55 44 43
EcoNI CCTnn'n_nnAGG
Cuts at: 2311 5008 6139 2311
Size: 2697 1131 5426
Fragments arranged by size:
5426 2697 1131
EcoO109I rG'GnC_Cy
Cuts at: 240 317 327 549 1402 1403 1518 1848 2268
Size: 77 10 222 853 1 115 330 420
Cuts at: 2268 2474 2663 2664 3660 4430 4431 4764 4827
Size: 206 189 1 996 770 1 333 63
Cuts at: 4827 5874 5923 5941 6075 6393 240
Size: 1047 49 18 134 318 3101
Fragments arranged by size:
3101 1047 996 853 770 420 333 330
318 222 206 189 134 115 77 63
49 18 10 1 1 1
EcoRI G'AATT_C
Cuts at: 337 3235 6258 337
Size: 2898 3023 3333
Fragments arranged by size:
3333 3023 2898
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3000 7273 3000
Size: 4273 4981
FseI GG_CCGG'CC
Cuts at: 1689 1689
Size: 9254
FspI TGC'GCA
Cuts at: 1810 3229 7035 1810
Size: 1419 3806 4029
Fragments arranged by size:
4029 3806 1419
HaeII r_GCGC'y
Cuts at: 126 222 1331 1371 1551 2106 2188 2938 3910
Size: 96 1109 40 180 555 82 750 972
Cuts at: 3910 3921 4556 5384 6171 6688 6936 8166 8937
Size: 11 635 828 787 517 248 1230 771
Cuts at: 8937 126
Size: 443
Fragments arranged by size:
1230 1109 972 828 787 771 750 635
555 517 443 248 180 96 82 40
11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 580 612 872 904 936 968 994 1026 1333
Size: 32 260 32 32 32 26 32 307
Cuts at: 1333 1339 1365 1371 3106 3138 4676 4708 5086
Size: 6 26 6 1735 32 1538 32 378
Cuts at: 5086 5118 7096 7126 7128 7158 580
Size: 32 1978 30 2 30 2676
Fragments arranged by size:
2676 1978 1735 1538 378 307 260 32
32 32 32 32 32 32 32 30
30 26 26 6 6 2
HincII GTy'rAC
Cuts at: 6293 6293
Size: 9254
HindIII A'AGCT_T
Cuts at: 5542 5542
Size: 9254
KasI G'GCGC_C
Cuts at: 1547 2102 5380 6684 6932 1547
Size: 555 3278 1304 248 3869
Fragments arranged by size:
3869 3278 1304 555 248
KpnI G_GTAC'C
Cuts at: 3734 4091 6274 3734
Size: 357 2183 6714
Fragments arranged by size:
6714 2183 357
MluI A'CGCG_T
Cuts at: 8687 8687
Size: 9254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1112 3941 5087 5114 8013 8197 1112
Size: 2829 1146 27 2899 184 2169
Fragments arranged by size:
2899 2829 2169 1146 184 27
MscI TGG'CCA
Cuts at: 1312 2407 2492 2818 2954 3181 3542 3751 3757
Size: 1095 85 326 136 227 361 209 6
Cuts at: 3757 4420 5821 7015 1312
Size: 663 1401 1194 3551
Fragments arranged by size:
3551 1401 1194 1095 663 361 326 227
209 136 85 6
MslI CAynn'nnrTG
Cuts at: 1921 2245 2296 3062 5390 5578 5776 7243 7370
Size: 324 51 766 2328 188 198 1467 127
Cuts at: 7370 1921
Size: 3805
Fragments arranged by size:
3805 2328 1467 766 324 198 188 127
51
MspA1I CmG'CkG
Cuts at: 346 442 534 703 1338 1889 2030 2585 2601
Size: 96 92 169 635 551 141 555 16
Cuts at: 2601 3672 3948 5281 6180 6365 6679 7039 7823
Size: 1071 276 1333 899 185 314 360 784
Cuts at: 7823 8068 346
Size: 245 1532
Fragments arranged by size:
1532 1333 1071 899 784 635 555 551
360 314 276 245 185 169 141 96
92 16
NaeI GCC'GGC
Cuts at: 295 1372 1687 3905 5181 7435 295
Size: 1077 315 2218 1276 2254 2114
Fragments arranged by size:
2254 2218 2114 1276 1077 315
NarI GG'CG_CC
Cuts at: 1548 2103 5381 6685 6933 1548
Size: 555 3278 1304 248 3869
Fragments arranged by size:
3869 3278 1304 555 248
NcoI C'CATG_G
Cuts at: 107 3752 5192 107
Size: 3645 1440 4169
Fragments arranged by size:
4169 3645 1440
NgoMIV G'CCGG_C
Cuts at: 293 1370 1685 3903 5179 7433 293
Size: 1077 315 2218 1276 2254 2114
Fragments arranged by size:
2254 2218 2114 1276 1077 315
NotI GC'GGCC_GC
Cuts at: 4976 4976
Size: 9254
NspI r_CATG'y
Cuts at: 1284 2199 3396 5835 6307 6807 8410 1284
Size: 915 1197 2439 472 500 1603 2128
Fragments arranged by size:
2439 2128 1603 1197 915 500 472
PasI CC'CwG_GG
Cuts at: 234 1679 3804 234
Size: 1445 2125 5684
Fragments arranged by size:
5684 2125 1445
PciI A'CATG_T
Cuts at: 8406 8406
Size: 9254
PflMI CCAn_nnn'nTGG
Cuts at: 2445 2919 4965 6183 2445
Size: 474 2046 1218 5516
Fragments arranged by size:
5516 2046 1218 474
PfoI T'CCnGG_A
Cuts at: 2306 2654 2999 4448 4760 5128 5246 5978 2306
Size: 348 345 1449 312 368 118 732 5582
Fragments arranged by size:
5582 1449 732 368 348 345 312 118
PmeI GTTT'AAAC
Cuts at: 6253 6253
Size: 9254
PmlI CAC'GTG
Cuts at: 2442 2442
Size: 9254
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1000 1032 4444 4476 5332 5364 7700 7732 1000
Size: 32 3412 32 856 32 2336 32 2522
Fragments arranged by size:
3412 2522 2336 856 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 240 327 1518 1848 2268 4764 4827 5874 5941
Size: 87 1191 330 420 2496 63 1047 67
Cuts at: 5941 240
Size: 3553
Fragments arranged by size:
3553 2496 1191 1047 420 330 87 67
63
PspOMI G'GGCC_C
Cuts at: 316 1402 2663 3161 3660 4430 5923 316
Size: 1086 1261 498 499 770 1493 3647
Fragments arranged by size:
3647 1493 1261 1086 770 499 498
PspXI vC'TCGA_Gb
Cuts at: 5008 5008
Size: 9254
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1744 1776 1744
Size: 32 9222
PstI C_TGCA'G
Cuts at: 226 371 1441 1894 2096 2852 3917 5300 6152
Size: 145 1070 453 202 756 1065 1383 852
Cuts at: 6152 6301 6986 226
Size: 149 685 2494
Fragments arranged by size:
2494 1383 1070 1065 852 756 685 453
202 149 145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9254
PvuII CAG'CTG
Cuts at: 346 703 1338 1889 2030 2601 3948 6180 6679
Size: 357 635 551 141 571 1347 2232 499
Cuts at: 6679 7039 346
Size: 360 2561
Fragments arranged by size:
2561 2232 1347 635 571 551 499 360
357 141
RsrII CG'GwC_CG
Cuts at: 7449 7449
Size: 9254
SacI G_AGCT'C
Cuts at: 2240 3352 4101 5609 6268 2240
Size: 1112 749 1508 659 5226
Fragments arranged by size:
5226 1508 1112 749 659
SacII CC_GC'GG
Cuts at: 443 2586 5282 443
Size: 2143 2696 4415
Fragments arranged by size:
4415 2696 2143
SalI G'TCGA_C
Cuts at: 6291 6291
Size: 9254
SanDI GG'GwC_CC
Cuts at: 240 5941 240
Size: 5701 3553
SapI GCTCTTCn'nnn_
Cuts at: 4165 4667 6001 7277 7487 4165
Size: 502 1334 1276 210 5932
Fragments arranged by size:
5932 1334 1276 502 210
SbfI CC_TGCA'GG
Cuts at: 6301 6301
Size: 9254
ScaI AGT'ACT
Cuts at: 8771 8771
Size: 9254
SexAI A'CCwGG_T
Cuts at: 329 4900 329
Size: 4571 4683
SfcI C'TryA_G
Cuts at: 33 222 367 846 1437 1746 1890 2092 2848
Size: 189 145 479 591 309 144 202 756
Cuts at: 2848 3913 5296 5896 5918 6148 6297 6982 7950
Size: 1065 1383 600 22 230 149 685 968
Cuts at: 7950 8141 33
Size: 191 1146
Fragments arranged by size:
1383 1146 1065 968 756 685 600 591
479 309 230 202 191 189 149 145
144 22
SfoI GGC'GCC
Cuts at: 1549 2104 5382 6686 6934 1549
Size: 555 3278 1304 248 3869
Fragments arranged by size:
3869 3278 1304 555 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9254
SgrAI Cr'CCGG_yG
Cuts at: 1920 1920
Size: 9254
SmaI CCC'GGG
Cuts at: 187 315 376 399 3790 4333 4867 6276 187
Size: 128 61 23 3391 543 534 1409 3165
Fragments arranged by size:
3391 3165 1409 543 534 128 61 23
SmlI C'TyrA_G
Cuts at: 910 1594 2335 5008 5608 6409 7324 8038 8300
Size: 684 741 2673 600 801 915 714 262
Cuts at: 8300 910
Size: 1864
Fragments arranged by size:
2673 1864 915 801 741 714 684 600
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9254
SphI G_CATG'C
Cuts at: 1284 2199 6307 6807 1284
Size: 915 4108 500 3731
Fragments arranged by size:
4108 3731 915 500
SrfI GCCC'GGGC
Cuts at: 315 315
Size: 9254
StuI AGG'CCT
Cuts at: 1912 4012 4240 5305 5560 1912
Size: 2100 228 1065 255 5606
Fragments arranged by size:
5606 2100 1065 255 228
StyI C'CwwG_G
Cuts at: 107 407 1694 3017 3380 3752 4217 4241 5192
Size: 300 1287 1323 363 372 465 24 951
Cuts at: 5192 6388 6742 107
Size: 1196 354 2619
Fragments arranged by size:
2619 1323 1287 1196 951 465 372 363
354 300 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1063 2193 2844 3873 4438 6204 6837 6970 7429
Size: 1130 651 1029 565 1766 633 133 459
Cuts at: 7429 7638 1063
Size: 209 2679
Fragments arranged by size:
2679 1766 1130 1029 651 633 565 459
209 133
TatI w'GTAC_w
Cuts at: 282 1832 3995 4370 4853 5417 8769 282
Size: 1550 2163 375 483 564 3352 767
Fragments arranged by size:
3352 2163 1550 767 564 483 375
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5314 5346 8771 8803 5314
Size: 32 3425 32 5765
Fragments arranged by size:
5765 3425 32 32
Tth111I GACn'n_nGTC
Cuts at: 466 1574 5497 7051 466
Size: 1108 3923 1554 2669
Fragments arranged by size:
3923 2669 1554 1108
XbaI T'CTAG_A
Cuts at: 6285 6285
Size: 9254
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1065 1701 4224 6749 1065
Size: 636 2523 2525 3570
Fragments arranged by size:
3570 2525 2523 636
XhoI C'TCGA_G
Cuts at: 5008 5008
Size: 9254
XmaI C'CCGG_G
Cuts at: 185 313 374 397 3788 4331 4865 6274 185
Size: 128 61 23 3391 543 534 1409 3165
Fragments arranged by size:
3391 3165 1409 543 534 128 61 23
XmnI GAAnn'nnTTC
Cuts at: 770 4079 4112 5137 770
Size: 3309 33 1025 4887
Fragments arranged by size:
4887 3309 1025 33
ZraI GAC'GTC
Cuts at: 3955 5499 3955
Size: 1544 7710
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BfrBI BmtI BspEI BstXI
BstZ17I EcoRV FspAI HpaI MfeI NdeI NheI NruI
NsiI PacI PshAI PsiI SfiI SnaBI SspI SwaI