Restriction map for
pF1KA1419
(Circular) (Six-base) MAPSORT of: pF1KA1419.seq Check: 8990 from: 1 to: 10409
pF1KA1419 10409 bp
With 182 enzymes: SgfI *
May 27, 2009 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 279 780 1098 1345 2991 279
Size: 501 318 247 1646 7697
Fragments arranged by size:
7697 1646 501 318 247
AatII G_ACGT'C
Cuts at: 2636 6950 2636
Size: 4314 6095
AccI GT'mk_AC
Cuts at: 4591 6146 7447 4591
Size: 1555 1301 7553
Fragments arranged by size:
7553 1555 1301
Acc65I G'GTAC_C
Cuts at: 7425 7425
Size: 10409
AclI AA'CG_TT
Cuts at: 10249 10249
Size: 10409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 645 1292 2177 3254 3310 3655 4648 5827
Size: 401 647 885 1077 56 345 993 1179
Cuts at: 5827 6741 7286 8234 8666 9413 9681 244
Size: 914 545 948 432 747 268 972
Fragments arranged by size:
1179 1077 993 972 948 914 885 747
647 545 432 401 345 268 56
AfeI AGC'GCT
Cuts at: 3373 6364 3373
Size: 2991 7418
AflIII A'CryG_T
Cuts at: 5933 7761 8865 9842 5933
Size: 1828 1104 977 6500
Fragments arranged by size:
6500 1828 1104 977
AgeI A'CCGG_T
Cuts at: 8797 8797
Size: 10409
AhdI GACnn_n'nnGTC
Cuts at: 4128 6522 4128
Size: 2394 8015
AleI CACnn'nnGTG
Cuts at: 1459 1459
Size: 10409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1178 3035 3268 4640 6553 7264 1178
Size: 1857 233 1372 1913 711 4323
Fragments arranged by size:
4323 1913 1857 1372 711 233
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 346 378 346
Size: 32 10377
AlwNI CAG_nnn'CTG
Cuts at: 294 303 606 3304 5151 5831 6289 6549 7714
Size: 9 303 2698 1847 680 458 260 1165
Cuts at: 7714 9281 294
Size: 1567 1422
Fragments arranged by size:
2698 1847 1567 1422 1165 680 458 303
260 9
ApaI G_GGCC'C
Cuts at: 208 756 1468 208
Size: 548 712 9149
Fragments arranged by size:
9149 712 548
ApaLI G'TGCA_C
Cuts at: 160 9179 160
Size: 9019 1390
ApoI r'AATT_y
Cuts at: 570 2313 3048 4068 5595 6171 7413 9762 10018
Size: 1743 735 1020 1527 576 1242 2349 256
Cuts at: 10018 570
Size: 961
Fragments arranged by size:
2349 1743 1527 1242 1020 961 735 576
256
AscI GG'CGCG_CC
Cuts at: 7089 7089
Size: 10409
AseI AT'TA_AT
Cuts at: 20 5249 20
Size: 5229 5180
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10409
AvaI C'yCGr_G
Cuts at: 197 1373 1696 1825 2525 2606 5871 6999 7223
Size: 1176 323 129 700 81 3265 1128 224
Cuts at: 7223 7429 197
Size: 206 3177
Fragments arranged by size:
3265 3177 1176 1128 700 323 224 206
129 81
AvrII C'CTAG_G
Cuts at: 3819 3819
Size: 10409
BamHI G'GATC_C
Cuts at: 4945 7104 7434 4945
Size: 2159 330 7920
Fragments arranged by size:
7920 2159 330
BanI G'GyrC_C
Cuts at: 775 792 4261 7425 7839 8087 8122 775
Size: 17 3469 3164 414 248 35 3062
Fragments arranged by size:
3469 3164 3062 414 248 35 17
BanII G_rGCy'C
Cuts at: 208 329 756 1348 1468 1775 1894 2207 3624
Size: 121 427 592 120 307 119 313 1417
Cuts at: 3624 5276 5844 6302 6640 7423 8453 208
Size: 1652 568 458 338 783 1030 2164
Fragments arranged by size:
2164 1652 1417 1030 783 592 568 458
427 338 313 307 121 120 119
BbeI G_GCGC'C
Cuts at: 7843 8091 7843
Size: 248 10161
BbsI GAAGACnn'nnnn_
Cuts at: 515 1679 3298 4465 515
Size: 1164 1619 1167 6459
Fragments arranged by size:
6459 1619 1167 1164
BbvCI CC'TCA_GC
Cuts at: 782 1195 2081 2929 4360 4798 5590 782
Size: 413 886 848 1431 438 792 5601
Fragments arranged by size:
5601 1431 886 848 792 438 413
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 10375
BciVI GTATCCnnnnn_n'
Cuts at: 747 912 1230 2528 2746 8307 9068 10409 747
Size: 165 318 1298 218 5561 761 1341 747
Fragments arranged by size:
5561 1341 1298 761 747 318 218 165
BclI T'GATC_A
Cuts at: 3509 7929 3509
Size: 4420 5989
BfrBI ATG'CAT
Cuts at: 5627 5627
Size: 10409
BglI GCCn_nnn'nGGC
Cuts at: 112 773 1245 3502 4234 4910 6416 112
Size: 661 472 2257 732 676 1506 4105
Fragments arranged by size:
4105 2257 1506 732 676 661 472
BglII A'GATC_T
Cuts at: 374 6650 7924 9961 374
Size: 6276 1274 2037 822
Fragments arranged by size:
6276 2037 1274 822
BlpI GC'TnA_GC
Cuts at: 1888 4224 5165 7521 1888
Size: 2336 941 2356 4776
Fragments arranged by size:
4776 2356 2336 941
Bme1580I G_kGCm'C
Cuts at: 164 191 208 756 1468 1530 1697 1845 3791
Size: 27 17 548 712 62 167 148 1946
Cuts at: 3791 4043 4264 8034 8127 9183 164
Size: 252 221 3770 93 1056 1390
Fragments arranged by size:
3770 1946 1390 1056 712 548 252 221
167 148 93 62 27 17
BmgBI CAC'GTC
Cuts at: 3176 3176
Size: 10409
BmrI ACTGGGnnnn_n'
Cuts at: 396 756 1673 2261 2470 3562 3689 5295 5439
Size: 360 917 588 209 1092 127 1606 144
Cuts at: 5439 6145 6431 7250 7621 7702 8037 10181 396
Size: 706 286 819 371 81 335 2144 624
Fragments arranged by size:
2144 1606 1092 917 819 706 624 588
371 360 335 286 209 144 127 81
BmtI G_CTAG'C
Cuts at: 4210 4502 4210
Size: 292 10117
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2199 3048 5421 5615 7087 8776 2199
Size: 849 2373 194 1472 1689 3832
Fragments arranged by size:
3832 2373 1689 1472 849 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 1417 1453 1920 2475 3946 3989 4197 4686
Size: 993 36 467 555 1471 43 208 489
Cuts at: 4686 5128 5334 5361 6946 7057 7305 424
Size: 442 206 27 1585 111 248 3528
Fragments arranged by size:
3528 1585 1471 993 555 489 467 442
248 208 206 111 43 36 27
Bpu10I CC'TnA_GC
Cuts at: 584 782 1195 1893 2081 2929 3236 3652 4147
Size: 198 413 698 188 848 307 416 495
Cuts at: 4147 4360 4798 5270 5590 584
Size: 213 438 472 320 5403
Fragments arranged by size:
5403 848 698 495 472 438 416 413
320 307 213 198 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 1480 1639 5804 7585 8464 8956 9254 622
Size: 858 159 4165 1781 879 492 298 1777
Fragments arranged by size:
4165 1781 1777 879 858 492 298 159
BsaI GGTCTCn'nnnn_
Cuts at: 2147 2824 5638 10113 2147
Size: 677 2814 4475 2443
Fragments arranged by size:
4475 2814 2443 677
BsaAI yAC'GTr
Cuts at: 186 6633 7762 186
Size: 6447 1129 2833
Fragments arranged by size:
6447 2833 1129
BsaBI GATnn'nnATC
Cuts at: 6 7923 9960 6
Size: 7917 2037 455
Fragments arranged by size:
7917 2037 455
BsaHI Gr'CG_yC
Cuts at: 461 1957 2495 2633 2684 4142 6947 7840 8088
Size: 1496 538 138 51 1458 2805 893 248
Cuts at: 8088 8790 10286 461
Size: 702 1496 584
Fragments arranged by size:
2805 1496 1496 1458 893 702 584 538
248 138 51
BsaWI w'CCGG_w
Cuts at: 2729 3677 4720 4859 6234 7598 8119 8797 9071
Size: 948 1043 139 1375 1364 521 678 274
Cuts at: 9071 9218 9629 2729
Size: 147 411 3509
Fragments arranged by size:
3509 1375 1364 1043 948 678 521 411
274 147 139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1676 1706 1863 1893 2875 2905 2938 2968 3387
Size: 30 157 30 982 30 33 30 419
Cuts at: 3387 3417 4108 4138 4149 4179 4798 4828 5332
Size: 30 691 30 11 30 619 30 504
Cuts at: 5332 5362 7198 7228 7330 7360 1676
Size: 30 1836 30 102 30 4725
Fragments arranged by size:
4725 1836 982 691 619 504 419 157
102 33 30 30 30 30 30 30
30 30 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 705 1050 1131 1441 1675 2946 2979 4269 4401
Size: 345 81 310 234 1271 33 1290 132
Cuts at: 4401 5325 5530 6834 7374 705
Size: 924 205 1304 540 3740
Fragments arranged by size:
3740 1304 1290 1271 924 540 345 310
234 205 132 81 33
BseYI C'CCAG_C
Cuts at: 209 1408 1845 1904 1916 2087 2206 2876 3427
Size: 1199 437 59 12 171 119 670 551
Cuts at: 3427 3721 3929 4219 4378 4604 4742 5075 5894
Size: 294 208 290 159 226 138 333 819
Cuts at: 5894 6412 7834 8592 9169 9665 209
Size: 518 1422 758 577 496 953
Fragments arranged by size:
1422 1199 953 819 758 670 577 551
518 496 437 333 294 290 226 208
171 159 138 119 59 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1184 3031 3560 166
Size: 1018 1847 529 7015
Fragments arranged by size:
7015 1847 1018 529
BsiEI CG_ry'CG
Cuts at: 104 7951 7997 9205 104
Size: 7847 46 1208 1308
Fragments arranged by size:
7847 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 164 1894 5276 6101 6389 6640 7423 8201 8391
Size: 1730 3382 825 288 251 783 778 190
Cuts at: 8391 9183 164
Size: 792 1390
Fragments arranged by size:
3382 1730 1390 825 792 783 778 288
251 190
BsmI GAATG_Cn'
Cuts at: 5629 5962 5629
Size: 333 10076
BsmBI CGTCTCn'nnnn_
Cuts at: 430 2486 2640 2858 5352 6375 430
Size: 2056 154 218 2494 1023 4464
Fragments arranged by size:
4464 2494 2056 1023 218 154
Bsp1286I G_dGCh'C
Cuts at: 164 191 208 329 756 1348 1468 1530 1697
Size: 27 17 121 427 592 120 62 167
Cuts at: 1697 1775 1845 1894 2207 3624 3791 4043 4264
Size: 78 70 49 313 1417 167 252 221
Cuts at: 4264 5276 5844 6101 6302 6389 6640 7423 8034
Size: 1012 568 257 201 87 251 783 611
Cuts at: 8034 8127 8201 8391 8453 9183 164
Size: 93 74 190 62 730 1390
Fragments arranged by size:
1417 1390 1012 783 730 611 592 568
427 313 257 252 251 221 201 190
167 167 121 120 93 87 78 74
70 62 62 49 27 17
BspEI T'CCGG_A
Cuts at: 2729 4720 4859 9629 2729
Size: 1991 139 4770 3509
Fragments arranged by size:
4770 3509 1991 139
BspHI T'CATG_A
Cuts at: 9585 9585
Size: 10409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 279 780 1098 1345 2531 2991 3530 6746 7459
Size: 501 318 247 1186 460 539 3216 713
Cuts at: 7459 7673 7975 8356 279
Size: 214 302 381 2332
Fragments arranged by size:
3216 2332 1186 713 539 501 460 381
318 302 247 214
BsrBI CCG'CTC
Cuts at: 50 344 1106 1392 1869 8701 8755 10239 10404
Size: 294 762 286 477 6832 54 1484 165
Cuts at: 10404 50
Size: 55
Fragments arranged by size:
6832 1484 762 477 294 286 165 55
54
BsrDI GCAATG_nn'
Cuts at: 1502 1566 2517 8321 1502
Size: 64 951 5804 3590
Fragments arranged by size:
5804 3590 951 64
BsrFI r'CCGG_y
Cuts at: 111 1284 2798 3496 6443 8407 8588 8797 111
Size: 1173 1514 698 2947 1964 181 209 1723
Fragments arranged by size:
2947 1964 1723 1514 1173 698 209 181
BsrGI T'GTAC_A
Cuts at: 2135 2135
Size: 10409
BssHII G'CGCG_C
Cuts at: 7089 7089
Size: 10409
BssSI C'ACGA_G
Cuts at: 1030 7159 8680 9038 1030
Size: 6129 1521 358 2401
Fragments arranged by size:
6129 2401 1521 358
BstAPI GCAn_nnn'nTGC
Cuts at: 294 303 792 5831 7786 294
Size: 9 489 5039 1955 2917
Fragments arranged by size:
5039 2917 1955 489 9
BstBI TT'CG_AA
Cuts at: 1415 8770 1415
Size: 7355 3054
BstEII G'GTnAC_C
Cuts at: 4114 7270 4114
Size: 3156 7253
BstXI CCAn_nnnn'nTGG
Cuts at: 4878 6990 7081 4878
Size: 2112 91 8206
Fragments arranged by size:
8206 2112 91
BstYI r'GATC_y
Cuts at: 1 272 374 435 535 2259 4945 6650 7104
Size: 271 102 61 100 1724 2686 1705 454
Cuts at: 7104 7434 7901 7924 8259 8505 9517 9603 9615
Size: 330 467 23 335 246 1012 86 12
Cuts at: 9615 9961 1
Size: 346 449
Fragments arranged by size:
2686 1724 1705 1012 467 454 449 346
335 330 271 246 102 100 86 61
23 12
BstZ17I GTA'TAC
Cuts at: 4592 4592
Size: 10409
Bsu36I CC'TnA_GG
Cuts at: 1250 2264 6817 7190 1250
Size: 1014 4553 373 4469
Fragments arranged by size:
4553 4469 1014 373
BtgI C'CryG_G
Cuts at: 1214 1460 1775 2117 2396 5882 7135 1214
Size: 246 315 342 279 3486 1253 4488
Fragments arranged by size:
4488 3486 1253 342 315 279 246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2280 2717 4029 4389 8359 8540 8703 2280
Size: 437 1312 360 3970 181 163 3986
Fragments arranged by size:
3986 3970 1312 437 360 181 163
BtsI GCAGTG_nn'
Cuts at: 2111 3405 4143 4158 4371 5294 5584 5832 7652
Size: 1294 738 15 213 923 290 248 1820
Cuts at: 7652 2111
Size: 4868
Fragments arranged by size:
4868 1820 1294 923 738 290 248 213
15
ClaI AT'CG_AT
Cuts at: 6986 6986
Size: 10409
DraI TTT'AAA
Cuts at: 7408 7408
Size: 10409
DraIII CAC_nnn'GTG
Cuts at: 5100 6633 5100
Size: 1533 8876
DrdI GACnn_nn'nnGTC
Cuts at: 884 2389 4467 8115 8973 884
Size: 1505 2078 3648 858 2320
Fragments arranged by size:
3648 2320 2078 1505 858
EaeI y'GGCC_r
Cuts at: 113 414 746 1145 2409 3503 6437 6807 7994
Size: 301 332 399 1264 1094 2934 370 1187
Cuts at: 7994 8168 8559 8586 9931 113
Size: 174 391 27 1345 591
Fragments arranged by size:
2934 1345 1264 1187 1094 591 399 391
370 332 301 174 27
EagI C'GGCC_G
Cuts at: 7994 7994
Size: 10409
EarI CTCTTCn'nnn_
Cuts at: 334 611 661 1412 1537 1810 1950 4491 4882
Size: 277 50 751 125 273 140 2541 391
Cuts at: 4882 6729 6973 8432 8642 334
Size: 1847 244 1459 210 2101
Fragments arranged by size:
2541 2101 1847 1459 751 391 277 273
244 210 140 125 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2568 3150 3480 8617 8927 9073 10219 2568
Size: 582 330 5137 310 146 1146 2758
Fragments arranged by size:
5137 2758 1146 582 330 310 146
EcoICRI GAG'CTC
Cuts at: 1892 5274 6638 7421 1892
Size: 3382 1364 783 4880
Fragments arranged by size:
4880 3382 1364 783
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 424 645 1292 1417 1453 1920 2177 2475
Size: 180 221 647 125 36 467 257 298
Cuts at: 2475 3254 3310 3655 3946 3989 4197 4648 4686
Size: 779 56 345 291 43 208 451 38
Cuts at: 4686 5128 5334 5361 5827 6741 6946 7057 7286
Size: 442 206 27 466 914 205 111 229
Cuts at: 7286 7305 8234 8666 9413 9681 244
Size: 19 929 432 747 268 972
Fragments arranged by size:
972 929 914 779 747 647 467 466
451 442 432 345 298 291 268 257
229 221 208 206 205 180 125 111
56 43 38 36 27 19
EcoNI CCTnn'n_nnAGG
Cuts at: 1657 4577 5871 1657
Size: 2920 1294 6195
Fragments arranged by size:
6195 2920 1294
EcoO109I rG'GnC_Cy
Cuts at: 205 752 753 886 1254 1370 1465 1614 4374
Size: 547 1 133 368 116 95 149 2760
Cuts at: 4374 7548 205
Size: 3174 3066
Fragments arranged by size:
3174 3066 2760 547 368 149 133 116
95 1
EcoRI G'AATT_C
Cuts at: 3048 5595 6171 7413 3048
Size: 2547 576 1242 6044
Fragments arranged by size:
6044 2547 1242 576
EcoRV GAT'ATC
Cuts at: 5700 5700
Size: 10409
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3361 8428 3361
Size: 5067 5342
FspI TGC'GCA
Cuts at: 6859 8190 6859
Size: 1331 9078
HaeII r_GCGC'y
Cuts at: 170 1852 3375 6366 7843 8091 9113 10092 170
Size: 1682 1523 2991 1477 248 1022 979 487
Fragments arranged by size:
2991 1682 1523 1477 1022 979 487 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 246 278 2485 2517 2762 2794 2875 2907 3076
Size: 32 2207 32 245 32 81 32 169
Cuts at: 3076 3108 3123 3155 3976 4008 4114 4146 4149
Size: 32 15 32 821 32 106 32 3
Cuts at: 4149 4181 4937 4969 5332 5364 6032 6064 6508
Size: 32 756 32 363 32 668 32 444
Cuts at: 6508 6540 7061 7093 7198 7230 7330 7362 8251
Size: 32 521 32 105 32 100 32 889
Cuts at: 8251 8281 8283 8313 246
Size: 30 2 30 2342
Fragments arranged by size:
2342 2207 889 821 756 668 521 444
363 245 169 106 105 100 81 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 30
30 15 3 2
HincII GTy'rAC
Cuts at: 941 3846 5921 6147 6485 7448 941
Size: 2905 2075 226 338 963 3902
Fragments arranged by size:
3902 2905 2075 963 338 226
HindIII A'AGCT_T
Cuts at: 5462 5462
Size: 10409
KasI G'GCGC_C
Cuts at: 7839 8087 7839
Size: 248 10161
KpnI G_GTAC'C
Cuts at: 7429 7429
Size: 10409
MluI A'CGCG_T
Cuts at: 9842 9842
Size: 10409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3188 3580 3728 3779 4268 4808 5630 6243 6373
Size: 392 148 51 489 540 822 613 130
Cuts at: 6373 6578 7373 9080 9264 3188
Size: 205 795 1707 184 4333
Fragments arranged by size:
4333 1707 822 795 613 540 489 392
205 184 148 130 51
MscI TGG'CCA
Cuts at: 3505 6439 8170 3505
Size: 2934 1731 5744
Fragments arranged by size:
5744 2934 1731
MslI CAynn'nnrTG
Cuts at: 1459 2401 2677 3109 3250 4771 6283 6988 7134
Size: 942 276 432 141 1521 1512 705 146
Cuts at: 7134 7291 7367 8398 8525 1459
Size: 157 76 1031 127 3343
Fragments arranged by size:
3343 1521 1512 1031 942 705 432 276
157 146 141 127 76
MspA1I CmG'CkG
Cuts at: 316 333 907 1587 1690 1920 2598 2876 2903
Size: 17 574 680 103 230 678 278 27
Cuts at: 2903 3101 3521 4054 4337 4675 5079 5164 5233
Size: 198 420 533 283 338 404 85 69
Cuts at: 5233 5733 6416 7278 7520 7834 8194 9207 9452
Size: 500 683 862 242 314 360 1013 245
Cuts at: 9452 316
Size: 1273
Fragments arranged by size:
1273 1013 862 683 680 678 574 533
500 420 404 360 338 314 283 278
245 242 230 198 103 85 69 27
17
NaeI GCC'GGC
Cuts at: 113 1286 3498 8590 113
Size: 1173 2212 5092 1932
Fragments arranged by size:
5092 2212 1932 1173
NarI GG'CG_CC
Cuts at: 7840 8088 7840
Size: 248 10161
NcoI C'CATG_G
Cuts at: 1775 5882 7135 1775
Size: 4107 1253 5049
Fragments arranged by size:
5049 4107 1253
NgoMIV G'CCGG_C
Cuts at: 111 1284 3496 8588 111
Size: 1173 2212 5092 1932
Fragments arranged by size:
5092 2212 1932 1173
NheI G'CTAG_C
Cuts at: 4206 4498 4206
Size: 292 10117
NsiI A_TGCA'T
Cuts at: 5629 5629
Size: 10409
NspI r_CATG'y
Cuts at: 1452 1824 5937 5989 6600 7290 7462 7962 8869
Size: 372 4113 52 611 690 172 500 907
Cuts at: 8869 1452
Size: 2992
Fragments arranged by size:
4113 2992 907 690 611 500 372 172
52
PasI CC'CwG_GG
Cuts at: 2853 7097 2853
Size: 4244 6165
PciI A'CATG_T
Cuts at: 5933 8865 5933
Size: 2932 7477
PflMI CCAn_nnn'nTGG
Cuts at: 489 1178 3304 5733 6902 489
Size: 689 2126 2429 1169 3996
Fragments arranged by size:
3996 2429 2126 1169 689
PfoI T'CCnGG_A
Cuts at: 2452 3658 4919 5744 6614 6923 7049 2452
Size: 1206 1261 825 870 309 126 5812
Fragments arranged by size:
5812 1261 1206 870 825 309 126
PmeI GTTT'AAAC
Cuts at: 7408 7408
Size: 10409
PmlI CAC'GTG
Cuts at: 186 6633 186
Size: 6447 3962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9548 9580 9548
Size: 32 10377
PpuMI rG'GwC_Cy
Cuts at: 886 1370 1614 4374 886
Size: 484 244 2760 6921
Fragments arranged by size:
6921 2760 484 244
PspOMI G'GGCC_C
Cuts at: 204 752 1464 204
Size: 548 712 9149
Fragments arranged by size:
9149 712 548
PspXI vC'TCGA_Gb
Cuts at: 2525 2525
Size: 10409
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2337 2369 2337
Size: 32 10377
PstI C_TGCA'G
Cuts at: 299 308 615 912 4516 4656 5310 5836 6362
Size: 9 307 297 3604 140 654 526 526
Cuts at: 6362 6743 7181 7304 7456 8141 299
Size: 381 438 123 152 685 2567
Fragments arranged by size:
3604 2567 685 654 526 526 438 381
307 297 152 140 123 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10409
PvuII CAG'CTG
Cuts at: 1690 1920 2598 2876 4054 4337 5079 5164 5233
Size: 230 678 278 1178 283 742 85 69
Cuts at: 5233 5733 7834 8194 1690
Size: 500 2101 360 3905
Fragments arranged by size:
3905 2101 1178 742 678 500 360 283
278 230 85 69
RsrII CG'GwC_CG
Cuts at: 2732 8604 2732
Size: 5872 4537
SacI G_AGCT'C
Cuts at: 1894 5276 6640 7423 1894
Size: 3382 1364 783 4880
Fragments arranged by size:
4880 3382 1364 783
SalI G'TCGA_C
Cuts at: 6145 7446 6145
Size: 1301 9108
SanDI GG'GwC_CC
Cuts at: 1370 4374 1370
Size: 3004 7405
SapI GCTCTTCn'nnn_
Cuts at: 334 1810 4882 6973 8432 8642 334
Size: 1476 3072 2091 1459 210 2101
Fragments arranged by size:
3072 2101 2091 1476 1459 210
SbfI CC_TGCA'GG
Cuts at: 7456 7456
Size: 10409
ScaI AGT'ACT
Cuts at: 4521 9926 4521
Size: 5405 5004
SexAI A'CCwGG_T
Cuts at: 3834 3834
Size: 10409
SfcI C'TryA_G
Cuts at: 33 295 304 611 908 4173 4512 4652 5306
Size: 262 9 307 297 3265 339 140 654
Cuts at: 5306 5832 6192 6358 6739 7177 7300 7452 8137
Size: 526 360 166 381 438 123 152 685
Cuts at: 8137 9130 9321 33
Size: 993 191 1121
Fragments arranged by size:
3265 1121 993 685 654 526 438 381
360 339 307 297 262 191 166 152
140 123 9
SfoI GGC'GCC
Cuts at: 7841 8089 7841
Size: 248 10161
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10409
SmaI CCC'GGG
Cuts at: 199 1375 1827 2608 7431 199
Size: 1176 452 781 4823 3177
Fragments arranged by size:
4823 3177 1176 781 452
SmlI C'TyrA_G
Cuts at: 637 1495 1654 2525 5819 6999 7564 8479 8971
Size: 858 159 871 3294 1180 565 915 492
Cuts at: 8971 9233 637
Size: 262 1813
Fragments arranged by size:
3294 1813 1180 915 871 858 565 492
262 159
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10409
SphI G_CATG'C
Cuts at: 1452 1824 7462 7962 1452
Size: 372 5638 500 3899
Fragments arranged by size:
5638 3899 500 372
SrfI GCCC'GGGC
Cuts at: 199 199
Size: 10409
StuI AGG'CCT
Cuts at: 653 3779 4661 653
Size: 3126 882 6401
Fragments arranged by size:
6401 3126 882
StyI C'CwwG_G
Cuts at: 1097 1177 1775 1856 3256 3819 4415 4958 5882
Size: 80 598 81 1400 563 596 543 924
Cuts at: 5882 6804 6896 7135 7543 7897 1097
Size: 922 92 239 408 354 3609
Fragments arranged by size:
3609 1400 924 922 598 596 563 543
408 354 239 92 81 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1221 2056 2749 3578 4783 5089 6753 7107 7992
Size: 835 693 829 1205 306 1664 354 885
Cuts at: 7992 8125 8584 8793 1221
Size: 133 459 209 2837
Fragments arranged by size:
2837 1664 1205 885 835 829 693 459
354 306 209 133
TatI w'GTAC_w
Cuts at: 2135 2573 4519 9924 2135
Size: 438 1946 5405 2620
Fragments arranged by size:
5405 2620 1946 438
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 224 256 1676 1708 1863 1895 1924 1956 3387
Size: 32 1420 32 155 32 29 32 1431
Cuts at: 3387 3419 4375 4407 4798 4830 5551 5583 5754
Size: 32 956 32 391 32 721 32 171
Cuts at: 5754 5786 6007 6039 9926 9958 224
Size: 32 221 32 3887 32 675
Fragments arranged by size:
3887 1431 1420 956 721 675 391 221
171 155 32 32 32 32 32 32
32 32 32 32 32 29
Tth111I GACn'n_nGTC
Cuts at: 5344 8206 5344
Size: 2862 7547
XbaI T'CTAG_A
Cuts at: 7440 7440
Size: 10409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 769 2020 3125 6510 7119 7904 769
Size: 1251 1105 3385 609 785 3274
Fragments arranged by size:
3385 3274 1251 1105 785 609
XhoI C'TCGA_G
Cuts at: 2525 6999 2525
Size: 4474 5935
XmaI C'CCGG_G
Cuts at: 197 1373 1825 2606 7429 197
Size: 1176 452 781 4823 3177
Fragments arranged by size:
4823 3177 1176 781 452
XmnI GAAnn'nnTTC
Cuts at: 1327 3345 4937 6799 1327
Size: 2018 1592 1862 4937
Fragments arranged by size:
4937 2018 1862 1592
ZraI GAC'GTC
Cuts at: 2634 6948 2634
Size: 4314 6095
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII BaeI BsiWI CspCI FseI FspAI HpaI MfeI
NdeI NotI NruI PacI PshAI PsiI SacII SfiI
SgrAI SnaBI SspI SwaI