Restriction map for
pF1KA1411
(Circular) (Six-base) MAPSORT of: pF1KA1411.seq Check: 2462 from: 1 to: 7658
pF1KA1411 7658 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AccI GT'mk_AC
Cuts at: 1513 4696 1513
Size: 3183 4475
Acc65I G'GTAC_C
Cuts at: 4674 4674
Size: 7658
AclI AA'CG_TT
Cuts at: 7498 7498
Size: 7658
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 1382 1384 1460 3322 3712 3766 3860 5483
Size: 1248 2 76 1862 390 54 94 1623
Cuts at: 5483 5915 6268 6930 134
Size: 432 353 662 862
Fragments arranged by size:
1862 1623 1248 862 662 432 390 353
94 76 54 2
AflIII A'CryG_T
Cuts at: 3268 4276 5010 6810 7091 3268
Size: 1008 734 1800 281 3835
Fragments arranged by size:
3835 1800 1008 734 281
AhdI GACnn_n'nnGTC
Cuts at: 4010 4443 4010
Size: 433 7225
AlwNI CAG_nnn'CTG
Cuts at: 1071 1360 4963 6401 1071
Size: 289 3603 1438 2328
Fragments arranged by size:
3603 2328 1438 289
ApaLI G'TGCA_C
Cuts at: 1233 3868 6496 1233
Size: 2635 2628 2395
Fragments arranged by size:
2635 2628 2395
ApoI r'AATT_y
Cuts at: 133 212 224 326 429 1388 1938 2554 2667
Size: 79 12 102 103 959 550 616 113
Cuts at: 2667 2847 3009 3625 4618 4662 7011 7267 133
Size: 180 162 616 993 44 2349 256 524
Fragments arranged by size:
2349 993 959 616 616 550 524 256
180 162 113 103 102 79 44 12
AseI AT'TA_AT
Cuts at: 20 1633 20
Size: 1613 6045
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7658
AvaI C'yCGr_G
Cuts at: 4678 4678
Size: 7658
BamHI G'GATC_C
Cuts at: 1758 4369 4683 1758
Size: 2611 314 4733
Fragments arranged by size:
4733 2611 314
BanI G'GyrC_C
Cuts at: 1495 4674 5088 5336 5371 1495
Size: 3179 414 248 35 3782
Fragments arranged by size:
3782 3179 414 248 35
BanII G_rGCy'C
Cuts at: 4672 5702 4672
Size: 1030 6628
BbeI G_GCGC'C
Cuts at: 5092 5340 5092
Size: 248 7410
BbsI GAAGACnn'nnnn_
Cuts at: 673 1370 673
Size: 697 6961
BbvCI CC'TCA_GC
Cuts at: 2522 2522
Size: 7658
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2724 2758 2724
Size: 34 7624
BciVI GTATCCnnnnn_n'
Cuts at: 2934 5556 6612 7658 2934
Size: 2622 1056 1046 2934
Fragments arranged by size:
2934 2622 1056 1046
BclI T'GATC_A
Cuts at: 2040 5178 2040
Size: 3138 4520
BfrBI ATG'CAT
Cuts at: 616 4540 616
Size: 3924 3734
BglII A'GATC_T
Cuts at: 1308 3894 4454 5173 7210 1308
Size: 2586 560 719 2037 1756
Fragments arranged by size:
2586 2037 1756 719 560
BlpI GC'TnA_GC
Cuts at: 4770 4770
Size: 7658
Bme1580I G_kGCm'C
Cuts at: 1237 3872 5283 5376 6500 1237
Size: 2635 1411 93 1124 2395
Fragments arranged by size:
2635 2395 1411 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2084 4870 4951 5286 7430 2084
Size: 2786 81 335 2144 2312
Fragments arranged by size:
2786 2312 2144 335 81
BmtI G_CTAG'C
Cuts at: 859 1829 859
Size: 970 6688
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6025 6025
Size: 7658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1485 1853 949
Size: 536 368 6754
Fragments arranged by size:
6754 536 368
Bpu10I CC'TnA_GC
Cuts at: 2522 2522
Size: 7658
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 4834 5713 6427 6725 439
Size: 4395 879 714 298 1372
Fragments arranged by size:
4395 1372 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4231 7362 4231
Size: 3131 4527
BsaAI yAC'GTr
Cuts at: 5011 5011
Size: 7658
BsaBI GATnn'nnATC
Cuts at: 6 1307 5172 7209 6
Size: 1301 3865 2037 455
Fragments arranged by size:
3865 2037 1301 455
BsaHI Gr'CG_yC
Cuts at: 5089 5337 6039 7535 5089
Size: 248 702 1496 5212
Fragments arranged by size:
5212 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4847 5368 6046 6457 6604 6878 4847
Size: 521 678 411 147 274 5627
Fragments arranged by size:
5627 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3379 3379
Size: 7658
BseYI C'CCAG_C
Cuts at: 5083 5841 6506 6914 5083
Size: 758 665 408 5827
Fragments arranged by size:
5827 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 3563 3911 4562 511
Size: 3052 348 651 3607
Fragments arranged by size:
3607 3052 651 348
BsiEI CG_ry'CG
Cuts at: 104 5200 5246 6476 104
Size: 5096 46 1230 1286
Fragments arranged by size:
5096 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1124 1237 3872 4212 4672 5450 5640 6500 1124
Size: 113 2635 340 460 778 190 860 2282
Fragments arranged by size:
2635 2282 860 778 460 340 190 113
BsmI GAATG_Cn'
Cuts at: 495 1156 2698 495
Size: 661 1542 5455
Fragments arranged by size:
5455 1542 661
Bsp1286I G_dGCh'C
Cuts at: 1124 1237 3872 4212 4672 5283 5376 5450 5640
Size: 113 2635 340 460 611 93 74 190
Cuts at: 5640 5702 6500 1124
Size: 62 798 2282
Fragments arranged by size:
2635 2282 798 611 460 340 190 113
93 74 62
BspHI T'CATG_A
Cuts at: 294 6090 294
Size: 5796 1862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1497 2325 4708 4922 5224 5605 1497
Size: 828 2383 214 302 381 3550
Fragments arranged by size:
3550 2383 828 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5950 6004 7488 7653 50
Size: 5900 54 1484 165 55
Fragments arranged by size:
5900 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 131 791 1190 3294 4540 5570 131
Size: 660 399 2104 1246 1030 2219
Fragments arranged by size:
2219 2104 1246 1030 660 399
BsrFI r'CCGG_y
Cuts at: 5656 5837 5656
Size: 181 7477
BsrGI T'GTAC_A
Cuts at: 332 3773 332
Size: 3441 4217
BssSI C'ACGA_G
Cuts at: 3584 5929 6637 3584
Size: 2345 708 4605
Fragments arranged by size:
4605 2345 708
BstAPI GCAn_nnn'nTGC
Cuts at: 5035 5035
Size: 7658
BstBI TT'CG_AA
Cuts at: 6019 6019
Size: 7658
BstXI CCAn_nnnn'nTGG
Cuts at: 1422 1422
Size: 7658
BstYI r'GATC_y
Cuts at: 1 447 1308 1758 2100 3894 4369 4454 4683
Size: 446 861 450 342 1794 475 85 229
Cuts at: 4683 5150 5173 5508 5754 6060 6072 6158 7210
Size: 467 23 335 246 306 12 86 1052
Cuts at: 7210 1
Size: 449
Fragments arranged by size:
1794 1052 861 475 467 450 449 446
342 335 306 246 229 86 85 23
12
BstZ17I GTA'TAC
Cuts at: 1514 1514
Size: 7658
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5608 5789 5952 5608
Size: 181 163 7314
Fragments arranged by size:
7314 181 163
BtsI GCAGTG_nn'
Cuts at: 4212 4901 4212
Size: 689 6969
DraI TTT'AAA
Cuts at: 356 4134 4433 4657 356
Size: 3778 299 224 3357
Fragments arranged by size:
3778 3357 299 224
DraIII CAC_nnn'GTG
Cuts at: 841 841
Size: 7658
DrdI GACnn_nn'nnGTC
Cuts at: 1811 5364 6708 1811
Size: 3553 1344 2761
Fragments arranged by size:
3553 2761 1344
EaeI y'GGCC_r
Cuts at: 5243 5417 5808 5835 7180 5243
Size: 174 391 27 1345 5721
Fragments arranged by size:
5721 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5243 5243
Size: 7658
EarI CTCTTCn'nnn_
Cuts at: 338 893 1274 2885 3365 3821 5681 5891 338
Size: 555 381 1611 480 456 1860 210 2105
Fragments arranged by size:
2105 1860 1611 555 480 456 381 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5866 6608 6754 7468 5866
Size: 742 146 714 6056
Fragments arranged by size:
6056 742 714 146
EcoICRI GAG'CTC
Cuts at: 4670 4670
Size: 7658
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 949 1382 1384 1460 1485 1853 3322 3712
Size: 815 433 2 76 25 368 1469 390
Cuts at: 3712 3766 3860 5483 5915 6268 6930 134
Size: 54 94 1623 432 353 662 862
Fragments arranged by size:
1623 1469 862 815 662 433 432 390
368 353 94 76 54 25 2
EcoO109I rG'GnC_Cy
Cuts at: 2137 2494 4797 2137
Size: 357 2303 4998
Fragments arranged by size:
4998 2303 357
EcoRI G'AATT_C
Cuts at: 133 224 1388 4662 133
Size: 91 1164 3274 3129
Fragments arranged by size:
3274 3129 1164 91
EcoRV GAT'ATC
Cuts at: 1965 1965
Size: 7658
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 433 546 970 2795 3200 3502 3674 3742 4354
Size: 113 424 1825 405 302 172 68 612
Cuts at: 4354 5677 433
Size: 1323 2414
Fragments arranged by size:
2414 1825 1323 612 424 405 302 172
113 68
FspI TGC'GCA
Cuts at: 1031 5439 1031
Size: 4408 3250
HaeII r_GCGC'y
Cuts at: 5092 5340 6570 7341 5092
Size: 248 1230 771 5409
Fragments arranged by size:
5409 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 708 740 2638 2670 3996 4028 4035 4067 4429
Size: 32 1898 32 1326 32 7 32 362
Cuts at: 4429 4461 5500 5530 5532 5562 708
Size: 32 1039 30 2 30 2804
Fragments arranged by size:
2804 1898 1326 1039 362 32 32 32
32 32 30 30 7 2
HincII GTy'rAC
Cuts at: 4697 4697
Size: 7658
HindIII A'AGCT_T
Cuts at: 648 1594 2252 4071 4318 648
Size: 946 658 1819 247 3988
Fragments arranged by size:
3988 1819 946 658 247
KasI G'GCGC_C
Cuts at: 5088 5336 5088
Size: 248 7410
KpnI G_GTAC'C
Cuts at: 4678 4678
Size: 7658
MfeI C'AATT_G
Cuts at: 2075 2075
Size: 7658
MluI A'CGCG_T
Cuts at: 7091 7091
Size: 7658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 6417 6601 6417
Size: 184 7474
MscI TGG'CCA
Cuts at: 5419 5419
Size: 7658
MslI CAynn'nnrTG
Cuts at: 557 563 2702 3127 4414 5647 5774 557
Size: 6 2139 425 1287 1233 127 2441
Fragments arranged by size:
2441 2139 1287 1233 425 127 6
MspA1I CmG'CkG
Cuts at: 4272 4555 4769 5083 5443 6227 6472 4272
Size: 283 214 314 360 784 245 5458
Fragments arranged by size:
5458 784 360 314 283 245 214
NaeI GCC'GGC
Cuts at: 5839 5839
Size: 7658
NarI GG'CG_CC
Cuts at: 5089 5337 5089
Size: 248 7410
NdeI CA'TA_TG
Cuts at: 2391 2391
Size: 7658
NgoMIV G'CCGG_C
Cuts at: 5837 5837
Size: 7658
NheI G'CTAG_C
Cuts at: 855 1825 855
Size: 970 6688
NsiI A_TGCA'T
Cuts at: 618 4542 618
Size: 3924 3734
NspI r_CATG'y
Cuts at: 259 3272 3540 4280 4711 5211 6814 259
Size: 3013 268 740 431 500 1603 1103
Fragments arranged by size:
3013 1603 1103 740 500 431 268
PciI A'CATG_T
Cuts at: 3268 4276 6810 3268
Size: 1008 2534 4116
Fragments arranged by size:
4116 2534 1008
PflMI CCAn_nnn'nTGG
Cuts at: 1508 1508
Size: 7658
PmeI GTTT'AAAC
Cuts at: 4657 4657
Size: 7658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6104 6136 6104
Size: 32 7626
PpuMI rG'GwC_Cy
Cuts at: 2137 2494 2137
Size: 357 7301
PsiI TTA'TAA
Cuts at: 1015 1081 2546 1015
Size: 66 1465 6127
Fragments arranged by size:
6127 1465 66
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3734 3766 3734
Size: 32 7626
PstI C_TGCA'G
Cuts at: 1264 4705 5390 1264
Size: 3441 685 3532
Fragments arranged by size:
3532 3441 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7658
PvuII CAG'CTG
Cuts at: 4272 4555 5083 5443 4272
Size: 283 528 360 6487
Fragments arranged by size:
6487 528 360 283
RsrII CG'GwC_CG
Cuts at: 5853 5853
Size: 7658
SacI G_AGCT'C
Cuts at: 4672 4672
Size: 7658
SalI G'TCGA_C
Cuts at: 4695 4695
Size: 7658
SapI GCTCTTCn'nnn_
Cuts at: 3365 5681 5891 3365
Size: 2316 210 5132
Fragments arranged by size:
5132 2316 210
SbfI CC_TGCA'GG
Cuts at: 4705 4705
Size: 7658
ScaI AGT'ACT
Cuts at: 3198 3657 7175 3198
Size: 459 3518 3681
Fragments arranged by size:
3681 3518 459
SfcI C'TryA_G
Cuts at: 33 1225 1260 1451 2228 2828 2987 3146 3282
Size: 1192 35 191 777 600 159 159 136
Cuts at: 3282 4375 4701 5386 6354 6545 33
Size: 1093 326 685 968 191 1146
Fragments arranged by size:
1192 1146 1093 968 777 685 600 326
191 191 159 159 136 35
SfoI GGC'GCC
Cuts at: 5090 5338 5090
Size: 248 7410
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7658
SmaI CCC'GGG
Cuts at: 4680 4680
Size: 7658
SmlI C'TyrA_G
Cuts at: 418 4813 5728 6442 6704 418
Size: 4395 915 714 262 1372
Fragments arranged by size:
4395 1372 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7658
SphI G_CATG'C
Cuts at: 259 4711 5211 259
Size: 4452 500 2706
Fragments arranged by size:
4452 2706 500
SspI AAT'ATT
Cuts at: 1106 3610 4138 4607 4645 1106
Size: 2504 528 469 38 4119
Fragments arranged by size:
4119 2504 528 469 38
StuI AGG'CCT
Cuts at: 548 548
Size: 7658
StyI C'CwwG_G
Cuts at: 443 2140 4126 4185 4792 5146 443
Size: 1697 1986 59 607 354 2955
Fragments arranged by size:
2955 1986 1697 607 354 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 5241 5374 5833 6042 5241
Size: 133 459 209 6857
Fragments arranged by size:
6857 459 209 133
TatI w'GTAC_w
Cuts at: 332 1690 2575 2584 3196 3550 3655 3773 3849
Size: 1358 885 9 612 354 105 118 76
Cuts at: 3849 7173 332
Size: 3324 817
Fragments arranged by size:
3324 1358 885 817 612 354 118 105
76 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2932 2964 7175 7207 2932
Size: 32 4211 32 3383
Fragments arranged by size:
4211 3383 32 32
Tth111I GACn'n_nGTC
Cuts at: 5455 5455
Size: 7658
XbaI T'CTAG_A
Cuts at: 2401 4689 2401
Size: 2288 5370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2934 5153 2934
Size: 2219 5439
XmaI C'CCGG_G
Cuts at: 4678 4678
Size: 7658
XmnI GAAnn'nnTTC
Cuts at: 902 1883 902
Size: 981 6677
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI
NsiI NspI PciI PflMI PmeI PpiI PpuMI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
ApaI AscI AvrII BaeI BglI BmgBI BsaXI BsiWI
BsmBI BspEI BssHII BstEII Bsu36I BtgI ClaI CspCI
EcoNI FseI FspAI HpaI NcoI NotI NruI PacI
PasI PfoI PmlI PshAI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI