Restriction map for
pF1KA1426
(Circular) (Six-base) MAPSORT of: pF1KA1426.seq Check: 1359 from: 1 to: 6308
pF1KA1426 6308 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1410 2150 1410
Size: 740 5568
AatII G_ACGT'C
Cuts at: 2711 2711
Size: 6308
AccI GT'mk_AC
Cuts at: 1568 3346 1568
Size: 1778 4530
Acc65I G'GTAC_C
Cuts at: 2951 3324 2951
Size: 373 5935
AclI AA'CG_TT
Cuts at: 2207 6148 2207
Size: 3941 2367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 406 1717 2435 4133 4565 5312 5580 226
Size: 180 1311 718 1698 432 747 268 954
Fragments arranged by size:
1698 1311 954 747 718 432 268 180
AflIII A'CryG_T
Cuts at: 3660 4764 5741 3660
Size: 1104 977 4227
Fragments arranged by size:
4227 1104 977
AgeI A'CCGG_T
Cuts at: 4696 4696
Size: 6308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 527 3082 527
Size: 2555 3753
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1840 1872 1840
Size: 32 6276
AlwNI CAG_nnn'CTG
Cuts at: 225 837 2076 3613 5180 225
Size: 612 1239 1537 1567 1353
Fragments arranged by size:
1567 1537 1353 1239 612
ApaI G_GGCC'C
Cuts at: 384 498 1625 384
Size: 114 1127 5067
Fragments arranged by size:
5067 1127 114
ApaLI G'TGCA_C
Cuts at: 742 766 5078 742
Size: 24 4312 1972
Fragments arranged by size:
4312 1972 24
ApoI r'AATT_y
Cuts at: 574 3312 5661 5917 574
Size: 2738 2349 256 965
Fragments arranged by size:
2738 2349 965 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6308
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6308
AvaI C'yCGr_G
Cuts at: 197 625 866 979 1002 1100 1216 1344 1357
Size: 428 241 113 23 98 116 128 13
Cuts at: 1357 2475 3328 197
Size: 1118 853 3177
Fragments arranged by size:
3177 1118 853 428 241 128 116 113
98 23 13
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2943 2976 2943
Size: 33 6275
BamHI G'GATC_C
Cuts at: 3333 3333
Size: 6308
BanI G'GyrC_C
Cuts at: 1040 1938 2138 2467 2951 3324 3738 3986 4021
Size: 898 200 329 484 373 414 248 35
Cuts at: 4021 1040
Size: 3327
Fragments arranged by size:
3327 898 484 414 373 329 248 200
35
BanII G_rGCy'C
Cuts at: 384 498 952 1160 1364 1415 1432 1625 2536
Size: 114 454 208 204 51 17 193 911
Cuts at: 2536 2602 2683 3322 4352 384
Size: 66 81 639 1030 2340
Fragments arranged by size:
2340 1030 911 639 454 208 204 193
114 81 66 51 17
BbeI G_GCGC'C
Cuts at: 3742 3990 3742
Size: 248 6060
BbsI GAAGACnn'nnnn_
Cuts at: 625 1075 2267 2441 625
Size: 450 1192 174 4492
Fragments arranged by size:
4492 1192 450 174
BbvCI CC'TCA_GC
Cuts at: 899 1684 899
Size: 785 5523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1986 2020 1986
Size: 34 6274
BciVI GTATCCnnnnn_n'
Cuts at: 4206 4967 6308 4206
Size: 761 1341 4206
Fragments arranged by size:
4206 1341 761
BclI T'GATC_A
Cuts at: 183 3828 183
Size: 3645 2663
BglII A'GATC_T
Cuts at: 3823 5860 3823
Size: 2037 4271
BlpI GC'TnA_GC
Cuts at: 1505 2980 3233 3420 1505
Size: 1475 253 187 4393
Fragments arranged by size:
4393 1475 253 187
Bme1580I G_kGCm'C
Cuts at: 384 498 746 770 862 1625 2315 3933 4026
Size: 114 248 24 92 763 690 1618 93
Cuts at: 4026 5082 384
Size: 1056 1610
Fragments arranged by size:
1618 1610 1056 763 690 248 114 93
92 24
BmrI ACTGGGnnnn_n'
Cuts at: 1061 2036 2255 2940 2951 3520 3601 3936 6080
Size: 975 219 685 11 569 81 335 2144
Cuts at: 6080 1061
Size: 1289
Fragments arranged by size:
2144 1289 975 685 569 335 219 81
11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1937 2533 4675 1937
Size: 596 2142 3570
Fragments arranged by size:
3570 2142 596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 1168 1399 1624 1935 2626 2667 2834 546
Size: 622 231 225 311 691 41 167 4020
Fragments arranged by size:
4020 691 622 311 231 225 167 41
Bpu10I CC'TnA_GC
Cuts at: 899 997 1684 2567 899
Size: 98 687 883 4640
Fragments arranged by size:
4640 883 687 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 1084 3484 4363 4855 5153 432
Size: 652 2400 879 492 298 1587
Fragments arranged by size:
2400 1587 879 652 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 564 6012 564
Size: 5448 860
BsaAI yAC'GTr
Cuts at: 816 3661 816
Size: 2845 3463
BsaBI GATnn'nnATC
Cuts at: 6 2809 3822 5859 6
Size: 2803 1013 2037 455
Fragments arranged by size:
2803 2037 1013 455
BsaHI Gr'CG_yC
Cuts at: 809 938 1795 2708 3739 3987 4689 6185 809
Size: 129 857 913 1031 248 702 1496 932
Fragments arranged by size:
1496 1031 932 913 857 702 248 129
BsaWI w'CCGG_w
Cuts at: 952 2818 3497 4018 4696 4970 5117 5528 952
Size: 1866 679 521 678 274 147 411 1732
Fragments arranged by size:
1866 1732 679 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 637 667 1771 1801 1840 1870 637
Size: 30 1104 30 39 30 5075
Fragments arranged by size:
5075 1104 39 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 643 1022 1423 1534 1672 1795 1893 2964 643
Size: 379 401 111 138 123 98 1071 3987
Fragments arranged by size:
3987 1071 401 379 138 123 111 98
BseYI C'CCAG_C
Cuts at: 825 1588 1617 1885 2909 3076 3152 3733 4491
Size: 763 29 268 1024 167 76 581 758
Cuts at: 4491 5068 5564 825
Size: 577 496 1569
Fragments arranged by size:
1569 1024 763 758 581 577 496 268
167 76 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 667 744 1877 523
Size: 144 77 1133 4954
Fragments arranged by size:
4954 1133 144 77
BsiEI CG_ry'CG
Cuts at: 104 786 1629 2305 2309 3850 3896 5104 104
Size: 682 843 676 4 1541 46 1208 1308
Fragments arranged by size:
1541 1308 1208 843 682 676 46 4
BsiHKAI G_wGCw'C
Cuts at: 746 770 952 1160 1415 2683 3322 4100 4290
Size: 24 182 208 255 1268 639 778 190
Cuts at: 4290 5082 746
Size: 792 1972
Fragments arranged by size:
1972 1268 792 778 639 255 208 190
182 24
BsmI GAATG_Cn'
Cuts at: 294 294
Size: 6308
BsmBI CGTCTCn'nnnn_
Cuts at: 1454 2715 1454
Size: 1261 5047
Bsp1286I G_dGCh'C
Cuts at: 384 498 746 770 862 952 1160 1364 1415
Size: 114 248 24 92 90 208 204 51
Cuts at: 1415 1432 1625 2315 2536 2602 2683 3322 3933
Size: 17 193 690 221 66 81 639 611
Cuts at: 3933 4026 4100 4290 4352 5082 384
Size: 93 74 190 62 730 1610
Fragments arranged by size:
1610 730 690 639 611 248 221 208
204 193 190 114 93 92 90 81
74 66 62 51 24 17
BspEI T'CCGG_A
Cuts at: 952 5528 952
Size: 4576 1732
BspHI T'CATG_A
Cuts at: 1574 5484 1574
Size: 3910 2398
BspMI ACCTGCnnnn'nnnn_
Cuts at: 117 362 1410 1541 2150 3358 3572 3874 4255
Size: 245 1048 131 609 1208 214 302 381
Cuts at: 4255 117
Size: 2170
Fragments arranged by size:
2170 1208 1048 609 381 302 245 214
131
BsrBI CCG'CTC
Cuts at: 50 633 893 4600 4654 6138 6303 50
Size: 583 260 3707 54 1484 165 55
Fragments arranged by size:
3707 1484 583 260 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1971 4220 1971
Size: 2249 4059
BsrFI r'CCGG_y
Cuts at: 785 1496 2135 2380 4306 4487 4696 785
Size: 711 639 245 1926 181 209 2397
Fragments arranged by size:
2397 1926 711 639 245 209 181
BsrGI T'GTAC_A
Cuts at: 706 706
Size: 6308
BssSI C'ACGA_G
Cuts at: 2337 4579 4937 2337
Size: 2242 358 3708
Fragments arranged by size:
3708 2242 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3685 3685
Size: 6308
BstBI TT'CG_AA
Cuts at: 4669 4669
Size: 6308
BstEII G'GTnAC_C
Cuts at: 1812 1812
Size: 6308
BstXI CCAn_nnnn'nTGG
Cuts at: 1051 1500 3084 3123 1051
Size: 449 1584 39 4236
Fragments arranged by size:
4236 1584 449 39
BstYI r'GATC_y
Cuts at: 1 833 3333 3800 3823 4158 4404 5416 5502
Size: 832 2500 467 23 335 246 1012 86
Cuts at: 5502 5514 5860 1
Size: 12 346 449
Fragments arranged by size:
2500 1012 832 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 529 1905 2747 529
Size: 1376 842 4090
Fragments arranged by size:
4090 1376 842
BtgI C'CryG_G
Cuts at: 107 475 886 1433 2525 107
Size: 368 411 547 1092 3890
Fragments arranged by size:
3890 1092 547 411 368
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3213 4258 4439 4602 3213
Size: 1045 181 163 4919
Fragments arranged by size:
4919 1045 181 163
BtsI GCAGTG_nn'
Cuts at: 679 1228 2170 2874 3551 679
Size: 549 942 704 677 3436
Fragments arranged by size:
3436 942 704 677 549
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2191 2226 2191
Size: 35 6273
DraI TTT'AAA
Cuts at: 3307 3307
Size: 6308
DrdI GACnn_nn'nnGTC
Cuts at: 1210 2545 4014 4872 1210
Size: 1335 1469 858 2646
Fragments arranged by size:
2646 1469 1335 858
EaeI y'GGCC_r
Cuts at: 783 1131 1559 1626 2302 2348 3240 3893 4067
Size: 348 428 67 676 46 892 653 174
Cuts at: 4067 4458 4485 5830 783
Size: 391 27 1345 1261
Fragments arranged by size:
1345 1261 892 676 653 428 391 348
174 67 46 27
EagI C'GGCC_G
Cuts at: 783 1626 2302 3893 783
Size: 843 676 1591 3198
Fragments arranged by size:
3198 1591 843 676
EarI CTCTTCn'nnn_
Cuts at: 416 2038 2241 3010 4331 4541 416
Size: 1622 203 769 1321 210 2183
Fragments arranged by size:
2183 1622 1321 769 210 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 121 363 1433 2056 4516 4826 4972 6118 121
Size: 242 1070 623 2460 310 146 1146 311
Fragments arranged by size:
2460 1146 1070 623 311 310 242 146
EcoICRI GAG'CTC
Cuts at: 950 1158 1413 2681 3320 950
Size: 208 255 1268 639 3938
Fragments arranged by size:
3938 1268 639 255 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 406 546 1168 1399 1624 1717 1935 2435
Size: 180 140 622 231 225 93 218 500
Cuts at: 2435 2626 2667 2834 4133 4565 5312 5580 226
Size: 191 41 167 1299 432 747 268 954
Fragments arranged by size:
1299 954 747 622 500 432 268 231
225 218 191 180 167 140 93 41
EcoNI CCTnn'n_nnAGG
Cuts at: 1079 1764 3298 1079
Size: 685 1534 4089
Fragments arranged by size:
4089 1534 685
EcoO109I rG'GnC_Cy
Cuts at: 381 1112 1203 1438 1472 1485 1621 1635 2319
Size: 731 91 235 34 13 136 14 684
Cuts at: 2319 2634 3119 3447 381
Size: 315 485 328 3242
Fragments arranged by size:
3242 731 684 485 328 315 235 136
91 34 14 13
EcoRI G'AATT_C
Cuts at: 574 3312 574
Size: 2738 3570
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4327 4327
Size: 6308
FspI TGC'GCA
Cuts at: 256 4089 256
Size: 3833 2475
HaeII r_GCGC'y
Cuts at: 370 598 977 1680 3742 3990 5012 5991 370
Size: 228 379 703 2062 248 1022 979 687
Fragments arranged by size:
2062 1022 979 703 687 379 248 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 637 669 1144 1176 1771 1803 2413 2445 2743
Size: 32 475 32 595 32 610 32 298
Cuts at: 2743 2775 2913 2945 3079 3111 4150 4180 4182
Size: 32 138 32 134 32 1039 30 2
Cuts at: 4182 4212 637
Size: 30 2733
Fragments arranged by size:
2733 1039 610 595 475 298 138 134
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1719 3347 1719
Size: 1628 4680
HindIII A'AGCT_T
Cuts at: 2997 2997
Size: 6308
KasI G'GCGC_C
Cuts at: 3738 3986 3738
Size: 248 6060
KpnI G_GTAC'C
Cuts at: 2955 3328 2955
Size: 373 5935
MluI A'CGCG_T
Cuts at: 5741 5741
Size: 6308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2078 2561 3065 4979 5163 2078
Size: 483 504 1914 184 3223
Fragments arranged by size:
3223 1914 504 483 184
MscI TGG'CCA
Cuts at: 1133 2350 4069 1133
Size: 1217 1719 3372
Fragments arranged by size:
3372 1719 1217
MslI CAynn'nnrTG
Cuts at: 2089 2504 4297 4424 2089
Size: 415 1793 127 3973
Fragments arranged by size:
3973 1793 415 127
MspA1I CmG'CkG
Cuts at: 477 639 720 888 1229 1435 1602 1688 1838
Size: 162 81 168 341 206 167 86 150
Cuts at: 1838 2664 3156 3283 3419 3733 4093 5106 5351
Size: 826 492 127 136 314 360 1013 245
Cuts at: 5351 477
Size: 1434
Fragments arranged by size:
1434 1013 826 492 360 341 314 245
206 168 167 162 150 136 127 86
81
NaeI GCC'GGC
Cuts at: 1498 2382 4489 1498
Size: 884 2107 3317
Fragments arranged by size:
3317 2107 884
NarI GG'CG_CC
Cuts at: 3739 3987 3739
Size: 248 6060
NcoI C'CATG_G
Cuts at: 107 107
Size: 6308
NgoMIV G'CCGG_C
Cuts at: 1496 2380 4487 1496
Size: 884 2107 3317
Fragments arranged by size:
3317 2107 884
NspI r_CATG'y
Cuts at: 3361 3861 4768 3361
Size: 500 907 4901
Fragments arranged by size:
4901 907 500
PasI CC'CwG_GG
Cuts at: 1801 1801
Size: 6308
PciI A'CATG_T
Cuts at: 4764 4764
Size: 6308
PflMI CCAn_nnn'nTGG
Cuts at: 837 837
Size: 6308
PfoI T'CCnGG_A
Cuts at: 1415 2392 1415
Size: 977 5331
PmeI GTTT'AAAC
Cuts at: 3307 3307
Size: 6308
PmlI CAC'GTG
Cuts at: 816 816
Size: 6308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1840 1872 5447 5479 1840
Size: 32 3575 32 2669
Fragments arranged by size:
3575 2669 32 32
PpuMI rG'GwC_Cy
Cuts at: 1203 1485 1635 2319 3119 1203
Size: 282 150 684 800 4392
Fragments arranged by size:
4392 800 684 282 150
PspOMI G'GGCC_C
Cuts at: 380 494 1621 380
Size: 114 1127 5067
Fragments arranged by size:
5067 1127 114
PspXI vC'TCGA_Gb
Cuts at: 625 1002 625
Size: 377 5931
PstI C_TGCA'G
Cuts at: 644 1080 1538 1601 3355 4040 644
Size: 436 458 63 1754 685 2912
Fragments arranged by size:
2912 1754 685 458 436 63
PvuI CG_AT'CG
Cuts at: 104 2309 104
Size: 2205 4103
PvuII CAG'CTG
Cuts at: 639 1602 1688 3156 3733 4093 639
Size: 963 86 1468 577 360 2854
Fragments arranged by size:
2854 1468 963 577 360 86
RsrII CG'GwC_CG
Cuts at: 4503 4503
Size: 6308
SacI G_AGCT'C
Cuts at: 952 1160 1415 2683 3322 952
Size: 208 255 1268 639 3938
Fragments arranged by size:
3938 1268 639 255 208
SacII CC_GC'GG
Cuts at: 478 889 1436 478
Size: 411 547 5350
Fragments arranged by size:
5350 547 411
SalI G'TCGA_C
Cuts at: 3345 3345
Size: 6308
SapI GCTCTTCn'nnn_
Cuts at: 2038 3010 4331 4541 2038
Size: 972 1321 210 3805
Fragments arranged by size:
3805 1321 972 210
SbfI CC_TGCA'GG
Cuts at: 3355 3355
Size: 6308
ScaI AGT'ACT
Cuts at: 5825 5825
Size: 6308
SfcI C'TryA_G
Cuts at: 33 461 469 640 1076 1534 1597 1754 3351
Size: 428 8 171 436 458 63 157 1597
Cuts at: 3351 4036 5029 5220 33
Size: 685 993 191 1121
Fragments arranged by size:
1597 1121 993 685 458 436 428 191
171 157 63 8
SfoI GGC'GCC
Cuts at: 3740 3988 3740
Size: 248 6060
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6308
SmaI CCC'GGG
Cuts at: 868 981 1102 1359 3330 868
Size: 113 121 257 1971 3846
Fragments arranged by size:
3846 1971 257 121 113
SmlI C'TyrA_G
Cuts at: 197 411 625 1002 1063 3463 4378 4870 5132
Size: 214 214 377 61 2400 915 492 262
Cuts at: 5132 197
Size: 1373
Fragments arranged by size:
2400 1373 915 492 377 262 214 214
61
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6308
SphI G_CATG'C
Cuts at: 3361 3861 3361
Size: 500 5808
StuI AGG'CCT
Cuts at: 203 754 1029 1855 1982 2565 203
Size: 551 275 826 127 583 3946
Fragments arranged by size:
3946 826 583 551 275 127
StyI C'CwwG_G
Cuts at: 107 749 803 2315 2411 2630 3442 3796 107
Size: 642 54 1512 96 219 812 354 2619
Fragments arranged by size:
2619 1512 812 642 354 219 96 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2025 2950 3891 4024 4483 4692 2025
Size: 925 941 133 459 209 3641
Fragments arranged by size:
3641 941 925 459 209 133
TatI w'GTAC_w
Cuts at: 170 706 5823 170
Size: 536 5117 655
Fragments arranged by size:
5117 655 536
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2144 2176 3039 3071 5825 5857 2144
Size: 32 863 32 2754 32 2595
Fragments arranged by size:
2754 2595 863 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4105 4105
Size: 6308
XbaI T'CTAG_A
Cuts at: 1167 3339 1167
Size: 2172 4136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1731 2343 3803 1731
Size: 612 1460 4236
Fragments arranged by size:
4236 1460 612
XhoI C'TCGA_G
Cuts at: 197 625 1002 197
Size: 428 377 5503
Fragments arranged by size:
5503 428 377
XmaI C'CCGG_G
Cuts at: 866 979 1100 1357 3328 866
Size: 113 121 257 1971 3846
Fragments arranged by size:
3846 1971 257 121 113
XmnI GAAnn'nnTTC
Cuts at: 311 2032 311
Size: 1721 4587
ZraI GAC'GTC
Cuts at: 2709 2709
Size: 6308
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AscI AvrII BfrBI BglI
BmgBI BmtI BsiWI BssHII BstZ17I ClaI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI