Restriction map for
pF1KA1402
(Circular) (Six-base) MAPSORT of: pF1KA1402.seq Check: 7105 from: 1 to: 5429
pF1KA1402 5429 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 537 2158 537
Size: 1621 3808
AccI GT'mk_AC
Cuts at: 1691 2467 1691
Size: 776 4653
Acc65I G'GTAC_C
Cuts at: 1039 1772 2445 1039
Size: 733 673 4023
Fragments arranged by size:
4023 733 673
AclI AA'CG_TT
Cuts at: 5269 5269
Size: 5429
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1053 1622 3254 3686 4039 4701 1053
Size: 569 1632 432 353 662 1781
Fragments arranged by size:
1781 1632 662 569 432 353
AfeI AGC'GCT
Cuts at: 146 1074 146
Size: 928 4501
AflIII A'CryG_T
Cuts at: 2781 4581 4862 2781
Size: 1800 281 3348
Fragments arranged by size:
3348 1800 281
AleI CACnn'nnGTG
Cuts at: 664 1570 664
Size: 906 4523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2129 2129
Size: 5429
AlwNI CAG_nnn'CTG
Cuts at: 1392 2734 4172 1392
Size: 1342 1438 2649
Fragments arranged by size:
2649 1438 1342
ApaI G_GGCC'C
Cuts at: 528 1491 1910 2024 528
Size: 963 419 114 3933
Fragments arranged by size:
3933 963 419 114
ApaLI G'TGCA_C
Cuts at: 1021 4267 1021
Size: 3246 2183
ApoI r'AATT_y
Cuts at: 2433 4782 5038 2433
Size: 2349 256 2824
Fragments arranged by size:
2824 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5429
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5429
AvaI C'yCGr_G
Cuts at: 434 527 1568 1627 2025 2072 2153 2449 434
Size: 93 1041 59 398 47 81 296 3414
Fragments arranged by size:
3414 1041 398 296 93 81 59 47
BamHI G'GATC_C
Cuts at: 206 2454 206
Size: 2248 3181
BanI G'GyrC_C
Cuts at: 1039 1478 1772 2445 2859 3107 3142 1039
Size: 439 294 673 414 248 35 3326
Fragments arranged by size:
3326 673 439 414 294 248 35
BanII G_rGCy'C
Cuts at: 121 285 400 528 534 997 1187 1278 1491
Size: 164 115 128 6 463 190 91 213
Cuts at: 1491 1722 1910 2024 2082 2160 2249 2443 3473
Size: 231 188 114 58 78 89 194 1030
Cuts at: 3473 121
Size: 2077
Fragments arranged by size:
2077 1030 463 231 213 194 190 188
164 128 115 114 91 89 78 58
6
BbeI G_GCGC'C
Cuts at: 2863 3111 2863
Size: 248 5181
BbsI GAAGACnn'nnnn_
Cuts at: 311 311
Size: 5429
BbvCI CC'TCA_GC
Cuts at: 703 2347 703
Size: 1644 3785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 928 962 1333 1367 1618 1652 90
Size: 34 804 34 371 34 251 34 3867
Fragments arranged by size:
3867 804 371 251 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3327 4383 5429 3327
Size: 1056 1046 3327
Fragments arranged by size:
3327 1056 1046
BclI T'GATC_A
Cuts at: 2949 2949
Size: 5429
BfrBI ATG'CAT
Cuts at: 1669 1669
Size: 5429
BglI GCCn_nnn'nGGC
Cuts at: 579 1182 2164 2383 579
Size: 603 982 219 3625
Fragments arranged by size:
3625 982 603 219
BglII A'GATC_T
Cuts at: 2944 4981 2944
Size: 2037 3392
BlpI GC'TnA_GC
Cuts at: 1168 2541 1168
Size: 1373 4056
Bme1580I G_kGCm'C
Cuts at: 528 867 1025 1288 1481 1491 1910 2024 2379
Size: 339 158 263 193 10 419 114 355
Cuts at: 2379 3054 3147 4271 528
Size: 675 93 1124 1686
Fragments arranged by size:
1686 1124 675 419 355 339 263 193
158 114 93 10
BmgBI CAC'GTC
Cuts at: 1314 1842 1314
Size: 528 4901
BmrI ACTGGGnnnn_n'
Cuts at: 521 2641 2722 3057 5201 521
Size: 2120 81 335 2144 749
Fragments arranged by size:
2144 2120 749 335 81
BmtI G_CTAG'C
Cuts at: 1304 1304
Size: 5429
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 414 874 3796 414
Size: 460 2922 2047
Fragments arranged by size:
2922 2047 460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 1099 1360 1672 1706 2224 2233 2377 518
Size: 581 261 312 34 518 9 144 3570
Fragments arranged by size:
3570 581 518 312 261 144 34 9
Bpu10I CC'TnA_GC
Cuts at: 184 367 703 2347 184
Size: 183 336 1644 3266
Fragments arranged by size:
3266 1644 336 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2605 3484 4198 4496 2605
Size: 879 714 298 3538
Fragments arranged by size:
3538 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 586 1851 5133 586
Size: 1265 3282 882
Fragments arranged by size:
3282 1265 882
BsaAI yAC'GTr
Cuts at: 2782 2782
Size: 5429
BsaBI GATnn'nnATC
Cuts at: 6 2943 4980 6
Size: 2937 2037 455
Fragments arranged by size:
2937 2037 455
BsaHI Gr'CG_yC
Cuts at: 911 2860 3108 3810 5306 911
Size: 1949 248 702 1496 1034
Fragments arranged by size:
1949 1496 1034 702 248
BsaWI w'CCGG_w
Cuts at: 1127 2234 2618 3139 3817 4228 4375 4649 1127
Size: 1107 384 521 678 411 147 274 1907
Fragments arranged by size:
1907 1107 678 521 411 384 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 794 824 1015 1045 1243 1273 2032 2062 794
Size: 30 191 30 198 30 759 30 4161
Fragments arranged by size:
4161 759 198 191 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 269 754 863 2206 269
Size: 485 109 1343 3492
Fragments arranged by size:
3492 1343 485 109
BseYI C'CCAG_C
Cuts at: 393 1224 1229 1419 2378 2854 3612 4277 4685
Size: 831 5 190 959 476 758 665 408
Cuts at: 4685 393
Size: 1137
Fragments arranged by size:
1137 959 831 758 665 476 408 190
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 688 1286 1594 2302 198
Size: 490 598 308 708 3325
Fragments arranged by size:
3325 708 598 490 308
BsiEI CG_ry'CG
Cuts at: 104 2971 3017 4247 104
Size: 2867 46 1230 1286
Fragments arranged by size:
2867 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 121 285 368 997 1025 1722 2443 3221 3411
Size: 164 83 629 28 697 721 778 190
Cuts at: 3411 4271 121
Size: 860 1279
Fragments arranged by size:
1279 860 778 721 697 629 190 164
83 28
BsiWI C'GTAC_G
Cuts at: 472 472
Size: 5429
BsmI GAATG_Cn'
Cuts at: 1941 1941
Size: 5429
Bsp1286I G_dGCh'C
Cuts at: 121 285 368 400 528 534 867 997 1025
Size: 164 83 32 128 6 333 130 28
Cuts at: 1025 1187 1278 1288 1481 1491 1722 1910 2024
Size: 162 91 10 193 10 231 188 114
Cuts at: 2024 2082 2160 2249 2379 2443 3054 3147 3221
Size: 58 78 89 130 64 611 93 74
Cuts at: 3221 3411 3473 4271 121
Size: 190 62 798 1279
Fragments arranged by size:
1279 798 611 333 231 193 190 188
164 162 130 130 128 114 93 91
89 83 78 74 64 62 58 32
28 10 10 6
BspEI T'CCGG_A
Cuts at: 1127 1127
Size: 5429
BspHI T'CATG_A
Cuts at: 3861 3861
Size: 5429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 537 1457 2158 2479 2693 2995 3376 537
Size: 920 701 321 214 302 381 2590
Fragments arranged by size:
2590 920 701 381 321 302 214
BsrBI CCG'CTC
Cuts at: 50 408 570 1089 3721 3775 5259 5424 50
Size: 358 162 519 2632 54 1484 165 55
Fragments arranged by size:
2632 1484 519 358 165 162 55 54
BsrDI GCAATG_nn'
Cuts at: 1669 3341 1669
Size: 1672 3757
BsrFI r'CCGG_y
Cuts at: 1052 1481 2102 3427 3608 1052
Size: 429 621 1325 181 2873
Fragments arranged by size:
2873 1325 621 429 181
BssSI C'ACGA_G
Cuts at: 1658 1700 3700 4408 1658
Size: 42 2000 708 2679
Fragments arranged by size:
2679 2000 708 42
BstAPI GCAn_nnn'nTGC
Cuts at: 691 1392 1581 2806 691
Size: 701 189 1225 3314
Fragments arranged by size:
3314 1225 701 189
BstBI TT'CG_AA
Cuts at: 3790 3790
Size: 5429
BstXI CCAn_nnnn'nTGG
Cuts at: 1650 1650
Size: 5429
BstYI r'GATC_y
Cuts at: 1 206 225 1124 2454 2921 2944 3279 3525
Size: 205 19 899 1330 467 23 335 246
Cuts at: 3525 3831 3843 3929 4981 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1330 1052 899 467 449 335 306 246
205 86 23 19 12
BtgI C'CryG_G
Cuts at: 458 1713 2311 458
Size: 1255 598 3576
Fragments arranged by size:
3576 1255 598
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3379 3560 3723 3379
Size: 181 163 5085
Fragments arranged by size:
5085 181 163
BtsI GCAGTG_nn'
Cuts at: 2672 2672
Size: 5429
DraI TTT'AAA
Cuts at: 2428 2428
Size: 5429
DraIII CAC_nnn'GTG
Cuts at: 1474 1863 1892 1474
Size: 389 29 5011
Fragments arranged by size:
5011 389 29
DrdI GACnn_nn'nnGTC
Cuts at: 3135 4479 3135
Size: 1344 4085
EaeI y'GGCC_r
Cuts at: 139 455 696 826 962 1516 1710 3014 3188
Size: 316 241 130 136 554 194 1304 174
Cuts at: 3188 3579 3606 4951 139
Size: 391 27 1345 617
Fragments arranged by size:
1345 1304 617 554 391 316 241 194
174 136 130 27
EagI C'GGCC_G
Cuts at: 3014 3014
Size: 5429
EarI CTCTTCn'nnn_
Cuts at: 1879 2183 3452 3662 1879
Size: 304 1269 210 3646
Fragments arranged by size:
3646 1269 304 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1324 3637 4379 4525 5239 1324
Size: 2313 742 146 714 1514
Fragments arranged by size:
2313 1514 742 714 146
EcoICRI GAG'CTC
Cuts at: 119 283 995 1720 2441 119
Size: 164 712 725 721 3107
Fragments arranged by size:
3107 725 721 712 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 1053 1099 1360 1622 1672 1706 2224 2233
Size: 535 46 261 262 50 34 518 9
Cuts at: 2233 2377 3254 3686 4039 4701 518
Size: 144 877 432 353 662 1246
Fragments arranged by size:
1246 877 662 535 518 432 353 262
261 144 50 46 34 9
EcoNI CCTnn'n_nnAGG
Cuts at: 333 333
Size: 5429
EcoO109I rG'GnC_Cy
Cuts at: 339 524 672 975 1488 1815 2020 2021 2568
Size: 185 148 303 513 327 205 1 547
Cuts at: 2568 339
Size: 3200
Fragments arranged by size:
3200 547 513 327 303 205 185 148
1
EcoRI G'AATT_C
Cuts at: 2433 2433
Size: 5429
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3448 3448
Size: 5429
FspI TGC'GCA
Cuts at: 3210 3210
Size: 5429
HaeII r_GCGC'y
Cuts at: 148 1076 1359 1413 2863 3111 4341 5112 148
Size: 928 283 54 1450 248 1230 771 465
Fragments arranged by size:
1450 1230 928 771 465 283 248 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 878 910 1831 1863 2032 2064 3271 3301 3303
Size: 32 921 32 169 32 1207 30 2
Cuts at: 3303 3333 878
Size: 30 2974
Fragments arranged by size:
2974 1207 921 169 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2468 2468
Size: 5429
KasI G'GCGC_C
Cuts at: 2859 3107 2859
Size: 248 5181
KpnI G_GTAC'C
Cuts at: 1043 1776 2449 1043
Size: 733 673 4023
Fragments arranged by size:
4023 733 673
MluI A'CGCG_T
Cuts at: 4862 4862
Size: 5429
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1064 1307 1436 4188 4372 1064
Size: 243 129 2752 184 2121
Fragments arranged by size:
2752 2121 243 184 129
MscI TGG'CCA
Cuts at: 698 964 3190 698
Size: 266 2226 2937
Fragments arranged by size:
2937 2226 266
MslI CAynn'nnrTG
Cuts at: 563 664 836 1570 1840 3418 3545 563
Size: 101 172 734 270 1578 127 2447
Fragments arranged by size:
2447 1578 734 270 172 127 101
MspA1I CmG'CkG
Cuts at: 519 1578 1756 1925 2339 2382 2540 2854 3214
Size: 1059 178 169 414 43 158 314 360
Cuts at: 3214 3998 4243 519
Size: 784 245 1705
Fragments arranged by size:
1705 1059 784 414 360 314 245 178
169 158 43
NaeI GCC'GGC
Cuts at: 3610 3610
Size: 5429
NarI GG'CG_CC
Cuts at: 2860 3108 2860
Size: 248 5181
NdeI CA'TA_TG
Cuts at: 663 663
Size: 5429
NgoMIV G'CCGG_C
Cuts at: 3608 3608
Size: 5429
NheI G'CTAG_C
Cuts at: 1300 1300
Size: 5429
NsiI A_TGCA'T
Cuts at: 1671 1671
Size: 5429
NspI r_CATG'y
Cuts at: 1669 2482 2982 4585 1669
Size: 813 500 1603 2513
Fragments arranged by size:
2513 1603 813 500
PasI CC'CwG_GG
Cuts at: 1777 2017 1777
Size: 240 5189
PciI A'CATG_T
Cuts at: 4581 4581
Size: 5429
PflMI CCAn_nnn'nTGG
Cuts at: 1735 1892 1735
Size: 157 5272
PfoI T'CCnGG_A
Cuts at: 202 1649 1976 202
Size: 1447 327 3655
Fragments arranged by size:
3655 1447 327
PmeI GTTT'AAAC
Cuts at: 2428 2428
Size: 5429
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 3875
Size: 32 5397
PpuMI rG'GwC_Cy
Cuts at: 339 975 339
Size: 636 4793
PspOMI G'GGCC_C
Cuts at: 524 1487 1906 2020 524
Size: 963 419 114 3933
Fragments arranged by size:
3933 963 419 114
PspXI vC'TCGA_Gb
Cuts at: 1627 1627
Size: 5429
PstI C_TGCA'G
Cuts at: 1118 1614 1752 2308 2476 3161 1118
Size: 496 138 556 168 685 3386
Fragments arranged by size:
3386 685 556 496 168 138
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5429
PvuII CAG'CTG
Cuts at: 1578 2382 2854 3214 1578
Size: 804 472 360 3793
Fragments arranged by size:
3793 804 472 360
RsrII CG'GwC_CG
Cuts at: 3624 3624
Size: 5429
SacI G_AGCT'C
Cuts at: 121 285 997 1722 2443 121
Size: 164 712 725 721 3107
Fragments arranged by size:
3107 725 721 712 164
SalI G'TCGA_C
Cuts at: 2466 2466
Size: 5429
SanDI GG'GwC_CC
Cuts at: 339 975 339
Size: 636 4793
SapI GCTCTTCn'nnn_
Cuts at: 3452 3662 3452
Size: 210 5219
SbfI CC_TGCA'GG
Cuts at: 2476 2476
Size: 5429
ScaI AGT'ACT
Cuts at: 4946 4946
Size: 5429
SfcI C'TryA_G
Cuts at: 33 242 333 1114 1291 1610 1748 2084 2304
Size: 209 91 781 177 319 138 336 220
Cuts at: 2304 2472 3157 4125 4316 33
Size: 168 685 968 191 1146
Fragments arranged by size:
1146 968 781 685 336 319 220 209
191 177 168 138 91
SfoI GGC'GCC
Cuts at: 2861 3109 2861
Size: 248 5181
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5429
SgrAI Cr'CCGG_yG
Cuts at: 1481 1481
Size: 5429
SmaI CCC'GGG
Cuts at: 529 2027 2074 2451 529
Size: 1498 47 377 3507
Fragments arranged by size:
3507 1498 377 47
SmlI C'TyrA_G
Cuts at: 1627 2584 3499 4213 4475 1627
Size: 957 915 714 262 2581
Fragments arranged by size:
2581 957 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5429
SphI G_CATG'C
Cuts at: 2482 2982 2482
Size: 500 4929
SrfI GCCC'GGGC
Cuts at: 529 529
Size: 5429
StuI AGG'CCT
Cuts at: 1633 1902 1957 1633
Size: 269 55 5105
Fragments arranged by size:
5105 269 55
StyI C'CwwG_G
Cuts at: 2563 2917 2563
Size: 354 5075
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1306 3012 3145 3604 3813 1306
Size: 1706 133 459 209 2922
Fragments arranged by size:
2922 1706 459 209 133
TatI w'GTAC_w
Cuts at: 722 1436 4944 722
Size: 714 3508 1207
Fragments arranged by size:
3508 1207 714
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 794 826 1015 1047 1243 1275 4946 4978 794
Size: 32 189 32 196 32 3671 32 1245
Fragments arranged by size:
3671 1245 196 189 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1843 3226 1843
Size: 1383 4046
XbaI T'CTAG_A
Cuts at: 2460 2460
Size: 5429
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 456 2924 456
Size: 2468 2961
XhoI C'TCGA_G
Cuts at: 1627 1627
Size: 5429
XmaI C'CCGG_G
Cuts at: 527 2025 2072 2449 527
Size: 1498 47 377 3507
Fragments arranged by size:
3507 1498 377 47
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AloI AscI AvrII BaeI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NotI
NruI PacI PmlI PshAI PsiI PsrI SacII SexAI
SfiI SnaBI SspI SwaI XmnI ZraI