Restriction map for
pF1KA1401
(Circular) (Six-base) MAPSORT of: pF1KA1401.seq Check: 1104 from: 1 to: 5525
pF1KA1401 5525 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 260 1158 2563 260
Size: 898 1405 3222
Fragments arranged by size:
3222 1405 898
Acc65I G'GTAC_C
Cuts at: 2344 2541 2344
Size: 197 5328
AclI AA'CG_TT
Cuts at: 5365 5365
Size: 5525
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1809 1817 3350 3782 4135 4797 718
Size: 1091 8 1533 432 353 662 1446
Fragments arranged by size:
1533 1446 1091 662 432 353 8
AfeI AGC'GCT
Cuts at: 748 748
Size: 5525
AflII C'TTAA_G
Cuts at: 1624 1624
Size: 5525
AflIII A'CryG_T
Cuts at: 2252 2877 4677 4958 2252
Size: 625 1800 281 2819
Fragments arranged by size:
2819 1800 625 281
AgeI A'CCGG_T
Cuts at: 613 613
Size: 5525
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2146 2146
Size: 5525
AlwNI CAG_nnn'CTG
Cuts at: 363 954 1153 2830 4268 363
Size: 591 199 1677 1438 1620
Fragments arranged by size:
1677 1620 1438 591 199
ApaLI G'TGCA_C
Cuts at: 4363 4363
Size: 5525
ApoI r'AATT_y
Cuts at: 386 926 1598 2222 2529 4878 5134 386
Size: 540 672 624 307 2349 256 777
Fragments arranged by size:
2349 777 672 624 540 307 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5525
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5525
AvaI C'yCGr_G
Cuts at: 2545 2545
Size: 5525
BamHI G'GATC_C
Cuts at: 1650 2550 1650
Size: 900 4625
BanI G'GyrC_C
Cuts at: 369 916 2344 2541 2955 3203 3238 369
Size: 547 1428 197 414 248 35 2656
Fragments arranged by size:
2656 1428 547 414 248 197 35
BanII G_rGCy'C
Cuts at: 1949 2539 3569 1949
Size: 590 1030 3905
Fragments arranged by size:
3905 1030 590
BbeI G_GCGC'C
Cuts at: 2959 3207 2959
Size: 248 5277
BbsI GAAGACnn'nnnn_
Cuts at: 2418 2418
Size: 5525
BbvCI CC'TCA_GC
Cuts at: 516 1508 1763 516
Size: 992 255 4278
Fragments arranged by size:
4278 992 255
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 157 191 157
Size: 34 5491
BciVI GTATCCnnnnn_n'
Cuts at: 3423 4479 5525 3423
Size: 1056 1046 3423
Fragments arranged by size:
3423 1056 1046
BclI T'GATC_A
Cuts at: 3045 3045
Size: 5525
BglI GCCn_nnn'nGGC
Cuts at: 112 2059 112
Size: 1947 3578
BglII A'GATC_T
Cuts at: 3040 5077 3040
Size: 2037 3488
BlpI GC'TnA_GC
Cuts at: 1234 2637 1234
Size: 1403 4122
Bme1580I G_kGCm'C
Cuts at: 374 487 919 3150 3243 4367 374
Size: 113 432 2231 93 1124 1532
Fragments arranged by size:
2231 1532 1124 432 113 93
BmrI ACTGGGnnnn_n'
Cuts at: 2737 2818 3153 5297 2737
Size: 81 335 2144 2965
Fragments arranged by size:
2965 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3892 3892
Size: 5525
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2337 2337
Size: 5525
Bpu10I CC'TnA_GC
Cuts at: 226 516 644 805 1508 1763 226
Size: 290 128 161 703 255 3988
Fragments arranged by size:
3988 703 290 255 161 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 1655 2375 2489 2701 3580 4294 4592 118
Size: 1537 720 114 212 879 714 298 1051
Fragments arranged by size:
1537 1051 879 720 714 298 212 114
BsaI GGTCTCn'nnnn_
Cuts at: 1019 5229 1019
Size: 4210 1315
BsaAI yAC'GTr
Cuts at: 2878 2878
Size: 5525
BsaBI GATnn'nnATC
Cuts at: 6 3039 5076 6
Size: 3033 2037 455
Fragments arranged by size:
3033 2037 455
BsaHI Gr'CG_yC
Cuts at: 2956 3204 3906 5402 2956
Size: 248 702 1496 3079
Fragments arranged by size:
3079 1496 702 248
BsaWI w'CCGG_w
Cuts at: 613 1634 2714 3235 3913 4324 4471 4745 613
Size: 1021 1080 521 678 411 147 274 1393
Fragments arranged by size:
1393 1080 1021 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1751 1781 2073 2103 1751
Size: 30 292 30 5173
Fragments arranged by size:
5173 292 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 341 757 1240 1243 1414 341
Size: 416 483 3 171 4452
Fragments arranged by size:
4452 483 416 171 3
BseYI C'CCAG_C
Cuts at: 330 602 2950 3708 4373 4781 330
Size: 272 2348 758 665 408 1074
Fragments arranged by size:
2348 1074 758 665 408 272
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 361 520 170
Size: 191 159 5175
Fragments arranged by size:
5175 191 159
BsiEI CG_ry'CG
Cuts at: 104 3067 3113 4343 104
Size: 2963 46 1230 1286
Fragments arranged by size:
2963 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1949 2539 3317 3507 4367 1949
Size: 590 778 190 860 3107
Fragments arranged by size:
3107 860 778 590 190
BsmI GAATG_Cn'
Cuts at: 2135 2135
Size: 5525
BsmBI CGTCTCn'nnnn_
Cuts at: 1812 1812
Size: 5525
Bsp1286I G_dGCh'C
Cuts at: 374 487 919 1949 2539 3150 3243 3317 3507
Size: 113 432 1030 590 611 93 74 190
Cuts at: 3507 3569 4367 374
Size: 62 798 1532
Fragments arranged by size:
1532 1030 798 611 590 432 190 113
93 74 62
BspEI T'CCGG_A
Cuts at: 1634 1634
Size: 5525
BspHI T'CATG_A
Cuts at: 1866 3957 1866
Size: 2091 3434
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2575 2789 3091 3472 2575
Size: 214 302 381 4628
Fragments arranged by size:
4628 381 302 214
BsrBI CCG'CTC
Cuts at: 50 132 3817 3871 5355 5520 50
Size: 82 3685 54 1484 165 55
Fragments arranged by size:
3685 1484 165 82 55 54
BsrDI GCAATG_nn'
Cuts at: 1088 2130 2141 3437 1088
Size: 1042 11 1296 3176
Fragments arranged by size:
3176 1296 1042 11
BsrFI r'CCGG_y
Cuts at: 613 3523 3704 613
Size: 2910 181 2434
Fragments arranged by size:
2910 2434 181
BssSI C'ACGA_G
Cuts at: 3796 4504 3796
Size: 708 4817
BstAPI GCAn_nnn'nTGC
Cuts at: 2902 2902
Size: 5525
BstBI TT'CG_AA
Cuts at: 3886 3886
Size: 5525
BstXI CCAn_nnnn'nTGG
Cuts at: 1345 2471 1345
Size: 1126 4399
BstYI r'GATC_y
Cuts at: 1 181 531 1191 1650 2169 2550 3017 3040
Size: 180 350 660 459 519 381 467 23
Cuts at: 3040 3375 3621 3927 3939 4025 5077 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 660 519 467 459 449 381 350
335 306 246 180 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1655 1655
Size: 5525
BtgI C'CryG_G
Cuts at: 107 1645 2349 107
Size: 1538 704 3283
Fragments arranged by size:
3283 1538 704
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 256 3475 3656 3819 256
Size: 3219 181 163 1962
Fragments arranged by size:
3219 1962 181 163
BtsI GCAGTG_nn'
Cuts at: 743 2003 2768 743
Size: 1260 765 3500
Fragments arranged by size:
3500 1260 765
DraI TTT'AAA
Cuts at: 1040 1731 2524 1040
Size: 691 793 4041
Fragments arranged by size:
4041 793 691
DraIII CAC_nnn'GTG
Cuts at: 543 543
Size: 5525
DrdI GACnn_nn'nnGTC
Cuts at: 2182 3231 4575 2182
Size: 1049 1344 3132
Fragments arranged by size:
3132 1344 1049
EaeI y'GGCC_r
Cuts at: 2352 3110 3284 3675 3702 5047 2352
Size: 758 174 391 27 1345 2830
Fragments arranged by size:
2830 1345 758 391 174 27
EagI C'GGCC_G
Cuts at: 3110 3110
Size: 5525
EarI CTCTTCn'nnn_
Cuts at: 316 893 1424 1544 1937 3548 3758 316
Size: 577 531 120 393 1611 210 2083
Fragments arranged by size:
2083 1611 577 531 393 210 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 176 3733 4475 4621 5335 176
Size: 3557 742 146 714 366
Fragments arranged by size:
3557 742 714 366 146
EcoICRI GAG'CTC
Cuts at: 1947 2537 1947
Size: 590 4935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1809 1817 2337 3350 3782 4135 4797 718
Size: 1091 8 520 1013 432 353 662 1446
Fragments arranged by size:
1446 1091 1013 662 520 432 353 8
EcoNI CCTnn'n_nnAGG
Cuts at: 397 519 1141 397
Size: 122 622 4781
Fragments arranged by size:
4781 622 122
EcoO109I rG'GnC_Cy
Cuts at: 648 1068 1284 2664 648
Size: 420 216 1380 3509
Fragments arranged by size:
3509 1380 420 216
EcoRI G'AATT_C
Cuts at: 1598 2529 1598
Size: 931 4594
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1144 1269 3544 1144
Size: 125 2275 3125
Fragments arranged by size:
3125 2275 125
FspI TGC'GCA
Cuts at: 3306 3306
Size: 5525
HaeII r_GCGC'y
Cuts at: 750 1974 2959 3207 4437 5208 750
Size: 1224 985 248 1230 771 1067
Fragments arranged by size:
1230 1224 1067 985 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1390 1422 2196 2228 2312 2344 2430 2462 3367
Size: 32 774 32 84 32 86 32 905
Cuts at: 3367 3397 3399 3429 1390
Size: 30 2 30 3486
Fragments arranged by size:
3486 905 774 86 84 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 261 2564 261
Size: 2303 3222
KasI G'GCGC_C
Cuts at: 2955 3203 2955
Size: 248 5277
KpnI G_GTAC'C
Cuts at: 2348 2545 2348
Size: 197 5328
MluI A'CGCG_T
Cuts at: 4958 4958
Size: 5525
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 679 963 1502 4284 4468 679
Size: 284 539 2782 184 1736
Fragments arranged by size:
2782 1736 539 284 184
MscI TGG'CCA
Cuts at: 2354 3286 2354
Size: 932 4593
MslI CAynn'nnrTG
Cuts at: 446 1081 1644 3514 3641 446
Size: 635 563 1870 127 2330
Fragments arranged by size:
2330 1870 635 563 127
MspA1I CmG'CkG
Cuts at: 192 330 411 2011 2636 2950 3310 4094 4339
Size: 138 81 1600 625 314 360 784 245
Cuts at: 4339 192
Size: 1378
Fragments arranged by size:
1600 1378 784 625 360 314 245 138
81
NaeI GCC'GGC
Cuts at: 3706 3706
Size: 5525
NarI GG'CG_CC
Cuts at: 2956 3204 2956
Size: 248 5277
NcoI C'CATG_G
Cuts at: 107 1645 2349 107
Size: 1538 704 3283
Fragments arranged by size:
3283 1538 704
NdeI CA'TA_TG
Cuts at: 2451 2451
Size: 5525
NgoMIV G'CCGG_C
Cuts at: 3704 3704
Size: 5525
NspI r_CATG'y
Cuts at: 2256 2578 3078 4681 2256
Size: 322 500 1603 3100
Fragments arranged by size:
3100 1603 500 322
PciI A'CATG_T
Cuts at: 2252 4677 2252
Size: 2425 3100
PmeI GTTT'AAAC
Cuts at: 2524 2524
Size: 5525
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 3971
Size: 32 5493
PpuMI rG'GwC_Cy
Cuts at: 1068 1284 1068
Size: 216 5309
PstI C_TGCA'G
Cuts at: 2010 2572 3257 2010
Size: 562 685 4278
Fragments arranged by size:
4278 685 562
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5525
PvuII CAG'CTG
Cuts at: 330 411 2950 3310 330
Size: 81 2539 360 2545
Fragments arranged by size:
2545 2539 360 81
RsrII CG'GwC_CG
Cuts at: 3720 3720
Size: 5525
SacI G_AGCT'C
Cuts at: 1949 2539 1949
Size: 590 4935
SalI G'TCGA_C
Cuts at: 259 2562 259
Size: 2303 3222
SapI GCTCTTCn'nnn_
Cuts at: 1937 3548 3758 1937
Size: 1611 210 3704
Fragments arranged by size:
3704 1611 210
SbfI CC_TGCA'GG
Cuts at: 2572 2572
Size: 5525
ScaI AGT'ACT
Cuts at: 2402 5042 2402
Size: 2640 2885
SfcI C'TryA_G
Cuts at: 33 1106 2006 2147 2568 3253 4221 4412 33
Size: 1073 900 141 421 685 968 191 1146
Fragments arranged by size:
1146 1073 968 900 685 421 191 141
SfoI GGC'GCC
Cuts at: 2957 3205 2957
Size: 248 5277
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5525
SgrAI Cr'CCGG_yG
Cuts at: 613 613
Size: 5525
SmaI CCC'GGG
Cuts at: 2547 2547
Size: 5525
SmlI C'TyrA_G
Cuts at: 133 1624 1670 2390 2504 2680 3595 4309 4571
Size: 1491 46 720 114 176 915 714 262
Cuts at: 4571 133
Size: 1087
Fragments arranged by size:
1491 1087 915 720 714 262 176 114
46
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5525
SphI G_CATG'C
Cuts at: 2578 3078 2578
Size: 500 5025
SspI AAT'ATT
Cuts at: 1688 1688
Size: 5525
StuI AGG'CCT
Cuts at: 1622 1622
Size: 5525
StyI C'CwwG_G
Cuts at: 107 1645 1937 2349 2659 3013 107
Size: 1538 292 412 310 354 2619
Fragments arranged by size:
2619 1538 412 354 310 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 322 478 3108 3241 3700 3909 322
Size: 156 2630 133 459 209 1938
Fragments arranged by size:
2630 1938 459 209 156 133
TatI w'GTAC_w
Cuts at: 438 2400 5040 438
Size: 1962 2640 923
Fragments arranged by size:
2640 1962 923
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 656 688 2073 2105 2263 2295 5042 5074 656
Size: 32 1385 32 158 32 2747 32 1107
Fragments arranged by size:
2747 1385 1107 158 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3322 3322
Size: 5525
XbaI T'CTAG_A
Cuts at: 2556 2556
Size: 5525
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 599 682 3020 599
Size: 83 2338 3104
Fragments arranged by size:
3104 2338 83
XmaI C'CCGG_G
Cuts at: 2545 2545
Size: 5525
XmnI GAAnn'nnTTC
Cuts at: 1664 1664
Size: 5525
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AloI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SwaI XhoI ZraI