Restriction map for
pF1KA1405
(Circular) (Six-base) MAPSORT of: pF1KA1405.seq Check: 9751 from: 1 to: 6200
pF1KA1405 6200 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1236 1257 1491 1880 2316 2685 1236
Size: 21 234 389 436 369 4751
Fragments arranged by size:
4751 436 389 369 234 21
AatII G_ACGT'C
Cuts at: 1448 2027 1448
Size: 579 5621
AccI GT'mk_AC
Cuts at: 2507 3238 2507
Size: 731 5469
Acc65I G'GTAC_C
Cuts at: 3216 3216
Size: 6200
AclI AA'CG_TT
Cuts at: 6040 6040
Size: 6200
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 2416 4025 4457 4810 5472 610
Size: 1806 1609 432 353 662 1338
Fragments arranged by size:
1806 1609 1338 662 432 353
AfeI AGC'GCT
Cuts at: 1557 2422 1557
Size: 865 5335
AflIII A'CryG_T
Cuts at: 467 3552 5352 5633 467
Size: 3085 1800 281 1034
Fragments arranged by size:
3085 1800 1034 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2066 2066
Size: 6200
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 678 709 710 741 678
Size: 31 1 31 6137
Fragments arranged by size:
6137 31 31 1
AlwNI CAG_nnn'CTG
Cuts at: 417 582 1261 1492 2179 2331 2688 2706 2720
Size: 165 679 231 687 152 357 18 14
Cuts at: 2720 3505 4943 417
Size: 785 1438 1674
Fragments arranged by size:
1674 1438 785 687 679 357 231 165
152 18 14
ApaLI G'TGCA_C
Cuts at: 643 5038 643
Size: 4395 1805
ApoI r'AATT_y
Cuts at: 3204 5553 5809 3204
Size: 2349 256 3595
Fragments arranged by size:
3595 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6200
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6200
AvaI C'yCGr_G
Cuts at: 231 1397 1849 3121 3220 231
Size: 1166 452 1272 99 3211
Fragments arranged by size:
3211 1272 1166 452 99
BamHI G'GATC_C
Cuts at: 423 3225 423
Size: 2802 3398
BanI G'GyrC_C
Cuts at: 235 274 361 1248 1868 1917 3216 3630 3878
Size: 39 87 887 620 49 1299 414 248
Cuts at: 3878 3913 235
Size: 35 2522
Fragments arranged by size:
2522 1299 887 620 414 248 87 49
39 35
BanII G_rGCy'C
Cuts at: 1216 1720 1784 2075 2234 3214 4244 1216
Size: 504 64 291 159 980 1030 3172
Fragments arranged by size:
3172 1030 980 504 291 159 64
BbeI G_GCGC'C
Cuts at: 239 278 3634 3882 239
Size: 39 3356 248 2557
Fragments arranged by size:
3356 2557 248 39
BbsI GAAGACnn'nnnn_
Cuts at: 1421 1599 1421
Size: 178 6022
BbvCI CC'TCA_GC
Cuts at: 1523 2992 3043 1523
Size: 1469 51 4680
Fragments arranged by size:
4680 1469 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 751 785 1378 1412 2046 2080 751
Size: 34 593 34 634 34 4871
Fragments arranged by size:
4871 634 593 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4098 5154 6200 4098
Size: 1056 1046 4098
Fragments arranged by size:
4098 1056 1046
BclI T'GATC_A
Cuts at: 3720 3720
Size: 6200
BglI GCCn_nnn'nGGC
Cuts at: 118 386 1099 1975 2101 2209 2218 118
Size: 268 713 876 126 108 9 4100
Fragments arranged by size:
4100 876 713 268 126 108 9
BglII A'GATC_T
Cuts at: 305 530 2133 2598 3715 5752 305
Size: 225 1603 465 1117 2037 753
Fragments arranged by size:
2037 1603 1117 753 465 225
BlpI GC'TnA_GC
Cuts at: 2222 3312 2222
Size: 1090 5110
Bme1580I G_kGCm'C
Cuts at: 647 1253 1871 1922 3825 3918 5042 647
Size: 606 618 51 1903 93 1124 1805
Fragments arranged by size:
1903 1805 1124 618 606 93 51
BmgBI CAC'GTC
Cuts at: 294 960 294
Size: 666 5534
BmrI ACTGGGnnnn_n'
Cuts at: 207 651 925 1015 1625 2069 2499 2818 2849
Size: 444 274 90 610 444 430 319 31
Cuts at: 2849 3412 3493 3828 5972 207
Size: 563 81 335 2144 435
Fragments arranged by size:
2144 610 563 444 444 435 430 335
319 274 90 81 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1749 1800 2682 4567 1749
Size: 51 882 1885 3382
Fragments arranged by size:
3382 1885 882 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 604 1429 1483 1647 1704 2089 2572 2641
Size: 57 825 54 164 57 385 483 69
Cuts at: 2641 2710 2758 547
Size: 69 48 3989
Fragments arranged by size:
3989 825 483 385 164 69 69 57
57 54 48
Bpu10I CC'TnA_GC
Cuts at: 1523 2198 2228 2992 3043 1523
Size: 675 30 764 51 4680
Fragments arranged by size:
4680 764 675 51 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 1171 3376 4255 4969 5267 862
Size: 309 2205 879 714 298 1795
Fragments arranged by size:
2205 1795 879 714 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 1803 5904 1803
Size: 4101 2099
BsaAI yAC'GTr
Cuts at: 285 468 618 3553 285
Size: 183 150 2935 2932
Fragments arranged by size:
2935 2932 183 150
BsaBI GATnn'nnATC
Cuts at: 6 3714 5751 6
Size: 3708 2037 455
Fragments arranged by size:
3708 2037 455
BsaHI Gr'CG_yC
Cuts at: 236 275 341 1445 2024 3002 3631 3879 4581
Size: 39 66 1104 579 978 629 248 702
Cuts at: 4581 6077 236
Size: 1496 359
Fragments arranged by size:
1496 1104 978 702 629 579 359 248
66 39
BsaWI w'CCGG_w
Cuts at: 3389 3910 4588 4999 5146 5420 3389
Size: 521 678 411 147 274 4169
Fragments arranged by size:
4169 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1267 1297 1627 1657 2925 2955 1267
Size: 30 330 30 1268 30 4512
Fragments arranged by size:
4512 1268 330 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1288 1354 1480 1792 1828 1918 1977 2917
Size: 102 66 126 312 36 90 59 940
Cuts at: 2917 1186
Size: 4469
Fragments arranged by size:
4469 940 312 126 102 90 66 59
36
BseYI C'CCAG_C
Cuts at: 1216 1864 1875 2762 3101 3625 4383 5048 5456
Size: 648 11 887 339 524 758 665 408
Cuts at: 5456 1216
Size: 1960
Fragments arranged by size:
1960 887 758 665 648 524 408 339
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 1285 2062 2719 509
Size: 776 777 657 3990
Fragments arranged by size:
3990 777 776 657
BsiEI CG_ry'CG
Cuts at: 104 701 3742 3788 5018 104
Size: 597 3041 46 1230 1286
Fragments arranged by size:
3041 1286 1230 597 46
BsiHKAI G_wGCw'C
Cuts at: 467 599 647 668 3214 3992 4182 5042 467
Size: 132 48 21 2546 778 190 860 1625
Fragments arranged by size:
2546 1625 860 778 190 132 48 21
Bsp1286I G_dGCh'C
Cuts at: 467 599 647 668 1216 1253 1720 1784 1871
Size: 132 48 21 548 37 467 64 87
Cuts at: 1871 1922 2075 2234 3214 3825 3918 3992 4182
Size: 51 153 159 980 611 93 74 190
Cuts at: 4182 4244 5042 467
Size: 62 798 1625
Fragments arranged by size:
1625 980 798 611 548 467 190 159
153 132 93 87 74 64 62 51
48 37 21
BspHI T'CATG_A
Cuts at: 4632 4632
Size: 6200
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1236 1257 1481 1491 1880 2034 2072 2316 2349
Size: 21 224 10 389 154 38 244 33
Cuts at: 2349 2685 3250 3464 3766 4147 1236
Size: 336 565 214 302 381 3289
Fragments arranged by size:
3289 565 389 381 336 302 244 224
214 154 38 33 21 10
BsrBI CCG'CTC
Cuts at: 50 783 843 873 4492 4546 6030 6195 50
Size: 733 60 30 3619 54 1484 165 55
Fragments arranged by size:
3619 1484 733 165 60 55 54 30
BsrDI GCAATG_nn'
Cuts at: 927 2948 4112 927
Size: 2021 1164 3015
Fragments arranged by size:
3015 2021 1164
BsrFI r'CCGG_y
Cuts at: 2238 2651 4198 4379 2238
Size: 413 1547 181 4059
Fragments arranged by size:
4059 1547 413 181
BssHII G'CGCG_C
Cuts at: 203 205 207 203
Size: 2 2 6196
Fragments arranged by size:
6196 2 2
BssSI C'ACGA_G
Cuts at: 4471 5179 4471
Size: 708 5492
BstAPI GCAn_nnn'nTGC
Cuts at: 417 642 1917 2328 3577 417
Size: 225 1275 411 1249 3040
Fragments arranged by size:
3040 1275 1249 411 225
BstBI TT'CG_AA
Cuts at: 4561 4561
Size: 6200
BstYI r'GATC_y
Cuts at: 1 305 423 530 2133 2598 2789 2987 3225
Size: 304 118 107 1603 465 191 198 238
Cuts at: 3225 3692 3715 4050 4296 4602 4614 4700 5752
Size: 467 23 335 246 306 12 86 1052
Cuts at: 5752 1
Size: 449
Fragments arranged by size:
1603 1052 467 465 449 335 306 304
246 238 198 191 118 107 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 1775 2126 1775
Size: 351 5849
BtgI C'CryG_G
Cuts at: 107 185 773 863 1994 107
Size: 78 588 90 1131 4313
Fragments arranged by size:
4313 1131 588 90 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4150 4331 4494 4150
Size: 181 163 5856
Fragments arranged by size:
5856 181 163
BtsI GCAGTG_nn'
Cuts at: 1421 2291 3189 3443 1421
Size: 870 898 254 4178
Fragments arranged by size:
4178 898 870 254
ClaI AT'CG_AT
Cuts at: 2630 2630
Size: 6200
DraI TTT'AAA
Cuts at: 3199 3199
Size: 6200
DraIII CAC_nnn'GTG
Cuts at: 642 651 642
Size: 9 6191
DrdI GACnn_nn'nnGTC
Cuts at: 3906 5250 3906
Size: 1344 4856
EaeI y'GGCC_r
Cuts at: 378 1976 1997 2240 2639 3785 3959 4350 4377
Size: 1598 21 243 399 1146 174 391 27
Cuts at: 4377 5722 378
Size: 1345 856
Fragments arranged by size:
1598 1345 1146 856 399 391 243 174
27 21
EagI C'GGCC_G
Cuts at: 3785 3785
Size: 6200
EarI CTCTTCn'nnn_
Cuts at: 1343 2251 3004 4223 4433 1343
Size: 908 753 1219 210 3110
Fragments arranged by size:
3110 1219 908 753 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 416 2638 3106 4408 5150 5296 6010 416
Size: 2222 468 1302 742 146 714 606
Fragments arranged by size:
2222 1302 742 714 606 468 146
EcoICRI GAG'CTC
Cuts at: 3212 3212
Size: 6200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 604 610 1429 1483 1647 1704 2089 2416
Size: 57 6 819 54 164 57 385 327
Cuts at: 2416 2572 2641 2710 2758 4025 4457 4810 5472
Size: 156 69 69 48 1267 432 353 662
Cuts at: 5472 547
Size: 1275
Fragments arranged by size:
1275 1267 819 662 432 385 353 327
164 156 69 69 57 57 54 48
6
EcoNI CCTnn'n_nnAGG
Cuts at: 1477 1516 1852 2692 1477
Size: 39 336 840 4985
Fragments arranged by size:
4985 840 336 39
EcoO109I rG'GnC_Cy
Cuts at: 414 455 552 1137 1947 2034 2046 2202 2391
Size: 41 97 585 810 87 12 156 189
Cuts at: 2391 3087 3339 414
Size: 696 252 3275
Fragments arranged by size:
3275 810 696 585 252 189 156 97
87 41 12
EcoRI G'AATT_C
Cuts at: 3204 3204
Size: 6200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4219 4219
Size: 6200
FspI TGC'GCA
Cuts at: 1432 3981 1432
Size: 2549 3651
HaeII r_GCGC'y
Cuts at: 239 278 937 951 1356 1559 2424 3634 3882
Size: 39 659 14 405 203 865 1210 248
Cuts at: 3882 5112 5883 239
Size: 1230 771 556
Fragments arranged by size:
1230 1210 865 771 659 556 405 248
203 39 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 309 341 415 431 447 463 832 864 1378
Size: 32 74 16 16 16 369 32 514
Cuts at: 1378 1410 1516 1548 1608 1627 1640 1659 2125
Size: 32 106 32 60 19 13 19 466
Cuts at: 2125 2157 2583 2590 2615 2622 2781 2813 4042
Size: 32 426 7 25 7 159 32 1229
Cuts at: 4042 4072 4074 4104 309
Size: 30 2 30 2405
Fragments arranged by size:
2405 1229 514 466 426 369 159 106
74 60 32 32 32 32 32 32
30 30 25 19 19 16 16 16
13 7 7 2
HincII GTy'rAC
Cuts at: 3239 3239
Size: 6200
HindIII A'AGCT_T
Cuts at: 2569 2569
Size: 6200
KasI G'GCGC_C
Cuts at: 235 274 3630 3878 235
Size: 39 3356 248 2557
Fragments arranged by size:
3356 2557 248 39
KpnI G_GTAC'C
Cuts at: 3220 3220
Size: 6200
MluI A'CGCG_T
Cuts at: 5633 5633
Size: 6200
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1811 2171 2330 2911 4959 5143 1811
Size: 360 159 581 2048 184 2868
Fragments arranged by size:
2868 2048 581 360 184 159
MscI TGG'CCA
Cuts at: 1978 1999 2641 3961 1978
Size: 21 642 1320 4217
Fragments arranged by size:
4217 1320 642 21
MslI CAynn'nnrTG
Cuts at: 712 763 1426 2278 4189 4316 712
Size: 51 663 852 1911 127 2596
Fragments arranged by size:
2596 1911 852 663 127 51
MspA1I CmG'CkG
Cuts at: 159 330 392 1329 1864 2248 2304 2346 2448
Size: 171 62 937 535 384 56 42 102
Cuts at: 2448 2483 2619 3047 3311 3625 3985 4769 5014
Size: 35 136 428 264 314 360 784 245
Cuts at: 5014 159
Size: 1345
Fragments arranged by size:
1345 937 784 535 428 384 360 314
264 245 171 136 102 62 56 42
35
NaeI GCC'GGC
Cuts at: 2240 2653 4381 2240
Size: 413 1728 4059
Fragments arranged by size:
4059 1728 413
NarI GG'CG_CC
Cuts at: 236 275 3631 3879 236
Size: 39 3356 248 2557
Fragments arranged by size:
3356 2557 248 39
NcoI C'CATG_G
Cuts at: 107 107
Size: 6200
NdeI CA'TA_TG
Cuts at: 1440 1440
Size: 6200
NgoMIV G'CCGG_C
Cuts at: 2238 2651 4379 2238
Size: 413 1728 4059
Fragments arranged by size:
4059 1728 413
NruI TCG'CGA
Cuts at: 1160 1160
Size: 6200
NspI r_CATG'y
Cuts at: 3253 3753 5356 3253
Size: 500 1603 4097
Fragments arranged by size:
4097 1603 500
PasI CC'CwG_GG
Cuts at: 452 1768 452
Size: 1316 4884
PciI A'CATG_T
Cuts at: 5352 5352
Size: 6200
PflMI CCAn_nnn'nTGG
Cuts at: 186 186
Size: 6200
PfoI T'CCnGG_A
Cuts at: 548 1334 1819 1835 2519 2687 548
Size: 786 485 16 684 168 4061
Fragments arranged by size:
4061 786 684 485 168 16
PmeI GTTT'AAAC
Cuts at: 3199 3199
Size: 6200
PmlI CAC'GTG
Cuts at: 285 468 285
Size: 183 6017
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 454 486 4646 4678 454
Size: 32 4160 32 1976
Fragments arranged by size:
4160 1976 32 32
PpuMI rG'GwC_Cy
Cuts at: 552 1137 1947 2034 2046 2391 552
Size: 585 810 87 12 345 4361
Fragments arranged by size:
4361 810 585 345 87 12
PspXI vC'TCGA_Gb
Cuts at: 3121 3121
Size: 6200
PstI C_TGCA'G
Cuts at: 998 1502 1928 1946 2345 2465 2606 2877 3247
Size: 504 426 18 399 120 141 271 370
Cuts at: 3247 3932 998
Size: 685 3266
Fragments arranged by size:
3266 685 504 426 399 370 271 141
120 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6200
PvuII CAG'CTG
Cuts at: 1329 2248 2346 2448 2619 3047 3625 3985 1329
Size: 919 98 102 171 428 578 360 3544
Fragments arranged by size:
3544 919 578 428 360 171 102 98
RsrII CG'GwC_CG
Cuts at: 4395 4395
Size: 6200
SacI G_AGCT'C
Cuts at: 3214 3214
Size: 6200
SalI G'TCGA_C
Cuts at: 3237 3237
Size: 6200
SapI GCTCTTCn'nnn_
Cuts at: 1343 3004 4223 4433 1343
Size: 1661 1219 210 3110
Fragments arranged by size:
3110 1661 1219 210
SbfI CC_TGCA'GG
Cuts at: 1502 1928 1946 3247 1502
Size: 426 18 1301 4455
Fragments arranged by size:
4455 1301 426 18
ScaI AGT'ACT
Cuts at: 5717 5717
Size: 6200
SexAI A'CCwGG_T
Cuts at: 818 818
Size: 6200
SfcI C'TryA_G
Cuts at: 33 994 1498 1924 1942 2341 2461 2602 2727
Size: 961 504 426 18 399 120 141 125
Cuts at: 2727 2873 2913 3243 3928 4896 5087 33
Size: 146 40 330 685 968 191 1146
Fragments arranged by size:
1146 968 961 685 504 426 399 330
191 146 141 125 120 40 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 2209 2209
Size: 6200
SfoI GGC'GCC
Cuts at: 237 276 3632 3880 237
Size: 39 3356 248 2557
Fragments arranged by size:
3356 2557 248 39
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6200
SmaI CCC'GGG
Cuts at: 233 3222 233
Size: 2989 3211
SmlI C'TyrA_G
Cuts at: 877 1186 3121 3355 4270 4984 5246 877
Size: 309 1935 234 915 714 262 1831
Fragments arranged by size:
1935 1831 915 714 309 262 234
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6200
SphI G_CATG'C
Cuts at: 3253 3753 3253
Size: 500 5700
StuI AGG'CCT
Cuts at: 1940 2124 3041 1940
Size: 184 917 5099
Fragments arranged by size:
5099 917 184
StyI C'CwwG_G
Cuts at: 107 558 1142 1601 1682 2030 3146 3334 3688
Size: 451 584 459 81 348 1116 188 354
Cuts at: 3688 107
Size: 2619
Fragments arranged by size:
2619 1116 584 459 451 354 348 188
81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 614 687 2356 2481 3093 3097 3783 3916 4375
Size: 73 1669 125 612 4 686 133 459
Cuts at: 4375 4584 614
Size: 209 2230
Fragments arranged by size:
2230 1669 686 612 459 209 133 125
73 4
TatI w'GTAC_w
Cuts at: 5715 5715
Size: 6200
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2925 2957 5717 5749 2925
Size: 32 2760 32 3376
Fragments arranged by size:
3376 2760 32 32
Tth111I GACn'n_nGTC
Cuts at: 292 1639 3997 292
Size: 1347 2358 2495
Fragments arranged by size:
2495 2358 1347
XbaI T'CTAG_A
Cuts at: 3231 3231
Size: 6200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 579 1026 3695 579
Size: 447 2669 3084
Fragments arranged by size:
3084 2669 447
XhoI C'TCGA_G
Cuts at: 3121 3121
Size: 6200
XmaI C'CCGG_G
Cuts at: 231 3220 231
Size: 2989 3211
XmnI GAAnn'nnTTC
Cuts at: 400 400
Size: 6200
ZraI GAC'GTC
Cuts at: 1446 2025 1446
Size: 579 5621
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI ApaI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BsmBI BspEI BsrGI BstEII
BstXI BstZ17I CspCI EcoRV FseI FspAI HpaI MfeI
NheI NotI NsiI PacI PshAI PsiI PspOMI PsrI
SacII SanDI SgrAI SnaBI SrfI SspI SwaI