Restriction map for
pF1KA1398
(Circular) (Six-base) MAPSORT of: pF1KA1398.seq Check: 6903 from: 1 to: 7793
pF1KA1398 7793 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4008 4008
Size: 7793
AatII G_ACGT'C
Cuts at: 3482 3482
Size: 7793
AccI GT'mk_AC
Cuts at: 193 4831 193
Size: 4638 3155
Acc65I G'GTAC_C
Cuts at: 4809 4809
Size: 7793
AclI AA'CG_TT
Cuts at: 7633 7633
Size: 7793
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 764 2890 3868 4447 4562 4624 4699 5618 6050
Size: 2126 978 579 115 62 75 919 432
Cuts at: 6050 6403 7065 764
Size: 353 662 1492
Fragments arranged by size:
2126 1492 978 919 662 579 432 353
115 75 62
AflIII A'CryG_T
Cuts at: 4148 5145 6945 7226 4148
Size: 997 1800 281 4715
Fragments arranged by size:
4715 1800 997 281
AleI CACnn'nnGTG
Cuts at: 2827 2827
Size: 7793
AlwNI CAG_nnn'CTG
Cuts at: 688 2368 2883 3088 3156 5098 6536 688
Size: 1680 515 205 68 1942 1438 1945
Fragments arranged by size:
1945 1942 1680 1438 515 205 68
ApaI G_GGCC'C
Cuts at: 903 963 1023 1053 1083 1113 1143 1173 1203
Size: 60 60 30 30 30 30 30 30
Cuts at: 1203 1293 1353 1383 1413 1443 1473 1503 1533
Size: 90 60 30 30 30 30 30 30
Cuts at: 1533 1563 1593 1713 1743 1803 1833 1863 1893
Size: 30 30 120 30 60 30 30 30
Cuts at: 1893 1923 1983 2013 2474 4377 903
Size: 30 60 30 461 1903 4319
Fragments arranged by size:
4319 1903 461 120 90 60 60 60
60 60 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30
ApaLI G'TGCA_C
Cuts at: 6631 6631
Size: 7793
ApoI r'AATT_y
Cuts at: 579 4797 7146 7402 579
Size: 4218 2349 256 970
Fragments arranged by size:
4218 2349 970 256
AscI GG'CGCG_CC
Cuts at: 4650 4650
Size: 7793
AseI AT'TA_AT
Cuts at: 20 20
Size: 7793
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7793
AvaI C'yCGr_G
Cuts at: 2807 4067 4813 2807
Size: 1260 746 5787
Fragments arranged by size:
5787 1260 746
BamHI G'GATC_C
Cuts at: 4818 4818
Size: 7793
BanI G'GyrC_C
Cuts at: 636 651 664 1318 2409 4039 4395 4777 4809
Size: 15 13 654 1091 1630 356 382 32
Cuts at: 4809 5223 5471 5506 636
Size: 414 248 35 2923
Fragments arranged by size:
2923 1630 1091 654 414 382 356 248
35 32 15 13
BanII G_rGCy'C
Cuts at: 414 753 903 963 993 1023 1053 1083 1113
Size: 339 150 60 30 30 30 30 30
Cuts at: 1113 1143 1173 1203 1233 1263 1293 1353 1383
Size: 30 30 30 30 30 30 60 30
Cuts at: 1383 1413 1443 1473 1503 1533 1563 1593 1713
Size: 30 30 30 30 30 30 30 120
Cuts at: 1713 1743 1803 1833 1863 1893 1923 1983 2013
Size: 30 60 30 30 30 30 60 30
Cuts at: 2013 2043 2073 2103 2133 2187 2404 2474 2735
Size: 30 30 30 30 54 217 70 261
Cuts at: 2735 3050 3575 3875 4058 4377 4807 5837 414
Size: 315 525 300 183 319 430 1030 2370
Fragments arranged by size:
2370 1030 525 430 339 319 315 300
261 217 183 150 120 70 60 60
60 60 54 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
BbeI G_GCGC'C
Cuts at: 4043 4399 5227 5475 4043
Size: 356 828 248 6361
Fragments arranged by size:
6361 828 356 248
BbsI GAAGACnn'nnnn_
Cuts at: 4435 4568 4687 4435
Size: 133 119 7541
Fragments arranged by size:
7541 133 119
BbvCI CC'TCA_GC
Cuts at: 1223 1313 2003 4234 1223
Size: 90 690 2231 4782
Fragments arranged by size:
4782 2231 690 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3499 3533 4291 4325 4767 4801 3499
Size: 34 758 34 442 34 6491
Fragments arranged by size:
6491 758 442 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5691 6747 7793 5691
Size: 1056 1046 5691
Fragments arranged by size:
5691 1056 1046
BclI T'GATC_A
Cuts at: 360 5313 360
Size: 4953 2840
BglI GCCn_nnn'nGGC
Cuts at: 988 1108 1138 1168 1198 1258 1348 1378 1438
Size: 120 30 30 30 60 90 30 60
Cuts at: 1438 1468 1498 1528 1558 1588 1708 1738 1798
Size: 30 30 30 30 30 120 30 60
Cuts at: 1798 1828 1858 1888 1918 2038 2779 3277 3493
Size: 30 30 30 30 120 741 498 216
Cuts at: 3493 3643 3664 3760 988
Size: 150 21 96 5021
Fragments arranged by size:
5021 741 498 216 150 120 120 120
96 90 60 60 60 30 30 30
30 30 30 30 30 30 30 30
30 30 30 21
BglII A'GATC_T
Cuts at: 5308 7345 5308
Size: 2037 5756
BlpI GC'TnA_GC
Cuts at: 3049 4905 3049
Size: 1856 5937
Bme1580I G_kGCm'C
Cuts at: 656 903 963 1023 1053 1083 1113 1143 1173
Size: 247 60 60 30 30 30 30 30
Cuts at: 1173 1203 1293 1323 1353 1383 1413 1443 1473
Size: 30 90 30 30 30 30 30 30
Cuts at: 1473 1503 1533 1563 1593 1713 1743 1803 1833
Size: 30 30 30 30 120 30 60 30
Cuts at: 1833 1863 1893 1923 1983 2013 2230 2474 4377
Size: 30 30 30 60 30 217 244 1903
Cuts at: 4377 4780 5418 5511 6635 656
Size: 403 638 93 1124 1814
Fragments arranged by size:
1903 1814 1124 638 403 247 244 217
120 93 90 60 60 60 60 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30
BmrI ACTGGGnnnn_n'
Cuts at: 433 652 2273 5005 5086 5421 7565 433
Size: 219 1621 2732 81 335 2144 661
Fragments arranged by size:
2732 2144 1621 661 335 219 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3894 4611 4797 6160 3894
Size: 717 186 1363 5527
Fragments arranged by size:
5527 1363 717 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2938 3370 3469 3553 3574 4042 4459 4480 2938
Size: 432 99 84 21 468 417 21 6251
Fragments arranged by size:
6251 468 432 417 99 84 21 21
Bpu10I CC'TnA_GC
Cuts at: 1223 1313 2003 2132 3574 4234 1223
Size: 90 690 129 1442 660 4782
Fragments arranged by size:
4782 1442 690 660 129 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3508 4969 5848 6562 6860 3508
Size: 1461 879 714 298 4441
Fragments arranged by size:
4441 1461 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2523 3980 7497 2523
Size: 1457 3517 2819
Fragments arranged by size:
3517 2819 1457
BsaAI yAC'GTr
Cuts at: 2865 4350 5146 2865
Size: 1485 796 5512
Fragments arranged by size:
5512 1485 796
BsaBI GATnn'nnATC
Cuts at: 6 365 5307 7344 6
Size: 359 4942 2037 455
Fragments arranged by size:
4942 2037 455 359
BsaHI Gr'CG_yC
Cuts at: 3169 3479 4040 4396 5224 5472 6174 7670 3169
Size: 310 561 356 828 248 702 1496 3292
Fragments arranged by size:
3292 1496 828 702 561 356 310 248
BsaWI w'CCGG_w
Cuts at: 2897 4982 5503 6181 6592 6739 7013 2897
Size: 2085 521 678 411 147 274 3677
Fragments arranged by size:
3677 2085 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3520 3550 3874 3904 4006 4036 4135 4165 3520
Size: 30 324 30 102 30 99 30 7148
Fragments arranged by size:
7148 324 102 99 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 498 3202 3373 3867 3925 4021 4024 4027 4063
Size: 2704 171 494 58 96 3 3 36
Cuts at: 4063 4066 4519 4603 498
Size: 3 453 84 3688
Fragments arranged by size:
3688 2704 494 453 171 96 84 58
36 3 3 3
BseYI C'CCAG_C
Cuts at: 2558 3410 4369 4377 4401 5218 5976 6641 7049
Size: 852 959 8 24 817 758 665 408
Cuts at: 7049 2558
Size: 3302
Fragments arranged by size:
3302 959 852 817 758 665 408 24
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 2851 2896 3295 3667 463
Size: 2388 45 399 372 4589
Fragments arranged by size:
4589 2388 399 372 45
BsiEI CG_ry'CG
Cuts at: 104 3659 4190 4496 5335 5381 6611 104
Size: 3555 531 306 839 46 1230 1286
Fragments arranged by size:
3555 1286 1230 839 531 306 46
BsiHKAI G_wGCw'C
Cuts at: 1953 2187 2735 2864 3050 3575 3925 4058 4148
Size: 234 548 129 186 525 350 133 90
Cuts at: 4148 4807 5585 5775 6635 1953
Size: 659 778 190 860 3111
Fragments arranged by size:
3111 860 778 659 548 525 350 234
190 186 133 129 90
BsmBI CGTCTCn'nnnn_
Cuts at: 3907 3961 3907
Size: 54 7739
Bsp1286I G_dGCh'C
Cuts at: 414 656 753 903 963 993 1023 1053 1083
Size: 242 97 150 60 30 30 30 30
Cuts at: 1083 1113 1143 1173 1203 1233 1263 1293 1323
Size: 30 30 30 30 30 30 30 30
Cuts at: 1323 1353 1383 1413 1443 1473 1503 1533 1563
Size: 30 30 30 30 30 30 30 30
Cuts at: 1563 1593 1713 1743 1803 1833 1863 1893 1923
Size: 30 120 30 60 30 30 30 30
Cuts at: 1923 1953 1983 2013 2043 2073 2103 2133 2187
Size: 30 30 30 30 30 30 30 54
Cuts at: 2187 2230 2404 2474 2735 2864 3050 3575 3875
Size: 43 174 70 261 129 186 525 300
Cuts at: 3875 3925 4058 4148 4377 4780 4807 5418 5511
Size: 50 133 90 229 403 27 611 93
Cuts at: 5511 5585 5775 5837 6635 414
Size: 74 190 62 798 1572
Fragments arranged by size:
1572 798 611 525 403 300 261 242
229 190 186 174 150 133 129 120
97 93 90 74 70 62 60 60
54 50 43 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 27
BspEI T'CCGG_A
Cuts at: 2897 2897
Size: 7793
BspHI T'CATG_A
Cuts at: 363 6225 363
Size: 5862 1931
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3141 3998 4008 4843 5057 5359 5740 3141
Size: 857 10 835 214 302 381 5194
Fragments arranged by size:
5194 857 835 381 302 214 10
BsrBI CCG'CTC
Cuts at: 50 6085 6139 7623 7788 50
Size: 6035 54 1484 165 55
Fragments arranged by size:
6035 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5705 5705
Size: 7793
BsrFI r'CCGG_y
Cuts at: 4561 5791 5972 4561
Size: 1230 181 6382
Fragments arranged by size:
6382 1230 181
BssHII G'CGCG_C
Cuts at: 4650 4650
Size: 7793
BssSI C'ACGA_G
Cuts at: 2994 4659 6064 6772 2994
Size: 1665 1405 708 4015
Fragments arranged by size:
4015 1665 1405 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2883 3156 3353 4152 5170 2883
Size: 273 197 799 1018 5506
Fragments arranged by size:
5506 1018 799 273 197
BstBI TT'CG_AA
Cuts at: 601 6154 601
Size: 5553 2240
BstEII G'GTnAC_C
Cuts at: 2632 2632
Size: 7793
BstXI CCAn_nnnn'nTGG
Cuts at: 4224 4224
Size: 7793
BstYI r'GATC_y
Cuts at: 1 2573 2894 3819 4818 5285 5308 5643 5889
Size: 2572 321 925 999 467 23 335 246
Cuts at: 5889 6195 6207 6293 7345 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2572 1052 999 925 467 449 335 321
306 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 2522 3375 3638 3653 3746 3866 107
Size: 2415 853 263 15 93 120 4034
Fragments arranged by size:
4034 2415 853 263 120 93 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4316 5743 5924 6087 4316
Size: 1427 181 163 6022
Fragments arranged by size:
6022 1427 181 163
BtsI GCAGTG_nn'
Cuts at: 3925 5036 3925
Size: 1111 6682
DraI TTT'AAA
Cuts at: 4792 4792
Size: 7793
DraIII CAC_nnn'GTG
Cuts at: 3809 3809
Size: 7793
DrdI GACnn_nn'nnGTC
Cuts at: 5499 6843 5499
Size: 1344 6449
EaeI y'GGCC_r
Cuts at: 2684 3029 3302 3650 3656 4187 4493 5378 5552
Size: 345 273 348 6 531 306 885 174
Cuts at: 5552 5943 5970 7315 2684
Size: 391 27 1345 3162
Fragments arranged by size:
3162 1345 885 531 391 348 345 306
273 174 27 6
EagI C'GGCC_G
Cuts at: 3656 4187 4493 5378 3656
Size: 531 306 885 6071
Fragments arranged by size:
6071 885 531 306
EarI CTCTTCn'nnn_
Cuts at: 329 2660 2909 3991 4085 4336 5816 6026 329
Size: 2331 249 1082 94 251 1480 210 2096
Fragments arranged by size:
2331 2096 1480 1082 251 249 210 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 727 907 3280 3352 3415 3511 3979 6001 6743
Size: 180 2373 72 63 96 468 2022 742
Cuts at: 6743 6889 7603 727
Size: 146 714 917
Fragments arranged by size:
2373 2022 917 742 714 468 180 146
96 72 63
EcoICRI GAG'CTC
Cuts at: 2185 2733 3048 3573 4056 4805 2185
Size: 548 315 525 483 749 5173
Fragments arranged by size:
5173 749 548 525 483 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 764 2890 2938 3370 3469 3553 3574 3868 4042
Size: 2126 48 432 99 84 21 294 174
Cuts at: 4042 4447 4459 4480 4562 4624 4699 5618 6050
Size: 405 12 21 82 62 75 919 432
Cuts at: 6050 6403 7065 764
Size: 353 662 1492
Fragments arranged by size:
2126 1492 919 662 432 432 405 353
294 174 99 84 82 75 62 48
21 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2953 3211 3448 3694 2953
Size: 258 237 246 7052
Fragments arranged by size:
7052 258 246 237
EcoO109I rG'GnC_Cy
Cuts at: 899 959 960 1019 1049 1079 1109 1139 1169
Size: 60 1 59 30 30 30 30 30
Cuts at: 1169 1199 1289 1349 1379 1409 1439 1469 1499
Size: 30 90 60 30 30 30 30 30
Cuts at: 1499 1529 1559 1560 1589 1590 1619 1649 1679
Size: 30 30 1 29 1 29 30 30
Cuts at: 1679 1709 1739 1769 1799 1829 1859 1889 1919
Size: 30 30 30 30 30 30 30 30
Cuts at: 1919 1979 2009 2273 2303 2471 3153 3192 3441
Size: 60 30 264 30 168 682 39 249
Cuts at: 3441 3753 3835 4373 4374 4413 4932 899
Size: 312 82 538 1 39 519 3760
Fragments arranged by size:
3760 682 538 519 312 264 249 168
90 82 60 60 60 59 39 39
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 29 29 1 1 1 1
EcoRI G'AATT_C
Cuts at: 579 4797 579
Size: 4218 3575
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4135 5812 4135
Size: 1677 6116
FspI TGC'GCA
Cuts at: 3346 5574 3346
Size: 2228 5565
HaeII r_GCGC'y
Cuts at: 3207 4043 4199 4399 5227 5475 6705 7476 3207
Size: 836 156 200 828 248 1230 771 3524
Fragments arranged by size:
3524 1230 836 828 771 248 200 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 345 377 711 743 2174 2206 2563 2595 2886
Size: 32 334 32 1431 32 357 32 291
Cuts at: 2886 2918 3520 3552 3874 3906 5635 5665 5667
Size: 32 602 32 322 32 1729 30 2
Cuts at: 5667 5697 345
Size: 30 2441
Fragments arranged by size:
2441 1729 1431 602 357 334 322 291
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 194 4634 4832 194
Size: 4440 198 3155
Fragments arranged by size:
4440 3155 198
HindIII A'AGCT_T
Cuts at: 3034 3034
Size: 7793
HpaI GTT'AAC
Cuts at: 4634 4634
Size: 7793
KasI G'GCGC_C
Cuts at: 4039 4395 5223 5471 4039
Size: 356 828 248 6361
Fragments arranged by size:
6361 828 356 248
KpnI G_GTAC'C
Cuts at: 4813 4813
Size: 7793
MluI A'CGCG_T
Cuts at: 7226 7226
Size: 7793
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 457 6552 6736 457
Size: 6095 184 1514
Fragments arranged by size:
6095 1514 184
MscI TGG'CCA
Cuts at: 2686 3031 3652 5554 2686
Size: 345 621 1902 4925
Fragments arranged by size:
4925 1902 621 345
MslI CAynn'nnrTG
Cuts at: 2827 5782 5909 2827
Size: 2955 127 4711
Fragments arranged by size:
4711 2955 127
MspA1I CmG'CkG
Cuts at: 562 2335 2562 2661 2799 2883 2916 2940 3111
Size: 1773 227 99 138 84 33 24 171
Cuts at: 3111 3136 3162 3868 4425 4437 4479 4602 4698
Size: 25 26 706 557 12 42 123 96
Cuts at: 4698 4740 4904 5218 5578 6362 6607 562
Size: 42 164 314 360 784 245 1748
Fragments arranged by size:
1773 1748 784 706 557 360 314 245
227 171 164 138 123 99 96 84
42 42 33 26 25 24 12
NaeI GCC'GGC
Cuts at: 4563 5974 4563
Size: 1411 6382
NarI GG'CG_CC
Cuts at: 4040 4396 5224 5472 4040
Size: 356 828 248 6361
Fragments arranged by size:
6361 828 356 248
NcoI C'CATG_G
Cuts at: 107 3638 3746 107
Size: 3531 108 4154
Fragments arranged by size:
4154 3531 108
NdeI CA'TA_TG
Cuts at: 219 219
Size: 7793
NgoMIV G'CCGG_C
Cuts at: 4561 5972 4561
Size: 1411 6382
NotI GC'GGCC_GC
Cuts at: 4187 4187
Size: 7793
NspI r_CATG'y
Cuts at: 2844 3981 4526 4846 5346 6949 2844
Size: 1137 545 320 500 1603 3688
Fragments arranged by size:
3688 1603 1137 545 500 320
PasI CC'CwG_GG
Cuts at: 969 969
Size: 7793
PciI A'CATG_T
Cuts at: 6945 6945
Size: 7793
PflMI CCAn_nnn'nTGG
Cuts at: 688 688
Size: 7793
PfoI T'CCnGG_A
Cuts at: 3386 3617 4457 3386
Size: 231 840 6722
Fragments arranged by size:
6722 840 231
PmeI GTTT'AAAC
Cuts at: 4792 4792
Size: 7793
PmlI CAC'GTG
Cuts at: 2865 2865
Size: 7793
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3414 3446 6239 6271 3414
Size: 32 2793 32 4936
Fragments arranged by size:
4936 2793 32 32
PpuMI rG'GwC_Cy
Cuts at: 1619 1649 1679 1769 2273 2303 3153 4413 1619
Size: 30 30 90 504 30 850 1260 4999
Fragments arranged by size:
4999 1260 850 504 90 30 30 30
PshAI GACnn'nnGTC
Cuts at: 2515 2515
Size: 7793
PspOMI G'GGCC_C
Cuts at: 899 959 1019 1049 1079 1109 1139 1169 1199
Size: 60 60 30 30 30 30 30 30
Cuts at: 1199 1289 1349 1379 1409 1439 1469 1499 1529
Size: 90 60 30 30 30 30 30 30
Cuts at: 1529 1559 1589 1709 1739 1799 1829 1859 1889
Size: 30 30 120 30 60 30 30 30
Cuts at: 1889 1919 1979 2009 2470 4373 899
Size: 30 60 30 461 1903 4319
Fragments arranged by size:
4319 1903 461 120 90 60 60 60
60 60 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30
PstI C_TGCA'G
Cuts at: 2888 2954 3161 3167 3200 3818 3932 3995 4670
Size: 66 207 6 33 618 114 63 675
Cuts at: 4670 4733 4840 5525 2888
Size: 63 107 685 5156
Fragments arranged by size:
5156 685 675 618 207 114 107 66
63 63 33 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7793
PvuII CAG'CTG
Cuts at: 562 2335 2661 2799 2883 2916 2940 3136 3162
Size: 1773 326 138 84 33 24 196 26
Cuts at: 3162 4425 4437 4602 4698 4740 5218 5578 562
Size: 1263 12 165 96 42 478 360 2777
Fragments arranged by size:
2777 1773 1263 478 360 326 196 165
138 96 84 42 33 26 24 12
RsrII CG'GwC_CG
Cuts at: 5988 5988
Size: 7793
SacI G_AGCT'C
Cuts at: 2187 2735 3050 3575 4058 4807 2187
Size: 548 315 525 483 749 5173
Fragments arranged by size:
5173 749 548 525 483 315
SacII CC_GC'GG
Cuts at: 3869 3869
Size: 7793
SalI G'TCGA_C
Cuts at: 192 4830 192
Size: 4638 3155
SanDI GG'GwC_CC
Cuts at: 1619 1649 1679 1769 2273 2303 1619
Size: 30 30 90 504 30 7109
Fragments arranged by size:
7109 504 90 30 30 30
SapI GCTCTTCn'nnn_
Cuts at: 3991 4336 5816 6026 3991
Size: 345 1480 210 5758
Fragments arranged by size:
5758 1480 345 210
SbfI CC_TGCA'GG
Cuts at: 4840 4840
Size: 7793
ScaI AGT'ACT
Cuts at: 7310 7310
Size: 7793
SexAI A'CCwGG_T
Cuts at: 4133 4133
Size: 7793
SfcI C'TryA_G
Cuts at: 33 572 2884 2950 3157 3163 3196 3814 3928
Size: 539 2312 66 207 6 33 618 114
Cuts at: 3928 3991 4666 4729 4836 5521 6489 6680 33
Size: 63 675 63 107 685 968 191 1146
Fragments arranged by size:
2312 1146 968 685 675 618 539 207
191 114 107 66 63 63 33 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 1108 1138 1168 1198 1348 1378 1438 1468 1498
Size: 30 30 30 150 30 60 30 30
Cuts at: 1498 1528 1558 1588 1708 1738 1798 1828 1858
Size: 30 30 30 120 30 60 30 30
Cuts at: 1858 1888 1918 3664 1108
Size: 30 30 1746 5237
Fragments arranged by size:
5237 1746 150 120 60 60 30 30
30 30 30 30 30 30 30 30
30 30 30 30
SfoI GGC'GCC
Cuts at: 4041 4397 5225 5473 4041
Size: 356 828 248 6361
Fragments arranged by size:
6361 828 356 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7793
SmaI CCC'GGG
Cuts at: 2809 4069 4815 2809
Size: 1260 746 5787
Fragments arranged by size:
5787 1260 746
SmlI C'TyrA_G
Cuts at: 3523 4948 5863 6577 6839 3523
Size: 1425 915 714 262 4477
Fragments arranged by size:
4477 1425 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7793
SphI G_CATG'C
Cuts at: 2844 3981 4846 5346 2844
Size: 1137 865 500 5291
Fragments arranged by size:
5291 1137 865 500
StuI AGG'CCT
Cuts at: 3667 4594 3667
Size: 927 6866
StyI C'CwwG_G
Cuts at: 107 131 515 629 668 819 849 879 2163
Size: 24 384 114 39 151 30 30 1284
Cuts at: 2163 2313 3128 3638 3701 3746 4031 4217 4927
Size: 150 815 510 63 45 285 186 710
Cuts at: 4927 5281 107
Size: 354 2619
Fragments arranged by size:
2619 1284 815 710 510 384 354 285
186 151 150 114 63 45 39 30
30 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 652 5376 5509 5968 6177 652
Size: 4724 133 459 209 2268
Fragments arranged by size:
4724 2268 459 209 133
TatI w'GTAC_w
Cuts at: 7308 7308
Size: 7793
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4006 4038 7310 7342 4006
Size: 32 3272 32 4457
Fragments arranged by size:
4457 3272 32 32
Tth111I GACn'n_nGTC
Cuts at: 2358 3214 5590 2358
Size: 856 2376 4561
Fragments arranged by size:
4561 2376 856
XbaI T'CTAG_A
Cuts at: 4824 4824
Size: 7793
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2937 3000 3645 4354 5288 2937
Size: 63 645 709 934 5442
Fragments arranged by size:
5442 934 709 645 63
XmaI C'CCGG_G
Cuts at: 2807 4067 4813 2807
Size: 1260 746 5787
Fragments arranged by size:
5787 1260 746
XmnI GAAnn'nnTTC
Cuts at: 3429 3429
Size: 7793
ZraI GAC'GTC
Cuts at: 3480 3480
Size: 7793
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AlfI AloI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmI BsrGI BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI MfeI NheI NruI
NsiI PacI PsiI PspXI PsrI SgrAI SnaBI SrfI
SspI SwaI XhoI